CN110038153A - A kind of medical hemostatic bibre material and preparation method thereof - Google Patents
A kind of medical hemostatic bibre material and preparation method thereof Download PDFInfo
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- CN110038153A CN110038153A CN201910236964.9A CN201910236964A CN110038153A CN 110038153 A CN110038153 A CN 110038153A CN 201910236964 A CN201910236964 A CN 201910236964A CN 110038153 A CN110038153 A CN 110038153A
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- bibre
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
- A61L15/325—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0036—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/043—Mixtures of macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/102—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention relates to the technical fields of medical material, and in particular to a kind of medical hemostatic bibre material and preparation method thereof.Mechanical equipment should directly be passed through by medical sthptic sponge material to be crushed, the medical bio fibrous material with certain fibre diameter is made.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain the activity of original biomaterial, with bigger surface area, any shape can be compressed into, it can promote wound reparation, has the effect of general hemostatic sponge material, with more the advantage flexibly used, wound can be directly sprayed on and reach quickly absorption Wound exudate, it can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, solves the problems such as operative hemorrhages such as the surface of a wound, the bleeding of large area diffusivity, internal cavity, the pipeline of irregular shape, debridement, filling.The characteristics of medical hemostatic bibre material and preparation method thereof has simple and easy to do, at low cost advantage, is suitable for the industrialized production of medical hemostatic bibre material.
Description
Technical field
The present invention relates to the technical fields of medical material, and in particular to a kind of medical hemostatic bibre material and its preparation side
Method.
Background technique
In wound first aid and common surgical operation, surface of a wound bleeding is FAQs.Threat to life is excessively understood in blood loss, simultaneously
Increase infection chance, reduces success rate of operation.Therefore, hemostasis means appropriate can significant shortening operating time, for wound
Or post-operative recovery has importance.There are many hemostasis means, according to the position of bleeding, perform the operation chamber size and body compatibility,
Coagulation function and economic condition of patient etc., clinically conventional machinery Hemostasis as suture, ligation, coagulation etc. or use its
His hemostatic material such as tourniquet, bandage, medical sponge, cotton swab, gauze etc..However, traditional hemostatic material is in face of complexity
Wound location and large area diffusivity bleeding when do not have preferable haemostatic effect.Ideal hemostatic material should have quickly
Hemostasis, without infection risk, it is safe and non-stimulating, do not influence organization healing, not readily dissolve, degradation is fast, controllably.In recent years, with doctor
With the further investigation of biological hemostatic material, a large amount of high molecular material, such as: collagen, fibroin albumen, soybean protein, wheat
Albumen, hyaluronic acid, polycaprolactone, gelatin, chitosan, cellulose, polylactic acid, polyethylene glycol, nano micro crystal cellulose, water-setting
Glue etc. has immunogenicity low, and tensile strength is good, hemostatic function is strong, biodegradable is good, safe and non-toxic nonirritant, and
It is widely used in the various styptic sponge products of manufacture.However, for wound irregular in operation or large area diffusivity bleeding, only
Sea of blood silk floss is unable to deep contact with the wound that coincide, and clinical application has significant limitation.
In view of the limitation of traditional hemostatic material, it is necessary to develop a kind of novel efficient hemostatic material, such as have larger
The stanch fibre material of surface area, it has also become new research hotspot.Someone collects the short fibre of nanometer using electron spray, electrical spinning method
Dimension cuts off spinning by the way that a stirring revolving fragment is added near spray head, to obtain required nanofiber.This method can be received
Collect nanometer to micron level fiber, but due to consideration that can spinnability, need the spinning solution of higher concentration, be easy blocking spray
Head, while joined organic solvent in spinning solution, it can lead to the Partial digestion of material, if later period cleaning and solvent volatilization are bad,
Toxic solvent residual is easily caused, high-pressure installation is also possible to generate the risk of burning.Someone uses 0.3%~2% collagen
Solution is as water phase, and atoleine is as oily phase, handles point by with water W/O emulsifier system, then through water-miscible organic solvent
From obtaining collagen stanch fibre, but this method cleaning separation is more difficult, is also easy to cause organic solvent residual.Side of the present invention
Method passes through mechanical crushing, grinding directly by traditional medical sthptic sponge material to get novel stanch fibre.Pass through machinery
It crushes, the novel stanch fibre that polishing is prepared, the activity of original biomaterial can be maintain, there is bigger surface area,
It can be compressed into any shape, wound reparation is can promote, have the effect of general hemostatic sponge material, with more flexibly making
Advantage, can directly be sprayed on wound reach quickly absorb Wound exudate except can be performed the operation with intracoelomic cavity, pipeline etc.
Hemostasis etc. solves operative hemorrhages and the debridements such as the surface of a wound of irregular shape, the appearance of large area diffusivity, internal cavity, pipeline,
The problems such as filling.With the preparation method of conventional medical hemostatic bibre material, as Electrospun, electron spray, W/O emulsification system pass through again
It crosses water-miscible organic solvent processing separation prepared powdery or villiform azelon etc. to compare, avoids and prepare
Stanch fibre material is because solvent is to the drawbacks such as the degradation of hemostatic material and reagent residual.This method is simple and easy to do, at low cost, is suitable for
In the industrialized production of new medical stanch fibre material.
Summary of the invention
It is an object of the present invention in view of the deficiencies of the prior art, provide a kind of preparation of medical hemostatic bibre material
Method, the preparation method of the medical hemostatic bibre material eliminate various high pressures or chemical reagent using mechanical crushing method
Dissolution is precipitated, repeatedly the operating procedures such as cleaning, and production procedure is simple, is easy to industrialization.
The second object of the present invention is in view of the deficiencies of the prior art, to provide a kind of medical hemostatic bibre material, the doctor
Have the advantages that preparation method is simple with stanch fibre material, and medical hemostatic bibre material obtained maintains original biological material
The activity of material has bigger surface area, can be compressed into any shape, can promote wound reparation, with more what is flexibly used
Advantage.
One of to achieve the goals above, the present invention adopts the following technical scheme:
A kind of preparation method of medical hemostatic bibre material is provided, is directly to pass through machinery by medical sthptic sponge material to set
It is standby to be crushed, the medical bio fibrous material with certain fibre diameter is made.
In above-mentioned technical proposal, the hemostatic sponge material be collagen, fibroin albumen, soybean protein, wheat gluten,
In hyaluronic acid, polycaprolactone, gelatin, chitosan, cellulose, polylactic acid, polyethylene glycol, nano micro crystal cellulose or hydrogel
One or more kinds of compositions.
In above-mentioned technical proposal, the mechanical equipment is ultrafine crusher, homogenate cooking machine, liquid nitrogen frozen pulverizer, low
The mechanical combination of one or more of warm freezing crusher, superfreeze pulverizer or cold wind freezing crusher crushes
Machine.
In above-mentioned technical proposal, the ultrafine crusher and homogenate cooking machine are optional due to generating higher operating temperature
It selects and crushes repeatedly, the interval time crushed every time is 3s~60s pause, and so as to material heat dissipation, the temperature to material drops to room
Wen Hou continues to crush, and until material reaches required size, obtains the medical hemostatic bibre material.
In above-mentioned technical proposal, the cryogenic freezing pulverizer needs to judge whether according to the actual situation that pause is general and dissipates
Heat is suspended if generating higher operating temperature to radiate, if not generating higher operating temperature, does not need temporarily
Stop radiating.
In above-mentioned technical proposal, fibre diameter size, that is, made required for the liquid nitrogen frozen pulverizer is directly ground to
Obtain the medical hemostatic bibre material.
In above-mentioned technical proposal, described crush grinds comminuting method, air-flowing type for dry grinding comminuting method, chopping microtomy, supper fine refined
Ultramicro grinding method or the combination comminuting method for one or more of blending method.
In above-mentioned technical proposal, the power of the crushing is 50W~2000W, and the frequency of the crushing is 40HZ~65HZ,
The revolving speed when crushing is 10000 revs/min~50000 revs/min.
In above-mentioned technical proposal, the fibre diameter is 400nm~1mm.
To achieve the goals above two, the present invention adopts the following technical scheme:
A kind of medical hemostatic bibre material is provided, is the preparation side according to a kind of medical hemostatic bibre material described above
Medical hemostatic bibre material obtained by method.
Compared with prior art, beneficial effect is the present invention:
(1) preparation method of a kind of medical hemostatic bibre material provided by the invention, it is direct by medical sthptic sponge material
It is crushed by mechanical equipment, obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized,
The activity of original biomaterial is maintain, there is bigger surface area, any shape can be compressed into, can promote wound reparation,
Have the effect of general hemostatic sponge material, with more the advantage flexibly used, can directly be sprayed on wound and reach fast
Speed absorbs Wound exudate, can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, solves the surface of a wound, big of irregular shape
The problems such as operative hemorrhages such as the bleeding of area diffusivity, internal cavity, pipeline, debridement, filling.With conventional medical hemostatic bibre material
The preparation method of material, as Electrospun, electron spray, W/O emulsification system are handled prepared by separation using water-miscible organic solvent
Powdery or villiform azelon etc. compare, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to only
The drawbacks such as the degradation of blood material and reagent residual.
(2) preparation method of a kind of medical hemostatic bibre material provided by the invention, have it is simple and easy to do, at low cost is excellent
Point eliminates various high pressures or chemical reagent dissolution, is precipitated, repeatedly the operating procedures such as cleaning, and production procedure is simple, is suitable for
In the industrialized production of medical hemostatic bibre material.
(3) a kind of medical hemostatic bibre material provided by the invention, maintains the activity of original biomaterial, has bigger
Surface area, any shape can be compressed into, can promote wound reparation, with more the advantage flexibly used.
Detailed description of the invention
Fig. 1 is a kind of outside drawing of medical hemostatic bibre material made from the embodiment of the present invention 1.
Fig. 2 is a kind of scanning electron microscope diagram of medical hemostatic bibre material made from the embodiment of the present invention 1.
Fig. 3 is a kind of infrared spectrogram of medical hemostatic bibre material made from the embodiment of the present invention 1.
Fig. 4 is a kind of thermal stability (DSC) analysis chart of medical hemostatic bibre material made from the embodiment of the present invention 1.
Fig. 5 is that a kind of medical hemostatic bibre material made from the embodiment of the present invention 1 is applied to rabbit auricular vein hemostasis knot
Fruit figure.
Fig. 6 is that a kind of medical hemostatic bibre material made from the embodiment of the present invention 1 is external applied to rabbit new blood
Blood coagulation situation map.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain
The present invention is not intended to limit the present invention.
Embodiment 1.
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 1um~72um is made.
In the present embodiment, hemostatic sponge material is collagen.
In the present embodiment, mechanical equipment is homogenate cooking machine.
Wherein, homogenate cooking machine may be selected to crush repeatedly due to generating higher operating temperature, when the interval crushed every time
Between for 3s~60s suspend, so as to material heat dissipation, after the temperature of material drops to room temperature, continue to crush, until material reaches institute
The size needed obtains medical hemostatic bibre material.
In the present embodiment, crushes and grind comminuting method for dry grinding comminuting method and supper fine refined and be used in combination；Every crushing 3s pause is about
1min is crushed repeatedly, until reaching required fibre diameter.
In the present embodiment, the power of crushing is 250W, and the frequency of the crushing is 50HZ, and the revolving speed when crushing is
20000 revs/min.
A kind of preparation method of medical hemostatic bibre material of the present embodiment, directly passes through machine by medical sthptic sponge material
Tool equipment crushes, and obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain
The activity of original biomaterial has bigger surface area, can be compressed into any shape, can promote wound reparation, have
The effect of general hemostatic sponge material, can directly be sprayed on wound and reach quick absorption with more the advantage flexibly used
Wound exudate can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, and the surface of a wound that solves irregular shape, large area are more
The problems such as operative hemorrhages such as unrestrained property bleeding, internal cavity, pipeline, debridement, filling.With the system of conventional medical hemostatic bibre material
Preparation Method, as Electrospun, electron spray, W/O emulsification system handle the prepared powdery of separation using water-miscible organic solvent
Or villiform azelon etc. is compared, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to hemostatic material
Degradation and reagent residual etc. drawbacks.
Embodiment 2.
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 500nm~100um is made.
In the present embodiment, hemostatic sponge material is the composition of collagen and fibroin albumen.
In the present embodiment, mechanical equipment is liquid nitrogen frozen pulverizer.
Wherein, fibre diameter size, obtains medical hemostatic bibre required for liquid nitrogen frozen pulverizer is directly ground to
Material.
In the present embodiment, the power of crushing is 1000W, and the frequency of the crushing is 40HZ, and the revolving speed when crushing is
20000 revs/min.
A kind of preparation method of medical hemostatic bibre material of the present embodiment, directly passes through machine by medical sthptic sponge material
Tool equipment crushes, and obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain
The activity of original biomaterial has bigger surface area, can be compressed into any shape, can promote wound reparation, have
The effect of general hemostatic sponge material, can directly be sprayed on wound and reach quick absorption with more the advantage flexibly used
Wound exudate can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, and the surface of a wound that solves irregular shape, large area are more
The problems such as operative hemorrhages such as unrestrained property bleeding, internal cavity, pipeline, debridement, filling.With the system of conventional medical hemostatic bibre material
Preparation Method, as Electrospun, electron spray, W/O emulsification system handle the prepared powdery of separation using water-miscible organic solvent
Or villiform azelon etc. is compared, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to hemostatic material
Degradation and reagent residual etc. drawbacks.
Embodiment 3.
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 400nm~1mm is made.
In the present embodiment, hemostatic sponge material is the composition of collagen and hyaluronic acid.
In the present embodiment, mechanical equipment is cryogenic freezing pulverizer.
Wherein, cryogenic freezing pulverizer needs to judge whether to suspend general heat dissipation according to the actual situation, if generated higher
Operating temperature, then pause to radiate, if not generating higher operating temperature, do not need pause heat dissipation.
In the present embodiment, crushes and grind comminuting method for supper fine refined.
In the present embodiment, the power of crushing is 150W, and the frequency of the crushing is 50HZ, and revolving speed when crushing is 23000
Rev/min.
A kind of preparation method of medical hemostatic bibre material of the present embodiment, directly passes through machine by medical sthptic sponge material
Tool equipment crushes, and obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain
The activity of original biomaterial has bigger surface area, can be compressed into any shape, can promote wound reparation, have
The effect of general hemostatic sponge material, can directly be sprayed on wound and reach quick absorption with more the advantage flexibly used
Wound exudate can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, and the surface of a wound that solves irregular shape, large area are more
The problems such as operative hemorrhages such as unrestrained property bleeding, internal cavity, pipeline, debridement, filling.With the system of conventional medical hemostatic bibre material
Preparation Method, as Electrospun, electron spray, W/O emulsification system handle the prepared powdery of separation using water-miscible organic solvent
Or villiform azelon etc. is compared, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to hemostatic material
Degradation and reagent residual etc. drawbacks.
Embodiment 4.
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 400nm~1mm is made.
In the present embodiment, hemostatic sponge material is the composition of soybean protein and wheat gluten.
In the present embodiment, mechanical equipment is superfreeze pulverizer.
In the present embodiment, crush as chopping microtomy.
In the present embodiment, the power of crushing is 2000W, and the frequency of the crushing is 65HZ, and revolving speed when crushing is 50000
Rev/min.
A kind of preparation method of medical hemostatic bibre material of the present embodiment, directly passes through machine by medical sthptic sponge material
Tool equipment crushes, and obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain
The activity of original biomaterial has bigger surface area, can be compressed into any shape, can promote wound reparation, have
The effect of general hemostatic sponge material, can directly be sprayed on wound and reach quick absorption with more the advantage flexibly used
Wound exudate can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, and the surface of a wound that solves irregular shape, large area are more
The problems such as operative hemorrhages such as unrestrained property bleeding, internal cavity, pipeline, debridement, filling.With the system of conventional medical hemostatic bibre material
Preparation Method, as Electrospun, electron spray, W/O emulsification system handle the prepared powdery of separation using water-miscible organic solvent
Or villiform azelon etc. is compared, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to hemostatic material
Degradation and reagent residual etc. drawbacks.
Embodiment 5.
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 400nm~1mm is made.
In the present embodiment, hemostatic sponge material is the composition of polylactic acid, nano micro crystal cellulose and hydrogel.
In the present embodiment, mechanical equipment is cold wind freezing crusher.
In the present embodiment, crush as the broken method of air blast ultramicro powder.
In the present embodiment, the power of crushing is 1000W, and the frequency of crushing is 45HZ, revolving speed when crushing is 10000 turns/
Point.
A kind of preparation method of medical hemostatic bibre material of the present embodiment, directly passes through machine by medical sthptic sponge material
Tool equipment crushes, and obtains medical hemostatic bibre material.By the way that the medical hemostatic bibre material prepared is mechanically pulverized, maintain
The activity of original biomaterial has bigger surface area, can be compressed into any shape, can promote wound reparation, have
The effect of general hemostatic sponge material, can directly be sprayed on wound and reach quick absorption with more the advantage flexibly used
Wound exudate can be also used for the hemostasis of the operations such as intracoelomic cavity, pipeline, and the surface of a wound that solves irregular shape, large area are more
The problems such as operative hemorrhages such as unrestrained property bleeding, internal cavity, pipeline, debridement, filling.With the system of conventional medical hemostatic bibre material
Preparation Method, as Electrospun, electron spray, W/O emulsification system handle the prepared powdery of separation using water-miscible organic solvent
Or villiform azelon etc. is compared, the invention can avoid the medical hemostatic bibre materials prepared because solvent is to hemostatic material
Degradation and reagent residual etc. drawbacks.
Embodiment 6
A kind of preparation method of medical hemostatic bibre material, be directly passed through by medical sthptic sponge material mechanical equipment into
Row crushes, and the medical bio fibrous material that fibre diameter is 400nm~1mm is made.
In the present embodiment, hemostatic sponge material is collagen, fibroin albumen, soybean protein, wheat gluten, hyalomitome
One of acid, polycaprolactone, gelatin, chitosan, cellulose, polylactic acid, polyethylene glycol, nano micro crystal cellulose or hydrogel
Or two or more compositions.
Wherein, mechanical equipment is ultrafine crusher, homogenate cooking machine, liquid nitrogen frozen pulverizer, cryogenic freezing pulverizer, surpasses
The mechanical combination pulverizer of one or more of cryogenic freezing pulverizer or cold wind freezing crusher.
Wherein, ultrafine crusher and homogenate cooking machine may be selected to crush repeatedly, every time due to generating higher operating temperature
The interval time of crushing is that 3s~60s pause after the temperature of material drops to room temperature, continues to crush, directly so as to material heat dissipation
Reach required size to material, obtains the medical hemostatic bibre material.
Wherein, cryogenic freezing pulverizer needs to judge whether to suspend general heat dissipation according to the actual situation, if generated higher
Operating temperature, then pause to radiate, if not generating higher operating temperature, do not need pause heat dissipation.
Wherein, fibre diameter size, obtains the medical hemostatic required for liquid nitrogen frozen pulverizer is directly ground to
Fibrous material.
Wherein, it crushes and grinds comminuting method, the broken method of air blast ultramicro powder for dry grinding comminuting method, chopping microtomy, supper fine refined or stir
The combination comminuting method of one or more of broken method.
Wherein, the power of crushing is 50W~2000W, and the frequency of the crushing is 40HZ~65HZ, and revolving speed when crushing is
10000 revs/min~50000 revs/min.
Measuring:
The content of hydroxyproline is measured to a kind of medical hemostatic bibre material made from embodiment 1.Hydroxyproline is I
Collagen type characteristic amino acid is provided according to YY/T 1511-2017, is detected using this method, the amount of hydroxyproline must not
Less than 9%.Using the absorbance at 560nm as ordinate (Y), reference substance content is that abscissa (X) carries out linear regression, must be returned
Equation: Y=0.0482X+0.0007, r=0.9997.Its measurement result refers to table 1.
The hydroxyproline content of 1 azelon of table measures
As shown in Table 1, a kind of medical hemostatic bibre material made from embodiment 1 is containing hydroxyproline
11.54%, it complies with standard, purity is high.
The measurement of blood compatibility is carried out to a kind of medical hemostatic bibre material made from embodiment 1, measurement result is asked
Referring to table 2.
The fibrinous hemolysis rate of table 2
As shown in Table 2, eventually by the absorbance at measurement 545nm, the hemolysis rate of azelon is write down, it is molten
Blood rate < 5%.
Phenetic analysis:
(1) infrared spectrum analysis
Infrared spectrum analysis is carried out to a kind of medical hemostatic bibre material made from embodiment 1, as shown in figure 3, infrared
In spectrum atlas, distinctive amino and imido based structures are mainly shown as on the distinctive triple-helix structure of collagen and its molecule
4 characteristic absorption peaks, i.e. amide A band at 3299cm-1~3330cm-1, the amide B band at 2877cm-1~3084cm-1,
II band of amide at amide Ⅰ and 1560cm-1~1540cm-1 at 1661cm-1~1636cm-1.In Fig. 3, azelon
It is N-H stretching vibration near 3301cm-1 in infrared spectrum, 1640cm-1 is nearby the C=O stretching vibration of amide Ⅰ,
1544cm-1 is nearby the N-H bending vibration of II band of amide, and the absorption p-ratio near 1450cm-1~1230cm-1 is shown
The integrality of collagen triple helix structure, the ratio of 1230cm-1 and 1450cm-1 peak intensity are 0.89, are collagen
Characteristic value.It follows that the substance of this experiment measurement is I-type collagen, and the triple helix structure of collagen has saved
It is whole.
(2) thermal stability analysis
Thermal stability analysis is carried out to a kind of medical hemostatic bibre material made from embodiment 1, as shown in figure 4, thermostabilization
Property (DSC) analysis, as the temperature rises, the weightless process of different regenerated collagens is roughly the same, can be divided into two
Stage: first is that 80 DEG C~90 DEG C, which is caused by being adsorbed on the volatilization of collagen surface and some free hydrones；
Second is that the weightless reason in this stage is mainly the peptide in collagen peptide chain under the action of high temperature between 315 DEG C~330 DEG C
Key is broken, and is degraded to polypeptide and amino acid, final deamination, dehydration.From weight-loss curve (Fig. 4, c curve) as can be seen that warp
The final quality retention of azelon after degradation has decline.The loss of weight is mainly by losing institute in material
The water of the different conditions contained, finally remaining is the quality of residual carbon, and the reduction of quality retention illustrates that albumen water content increases
It is more.Weight-loss ratio curve (Fig. 4, d curve) has intuitively reacted the most fast decomposition temperature and decomposition rate of azelon, wherein albumen
The fiber first stage quickly lose in conjunction with water temperature be 81.55 DEG C；Second stage fast degradation be polypeptide and amino acid, finally
Deamination, dehydration temperature be 315.10 DEG C.
Experimental applications:
Stop blooding to a kind of medical hemostatic bibre material made from embodiment 1 applied to rabbit auricular vein, hemostasis result is shown in
Shown in Fig. 5, to the effect of the auricular vein hemostasis observation hemostasis of rabbit, compared with blank control group (gauze), collagen is fine
It ties up obviously more preferable than blank group haemostatic effect.
And the external blood coagulation situation of rabbit new blood is applied to a kind of medical hemostatic bibre material made from embodiment 1
Observation, as shown in Figure 6, collagen fabric is good to rabbit extracorporeal blood coagulation result for blood coagulation situation, compared to the blank group, statistics
There were significant differences.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of range is protected, although explaining in detail referring to preferred embodiment to the present invention, those skilled in the art are answered
Work as understanding, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the reality of technical solution of the present invention
Matter and range.
Claims (10)
1. a kind of preparation method of medical hemostatic bibre material, it is characterised in that: directly passed through by medical sthptic sponge material
Mechanical equipment is crushed, and the medical bio fibrous material with certain fibre diameter is made.
2. a kind of preparation method of medical hemostatic bibre material according to claim 1, it is characterised in that: the hemostasis sea
Continuous material is collagen, fibroin albumen, soybean protein, wheat gluten, hyaluronic acid, polycaprolactone, gelatin, chitosan, fibre
Tie up one or more of element, polylactic acid, polyethylene glycol, nano micro crystal cellulose or hydrogel composition.
3. a kind of preparation method of medical hemostatic bibre material according to claim 1, it is characterised in that: the machinery is set
Standby is ultrafine crusher, homogenate cooking machine, liquid nitrogen frozen pulverizer, cryogenic freezing pulverizer, superfreeze pulverizer or cold
The mechanical combination pulverizer of one or more of wind freezing crusher.
4. a kind of preparation method of medical hemostatic bibre material according to claim 3, it is characterised in that: the superfine powder
Broken machine and homogenate cooking machine may be selected to crush repeatedly, the interval time crushed every time is 3s due to generating higher operating temperature
~60s pause after the temperature of material drops to room temperature, continues to crush so as to material heat dissipation, required for material reaches
Size obtains the medical hemostatic bibre material.
5. a kind of preparation method of medical hemostatic bibre material according to claim 3, it is characterised in that: the low temperature cold
Freeze pulverizer, need to judge whether to suspend general heat dissipation according to the actual situation, if generating higher operating temperature, pause with
Just it radiates, if not generating higher operating temperature, does not need pause heat dissipation.
6. a kind of preparation method of medical hemostatic bibre material according to claim 3, it is characterised in that: the liquid nitrogen is cold
Freeze fibre diameter size required for pulverizer is directly ground to, obtains the medical hemostatic bibre material.
7. a kind of preparation method of medical hemostatic bibre material according to claim 1, it is characterised in that: the crushing is
Dry grinding comminuting method, chopping microtomy, supper fine refined mill comminuting method, the broken method of air blast ultramicro powder or blend one or both of method with
On combination comminuting method.
8. a kind of preparation method of medical hemostatic bibre material according to claim 1, it is characterised in that: the crushing
Power is 50W~2000W, and the frequency of the crushing is 40HZ~65HZ, the revolving speed when crushing is 10000 revs/min~
50000 revs/min.
9. a kind of preparation method of medical hemostatic bibre material according to claim 1, it is characterised in that: the fiber is straight
Diameter is 400nm~1mm.
10. a kind of medical hemostatic bibre material, it is characterised in that: be according to claim 1 to a kind of doctor described in 9 any one
Medical hemostatic bibre material obtained by preparation method with stanch fibre material.
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| CN107376000A (en) * | 2017-07-14 | 2017-11-24 | 广州迈普再生医学科技有限公司 | Microfibre state hemostatic material and preparation method thereof and hemostatic article |
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| WO2005004928A2 (en) * | 2003-04-04 | 2005-01-20 | W.R. Grace & Co.-Conn. | Porous particulate collagen sponges |
| CN103721247A (en) * | 2014-01-09 | 2014-04-16 | 北京华信佳音医疗科技发展有限责任公司 | Collagen-based composite hemostasis powder and preparation method thereof |
| CN105194712A (en) * | 2014-05-29 | 2015-12-30 | 成都吉泰医疗器械有限公司 | Hemostatic material and preparation method thereof |
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Application publication date: 20190723 |