CN109984043A - It is a kind of using blade as the camphor tree leaf blueberry method for tissue culture of explant - Google Patents
It is a kind of using blade as the camphor tree leaf blueberry method for tissue culture of explant Download PDFInfo
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- CN109984043A CN109984043A CN201910403753.XA CN201910403753A CN109984043A CN 109984043 A CN109984043 A CN 109984043A CN 201910403753 A CN201910403753 A CN 201910403753A CN 109984043 A CN109984043 A CN 109984043A
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- 241000723346 Cinnamomum camphora Species 0.000 title claims abstract description 61
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 60
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 60
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 60
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000006698 induction Effects 0.000 claims abstract description 50
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 238000005286 illumination Methods 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 4
- 238000006748 scratching Methods 0.000 claims description 4
- 230000002393 scratching effect Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000000465 moulding Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 241000208421 Ericaceae Species 0.000 description 2
- 241000287127 Passeridae Species 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 235000012511 Vaccinium Nutrition 0.000 description 2
- 241000736767 Vaccinium Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000763 evoking effect Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 208000006820 Arthralgia Diseases 0.000 description 1
- OONDLKCAZJZRCW-QNDFHXLGSA-N Caffeoyl-arbutin Natural products C([C@@H]1[C@H]([C@@H]([C@@H](O)[C@H](OC=2C=CC(O)=CC=2)O1)O)O)OC(=O)C=CC1=CC=C(O)C(O)=C1 OONDLKCAZJZRCW-QNDFHXLGSA-N 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 241000733416 Vaccinium dunalianum Species 0.000 description 1
- 235000002818 Vaccinium dunalianum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009740 moulding (composite fabrication) Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of using blade as the camphor tree leaf blueberry method for tissue culture of explant, the following steps are included: step 1: the induction of blade callus, take the camphor tree leaf blueberry tissue-cultured seedling of blade stalwartness, scuffing processing rear blade is carried out in blade surface and is placed in media surface, and culture medium is added and carries out callus induction；Step 2: callus is induced molding seedling to be transferred in adventitious bud induction culture base and carries out adventitious bud induction culture by adventitious bud induction culture；Step 3: rooting induction, the healthy and strong camphor tree leaf blueberry tissue culture plant inoculation that adventitious bud induction culture is grown up to are cultivated in root media, carry out root induction, obtain camphor tree leaf blueberry rooted seedling.The method of the present invention explant inductivity is up to 86.7%, and the adventitious bud seedling effect of induction is good, and seedling is sturdy, and blade is full；Seedling produces a large amount of root after rooting induction, and the survival rate of robust growth, transplanting is higher.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of using blade as the camphor tree leaf blueberry group of explant
Knit cultural method.
Background technique
Camphor tree leaf blueberry (Vaccinium dunalianum) is Ericaceae (Ericaceae) genus vaccinium (Vaccinium)
Plant, evergreen shrubs.Main product is in the ground such as Yunnan, Sichuan, Guizhou, Tibet, China Taiwan and Sillim, Bhutan, India (northeast
Portion), Burma (northeast) be also distributed to Vietnam, be then distributed mainly on northwestern Yunnan Province within the border through Center Yunnan Plateau extremely in Yunnan Province
The southern regions of the Yunnan Province and South-east of Yunnan.Its young tender leaf bud, it is civil also known as " sparrow mouth tea " in Yi nationality of Yunnan because likeness in form sparrow mouth is tapered, according to records certainly
The Ming Dynasty starts just to grow tea substitute long-term drinking so far as one.Camphor tree leaf blueberry (former mutation) complete stool is pharmaceutically acceptable, is used for rheumatism
Arthralgia, 6 '-O- caffeoyl arbutin CA of main component are widely used as the skin-whitening natural agents raw material of cosmetic industry.
But wild plant resource gradually decreases, artificial cultivation excessive cycle, slow growth, it is also possible to since ecological environment diversity is poor
There are pest and disease damage harm, lead to the decline of its effective component yield, quality variation.
It is very few to the report of camphor tree leaf blueberry evoked callus, adventitious bud acquisition secondary metabolite both at home and abroad at present, because
The Plant Tissue Breeding research of this expansion camphor tree leaf blueberry is very necessary, has and supplements its insufficient effect manually cultivated.
Summary of the invention
It is an object of the invention to a kind of get over by the camphor tree leaf of explant of blade in view of the deficienciess of the prior art, providing
Tangerine method for tissue culture.Camphor tree leaf blueberry blade adventitious bud induction frequency reaches 86.7% under the cultural method, and this method has explant
Body material easily obtains, generates the advantages that clump bud amount is more, reproduction speed is fast, the period is short, is to obtain a large amount of camphor tree leaf blueberry childrens in a short time
The effective way of seedling.
To achieve the purpose of the present invention, herein provided by using blade as the camphor tree leaf blueberry method for tissue culture of explant with
The blade of camphor tree leaf blueberry is that explant progress camphor tree leaf blueberry tissue-cultured seedling is cultivated, comprising the following steps:
Step 1: the induction of blade callus takes the camphor tree leaf blueberry tissue-cultured seedling of blade stalwartness, carries out scuffing processing in blade surface
Rear blade is placed in calli induction media surface and carries out callus induction；
Step 2: adventitious bud induction culture, the seedling that callus is induced are transferred to adventitious bud in adventitious bud induction culture base
Fiber differentiation；
Step 3: rooting induction, by healthy and strong camphor tree leaf blueberry tissue culture plant inoculation that adventitious bud induction culture grows up in taking root
It is cultivated in culture medium, carries out root induction, obtain camphor tree leaf blueberry rooted seedling.
Further, the culture medium in the step 1 are as follows: WPM+NAA0.01~0.1mg/L+TDZ0.1~1.5mg/L,
PH5.8。
Further, the condition of culture of the step 1 are as follows: 23 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination,
Periodicity of illumination ratio 12h:12.
Further, adventitious bud induction culture base in the step 2 are as follows: WPM+0.3mg/L ZT, PH5.8.
Further, the condition of culture of the step 2 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination,
Periodicity of illumination ratio 12h:12h.
Further, the root media in the step 3 are as follows: 0.5~3.0mg/L+NAA0.1 of WMP+6-BA~
1.0mg/L。
Further, the condition of culture of the step 3 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination,
Periodicity of illumination ratio 12h:12h.
Further, the pH value of culture medium is adjusted with 1mol/L NaOH or HCl.
Further, scuffing processing is carried out in the lower surface of camphor tree leaf blueberry blade in the step 1.
Further, the lower surface through scratching processing is flat on media surface.
Compared with prior art, superiority of the invention is:
(1) using the blade of camphor tree leaf blueberry tissue-cultured seedling as explant；
(2) drug price used in is cheap, at low cost；
(3) the method have the characteristics that hormone combination is simple, using WPM as minimal medium, with NAA, TDZ, ZT, 6-BA couple
Adventitious bud inducing and proliferation, the influence taken root are analyzed camphor tree leaf blueberry blade adventitious bud inducing to obtain inducer blade production
Raw adventitious bud is to taking root；
(4) the method for the present invention explant inductivity is up to 86.7%, and the adventitious bud seedling effect of induction is good, and seedling is sturdy, leaf
Piece is full；Seedling produces a large amount of root after rooting induction, and the survival rate of robust growth, transplanting is higher.
Detailed description of the invention
Fig. 1 is that camphor tree leaf blueberry blade of the present invention is cut into the rectangular of length and width about 1cm, and scratch to surface the signal of processing
Figure；
Fig. 2 is the present invention (WPM+0.05mgL-1NAA+1.5mg·L-1TZD camphor tree leaf blueberry blade callus induction group) is organized
Knit schematic diagram；
Fig. 3 is the present invention (WPM+0.05mgL-1NAA+1.5mg·L-1TZD) group camphor tree leaf blueberry Callus of Leaf lures
Lead adventitious bud schematic diagram；
Fig. 4 is that (WPM+0.3mg/L ZT) of the invention organizes camphor tree leaf blueberry adventitious bud inducing into seedling schematic diagram；
Fig. 5 is that (WMP+6-BA 1.5mg/L+NAA 0.5mg/L) of the invention organizes camphor tree leaf blueberry seedling root induction signal
Figure；
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing be the present invention do into
The detailed description of one step passes through the following examples further illustrate the present invention in detail herein, but the contents of the present invention are simultaneously
It is not limited to this.
As shown in Figs. 1-5, the present invention is provided herein the camphor tree leaf blueberry method for tissue culture of explant with camphor tree using blade
The blade of leaf blueberry is that explant progress camphor tree leaf blueberry tissue-cultured seedling is cultivated, comprising the following steps:
Step 1: the induction of blade callus takes the camphor tree leaf blueberry tissue-cultured seedling for growing up to the blade stalwartness of 5cm or so, in blade table
Face carries out scuffing processing rear blade and is placed in media surface, as shown in Figure 1, carrying out callus induction, as shown in Figure 2；It can basis
Fostering requirement carries out multiple groups culture simultaneously, such as 2 groups of culture or more, and every group 5 bottles, every bottle of 6, inoculation blade；It uses in this step
Culture medium using WPM as minimal medium, be added hormone, and adjust the pH of the pH of culture medium after sterilization be 5.8；What is be added swashs
Element is the concentration of NAA and TDZ, NAA and TDZ according to culture situation setting, uses NAA0.01mg/L~0.1mg/L herein, specifically
For 0.01mg/L, 0.05mg/L or 0.1mg/L；TDZ0.1~1.5mg/L, specially 0.1mg/L, 0.5mg/L, 0.6mg/L,
0.8mg/L, 1.0mg/L or 1.5mg/L；In order to better ensure that camphor tree leaf blueberry blade adventitious bud induction frequency reaches 86.7%, adopt
Calli induction media are as follows: 0.05mg/L NAA+1.5mg/L TDZ+WPM makes callus fast growing and vigorous, with big
Amount new plant is grown, and growing state is best, and color is greener；There is bud point, the adventitious bud seedling effect of induction is good, and seedling is sturdy, leaf
Piece is full；
Step 2: adventitious bud induction culture, callus induction carry out 40 angel's blade formings of induction, molding seedling are transferred to
Adventitious bud induction culture is carried out in adventitious bud induction culture base；Adventitious bud induction culture base are as follows: WPM+0.3mg/L ZT, PH5.8；
Using the Callus of Leaf evoking adventive bud of camphor tree leaf blueberry, and then plant regeneration, establish regenerating system；The molding children of step 1
It after seedling grows 40d or so on adventitious bud induction culture base, can grow to 3-5cm, there is 4-8 piece leaf, the robust plant grown up to, leaf
Piece is dark green, grows fine；
Step 3: rooting induction, by healthy and strong camphor tree leaf blueberry tissue culture plant inoculation that adventitious bud induction culture grows up in taking root
It is cultivated in culture medium, carries out root induction, obtain camphor tree leaf blueberry rooted seedling, as shown in Figure 5；Culture of rootage used herein
Base are as follows: WMP+6-BA 0.5~3.0mg/L+NAA, 0.1~1.0mg/L according to circumstances sets the culture of rootage time, generally
30d, seedling produce a large amount of root after rooting induction, and the survival rate of robust growth, transplanting is higher.
In step 1 when carrying out blade scuffing processing, need to remove blade tip, petiole with scalpel in aseptic operating platform
It is organized with leaf two sides, obtains the rectangular blade of length and width about 1cm or so.
The condition of culture of step 1 are as follows: 23 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio
12h:12；The condition of culture of step 2 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio 12h:
12h；The condition of culture of step 3 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio 12h:
12h。
Here, the present invention adjusts pH value with 1mol/L NaOH or HCl.
In order to preferably induce camphor tree leaf blueberry, when carrying out scratching processing to camphor tree leaf blueberry, the present invention is in camphor tree leaf
The lower surface of blueberry blade carries out scuffing processing, and the lower surface through scratching processing is flat on media surface, ensure that and lures
The success rate led.
The present invention, using blade as explant, is advanced optimized using the camphor tree leaf blueberry tissue-cultured seedling of tissue cultures as material
The breeding condition of camphor tree leaf blueberry, widens the Regeneration Ways of its adventitious bud, and it is more indefinite to expect to generate using less explant
Bud accelerates the breeding cycle of camphor tree leaf blueberry to a certain extent.It is the transgenic technology of camphor tree leaf blueberry after establishing this tissue culture system
Basis early period is provided, convenient for target gene to be transferred in camphor tree leaf blueberry from callus, lays the foundation to obtain high-content CA, is
The further comprehensive exploitation of the plant resources of camphor tree leaf blueberry and constituents extraction utilize offer theoretical reference.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, those of ordinary skill in the art are to this hair
The modification or equivalent replacement that bright technical solution is made are contained without departing from the spirit and scope of technical solution of the present invention
It covers in scope of the presently claimed invention.
Claims (10)
1. a kind of using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: this method is with camphor tree leaf blueberry
Blade is that explant progress camphor tree leaf blueberry tissue-cultured seedling is cultivated, comprising the following steps:
Step 1: the induction of blade callus takes the camphor tree leaf blueberry tissue-cultured seedling of blade stalwartness, the leaf after blade surface carries out scuffing processing
Piece is placed in calli induction media surface and carries out callus induction；
Step 2: adventitious bud induction culture, the seedling that callus is induced are transferred to adventitious bud inducing in adventitious bud induction culture base
Culture；
Step 3: rooting induction, the healthy and strong camphor tree leaf blueberry tissue culture plant inoculation that adventitious bud induction culture is grown up to is in culture of rootage
It is cultivated in base, carries out root induction, obtain camphor tree leaf blueberry rooted seedling.
2. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
Calli induction media in step 1 are as follows: WPM+NAA0.01~0.1mg/L+TDZ0.1~1.5mg/L, PH5.8.
3. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
The condition of culture of step 1 are as follows: 23 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio 12h:12.
4. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
Adventitious bud induction culture base in step 2 are as follows: WPM+0.3mg/L ZT, PH5.8.
5. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
The condition of culture of step 2 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio 12h:12h.
6. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
Root media in step 3 are as follows: 0.5~3.0mg/L+NAA of WMP+6-BA, 0.1~1.0mg/L.
7. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
The condition of culture of step 3 are as follows: 25 ± 2 DEG C of cultivation temperature, 1500~2500lx of intensity of illumination, periodicity of illumination ratio 12h:12h.
8. according to claim 2 or 4 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that:
The pH value of culture medium is adjusted with 1mol/L NaOH or HCl.
9. according to claim 1 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: described
Scuffing processing is carried out in the lower surface of camphor tree leaf blueberry blade in step 1.
10. according to claim 9 using blade as the camphor tree leaf blueberry method for tissue culture of explant, it is characterised in that: will
Lower surface through scratching processing is flat on media surface.
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| CN201910403753.XA CN109984043B (en) | 2019-05-15 | 2019-05-15 | Method for culturing tissue of vaccinium camphorate by taking leaves as explants |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110622862A (en) * | 2019-10-16 | 2019-12-31 | 西南林业大学 | Method for establishing suspension cell culture system of vaccinium camphorata |
| CN111183903A (en) * | 2020-03-13 | 2020-05-22 | 西南林业大学 | Method for increasing content of 6' -O-caffeoyl arbutin in vaccinium camphorate tissue culture seedlings |
| CN112931223A (en) * | 2021-04-16 | 2021-06-11 | 山东大丰园农业有限公司 | Culture medium and culture method for blueberry tissue culture |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU1824115C (en) * | 1991-02-12 | 1993-06-30 | Центральный Ботанический Сад Ан Бсср | Method of multiplication of viccinium vitis-idaea l in vitro |
| CN103999773A (en) * | 2014-05-27 | 2014-08-27 | 昆明凌览生物科技有限公司 | Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin |
| WO2014167464A1 (en) * | 2013-04-08 | 2014-10-16 | Sederma | Production method of meristematic cells of plantago lanceolata, composition comprising said cells or their cellular extract, and cosmetic, nutraceutical and dermatological uses |
| CN104255474A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method for culture of vaccinium bracteatum suspension cells |
| CN105900843A (en) * | 2016-05-10 | 2016-08-31 | 中国科学院植物研究所 | Bog bilberry tissue culture fast breeding method and culture medium thereof |
| CN108157178A (en) * | 2018-01-09 | 2018-06-15 | 西南林业大学 | A kind of camphor tree leaf blueberry is taken root and transplants hardening off method |
| CN108770691A (en) * | 2018-05-21 | 2018-11-09 | 西南林业大学 | A method of induction camphor tree leaf blueberry tissue culture seedling leaf directly generates adventitious root |
| CN110622862A (en) * | 2019-10-16 | 2019-12-31 | 西南林业大学 | Method for establishing suspension cell culture system of vaccinium camphorata |
-
2019
- 2019-05-15 CN CN201910403753.XA patent/CN109984043B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU1824115C (en) * | 1991-02-12 | 1993-06-30 | Центральный Ботанический Сад Ан Бсср | Method of multiplication of viccinium vitis-idaea l in vitro |
| WO2014167464A1 (en) * | 2013-04-08 | 2014-10-16 | Sederma | Production method of meristematic cells of plantago lanceolata, composition comprising said cells or their cellular extract, and cosmetic, nutraceutical and dermatological uses |
| CN103999773A (en) * | 2014-05-27 | 2014-08-27 | 昆明凌览生物科技有限公司 | Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin |
| CN104255474A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method for culture of vaccinium bracteatum suspension cells |
| CN105900843A (en) * | 2016-05-10 | 2016-08-31 | 中国科学院植物研究所 | Bog bilberry tissue culture fast breeding method and culture medium thereof |
| CN108157178A (en) * | 2018-01-09 | 2018-06-15 | 西南林业大学 | A kind of camphor tree leaf blueberry is taken root and transplants hardening off method |
| CN108770691A (en) * | 2018-05-21 | 2018-11-09 | 西南林业大学 | A method of induction camphor tree leaf blueberry tissue culture seedling leaf directly generates adventitious root |
| CN110622862A (en) * | 2019-10-16 | 2019-12-31 | 西南林业大学 | Method for establishing suspension cell culture system of vaccinium camphorata |
Non-Patent Citations (4)
| Title |
|---|
| SAMIR C. DEBNATH: ""Propagation of Vaccinium in Vitro"", 《INTERNATIONAL JOURNAL OF FRUIT SCIENCE》 * |
| 付羚等: ""樟叶越桔愈伤组织诱导及其细胞悬浮培养体系建立"", 《西部林业科学》 * |
| 叶飞等: ""越桔叶片组培快繁技术研究"", 《中国南方果树》 * |
| 罗旭璐等: ""樟叶越桔的组织培养与快速繁殖"", 《植物生理学报》 * |
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| CN110622862A (en) * | 2019-10-16 | 2019-12-31 | 西南林业大学 | Method for establishing suspension cell culture system of vaccinium camphorata |
| CN110622862B (en) * | 2019-10-16 | 2022-04-19 | 西南林业大学 | Method for establishing suspension cell culture system of vaccinium camphorata |
| CN111183903A (en) * | 2020-03-13 | 2020-05-22 | 西南林业大学 | Method for increasing content of 6' -O-caffeoyl arbutin in vaccinium camphorate tissue culture seedlings |
| CN111183903B (en) * | 2020-03-13 | 2021-12-14 | 西南林业大学 | Method for increasing content of 6' -O-caffeoyl arbutin in vaccinium camphorate tissue culture seedlings |
| CN112931223A (en) * | 2021-04-16 | 2021-06-11 | 山东大丰园农业有限公司 | Culture medium and culture method for blueberry tissue culture |
| US12010963B2 (en) | 2021-04-16 | 2024-06-18 | Shandong Dafengyuan Agriculture Co., Ltd. | Medium and culture method for blueberry tissue culture |
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