CN109984061A - A kind of octopus oosperm artificial incubation method - Google Patents
A kind of octopus oosperm artificial incubation method Download PDFInfo
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- CN109984061A CN109984061A CN201910255512.5A CN201910255512A CN109984061A CN 109984061 A CN109984061 A CN 109984061A CN 201910255512 A CN201910255512 A CN 201910255512A CN 109984061 A CN109984061 A CN 109984061A
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- octopus
- fertilized eggs
- oosperm
- female
- water
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- 241000238413 Octopus Species 0.000 title claims abstract description 49
- 238000011534 incubation Methods 0.000 title claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 79
- 235000013601 eggs Nutrition 0.000 claims abstract description 65
- 230000012447 hatching Effects 0.000 claims abstract description 33
- 210000004681 ovum Anatomy 0.000 claims abstract description 24
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 22
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 22
- 239000013535 sea water Substances 0.000 claims abstract description 17
- 238000011161 development Methods 0.000 claims abstract description 13
- 230000018109 developmental process Effects 0.000 claims abstract description 13
- 230000017448 oviposition Effects 0.000 claims abstract description 12
- 230000013011 mating Effects 0.000 claims abstract description 8
- 239000004568 cement Substances 0.000 claims abstract description 5
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 4
- 230000019771 cognition Effects 0.000 claims abstract description 4
- 238000005057 refrigeration Methods 0.000 claims description 17
- 238000001914 filtration Methods 0.000 claims description 11
- 230000008859 change Effects 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000004575 stone Substances 0.000 claims description 9
- 210000001161 mammalian embryo Anatomy 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 230000006399 behavior Effects 0.000 claims description 5
- 230000013020 embryo development Effects 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 3
- 241000514719 Mactra quadrangularis Species 0.000 claims description 3
- 229920000153 Povidone-iodine Polymers 0.000 claims description 3
- 241000620877 Ruditapes philippinarum Species 0.000 claims description 3
- 230000000877 morphologic effect Effects 0.000 claims description 3
- 230000003534 oscillatory effect Effects 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229960001621 povidone-iodine Drugs 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 claims description 2
- 238000000879 optical micrograph Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 238000006213 oxygenation reaction Methods 0.000 claims 1
- 239000004576 sand Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 16
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000238366 Cephalopoda Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000009287 sand filtration Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000238449 Octopoda Species 0.000 description 1
- 241000238415 Octopodidae Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003181 biological factor Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 230000005195 poor health Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of octopus oosperm artificial incubation methods, the following steps are included: (1) parent temporarily supports, lays eggs and the collection of fertilized eggs: octopus parent being placed in farm's cement pit and is temporarily supported until female, male parent mates, ovum is originated in veil by female cognition；(2) hatching apparatus parameter setting: when finding female, male parent Mating, it is spare to open hatching apparatus, after finding the oviposition of female body, fertilized eggs are taken out with long tweezers, it hangs in hatching apparatus, water temperature is set as+1 DEG C of natural temperature and is oxygenated, and seawater used is nature seawater, is placed in dark surrounds and is hatched；(3) fertilized eggs management: the development condition of routine observation fertilized eggs, the seawater of replacement in every two days, quantity of exchanged water 100%, ammonia-nitrogen content < 0.2mgL in water body‑1.The present invention reduces the adverse effect that external environment hatches octopus oosperm, so that the hatching rate of fertilized eggs is greatly improved, to meet the requirement of current octopus section's animal facilityization and standardization cultivation.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of octopus oosperm artificial incubation method.
Background technique
Octopus section (Octopodidae, d'Orbigny, 1840) is under the jurisdiction of Cephalopoda (Cephalopoda), Octopodida
(Octopoda), it is generally called octopus, is Cephalopoda maximum monoid.Wherein, short octopus and long octopus are the coastal common fishery of northern China
Economic octopus kind, and Chinese octopus is Southern Coast economic seed.It is their delicious meats, full of nutrition, it is deep to be favored by the majority of consumers,
It is the staple of foreign exchange earning, industrialized development has wide space.In recent years, with the hair of ship and Fishing technology
Exhibition, marine fishery resources failure day by day, for quantity of the catch far from the market demand is met, imbalance between supply and demand is prominent.Therefore, carry out economical
Octopus class artificial breeding for resource aquaculture and is repaired, and market demand tool realistic meaning is met.
During the artificial breeding of the full history of life, female body egg laying amount and hatchability of fertile eggs be restrict octopus class factoryization and
The problem of standardization cultivation.The hatching rate of octopus oosperm, in addition to having outside the Pass with the multiplication characteristic of animal itself, also with temperature,
The environmental factors such as salinity, dissolved oxygen, illumination and natural enemy predation are closely related.During development of fertilized ova, female body with funnel not
It is disconnected to spray water towards ovum, while ovum is stroked with sucker, protect ovum not influenced by extraneous bacterium, sundries etc.；In the female body body of development later stage
Weakness, most of individual are die.Accompanying and attending to for female body is lacked, fertilized eggs are easier to be influenced by external environment, this is not only reduced
The hatching efficiencies of fertilized eggs, while also limiting the scale of adult cultivation.
102669021 A of patent of invention CN discloses a kind of method and equipment therefor that long octopus temporarily supports, lays eggs and protect ovum,
The method includes the 1) selection of parent, 2) parent temporarily supports accelerating and 3) parent shield ovum step；And using special designing
Octopus nest.The method of the invention is easy to operate, and equipment therefor is at low cost, utilization rate is high, can effectively improve long octopus parent survival rate,
The health degree of the hatching rate and seedling of egg laying amount and fertilized eggs.The inventive method only describes the temporary of long octopus under natural conditions
It supports, the method and equipment therefor of oviposition and shield ovum, does not specify method for incubating oosperm and device under artificial condition.It is sent out in fertilized eggs
It educates the later period, the female octopus stopping of shield ovum is ingested, and in poor health, most of individual is die.Lack accompanying and attending to for female body, fertilized eggs be easier by
To the influence of external environment, this not only lowers the hatching efficiencies of fertilized eggs, while also limiting the scale of adult cultivation.
Summary of the invention
In view of the deficiencies of the prior art, the present invention is existing to solve by providing a kind of octopus oosperm artificial incubation method
Have under octopus oosperm hatching pattern due to the environmental factors such as temperature, salinity, dissolved oxygen, illumination and with natural enemy predation, female body it is dead
The inferior technical problem of incubating oosperm low efficiency caused by biological factors such as die.
To achieve the above object, the technical solution of the present invention is as follows:
A kind of octopus oosperm artificial incubation method, comprising the following steps:
(1) parent temporarily support, lay eggs and fertilized eggs collection:
In breeding period, reach sexually matured octopus in natural waters capture, chooses the complete individual of wherein health as parent
Body, parent, which is placed in farm's cement pit, temporarily supports until female, male parent mating, after the completion of female, male parent mating, male body is successive
It dies, female body enters veil and prepares oviposition, and after a period of time, ovum is originated in veil by female cognition；
(2) hatching apparatus parameter setting:
When finding female, male parent Mating, unlatching hatching apparatus is spare, after finding the oviposition of female body, with long tweezers
Fertilized eggs are taken out, are hung in hatching apparatus, water temperature is set as+1 DEG C of natural temperature and is oxygenated, and seawater used is nature sea
Water is placed in dark surrounds and is hatched；
(3) fertilized eggs management:
The development condition of routine observation fertilized eggs, the seawater of replacement in every two days, quantity of exchanged water 100%, ammonia nitrogen in water body
Content < 0.2mgL-1。
Preferably, first poly- with 0.2-0.5ppm in the octopus as parent that natural waters fish in step (1)
Dimension ketone iodine carries out disinfection processing, then places into aquaculture pond and temporarily supports；Temporarily the quantity ratio of feeding female, male parent is 1:1.
Preferably, in step (1), when parent is placed in farm's cement pit temporarily feeding, with Mactra veneriformis or Ruditapes philippinarum
As bait；Using terrine as veil and spawning place；During temporarily supporting, residual bait should be removed in time, replaces fresh bait；Female body
When preparing to lay eggs into veil, the food ration of female body is less, can suitably reduce bait feeding；After female body abstains from food in whole, clearly
Except residual bait, fresh bait is no longer added.
Preferably, the water temperature in step (1) is set as same day nature seawater water temperature, and using electronic circulation refrigeration machine control temperature
Degree；And it is normally inflated, dissolved oxygen content > 5mgL-1；Intensity of illumination < 100Lux；Every 1-2 days to change water primary.
Further, in step (2), the hatching apparatus includes water quality purifying box, incubator, circulating water pipe I and recirculated water
Pipe II is equipped with electronic circulation refrigeration machine and filter in the water quality purifying box, be equipped in the incubator filtration cycle water pump,
For hanging the hanger bar of fertilized eggs to be hatched and being placed in the gas stone of incubator bottom；The one end the circulating water pipe I and electricity
Subcycle refrigeration machine is connected, and the other end is inserted into incubator；The side of the circulating water pipe II is connected with filter, the other end
It is connected with filtration cycle water pump.
Preferably, in step (2), the filter is located at close to the side of incubator, electronic circulation refrigeration seat in the plane
In the side far from incubator；The filtration cycle water pump is located at close to the side of water quality purifying box；The one end the circulating water pipe I
It is connected with electronic circulation refrigeration machine, the other end is inserted into the side in incubator far from water quality purifying box；The hanger bar is more
Root is arranged in parallel along water (flow) direction；The gas stone is multiple.
Preferably, in step (2), in the hatching process of ovum, water temperature is set as+1 DEG C of natural temperature, and uses electronic circulation
Refrigeration machine controls temperature；Nature seawater used need to be after disinfection to reserve through sand filtration, precipitating, UV.
Preferably, it in step (2), is oxygenated using gas stone, makes dissolved oxygen content > 5mgL-1, while adjusting air-flow size
With the flow velocity of recirculated water, allow fertilized eggs slight oscillatory in water, to simulate female body shield ovum behavior.
Preferably, in step (3), in order to understand the development condition of fertilized eggs accurately and in time, every 3-5 days, every time with
Machine extracts 5-10 fertilized eggs；When sampling, single fertilized eggs are removed from ovum fringe with the scissors of plastics, tweezers, are put in culture
In ware, it is placed in developmental stage, the morphological feature of optical microphotograph microscopic observation embryo；Scissors used, tweezers must be sterilized.
Preferably, in step (3), in order to guarantee the consistency of incubating oosperm rate, when observation reaches embryonic development
When phase is inconsistent, by adjusting incubation temperature, change the developmental rate of fertilized eggs: when developmental rate is very fast, appropriate reduce is incubated
Change temperature；When deformity occur in the fertilized eggs of observation or embryo morphology, incubation temperature should be suitably reduced.
Compared with prior art, advantages of the present invention:
(1) present invention indicates critical problem present in octopus oosperm collection, hatching process, sets without complicated
It applies, instrument and equipment and operating instruction, strong operability.
(2) the present invention provides a kind of devices of incubation condition needed for accurate control octopus oosperm, by fertilized eggs
Growth course is placed in scientific, controllable range, effectively improves the hatching efficiency of octopus oosperm, to recovery natural resources
It is significant with the market demand is met.
(3) present invention protects ovum behavior by the female body of manual simulation, can effectively improve the hatching efficiency of fertilized eggs, and operate letter
Just, easy to spread.
(4) the present invention provides a kind of method and Testing index for detecting development of fertilized ova situation, easy to operate, practicabilities
By force, the development condition of fertilized eggs can be understood accurately and in time.
(5) the present invention provides a kind of method for controlling incubating oosperm rate, the hatching rate of fertilized eggs is made to be placed in people
For in control range, while the aberration rate of fertilized eggs can be reduced.
In conclusion reducing external environment using hatching apparatus and hatching method of the invention and hatching to octopus oosperm
Adverse effect meet current octopus section's animal facilityization and standardization cultivation to greatly improve the hatching efficiency of fertilized eggs
It is required that.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is for the overall structure diagram of octopus oosperm artificial incubation device of the invention；
Fig. 2 is the course of work schematic diagram of the device；
Appended drawing reference: A1, water quality purifying box；A2, electronic circulation refrigeration machine；A3, filter；B1, incubator；B2, filtering
Water circulating pump；B3, hanger bar；B4, fertilized eggs；B5, gas stone；C1, circulating water pipe I；C2, circulating water pipe II.
Specific embodiment
The present invention is capable of providing a kind of octopus oosperm artificial incubation device and artificial incubation method, by temperature, salinity,
The environment condition controls such as dissolved oxygen, illumination within the appropriate range, while also making fertilized eggs in growth course from natural enemy
Infringement.This method simulates female body and protects ovum behavior, solves in its natural state, fertilized eggs can not be incubated normally due to losing female body and accompanying and attending to
The key techniques such as change, it is ensured that large-scale artificial breeding is gone on smoothly with cultivation, has great industrial value.
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Embodiment 1
As shown in Figs. 1-2, a kind of octopus oosperm artificial incubation device, including water quality purifying box (A1), incubator (B1),
Circulating water pipe I (C1) and circulating water pipe II (C2).Filter (A3) and electronic circulation system are equipped in the water quality purifying box (A1)
Cold (A2), the filter (A3) are located at close to the side of incubator (B1), and the electronic circulation refrigeration machine (A2) is located at remote
Side from incubator (B1).
The length of the incubator (B1) is 50cm, and the height of water level in the incubator (B1) is controlled in 30cm or so,
The water surface in the incubator (B1) did not had the top of fertilized eggs (B4).Filtration cycle water pump is equipped in the incubator (B1)
(B2), the gas stone (B5) for hanging the hanger bar (B3) of fertilized eggs to be hatched (B4) and being placed in the bottom incubator (B1),
The filtration cycle water pump (B2) is located at close to the side of water quality purifying box (A1)；The hanger bar (B3) is more, along water flow
Direction is arranged in parallel；The gas stone (B5) is multiple groups, and every group to be multiple, is arranged along the hanger bar direction (B3).The electronic circulation
The voltage specification of refrigeration machine (A2) is AC110-240V/50-60Hz, and temperature setting range is 10-40 DEG C, water flow 1-3L/
Min, the water pipe (i.e. circulating water pipe I (C1)) suitable for internal diameter 8mm.
Described one end (C1) circulating water pipe I is connected with electronic circulation refrigeration machine (A2), and the other end is inserted into incubator (B1)
The side of interior separate water quality purifying box (A1).The side of the circulating water pipe II (C2) is connected with filter (A3), the other end with
Filtration cycle water pump (B2) is connected.
Embodiment 2
A kind of octopus oosperm artificial incubation method, comprising the following steps:
(1) parent temporarily support, lay eggs and fertilized eggs collection
In annual breeding period in 4-5 month, reaches sexually matured octopus in natural waters capture, it is complete to choose wherein health
Individual is used as parent, is first carried out disinfection processing with 0.2-0.5ppm povidone iodine, then places into aquaculture pond and temporarily support, female, male
The quantity ratio of parent is 1:1.In the parent of field capture, it is necessary to by stringent screening, povidone iodine disinfection treatment, prevent bacterium
Infection causes parent massive mortality.
Using terrine as veil and spawning place.Using Mactra veneriformis or Ruditapes philippinarum as bait, remove in time residual
Bait replaces fresh bait；When female body enters veil preparation oviposition, the food ration of female body is less, can suitably reduce bait feeding；
After female body abstains from food in whole, residual bait is removed, fresh bait is no longer added.Water temperature is set as same day nature seawater water temperature, and using electricity
Subcycle refrigeration machine controls temperature；Normal inflation, dissolved oxygen content answer > 5mgL-1；Intensity of illumination answers < 100Lux；It is 1-2 days every
It is primary to change water.
After the completion of female, male parent mating, male body is died off, and female body, which enters, prepares oviposition in terrine, after 10-15 days,
Ovum is originated in veil (i.e. terrine) by female cognition.
(2) hatching apparatus parameter setting
Hatching apparatus uses the octopus oosperm artificial incubation device in embodiment 1.
When finding female, male parent Mating, it is spare to open hatching apparatus.After finding the oviposition of female body, with long tweezers
Fertilized eggs are taken out, are hung in hatching apparatus with nylon rope.Water temperature is set as+1 DEG C of natural temperature, is freezed using electronic circulation
Machine controls temperature, and embryonic development speed can suitably be accelerated by adopting this method, and type longer for development time can be improved
The hatching rate of fertilized eggs.
It is oxygenated using gas stone, makes dissolved oxygen content > 5mgL-1, while adjusting the stream of air-flow size and recirculated water
Speed allows fertilized eggs slight oscillatory in water, to simulate female body shield ovum behavior.Seawater used is nature seawater, will be natural
Seawater need to be after disinfection to reserve through sand filtration, precipitating, UV, to reduce influence of the microorganisms such as bacterium, fungi to fertilized eggs.It is placed in black
Hatched in dark situation.
(3) fertilized eggs management:
For the hatching quality for guaranteeing fertilized eggs, the development condition of routine observation fertilized eggs is needed.It is every 3-5 days, random every time to take out
5-10 fertilized eggs are taken, single fertilized eggs need to be removed with scissors, tweezers from ovum fringe, be put in culture dish, it is aobvious to be placed in body formula
The developmental stage of micro- microscopic observation embryo, morphological feature, scissors used, tweezers must be sterilized.In order to guarantee incubating oosperm speed
The consistency of rate, when observation reach embryonic development period it is inconsistent when, by adjusting incubation temperature, change the development speed of fertilized eggs
Rate: when developmental rate is very fast, incubation temperature is suitably reduced；Temperature is excessively high may cause fertilized eggs or embryo form occur it is abnormal
Shape should suitably reduce incubation temperature when the fertilized eggs of observation or embryo morphology appearance deformity.The primary sea of replacement in every two days
Water, quantity of exchanged water 100%, ammonia-nitrogen content < 0.2mgL in water body-1。
Under field conditions (factors), the incubating oosperm time is 30-40 days；Using methods and apparatus of the present invention, in hatching water
At 18 DEG C, 21 DEG C and 24 DEG C of temperature, the brooding time of fertilized eggs is respectively 40 days, 30 days and 24 days.When incubation temperature is 18 DEG C,
Brooding time will be considerably less than under natural conditions；And incubation condition can be adjusted according to production requirement, to change fertilized eggs
Brooding time.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. a kind of octopus oosperm artificial incubation method, which comprises the following steps:
(1) parent temporarily support, lay eggs and fertilized eggs collection:
In breeding period, reach sexually matured octopus in natural waters capture, chooses the complete individual of wherein health and be used as parent, parent
Body, which is placed in farm's cement pit, temporarily supports until female, male parent mates, and after the completion of female, male parent mating, male body is died off,
Female body enters veil and prepares oviposition, and after a period of time, ovum is originated in veil by female cognition；
(2) hatching apparatus parameter setting:
When finding female, male parent Mating, it is spare to open hatching apparatus, will be by with long tweezers after finding the oviposition of female body
Smart ovum takes out, and hangs in hatching apparatus, and water temperature is set as+1 DEG C of natural temperature and is oxygenated, and seawater used is nature seawater, sets
Hatched in dark surrounds；
(3) fertilized eggs management:
The development condition of routine observation fertilized eggs, the seawater of replacement in every two days, quantity of exchanged water 100%, ammonia-nitrogen content in water body
< 0.2mgL-1。
2. octopus oosperm artificial incubation method according to claim 1, it is characterised in that: in step (1), in natural sea
The octopus as parent that area fishes first is carried out disinfection processing with 0.2-0.5ppm povidone iodine, then places into cultivation
It is temporarily supported in pond；Temporarily the quantity ratio of feeding female, male parent is 1:1.
3. octopus oosperm artificial incubation method according to claim 1, it is characterised in that: in step (1), parent is placed on
When temporarily being supported in farm's cement pit, using Mactra veneriformis or Ruditapes philippinarum as bait；Using terrine as veil and spawning ground
Institute；During temporarily supporting, residual bait should be removed in time, replaces fresh bait；When female body enters veil preparation oviposition, the food ration of female body
It is less, it can suitably reduce bait feeding；After female body abstains from food in whole, residual bait is removed, fresh bait is no longer added.
4. octopus oosperm artificial incubation method according to claim 1, it is characterised in that: the water temperature in step (1) is set
For same day nature seawater water temperature, and temperature is controlled using electronic circulation refrigeration machine；And normally inflated, dissolved oxygen content answers >
5mg·L-1；Intensity of illumination answers < 100Lux；Every 1-2 days to change water primary.
5. octopus oosperm artificial incubation method according to claim 1-4, it is characterised in that: in step (2),
The hatching apparatus includes water quality purifying box, incubator, circulating water pipe I and circulating water pipe II, is equipped in the water quality purifying box
Electronic circulation refrigeration machine and filter, the incubator is interior to be equipped with filtration cycle water pump, for hanging the outstanding of fertilized eggs to be hatched
Hanging rod and the gas stone for being placed in incubator bottom；The one end the circulating water pipe I is connected with electronic circulation refrigeration machine, and the other end is inserted
Enter to incubator；The side of the circulating water pipe II is connected with filter, and the other end is connected with filtration cycle water pump.
6. octopus oosperm artificial incubation method according to claim 5, it is characterised in that: in step (2), the filtering
Device is located at close to the side of incubator, and the electronic circulation refrigeration machine is located remotely from the side of incubator；The filtration cycle water
Pump is located at close to the side of water quality purifying box；The one end the circulating water pipe I is connected with electronic circulation refrigeration machine, and the other end is inserted into
Far from the side of water quality purifying box in incubator；The hanger bar is more, is arranged in parallel along water (flow) direction；The gas Shi Weiduo
It is a.
7. octopus oosperm artificial incubation method according to claim 5, it is characterised in that: in step (2), in incubating for ovum
During change, water temperature is set as+1 DEG C of natural temperature, and controls temperature using electronic circulation refrigeration machine；Nature seawater used need to be through sand
Filter, precipitating, UV are after disinfection to reserve.
8. octopus oosperm artificial incubation method according to claim 5, it is characterised in that: in step (2), utilize gas stone
Oxygenation, makes dissolved oxygen content > 5mgL-1, while the flow velocity of air-flow size and recirculated water is adjusted, allow fertilized eggs in water
Slight oscillatory, to simulate female body shield ovum behavior.
9. octopus oosperm artificial incubation method according to claim 5, it is characterised in that: in step (3), in order to and
When, accurately understand fertilized eggs development condition, every 3-5 days, randomly select 5-10 fertilized eggs every time；When sampling, plastics are used
Scissors, tweezers single fertilized eggs are removed from ovum fringe, be put in culture dish, be placed in the hair of optical microphotograph microscopic observation embryo
Educate period, morphological feature；Plastics scissors used, tweezers must be sterilized.
10. octopus oosperm artificial incubation method according to claim 5, it is characterised in that: in step (3), in order to protect
The consistency for demonstrate,proving incubating oosperm rate, when observation reach embryonic development period it is inconsistent when, by adjusting incubation temperature, change
The developmental rate of fertilized eggs: when developmental rate is very fast, incubation temperature is suitably reduced；When the fertilized eggs or embryo morphology of observation
When there is deformity, incubation temperature should be suitably reduced.
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| CN117958186A (en) * | 2024-04-01 | 2024-05-03 | 水利部交通运输部国家能源局南京水利科学研究院 | Method for determining water temperature process in ecological flow process of fish full life history habitat requirements |
| CN117958186B (en) * | 2024-04-01 | 2024-06-21 | 水利部交通运输部国家能源局南京水利科学研究院 | Method for determining water temperature process in ecological flow process of fish full life history habitat requirements |
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