CN110004096A - One lactobacillus plantarum and its application - Google Patents
One lactobacillus plantarum and its application Download PDFInfo
- Publication number
- CN110004096A CN110004096A CN201910374142.7A CN201910374142A CN110004096A CN 110004096 A CN110004096 A CN 110004096A CN 201910374142 A CN201910374142 A CN 201910374142A CN 110004096 A CN110004096 A CN 110004096A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- microbial bacterial
- bacterial agent
- water body
- aquatic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 58
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 56
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 56
- 230000001580 bacterial effect Effects 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 241000371997 Eriocheir sinensis Species 0.000 claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 25
- 230000000813 microbial effect Effects 0.000 claims abstract description 23
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 11
- 238000009360 aquaculture Methods 0.000 claims description 8
- 244000144974 aquaculture Species 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 6
- 241000607534 Aeromonas Species 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 230000000091 immunopotentiator Effects 0.000 claims description 5
- 241000607528 Aeromonas hydrophila Species 0.000 claims description 4
- 241000191967 Staphylococcus aureus Species 0.000 claims description 4
- 241000607618 Vibrio harveyi Species 0.000 claims description 4
- 230000001717 pathogenic effect Effects 0.000 claims description 4
- 241000607598 Vibrio Species 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 206010047400 Vibrio infections Diseases 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 16
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 15
- 241001465754 Metazoa Species 0.000 abstract description 11
- 238000009313 farming Methods 0.000 abstract description 4
- 230000036737 immune function Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 17
- 238000012360 testing method Methods 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 108020004465 16S ribosomal RNA Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 6
- 235000018291 probiotics Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 241000186660 Lactobacillus Species 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241000371986 Eriocheir Species 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000040710 Chela Species 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000002434 immunopotentiative effect Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 238000013081 phylogenetic analysis Methods 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 241000186046 Actinomyces Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IPRNXEUYCDWBTL-UHFFFAOYSA-N ON=O.ON=O.ON=O.N.N Chemical compound ON=O.ON=O.ON=O.N.N IPRNXEUYCDWBTL-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 244000037640 animal pathogen Species 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 210000000476 body water Anatomy 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000005713 exacerbation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000556 factor analysis Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009654 indole test Methods 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000019086 sulfide ion homeostasis Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K2035/11—Medicinal preparations comprising living procariotic cells
- A61K2035/115—Probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Hydrology & Water Resources (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a lactobacillus plantarum CB5, which is preserved in China typical culture collection center, and the deposit date is on April 8th, 2019, deposit number was CCTCC NO:M 2019244.The invention also discloses a kind of microbial bacterial agents.The invention also discloses the applications of lactobacillus plantarum CB5 and its microbial bacterial agent, lactobacillus plantarum CB5 and its microbial bacterial agent are significant for the bacteriostasis of aquatic pathogenic bacteria, and the immune function for improving Eriocheir sinensis, and the content of ammonia nitrogen and nitrite in Aquatic farming animals water body is significantly reduced, have the function of purifying water body.
Description
Technical field
The invention belongs to aquaculture technology and ecological environmental protection technical field, be related to a kind of drop ammonia nitrogen nitrite and
The function bacterium of antibacterial a, and in particular to lactobacillus plantarum and its application.
Background technique
Eriocheir sinensis (Eriocheir sinensis, be commonly called as river crab) is the distinctive aquatic products treasure in China, is outlet wound
One of the main aquaculture kind converged has the cultivation of large area in the area such as Jiangsu, Zhejiang, Anhui, and 2016 annual outputs are
Up to 81.2MT.However in intensive culture, since Chinese eriocheir sinensises feed protein content is high, aquaculture model is rough, feed
Protein utilization rate is low, and breeding wastewater has the characteristics that nitrogen and phosphorus content is high.Under high-density breeding environment, Eriocheir sinensis waste water
Pollutant include residual bait, nutriment i.e. N, P contained in excrement and excreta etc. since cultivation density increases, feeding increases
More, nitrifier nitrification balance is destroyed, therefore ammonia nitrogen and nitrite concentration constantly rise, and have become Eriocheir sinensis
Major stress factors in production process.Meanwhile during Eriocheir sinensis, there is the nutrients of about 70-85% to pass through
Directly or indirectly mode is discharged into water body, and Eriocheir sinensis excreta and residual bait are deposited in water, rapid at a suitable temperature
It decomposes, increases ammonia nitrogen and nitrite equal size in water body, and then make the culturing pool deterioration of the ecological environment, be easy to cause China
The exacerbation that Eriocheir disease occurs also is easy for causing the increase of aquatic animal medicine usage amount, medicament residue, food peace in this way
The a series of problem of congruence.In Eriocheir sinensis compact district, the unordered discharge of breeding wastewater has become surrounding body richness
The important origin cause of formation of nutrient laden has seriously affected villages and small towns resident living and industrial water quality and safety, and has become and restrict Sinensis
The restrictive factor of chela crab aquaculture sustainable health development.Therefore, it develops and improves Chinese eriocheir sinensises feed utilization rate, drop
The product of low water body ammonia nitrogen and nitrite is an extremely urgent task.
Solve the most common disease and quality problem in intensive culture, common solution have vaccine immunity,
Immunopotentiating agent and probiotics micro-ecological formulation are added in feed.But vaccine develops because its is cumbersome by certain resistance
Hinder, immunopotentiating agent is also limited only to animal body Immune-enhancing effect, changes the help for having no matter to water body.From 1989
After Fuller proposes the probiotics (EM) for allowing people to receive extensively definition, people deepen continuously to the research of probiotics, find prebiotic
The probiotics such as bacterium such as bacillus, lactic acid bacteria, actinomyces have purifying aquaculture water quality, improve and restore breeding water body
Ecological environment；Enteron aisle germ can effectively be inhibited and improve aquatic livestock immunity function, improve the disease resistance of animal, and energy
Aquatic livestock is improved to the digestion and absorption of feed and promotes to grow.Lactic acid bacteria is the common bacteria and symbiosis of people and animal gastrointestinal tract
Bacterium, for a long time, lactic acid bacteria have been widely used in the industries such as food, beverage and probiotics as important probiotics,
It is acknowledged as safe level microbe.It is the novel green additive that developed recently gets up, having improves cultivated animals alimentary canal
Profitable strain inhibits or kills harmful bacteria, improves animal health level, promote animal growth and improve efficiency of feed utilization etc.
Effect.In addition, it also has the characteristics that noresidue, has no toxic side effect, improves breeding ecological environment, reach the mesh of bionomic control
, make breeding production benign development, obtains better economic benefit and ecological benefits.The invention patent is to mention in this context
Out.
Summary of the invention
Goal of the invention: the deficiencies in the prior art are directed to, the present invention provides a lactobacillus plantarum CB5.
Technical problem to be solved by the invention is to provide a kind of microbial bacterial agents.
Also there is provided lactobacillus plantarum CB5, microbial bacterial agents in aquaculture for technical problems to be solved by the present invention
Application.
There is provided lactobacillus plantarum CB5, microbial bacterial agents in preparation killing water for the present invention also technical problems to be solved
Produce the application in animal pathogen reagent or drug.
Also there is provided lactobacillus plantarum CB5, the microbial bacterial agents to prepare for technical problems to be solved by the present invention
Application in immunopotentiator.
Also there is provided lactobacillus plantarum CB5, the microbial bacterial agents to purify for technical problems to be solved by the present invention
Application in water body.
The last technical problems to be solved of the present invention are a kind of Water body cleansing agents, for reducing in Aquatic farming animals water body
The content of ammonia nitrogen and nitrite.
Technical solution: to realize the above-mentioned technical purpose, the invention adopts the following technical scheme: the present invention provides one plant
Lactobacillus plantarum CB5, the lactobacillus plantarum CB5 be it is isolated from Eriocheir sinensis enteron aisle, be now preserved in Chinese Typical Representative
Culture collection, classification naming are lactobacillus plantarum CB5 (Lactobacillus plantarum CB5), preservation day
Phase is on April 8th, 2019, and deposit number is CCTCC NO；M 2019244, preservation address are as follows: Wuhan University's Chinese Typical Representative culture
Object collection.
The content of present invention further includes a kind of microbial bacterial agent, and the microbial bacterial agent includes the lactobacillus plantarum CB5.
The content of present invention further includes the lactobacillus plantarum CB5, the microbial bacterial agent answering in aquaculture
With.
Since lactobacillus plantarum CB5 has the bacteriostasis of aquatic pathogenic bacteria, and the content of present invention further includes described
Lactobacillus plantarum CB5, the microbial bacterial agent inhibits in preparation or kills in aquatic pathogenic bacteria reagent or drug
Using.
Wherein, the aquatic pathogenic bacteria includes Aeromonas hydrophila TPS-30, Aeromonas hydrophila BSK-10, China
One of the pathogenic aeromonas CL99909 of Eriocheir, staphylococcus aureus, vibrio parahaemolytious or Vibrio harveyi are several
Kind.
The content of present invention further includes that the lactobacillus plantarum CB5, the microbial bacterial agent are preparing immunopotentiator
In application.
The content of present invention further includes the fermentation liquid that the immunopotentiator includes lactobacillus plantarum CB5.Using institute of the present invention
The fermentation liquid for stating lactobacillus plantarum CB5 can be used for improving the immune of the immune function of aquatic livestock, especially Eriocheir sinensis
Function.
The content of present invention further includes the lactobacillus plantarum CB5, the microbial bacterial agent answering in purifying water body
It can be used for reducing ammonia nitrogen and nitrite in Aquatic farming animals water body with, lactobacillus plantarum CB5 or microbial bacterial agent
Content.
The content of present invention further includes a kind of Water body cleansing agent, and the cleanser includes the lactobacillus plantarum CB5 or institute
The microbial bacterial agent stated.
The utility model has the advantages that compared with prior art, the present invention has the advantage that the present invention is for the first time from Eriocheir sinensis enteron aisle
In isolated lactobacillus plantarum CB5, and it is aobvious to the bacteriostasis of aquatic pathogenic bacteria by verifying lactobacillus plantarum CB5
Write, and can be used for improving the immune function of Eriocheir sinensis, and significantly reduce in Aquatic farming animals water body ammonia nitrogen and
The content of nitrite has the function of purifying water body.
Detailed description of the invention
Fig. 1 is the phylogenetic tree that lactobacillus plantarum CB5 is constructed based on 16S rRNA gene order；
Fig. 2 is the pcr amplification product electrophoretogram of the 16S rRNA gene of lactobacillus plantarum CB5；Swimming lane 1:DL2000 DNA
Marker, swimming lane 2 and 3 are the pcr amplification product of the 16S rRNA gene of lactobacillus plantarum CB5.
Specific embodiment
Applicant will in conjunction with specific embodiments do specifically the preparation process of product of the present invention and application process below
It is bright, the present invention is expressly understood convenient for those skilled in the art.It is to be understood that following embodiment should not be solved in any way
It is interpreted as the claim of this application book being claimed the limitation of range.
Used MRS culture medium in the embodiment of the present invention, specific formula are following (g/L): peptone 10.0, beef extract
8.0, yeast extract 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.2, sulphur
Sour manganese 0.04, Tween 80 1.0.PH5.5~5.9,118 DEG C high pressure sterilization 15 minutes.
Triptic soya broth (TSA culture medium), specific formula are following (g/L): tryptone 17.0, soybean protein
Peptone 3.0, sodium chloride 5.0, dipotassium hydrogen phosphate 2.5, glucose 2.5.PH7.1~7.5,121 DEG C high pressure sterilization 15 minutes.
The Eriocheir sinensis mash feed that the present invention uses is purchased from Changzhou Jintan day and feed corporation,Ltd.
The separation and identification of 1 lactobacillus plantarum of embodiment
1.1 bacterial strains isolate and purify
It will be from the Eriocheir sinensis intestinal tissue homogenate sterile physiological of Jiangsu middle of the river source ecological aquaculture Co., Ltd
After salt water is diluted using 10 times of dilution methods, 10 are taken-5~10-8It is flat that the dilution of dilution range is coated on MRS solid medium
On plate.30 DEG C of constant temperature incubation 48h, picking typical case's single colonie, scribing line separation is to obtaining pure bacterial strain repeatedly.Using MRS culture medium from
A strains of lactic acid bacteria is separated in Eriocheir sinensis enteron aisle, for bacterium colony at the translucent protrusion of milky, neat in edge is smooth；Gram dye
Look mirror inspection is in Gram-positive, rod-shaped, has tart flavour, being numbered is CB5.
The identification of 1.2 Physiology and biochemistries
Bacterial strain colonial morphology is observed, Gram's staining, micro- sem observation.And the method that reference literature provides is to bacterium
Strain carry out catalase test, nitrate reduction test, gelatin liquefaction test, methyl red test (MR test), indole test,
Hydrogen sulfide production test, sugar fermentating test (micro fermentation tube method).
The Physiology and biochemistry qualification result of bacterial strain CB5 is shown in Table 1, preliminary to reflect with reference to " primary Jie Shi Bacteria Identification handbook " (the 8th edition)
Fixed its is lactobacillus plantarum (Lactobacillus plantarum).
The physio-biochemical characteristics of table 1 bacterial strain CB5 and reference culture
Table 1 The physiological and biochemical characteristics of CB5and
typical strain
Note :+indicate positive reaction ,-indicate negative reaction, (+) indicates torpid reaction.
Note: "+" .Positive reaction "-" .Negative reaction " (+) " .Torpid reaction
The PCR amplification and Phylogenetic Analysis of 1.3 16S rRNA genes
DNA, by specification operation are extracted using bacterial genomes DNA extraction kit.Using primer 2 7F (AGAGTTT-
GATCCTGGCTCAG) and the 16S rDNA gene of 1492R (TACCTTGTTACGACTT) amplification bacterium, amplification system reference are literary
Offer progress.Amplification program: enter PCR cycle after 95 DEG C of initial denaturation 5min, 95 DEG C of denaturation 30s, 50 DEG C of annealing 60s, 72 DEG C extend
30s, 40 circulations, 72 DEG C of extension 5min.PCR reaction system (50 μ L) are as follows: 10 × Taq buffer, 5 μ L, 1 μ L of template DNA,
Primer concentration is each 1 μ L of 10 μm of ol/L, 10 μm of 1 μ L, 5U/ μ L Taq DNA polymerases of ol/L dNTP 0.5 μ L, 40.5 μ of ultrapure water
L is mixed.1.0% agarose gel electrophoresis of amplified production simultaneously carries out recovery purifying, and primer synthesis and sequencing entrust Shanghai
Ying Jun biotechnology Co., Ltd completes.Sequence is submitted into GenBank database, searches for homologous sequence using BLASTN,
Homologous sequence is downloaded, application software Meg 4.0 carries out Phylogenetic Analysis, and BioEdit software analyzes identity, identifies bacterial strain.
16S rRNA segment is obtained through PCR amplification, is sequenced as 1448bp, the GenBank number of logging in is MK687387.Using
BLASTN is compared, and Clustal X constructs Neighbor-Joining tree, the result is shown in Figure 1.
As shown in Figure 1, the 16S rRNA of bacterial strain CB5 and known lactobacillus plantarum (L.plantarum) gather at one point
Zhi Shang, similitude 100%.Comprehensive morphological observation of characteristics, physiological and biochemical test, molecular biology sequencing result, from Sinensis
Isolated lactobacillus plantarum CB5 (L.plantarum CB5) in chela crab enteron aisle.
Lactobacillus plantarum CB5 is preserved in China typical culture collection center by us, and the deposit date is in April, 2019
8, deposit number was CCTCC NO；M 2019244.
Bacteriostasis of the 2 lactobacillus plantarum CB5 of embodiment to aquatic pathogenic bacteria
1, the preparation of cell free fermentation supernatant:
It after lactobacillus plantarum CB5 activation culture, is inoculated in MRS fluid nutrient medium with 4% inoculum concentration, 30 DEG C of standing trainings
It supports to stationary phase (36h~48h), 12000*g, 4 DEG C of centrifugation 15min, collection supernatant, 0.22 μm of membrane filtration of supernatant,
Thallus and other impurities are removed up to sterile fermented supernatant fluid.4 DEG C of refrigerators are set to save backup.
2, the bacteriostatic activity of aquatic pathogenic bacterium is tested:
This research is with aquatic pathogenic bacterium Hygrophilous monad TPS-30, BSK-10 of laboratory preservation, Eriocheir sinensis
Pathogenic aeromonas CL99909, staphylococcus aureus, vibrio parahaemolytious, Vibrio harveyi are strain subject, using agar
Beating holes method: pouring into the TSA culture medium (being suitable for tested bacterium growth) of 10ml heating and melting in sterilized petri dishes, sufficiently cold to it
But after solidifying, it is several to be put into sterilized Oxford cup.30mlTSA culture medium heating and melting is taken, 50 DEG C or so is cooled to it, connects
Enter the tested bacterium bacterium solution that 0.15 μ l is incubated overnight, is uniformly mixed rapidly, pours into plate.After cooling, aseptic nipper takes out Oxford cup.
The sterile fermented supernatant fluid that 200 μ l lactobacillus plantarum CB5 to be measured is drawn under sterile working is added in the cup aperture of Oxford, 30 DEG C of cultures
For 24 hours, antibacterial circle diameter (mm) is measured.
As shown in table 2, lactobacillus plantarum CB5 institute bacteriocinogeny to aquatic pathogenic bacterium Aeromonas TPS-30, BSK-10, in
The pathogenic aeromonas CL99909 of magnificent Eriocheir and staphylococcus aureus all have stronger bacteriostatic activity, to secondary haemolysis arc
The bacteriostatic activity of bacterium and Vibrio harveyi is weaker.
The antimicrobial spectrum of 2 lactobacillus plantarum CB5 institute bacteriocinogeny of table
Tab.2 Antibacterial spectrum of bacteriocin produced on Lactobacil1us
plantarum CB5
Effect of the 3 lactobacillus plantarum CB5 of embodiment to ammonia nitrogen and nitrite
1 materials and methods
1.1 lactobacillus plantarum
It after lactobacillus plantarum CB5 activation culture, is inoculated in MRS fluid nutrient medium with 4% inoculum concentration, 30 DEG C of standing trainings
It supports to stationary phase (36h~48h), with bacterial number in colony counting method measurement preparation when use, the bacterial number measured is 5 ×
109cfu/ml。
1.2 Eriocheir sinensis
Purchased from Huzhou City farm, weight is 50.2 ± 5g, is raised and train 1 week in cement pit with basal feed before experiment.
Effect of the 1.3 orally taken plant lactobacillus to ammonia nitrogen in water body and nitrite
1 × 10 is separately added into Eriocheir sinensis mash feed7、1×108、1×109With 2.5 × 109The plant of cfu/g
Object lactobacillus CB5,40 DEG C of drying.After Eriocheir sinensis adaptability cultivates 1 week, it is randomly divided into 5 groups and is placed in cultivation flume, water
25 ± 1 DEG C of temperature, each sink place 5, and each sink places Natural Water 50L and mesh sheet through being aerated, test 3 levels of setting
It repeats.Each sink is fed 1 time daily, feeds various concentration (1 × 10 every time7cfu/g、1×108cfu/g、1×109cfu/g、
2.5×109Cfu/g 1.5 grams of the feed of the CB5 containing lactobacillus plantarum), the Eriocheir sinensis powdery that control group feeds equivalent are raised
Material.Continuously feed 7 days.Feeding time be every morning 9:00-9:30, and in every afternoon 16:00 acquire in each sink
Water body, and measure the content of ammonia nitrogen and nitrite in water body.
1.4 mathematical statistics method
Test data is indicated with mean value ± standard error (Mean ± SE).The one-factor analysis of variance is carried out with SPSS11.5 software
(One-wayANOVA), to examine each index average value between different experiments group to whether there is significant difference.As there were significant differences
(P < 0.05) then makees the analysis of Duncan Multiple range test.
2. result
The content (mg/L) of 3 Nitrite of table
| Group | 1st day | 4th day | 7th day | 10th day |
| 1×107cfu/g | 0.045±0.006 | 0.106±0.019 | 0.277±0.067 | 0.521±0.063 |
| 1×108cfu/g | 0.044±0.004 | 0.132±0.024 | 0.250±0.023 | 0.487±0.078 |
| 1×109cfu/g | 0.045±0.008 | 0.118±0.034 | 0.238±0.052 | 0.365±0.065 |
| 2.5×109cfu/g | 0.043±0.002 | 0.120±0.028 | 0.201±0.045 | 0.329±0.033 |
| Control group | 0.045±0.001 | 0.125±0.021 | 0.311±0.038 | 0.674±0.049 |
As can be seen from Table 3, compared to the blank group, in 4d, each concentration group of adding to the changes of contents of nitrite,
There is reduction compared with the control group at the 7th day, wherein 2.5 × 109Cfu/g concentration group significantly reduces (P < 0.05), 1 ×
109The content of cfu/g concentration group nitrite reduces 23.5%.There is reduction compared with the control group at the 10th day, except 1 ×
107Outside cfu/g concentration group, remaining group significantly reduces (P < 0.05) compared with the control group.Although it can be seen that each concentration
The content of the nitrite of group is constantly in ascent stage, but its content can significantly reduce compared with the control, illustrate orally taken plant
Lactobacillus CB5 can reduce the content of Eriocheir sinensis Nitrite.
The content (mg/L) of ammonia nitrogen in 4 water body of table
As can be seen from Table 4, it is little that the changes of contents of concentration group compared with the control group is respectively added when 4d, at the 7th day with
Control group is compared to there is reduction, wherein 2.5 × 109Cfu/g concentration group significantly reduces (P < 0.05), and 109Concentration group ammonia nitrogen contains
Amount reduces 42.6%.There is reduction compared with the control group at the 10th day, removes 1 × 107Cfu/g and 1 × 108Cfu/g concentration group
Outside, remaining group significantly reduces (P < 0.05) compared with the control group.Meanwhile the although ammonia nitrogen of each concentration group as shown in Table 4
Content is constantly in ascent stage, but its content can significantly reduce compared with the control, and it is feeding to illustrate that orally taken plant lactobacillus can reduce
Grow the content of ammonia nitrogen in Eriocheir sinensis water body.After adding lactobacillus plantarum feeding Eriocheir sinensis in this possible feed,
Intestinal colonisation may generate the enzyme for accelerating feed decomposition and the utilization rate for improving feed, it is also possible to Activities of Some Plants cream bar
Bacterium is discharged into water body by aquatic livestock excrement, and excrement is accelerated to decompose, and reduces pollution of the excrement to water body, or be itself just
Have the function of degradation of ammonia nitrogen or nitrite.Therefore, the lactobacillus plantarum CB5 in the present invention can improve to a certain degree water body
Water quality, and not only reduce the working strength of raiser by oral way, has saved cost, and can with the utilization rate of feed,
With very big popularization and application foreground.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
Above preferred embodiment is crossed invention is described in detail, it is understood by those skilled in the art that it is possible to
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (9)
1. a lactobacillus plantarum CB5, which is characterized in that the lactobacillus plantarum CB5 is preserved in China typical culture collection
Center, the deposit date is on April 8th, 2019, deposit number was CCTCC NO:M 2019244.
2. a kind of microbial bacterial agent, which is characterized in that the microbial bacterial agent includes lactobacillus plantarum described in claim 1
CB5。
3. lactobacillus plantarum CB5 described in claim 1, microbial bacterial agent as claimed in claim 2 answering in aquaculture
With.
4. lactobacillus plantarum CB5 described in claim 1, microbial bacterial agent as claimed in claim 2 inhibit or kill in preparation
Application in aquatic pathogenic bacteria reagent or drug.
5. application according to claim 4, which is characterized in that the aquatic pathogenic bacteria includes Aeromonas hydrophila
The pathogenic aeromonas CL99909 of TPS-30, Aeromonas hydrophila BSK-10, Eriocheir sinensis, staphylococcus aureus, pair are molten
One or more of blood vibrios or Vibrio harveyi.
6. lactobacillus plantarum CB5 described in claim 1, microbial bacterial agent as claimed in claim 2 are preparing immunopotentiator
In application.
7. application according to claim 6, which is characterized in that the immunopotentiator includes the hair of lactobacillus plantarum CB5
Zymotic fluid.
8. lactobacillus plantarum CB5 described in claim 1, microbial bacterial agent as claimed in claim 2 answering in purifying water body
With.
9. a kind of Water body cleansing agent, which is characterized in that the cleanser include lactobacillus plantarum CB5 described in claim 1 or
Microbial bacterial agent as claimed in claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910374142.7A CN110004096A (en) | 2019-05-06 | 2019-05-06 | One lactobacillus plantarum and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910374142.7A CN110004096A (en) | 2019-05-06 | 2019-05-06 | One lactobacillus plantarum and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110004096A true CN110004096A (en) | 2019-07-12 |
Family
ID=67175915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910374142.7A Pending CN110004096A (en) | 2019-05-06 | 2019-05-06 | One lactobacillus plantarum and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110004096A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100833A (en) * | 2019-12-31 | 2020-05-05 | 浙江省淡水水产研究所 | Recombinant strain for expressing outer membrane protein of Edwardsiella ictaluri, preparation method and application |
| CN114657081A (en) * | 2020-12-22 | 2022-06-24 | 湖北绿天地生物科技有限公司 | Ternary composite bacterium preparation and application thereof |
| CN115948277A (en) * | 2022-09-14 | 2023-04-11 | 大连理工大学 | Lactobacillus plantarum with low-temperature tolerance and biocontrol antagonism characteristics and application thereof |
| CN116376776A (en) * | 2023-04-20 | 2023-07-04 | 湖南省水产科学研究所 | Compound microorganism for preventing and treating Sinilabeo Hunan dace bacterial enteritis and application thereof |
| CN117568240A (en) * | 2024-01-08 | 2024-02-20 | 中国水产科学研究院南海水产研究所 | Lactobacillus plantarum for inhibiting aeromonas hydrophila, and preparation method and application of cell-free supernatant of lactobacillus plantarum |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338198A (en) * | 2017-05-24 | 2017-11-10 | 浙江省淡水水产研究所 | A kind of Lactobacillus plantarum and its application |
-
2019
- 2019-05-06 CN CN201910374142.7A patent/CN110004096A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338198A (en) * | 2017-05-24 | 2017-11-10 | 浙江省淡水水产研究所 | A kind of Lactobacillus plantarum and its application |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100833A (en) * | 2019-12-31 | 2020-05-05 | 浙江省淡水水产研究所 | Recombinant strain for expressing outer membrane protein of Edwardsiella ictaluri, preparation method and application |
| CN114657081A (en) * | 2020-12-22 | 2022-06-24 | 湖北绿天地生物科技有限公司 | Ternary composite bacterium preparation and application thereof |
| CN115948277A (en) * | 2022-09-14 | 2023-04-11 | 大连理工大学 | Lactobacillus plantarum with low-temperature tolerance and biocontrol antagonism characteristics and application thereof |
| CN116376776A (en) * | 2023-04-20 | 2023-07-04 | 湖南省水产科学研究所 | Compound microorganism for preventing and treating Sinilabeo Hunan dace bacterial enteritis and application thereof |
| CN117568240A (en) * | 2024-01-08 | 2024-02-20 | 中国水产科学研究院南海水产研究所 | Lactobacillus plantarum for inhibiting aeromonas hydrophila, and preparation method and application of cell-free supernatant of lactobacillus plantarum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110004096A (en) | One lactobacillus plantarum and its application | |
| CN105238722B (en) | The preparation method and application of one bacillus amyloliquefaciens bacterial strain and its bacterium powder preparation | |
| CN105062921B (en) | It is a kind of inhibit fowl pathogenic salmonella Lactobacillus salivarius and its application | |
| CN107338198B (en) | Lactobacillus plantarum and application thereof | |
| CN108251335B (en) | Enterococcus faecalis HKF7 with lactic acid activity and screening culture method and application thereof | |
| CN109161509B (en) | Bacterial strain capable of preventing and treating bovine and sheep diarrhea | |
| CN105018379A (en) | Lactobacillus plantarum with high antioxidant activity and application of lactobacillus plantarum | |
| CN106754470A (en) | The Lactobacillus rhamnosus in one plant of lacto source and its application | |
| CN107267408A (en) | A kind of Lactobacillus salivarius JM55 and its application | |
| CN113040390B (en) | Probiotic salt-tolerant lactobacillus johnsonii and application thereof in preventing and treating pathogenic bacteria in livestock and poultry aquiculture | |
| CN112574924B (en) | Bacillus subtilis strain, microecological preparation and application thereof | |
| CN105524855B (en) | A kind of bacillus coagulans and its application with aquatic pathogenic bacterium antagonistic properties | |
| CN109321505A (en) | A kind of complex microorganism preparations adjusting aquatic livestock enteron aisle | |
| CN108004182A (en) | One plant of lactobacillus paracasei and its application in aquaculture | |
| CN114806978B (en) | Lactobacillus johnsonii SXDT-23 and application thereof | |
| CN112375712A (en) | Lactococcus lactis and application thereof | |
| CN103301409B (en) | Composite preventing and treating shrimp secretly-dying disease and preparation method for same | |
| CN115927102A (en) | Prisella megaterium, microbial inoculum, preparation method and application thereof | |
| CN102517227A (en) | Enterococcus faecalis and applications and feed additive and leavening agent thereof | |
| CN107937301B (en) | Bacillus amyloliquefaciens and application thereof in aquaculture | |
| CN110452849B (en) | Probiotic lactobacillus plantarum | |
| CN106701645A (en) | Bacillus amyloliquefaciens B7 with immune growth promoting effect and application method of bacillus amyloliquefaciens B7 | |
| CN106479915A (en) | A kind of preparation method and applications of Chalcalbumus chalcoides aralensis cultivation compound micro-ecological preparation | |
| CN112592851B (en) | Lactobacillus acidophilus with broad-spectrum antagonistic effect on aquatic pathogenic bacteria and application thereof | |
| Ebnetorab et al. | Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 212000 No. 268 Huayang Road, Dagang District, Zhenjiang, Jiangsu Applicant after: Jiangsu jiangxinyuan Ecological Agriculture Co.,Ltd. Applicant after: ZHEJIANG INSTITUTE OF FRESH WATER FISHERIES Address before: 212000 No. 268 Huayang Road, Dagang District, Zhenjiang, Jiangsu Applicant before: Jiangsu Jiangxinyuan Eco-aquatic Products Co.,Ltd. Applicant before: ZHEJIANG INSTITUTE OF FRESH WATER FISHERIES |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190712 |
|
| RJ01 | Rejection of invention patent application after publication |