CN110004070A - One plant of production zytase aspergillus niger and its construction method and application - Google Patents
One plant of production zytase aspergillus niger and its construction method and application Download PDFInfo
- Publication number
- CN110004070A CN110004070A CN201910284360.1A CN201910284360A CN110004070A CN 110004070 A CN110004070 A CN 110004070A CN 201910284360 A CN201910284360 A CN 201910284360A CN 110004070 A CN110004070 A CN 110004070A
- Authority
- CN
- China
- Prior art keywords
- aspergillus niger
- gene
- msb2
- xylan
- zytase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000228245 Aspergillus niger Species 0.000 title claims abstract description 48
- 238000010276 construction Methods 0.000 title claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 33
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 150000004823 xylans Chemical class 0.000 claims abstract description 30
- 229920001221 xylan Polymers 0.000 claims abstract description 29
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 239000002773 nucleotide Substances 0.000 claims abstract description 11
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
- 230000002779 inactivation Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 12
- 235000015099 wheat brans Nutrition 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 239000007836 KH2PO4 Substances 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 210000001938 protoplast Anatomy 0.000 claims description 7
- 238000003209 gene knockout Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 229920002635 polyurethane Polymers 0.000 claims description 4
- 239000004814 polyurethane Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 241000228212 Aspergillus Species 0.000 claims description 3
- 229910052564 epsomite Inorganic materials 0.000 claims description 3
- 230000006801 homologous recombination Effects 0.000 claims description 3
- 238000002744 homologous recombination Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000002657 fibrous material Substances 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 14
- 239000001963 growth medium Substances 0.000 description 14
- 239000007788 liquid Substances 0.000 description 12
- 239000002609 medium Substances 0.000 description 9
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 239000001965 potato dextrose agar Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 230000009182 swimming Effects 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 229960003487 xylose Drugs 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 150000004804 polysaccharides Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 241001370055 Aspergillus niger CBS 513.88 Species 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000032770 biofilm formation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 108010081495 driselase Proteins 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000019256 formaldehyde Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 108010087005 glusulase Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910284360.1A CN110004070B (en) | 2019-04-10 | 2019-04-10 | Xylanase-producing Aspergillus niger genetically engineered bacterium and construction method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910284360.1A CN110004070B (en) | 2019-04-10 | 2019-04-10 | Xylanase-producing Aspergillus niger genetically engineered bacterium and construction method and application thereof |
Publications (3)
Publication Number | Publication Date |
---|---|
CN110004070A true CN110004070A (en) | 2019-07-12 |
CN110004070A8 CN110004070A8 (en) | 2020-07-31 |
CN110004070B CN110004070B (en) | 2020-11-03 |
Family
ID=67170758
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910284360.1A Active CN110004070B (en) | 2019-04-10 | 2019-04-10 | Xylanase-producing Aspergillus niger genetically engineered bacterium and construction method and application thereof |
Country Status (1)
Country | Link |
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CN (1) | CN110004070B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111088173A (en) * | 2019-12-26 | 2020-05-01 | 南京高新工大生物技术研究院有限公司 | Aspergillus niger genetically engineered bacterium and construction method and application thereof |
CN112708567A (en) * | 2021-01-26 | 2021-04-27 | 天津科技大学 | Fructosyltransferase and high-yield strain thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103305428A (en) * | 2013-06-26 | 2013-09-18 | 武汉合缘绿色生物工程有限公司 | Aspergillus niger strain and application thereof |
CN103923840A (en) * | 2014-03-28 | 2014-07-16 | 中国科学院广州能源研究所 | Aspergillus niger for largely producing xylanase and application thereof |
CN103992954A (en) * | 2014-03-28 | 2014-08-20 | 中国科学院广州能源研究所 | High xylanase yield Aspergillus niger and application thereof |
CN106085881A (en) * | 2016-07-12 | 2016-11-09 | 西北农林科技大学 | A kind of Aspergillus niger strain and application thereof |
CN106381273A (en) * | 2015-07-29 | 2017-02-08 | 青岛蔚蓝生物集团有限公司 | Aspergillus niger strain highly producing xylanase |
-
2019
- 2019-04-10 CN CN201910284360.1A patent/CN110004070B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103305428A (en) * | 2013-06-26 | 2013-09-18 | 武汉合缘绿色生物工程有限公司 | Aspergillus niger strain and application thereof |
CN103923840A (en) * | 2014-03-28 | 2014-07-16 | 中国科学院广州能源研究所 | Aspergillus niger for largely producing xylanase and application thereof |
CN103992954A (en) * | 2014-03-28 | 2014-08-20 | 中国科学院广州能源研究所 | High xylanase yield Aspergillus niger and application thereof |
CN106381273A (en) * | 2015-07-29 | 2017-02-08 | 青岛蔚蓝生物集团有限公司 | Aspergillus niger strain highly producing xylanase |
CN106085881A (en) * | 2016-07-12 | 2016-11-09 | 西北农林科技大学 | A kind of Aspergillus niger strain and application thereof |
Non-Patent Citations (1)
Title |
---|
YASSER BAKRI等: "Xylanase Production by a Newly Isolated Aspergillus Niger SS7 in Submerged Culture", 《POL J MICROBIOL》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111088173A (en) * | 2019-12-26 | 2020-05-01 | 南京高新工大生物技术研究院有限公司 | Aspergillus niger genetically engineered bacterium and construction method and application thereof |
CN111088173B (en) * | 2019-12-26 | 2021-08-20 | 南京高新工大生物技术研究院有限公司 | Aspergillus niger genetically engineered bacterium and construction method and application thereof |
CN112708567A (en) * | 2021-01-26 | 2021-04-27 | 天津科技大学 | Fructosyltransferase and high-yield strain thereof |
Also Published As
Publication number | Publication date |
---|---|
CN110004070B (en) | 2020-11-03 |
CN110004070A8 (en) | 2020-07-31 |
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Correction item: Biological Conservation Information Correct: CCTCC|NO:M2019115|2019.02.28 Number: 28-02 Page: The title page Volume: 35 Correction item: Biological Conservation Information Correct: CCTCC|NO:M2019115|2019.02.28 Number: 28-02 Volume: 35 |
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Address after: 210000, 5 new model street, Gulou District, Jiangsu, Nanjing Applicant after: Nanjing Tech University Address before: 210000 No.30 Puzhu South Road, Pukou District, Nanjing City, Jiangsu Province, Nanjing Tech University Applicant before: Nanjing Tech University |
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