CN109971864A - A method of it is damaged using the expression quantity evaluation low dosage radiated by gamma-ray of zebrafish embryo brain ywhah gene - Google Patents
A method of it is damaged using the expression quantity evaluation low dosage radiated by gamma-ray of zebrafish embryo brain ywhah gene Download PDFInfo
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Abstract
The present invention relates to a kind of methods of expression quantity evaluation low dosage radiated by gamma-ray damage using zebrafish embryo brain ywhah gene.The dose-effect relationship between irradiation dose and ywhah gene expression amount received according to zebrafish embryo, by the inhibiting rate for calculating zebrafish embryo ywhah gene expression amount, it assesses low dosage radiated by gamma-ray to act on the comprehensive toxicity of zebrafish embryo, establishes biological damage evaluation method.This method have the advantages that it is easy to operate quickly, that high sensitivity, accuracy are good, environmental risk is small etc. is multiple.Long, complicated for operation, the specific problems such as not strong of period needed for solving existing low dosage radiated by gamma-ray method for estimating damage.
Description
Technical field
The invention belongs to low dosage radiated by gamma-ray Damage Evaluation technical fields, and in particular to a kind of to utilize zebra fish embryo
The method of the expression quantity evaluation low dosage radiated by gamma-ray damage of tire brain ywhah gene.
Background technique
Radiation treatment and irradiation diagnosis and nuclear power station, nuclear submarine, nuclear weapon, uranium mine staff in health care
Low dosage radiated by gamma-ray can all be received every year, the degree of the biological effect of low dosage radiated by gamma-ray induction generally compared with
It is low, the very low biological effect of this degree simultaneously also by body self-regeneration mechanism, homeostasis adjustment mechanism and other it is various in
The interference and influence of other factor, specific biological effect need the long period that could occur, this makes low dosage gamma ray spoke
Research and observation according to biological effect is very difficult, and let us is difficult scientific knowledge and correct assessment low dosage radiated by gamma-ray
Effect and consequence.Therefore, the evaluation method of research and development low dosage radiated by gamma-ray damage, lures for low dosage radiated by gamma-ray
The new treatment method of the diagnosis and exploitation for the human diseases led provides data support to prevent or treat irradiation damage, specifies low dose
Screening of the radiated by gamma-ray to the radiation damage effect and low dosage radiated by gamma-ray biomarker of biology is measured, is protection
One of the public being exposed under low dosage radiated by gamma-ray and the most pressing task of related practitioner health.
For model organism zebra fish (Danio rerio) because being easy to cultivate, the egg-laying season is short, and fertility is high, early development embryo
Tire is transparent and is widely used in the acute and chronic toxicity test of chemical mixture, drug screening and ecological toxicology test.Spot
Horse fish and the mankind have 87% homology, main signal path, physiological structure and function and mammal height on gene
It is similar, it means that the result of zebra fish toxicology test in most cases also can be applied to the mankind.US National is defended
Raw research institute advocates that zebra fish can be used as the biology of the third vertebrate animal model after mouse and rat, for studying
Human diseases after mouse and rat.In irradiation damage and radiation protection research field, zebra fish and its embryo also by
Screening and mechanism study for radiation protection drug.The invention patent penetrates gamma using the zebrafish embryo that inventor has found
The characteristic of line radiation sensitive, and the expression quantity of zebrafish embryo brain ywhah gene is deposited with low dosage radiated by gamma-ray dosage
In dose-effect relationship, low dosage radiated by gamma-ray is damaged using the expression quantity of zebrafish embryo brain ywhah gene
Carry out overall merit.
Summary of the invention
For above situation, the present invention provides a kind of expression quantity with zebrafish embryo brain ywhah gene to low dosage
Radiated by gamma-ray damages the method evaluated.Inventor is to the zebrafish embryo brain for receiving low dosage radiated by gamma-ray
Portion has carried out genome sequencing, it was found that the expression of zebrafish embryo brain ywhah gene is to low dosage radiated by gamma-ray
With sensibility, and being gradually increased with low dosage radiated by gamma-ray dosage, the expression quantity of ywhah gene gradually decrease,
The expression of bada just will increase, and so as to cause apoptosis rate rising, radiation injury degree increases.It is connect according to zebrafish embryo
Existing dose-effect relationship between the irradiation dose received and the expression quantity of brain ywhah gene, by detecting zebra fish embryo
The expression quantity of tire brain ywhah gene, to assess damage of the low dosage radiated by gamma-ray to zebrafish embryo.Its principle is,
On pi3k/Akt signal path, the radiated by gamma-ray of low dosage can induce the expression of ywhah gene to reduce, ywhah gene
Expression reduces the inhibiting effect reduction that will lead to bada gene, thus increase the expression of bada, and the expression of bada increases
It will lead to apoptosis rate rising, to damage increase.This method have it is easy to operate quickly, high sensitivity, accuracy it is good etc.
Multiple advantage.
It comprises the concrete steps that:
(1) acquisition of embryo；
(2) culture of embryo；
(3) radiated by gamma-ray；
(4) detection of ywhah gene expression amount；
(5) overall merit.
Its further step is:
The method of the acquisition of the embryo is:
Breeding oviposition is carried out using the zebra fish at 5 ~ 6 monthly ages.Zebra fish male and female are divided group rearing different by 2 d before mating
It raises in cylinder, breeds the eve of oviposition, male and female parent population is put into same mating device according to the ratio of 1:2, between female milter
It is separated with baffle.Control periodicity of illumination is 14 h of 14:10(illumination, and dark 10 h), will be between male and female parent population after 10 h of dark
Partition is taken away, and milter can chase raun, and the abdomen of body play raun, at this time milter sperm drainage, and raun starts to lay eggs.Deng production
After ovum, parent population is put back in respective raising cylinder, with suction pipe lightly by excrement in device of laying eggs and white dead
Ovum removes, and rinses the embryo in box in time with incubation fluid, and the methylene blue solution prevention fish of several drops 1 ‰ is added in incubation fluid
Ovum is infected by bacterial.Incubation fluid main formula is 10 L distilled water, 0.127 g KCl, 2.867 g NaCl, 0.817 g
MgSO4·7H2O, the anhydrous CaCl of 0.365 g2。
The method of the culture of the embryo is:
It after the zebrafish embryo of collection is rinsed well, is put into 12 orifice plate of tissue culture plate and cultivates, 5 pieces of every hole embryo, every plate is put
10 holes amount to 50 embryos.Embryo is moved using the suction pipe of 2 mL, it cannot be too big to the vibration of embryo when mobile.?
The incubation fluid that 10 mL are poured into 12 orifice plates, 28 DEG C ± 0.5 DEG C at a temperature of cultivate 2 h.
The method of the radiated by gamma-ray is:
To zebrafish embryo culture to 2 h, the zebrafish embryo of 2 hpf of after fertilization is collected, every group 100 pieces, is placed in 3 cm of diameter
Culture dish in, make zebrafish embryo under the radiated by gamma-ray of dosage rate 0.063-0.250 mGy/h and 28 DEG C ±
120 h are cultivated at a temperature of 0.5 DEG C.
The method of the detection of the ywhah gene expression amount is:
When Zebrafish Embryo is to 120 h, zebra fish head is cut under the microscope, extracts total serum IgE, carries out RT-PCR inspection
It surveys.The specific steps are that design primer ATGGGGGACCGGGAGCA, in RT-PCR instrument, setting program is (1) 95 DEG C 3
Min, (2) 95 DEG C of 10 s, (3) 67.3 DEG C of 30 s, (2) and (3) carry out 39 and recycle, after the completion of RT-PCR, finally carry out
Melting curve analysis determines the expression quantity of ywhah gene.
The method of the overall merit is:
In 120 h, the inhibiting rate of ywhah gene expression amount is calculated as follows:
(1)
Using the inhibiting rate for the ywhah gene expression amount being calculated, to low dosage radiated by gamma-ray to zebrafish embryo
Biological damage is assessed: when the inhibiting rate of zebrafish embryo brain ywhah gene expression amount is greater than 30% less than 60%, brain is thin
The change of born of the same parents' structure, nucleus crack, and the formation of apoptotic body, low dosage gamma ray can be observed in the agglutination of chromatin height
It irradiates and is to the biological damage grade of zebrafish embryoGrade；When the inhibiting rate of the ywhah gene expression amount of zebrafish embryo is greater than
60% and when less than 85%, nuclear membrane gap increases, and occurs vacuole structure, lysosome rupture, low dosage gamma ray spoke in cytoplasm
It is according to the biological damage grade to zebrafish embryoGrade；When the inhibiting rate of the ywhah gene expression amount of zebrafish embryo is greater than
When 85%, mitochondrial outer membrane dissolution, internal structure part disappears, and mitochondrial cristae is destructurized, low dosage radiated by gamma-ray pair
The biological damage grade of zebrafish embryo isGrade.Grade degree of injury is less thanGrade,Grade is less thanGrade.
When the impairment scale of low dosage radiated by gamma-ray reachesWhen grade, occupation work personnel should reinforce radiation protection；When
The impairment scale of low dosage radiated by gamma-ray reachesWhen grade, occupation work personnel should rest a period of time in time；Work as low dosage
The impairment scale of radiated by gamma-ray reachesWhen grade, occupation work personnel should be transferred from former work position, and receive appropriate treatment.
The present invention utilizes the expression of zebrafish embryo brain ywhah gene to the sensibility of low dosage radiated by gamma-ray,
The dose-effect relationship between irradiation dose and ywhah gene expression amount received according to zebrafish embryo, by calculating zebra
The inhibiting rate of fish embryo's ywhah gene expression amount, assessment low dosage radiated by gamma-ray make the comprehensive toxicity of zebrafish embryo
With establishing biological damage evaluation method.This method have it is easy to operate quickly, high sensitivity, accuracy are good, environmental risk is small etc.
Multiple advantage.Period needed for solving existing low dosage radiated by gamma-ray method for estimating damage is long, complicated for operation, specific not
The problems such as strong.
The a kind of of invention gives birth to low dosage radiated by gamma-ray with zebrafish embryo ywhah gene expression amount
The method of object Damage Evaluation has following technical advantage compared with art methods:
(1) it is convenient that the zebra fish selected by is drawn materials, cheap.
(2) short the time required to this method, the assessment of low dosage radiated by gamma-ray comprehensive toxicity effect can be obtained in 120 h
As a result.
(3) this method is easy to operate, high sensitivity, accuracy are good.
Specific embodiment
The invention will be further described below.
I material composition
The female milter of the zebra fish of adult, fishbowl, 12 orifice plates, incubation fluid, 1 ‰ methylene blue solution, microscope, gamma are penetrated
Line irradiation bomb.
II implementation method
Breeding oviposition is carried out using the zebra fish at 5 ~ 6 monthly ages.Zebra fish male and female are divided group rearing different by 2 d before mating
It raises in cylinder, breeds the eve of oviposition, male and female parent population is put into same mating device according to the ratio of 1:2, between female milter
It is separated with baffle.Control periodicity of illumination is 14 h of 14:10(illumination, and dark 10 h), will be between male and female parent population after 10 h of dark
Partition is taken away, and milter can chase raun, and the abdomen of body play raun, at this time milter sperm drainage, and raun starts to lay eggs.It collects
Zebra fish fertilized egg rinse well after, be put into 12 orifice plate of tissue culture plate and cultivate, 5 pieces of every hole fertilized eggs, every plate puts 10 holes
Amount to 50 embryos.The incubation fluid that 10 mL are poured into 12 orifice plates is cultivated under the conditions of 28 DEG C ± 0.5 DEG C of temperature.
To zebra fish fertilized egg culture to 2 h, the zebra fish fertilized egg of 2 hpf of after fertilization is collected, every group 100 pieces, is placed in 3 cm of diameter
Culture dish in, make zebra fish fertilized egg under the radiated by gamma-ray of dosage rate 0.063-0.250 mGy/h and at 28 DEG C
120 h are cultivated at a temperature of ± 0.5 DEG C.When Zebrafish Embryo is to 120 h, zebra fish head is cut under the microscope, is mentioned
Total serum IgE is taken, RT-PCR detection is carried out.The specific steps are that design primer ATGGGGGACCGGGAGCA, in RT-PCR instrument,
Setting program is (1) 95 DEG C of 3 min, (2) 95 DEG C of 10 s, (3) 67.3 DEG C of 30 s, and (2) and (3) carry out 39 circulations,
After the completion of RT-PCR, melting curve analysis is finally carried out to ywhah quantitative gene expression.
In 120 h, the inhibiting rate of zebrafish embryo brain ywhah gene expression amount is calculated by (1) formula.
According to the inhibiting rate for the zebrafish embryo brain ywhah gene expression amount being calculated, to low dosage gamma ray
The biological damage of zebrafish embryo is assessed in irradiation: when the inhibiting rate of zebrafish embryo brain ywhah gene expression amount is big
In 30% and when less than 60%, low dosage radiated by gamma-ray is to the biological damage grade of zebrafish embryoGrade；When zebra fish embryo
The ywhah gene expression amount of tire is greater than 60% when less than 85%, and low dosage radiated by gamma-ray damages the biology of zebrafish embryo
Hurting grade isGrade；When the ywhah gene expression amount of zebrafish embryo is greater than 85%, low dosage radiated by gamma-ray is to zebra fish
The biological damage grade of embryo isGrade.
When biological damage grade of the low dosage radiated by gamma-ray to zebrafish embryo reachesWhen grade, occupation work personnel
It should reinforce radiation protection；When biological damage grade of the low dosage radiated by gamma-ray to zebrafish embryo reachesWhen grade, professional work
Making personnel should the certain time of rest in time；When biological damage grade of the low dosage radiated by gamma-ray to zebrafish embryo reaches
When grade, occupation work personnel should be transferred from former work position, and receive appropriate treatment.
III embodiment
Embodiment 1
Breeding oviposition is carried out using the zebra fish at 6 monthly ages.Zebra fish male and female are divided group rearing in different raisings by 2 d before mating
In cylinder, the eve of oviposition is bred, male and female parent population is put into same mating device according to the ratio of 1:2, with gear between female milter
Plate separates.Control periodicity of illumination is 14 h of 14:10(illumination, and dark 10 h), after 10 h of dark, by the partition between male and female parent population
It takes away, milter can chase raun, and the abdomen of body play raun, at this time milter sperm drainage, and raun starts to lay eggs.The spot of collection
It after horse fish fertilized egg is rinsed well, is put into 12 orifice plate of tissue culture plate and cultivates, 5 pieces of every hole fertilized eggs, it is total that every plate puts 10 holes
50 embryos.The incubation fluid that 10 mL are poured into 12 orifice plates is cultivated under the conditions of 28 DEG C ± 0.5 DEG C of temperature.To spot
The culture of horse fish fertilized egg collects the zebra fish fertilized egg of 2 hpf of after fertilization, every group 100 pieces, is placed in the training of 3 cm of diameter to 2 h
It supports in ware, makes zebra fish fertilized egg under the radiated by gamma-ray of 0.063 mGy/h of dosage rate and at 28 DEG C ± 0.5 DEG C
At a temperature of cultivate 120 h, at this time zebrafish embryo receive radiated by gamma-ray irradiation dose be 7.5 mGy.At this point, aobvious
Zebra fish head is cut under micro mirror, extracts total serum IgE, carries out RT-PCR detection.The specific steps are that design primer is
ATGGGGGACCGGGAGCA, in RT-PCR instrument, setting program is (1) 95 DEG C of 3 min, (2) 95 DEG C of 10 s, (3) 67.3
DEG C 30 s, (2) and (3) carry out 39 circulations, after the completion of RT-PCR, finally carry out melting curve analysis, determine ywhah gene
Expression quantity.After the completion of measurement, the inhibiting rate of ywhah gene expression amount is calculated by (1) formula.When zebrafish embryo is 0.063
120 h are irradiated under the dosage rate of mGy/h, after the low dosage radiated by gamma-ray for receiving 7.5 mGy, ywhah gene expression amount
Inhibiting rate is 58.6%, and low dosage radiated by gamma-ray reaches the biological damage grade of zebrafish embryo at this timeGrade, professional work
Enhanced rad is answered to protect as personnel.
Embodiment 2
Breeding oviposition is carried out using the zebra fish at 6 monthly ages.Zebra fish male and female are divided group rearing in different raisings by 2 d before mating
In cylinder, the eve of oviposition is bred, male and female parent population is put into same mating device according to the ratio of 1:2, with gear between female milter
Plate separates.Control periodicity of illumination is 14 h of 14:10(illumination, and dark 10 h), after 10 h of dark, by the partition between male and female parent population
It takes away, milter can chase raun, and the abdomen of body play raun, at this time milter sperm drainage, and raun starts to lay eggs.The spot of collection
It after horse fish fertilized egg is rinsed well, is put into 12 orifice plate of tissue culture plate and cultivates, 5 pieces of every hole fertilized eggs, it is total that every plate puts 10 holes
50 embryos.The incubation fluid that 10 mL are poured into 12 orifice plates is cultivated under the conditions of 28 DEG C ± 0.5 DEG C of temperature.To spot
The culture of horse fish fertilized egg collects the zebra fish fertilized egg of 2 hpf of after fertilization, every group 100 pieces, is placed in the training of 3 cm of diameter to 2 h
It supports in ware, makes zebra fish fertilized egg under the radiated by gamma-ray of 0.125 mGy/h of dose rate and in 28 DEG C ± 0.5 DEG C of temperature
Degree is lower to cultivate 120 h, and the irradiation dose for the radiated by gamma-ray that zebrafish embryo receives at this time is 15 mGy.At this point, micro-
Zebra fish head is cut under mirror, extracts total serum IgE, carries out RT-PCR detection.The specific steps are that design primer is
ATGGGGGACCGGGAGCA, in RT-PCR instrument, setting program is (1) 95 DEG C of 3 min, (2) 95 DEG C of 10 s, (3) 67.3
DEG C 30 s, (2) and (3) carry out 39 circulations, after the completion of RT-PCR, finally carry out melting curve analysis to ywhah gene expression
It is quantitative.After the completion of measurement, the inhibiting rate of ywhah gene expression amount is calculated by (1) formula.When zebrafish embryo is in 0.125 mGy/h
Dosage rate under irradiate 120 h, after the low dosage radiated by gamma-ray for receiving 15 mGy, the inhibiting rate of ywhah gene expression amount
It is 80.7%, low dosage radiated by gamma-ray reaches the biological damage grade of zebrafish embryo at this timeGrade, at this point, occupation work
Personnel should the certain time of rest in time.
Embodiment 3
Breeding oviposition is carried out using the zebra fish at 6 monthly ages.Zebra fish male and female are divided group rearing in different raisings by 2 d before mating
In cylinder, the eve of oviposition is bred, male and female parent population is put into same mating device according to 1: 2 ratio, with gear between female milter
Plate separates.Control periodicity of illumination is 14: 10(illumination, 14 h, dark 10 h), after 10 h of dark, by between male and female parent population every
Plate is taken away, and milter can chase raun, and the abdomen of body play raun, at this time milter sperm drainage, and raun starts to lay eggs.It collects
It after zebra fish fertilized egg is rinsed well, is put into 12 orifice plate of tissue culture plate and cultivates, 5 pieces of every hole fertilized eggs, it is total that every plate puts 10 holes
Count 50 embryos.The incubation fluid that 10 mL are poured into 12 orifice plates is cultivated under the conditions of 28 DEG C ± 0.5 DEG C of temperature.To
The culture of zebra fish fertilized egg collects the zebra fish fertilized egg of 2 hpf of after fertilization, every group 100 pieces, is placed in 3 cm's of diameter to 2 h
In culture dish, make zebra fish fertilized egg under the radiated by gamma-ray of 0.250 mGy/h of dosage rate and at 28 DEG C ± 0.5 DEG C
At a temperature of cultivate 120 h, at this time zebrafish embryo receive radiated by gamma-ray irradiation dose be 30 mGy.At this point,
Zebra fish head is cut under microscope, extracts total serum IgE, carries out RT-PCR detection.The specific steps are that design primer is
ATGGGGGACCGGGAGCA, in RT-PCR instrument, setting program is (1) 95 DEG C of 3 min, (2) 95 DEG C of 10 s, (3) 67.3
DEG C 30 s, (2) and (3) carry out 39 circulations, after the completion of RT-PCR, finally carry out melting curve analysis to ywhah gene expression
It is quantitative.After the completion of measurement, the inhibiting rate of ywhah gene expression amount is calculated by (1) formula.When zebrafish embryo is in 0.250 mGy/h
Dosage rate under irradiate 120 h, after the low dosage radiated by gamma-ray for receiving 30 mGy, the inhibiting rate of ywhah gene expression amount
It is 92.5%, low dosage radiated by gamma-ray reaches the biological damage grade of zebrafish embryo at this timeGrade, at this point, professional work
Former work position should be transferred from by making personnel, and suitably be treated.
It is above only better embodiment of the invention, according to the above-mentioned design, those skilled in the art
Can also to this various modification can be adapted and transformation.For example, the type of transformation irradiated rays, the type for converting zebra fish, zebra fish embryo
The incubation time of tire adjusts radiation dose rate, using different irradiation times and irradiation dose, transformation comprehensive toxicity appraisement system etc.
Deng.However, similar this transformation and modification belongs to essence of the invention.
Claims (3)
1. a kind of side of the expression quantity evaluation low dosage radiated by gamma-ray damage using zebrafish embryo brain ywhah gene
Method is reduced according to the expression quantity with the increase for receiving radiated by gamma-ray dosage, zebrafish embryo brain ywhah gene, from
And the expression of bada gene increases, and apoptosis rate is caused to rise, radiation injury degree increases, which is characterized in that passes through calculating
The inhibiting rate of zebrafish embryo ywhah gene expression amount, comprehensive poison of the assessment low dosage radiated by gamma-ray to zebrafish embryo
Property effect, establish biological damage evaluation method,
It comprises the concrete steps that:
(1) acquisition of embryo；
(2) culture of embryo；
(3) radiated by gamma-ray；
(4) detection of ywhah gene expression amount；
(5) overall merit；
The method of the acquisition of the embryo is:
Breeding oviposition is carried out using the zebra fish at 5 ~ 6 monthly ages, and rinses embryo with incubation fluid；
The method of the culture of the embryo is:
It after the zebrafish embryo of collection is rinsed well, is put into tissue culture plate, the incubation fluid of 10 mL is poured into culture plate,
2 h are cultivated at a temperature of 28 DEG C ± 0.5 DEG C；
The method of the radiated by gamma-ray is:
To zebrafish embryo culture to 2 h, make zebrafish embryo in the gamma ray spoke of dosage rate 0.063-0.250 mGy/h
According to it is lower and 28 DEG C ± 0.5 DEG C at a temperature of cultivate 120 h；
The method of the detection of the ywhah gene expression amount is:
When Zebrafish Embryo is to 120 h, zebra fish head is cut under the microscope, extracts total serum IgE, carries out RT-PCR inspection
It surveys, the specific steps are that, design primer ATGGGGGACCGGGAGCA, in RT-PCR instrument, setting program is (a) 95 DEG C 3
Min, (b) 95 DEG C of 10 s, (c) 67.3 DEG C of 30 s (b) carry out 39 and recycle, after the completion of RT-PCR, finally carry out with (c)
Melting curve analysis determines the expression quantity of ywhah gene；
The method of the overall merit is:
In 120 h, the inhibiting rate of ywhah gene expression amount is calculated as follows:
(1)
Using the inhibiting rate for the ywhah gene expression amount being calculated, to low dosage radiated by gamma-ray to zebrafish embryo
Biological damage is assessed.
2. a kind of expression quantity using zebrafish embryo brain ywhah gene according to claim 1 evaluates low dosage gal
The method of horse x ray irradiation x damage, which is characterized in that the hatching formula of liquid is 10 L distilled water, 0.127 g KCl, 2.867
g NaCl、0.817 g MgSO4·7H2O, the anhydrous CaCl of 0.365 g2。
3. a kind of expression quantity using zebrafish embryo brain ywhah gene according to claim 1 evaluates low dosage gal
The method of horse x ray irradiation x damage, which is characterized in that the assessment particular content are as follows: when zebrafish embryo brain ywhah gene
When the inhibiting rate of expression quantity is greater than 30% less than 60%, low dosage radiated by gamma-ray is to the biological damage grade of zebrafish embryoGrade；When the inhibiting rate of the ywhah gene expression amount of zebrafish embryo be greater than 60% and when less than 85%, low dosage gamma ray spoke
It is according to the biological damage grade to zebrafish embryoGrade；When the inhibiting rate of the ywhah gene expression amount of zebrafish embryo is greater than
When 85%, low dosage radiated by gamma-ray is to the biological damage grade of zebrafish embryoGrade,Grade degree of injury is less thanGrade,
Grade is less thanGrade.
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| CN113035356A (en) * | 2021-03-03 | 2021-06-25 | 首都医科大学 | Evaluation method for damage of cardiovascular and nervous systems by atmospheric particulates |
| EP3854453A1 (en) * | 2020-01-24 | 2021-07-28 | CHUV, Centre hospitalier universitaire vaudois | Flash detector |
| CN117265080A (en) * | 2023-09-19 | 2023-12-22 | 山东省科学院生物研究所 | Zebra fish gene markers and application thereof in assessing risk of nerve injury caused by environmental pollutants |
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| EP3854453A1 (en) * | 2020-01-24 | 2021-07-28 | CHUV, Centre hospitalier universitaire vaudois | Flash detector |
| CN113035356A (en) * | 2021-03-03 | 2021-06-25 | 首都医科大学 | Evaluation method for damage of cardiovascular and nervous systems by atmospheric particulates |
| CN113035356B (en) * | 2021-03-03 | 2023-12-08 | 首都医科大学 | Method for evaluating damage of atmospheric particulates to cardiovascular and nervous system |
| CN117265080A (en) * | 2023-09-19 | 2023-12-22 | 山东省科学院生物研究所 | Zebra fish gene markers and application thereof in assessing risk of nerve injury caused by environmental pollutants |
| CN117265080B (en) * | 2023-09-19 | 2024-02-23 | 山东省科学院生物研究所 | Zebra fish gene markers and application thereof in assessing risk of nerve injury caused by environmental pollutants |
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