CN110004044A - A kind of Molecular Detection integration device and its detection method based on electrochemical sensing - Google Patents
A kind of Molecular Detection integration device and its detection method based on electrochemical sensing Download PDFInfo
- Publication number
- CN110004044A CN110004044A CN201910339391.2A CN201910339391A CN110004044A CN 110004044 A CN110004044 A CN 110004044A CN 201910339391 A CN201910339391 A CN 201910339391A CN 110004044 A CN110004044 A CN 110004044A
- Authority
- CN
- China
- Prior art keywords
- group
- chamber
- detection
- integration device
- electrochemical sensing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
Abstract
The invention discloses a kind of Molecular Detection integration device and its detection method based on electrochemical sensing, the detection method can complete Molecular Detection whole flow process in the integrated device of sealing, such as the extraction, amplification/hybridization and detection of nucleic acid.The Molecular Detection integration device based on electrochemical sensing is a totally-enclosed system, including reaction chamber group, reagent chamber's group, equal liquid chamber group, matched movable plunger and electrode detection room, the reaction process of Molecular Detection by movable plunger pull-up and under push away control, each independent sealed storage of reagent, it can guarantee that sample will not be obscured, it not will cause Aerosol Pollution simultaneously, can also reduce the probability that testing staff is infected by germ.The movable plunger of the Molecular Detection integration device based on electrochemical sensing can also be controlled by external impetus and realize automatic detection.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly, to a kind of Molecular Detection based on electrochemical sensing
Integrated device and its detection method.
Background technique
Nucleic acid method is applied molecular biology method, detects the variation of pathogenic bacteria inhereditary material structure or expression, after
And make the technology of diagnosis.Detection process includes Sample pretreatment and purifying, amplification/hybridize and detection etc..In the detection process,
All operation processings need to use cartridge, but the purifying of sample, amplification, hydridization and detection are all in different cartridges
Interior completion rarely has purifying, amplification, hydridization and detects comprehensive integrated cartridge.In traditional detection method, testing staff
Prepare various reagents, test tube it is generally necessary to take a long time, using a variety of instruments, personnel's operating process is complicated, automates journey
To spend not high, when pattern detection, is easy to appear cross contamination, a series of problems, such as false positive occurs in result, and sensing chamber's cartridge
Open environment easily causes operator to be infected by germ.
Summary of the invention
The technical problem to be solved by the present invention is in the prior art detection efficiency is low, accuracy is not high, ease for use not
Strong deficiency provides a kind of Molecular Detection integration device based on electrochemical sensing.
Another technical problem of the present invention is to provide to be examined using the Molecular Detection integration device based on electrochemical sensing
The method of survey.
The purpose of the present invention is achieved by the following technical programs:
A kind of Molecular Detection integration device based on electrochemical sensing, including reaction chamber group, reagent chamber's group, movable plunger and electricity
Pole sensing chamber group, the reaction chamber group and reagent chamber's group are the hollow cavity built in device, and the opening of cavity is in device surface, institute
It states movable plunger and the cavity of reaction chamber group and reagent chamber's group matches, movable plunger can pull up in the cavity by external impetus
It is pushed away under or, detecting electrode is equipped in the electrode detection room, detecting electrode can connecting detection instrument by conducting wire.The reaction chamber group
It is connect with reagent chamber group by pipeline, reaction chamber group is connect with detection group by pipeline, between the reaction chamber group and reagent chamber's group
Equipped with valve.
Further, equal liquid chamber group is additionally provided between the reaction chamber group and electrode detection room group, equal liquid chamber group is hollow
Cavity has movable plunger to match with it, and equal liquid chamber group bottom has pipeline to be correspondingly connected with sensing chamber group, and connecting pipe is equipped with stream
Body one-way cock.Preferably, the equal liquid chamber in the equal liquid chamber group is generally aligned in the same plane, by mutually level pipe between equal liquid chamber
Road connection, it is effective to guarantee that detecting indoor solution divides equally, and reduces detection error.It is further preferred that between the equal liquid chamber
Equipped with valve.
Further, the reaction chamber group includes at least 1 reaction chamber, and reagent chamber's group includes at least 1 solvent compartment,
The equal liquid chamber group includes at least 3 equal liquid chambers, and electrode detection room group includes at least 3 electrode detection rooms.Reaction chamber group
Molecule (nucleic acid or protein) different material reaction separately purification can be met.
Further, reaction chamber group bottom is equipped with magnet and heater.Magnetisable material can be adsorbed on by the magnet
Reaction chamber bottom can be adsorbed or react with target substance, be also possible to prevent magnetisable material and be lost or impact to detection.Add
Hot device can be adjusted reaction temperature.
Further, the pipeline junction of the connection reaction chamber is equipped with two-way fluid valve, the connection of reagent chamber's group pipeline
Mouth is equipped with two-way fluid valve or fluid non-return valve door, and the indoor reagent indentation reaction chamber reaction of reagent, can press after reaction can original
Solvent storage room.Fluid non-return valve door is equipped between the equal liquid chamber and electrode detection room.
Further, the Molecular Detection integration device based on electrochemical sensing further includes waste liquid chamber, the waste liquid
Room is connect with reaction chamber group by pipeline, and fluid non-return valve door is equipped between the waste liquid chamber and reaction chamber group.It is useless after having reacted
Liquid is discharged directly into waste liquid chamber, it is ensured that the former indoor purity of solvent is used multiple times.
Further, the external impetus is provided by one or more modes of motor, air pump, liquid pump.Micro- place can be passed through
Control of the device to external impetus is managed, automatic detection is realized, solves the problems, such as that personnel infect because of tested for pathogens.
Further, the detecting electrode is two and more than two electrodes.Preferably, the electrode detection system is
Three electrodes, including working electrode, reference electrode and auxiliary electrode.
Further, the Molecular Detection integration device based on electrochemical sensing can be used for the purifying and detection of molecule
It is one or more.Preferably, the Molecular Detection integration device based on electrochemical sensing can be used for the purification of nucleic acid, expand
Increase, hybridize and detects one or more.The Molecular Detection integration device based on electrochemical sensing can also be used for albumen
Matter is purified and is detected one or more.
According to the above-mentioned Molecular Detection integration device based on electrochemical sensing, provide a kind of using based on electrochemical sensing
The method that is detected of Molecular Detection integration device, step includes:
S1. the liquid or solids such as required reagent, magnetisable material and electrolyte substance is sealed up for safekeeping in advance respectively into reagent chamber's group, activity
Plunger seals, then detected material is put into reaction chamber, beyond the Great Wall movable plunger.
S2. by reagent set reaction reagent and magnetisable material respectively by movable plunger be pressed into reaction chamber in be detected
Object, heating mix, and are pushed away under by the pull-up of movable plunger in reaction chamber and the movable plunger of solvent compartment and realize the mixed of solution
It is even.
S3. after having reacted, detected material is adsorbed on by reaction chamber by the magnetisable material of reaction chamber bottom, by reaction reagent
Former solvent storage chamber or waste liquid chamber are pressed by movable plunger.
S4. it repeats step S2 ~ 3 to operate each solvent indentation reaction chamber, the one kind purify nucleic acid, expand, hybridizing
Or it is a variety of, after mixing with solvent by detected material, mixed solution is pressed into the equal uniform liquid separation of liquid chamber group.
S5. the mixed liquor in liquid separation room is pressed into corresponding electrode detection room, will test electrode and inspection with conducting wire
Instrument is surveyed to connect and detect mixing liquid.
Compared with prior art, beneficial effect is:
The invention in a device complete Molecular Detection whole flow process, such as nucleic acid and protein extraction,
Amplification/hybridization and detection.One totally-enclosed system of Molecular Detection integration device of the present invention, required reagent is sealed in advance
There are in solvent compartment's group in device, by the pull-up of movable plunger and under push away and realize the mixing and separation of solvent, guarantee tested
Sample will not be obscured, while not will cause Aerosol Pollution, reduce the probability that testing staff is infected by germ nucleic acid.Institute of the present invention
It is simple to state Molecular Detection integration device architecture, being able to solve Pathogen detection, there are caused by cross contamination, human operational error
The not high problem of accuracy.
Sample will be added in the reaction chamber group in device manually for the method for the invention, can pass through external impetus pair
The mixing of the elevating control solvent and sample of mobile plunger, and can be realized by external impetus processor program to germ
The automatic detection that nucleic acid or protein extracts, amplification/hybridization, equal liquid are quantitative.
Detailed description of the invention
Fig. 1 is Molecular Detection integration device surface structure chart of the present invention；
Fig. 2 is Molecular Detection integration bottom device structure chart of the present invention；
Fig. 3 is Molecular Detection integration device junction composition of the present invention；
Fig. 4 is the equal liquid chamber of Molecular Detection integration device of the present invention and electrode detection room connection figure.
Wherein, 1, reaction chamber；2, reagent chamber；3, equal liquid chamber；4, electrode detection room；5 two-way fluid valves；6, fluid is unidirectional
Valve；7, magnet；8, heater；9, movable plunger.
Specific embodiment
It is explained further and illustrates below with reference to embodiment, but specific embodiment does not have any type of limit to the present invention
It is fixed.Unless otherwise specified, method and apparatus used in embodiment is ability conventional method and equipment, raw materials used is routine
Marketable material.
Embodiment 1
As shown in Fig. 1 ~ 3, integrated cartridge, including 3, the five electrode inspections of the equal liquid chamber of a reagent chamber 2, five of reaction chamber 1, six
Room 4 and movable plunger 9 are surveyed, the reaction chamber 1, reagent chamber 2 and equal liquid chamber 3 are the hollow cavity built in cartridge, the opening of cavity
On cartridge surface, the cavity of the movable plunger 9 and reaction chamber 1, reagent chamber 2 and liquid chamber 3 matches, and movable plunger 9 can be borrowed
Help external impetus pull up in the cavity or under push away.Working electrode, reference electrode and auxiliary electricity are installed in the electrode detection room 4
Pole, detecting electrode can connecting detection instrument by conducting wire.The reaction chamber 4 is connect with reagent chamber 2 by pipeline, simultaneous reactions room 1
By pipeline, one of those is connect with equal liquid chamber 3.Five equal liquid chambers 3 are connected by the pipeline of identical height level, equal liquid chamber 3 with
Electrode detection room 4 is correspondingly connected in bottom by pipeline.It is double that fluid is equipped in reaction chamber 1 and the entrance of each reagent chamber 2
To valve 5, fluid non-return valve door 6 is equipped between the electrode detection room 4 of the corresponding connection of equal liquid chamber 3.
1 bottom of reaction chamber is equipped with magnet 7 and heater 8, and magnetisable material can be adsorbed on reaction chamber 1 by the magnet 7
Bottom prevents magnetisable material to be lost or impact to detection.The heater 8 of bottom can carry out temperature tune to reaction dissolvent
Section.
The external impetus is provided by one or more modes of motor, air pump, liquid pump.It can be by microprocessor to outside
Nucleic acid automatic detection is realized in the control of power.
Embodiment 2
A kind of plunger type detection of nucleic acids integration cartridge, including a waste liquid chamber, two reaction chambers, six reagent chamber, three
Liquid chamber, three sensing chamber and movable plunger, the reaction chamber, reagent chamber and equal liquid chamber are the hollow cavity built in cartridge, cavity
Opening in cartridge surface, the movable plunger and reaction chamber group, reagent chamber's group, the cavity phase of equal liquid chamber group and sensing chamber's group
Match, movable plunger can be pulled up in the cavity by external impetus or under push away.The electrode detection indoor location has working electrode, ginseng
Than electrode and auxiliary electrode, detecting electrode can connecting detection instrument by conducting wire.The reaction chamber is connect with waste liquid chamber, reaction chamber with
Reagent chamber's group is connected by pipeline, and by pipeline, one of those is connect with equal liquid chamber for simultaneous reactions room.Five equal liquid chambers are by phase
The pipeline of same level height connects, and equal liquid chamber is correspondingly connected in bottom by pipeline with electrode detection room.In the nozzle of reaction chamber
Equipped with two-way fluid valve, the entrance of each reagent chamber is equipped with fluid non-return valve door, is equipped with valve, equal liquid between equal liquid chamber
It is equipped with fluid non-return valve door between the electrode detection room of the corresponding connection in room, it is unidirectional that fluid is equipped between reaction chamber and waste liquid chamber
Valve.
The reaction chamber bottom is equipped with magnet and heater, and magnetisable material can be adsorbed on reaction chamber bottom by the magnet,
Magnetisable material is prevented to be lost or impact to detection.The heater of bottom can carry out temperature adjusting to reaction dissolvent.
The external impetus is provided by one or more modes of motor, air pump, liquid pump.It can be by microprocessor to outside
Nucleic acid automatic detection is realized in the control of power.
Above-described embodiment only to clearly illustrate example of the present invention, and is not to embodiments of the present invention
Restriction.For those of ordinary skill in the art, on the basis of the above description can also according to the needs of detection,
The adjustment of number, volume size and location relationship is carried out to reaction chamber, reagent chamber, equal liquid chamber and electrode detection room.
Embodiment 3
This implementation provides the detection method of plunger type detection of nucleic acids integration cartridge described in embodiment, and step includes:
S1. reagent, magnetisable material needed for detection of nucleic acids and electrolyte are sealed up for safekeeping in advance into reagent chamber's group, and detected material is put into reaction
In room, movable plunger beyond the Great Wall；
S2. by reagent set reaction reagent and magnetisable material respectively by movable plunger be pressed into reaction chamber in detected material,
Heating reaction, after having reacted, is adsorbed on reaction chamber for detected material by the magnetisable material of reaction chamber bottom, reaction reagent is led to
It crosses movable plunger and is pressed into former solvent storage chamber or waste liquid chamber；
S3. step S2 is repeated by the indentation reaction chamber operation of each solvent, what is purified, expanded to nucleic acid, hybridized is one or more,
Solution after mixing, is pressed into the equal uniform liquid separation of liquid chamber group by detected material and electrolyte；
S4. the mixed liquor in liquid separation room is pressed into corresponding electrode detection room, will test electrode and detector with conducting wire
It connects and mixing liquid is detected.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.There is no necessity and possibility to exhaust all the enbodiments.It is all this
Made any modifications, equivalent replacements, and improvements etc., should be included in the claims in the present invention within the spirit and principle of invention
Protection scope within.
Claims (10)
1. a kind of Molecular Detection integration device based on electrochemical sensing, which is characterized in that described based on electrochemical sensing
Molecular Detection integration device includes reaction chamber group, reagent chamber's group, movable plunger and electrode detection room group, the reaction chamber group and
Reagent chamber's group is the hollow cavity built in device, and the opening of cavity is in device surface, the movable plunger and reaction chamber group and examination
The cavity of agent room group matches, movable plunger can be pulled up in the cavity by external impetus or under push away, in the electrode detection room
Equipped with detecting electrode, detecting electrode can connecting detection instrument by conducting wire；The reaction chamber group is connect with reagent chamber group by pipeline,
Reaction chamber group is connect with electrode detection room group by pipeline；Valve is equipped between the reaction chamber group and reagent chamber's group.
2. the Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that the reaction
Equal liquid chamber group is additionally provided between room group and electrode detection room group, equal liquid chamber group is hollow cavity, there is movable plunger to match with it,
Equal liquid chamber group bottom has pipeline and sensing chamber's group to be correspondingly connected with, and connecting pipe is equipped with fluid non-return valve door；In the equal liquid chamber group
Equal liquid chamber be generally aligned in the same plane, between equal liquid chamber by mutually level pipeline connect；Valve is equipped between the equal liquid chamber.
3. the Molecular Detection integration device based on electrochemical sensing according to claim 2, which is characterized in that the reaction
Room group includes at least 1 reaction chamber；Reagent chamber's group includes at least 1 reagent chamber；The equal liquid chamber group includes at least 2
Liquid chamber；Electrode detection room group includes at least 2 electrode detection rooms.
4. the Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that the reaction
Room group bottom is equipped with magnet and heater.
5. any Molecular Detection integration device based on electrochemical sensing according to claim 1 ~ 3, which is characterized in that institute
The pipeline junction for stating connection reaction chamber is equipped with two-way fluid valve；Reagent chamber's group pipeline connection port is equipped with two-way fluid valve
Door or fluid non-return valve door.
6. the Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that the outside
Power is provided by one or more modes of motor, air pump, liquid pump.
7. the Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that the detection
Electrode is more than two electrodes；The electrode detection system is three electrodes, including working electrode, reference electrode and auxiliary electrode.
8. the Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that the plunger
Formula Molecular Detection integration cartridge further includes waste liquid chamber, and the waste liquid chamber is connect with reaction chamber group by pipeline；The waste liquid chamber
Fluid non-return valve door is equipped between reaction chamber group.
9. any Molecular Detection integration device based on electrochemical sensing according to claim 1 ~ 8, which is characterized in that institute
State the Molecular Detection integration device based on electrochemical sensing can be used for molecule purifying and detection it is one or more；The base
It can be used for the one or more of purification, amplification, hybridization and the detection of nucleic acid in the Molecular Detection integration device of electrochemical sensing；
The Molecular Detection integration device based on electrochemical sensing can also be used for the one or more of protein purification and detection.
10. -9 any Molecular Detection integration device based on electrochemical sensing according to claim 1, which is characterized in that
Using electrochemical sensing Molecular Detection integration device carry out Molecular Detection the step of include:
S1. the liquid or solids such as required reagent, magnetisable material and electrolyte substance is sealed up for safekeeping in advance respectively into reagent chamber's group, activity
Plunger seals, then detected material is put into reaction chamber, beyond the Great Wall movable plunger；
S2. by reagent set reaction reagent and magnetisable material respectively by movable plunger be pressed into reaction chamber in detected material,
Heating mixes, and pushes away the mixing for realizing solution under by the pull-up of movable plunger in reaction chamber and the movable plunger of solvent compartment；
S3. after having reacted, detected material is adsorbed on by reaction chamber by the magnetisable material of reaction chamber bottom, reaction reagent is passed through
Movable plunger is pressed into former solvent storage chamber or waste liquid chamber；
S4. step S2 ~ 3 are repeated, by the indentation reaction chamber operation of each solvent, one kind or more that nucleic acid is purified, is expanded, is hybridized
Kind, after mixing with electrolyte by detected material, mixed solution is pressed into the equal uniform liquid separation of liquid chamber group；
S5. the mixed liquor in liquid separation room is pressed into corresponding electrode detection room, will test electrode and detector with conducting wire
It connects and mixing liquid is detected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339391.2A CN110004044A (en) | 2019-04-25 | 2019-04-25 | A kind of Molecular Detection integration device and its detection method based on electrochemical sensing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339391.2A CN110004044A (en) | 2019-04-25 | 2019-04-25 | A kind of Molecular Detection integration device and its detection method based on electrochemical sensing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110004044A true CN110004044A (en) | 2019-07-12 |
Family
ID=67174208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910339391.2A Pending CN110004044A (en) | 2019-04-25 | 2019-04-25 | A kind of Molecular Detection integration device and its detection method based on electrochemical sensing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110004044A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
| CN113913431A (en) * | 2021-09-09 | 2022-01-11 | 湖南工业大学 | Phoxim aptamer, aptamer derivative and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006071770A2 (en) * | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| CN102620959A (en) * | 2002-12-26 | 2012-08-01 | 梅索磅秤技术有限公司 | Assay cartridges and methods of using the same |
| WO2015179712A1 (en) * | 2014-05-22 | 2015-11-26 | University Of Notre Dame Du Lac | Integrated membrane sensor for rapid molecular detection |
| CN105828945A (en) * | 2013-11-29 | 2016-08-03 | 基因波公司 | Microfluidic cartridge for molecular diagnosis, docking station using a microfluidic cartridge, and process for analyzing a biological sample |
| CN107855142A (en) * | 2017-11-01 | 2018-03-30 | 深圳市第二人民医院 | A kind of detection chip and detection device based on microflow control technique |
| CN109030369A (en) * | 2018-09-08 | 2018-12-18 | 重庆科技学院 | Multichannel micro-fluidic chip associated with a kind of and cuvette |
| CN109416365A (en) * | 2016-04-13 | 2019-03-01 | 深圳源光科技有限公司 | Multi-functional microfluidic device for biological sample screening |
| CN109488787A (en) * | 2018-10-31 | 2019-03-19 | 深圳市尚维高科有限公司 | Fluid containment structure and micro-fluidic chip with the structure and its operating method |
| CN208684959U (en) * | 2018-05-24 | 2019-04-02 | 杭州霆科生物科技有限公司 | A kind of nucleic acid amplification detection micro-fluidic chip |
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
-
2019
- 2019-04-25 CN CN201910339391.2A patent/CN110004044A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102620959A (en) * | 2002-12-26 | 2012-08-01 | 梅索磅秤技术有限公司 | Assay cartridges and methods of using the same |
| WO2006071770A2 (en) * | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| CN105828945A (en) * | 2013-11-29 | 2016-08-03 | 基因波公司 | Microfluidic cartridge for molecular diagnosis, docking station using a microfluidic cartridge, and process for analyzing a biological sample |
| WO2015179712A1 (en) * | 2014-05-22 | 2015-11-26 | University Of Notre Dame Du Lac | Integrated membrane sensor for rapid molecular detection |
| CN109416365A (en) * | 2016-04-13 | 2019-03-01 | 深圳源光科技有限公司 | Multi-functional microfluidic device for biological sample screening |
| CN107855142A (en) * | 2017-11-01 | 2018-03-30 | 深圳市第二人民医院 | A kind of detection chip and detection device based on microflow control technique |
| CN208684959U (en) * | 2018-05-24 | 2019-04-02 | 杭州霆科生物科技有限公司 | A kind of nucleic acid amplification detection micro-fluidic chip |
| CN109030369A (en) * | 2018-09-08 | 2018-12-18 | 重庆科技学院 | Multichannel micro-fluidic chip associated with a kind of and cuvette |
| CN109488787A (en) * | 2018-10-31 | 2019-03-19 | 深圳市尚维高科有限公司 | Fluid containment structure and micro-fluidic chip with the structure and its operating method |
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
| CN113913431A (en) * | 2021-09-09 | 2022-01-11 | 湖南工业大学 | Phoxim aptamer, aptamer derivative and application thereof |
| CN113913431B (en) * | 2021-09-09 | 2023-10-24 | 湖南工业大学 | Phoxim aptamer, aptamer derivative and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110331089B (en) | Full-automatic nucleic acid extraction amplification detection micro-fluidic chip box and application thereof | |
| CN111647498B (en) | Integrated self-service nucleic acid detection device and use method thereof | |
| CN109929735A (en) | A kind of plunger type detection of nucleic acids integration cartridge and its detection method | |
| WO2021237396A1 (en) | Integrated self-service nucleic acid detection device and use method thereor | |
| CN107099445B (en) | Test cartridge with integrated transport module | |
| CN102671729B (en) | Micro-fluidic chip for multi-index biochemical detection | |
| CN207933420U (en) | A kind of micro-fluidic chip of genetic test | |
| CN206375900U (en) | A kind of miniflow bag for nucleic acid integration detection | |
| CN110004044A (en) | A kind of Molecular Detection integration device and its detection method based on electrochemical sensing | |
| CN111500675A (en) | One-stop full-automatic closed nucleic acid extraction and real-time fluorescence PCR test combined kit | |
| CN107199061A (en) | A kind of multitask full-automatic biochemical detection chip | |
| WO2021254519A1 (en) | Sample processing and detection apparatus and application thereof | |
| US20210123903A1 (en) | Micro-fluidic Chip and Analytical Instrument Provided with the Micro-fluidic Chip | |
| JPH10337173A (en) | Microreactor for biochemical reaction | |
| CN106226254A (en) | A kind of micro-fluidic chip for biological detection and preparation method thereof | |
| EP1511836A1 (en) | Kit and process for microbiological for on-site examination of a liquid sample | |
| CN101410699A (en) | Fluid processing and volume determination system | |
| CN111638282A (en) | Portable detection equipment and detection method for rapid screening of liver diseases | |
| CN107964504A (en) | A kind of closed sample integrity detection device and detection method | |
| CN212453801U (en) | Integrated mobile pathogen nucleic acid detection laboratory system | |
| CN110055354B (en) | Nucleic acid composition, detection unit, microfluidic chip and detection device | |
| CN212293607U (en) | One-stop full-automatic closed nucleic acid extraction and real-time fluorescence PCR test combined kit | |
| CN211035866U (en) | Plunger type nucleic acid detection integrated card box | |
| CN115141739A (en) | Air microorganism sampling and detection integrated device and sampling and detection method | |
| Ji et al. | Study on the air-tightness detection system for pipetting in the automated aptamer selection instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |