CN109988230B - Application of nucleoporin Nup98a and Nup98b in regulating and controlling flowering time of plants - Google Patents

Abstract

The invention relates to the technical field of plant molecular biology, in particular to application of nucleoporin Nup98a and Nup98b in regulating and controlling plant flowering time. The invention identifies the function of the nucleoporin Nup98 in regulating and controlling the flowering time and the growth cycle of plants, and provides a method for changing the flowering time of plants by using nucleoporin Nup 98. The simultaneous inactivation of Nup98a and Nup98b can lead to early flowering and shortened growth period of Arabidopsis. Due to the high conservation of the Nup98 gene in plants, the application and the method of the nucleoporin Nup98 in the aspects of regulating and controlling the flowering time and the growth cycle of the plants have general popularization and application values in different plants, and provide a method and a basis for solving the problems of flowering asynchronism, growth period control of various plants, photoperiod sensitivity and introduction in plant cross breeding.

Description

Application of nucleoporin Nup98a and Nup98b in regulating and controlling flowering time of plants

Technical Field

The invention relates to the technical field of plant molecular biology, in particular to application of nucleoporin Nup98a and Nup98b in regulating and controlling plant flowering time.

Background

Nuclear Pore Complex (NPC) is the largest protein Complex on the Nuclear membrane of eukaryotic cells, and it is widely involved in many growth and development processes of organisms by regulating the transport of mRNA and protein between the nucleus and cytoplasm. The research on the function of the nucleoporin in the process of regulating and controlling the growth and development of plants and the development of the nucleoporin with important functions on the growth and development of the plants have important significance on the genetic breeding and the crop planting of the plants. At present, only a few NPC components in plants are preliminarily analyzed in function, and the function research of Nucleoporin 98 (Nup98) is not reported. The present invention finds that the Nup98 protein has very high homology in main crops such as soybean, corn, rice, etc. There are two highly homologous Nup98 genes a and b in arabidopsis thaliana, having the same protein domain as human Nup98 protein, namely the N-terminal FG repeat domain and the C-terminal autoproteolytic domain (APD).

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide application of nucleoporins Nup98a and Nup98b in regulating and controlling flowering time of plants.

In order to achieve the purpose, the technical scheme of the invention is as follows:

according to the invention, through the analysis of the promoters of an arabidopsis Nup98a gene (the sequence is shown as SEQ ID NO.3, and the sequence of a coded protein is shown as SEQ ID NO. 1) and a Nup98b gene (the sequence is shown as SEQ ID NO.4, and the sequence of the coded protein is shown as SEQ ID NO. 2), the promoters of the two genes contain a plurality of identical and specific cis-acting elements. Through protein localization experiments, the Nup98a and Nup98b proteins are not only localized on a nuclear membrane, but also widely distributed in a nuclear cytoplasm. Through inactivation experiments on Nup98a and Nup98b genes, T-DNA insertion single mutants of Nup98a and Nup98b genes are found to have no obvious development phenotype, however, a double mutant (Nup98) of Nup98a Nup98b shows an obvious early flowering phenotype, and the Arabidopsis Nup98a and Nup98b are proved to be functionally redundant and jointly regulate the plant flowering process.

Specifically, the invention provides an application of nucleoporin Nup98 in regulating and controlling the flowering time of plants.

The invention provides application of nucleoporin Nup98 in regulation and control of plant growth cycle.

The invention provides application of nucleoporin Nup98 in plant genetic breeding or transgenic plant construction.

The sequence of the nucleoporin Nup98 is any one of the following sequences:

(1) an amino acid sequence shown as SEQ ID NO.1 or SEQ ID NO. 2;

(2) the amino acid sequence of the protein with the same function is obtained by deleting, replacing or inserting one or more amino acids in the amino acid sequence shown in SEQ ID NO.1 or SEQ ID NO. 2;

(3) an amino acid sequence of a protein which has at least 40 percent of homology with the amino acid sequence shown as SEQ ID NO.1 or SEQ ID NO.2 and has the same function.

According to the invention, bioinformatics analysis shows that the Nup98 protein has very high homology in arabidopsis thaliana and main crops such as soybean, corn and rice, so that the Nup98 protein of the main crops such as soybean, corn and rice has the same functions as the arabidopsis thaliana Nup98 protein according to the conservation of the protein sequence and functions.

Therefore, the proteins having the amino acid sequence of the same functional protein obtained by deletion, substitution or insertion of one or more amino acids of the amino acid sequence shown in SEQ ID No.1 or SEQ ID No.2 in (2) or (3) having at least 40% homology to the amino acid sequence shown in SEQ ID No.1 or SEQ ID No.2 and having the amino acid sequence of the same functional protein include, but are not limited to, nucleopore protein Nup98 of soybean (2 nucleopore proteins Nup98, glyma.12g235000 and glyma.13g26410 are present in soybean), nucleopore protein Nup98 of corn (2 nucleopore proteins Nup98, GRMZM2G 0515 and GRMZM2G 3424 are present in corn), nucleopore protein Nup98 of rice (2 nucleopore proteins Nup98, LOC _ Os12G06890 and grmz _ Os12G 905634), and nucleopore protein Nup 3598 or mutant having the same function as LOC p 3598 or LOC 3598 in rice (2).

Further, the invention provides an application of the coding gene of the nucleoporin Nup98 or the biological material containing the coding gene in regulating and controlling the flowering time of plants, regulating and controlling the growth cycle of the plants, carrying out genetic breeding on the plants or constructing transgenic plants.

Preferably, the nucleotide sequence of the coding gene of Nup98 is any one of the following:

(1) a nucleotide sequence shown as SEQ ID NO.3 or SEQ ID NO. 4;

(2) the nucleotide sequence of the protein with the same function is obtained by deleting, replacing or inserting one or more nucleotides in the nucleotide sequence shown in SEQ ID NO.3 or SEQ ID NO. 4;

(3) nucleotide sequence which can be hybridized with the nucleotide sequence shown as SEQ ID NO.3 or SEQ ID NO.4 under strict conditions.

It will be understood by those skilled in the art that, given the amino acid sequence of the Nup98 protein, different nucleotide sequences encoding genes encoding proteins having the same function as the Nup98a or Nup98b protein may be obtained according to the degeneracy of the codon.

Preferably, the biological material comprises an expression cassette, a vector, a transposon, an engineered bacterium, a host cell or a transgenic cell line.

Experiments prove that nucleoporin Nup98 is a negative control factor for plant flowering, namely, inactivated nucleoporin Nup98 can advance the flowering time of plants or shorten the growth period.

Therefore, the application of the nucleoporin Nup98 of the invention is to lead the flowering time of plants to be advanced or the growth period to be shortened by inactivating the nucleoporin Nup98 or reducing the expression of the nucleoporin Nup 98.

In the invention, the plants comprise corn, soybean, rice, wheat and arabidopsis thaliana.

As an embodiment of the present invention, when the plant is Arabidopsis thaliana, the flowering-time or the growth-period of Arabidopsis thaliana is advanced by inactivating both Nup98a having a sequence shown in SEQ ID NO.1 and Nup98b having a sequence shown in SEQ ID NO. 2.

Furthermore, the invention also provides a method for regulating and controlling the flowering time of the plant, regulating and controlling the growth cycle or constructing the transgenic plant, which is realized by regulating and controlling the expression of nucleoporin Nup98 of the plant.

Preferably, the method for regulating the flowering time of plants, regulating the growth cycle or constructing transgenic plants leads the flowering time of plants to be advanced or the growth period to be shortened by inactivating nucleoporin Nup98 or reducing the expression of nucleoporin Nup 98.

The invention has the beneficial effects that: the invention firstly identifies the function of the nucleoporin Nup98 in regulating and controlling the flowering time and the growth cycle of plants, and provides a method for changing the flowering time of plants by using the mutation of the nucleoporin Nup 98. Experiments prove that Nup98 double-mutant arabidopsis thaliana with Nup98a and Nup98b inactivated simultaneously shows development phenotypes such as early flowering and shortened growth period. Because the Nup98 gene is highly conserved in plants, especially in main crops such as corn, rice, soybean and the like, the application and method of the nucleoporin Nup98 in the aspects of regulating and controlling the flowering time and the growth cycle of the plants have general popularization and application values in different plants, and a method and basis are provided for solving the problem of flowering asynchronism in plant cross breeding, the problem of growth period control of various crops, vegetables, fruits and flowers, the problem of photoperiod sensitivity and the problem of introduction.

Drawings

FIG. 1 is a structural pattern diagram of Nup98 protein and the alignment result of the structural domain sequence in example 1 of the present invention; wherein, A is a structural pattern diagram of arabidopsis Nup98 and human Nup98-96 precursor; b is the alignment of arabidopsis Nup98 and human Nup98 protein autozymolysis structural domain sequences; c is the similarity analysis of the autoproteolytic structure sequences of arabidopsis Nup98 and human Nup 98; d is the sequence similarity analysis between Arabidopsis Nup98 and human Nup98 protein.

FIG. 2 is a schematic structural diagram of a cloning intermediate vector pGWCm according to example 3 of the present invention.

FIG. 3 is a schematic structural diagram of the cloning intermediate vector FU79-GFP of example 4 of the present invention.

FIG. 4 is a schematic structural diagram of an intermediate carrier FU76-35S according to embodiment 4 of the present invention.

FIG. 5 is a schematic structural view of a plant expression vector FU39-2(intron) according to example 4 of the present invention.

FIG. 6 is the subcellular localization of Nup98 protein in example 5 of the present invention, in which GFP represents that Nup98 protein localizes to the nucleus, PI represents cell wall staining, Merge represents the superposition of the first two pictures, and Bright Field represents the Bright Field.

FIG. 7 is the identification of T-DNA insertion mutant of Nup98 gene of Arabidopsis thaliana in example 6 of the present invention, wherein a is the phenotype of T-DNA insertion mutant of Nup98 gene and the scale is 2 cm; b is a structural schematic diagram of the T-DNA insertion of the Nup98 gene, the inverted triangle represents the T-DNA insertion site, the black and gray boxes represent the exon and 5 'or 3' untranslated region, respectively, the black line represents the intron, and the horizontal line below the gene structure represents the RT-PCR product position; c is the transcription level of the Nup98 gene in wild type and mutant analyzed by RT-PCR, and ACTIN2 is an internal reference gene; d is the expression level detection of Nup98a protein in wild type and mutant, and HSP90 is reference protein.

FIG. 8 is a phenotype of flowering of wild type and mutant in example 7 of the present invention, a is a phenotype of flowering of wild type (Col), Nup98a, Nup98b gene single mutant (Nup98a1, Nup98a2, Nup98b1) and double mutant (Nup98a1Nup98b1 and Nup98a2Nup98b1) and transgenic plant (35S: GFP: Nup98b Nup98a1Nup98b1) complemented with Nup98b gene, the scale is 2 cm; b is the statistics of the flowering time of the wild type and mutant plants shown in a, expressed as the number of rosette leaves at the time of flowering (n.gtoreq.30), and error bars indicate standard deviation, significance level (P < 0.01).

Detailed Description

Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example 1Nup98 protein structural Pattern map and Domain sequence alignment

There is a pair of Nup98 proteins with very high homology in the arabidopsis genome: nup98a and Nup98 b. They are encoded by the Nup98a (At1g10390) and Nup98b (At1g59660) genes, respectively. The above gene sequences can be obtained from The Arabidopsis Information Resource database (The Arabidopsis Information Resource, TAIR, http:// www.arabidopsis.org /). The software DNAMAN used for sequence alignment analyzed Nup98a and Nup98b for amino acid sequence similarity of 60.8%. Alignment in The conserved domain database in The international Center for Biotechnology Information (NCBI) database shows that arabidopsis Nup98a and Nup98b proteins have The same protein domains as human Nup98 protein, i.e., N-terminal FG repeat domain and C-terminal autoproteolytic domain (APD) (fig. 1 a). However, in studies of vertebrate nucleoporin function, Nup98 was found to result from HFS cleavage of the Nup98-Nup96 precursor protein via the autoproteolytic site at the C-terminus of the APD domain, with cleavage occurring between F and S; whereas in arabidopsis, Nup98a and Nup98b are both encoded by separate genes. Notably, comparing the APD domains of arabidopsis Nup98a and Nup98b with the APD structure of human Nup98, respectively, the domain similarities were 37.6% and 44.2%, respectively, while the similarity between the APD domains of arabidopsis Nup98a and Nup98b was as high as 78.5%, and in arabidopsis Nup98 protein, HFS (B, C, D of fig. 1) remained at the autoproteolytic site at the C-terminus of the APD domain, indicating that Nup98 may have a common ancestor evolutionarily. The results show that Nup98 domain retains high conservation, although it undergoes different differentiation courses in animals and plants.

EXAMPLE 2 cloning of the Nup98 Gene

Using the forward primer 1: ATGTTTGGCTCATCTAATCCTTT (shown as SEQ ID NO. 5), reverse primer 1: CTAAACTCCATCTTCTTCATCTTC (shown as SEQ ID NO. 6) and forward primer 2: ATGTTCGGTTCTTCTAATAATAAT (shown as SEQ ID NO. 7), reverse primer 2: CTACACATCATATTCATCTCCAAG (shown as SEQ ID NO. 8), obtaining Nup98a and Nup98b from Arabidopsis thaliana by cloning respectively and sequencing, wherein the gene sequences of Nup98a and Nup98b are shown as SEQ ID NO.3 and SEQ ID NO.4 respectively; the amino acid sequences of the protein coded by the protein are respectively shown as SEQ ID NO.1 and SEQ ID NO. 2.

The PCR reaction program of the above-mentioned clone Nup98a and Nup98b genes was pre-denaturation at 95 ℃ for 5min, 30s at 94 ℃, 35s at 55 ℃, 1min at 72 ℃ for 30s, 25 cycles, and extension at 72 ℃ for 10 min.

EXAMPLE 3 construction of cloning vector for Arabidopsis Nup98 Gene

The PCR product amplified in example 2 was directly cloned into pGWGCm (shown in FIG. 2) according to the TA cloning method. Before cloning, pGWGCm was previously hydrolyzed with Ahd I endonuclease to obtain a T vector. The ligation product is transformed into escherichia coli DH5 alpha, and is propagated in the escherichia coli DH5 alpha, and the sequences of Nup98a and Nup98b shown in SEQ ID NO.3 and SEQ ID NO.4 are obtained by sequencing and screening the positive clone.

Example 4 construction of expression vector for Arabidopsis Nup98 Gene

Nup98a gene was digested and ligated to FU79-GFP vector (shown in FIG. 3) using Nup98a cloning vector obtained in example 3 as a template, FU79-GPF-Nup98a, vector FU76-35S (shown in FIG. 4) and plant expression vector FU39-2(intron) (shown in FIG. 5) were mixed at equal ratio and then subjected to LR reaction (reaction system: 50ng each of plasmid, 1. mu.l of LR enzyme, and H supplementation₂O to a final volume of 5. mu.l; reaction conditions are as follows: after mixing evenly, reacting for more than 6 hours at 25 ℃), constructing and obtaining the plant expression vector FU39-2-35S, GFP and Nup98 a. FU39-2-35S GFP Nup98b was constructed as described above. The two expression vectors described above were used to overview the localization and phenotypic analysis of Nup98a and Nup98b in plants.

Example 5 subcellular localization of the Arabidopsis Nup98 Gene

By using the expression vector constructed in example 4, over-expression Arabidopsis plants of 35S, GFP, Nup98a and 35S, GFP, Nup98b were obtained by the Arabidopsis flower dipping method, respectively. The localization conditions of GFP-Nup98a and GFP-Nup98b proteins in transgenic plants are observed by using a laser confocal microscope, and the results are shown in figure 6, wherein Nup98a and Nup98b proteins in Arabidopsis are not only localized on nuclear membranes, but also widely distributed in nuclear cytoplasm, and the localization conditions are consistent with the subcellular distribution characteristics of Nup98 of metazoans reported in the prior art.

Example 6 identification of T-DNA insertion mutants of the Arabidopsis Nup98 Gene

Single mutants of T-DNA insertion were ordered from the Arabidopsis Biological Resource Center (ABRC) nup98a and nup98b, respectively: and they were named Nup98a-1 (SALK-015016), Nup98a-2 (SALK-103803) and Nup98b-1 (GABI-288A 08), respectively, in which T-DNA was inserted in the 3 rd and 2 nd exons of Nup98a gene, respectively, in the Nup98a-1 and Nup98a-2 mutants, and in the Nup98b-1 mutant, in the last intron of Nup98b (b of FIG. 7). After screening for homozygous mutants by PCR, the resulting single mutants of each of Nup98a and Nup98b were found to be devoid of any significant difference in developmental phenotype compared to the wild type by phenotypic analysis (a of figure 7).

The three single mutants were subjected to molecular characterization, and the results of RT-PCR showed that no transcript of the corresponding gene was detected in each single mutant (no transcript of nup98a was detected in both the nup98a-1 and nup98a-2 mutants, and no transcript of nup98b was detected in the nup98b-1 mutant). Moreover, the expression level of the Nup98a gene in the wild type is obviously higher than that of the Nup98b gene, and when the Nup98a gene is mutated, the expression level of the Nup98b gene in vivo is increased; after the Nup98b gene is mutated, the expression level of the Nup98a gene is obviously improved (c of figure 7).

Protein level detection using the Nup98a protein specific monoclonal antibody revealed that no accumulation of Nup98a protein was detected in both Nup98a-1 and Nup98a-2 single mutants, whereas the expression level of Nup98a protein was significantly increased in the Nup98b-1 mutant (fig. 7 d). The above results indicate that the Nup98a gene and the Nup98b gene may be functionally redundant to jointly regulate the growth and development process of Arabidopsis thaliana.

In order to further research the functions of the Nup98a gene and the Nup98b gene, two simultaneous-inactivation Nup98 double mutants of the Nup98a gene and the Nup98b gene, namely Nup98a-1Nup98b-1(Nup98-1) and Nup98a-2Nup98b-1(Nup98-2), are respectively constructed through hybridization.

The method used for the hybridization was as follows: removing the male bud of the female parent of Arabidopsis thaliana to be bloomed, taking down the stamen of the bloomed male parent, and artificially pollinating the pollen of the male parent onto the stigma of the female parent. And (3) harvesting hybrid seeds after the siliques are mature, namely F1 generation, selfing F1 generation to obtain F2 generation, and identifying the genotype of the hybridized plants by using a PCR method in F2 generation.

Example 7 flowering time statistics of 7 nup98 mutants

As a result of analyzing the flowering-time of each of the Nup98 mutants in which Nup98a gene and Nup98b gene were individually and simultaneously inactivated, as shown in FIG. 8, under long-day conditions, the flowering-time of three single mutants, Nup98a-1, Nup98a-2 and Nup98b-1, did not significantly change compared to the number of rosette leaves (11.7. + -. 0.5 pieces) at the time of wild-type flowering, and the number of rosette leaves at the time of flowering was 12. + -. 0.7 pieces, 11.3. + -. 1.1 pieces and 11.3. + -. 0.5 pieces, respectively. However, Nup98 double mutants of which the Nup98a and Nup98b genes are simultaneously inactivated, namely Nup98-1(9.8 +/-0.4 tablets) and Nup98-2(9.1 +/-0.8 tablets), show early flowering phenotype. In the double-mutant background, the early-flowering phenotype of the double mutant can be complemented by using a 35S promoter to regulate the expression of the Nup98b gene, and the number of rosette leaves at the time of flowering is 10.8 +/-1.1.

The results indicate that Nup98 acts as a negative regulator of flowering in arabidopsis, and that the early flowering phenotype of the arabidopsis Nup98 double mutant is indeed caused by loss of Nup98 function (simultaneous inactivation of the Nup98a gene and the Nup98b gene).

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Sequence listing

<110> institute of crop science of Chinese academy of agricultural sciences

Application of <120> nucleoporin Nup98a and Nup98b in regulating and controlling flowering time of plants

<130> KHP191111424.4

<160> 8

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1041

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 1

Met Phe Gly Ser Ser Asn Pro Phe Gly Gln Ser Ser Gly Thr Ser Pro

1 5 10 15

Phe Gly Ser Gln Ser Leu Phe Gly Gln Thr Ser Asn Thr Ser Ser Asn

20 25 30

Asn Pro Phe Ala Pro Ala Thr Pro Phe Gly Thr Ser Ala Pro Phe Ala

35 40 45

Ala Gln Ser Gly Ser Ser Ile Phe Gly Ser Thr Ser Thr Gly Val Phe

50 55 60

Gly Ala Pro Gln Thr Ser Ser Pro Phe Ala Ser Thr Pro Thr Phe Gly

65 70 75 80

Ala Ser Ser Ser Pro Ala Phe Gly Asn Ser Thr Pro Ala Phe Gly Ala

85 90 95

Ser Pro Ala Ser Ser Pro Phe Gly Gly Ser Ser Gly Phe Gly Gln Lys

100 105 110

Pro Leu Gly Phe Ser Thr Pro Gln Ser Asn Pro Phe Gly Asn Ser Thr

115 120 125

Gln Gln Ser Gln Pro Ala Phe Gly Asn Thr Ser Phe Gly Ser Ser Thr

130 135 140

Pro Phe Gly Ala Thr Asn Thr Pro Ala Phe Gly Ala Pro Ser Thr Pro

145 150 155 160

Ser Phe Gly Ala Thr Ser Thr Pro Ser Phe Gly Ala Ser Ser Thr Pro

165 170 175

Ala Phe Gly Ala Thr Asn Thr Pro Ala Phe Gly Ala Ser Asn Ser Pro

180 185 190

Ser Phe Gly Ala Thr Asn Thr Pro Ala Phe Gly Ala Ser Pro Thr Pro

195 200 205

Ala Phe Gly Ser Thr Gly Thr Thr Phe Gly Asn Thr Gly Phe Gly Ser

210 215 220

Gly Gly Ala Phe Gly Ala Ser Asn Thr Pro Ala Phe Gly Ala Ser Gly

225 230 235 240

Thr Pro Ala Phe Gly Ala Ser Gly Thr Pro Ala Phe Gly Ala Ser Ser

245 250 255

Thr Pro Ala Phe Gly Ala Ser Ser Thr Pro Ala Phe Gly Ala Ser Ser

260 265 270

Thr Pro Ala Phe Gly Gly Ser Ser Thr Pro Ser Phe Gly Ala Ser Asn

275 280 285

Thr Ser Ser Phe Ser Phe Gly Ser Ser Pro Ala Phe Gly Gln Ser Thr

290 295 300

Ser Ala Phe Gly Ser Ser Ala Phe Gly Ser Thr Pro Ser Pro Phe Gly

305 310 315 320

Gly Ala Gln Ala Ser Thr Pro Thr Phe Gly Gly Ser Gly Phe Gly Gln

325 330 335

Ser Thr Phe Gly Gly Gln Gln Gly Gly Ser Arg Ala Val Pro Tyr Ala

340 345 350

Pro Thr Val Glu Ala Asp Thr Gly Thr Gly Thr Gln Pro Ala Gly Lys

355 360 365

Leu Glu Ser Ile Ser Ala Met Pro Ala Tyr Lys Glu Lys Asn Tyr Glu

370 375 380

Glu Leu Arg Trp Glu Asp Tyr Gln Arg Gly Asp Lys Gly Gly Pro Leu

385 390 395 400

Pro Ala Gly Gln Ser Pro Gly Asn Ala Gly Phe Gly Ile Ser Pro Ser

405 410 415

Gln Pro Asn Pro Phe Ser Pro Ser Pro Ala Phe Gly Gln Thr Ser Ala

420 425 430

Asn Pro Thr Asn Pro Phe Ser Ser Ser Thr Ser Thr Asn Pro Phe Ala

435 440 445

Pro Gln Thr Pro Thr Ile Ala Ser Ser Ser Phe Gly Thr Ala Thr Ser

450 455 460

Asn Phe Gly Ser Ser Pro Phe Gly Val Thr Ser Ser Ser Asn Leu Phe

465 470 475 480

Gly Ser Gly Ser Ser Thr Thr Thr Ser Val Phe Gly Ser Ser Ser Ala

485 490 495

Phe Gly Thr Thr Thr Pro Ser Pro Leu Phe Gly Ser Ser Ser Thr Pro

500 505 510

Gly Phe Gly Ser Ser Ser Ser Ile Phe Gly Ser Ala Pro Gly Gln Gly

515 520 525

Ala Thr Pro Ala Phe Gly Asn Ser Gln Pro Ser Thr Leu Phe Asn Ser

530 535 540

Thr Pro Ser Thr Gly Gln Thr Gly Ser Ala Phe Gly Gln Thr Gly Ser

545 550 555 560

Ala Phe Gly Gln Phe Gly Gln Ser Ser Ala Pro Ala Phe Gly Gln Asn

565 570 575

Ser Ile Phe Asn Lys Pro Ser Thr Gly Phe Gly Asn Met Phe Ser Ser

580 585 590

Ser Ser Thr Leu Thr Thr Ser Ser Ser Ser Pro Phe Gly Gln Thr Met

595 600 605

Pro Ala Gly Val Thr Pro Phe Gln Ser Ala Gln Pro Gly Gln Ala Ser

610 615 620

Asn Gly Phe Gly Phe Asn Asn Phe Gly Gln Asn Gln Ala Ala Asn Thr

625 630 635 640

Thr Gly Asn Ala Gly Gly Leu Gly Ile Phe Gly Gln Gly Asn Phe Gly

645 650 655

Gln Ser Pro Ala Pro Leu Asn Ser Val Val Leu Gln Pro Val Ala Val

660 665 670

Thr Asn Pro Phe Gly Thr Leu Pro Ala Met Pro Gln Ile Ser Ile Asn

675 680 685

Gln Gly Gly Asn Ser Pro Ser Ile Gln Tyr Gly Ile Ser Ser Met Pro

690 695 700

Val Val Asp Lys Pro Ala Pro Val Arg Ile Ser Ser Leu Leu Thr Ser

705 710 715 720

Arg His Leu Leu His Arg Arg Val Arg Leu Pro Ala Arg Lys Tyr Arg

725 730 735

Pro Gly Glu Asn Gly Pro Lys Val Pro Phe Phe Thr Asp Asp Glu Glu

740 745 750

Ser Ser Ser Thr Pro Lys Ala Asp Ala Leu Phe Ile Pro Arg Glu Asn

755 760 765

Pro Arg Ala Leu Val Ile Arg Pro Val Gln Gln Trp Ser Ser Arg Asp

770 775 780

Lys Ser Ile Leu Pro Lys Glu Gln Arg Pro Thr Ala Pro Leu His Asp

785 790 795 800

Asn Gly Lys Ser Pro Asp Met Ala Thr Asp Ala Ala Asn His Asp Arg

805 810 815

Asn Gly Asn Gly Glu Leu Gly Ala Thr Gly Glu Arg Ile His Thr Ser

820 825 830

Val Asn Ala Asn Gln Lys Pro Asn Gly Thr Thr Arg Ser Asp Gln Ala

835 840 845

Ser Glu Lys Glu Arg Pro Tyr Lys Thr Leu Ser Gly His Arg Ala Gly

850 855 860

Glu Ala Ala Ile Val Tyr Glu His Gly Ala Asp Ile Glu Ala Leu Met

865 870 875 880

Pro Lys Leu Arg Gln Ser Asp Tyr Phe Thr Glu Pro Arg Ile Gln Glu

885 890 895

Leu Ala Ala Lys Glu Arg Ala Asp Pro Gly Tyr Cys Arg Arg Val Arg

900 905 910

Asp Phe Val Val Gly Arg His Gly Tyr Gly Ser Ile Lys Phe Met Gly

915 920 925

Glu Thr Asp Val Arg Arg Leu Asp Leu Glu Ser Leu Val Gln Phe Asn

930 935 940

Thr Arg Glu Val Ile Val Tyr Met Asp Glu Ser Lys Lys Pro Ala Val

945 950 955 960

Gly Gln Gly Leu Asn Lys Pro Ala Glu Val Thr Leu Leu Asn Ile Lys

965 970 975

Cys Ile Asp Lys Lys Thr Gly Lys Gln Phe Thr Glu Gly Glu Arg Val

980 985 990

Glu Lys Tyr Lys Met Met Leu Lys Lys Lys Ala Glu Ala Gln Gly Ala

995 1000 1005

Glu Phe Val Ser Phe Asp Pro Val Lys Gly Glu Trp Lys Phe Arg Val

1010 1015 1020

Glu His Phe Ser Ser Tyr Lys Leu Gly Asp Glu Asp Glu Glu Asp Gly

1025 1030 1035 1040

Val

<210> 2

<211> 997

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 2

Met Phe Gly Ser Ser Asn Asn Asn Pro Phe Gly Gln Ser Ser Ile Ser

1 5 10 15

Ser Pro Phe Gly Thr Gln Thr His Ser Leu Phe Gly Gln Thr Asn Asn

20 25 30

Asn Ala Ser Asn Asn Pro Phe Ala Thr Lys Pro Phe Gly Thr Ser Thr

35 40 45

Pro Phe Gly Ala Gln Thr Gly Ser Ser Met Phe Gly Gly Thr Ser Thr

50 55 60

Gly Val Phe Gly Ala Pro Gln Thr Ser Ser Pro Phe Gly Ala Ser Pro

65 70 75 80

Gln Ala Phe Gly Ser Ser Thr Gln Ala Phe Gly Ala Ser Ser Thr Pro

85 90 95

Ser Phe Gly Ser Ser Asn Ser Pro Phe Gly Gly Thr Ser Thr Phe Gly

100 105 110

Gln Lys Ser Phe Gly Leu Ser Thr Pro Gln Ser Ser Pro Phe Gly Ser

115 120 125

Thr Thr Gln Gln Ser Gln Pro Ala Phe Gly Asn Ser Thr Phe Gly Ser

130 135 140

Ser Thr Pro Phe Gly Ala Ser Thr Thr Pro Ala Phe Gly Ala Ser Ser

145 150 155 160

Thr Pro Ala Phe Gly Val Ser Asn Thr Ser Gly Phe Gly Ala Thr Asn

165 170 175

Thr Pro Gly Phe Gly Ala Thr Asn Thr Thr Gly Phe Gly Gly Ser Ser

180 185 190

Thr Pro Gly Phe Gly Ala Ser Ser Thr Pro Ala Phe Gly Ser Thr Asn

195 200 205

Thr Pro Ala Phe Gly Ala Ser Ser Thr Pro Leu Phe Gly Ser Ser Ser

210 215 220

Ser Pro Ala Phe Gly Ala Ser Pro Ala Pro Ala Phe Gly Ser Ser Gly

225 230 235 240

Asn Ala Phe Gly Asn Asn Thr Phe Ser Ser Gly Gly Ala Phe Gly Ser

245 250 255

Ser Ser Thr Pro Thr Phe Gly Ala Ser Asn Thr Ser Ala Phe Gly Ala

260 265 270

Ser Ser Ser Pro Ser Phe Asn Phe Gly Ser Ser Pro Ala Phe Gly Gln

275 280 285

Ser Thr Ser Ala Phe Gly Ser Ser Ser Phe Gly Ser Thr Gln Ser Ser

290 295 300

Leu Gly Ser Thr Pro Ser Pro Phe Gly Ala Gln Gly Ala Gln Ala Ser

305 310 315 320

Thr Ser Thr Phe Gly Gly Gln Ser Thr Ile Gly Gly Gln Gln Gly Gly

325 330 335

Ser Arg Val Ile Pro Tyr Ala Pro Thr Thr Asp Thr Ala Ser Gly Thr

340 345 350

Glu Ser Lys Ser Glu Arg Leu Gln Ser Ile Ser Ala Met Pro Ala His

355 360 365

Lys Gly Lys Asn Met Glu Glu Leu Arg Trp Glu Asp Tyr Gln Arg Gly

370 375 380

Asp Lys Gly Gly Gln Arg Ser Thr Gly Gln Ser Pro Glu Gly Ala Gly

385 390 395 400

Phe Gly Val Thr Asn Ser Gln Pro Ser Ile Phe Ser Thr Ser Pro Ala

405 410 415

Phe Ser Gln Thr Pro Val Asn Pro Thr Asn Pro Phe Ser Gln Thr Thr

420 425 430

Pro Thr Ser Asn Thr Asn Phe Ser Pro Ser Phe Ser Gln Pro Thr Thr

435 440 445

Pro Ser Phe Gly Gln Pro Thr Thr Pro Ser Phe Arg Ser Thr Val Ser

450 455 460

Asn Thr Thr Ser Val Phe Gly Ser Ser Ser Ser Leu Thr Thr Asn Thr

465 470 475 480

Ser Gln Pro Leu Gly Ser Ser Ile Phe Gly Ser Thr Pro Ala His Gly

485 490 495

Ser Thr Pro Gly Phe Ser Ile Gly Gly Phe Asn Asn Ser Gln Ser Ser

500 505 510

Pro Leu Phe Gly Ser Asn Pro Ser Phe Ala Gln Asn Thr Thr Pro Ala

515 520 525

Phe Ser Gln Thr Ser Pro Leu Phe Gly Gln Asn Thr Thr Pro Ala Leu

530 535 540

Gly Gln Ser Ser Ser Val Phe Gly Gln Asn Thr Asn Pro Ala Leu Val

545 550 555 560

Gln Ser Asn Thr Phe Ser Thr Pro Ser Thr Gly Phe Gly Asn Thr Phe

565 570 575

Ser Ser Ser Ser Ser Leu Thr Thr Ser Ile Ser Pro Phe Gly Gln Ile

580 585 590

Thr Pro Ala Val Thr Pro Phe Gln Ser Ala Gln Pro Thr Gln Pro Leu

595 600 605

Gly Ala Phe Gly Phe Asn Asn Phe Gly Gln Thr Gln Ile Ala Asn Thr

610 615 620

Thr Asp Ile Ala Gly Ala Met Gly Thr Phe Ser Gln Gly Asn Phe Lys

625 630 635 640

Gln Gln Pro Ala Leu Gly Asn Ser Ala Val Met Gln Pro Thr Pro Val

645 650 655

Thr Asn Pro Phe Gly Thr Leu Pro Ala Leu Pro Gln Ile Ser Ile Ala

660 665 670

Gln Gly Gly Asn Ser Pro Ser Ile Gln Tyr Gly Ile Ser Ser Met Pro

675 680 685

Val Val Asp Lys Pro Ala Pro Val Arg Val Ser Pro Leu Leu Thr Ser

690 695 700

Arg His Leu Leu Gln Arg Arg Val Arg Leu Pro Thr Arg Lys Tyr Arg

705 710 715 720

Pro Ser Asp Asp Gly Pro Lys Val Pro Phe Phe Ser Asp Glu Glu Glu

725 730 735

Asn Ser Ser Thr Pro Lys Ala Asp Ala Phe Phe Ile Pro Arg Glu Asn

740 745 750

Pro Arg Ala Leu Phe Ile Arg Pro Val Glu Arg Val Lys Ser Glu His

755 760 765

Pro Lys Asp Ser Pro Thr Pro Leu Gln Glu Asn Gly Lys Arg Ser Asn

770 775 780

Gly Val Thr Asn Gly Ala Asn His Glu Thr Lys Asp Asn Gly Ala Ile

785 790 795 800

Arg Glu Ala Pro Pro Val Lys Val Asn Gln Lys Gln Asn Gly Thr His

805 810 815

Glu Asn His Gly Gly Asp Lys Asn Gly Ser His Ser Ser Pro Ser Gly

820 825 830

Ala Asp Ile Glu Ser Leu Met Pro Lys Leu His His Ser Glu Tyr Phe

835 840 845

Thr Glu Pro Arg Ile Gln Glu Leu Ala Ala Lys Glu Arg Val Glu Gln

850 855 860

Gly Tyr Cys Lys Arg Val Lys Asp Phe Val Val Gly Arg His Gly Tyr

865 870 875 880

Gly Ser Ile Lys Phe Leu Gly Glu Thr Asp Val Cys Arg Leu Asp Leu

885 890 895

Glu Met Val Val Gln Phe Lys Asn Arg Glu Val Asn Val Tyr Met Asp

900 905 910

Glu Ser Lys Lys Pro Pro Val Gly Gln Gly Leu Asn Lys Pro Ala Val

915 920 925

Val Thr Leu Leu Asn Ile Lys Cys Met Asp Lys Lys Thr Gly Thr Gln

930 935 940

Val Met Glu Gly Glu Arg Leu Asp Lys Tyr Lys Glu Met Leu Lys Arg

945 950 955 960

Lys Ala Gly Glu Gln Gly Ala Gln Phe Val Ser Tyr Asp Pro Val Asn

965 970 975

Gly Glu Trp Thr Phe Lys Val Glu His Phe Ser Ser Tyr Lys Leu Gly

980 985 990

Asp Glu Tyr Asp Val

995

<210> 3

<211> 3126

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

atgtttggct catctaatcc ttttggacag tcatctggta ccagcccatt tgggtctcaa 60

tctttgtttg gtcagaccag caatacaagt agtaataatc cctttgctcc agctacaccg 120

tttggcacgt ctgctccttt tgctgcacag tcgggaagtt caatattcgg aagcacttca 180

accggtgttt ttggtgcacc tcaaacatcc tctccctttg cctccacccc aacttttgga 240

gcttcttcat caccagcatt tgggaattct actccggcct ttggagcatc tccagcatct 300

tcaccttttg gtggatcttc tggttttggg cagaaacctt tgggattttc aacgcctcag 360

tcaaatcctt ttggaaactc cacgcagcaa tcccaacctg catttggaaa cacctctttt 420

ggttcgtcta caccttttgg tgccactaac acgcctgcct ttggcgctcc aagcacgcct 480

tcttttggcg ccacaagcac accctcgttt ggtgcatcaa gcacgcctgc ctttggtgcc 540

acaaacacgc ctgccttcgg tgcctcaaac tctccctcct ttggcgccac aaacacacct 600

gcatttggcg cgtcaccgac tccagctttt ggtagcactg gcaccacgtt tggtaacact 660

ggctttggtt ctggaggtgc ttttggagct tcaaacaccc ctgcttttgg tgcatcaggc 720

actccggctt ttggtgcatc aggcactcct gccttcggtg catcaagtac tcctgccttt 780

ggggcatcca gtactcctgc ctttggggca tccagtactc ctgcctttgg gggatctagt 840

actccttctt ttggtgcgtc aaatacctca tcctttagtt ttggctcgtc tccagctttt 900

ggccagtcta catcagcctt tggtagcagc gcctttggct ctacaccatc tccctttgga 960

ggtgcgcagg cttcaacccc tacatttggg ggttctggtt ttggtcaatc aactttcggt 1020

ggtcaacaag ggggcagtcg agctgtgcct tatgcaccaa ctgttgaggc agacacaggg 1080

accggcactc agcctgctgg aaagttggaa tctatttctg ccatgccagc atacaaagag 1140

aaaaactatg aggagctgag gtgggaagat taccagcgag gagataaagg tggaccactt 1200

cctgctggcc agtctcctgg caatgccgga tttggtatat caccttctca accaaatcca 1260

ttttctccat caccggcgtt tggtcagaca tcagcaaatc caacaaaccc tttttcgagc 1320

tcaacatcta ccaacccttt tgcccctcaa accccaacaa ttgctagttc gagttttgga 1380

acggctacct caaattttgg ttcctcgccc tttggtgtaa catcttcttc caatcttttt 1440

ggttcaggtt catcaactac cacatccgtt tttggatcat cctccgcttt tggaacaacg 1500

acaccatcac cgttatttgg ttcatcaagc actcctggat ttggctcttc ttcatcaatc 1560

tttggttcag ctcctggtca gggggcaact ccagcatttg gcaacagtca gccatctact 1620

ctgttcaatt caaccccttc gaccgggcag actggttctg cgtttggaca gactggttct 1680

gcttttggac agttcggaca gagtagtgct cctgcatttg gccaaaatag catttttaat 1740

aaaccttcca ctggatttgg aaacatgttt tccagttctt caacattaac cacgtctagc 1800

agctctccct ttggacaaac aatgcctgct ggtgtgacac cctttcaatc agctcagcca 1860

ggtcaagcat caaatggttt tggtttcaac aacttcggtc aaaatcaagc agctaatact 1920

actggaaatg ctggtggact ggggattttt ggtcaaggca acttcgggca atcgcctgct 1980

ccgctgaact ctgttgtttt gcaaccagtt gctgtgacaa acccatttgg aacacttcct 2040

gctatgcctc agatttcaat aaatcaaggt ggaaattcac cttccattca atatggaatc 2100

tccagcatgc ctgttgttga caaacctgct cctgttagaa tatcatctct cctgacttct 2160

cgacacctat tacacaggcg ggtcaggctg ccggcaagaa agtaccgtcc tggcgagaat 2220

ggtccaaagg ttccattttt cactgatgac gaagagagct caagtacacc aaaggcggat 2280

gctcttttca ttccaaggga gaaccctaga gctctagtta tacgcccagt tcaacagtgg 2340

tcttcaaggg ataaatcaat acttccaaag gaacaacgcc cgactgctcc attacatgac 2400

aatgggaaga gtcccgacat ggccactgat gcagcaaacc atgacagaaa tggtaacgga 2460

gaacttggtg ctactgggga aagaattcat acatcagtca atgcaaatca gaaaccgaat 2520

ggcacaacac gctcagatca ggctagcgag aaagagcgtc cctacaaaac tctaagtgga 2580

cacagagcag gagaagccgc aattgtttat gagcacggtg cagatatcga ggctcttatg 2640

ccgaagctac gacaatctga ttatttcaca gagccacgta tccaagaact agcagctaag 2700

gaaagagcgg atccgggtta ctgcaggcgg gtaagagact ttgtagtggg acgacacggt 2760

tatggtagca taaagttcat gggagagaca gatgtgcgta ggctagactt ggaatcactg 2820

gttcagttca atacacggga agtgattgta tacatggacg agagcaagaa accggctgtt 2880

gggcaaggtt tgaataaacc ggcggaagta acgttgctga acataaaatg tattgacaag 2940

aagacaggga aacagtttac tgaaggagag agagtggaga agtataagat gatgcttaag 3000

aagaaagctg aggcacaagg agctgagttt gtgtcatttg atccagtgaa aggcgaatgg 3060

aagtttcggg tcgagcattt tagttcgtat aagctgggcg acgaagatga agaagatgga 3120

gtttag 3126

<210> 4

<211> 2994

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

atgttcggtt cttctaataa taatcctttt ggacagtcat ctataagcag tccttttggg 60

acccaaactc attctttgtt cggacagacc aataataacg cgagcaataa tccttttgct 120

acgaaacctt ttggtacttc tactcctttt ggcgcacaga cagggagttc tatgtttggg 180

ggcacttcaa ctggtgtgtt tggtgcacct caaacttctt caccttttgg tgcttcacca 240

caagcgtttg ggagctctac tcaggctttt ggagcatctt ctaccccttc atttggcagt 300

tcaaattcac cttttggtgg cacctctacg tttggtcaga agtcttttgg cctctcaaca 360

cctcagtcaa gtccttttgg aagcaccaca caacaatcac agcctgcctt tggaaacagc 420

acttttggtt catcaacacc ttttggtgcc tcaaccacgc ctgcctttgg tgcctctagc 480

acgcctgcct ttggtgtctc aaacacttcc gggtttggag ccacaaacac acccggattt 540

ggtgccacaa acacaacagg gtttggtggc agtagcacac ctgggtttgg ggcatcaagc 600

acgcctgcct ttggctccac aaacacgcct gcttttggtg cttcaagtac tcctttattc 660

ggctcctcga gctcacctgc atttggtgcg tcacctgctc cagcctttgg tagctctggc 720

aatgcatttg gcaacaatac cttcagttct ggaggtgctt ttggttcatc aagcactcct 780

acgtttgggg catctaacac ttctgccttt ggggcatcaa gttccccatc ttttaatttc 840

ggttcttccc cagcttttgg acagtctacc tcagcatttg gtagcagttc ctttggttct 900

acacaatctt ccttaggttc tacaccatct cccttcggag cccaaggtgc acaggcttcg 960

acttcaacat ttgggggtca atcaactatc gggggtcaac aaggggggag tagggttatt 1020

ccttatgcac ctacaactga cacagcgtca ggcactgaaa gcaagtctga aagactacaa 1080

tctatatctg ccatgcctgc acacaaaggg aaaaacatgg aggagcttag gtgggaggac 1140

taccagcgag gagataaagg aggacaacgt tctactggtc aatctcctga aggtgctgga 1200

tttggtgtaa caaattctca gccaagtata ttctctacgt caccagcgtt tagccagaca 1260

cctgtgaatc caacaaaccc tttttcacag acgactccaa caagtaacac aaatttttcc 1320

ccctccttta gtcaacccac taccccatcc tttggtcaac ccactacccc atcctttagg 1380

tcaactgtat caaatactac atccgttttt ggatcatcct ctagtttaac aacaaacaca 1440

tcgcagccac ttggttcatc aatatttggt tctactcctg cacatgggtc aactccagga 1500

tttagcattg gtggatttaa caacagtcag tcatctccgc tgtttggttc aaacccctca 1560

tttgcacaaa ataccactcc tgcattcagc cagactagtc ctttgtttgg acagaatacc 1620

actcctgcat taggacagtc tagttctgtg tttggacaga ataccaatcc tgcacttgta 1680

cagagcaaca ctttcagtac accttccact ggctttggga acacgttttc aagctcttca 1740

tccttaacta caagcatctc cccctttggt caaataacgc ctgctgttac gccctttcaa 1800

tcagctcagc caactcaacc attaggtgct tttggtttca acaactttgg tcaaacccaa 1860

atagctaata caactgacat tgcaggtgca atgggaactt ttagccaagg aaactttaag 1920

caacagcctg cattgggtaa ctctgctgtt atgcaaccaa ctcctgttac aaacccattt 1980

ggaacacttc ctgctttgcc tcagatttca attgctcaag gtggaaattc accttcgatt 2040

cagtatggta tctccagcat gcctgtcgtt gataagcctg ctcctgttag agtatcacca 2100

ctcctaactt ctcgacacct tttacaaagg cgggtcaggc taccaacaag aaagtaccgt 2160

cctagtgatg atggtccaaa ggttccattc ttcagcgatg aagaggagaa ttccagtaca 2220

ccaaaggctg atgcgttttt cattccaagg gagaacccta gagctttatt tatacgccca 2280

gttgaaaggg taaaatcaga acatccaaag gatagcccga ccccactgca ggagaacggt 2340

aagaggtcga atggggttac taatggagcc aaccatgaga ctaaggataa cggtgcaatt 2400

cgtgaagctc ccccggtaaa agtcaatcag aaacagaacg ggacacatga aaatcacgga 2460

ggcgacaaaa acggttcaca cagttcacca agtggagcag atattgagtc attaatgccg 2520

aagttgcatc actctgaata tttcacagag cctcggatcc aagaactggc tgcaaaagaa 2580

agggttgaac agggttattg caagcgggta aaggactttg ttgttgggag acacggttac 2640

gggagcataa agtttctagg agagacggat gtgtgtagac tcgacctgga aatggtggtt 2700

cagttcaaaa accgggaagt gaatgtgtac atggacgaga gcaaaaaacc gccggtgggt 2760

caaggattga ataagcctgc ggtggtaact ttgctgaaca taaaatgtat ggacaagaag 2820

accggcacgc aagtgatgga aggcgagaga ttggacaagt acaaggaaat gctcaagagg 2880

aaagctgggg aacaaggagc tcagtttgtg tcttatgatc ctgtgaatgg cgagtggact 2940

ttcaaggtcg aacatttcag ttcctacaag cttggagatg aatatgatgt gtag 2994

<210> 5

<211> 23

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

atgtttggct catctaatcc ttt 23

<210> 6

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

ctaaactcca tcttcttcat cttc 24

<210> 7

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

atgttcggtt cttctaataa taat 24

<210> 8

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

ctacacatca tattcatctc caag 24

Claims (5)

1. The application of the arabidopsis thaliana nucleoporins Nup98a and Nup98b in shortening the flowering time of arabidopsis thaliana is disclosed, wherein the amino acid sequence of Nup98a is shown as SEQ ID NO.1, and the amino acid sequence of Nup98b is shown as SEQ ID NO. 2;

the application is to lead the flowering time of arabidopsis thaliana to be advanced by inactivating nucleopore proteins Nup98a and Nup98b or reducing the expression of nucleopore proteins Nup98a and Nup98 b.

2. The application of arabidopsis thaliana nucleoporins Nup98a and Nup98b in shortening the growth cycle of arabidopsis thaliana is disclosed, wherein the amino acid sequence of Nup98a is shown as SEQ ID NO.1, and the amino acid sequence of Nup98b is shown as SEQ ID NO. 2;

the application is that the growth period of arabidopsis is shortened by inactivating nucleopore proteins Nup98a and Nup98b or reducing the expression of nucleopore proteins Nup98a and Nup98 b.

3. The application of the arabidopsis nucleoporins Nup98a and Nup98b in the genetic breeding of arabidopsis with shortened early flowering or growth period or the construction of transgenic arabidopsis with shortened early flowering or growth period is disclosed, wherein the amino acid sequence of Nup98a is shown as SEQ ID NO.1, and the amino acid sequence of Nup98b is shown as SEQ ID NO. 2.

4. The application of coding genes of arabidopsis nucleopore proteins Nup98a and Nup98b or biological materials containing the coding genes in arabidopsis genetic breeding for shortening the flowering time of arabidopsis, shortening the growth cycle of arabidopsis, and shortening the early flowering or the growth period or constructing transgenic arabidopsis with shortened early flowering or the growth period, wherein the amino acid sequence of Nup98a is shown as SEQ ID NO.1, and the amino acid sequence of Nup98b is shown as SEQ ID NO. 2;

the application is that the flowering time of arabidopsis is advanced or the growth period is shortened by inactivating the coding genes of nucleopore proteins Nup98a and Nup98b or reducing the expression of the coding genes of nucleopore proteins Nup98a and Nup98 b;

the nucleotide sequence of the coding gene of Nup98a is shown in SEQ ID NO.3, and the nucleotide sequence of the coding gene of Nup98b is shown in SEQ ID NO. 4;

the biological material is an expression cassette, a vector, a transposon, an engineering bacterium, a host cell or a transgenic cell line.

5. A method for shortening flowering time and growth cycle of Arabidopsis thaliana or constructing transgenic Arabidopsis thaliana with shortened early flowering or growth period is characterized in that the flowering time of Arabidopsis thaliana is advanced or the growth period is shortened by inactivating nucleopore proteins Nup98a and Nup98b or by reducing expression of nucleopore proteins Nup98a and Nup98 b;

the amino acid sequence of Nup98a is shown in SEQ ID NO.1, and the amino acid sequence of Nup98b is shown in SEQ ID NO. 2.

Images