CN109998601A - A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue - Google Patents
A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue Download PDFInfo
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- CN109998601A CN109998601A CN201910339466.7A CN201910339466A CN109998601A CN 109998601 A CN109998601 A CN 109998601A CN 201910339466 A CN201910339466 A CN 201910339466A CN 109998601 A CN109998601 A CN 109998601A
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- Prior art keywords
- runner
- umbilical cord
- fixed
- wheel body
- liquid groove
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 45
- 239000003292 glue Substances 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 230000003139 buffering effect Effects 0.000 claims abstract description 31
- 235000020637 scallop Nutrition 0.000 claims abstract description 18
- 241000237509 Patinopecten sp. Species 0.000 claims abstract description 17
- 210000001367 artery Anatomy 0.000 claims abstract description 5
- 210000003462 vein Anatomy 0.000 claims abstract description 4
- 239000000872 buffer Substances 0.000 claims description 11
- 238000003466 welding Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 11
- 210000001519 tissue Anatomy 0.000 description 20
- 210000000130 stem cell Anatomy 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 3
- 238000001990 intravenous administration Methods 0.000 description 3
- 210000003606 umbilical vein Anatomy 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000008274 jelly Substances 0.000 description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000237503 Pectinidae Species 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001644 umbilical artery Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0233—Pointed or sharp biopsy instruments
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medical Informatics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Prostheses (AREA)
Abstract
The present invention relates to field of medical auxiliary equipment, specifically a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue.Including buffering liquid groove, preceding runner, rear runner and fixed pin；Outside on the two end plates of buffering liquid groove positioned at centre bore is fixed with runner positioning plate, and runner positioning plate offers scallop respectively；Preceding runner and rear runner include the wheel body of tooth form and are fixed on wheel body and opposite to each other in the handle of buffering liquid groove side；Fixed pin includes a radicular vein needle and two radicular arteries needles, run through on the wheel body of preceding runner and offers the location hole being each passed through for three fixed pins, and three location holes are distributed on the wheel body of preceding runner in isosceles triangle, it is spaced apart on the wheel body of rear runner and is equipped with multiple matching holes, to be plugged in three fixed pins on rear runner by suitable matching hole according to the trend of umbilical cord medium vessels.The present invention can not only simplify the strip operation of magnificent Tong Shi glue, but also can reduce the risk that umbilical cord tissue pollutes in stripping process.
Description
Technical field
The present invention relates to field of medical auxiliary equipment, specifically a kind of auxiliary for people's umbilical cord periphery China Tong Shi glue
Stripping off device.
Background technique
Mescenchymal stem cell (MSC, mesenchymal stem cells) is the important member of stem cell line, source
In the mesoderm of mesoderm growing early stage, belong to multipotential stem cell, because it with multi-lineage potential, hematopoiesis support and promotes stem cell to plant
Enter, immunoregulation and be increasingly subject to the concern of people the features such as self-replacation.The main of human mesenchymal stem cell is obtained at present
Method is: the magnificent Tong Shi glue by the methods of shredding, tearing removing human umbilical tissue carries out paste block culture.
Magnificent Tong Shi glue (Wharton's Jelly) refers to the gel filler between umbilical cord amniotic membrane and blood vessel, logical in China
In the conventional stripping process of family name's glue, on the one hand, the surface of magnificent Tong Shi glue is smooth, structure consolidation, therefore shred/tear
There is larger difficulty when operation, brings many burdens to experimenter；Umbilical cord tissue is completely exposed to when on the other hand, due to operation
In air, pollution risk is increased, while excessive tissue drying also reduces the yield of mescenchymal stem cell, influences follow-up test
Progress.
Summary of the invention
The present invention is intended to provide a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue, can both simplify magnificent Tong Shi
The strip operation of glue, and the risk that umbilical cord tissue pollutes in stripping process can be reduced.
In order to solve the above technical problems, the technical solution adopted by the present invention are as follows: one kind is used for people's umbilical cord periphery China Tong Shi
The up-stripping device of glue including buffering liquid groove, the preceding runner that is rotatably arranged on buffering liquid groove one end, is rotatably arranged on buffer
It the rear runner of the slot other end and is plugged between preceding runner and rear runner and for umbilical cord to be fixed on buffering liquid groove position
Fixed pin；Buffering liquid groove includes bottom plate and the two blocks of side plates and two end plates that are fixed on bottom plate, is opened on two end plates
Equipped with centre bore, and the outside on two end plates positioned at centre bore is fixed with runner positioning plate, on runner positioning plate with
The corresponding position of the centre bore of end plate offers the scallop being coupled for preceding runner or rear runner respectively；Preceding runner is with after
Runner includes the wheel body of tooth form and is fixed on wheel body and opposite to each other in the handle of buffering liquid groove side；Fixed pin includes one quiet
Arteries and veins needle and two radicular arteries needles run through on the wheel body of preceding runner and offer the location hole being each passed through for three fixed pins, and three
A location hole is distributed on the wheel body of preceding runner in isosceles triangle, is spaced apart on the wheel body of rear runner and is equipped with multiple matching holes, with
Three fixed pins are plugged on rear runner by suitable matching hole according to the trend of umbilical cord medium vessels.
Preferably, the shape of scallop and wheel body is mutual corresponding six gear shape.
Preferably, two pieces of runner positioning plates are fixed on the two end plates of buffering liquid groove with welding manner respectively.
Preferably, centre bore and scallop are concentric, and the aperture of centre bore is greater than scallop.
Preferably, three location holes on the wheel body of preceding runner are evenly spaced in the periphery of the handle of preceding runner.
Preferably, the height of two blocks of side plates is less than the height of two end plates, so that the space trigone that three fixed pins surround
The half height of column is located under the liquid level for completely filling buffer in buffering liquid groove.
Beneficial effect
After fixed pin in through the invention is each passed through arteria umbilicalis and umbilical vein on umbilical cord tissue, by the both ends of fixed pin point
In the matching hole for not plugging the location hole for being fixed on preceding runner and rear runner, then preceding runner and rear runner are distinguished into location fit and pacified
After on the runner positioning plate being located on two end plates of buffering liquid groove, the fixation of umbilical cord tissue can be completed.Umbilical cord quilt at this time
It is stable to be fixed on device, it will not be flipped, slip because of subsequent operation, greatly reduce emergency risk and operation difficulty.
After umbilical cord is fixed, on umbilical cord more than the liquid level that operating area is located at buffering liquid groove, and umbilical cord lower edge is non-
Operating area is located at the liquid level of buffer once, and the not operation face of stripping process was both reduced not under buffer liquid level at this time
Tissue is exposed to bring pollution risk in air, in turn avoids that tissue is dry and cellular damage institute when excessively tearing tissue
The low problem of caused cell output capacity.
Detailed description of the invention
Fig. 1 is schematic perspective view of the invention；
Fig. 2 is the longitudinal profile structure schematic at main view visual angle of the present invention；
Marked in the figure: 1, rear runner, 2, centre bore, 3, location hole, 4, wheel body, 5, handle, 6, preceding runner, 7, runner positioning plate,
8, scallop, 9, bottom plate, 10, side plate, 11, end plate, 12, buffering liquid groove, 13, matching hole, 14, intravenous needle, 15, arterial needle, 16,
Fixed pin.
Specific embodiment
As shown in Figures 1 and 2, a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue of the invention, including
For holding the buffering liquid groove 12 of buffer, the fixed pin 16 for fixing umbilical cord, supporting fixed pin 16 for fixed, so that solid
Determine preceding runner 6 and rear runner 1 that the umbilical on needle 16 point is soaked in buffer.Preceding runner 6 and rear runner 1 separate respectively
The both ends of buffering liquid groove 12 are rotatably arranged on, so that circumferential each section of umbilical cord can be in preceding runner 6 and rear turn in fixed pin 16
Buffer liquid level or more is exposed under the rotary action of wheel 1, the strip operation of China's Tong Shi glue is carried out for experimenter, or makes to fix
The non-peel-away operating area of umbilical cord is soaked in buffer on needle 16, avoid pollution with it is over-drying.
The rectangular channel of the substantially open top of buffering liquid groove 12 including bottom plate 9 and is fixed on two pieces of sides of 9 two sides of bottom plate
Plate 10 and the two end plates 11 for being fixed on 9 both ends of bottom plate.Circular centre bore 2, centre bore 2 are offered on two end plates 11
Aperture be 18mm.The outside of centre bore 2, which is respectively welded, on two end plates 11 is equipped with for positioning fixed preceding runner 6 or rear turn
The runner positioning plate 7 of wheel 1, runner positioning plate 7 is circle, diameter 30mm.It is offered on runner positioning plate 7 and centre bore 2
Corresponding six concentric gear shape scallops 8, scallop 8 are diametrically 20mm outside, are inside diametrically 14mm.Preceding runner 6 is with after
Runner 1 includes the wheel body 4 of six gear shapes and is fixed on wheel body 4 opposite to each other in the handle 5 of 12 side center of buffering liquid groove.
The specification of wheel body 4 is corresponding with scallop 8, can plug and be fixed in scallop 8.In order to avoid slow in buffering liquid groove 12
Fliud flushing is oozed out by the gap between scallop 8 and wheel body 4, one layer of elastic jelly can be laid in the outer rim of wheel body 4, so that wheel body
Interference fit is formed between 4 and scallop 8, the connection also strengthened between wheel body 4 and runner positioning plate 7 while sealing is strong
Degree avoids the runner in tissue stripping process from loosening.
Fixed pin 16 includes an intravenous needle 14 and two arterial needles 15 labeled as red labeled as black.In forward
Run through on the wheel body 4 of wheel 6 and offer the location hole 3 being each passed through for three fixed pins 16, and three location holes 3 are in preceding runner 6
Wheel body 4 on centered on handle 5 in isosceles triangle be distributed.It is spaced apart on the wheel body 4 of rear runner 1 and is equipped with multiple matching holes 13,
Three fixed pins 16 to be plugged on rear runner 1 by suitable matching hole 13 according to the trend of umbilical cord medium vessels.This implementation
The height of two blocks of side plates 10 in example is less than the height of two end plates 11, so that the space triangular prism that three fixed pins 16 surround
Half height is located under the liquid level for completely filling buffer in buffering liquid groove 12.
Concrete application of the invention is as follows:
1), the buffering liquid groove 12 for being fixed with runner positioning plate 7 is placed on superclean bench；
2), fresh umbilical cord tissue is cleaned, after removing crimson blood, takes a black intravenous needle 14 and two red arterial needles 15,
It is each passed through the umbilical vein and two arteria umbilicalis of umbilical cord, to guarantee that umbilical cord China Tong Shi glue tissue will not be damaged.Fixed pin 16 is same
In location hole 3 before the end of side is individually fixed on runner 6, the handle 5 of runner 6, will be installed with the fixed pin of umbilical cord before holding
16 sequentially pass through the centre bore 2 and buffering liquid groove 12 in Fig. 1 on the end plate 11 of left side, until reaching the centre bore 2 of right side end plate 11
Position, and preceding runner 6 is fitted in the scallop 8 on left side runner positioning plate 7；
3) rear runner 1, is taken to be coupled in the scallop 8 on right side runner positioning plate 7, according to fixed pin 16 walk will definitely three it is solid
The free-end for determining needle 16 is fixed in the matching hole 13 that position is suitable on rear runner 1, and the fixed of umbilical cord can be completed.At this time
Umbilical cord is fixed on device by stable, will not be flipped, be slipped because of subsequent operation, greatly reduce emergency risk and operation
Difficulty；
4) PBS buffer solution, is added in 12 inside groove of buffering liquid groove, the umbilical cord part fixed is made to submerge buffer liquid level, it can be right
It is exposed to the umbilical cord part except liquid level and carries out China's Tong Shi glue strip operation, the not operation face of stripping process is not slow in PBS at this time
Under fliud flushing liquid level, tissue was both reduced and has been exposed to bring pollution risk in air, it is dry and excessive in turn avoid tissue
The low problem of cell output capacity caused by cellular damage when tearing tissue；
5), after the completion of the umbilical cord tissue removing of part to be exposed, the PBS buffer solution in slot is discarded, runner 6 holds before holding on the other hand
5, the preceding runner 6 for being connected with fixed pin 16 and umbilical cord tissue is extracted out from runner positioning plate 7, rotate by a certain angle (according to
Umbilical cord exposure is removed depending on situation) after, it reinserts in runner positioning plate 7；The other hand is in the same way by 1 turn of rear runner
It moves and is inserted into runner positioning plate 7 after identical angle, and by fixed pin 16 again insertable into matching hole 13, in 12 slot of buffering liquid groove
It is interior that new PBS buffer solution is added to identical height, continue to remove expose portion umbilical cord tissue.
6) above-mentioned process, is repeated, until all removing finishes umbilical cord one week magnificent Tong Shi glue tissue, remaining navel is moved
Arteries and veins, umbilical vein and remnant tissue remove, that is, complete the stripping process of entire China Tong Shi glue.
Claims (6)
1. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue, it is characterised in that: including buffering liquid groove (12), turn
Dynamic setting buffering liquid groove (12) one end preceding runner (6), be rotatably arranged on the rear runner (1) of buffering liquid groove (12) other end with
And it is plugged in the fixed pin between preceding runner (6) and rear runner (1) and for umbilical cord to be fixed on to buffering liquid groove (12) position
(16)；Buffering liquid groove (12) includes bottom plate (9) and two pieces of side plates (10) being fixed on bottom plate (9) and two end plates (11),
It is offered on two end plates (11) centre bore (2), and the outside on two end plates (11) positioned at centre bore (2) is fixed
Equipped with runner positioning plate (7), position corresponding with centre bore (2) of end plate (11) offers respectively on runner positioning plate (7)
The scallop (8) being coupled for preceding runner (6) or rear runner (1)；Preceding runner (6) and rear runner (1) include the wheel of tooth form
It body (4) and is fixed on wheel body (4) and opposite to each other in the handle (5) of buffering liquid groove (12) side；Fixed pin (16) includes one quiet
Arteries and veins needle (14) and two radicular arteries needles (15) run through on the wheel body (4) of preceding runner (6) and offer for three fixed pins (16) respectively
The location hole (3) passed through, and three location holes (3) are distributed on the wheel body (4) of preceding runner (6) in isosceles triangle, in rear runner (1)
Wheel body (4) on be spaced apart and be equipped with multiple matching holes (13), with according to the trend of umbilical cord medium vessels that three fixed pins (16) are logical
Suitable matching hole (13) is crossed to be plugged on rear runner (1).
2. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue according to claim 1, it is characterised in that:
The shape of scallop (8) and wheel body (4) is mutual corresponding six gears shape.
3. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue according to claim 1, it is characterised in that:
Two pieces of runner positioning plates (7) are fixed on the two end plates (11) of buffering liquid groove (12) with welding manner respectively.
4. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue according to claim 1, it is characterised in that:
Centre bore (2) and scallop (8) are concentric, and the aperture of centre bore (2) is greater than scallop (8).
5. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue according to claim 1, it is characterised in that:
Three location holes (3) on the wheel body (4) of preceding runner (6) are evenly spaced in the periphery of the handle (5) of preceding runner (6).
6. a kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue according to claim 5, it is characterised in that:
The height of two pieces of side plates (10) is less than the height of two end plates (11), so that the space triangular prism that three fixed pins (16) surround
Half height is located under the liquid level for completely filling buffer in buffering liquid groove (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339466.7A CN109998601B (en) | 2019-04-25 | 2019-04-25 | A supplementary stripping off device that is used for human umbilical cord periphery huatong glue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910339466.7A CN109998601B (en) | 2019-04-25 | 2019-04-25 | A supplementary stripping off device that is used for human umbilical cord periphery huatong glue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109998601A true CN109998601A (en) | 2019-07-12 |
| CN109998601B CN109998601B (en) | 2021-07-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910339466.7A Expired - Fee Related CN109998601B (en) | 2019-04-25 | 2019-04-25 | A supplementary stripping off device that is used for human umbilical cord periphery huatong glue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109998601B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110136226A1 (en) * | 2009-12-07 | 2011-06-09 | Synthecon, Inc. | Stem cell bioprocessing and cell expansion |
| US20120115224A1 (en) * | 2009-04-13 | 2012-05-10 | Louis Yuge | Culture methods of bone marrow stromal cells and mesenchymal stem cells, and manufacture method of graft cells for central nerve system diseases therapy |
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| CN204474668U (en) * | 2015-02-13 | 2015-07-15 | 首都医科大学 | The Human umbilical vein endothelial cells tripping device improved |
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| CN108486057A (en) * | 2018-04-08 | 2018-09-04 | 天晴干细胞股份有限公司 | The method for extracting candidate stem cell in placenta using multi-functional placenta tissue processing unit |
| CN208081580U (en) * | 2017-12-17 | 2018-11-13 | 遵义医学院附属医院 | A kind of tool obtaining placental blood |
| CN208403671U (en) * | 2017-08-11 | 2019-01-22 | 胡维娜 | A kind of desktop type hand-rail type archives clamping apparatus for placing |
-
2019
- 2019-04-25 CN CN201910339466.7A patent/CN109998601B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120115224A1 (en) * | 2009-04-13 | 2012-05-10 | Louis Yuge | Culture methods of bone marrow stromal cells and mesenchymal stem cells, and manufacture method of graft cells for central nerve system diseases therapy |
| US20110136226A1 (en) * | 2009-12-07 | 2011-06-09 | Synthecon, Inc. | Stem cell bioprocessing and cell expansion |
| CN202428417U (en) * | 2011-12-02 | 2012-09-12 | 东莞市龙健电子有限公司 | 360-degree rotary table |
| CN204297832U (en) * | 2014-04-22 | 2015-04-29 | 国家电网公司 | A kind of earth resistance actinomycetes box |
| CN204474668U (en) * | 2015-02-13 | 2015-07-15 | 首都医科大学 | The Human umbilical vein endothelial cells tripping device improved |
| CN206680468U (en) * | 2017-04-18 | 2017-11-28 | 广州航华生物医药科技有限公司 | A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell |
| CN208403671U (en) * | 2017-08-11 | 2019-01-22 | 胡维娜 | A kind of desktop type hand-rail type archives clamping apparatus for placing |
| CN208081580U (en) * | 2017-12-17 | 2018-11-13 | 遵义医学院附属医院 | A kind of tool obtaining placental blood |
| CN108486057A (en) * | 2018-04-08 | 2018-09-04 | 天晴干细胞股份有限公司 | The method for extracting candidate stem cell in placenta using multi-functional placenta tissue processing unit |
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| Publication number | Publication date |
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| CN109998601B (en) | 2021-07-20 |
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Effective date of registration: 20221213 Address after: 276700 west head of Shuhe street, Linshu County, Linyi City, Shandong Province Patentee after: Linshu Jicai Human Resources Service Co.,Ltd. Address before: 471000 B15, Floor 4, Building 1 #, Yinkun Science Park, No. 6, Fenghua Road, High tech Zone, Luoyang District, China (Henan) Pilot Free Trade Zone, Luoyang City, Henan Province Patentee before: HENAN ZHUOFENG BIOLOGICAL TECHNOLOGY Co.,Ltd. |
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Granted publication date: 20210720 |