CN109997856A - A kind of composition of small molecule compound and its application - Google Patents
A kind of composition of small molecule compound and its application Download PDFInfo
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- CN109997856A CN109997856A CN201910300796.5A CN201910300796A CN109997856A CN 109997856 A CN109997856 A CN 109997856A CN 201910300796 A CN201910300796 A CN 201910300796A CN 109997856 A CN109997856 A CN 109997856A
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- small molecule
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/24—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms
- A01N43/26—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of composition of small molecule compound and its application, the composition includes lipoic acid, Carmofur, Isodon xerophilus element G, xylose and Excisanin B, and the mass ratio of the composition is 8:1:1:6:1.Small molecule compound of the present invention or combinations thereof object has obviously inhibiting effect to the expression of the hrp T3SS of Xcc 8004, it can be almost suppressed to the degree that do not express, while having preferable effect to Biocontrol Effect and the growth of pathogen will not be inhibited in host plant.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of small molecule compound composition and its application.
Background technique
Plant disease is always one of crop production reduction, the Main Factors of quality reduction.At present to the improvement of crop disease
Mainly pass through breeding, cultivation resistant variety and uses chemical pesticide.But breeding, cultivation resistant variety are mainly by anti-source deficiency
Territory restriction or due to pathogen tachytelic evolution variation make resistant variety resistance lose.The extensive use of chemical pesticide causes
Severe environmental pollution, ecology are destroyed, and harmful organism drug resistance is caused to increase and person poultry poisoning's injures and deaths.
Previous nuisanceless antibacterials are usually some antibiotics, and being used for a long time for antibiotic can make bacterium gradually
It develops drug resistance.It would therefore be highly desirable to research and develop new prevent and cure diseases strategy and novel medicine for public health.Novel antibacterials with
T3SS is disease-resistant important target, to achieve the purpose that T3SS is inhibited to prevent disease and not kill pathogen, is avoided that bacterium
It develops drug resistance.
Crucifer black rot bacterium, also referred to as Xanthomonas campestris pv campestris pvs oryzae and oryzicola (Xanthomonas
Campestris pv.campestris, abbreviation Xcc), it is that a kind of all crucifers can be caused in the world black
The gramnegative bacterium (Qian, 2005) of maize ear rot.The bacterium can be planted in any growth period of host by water hole or wound intrusion
In object, cause disease, host includes wild cabbage, cauliflower, Chinese cabbage, radish, rape etc. (Rudolph, 1993).It is typical black
Maize ear rot symptom is the formation V-shaped scab on leaf margin, with the expansion of scab, vein blackening, thus referred to as black rot.Black corruption
Disease is considered as endangering disease the most serious to crucifer, especially in subtropical and tropical zones, temperature and humidity
All it is suitable for the growth and breeding of the bacterium, therefore falls ill more serious (Williams, 1980).Allergic reaction and pathogenic gene
(hypersensitive reaction andpathogenicity, hrp) encoded type III excretory system (type III
Secretion system, T3SS) and its discharged type III effector (type III secretion effectors,
T3SE) be crucifer black rot bacterium the key pathogenetic factor.The bacterium by type III excretory system discharge poisonous effect object because
With host plant Molecular interaction occurs for son, so as to cause plant pathogenic or allergic reaction.Crucifer black rot bacterium hrp gene
Expression by plant signal induction and key regulatory albumen HrpX control.However inhibit crucifer black rot bacterium key pathogenetic
The substance of system hrp T3SS does not have been reported that.
Summary of the invention
The purpose of the present invention is to provide a kind of to cause a disease to crucifer black rot bacterium relevant small molecule compound or group
Object is closed, for inhibiting crucifer black rot bacterium hrp type III excretory system.
The present invention is realized especially by following technical scheme:
A kind of small molecule compound is inhibiting the application in black rot hrp type III excretory system, the small molecule
Compound includes: lipoic acid or Carmofur, and structural formula difference is as follows:
A kind of small molecule compound composition, the composition include lipoic acid, Carmofur, Isodon xerophilus element G, wood
Sugar and Excisanin B.
Preferably, the mass ratio of the lipoic acid, Carmofur, Isodon xerophilus element G, xylose and Excisanin B is 8:
1:1:6:1。
Five kinds of small molecule compounds provided by the present invention are all days screened from national small molecule compound library and plant
Right plant extracts, it is environmentally protective, it can be used as the research object of biological and ecological methods to prevent plant disease, pests, and erosion drug of new generation in the future.
Small molecule compound composition described above is inhibiting the application in black rot hrp type III excretory system
Within protection scope of the present invention.
One of described lipoic acid or Carmofur kind and it is a variety of as preparation prevent and treat crucifer black rot bacterium pesticide
Application in preparation.
Small molecule compound of the invention or combinations thereof object can used before through dilution or directly by user.?
Preparation pesticide-acceptable can be prepared as by common processing method known in those skilled in the art, i.e., by active material
After mixing with liquid solvent or solid carrier, surfactant such as dispersing agent, stabilizer, wetting agent, binder, defoaming are added
One or more of agent, disintegrating agent, antifreeze etc..
The weight percentage of active component is the 0.1%~90% of total weight, preferably 5% in usual pesticidal preparations
~70%.According to different preparation types, active component content range is different.In general, liquid preparation contains by weight
1%~70% active material, preferably 5%~50%；Solid pharmaceutical preparation contains by weight 5%~70% active matter
Matter, preferably 10%~70%.
Small molecule compound of the invention or combinations thereof object can be processed into any pesticide-acceptable dose as needed
Type, wherein more preferably dosage form is wettable powder, water dispersible granules, suspoemulsion, aqueous emulsion, microemulsion or microcapsule suspending agent.
The invention has the benefit that
Small molecule compound of the present invention or combinations thereof object has obviously the expression of the hrp T3SS of Xcc 8004
Inhibiting effect can almost be suppressed to the degree that do not express, while have preferable effect to Biocontrol Effect and will not plant in host
Inhibit the growth of pathogen in object.
Detailed description of the invention
Fig. 1 is that this five kinds of compounds are verified by fluorescing system to the inhibiting effect of the type III excretory system of Xcc 8004；
Fig. 2 is the influence for being added to five kinds of individual compounds to 8004 pathogenicity of Xcc.
Fig. 3 is the detection to the scab length of five kinds of compounds；
Fig. 4 is the detection of growth curve in leaf.
Specific embodiment
Below in conjunction with specific embodiment of the present invention, technical solution of the present invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Used material includes crucifer black rot bacterium Xcc8004 bacterial strain, Xcc in an embodiment of the present invention
8004/0241lux bacterial strain is that this research department saves；Various compounds are both from national small molecule compound library and plant
In, related plant experimental plant has carrot (Raphanus sativus var.radicula all over the body
Cv.Manshenhong) Chengdu seed parent company.
Detection of 1 compound of embodiment to type III fluorescence activity
After strains tested Xcc 8004/0241lux is incubated overnight, OD is measured600Bacterium solution is collected, is then cultivated with XZM
Base washs and thallus is resuspended first by OD600It is adjusted to 1.By starting thallus OD600=0.15 concentration is forwarded in XZM culture medium, so
Bacterium solution packing is added in 96 orifice plates afterwards, every hole adds 100 μ L bacterium solutions.It is control with DMSO, each small molecule presses a certain amount of addition
Into 96 orifice plates, (Isodon xerophilus element G, Excisanin B, Carmofur are 5 μM, and lipoic acid and xylose final concentration are 80 μ
M).3 repetitions of each sample cover 96 orifice plates after having added, and with sealing compound that the rim seal of plate is good, are put into level
In shaking table, 28 DEG C of constant temperature, 600rpm cultivates 20h.Measure the cell density OD of 96 orifice plates600, then bacterium solution be transferred to 96 holes biology
In the detection plate that shines, 0.5% Decanal of 0.5 μ L is added in every hole, uses Synergy H1 global function microplate reader (Biotek)
Luminous intensity is detected, and calculates the relative value of each hole Lux enzyme activity, statistical analysis is carried out to data.
Fluorescence detection shows five kinds of compounds can have highly significant inhibition to make to type III effector gene XC_0241
With (see Fig. 1).
The pathogenic detection of embodiment 2
Testing host plant used is that carrot seedling (plants entitled Raphanus sativus var.radicula all over the body
Cv.Manshenhong), inoculation method used is leaf-cutting method.8004 wild-type strain of Xcc at 28 DEG C with NYGB culture medium into
Row Liquid Culture 15-18h is diluted to OD with the NYGB for being added to a certain concentration compound600=0.001, with sterilized scissors
It after bacterium solution impregnates 5s, is cut at axis in healthy leaves away from the direction of the vertical vein of blade tip 1-2cm, stops 5s, be vaccinated
Plant observes result after 25-30 DEG C of culture 10d.
Pharmacosensitive test show the Xcc 8004 for being added to this five kinds of compounds compared with not adding, pathogenic significant drop
Low (see Fig. 2 and Fig. 3).
The measurement of growth curve in 3 leaf of embodiment
This experiment is detected using coated plate number bacterium colony method, and selection is grown fine, and leaf blade size is almost the same to expire
Body carrot carries out leaf-cutting inoculation.Each sample is at least inoculated with 50 leaves or more, to guarantee the use of later experiments.It has been inoculated with
Later, it was once sampled respectively at the 1st, 3,5,7,9 day, wins 9 leaves at random, every 3 leaves are as one group, at least 3
Secondary repetition.
Sampling is all that each sample takes 9 much the same leaves of size at random every time, is then divided into 3 groups and marks,
Every group of 3 leaves.It adopts leaf back to carry out disinfection to blade using medical disinfecting alcohol in super-clean bench, mainly wipe out
The dust material (being careful when wiping, as far as possible gently, avoid the artificially damage caused by blade) of blade surface.Blade is dried,
Every group of blade is put into sterilized mortar respectively again and it is adequately ground, until homogenate is presented in lapping liquid
Shape.Then the NYGB culture medium of 5mL or the ddH of sterilizing is added2O draws the lapping liquid of 1mL to the sterile centrifugation tube of 1.5mL
In, and carry out 10 × gradient dilution (each extension rate is determined according to the number of days of sampling).The lapping liquid diluted is applied
For cloth into the plate containing corresponding antibiotic, every group of blade of each sample does the repetition of 3 diluted concentrations, and each concentration applies 3
Block plate, every block of plate take the lapping liquid of 100 μ L, mark respectively.Plate is placed into 28 DEG C of incubators after coated plate and is cultivated
3d counts the clump count on plate, by formula X=A × C/5B (A: the average colony number (a) of plate；B: coated plate bacterium solution
Volume (ml)；C: diluted multiple) the clump count X in every leaf is counted.
The log value of daily 3 mean concentrations of each sample obtained by formula is as ordinate, the time of sampling
(number of days) is used as abscissa.Draw the growth curve that bacterium grows in leaf (see Fig. 4).
Growth test show this five kinds of small molecule compounds in host plant leaf for Xcc8004 dynamic growth feelings
Condition there is not difference substantially without influence substantially compared with the control group.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understand without departing from the principles and spirit of the present invention can to these examples carry out it is a variety of variation, modification, replacement and
Modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of composition of small molecule compound, which is characterized in that the composition includes lipoic acid, Carmofur, drought life
Plectranthin G, xylose and Excisanin B, the mass ratio of the composition are 8:1:1:6:1.
2. a kind of small molecule compound is inhibiting the application in black rot hrp type III excretory system, which is characterized in that described
Small molecule compound are as follows: lipoic acid or Carmofur.
3. the composition of small molecule compound described in claim 1 is inhibiting answering in black rot hrpIII type excretory system
With.
4. lipoic acid as claimed in claim 2 or Carmofur are in as preparation prevention and treatment crucifer black rot bacterium pesticidal preparations
Using.
5. application according to claim 4, which is characterized in that the pesticidal preparations are selected from wettable powder, water dispersion
Granula, suspoemulsion, aqueous emulsion, microemulsion or microcapsule suspending agent.
6. composition described in claim 1 is as the application in preparation prevention and treatment crucifer black rot bacterium pesticidal preparations.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111139250A (en) * | 2020-01-03 | 2020-05-12 | 广东食品药品职业学院 | Lipoic acid synthesis related gene and application thereof |
| CN112640907A (en) * | 2021-01-19 | 2021-04-13 | 西南大学 | Novel application of lipoic acid in inhibiting plant fungal pathogenic bacteria and method for preventing and treating citrus fruit postharvest fungal diseases |
| CN115531389A (en) * | 2022-09-21 | 2022-12-30 | 桂林医学院第二附属医院 | Application of carmofur in preparation of medicine for regulating intestinal flora and improving intestinal barrier |
| CN115708828A (en) * | 2022-09-21 | 2023-02-24 | 桂林医学院第二附属医院 | Application of carmofur in preparation of anti-intestinal bacteria medicine |
| CN115886021A (en) * | 2022-05-27 | 2023-04-04 | 广西大学 | Micromolecular compound and composition for inhibiting banana fusarium wilt and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111139250A (en) * | 2020-01-03 | 2020-05-12 | 广东食品药品职业学院 | Lipoic acid synthesis related gene and application thereof |
| CN111139250B (en) * | 2020-01-03 | 2023-04-07 | 广东食品药品职业学院 | Lipoic acid synthesis related gene and application thereof |
| CN112640907A (en) * | 2021-01-19 | 2021-04-13 | 西南大学 | Novel application of lipoic acid in inhibiting plant fungal pathogenic bacteria and method for preventing and treating citrus fruit postharvest fungal diseases |
| CN112640907B (en) * | 2021-01-19 | 2021-11-16 | 西南大学 | Novel application of lipoic acid in inhibiting plant fungal pathogenic bacteria and method for preventing and treating citrus fruit postharvest fungal diseases |
| CN115886021A (en) * | 2022-05-27 | 2023-04-04 | 广西大学 | Micromolecular compound and composition for inhibiting banana fusarium wilt and application thereof |
| CN115531389A (en) * | 2022-09-21 | 2022-12-30 | 桂林医学院第二附属医院 | Application of carmofur in preparation of medicine for regulating intestinal flora and improving intestinal barrier |
| CN115708828A (en) * | 2022-09-21 | 2023-02-24 | 桂林医学院第二附属医院 | Application of carmofur in preparation of anti-intestinal bacteria medicine |
| CN115531389B (en) * | 2022-09-21 | 2024-07-12 | 桂林医学院第二附属医院 | Application of carmofur in preparing medicine for regulating intestinal flora and improving intestinal barrier |
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