CN109988710A - A kind of kit of detection bacterium macrolides drug resistant gene - Google Patents
A kind of kit of detection bacterium macrolides drug resistant gene Download PDFInfo
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- CN109988710A CN109988710A CN201910383705.9A CN201910383705A CN109988710A CN 109988710 A CN109988710 A CN 109988710A CN 201910383705 A CN201910383705 A CN 201910383705A CN 109988710 A CN109988710 A CN 109988710A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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Abstract
The invention discloses a kind of kits of detection bacterium macrolides drug resistant gene, its structure includes detection kit, protect bottom protective cover, display screen, interior dynamic box cover, handle, electric controller, detection kit is by box body, reagent adaptor, vibrating seat, temperature controller, drug resistant gene detector composition, it can more quickly be obtained using time of the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in the rolling movable plate cooperation drug resistant gene detector shaking reagent bottle in interior dynamic box cover in confrontation, it can be with stable equilibrium and regular movement when shaking by reagent bottle by reagent adaptor, and it will not be allowed to be damaged, recycle vibrating seat that the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in reagent bottle is joined up and down, allow it that can obtain the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in different environment There is any difference in confrontation, which can more accurately obtain the drug resistant gene data of detection.
Description
Technical field
The present invention relates to field of biotechnology, particularly with regard to a kind of examination of detection bacterium macrolides drug resistant gene
Agent box.
Background technique
After doctor trained in Western medicine introduction China, due to the irrational use of a large amount of antibiotic clinically, clinical disease Related Bacteria
Drug resistance be continuously increased, cause normal flora imbalance and a large amount of antibody-resistant bacterium occur.Drug-fast bacteria is able to bear antibiolics
The attack of object, so the treatment of standard just loses effect, infects and continues to exist and can infect other people.Antibiotics resistance
Property be caused by improper use by using antibiotic medicine, particular against raw plain drug, when bacterium mutates or obtains
When drug resistant gene, drug resistance is just produced.Extensive use of the Macrocyclolactone lactone kind medicine in clinic is so that more and more generate
To the bacterial strain of such antibiotics resistance, the clinical treatment is caused to fail.This just need it is a kind of can conveniently detection bacterium it is resistance to
The method of pharmacological property, thus to find drug-resistant bacteria early, and then change therapeutic regimen, guiding clinical treatment, but macrolide
Class drug be to be stored in reagent bottle to be constantly in stationary state in the confrontation of the bacterial strain of antibiotics resistance so that the number of detection
According to not accurate enough, it is therefore desirable to it is a kind of can by Macrocyclolactone lactone kind medicine in the confrontation of the bacterial strain of antibiotics resistance in a variety of
Increase the accuracy of detection data in environment.
Summary of the invention
In view of the deficiencies of the prior art, the present invention is to realize by the following technical solutions: a kind of big ring of detection bacterium
The kit of lactone drug resistant gene, structure include detection kit, protect bottom protective cover, display screen, interior dynamic box cover, handle, electricity
Device is controlled, guarantor's bottom protective cover is installed on the detection kit bottom end, the detection kit front end is equipped with display screen, institute
The detection kit stated is connected with display screen, and the detection kit top is equipped with interior dynamic box cover, the detection examination
Agent box and interior dynamic box cover are flexibly connected, and are equipped with handle, the detection kit on the detection kit left and right ends
It is gap-matched with handle, the detection kit right end has electric controller, the detection kit and electric controller
It matches；
The detection kit is made of box body, reagent adaptor, vibrating seat, temperature controller, drug resistant gene detector, described
Tray interior bottom end is equipped with vibrating seat, and the inboard wall of cartridge is equipped with reagent adaptor, and the box body and reagent are accepted
Device is flexibly connected, and the box body front inner wall is equipped with temperature controller, the box body and temperature controller electrical connection, the reagent
Drug resistant gene detector is equipped with above adaptor, the box body and drug resistant gene detector are gap-matched.
As advanced optimizing for the technical program, the reagent adaptor is by fixed frame, reagent bottle slot, sliding
Ring, fixed ring, close ring composition, the fixed frame surface is equipped with nine reagent bottle slots, and pacifies on six bottle slots
Equipped with slip ring, it is additionally provided with close ring under the reagent bottle slot bottom end, the reagent bottle slot and close ring are integrated
Change structure, and fixed ring is installed in three reagent bottle slots.
As advanced optimizing for the technical program, the slip ring further includes sliding pearl, on the slip ring outside
Equipped with sliding pearl, the slip ring and sliding pearl are slidably matched.
As advanced optimizing for the technical program, the close ring further includes closely sealed ring item, in the close ring
Side is equipped with closely sealed ring item, the close ring and closely sealed ring glue connection.
As advanced optimizing for the technical program, the vibrating seat is made of pedestal, semicircle groove, semicircle convex ball,
Three semicircle convex balls are equidistantly distributed in the pedestal inner bottom, the pedestal is fixedly connected with semicircle convex ball, institute
Three semicircle grooves are equidistantly distributed in the pedestal inner bottom stated.
As advanced optimizing for the technical program, the interior dynamic box cover be additionally provided with servo motor, shake movable plate, clip slot,
Rocking bar, ring gear, driving gear, the servo motor bottom end are equipped with driving gear, the servo motor and sliding tooth
Wheel is mechanically connected by motor shaft, and the shaking intralamellar part middle position is equipped with ring gear, the ring gear and driving
Gear is meshed, and six clip slots are equidistantly distributed in the shaking plate surface, are installed on four ends of rolling movable plate angle
There is rocking bar.
As advanced optimizing for the technical program, the fixed frame and drug resistant gene detector, shake movable plate three it
Between be parallel to each other.
As advanced optimizing for the technical program, the closely sealed ring item is made of rubber material, and flexibility is good.
As advanced optimizing for the technical program, the drug resistant gene detector is connected with rolling movable plate by clip slot
It connects.
As advanced optimizing for the technical program, the rolling movable plate is connected with interior dynamic box cover by rocking bar.
Beneficial effect
A kind of kit of detection bacterium macrolides drug resistant gene of the present invention leads to after reagent bottle is inserted into reagent adaptor
Electric controller control servo motor work is crossed, servo motor drives driving gear to rotate by motor shaft, and driving gear cooperates internal tooth
Circle, which drives, to shake movable plate and shakes under dynamic box cover inside, and shaking movable plate can be balanced by it using rocking bar and shake work in the case where stablizing
Make, shakes movable plate using being detected and being shaken in drug resistant gene detector insertion reagent bottle, so that in the big ring in reagent bottle
Esters medicine in the confrontation of the bacterial strain of antibiotics resistance it can be concluded that more data, pass through drug resistant gene detection when shaking movable plate
Device drives reagent bottle when shaking on slip ring, and slip ring shakes reagent bottle using sliding pearl, allows macrolides in reagent bottle
Drug mixes blending with the bacterial strain of antibiotics resistance together, and is matched using the intracorporal semicircle groove of seat and semicircle convex ball
It closes, the bacterial strain of the Macrocyclolactone lactone kind medicine in reagent bottle and antibiotics resistance is joined up and down, allows it can be in difference
Environment in show what difference the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance has in confrontation.
Compared with prior art, the beneficial effects of the present invention are: cooperating drug resistant gene using the rolling movable plate in interior dynamic box cover
Time of the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in detector shaking reagent bottle in confrontation can more accelerate
Fast obtains, can be with stable equilibrium and regular movement when shaking by reagent bottle by reagent adaptor, and will not allow
It is damaged, and vibrating seat is recycled to allow the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in reagent bottle upper and lower
It joins, allows it that can show what the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance has in confrontation in different environment
Difference, the design can more accurately obtain the drug resistant gene data of detection.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1 is a kind of structural schematic diagram of the kit of detection bacterium macrolides drug resistant gene of the present invention.
Fig. 2 is a kind of the schematic diagram of the section structure of the kit of detection bacterium macrolides drug resistant gene of the present invention.
Fig. 3 is the structural schematic diagram of reagent adaptor of the present invention.
Fig. 4 is the enlarged drawing of A in Fig. 2 of the present invention.
Fig. 5 is the structural schematic diagram of vibrating seat of the present invention.
Fig. 6 is the structural schematic diagram that box cover is moved in the present invention.
In figure: detection kit -1 protects bottom protective cover -2, display screen -3, interior dynamic box cover -4, handle -5, electric controller -6, box
Body -1a, reagent adaptor -1b, vibrating seat -1c, temperature controller -1d, drug resistant gene detector -1e, fixed frame -1b1, reagent bottle are inserted
Slot -1b2, slip ring -1b3, fixed ring -1b4, close ring -1b5, sliding pearl -1b31, closely sealed ring item -1b51, pedestal -1c1, semicircle
Groove -1c2, it semicircle convex ball -1c3, servo motor -1f1, shakes movable plate -1f2, clip slot -1f3, rocking bar -1f4, ring gear -1f5, drive
Moving gear -1f6.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment and Detailed description of the invention, the preferred embodiment that the present invention is further explained.
Embodiment
Fig. 1-Fig. 6 is please referred to, the present invention provides a kind of kit of detection bacterium macrolides drug resistant gene, structure
Including detection kit 1, protect bottom protective cover 2, display screen 3, interior dynamic box cover 4, handle 5, electric controller 6,1 bottom of detection kit
It is equipped on end and protects bottom protective cover 2,1 front end of detection kit is equipped with display screen 3, the detection kit 1 and aobvious
Display screen 3 is connected, and 1 top of detection kit is equipped with interior dynamic box cover 4, the detection kit 1 and interior dynamic box cover 4
It is flexibly connected, handle 5 is equipped on 1 left and right ends of detection kit, the detection kit 1 and handle 5 use
Clearance fit, 1 right end of detection kit have electric controller 6, and the detection kit 1 and electric controller 6 match,
The detection kit 1 is by box body 1a, reagent adaptor 1b, vibrating seat 1c, temperature controller 1d, drug resistant gene detector 1e group
At the box body 1a inner bottom is equipped with vibrating seat 1c, and the box body 1a inner wall is equipped with reagent adaptor 1b, described
Box body 1a and reagent adaptor 1b be flexibly connected, the box body 1a front inner wall be equipped with temperature controller 1d, the box body
1a and temperature controller 1d electrical connection, is equipped with drug resistant gene detector 1e above the described reagent adaptor 1b, the box body 1a and
Drug resistant gene detector 1e is gap-matched.
The reagent adaptor 1b is by fixed frame 1b1, reagent bottle slot 1b2, slip ring 1b3, fixed ring 1b4, closure
Ring 1b5 composition, the surface fixed frame 1b1 is equipped with nine reagent bottle slot 1b2, and installs on six bottle slot 1b2
There is slip ring 1b3, be additionally provided with close ring 1b5 under the bottom end reagent bottle slot 1b2, the reagent bottle slot 1b2 and closes
Cyclization 1b5 is integrated, and is equipped with fixed ring 1b4 in three reagent bottle slot 1b2,
Above-mentioned fixed ring 1b4 is a kind of fixed accessory, in the case where allowing reagent bottle to remain stationary in kit
Detection data out.
The slip ring 1b3 further includes sliding pearl 1b31, and the outside slip ring 1b3 is equipped with sliding pearl 1b31, described
Slip ring 1b3 and sliding pearl 1b31 be slidably matched,
Above-mentioned cunning pearl 1b31 be for cooperate slip ring 1b3 sliding when can be more regular, and protect it from can exist
Acutely shake by when to damage.
The close ring 1b5 further includes closely sealed ring 1b51, and the inside close ring 1b5 is equipped with closely sealed ring item
1b51, the close ring 1b5 and closely sealed ring 1b51 glue connection,
Above-mentioned closely sealed ring 1b51 is a kind of for protecting reagent bottle not to be damaged at work.
The vibrating seat 1c is made of pedestal 1c1, semicircle groove 1c2, semicircle convex ball 1c3, inside the pedestal 1c1
Three semicircle convex ball 1c3 are equidistantly distributed on bottom end, the pedestal 1c1 is fixedly connected with semicircle convex ball 1c3, the seat
Three semicircle groove 1c2 are equidistantly distributed in body 1c1 inner bottom.
The interior dynamic box cover 4 be additionally provided with servo motor 1f1, shake movable plate 1f2, clip slot 1f3, rocking bar 1f4, ring gear 1f5,
Gear 1f6 is driven, the bottom end servo motor 1f1 is equipped with driving gear 1f6, the servo motor 1f1 and sliding tooth
It takes turns 1f6 to be mechanically connected by motor shaft, the rolling movable plate 1f2 bosom position is equipped with ring gear 1f5, the internal tooth
Circle 1f5 and driving gear 1f6 are meshed, and six clip slot 1f3 are equidistantly distributed on the surface rolling movable plate 1f2, described
It shakes and rocking bar 1f4 is installed on the angle of tetra- ends movable plate 1f2,
Above-mentioned rocking bar 1f4 is a kind of shaking that rolling movable plate 1f2 can be cooperated to move the lower balance and stability of box cover 4 inside.
The fixed frame 1b1 and drug resistant gene detector 1e, it shakes and is parallel to each other between movable plate 1f2 three, described is close
Cyclization 1b51 is made of rubber material, and flexibility is good, and the drug resistant gene detector 1e and rolling movable plate 1f2 pass through clip slot
1f3 is connected, and the rolling movable plate 1f2 is connected with interior dynamic box cover 4 by rocking bar 1f4.
The principle of the present invention: after reagent bottle is inserted into reagent adaptor 1b, servo motor 1f1 work is controlled by electric controller 6
Make, servo motor 1f1 drives driving gear 1f6 to rotate by motor shaft, and driving gear 1f6 cooperation ring gear 1f5, which drives, to be shaken
Dynamic box cover 4 is lower inside shakes by plate 1f2, shakes movable plate 1f2 and using rocking bar 1f4 it can be balanced and shake work in the case where stablizing
Make, shakes movable plate 1f2 using being detected and being shaken in drug resistant gene detector 1e insertion reagent bottle, so that big in reagent bottle
Cyclic lactone class drug in the confrontation of the bacterial strain of antibiotics resistance it can be concluded that more data, pass through drug resistance when shaking movable plate 1f2
Genetic test device 1e drives reagent bottle when shaking on slip ring 1b3, and slip ring 1b3 shakes reagent bottle using sliding pearl 1b31, allows
Macrocyclolactone lactone kind medicine in reagent bottle mixes blending with the bacterial strain of antibiotics resistance together, and using in pedestal 1c1
Semicircle groove 1c2 is matched with semicircle convex ball 1c3, so that the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in reagent bottle
It can join up and down, allow it that can show that the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance is being fought in different environment
In have what difference.
Solution to the problem of the present invention is: cooperating drug resistant gene detector 1e using the rolling movable plate 1f2 in interior dynamic box cover 4
Shaking time of the bacterial strain of the Macrocyclolactone lactone kind medicine and antibiotics resistance in reagent bottle in confrontation more quick can obtain
Out, by reagent adaptor 1b allow reagent bottle shake when can with stable equilibrium and regular movement, and will not allow its by
To damage, recycle vibrating seat 1c that the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance in reagent bottle is handed over up and down
It closes, allows it that can show what area the bacterial strain of Macrocyclolactone lactone kind medicine and antibiotics resistance has in confrontation in different environment
Not, which can more accurately obtain the drug resistant gene data of detection.
The basic principles, main features and advantages of the present invention have been shown and described above, the technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention can not only be realized in other specific forms under the premise of not departing from spirit of that invention or essential characteristic
The present invention will also have various changes and improvements, these changes and improvements both fall within scope of the claimed invention, therefore this hair
Bright claimed range is defined by appended claims and its equivalent, rather than above description limits.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of kit of detection bacterium macrolides drug resistant gene, structure includes detection kit (1), protects bottom protective cover
(2), display screen (3), interior dynamic box cover (4), handle (5), electric controller (6) are equipped with guarantor on the detection kit (1) bottom end
Bottom protective cover (2), the detection kit (1) front end are equipped with display screen (3), the detection kit (1) and display screen
(3) be connected, the detection kit (1) top be equipped with interior dynamic box cover (4), the detection kit (1) and it is interior move
Box cover (4) is flexibly connected, and is equipped with handle (5), the detection kit on the detection kit (1) left and right ends
(1) it is gap-matched with handle (5), the detection kit (1) right end has electric controller (6), the detection examination
Agent box (1) and electric controller (6) match, it is characterised in that:
The detection kit (1) is by box body (1a), reagent adaptor (1b), vibrating seat (1c), temperature controller (1d), drug resistance base
Because detector (1e) is formed, the box body (1a) inner bottom is equipped with vibrating seat (1c), on the box body (1a) inner wall
Equipped with reagent adaptor (1b), the box body (1a) and reagent adaptor (1b) are flexibly connected, the front end the box body (1a)
Inner wall is equipped with temperature controller (1d), the box body (1a) and temperature controller (1d) electrical connection, on the reagent adaptor (1b)
Side is equipped with drug resistant gene detector (1e), and the box body (1a) and drug resistant gene detector (1e) are gap-matched.
2. a kind of kit of detection bacterium macrolides drug resistant gene according to claim 1, it is characterised in that: institute
The reagent adaptor (1b) stated is by fixed frame (1b1), reagent bottle slot (1b2), slip ring (1b3), fixed ring (1b4), closure
Ring (1b5) composition, the fixed frame surface (1b1) are equipped with nine reagent bottle slots (1b2), and six bottle slots
It is equipped on (1b2) slip ring (1b3), is additionally provided with close ring (1b5) under described bottom end reagent bottle slot (1b2), it is described
Reagent bottle slot (1b2) and close ring (1b5) are integrated, and are equipped with fixation in three reagent bottle slots (1b2)
Ring (1b4).
3. a kind of kit of detection bacterium macrolides drug resistant gene according to claim 2, it is characterised in that: institute
The slip ring (1b3) stated further includes sliding pearl (1b31), and the slip ring outside (1b3) is equipped with sliding pearl (1b31), described
Slip ring (1b3) and sliding pearl (1b31) are slidably matched.
4. a kind of kit of detection bacterium macrolides drug resistant gene according to claim 2, it is characterised in that: institute
The close ring (1b5) stated further includes closely sealed ring item (1b51), and the close ring inside (1b5) is equipped with closely sealed ring item
(1b51), the close ring (1b5) and closely sealed ring item (1b51) glue connection.
5. a kind of kit of detection bacterium macrolides drug resistant gene according to claim 1, it is characterised in that: institute
The vibrating seat (1c) stated is made of pedestal (1c1), semicircle groove (1c2), semicircle convex ball (1c3), and the pedestal (1c1) is internal
Three semicircle convex balls (1c3) are equidistantly distributed on bottom end, the pedestal (1c1) is fixedly connected with semicircle convex ball (1c3), institute
Three semicircle grooves (1c2) are equidistantly distributed in pedestal (1c1) inner bottom stated.
6. a kind of kit of detection bacterium macrolides drug resistant gene according to claim 1, it is characterised in that: institute
The interior dynamic box cover (4) stated is additionally provided with servo motor (1f1), shakes movable plate (1f2), clip slot (1f3), rocking bar (1f4), ring gear
(1f5), driving gear (1f6), the servo motor bottom end (1f1) are equipped with driving gear (1f6), the servo motor
(1f1) and driving gear (1f6) are mechanically connected by motor shaft, and rolling movable plate (1f2) the bosom position is equipped with interior
Gear ring (1f5), the ring gear (1f5) and driving gear (1f6) are meshed, equidistant on described surface rolling movable plate (1f2)
Six clip slots (1f3) are distributed with, rocking bar (1f4) is installed on four ends of rolling movable plate (1f2) angle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910383705.9A CN109988710B (en) | 2019-05-08 | 2019-05-08 | Kit for detecting macrolide drug-resistant genes of bacteria |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910383705.9A CN109988710B (en) | 2019-05-08 | 2019-05-08 | Kit for detecting macrolide drug-resistant genes of bacteria |
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| CN109988710B CN109988710B (en) | 2022-06-24 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
| CN112725172A (en) * | 2021-02-06 | 2021-04-30 | 浙江省人民医院 | Kit for detecting macrolide drug-resistant genes of bacteria |
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