CN109971811A - A kind of enzymatic isolation method prepares the method for corn functional peptides and the corn functional peptides of preparation - Google Patents
A kind of enzymatic isolation method prepares the method for corn functional peptides and the corn functional peptides of preparation Download PDFInfo
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Abstract
The method of corn functional peptides and the corn functional peptides of preparation are prepared the present invention provides a kind of enzymatic isolation method.It is dissolved method includes the following steps: maize yellow-powder is sieved, adjusts pH to 8~12, and stirring in water bath obtains supernatant in enzymatic vessel, and supernatant is heated, adjust pH to 6~9, stirring centrifugation obtains corn separation protein；Enzyme preparation is added to be digested, obtains enzymolysis liquid, by enzymolysis liquid enzyme deactivation, sterilization treatment, corn functional peptides powder is made after spray drying.The present invention prepares corn functional peptides using enzymatic isolation method, realizes low value resource higher value application, preparation method simple production process, corn functional peptides purity is high obtained, content of peptides height.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of method that enzymatic isolation method prepares corn functional peptides.
Background technique
China is maize production big country, and corn yield occupies second place of the world, there is zein resource abundant, maize
Powder, one of the by-product as production of corn starch, includes about 60% protein, by zeins, glutelin, ball
Albumen and albumin composition.Although maize yellow-powder is a kind of resourceful, low-cost natural plant protein raw material, but due to
Wherein the content of lysine and tryptophan is not high, and the amino acid of contained protein forms imbalance, is a kind of typical non-Quan Ying
Support albumen.In addition, protein contained by maize yellow-powder is insoluble in water, composition is again more complicated, and mouthfeel is also very coarse, processability
Can be bad, thus it is lower as conventional protein nutrient biological value, either in field of food still in feedstuff industry
All few people make inquiries, and seriously limit application of the maize yellow-powder in food and medicine industry, or even have many places will also
Be not added together with other by-products recycling just discharge naturally in vain, this is not only a kind of very big wave to grain resource
Take, while a degree of pollution also is caused to ecological environment.In fact, with the development of modern science and technology, utilizing maize yellow-powder
Extractable natural food colour, zeins etc., since albumen Glutamic Acid and branched-amino acid content are higher, can make
The standby corn functional peptides with different physiological roles, such as glutamine peptide, high F value oligopeptide, blood pressure lowering peptide and corn protein peptide
Deng the added value of corn can be greatly improved.If can carry out modifying to zein and be applied to food industry, market price
Value will substantially increase, therefore exploitation maize yellow-powder becomes a current weight with the comprehensive utilization ratio for further increasing corn
Want research topic.
Proteolysis refers to the process of protein in various albumen enzyme effects decline solution at amino acid, enzymatic hydrolysis often one kind
Do not weaken food protein nutritive value, and obtains the short-cut method of more preferable food protein functional characteristic.Pass through enzyme process water
Solution improves the processing performance of protein food, trophism and to release physiological hygiene function in widespread attention.In recent years,
The country is also gradually active to the research of corn peptide, reports gradually more.But there are still many problems, to sum up having following several
Point: first is that increasing the cost of downstream separation purifying, the corn peptide product prepared directly with maize yellow-powder to digest raw material mostly
Matter is not high, and such as patent of invention CN201110004804.5, disclosing " a kind of preparation method of corn peptide " is exactly to select maize
Powder is added ethyl alcohol and is directly digested；Second is that mostly use external good protease greatly to digest, such as patent of invention
CN200310115958.7 discloses " corn peptide, preparation method and applications ", is exactly using external high-quality alkali protease system
Standby corn Gly-His-Lys, this kind of method selling at exorbitant prices increase the cost of peptide production.
Summary of the invention
The method of corn functional peptides and the corn functional peptides of preparation are prepared the purpose of the present invention is to provide a kind of enzymatic isolation method,
This method simple process, and corn functional peptides obtained activity is high, free from extraneous odour.
The preparation method of corn functional peptides of the invention, includes the following steps:
A, maize yellow-powder is sieved and is dissolved, adjust pH to 8~12, centrifugation is stirred in enzymatic vessel, takes supernatant；
B, above-mentioned supernatant is heated, adjusts pH to 6~9, stirring centrifugation is separated by solid-liquid separation, obtains corn separation protein；
C, the corn separation protein is 45~70 DEG C in enzyme preparation and temperature, and enzymolysis time carries out enzyme under conditions of being 2~8h
Solution, obtains enzymolysis liquid；
D, it is 90~125 DEG C in temperature by the enzymolysis liquid, under conditions of the time is 0.5~3h after progress destroy the enzyme treatment, obtains
Enzymolysis solution after enzyme deactivation；
E, the enzymolysis solution after enzyme deactivation is spray-dried after concentration, emulsifying, sterilizing and obtains corn functional peptides powder.
Preparation method according to the present invention, it is preferable that in step A, maize yellow-powder crosses 40~80 meshes.
Preparation method according to the present invention, it is preferable that in step A, pH value is adjusted to 8~12 using highly basic；The highly basic
Selected from one of piece alkali, liquid alkaline, ammonium hydroxide or a variety of.
Preparation method according to the present invention, it is preferable that in step A, dissolution select distilled water, mixing time be 40~
60min。
Preparation method according to the present invention, it is preferable that in step B, heating of supernatant is to 70~100 DEG C.
Preparation method according to the present invention, it is preferable that in step B, using strong acid for adjusting pH value to 6~9；The strong acid choosing
From one of hydrochloric acid, sulfuric acid, phosphoric acid or a variety of.
Preparation method according to the present invention, it is preferable that in step C, the alkali protease, medium temperature amylase, neutral egg
White enzyme, flavor protease, trypsase enzyme activity can be 1000~5000U/g, enzyme preparation additional amount can be described
0.2~3wt% of corn separation protein weight.
Preparation method according to the present invention, it is preferable that in step C, the enzyme preparation selects medium temperature amylase, neutral protein
The complex enzyme formulation of enzyme, alkali protease is 1:1:2 composition according to weight ratio.
Preparation method according to the present invention, it is preferable that in step E, the enzymolysis liquid after the destroy the enzyme treatment is concentrated, homogeneous
Emulsification, is spray-dried after sterilizing, obtains corn functional peptides powder.
Preparation method according to the present invention, it is preferable that in step E, at 60 DEG C or more, spray drying exists the sterilising temp
It is carried out at 80~100 DEG C.
It is according to the present invention another kind preparation method, it is preferable that in step E, by the enzymolysis liquid after the destroy the enzyme treatment into
Row centrifugation, centrifugate is filtered through plate and frame filter press first, then through ceramic membrane filter, obtains active peptide permeate；By the activity
The concentration of peptide filtrate, spray drying, to obtain corn functional peptides powder；Contain combination filtration adjuvant, institute in the plate and frame filter press
Combination filtration adjuvant is stated to be made of active carbon, perlite and diatomite according to weight ratio 6~7: 1~2: 2~3.
The present invention also provides a kind of corn functional peptides, which is prepared using the above method.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
The present invention is further detailed explanation below.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below, but protection scope of the present invention is not limited to
This.
In the present invention, the maize yellow-powder is the by-product that corn wet produces starch.
The preparation method of corn functional peptides of the invention, includes the following steps:
A, maize yellow-powder is sieved and is dissolved, adjust pH to 8~12, centrifugation is stirred in enzymatic vessel, takes supernatant；
B, above-mentioned supernatant is heated, adjusts pH to 6~9, stirring centrifugation obtains corn separation protein；
C, the corn separation protein is 45~70 DEG C in enzyme preparation and temperature, and enzymolysis time carries out enzyme under conditions of being 2~8h
Solution, obtains enzymolysis liquid；
D, it is 90~125 DEG C in temperature by the enzymolysis liquid, under conditions of the time is 0.5~3h after progress destroy the enzyme treatment, obtains
Enzymolysis solution after enzyme deactivation；
E, the enzymolysis solution after enzyme deactivation is spray-dried after concentration, emulsifying, sterilizing and obtains corn functional peptides powder.
Preparation method according to the present invention, by the by-product after wet production starch --- maize yellow-powder crosses 40 in step A
~80 meshes, preferably 55~70 meshes, then plus distilled water dissolves, using the maize yellow-powder of partial size of the invention, Neng Gou
Enzymatic hydrolyzation is effectively improved in the enzymolysis step in later period, makes full use of the protein ingredient in maize yellow-powder.Described size mixing preferably exists
Stirring is lower to be carried out, so that the ingredient in maize yellow-powder be made to be fully dissolved out.In step A, amount of water is the 8~15 of maize yellow-powder weight
Times, preferably 10~14 times.Preferably, after the completion of sizing mixing process, progress mechanical stirring 40~60 minutes, preferably 42~58
Minute, to keep liquid uniform, convenient for centrifugation.
In step A, pH value is adjusted to 8~12, preferably 10.8~12 by the way that highly basic is added；The highly basic can be piece
One of alkali, liquid alkaline, ammonium hydroxide are a variety of, preferably piece alkali；The solid-liquid separating method can be filtering, centrifugation etc., and excellent
It is selected as being centrifugated.
Supernatant in step B, is heated to 70~100 DEG C, preferably 85 using collet by preparation method according to the present invention
~95 DEG C；Using strong acid by the supernatant adjust pH to 6~9, preferably 6.5~8.1, the strong acid can selected from hydrochloric acid,
One of nitric acid, sulfuric acid are a variety of, preferably hydrochloric acid, protein precipitation can be made to come out, and reduce to proteinaceous nutrient substance
Destruction, and finally obtained corn functional peptides Product Activity is more preferable, while pH is adjusted to above-mentioned alkalescent, is more advantageous to this
The enzyme preparation of invention is digested.
Preparation method according to the present invention, in step C, enzyme preparation is selected from alkali protease, neutral proteinase, medium temperature starch
One of enzyme, flavor protease, trypsase are a variety of, preferably medium temperature amylase, neutral proteinase and alkali protease
It is digested according to weight ratio 1:1:2 composition, enzymolysis process of the invention is added at one time above-mentioned complex enzyme formulation, and above-mentioned
It is digested under the conditions of pH, more preferably hydrolysis result can be obtained.Enzyme preparation of the invention for the protein in maize yellow-powder at
Point enzymolysis efficiency it is high, hence it is evident that be higher than other enzyme preparations, preparation method according to the present invention, the additional amount of the enzyme preparation can be with
For 0.2~3wt% of the corn separation protein weight, preferably 0.9~3wt%, more preferably 1.8~2.8wt%.This hair
In bright, alkali protease, medium temperature amylase, neutral proteinase enzyme activity can be 1000~5000U/g, hydrolysis temperature is
45~70 DEG C, enzymolysis time is 2~8h；Preferably, selecting enzyme activity is 2000~4000U/g, and hydrolysis temperature is 48~62 DEG C,
Enzymolysis time is 2~6h.The additional amount of above-mentioned enzyme preparation, enzymolysis time and at a temperature of, zein can realize effective enzymatic hydrolysis.
Preparation method according to the present invention, in step D, the temperature of the destroy the enzyme treatment is 90~125 DEG C, the time 0.5
~3h；Preferably, enzyme-removal temperature is 113~125 DEG C, and the time is 1~2.7h.
According to embodiment of the present invention, in step E, the enzymolysis liquid after the destroy the enzyme treatment is concentrated, homogenized milk
Change, after sterilizing, spray drying obtains corn functional peptides powder, which is full nutrient corn active peptide.The full nutrition
In corn functional peptides product, peptide content >=35wt%；Wherein it is preferred to the spray-drying operation is carried out at 80~100 DEG C,
To effectively keep the activity of corn functional peptides.
Enzymolysis liquid after the destroy the enzyme treatment is centrifuged, centrifugate by another preferred embodiment according to the present invention
It is filtered first through plate and frame filter press, then through ceramic membrane filter, obtains active peptide permeate；The active peptide filtrate is concentrated, is gone out
It is spray-dried after bacterium, to obtain corn functional peptides powder, which is purification corn functional peptides powder.
In the present invention, containing combination filtration adjuvant in the plate and frame filter press, the combination filtration adjuvant is by active carbon, treasure
Zhu Yan and diatomite composition, and the weight ratio of active carbon, perlite and diatomite is 6~7: 1~2: 2~3.It is according to the present invention
One preferred embodiment, the weight ratio for combining active carbon, perlite and diatomite in filtration adjuvant are 7: 1.5: 2.
Inventors have found that the corn functional peptides of existing method preparation have peculiar smell, and there is bitter taste, poor taste；A variety of filtration adjuvant lists
With being difficult to simultaneously remove the two.Filtration adjuvant of the invention can effectively remove corn per se with peculiar smell and bitter taste,
Simplify process flow.In the present invention, the aperture of the ceramic membrane can be 20~500nm, more preferably 20~300mm.It adopts
With such ceramic membrane aperture, the further purification of enzymolysis liquid can be realized.
After filtration treatment of the invention, peptide content >=90wt% in obtained purification corn functional peptides powder.
In general, the spray-drying operation of peptide is carried out at 120~140 DEG C, it is considered that such drying temperature is for peptide
Activity influence is little.However, it is found by the inventors that the temperature of existing spray drying significantly affects the activity of peptide.And in the present invention,
Preferably, the spray-drying operation is carried out at 80~100 DEG C, to effectively keep the activity of corn functional peptides.
The embodiment of the present invention is described in detail below, but protection scope of the present invention is not limited.
Embodiment 1
Present embodiments provide a kind of method for preparing full nutrient corn active peptide using enzymatic isolation method, comprising the following steps:
A, maize yellow-powder is crossed into 60 meshes, the water mixed dissolution of 11 times of amounts is added, adjust pH to 11.2 with sodium hydroxide solution,
It is stirred 50 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 92.1 DEG C, adjusts pH to 7, precipitating proteins, mechanical stirring, centrifugation point with hydrochloric acid solution
From insoluble slag is taken, it is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 3000U/g；The dosage of enzyme preparation is the 2.1wt% of solid content weight in suspension；Hydrolysis temperature is
54.4 DEG C, enzymolysis time 3h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 121 DEG C, time 1.5h；
E, enzymolysis solution after enzyme deactivation is concentrated, emulsifying, after sterilizing at 121 DEG C, in 85 DEG C in middle low-temperature spray drying tower
Spray drying, obtains full nutrient corn active peptide powder.
Embodiment 2
Present embodiments provide a kind of method for preparing full nutrient corn active peptide using enzymatic isolation method, comprising the following steps:
A, maize dried bean noodles is crossed into 40 meshes, the water mixed dissolution of 8 times of amounts is added, adjust pH to 8.2 with sodium hydroxide solution,
It is stirred 41 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 72 DEG C, adjusts pH to 6.5, precipitating proteins, mechanical stirring, centrifugation point with hydrochloric acid solution
From insoluble slag is taken, it is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 1500U/g；The dosage of enzyme preparation is the 0.8wt% of solid content weight in suspension；Hydrolysis temperature is
45 DEG C, enzymolysis time 2h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 90 DEG C, time 0.5h；
E, enzymolysis solution after enzyme deactivation is concentrated, emulsifying, it is spraying in 81 DEG C in middle low-temperature spray drying tower after 121 DEG C of sterilizings
It is dry, obtain full nutrient corn active peptide powder.
Embodiment 3
Present embodiments provide a kind of method for preparing full nutrient corn active peptide using enzymatic isolation method, comprising the following steps:
A, maize yellow-powder to be crossed into 80 meshes, the water of 14.5 times of amounts is added, mixed dissolution is adjusted to pH12 with sodium hydroxide solution,
It is stirred 60 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 100 DEG C, adjusts pH to 8.9, precipitating proteins, mechanical stirring, centrifugation with hydrochloric acid solution
Separation takes insoluble slag, is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 4500U/g；The dosage of enzyme preparation is the 3.0wt% of solid content weight in suspension；Hydrolysis temperature is
68.5 DEG C, enzymolysis time 5.5h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 125 DEG C, time 3h；
E, enzymolysis solution after enzyme deactivation is concentrated, emulsifying, is sprayed in middle low-temperature spray drying tower in 100 DEG C after 121 DEG C of sterilizings
Mist is dry, obtains full nutrient corn active peptide powder.
Embodiment 4
Present embodiments provide a kind of method for preparing purification corn functional peptides using enzymatic isolation method, comprising the following steps:
A, maize yellow-powder to be crossed into 60 meshes, the water of 11 times of amounts is added, mixed dissolution is adjusted to pH11.2 with sodium hydroxide solution,
It is stirred 50 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 92.1 DEG C, adjusts pH to 7, precipitating proteins, mechanical stirring, centrifugation point with hydrochloric acid solution
From insoluble slag is taken, it is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 3000U/g；The dosage of enzyme preparation is the 2.1wt% of solid content weight in suspension；Hydrolysis temperature is
54.4 DEG C, enzymolysis time 3h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 121 DEG C, time 1.5h；
E, enzymolysis solution after enzyme deactivation is centrifuged, centrifugate is filtered through plate and frame filter press, and filtration adjuvant, mistake are contained in plate and frame filter press
Filter auxiliary agent is made of active carbon, perlite and diatomite according to weight ratio 7: 1.5: 2；Via hole diameter is the ceramic membrane mistake of 400nm again
Filter, obtains active peptide permeate；The active peptide permeate is concentrated, in 85 in middle low-temperature spray drying tower after 121 DEG C of sterilizings
DEG C spray drying, obtain purification corn functional peptides powder.
Embodiment 5
Present embodiments provide a kind of method for preparing purification corn functional peptides using enzymatic isolation method, comprising the following steps:
A, maize yellow-powder to be crossed into 40 meshes, the water of 8 times of amounts is added, mixed dissolution is adjusted to pH8.2 with sodium hydroxide solution,
It is stirred 41 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 72 DEG C, adjusts pH to 6.5, precipitating proteins, mechanical stirring, centrifugation point with hydrochloric acid solution
From insoluble slag is taken, it is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 1500U/g；The dosage of enzyme preparation is the 0.8wt% of solid content weight in suspension；Hydrolysis temperature is
45 DEG C, enzymolysis time 2h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 90 DEG C, time 0.5h；
E, enzymolysis solution after enzyme deactivation is centrifuged, centrifugate is filtered through plate and frame filter press, and filtration adjuvant, mistake are contained in plate and frame filter press
Filter auxiliary agent is made of active carbon, perlite and diatomite according to weight ratio 6.7: 1.8: 2；Via hole diameter is the ceramic membrane of 400nm again
Filtering, obtains active peptide permeate；By the active peptide permeate be concentrated, 121 DEG C sterilizing after in middle low-temperature spray drying tower in
81 DEG C of spray drying obtain purification corn functional peptides powder.
Embodiment 6
Present embodiments provide a kind of method for preparing purification corn functional peptides using enzymatic isolation method, comprising the following steps:
A, maize yellow-powder to be crossed into 80 meshes, the water of 14.5 times of amounts is added, mixed dissolution is adjusted to pH12 with sodium hydroxide solution,
It is stirred 60 minutes in enzymatic vessel, is centrifuged, takes supernatant；
B, the supernatant is heated to 100 DEG C, adjusts pH to 8.9, precipitating proteins, mechanical stirring, centrifugation with hydrochloric acid solution
Separation takes insoluble slag, is dried to powder and obtains corn separation protein；
C, add water to size mixing corn separation protein, enzyme preparation is added and is digested, enzymolysis liquid is obtained；The enzyme preparation is medium temperature starch
Enzyme, neutral proteinase, alkali protease are formed according to weight ratio 1:1:2 and are added, medium temperature amylase, neutral proteinase, alkaline egg
The enzyme activity of white enzyme is 4500U/g；The dosage of enzyme preparation is the 3.0wt% of solid content weight in suspension；Hydrolysis temperature is
68.5 DEG C, enzymolysis time 5.5h；
D, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 125 DEG C, time 3h；
E, enzymolysis solution after enzyme deactivation is centrifuged, centrifugate is filtered through plate and frame filter press, and filtration adjuvant, mistake are contained in plate and frame filter press
Filter auxiliary agent is made of active carbon, perlite and diatomite according to weight ratio 6.5: 2: 3；Via hole diameter is the ceramic membrane mistake of 400nm again
Filter, obtains active peptide permeate；The active peptide permeate is concentrated, in 100 in middle low-temperature spray drying tower after 121 DEG C of sterilizings
DEG C spray drying, obtain purification corn functional peptides powder.
Experimental example
Peptide content in corn functional peptides in Examples 1 to 6 is determined, the results are shown in Table 1.It is living to corn in Examples 1 to 6
Taste is judged in property peptide, the results are shown in Table 2.
The wherein content assaying method of peptide are as follows: carried out according to the measuring method of peptide content in 22492 soy peptide powder of GB/T.
Bitter taste estimate method are as follows: take 1g corn functional peptides to be added in 10ml warm water and stir evenly, extension rate is calculated as hardship
Taste value calculates the average bitterness value of 20 people.
Fishy smell evaluation method are as follows: evaluation method are as follows: 0- is without fishy smell；1- slightly has fishy smell；2- fishy smell is weaker；3- has fishy smell；4-
Fishy smell is general；5- fishy smell is laid particular stress on；6- fishy smell is heavier；7- fishy smell is very heavy, calculates the average fishy smell value of 20 people.
Table 1
Table 2
Present invention is not limited to the embodiments described above, without departing from the essence of the present invention, those skilled in the art
Member it is contemplated that any deformation, improvement, replacement each fall within the scope of the present invention.
Claims (10)
1. a kind of method that enzymatic isolation method prepares corn functional peptides, which comprises the steps of:
A, maize yellow-powder is sieved and is dissolved, adjust pH to 8~12, centrifugation is stirred in enzymatic vessel, takes supernatant；
B, above-mentioned supernatant is heated, adjusts pH to 6~9, stirring centrifugation is separated by solid-liquid separation, obtains corn separation protein；
C, the corn separation protein is 45~70 DEG C in enzyme preparation and temperature, and enzymolysis time carries out enzyme under conditions of being 2~8h
Solution, obtains enzymolysis liquid；
D, it is 90~125 DEG C in temperature by the enzymolysis liquid, under conditions of the time is 0.5~3h after progress destroy the enzyme treatment, obtains
Enzymolysis solution after enzyme deactivation；
E, the enzymolysis solution after enzyme deactivation is spray-dried after concentration, emulsifying, sterilizing and obtains corn functional peptides powder.
2. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that beautiful in the step A
Cream-coloured powder crosses 40~80 meshes.
3. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that in the step A, adjust
It saves pH and selects one of piece alkali, liquid alkaline, ammonium hydroxide or a variety of.
4. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that in the step B, add
Hot supernatant is to 70~100 DEG C.
5. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that in the step B, adjust
It saves pH value and selects one of hydrochloric acid, sulfuric acid, phosphoric acid or a variety of.
6. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that in the step C, enzyme
Preparation is selected from one of alkali protease, medium temperature amylase, neutral proteinase, flavor protease, trypsase or a variety of.
7. the method that enzymatic isolation method according to claim 6 prepares corn functional peptides, which is characterized in that in the step C, institute
State alkali protease, medium temperature amylase, neutral proteinase, flavor protease, trypsase enzyme activity can for 1000~
5000U/g, enzyme preparation additional amount can be 0.2~3wt% of the corn separation protein weight.
8. the method that enzymatic isolation method according to claim 1 prepares corn functional peptides, which is characterized in that in the step E, institute
Sterilising temp is stated at 60 DEG C or more, spray drying carries out at 80~100 DEG C.
9. the method that -8 any enzymatic isolation methods prepare corn functional peptides according to claim 1, which is characterized in that the step
In A, distilled water is selected in dissolution, and mixing time is 40~60 minutes.
10. a kind of corn functional peptides, which is characterized in that the corn functional peptides are using described in any one according to claim 1~9
Method be prepared.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111549086A (en) * | 2020-05-19 | 2020-08-18 | 禹城保立康生物饲料有限公司 | Preparation method for preparing corn peptide by heat treatment-assisted enzyme method |
| CN115960167A (en) * | 2022-08-18 | 2023-04-14 | 齐齐哈尔大学 | Corn anti-adhesion peptide and preparation method and application thereof |
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2019
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111549086A (en) * | 2020-05-19 | 2020-08-18 | 禹城保立康生物饲料有限公司 | Preparation method for preparing corn peptide by heat treatment-assisted enzyme method |
| CN115960167A (en) * | 2022-08-18 | 2023-04-14 | 齐齐哈尔大学 | Corn anti-adhesion peptide and preparation method and application thereof |
| CN115960167B (en) * | 2022-08-18 | 2023-11-07 | 齐齐哈尔大学 | Corn anti-adhesion peptide and preparation method and application thereof |
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Application publication date: 20190705 |