CN109971366A - The accurate injection device of acid protease in a kind of gelatin acid hemolysis process - Google Patents

The accurate injection device of acid protease in a kind of gelatin acid hemolysis process Download PDF

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Publication number
CN109971366A
CN109971366A CN201910297526.3A CN201910297526A CN109971366A CN 109971366 A CN109971366 A CN 109971366A CN 201910297526 A CN201910297526 A CN 201910297526A CN 109971366 A CN109971366 A CN 109971366A
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CN
China
Prior art keywords
protease
acid
gelatin
control device
hemolysis process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910297526.3A
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Chinese (zh)
Inventor
刘侨
王帅
曹和兴
张岩峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Huayu Biotechnology Co Ltd
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Sichuan Huayu Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Huayu Biotechnology Co Ltd filed Critical Sichuan Huayu Biotechnology Co Ltd
Priority to CN201910297526.3A priority Critical patent/CN109971366A/en
Publication of CN109971366A publication Critical patent/CN109971366A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09HPREPARATION OF GLUE OR GELATINE
    • C09H3/00Isolation of glue or gelatine from raw materials, e.g. by extracting, by heating
    • C09H3/02Purification of solutions of gelatine

Abstract

The invention discloses the accurate injection devices of acid protease in a kind of gelatin acid hemolysis process, including driving device, with the feeding device driven by driving device, feeding device one end is connect with protease container, feeding device also pipeline connects fill assembly, driving device signal connects control device, and protease container includes the detection device for detecting protease state, and detection device is connect with control device signal.The accurate injection device of acid protease can in real time be monitored protease local environment in a kind of gelatin acid hemolysis process of the invention, guarantee that protease is in optimum Working;The accurate injection device of acid protease in real time regulates and controls protease local environment in gelatin acid hemolysis process, and intelligence degree is high;The accurate injection device structure of acid protease is simple, functional good in gelatin acid hemolysis process, has good economy and practicability.

Description

The accurate injection device of acid protease in a kind of gelatin acid hemolysis process
Technical field
The present invention relates to manufacturing of gelatin fields, precisely inject more particularly to acid protease in a kind of gelatin acid hemolysis process Device and method.
Background technique
Gelatin be by the connective tissues such as animal skin, bone, sarolemma, flesh evil spirit collagenous portion degradation and become white or Faint yellow, translucent, micro-strip gloss thin slice or powder;Therefore it is called and does animal gelatin, fat glue;Industrial gelatine is colourless to yellowish The transparent or semitransparent equal thin slices of color or powder;It is tasteless, it is odorless;The water swelling in cold water;It is dissolved in hot water;It is dissolved in sweet oil and vinegar Acid does not dissolve in ethyl alcohol and ether.
Include acid hemolysis process in the production process of gelatin, needs to carry out assisted reaction using acid protease, it is bright to improve The acidolysis rate of glue generally requires accurately adding set in acid hemolysis process, is equably added to gelatin acid hydrolysis tank to reach In, protease is because of stringent living environment, it is easy to be denaturalized, so that the reaction efficiency of final gelatin is too low.
Summary of the invention
In order to solve the above technical problems, the present invention, which provides acid protease in a kind of gelatin acid hemolysis process, precisely injects dress Set, can real-time detection protease state, and give protease and the living environment of one signal be provided, while intelligence degree is high Device, the technical solution adopted is as follows:
The accurate injection device of acid protease in a kind of gelatin acid hemolysis process, including driving device, and by driving device driving Feeding device, feeding device one end are connect with protease container, and feeding device also pipeline connects fill assembly, driving device Signal connects control device, and protease container includes the detection device for detecting protease state, and detection device is in Control the connection of device signal.
By adopting the above technical scheme, detection device carries out state-detection to protease, then gives control device signal, in Control device determined upon receipt of the signals, each state under normal circumstances, give driving device signal, and then drive charging Device work, adds protease by fill assembly;To guarantee that protease is in normal condition, to reach the acidolysis of subsequent gelatin Treatment process avoids protease quality too low, impacts to gelatin treatment effeciency, or even pollute to gelatin.
Preferably, detection device includes pH detection unit, temperature detecting unit, the enzyme activity connecting respectively with control device Property detection unit and enzyme suspension concentration detecting unit;Protease needs suitable temperature and solution ph, to reach protease Optimum Working, needs the waving interval of smaller pH value and temperature, and pH detection unit can be used pH sensor, such as Line pH probe;Temperature sensor, such as online temp probe can be used in temperature detecting unit, to guarantee the optimal temperature of Protease Treatment Environment is spent, settable multiple temp probes carry out multiple spot detection to liquid of protease;Enzyme assay unit can be used electrochemistry, The method of optical physics carries out on-line checking to enzymatic activity;Strength of fluid sensor can be used to be detected for enzyme suspension detection unit; In the case where meeting liquid environment, the section pH and temperature range according to locating for protease choose corresponding detection device.
Preferably, protease container further includes the behavior control device for regulatory protein enzyme environment.
Preferably, behavior control device includes the temperature control unit connecting respectively with control device, pH control unit; Temperature control equipment can be by heating sheet, and liquid liquid exchanger regulates and controls temperature, but is unable to additive, such as refrigeration fluorine benefit High substance enters in protease liquid, based on physical method;PH control unit can pass through addition bionic acid and alkaloid pair Protease carries out pH and is controlled, and strong acid, highly basic cannot be added, with contaminating protein enzyme or the regulation and control substance that cannot be eaten.
Preferably, feeding device includes shell and the piston rod that connect with driving device, enclosure interior is provided with unidirectionally Valve.
Preferably, feeding device is connected with fill assembly by conveyance conduit.
Preferably, fill assembly includes the pressure retaining valve connecting with conveyance conduit, underlying mobile mechanism and fixation Lifting cylinder in mobile mechanism, pressure retaining valve are connected on lifting cylinder and act with lifting cylinder, one end of pressure retaining valve with Conveyance conduit connection, the other end be connected tos by connector with injection head, needle-valve be arranged between injection head and connector be used for closing and Open the circulation of protease between connector and injection head.
The accurate method for implanting of acid protease in a kind of gelatin acid hemolysis process, based on acid in above-mentioned gelatin acid hemolysis process The accurate injection device of protease, comprising the following steps:
C1: system initialization;
C2:pH detection unit, temperature detecting unit, Enzyme assay unit and enzyme suspension concentration detecting unit detect accordingly respectively State executes C3 if numerical value is in normal value, if numerical value is in outlier, executes C4;
C3: control device controls driving device, and then driving device driving feeding device adds protease by fill assembly;
C4: middle control handles abnormal numerical value, executes C2 in after treatment;
Preferably, abnormal Numerical Methods the following steps are included:
D1: when temperature value exception, temperature detecting unit gives control device signal, control device control temperature control unit into Row regulation;
D2: when pH numerical exception, pH detection unit gives control device signal, and control device control pH control unit is adjusted Control;
D3: when enzymatic activity numerical value and/or enzyme suspension solubility numerical exception, control device issues alarm signal.
The beneficial effects of the present invention are:
1, the accurate injection device of acid protease can be in real time to ring locating for protease in a kind of gelatin acid hemolysis process of the invention Border is monitored, and guarantees that protease is in optimum Working;
2, in a kind of gelatin acid hemolysis process of the invention the accurate injection device of acid protease in real time to protease local environment into Row regulation, intelligence degree are high;
3, the accurate injection device structure of acid protease is simple, functional in a kind of gelatin acid hemolysis process of the invention of the invention It is good, there is good economy and practicability.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the accurate injection device of acid protease in gelatin acid hemolysis process of the embodiment of the present invention;
Fig. 2 is the structural schematic diagram of fill assembly of the embodiment of the present invention;
Appended drawing reference:
1-driving device, 2-feeding devices, 3-protease containers, 4-detection devices, 5-conveyance conduits, 6-injections Component, 61-pressure retaining valves, 62-mobile mechanisms, 63-lifting cylinders, 64-connectors, 65-injection heads, 66-needle-valves.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that the described embodiments are merely a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Embodiment 1:
The accurate injection device of acid protease in a kind of gelatin acid hemolysis process, including driving device 1, and driven by driving device 1 Feeding device 2,2 one end of feeding device connect with protease container 3, and feeding device 2 goes back pipeline connection fill assembly 6, 1 signal of driving device connects control device, and protease container 3 includes the detection device 4 for detecting protease state, inspection Device 4 is surveyed to connect with control device signal.
By adopting the above technical scheme, detection device 4 carries out state-detection to protease, then gives control device signal, Control device determined upon receipt of the signals, each state under normal circumstances, give 1 signal of driving device, and then drive Feeding device 2 works, and adds protease by fill assembly 6;To guarantee that protease is in normal condition, to reach subsequent gelatin Acidolysis treatment process, avoid protease quality too low, gelatin treatment effeciency impacted, or even gelatin is polluted.
Preferably, detection device 4 includes the pH detection unit connecting respectively with control device, temperature detecting unit, enzyme Activity detection unit and enzyme suspension concentration detecting unit;Protease needs suitable temperature and solution ph, to reach protease Optimum Working, need the waving interval of smaller pH value and temperature, pH sensor can be used in pH detection unit, such as Online pH probe;Temperature sensor, such as online temp probe can be used in temperature detecting unit, to guarantee that Protease Treatment is optimal Temperature environment, settable multiple temp probes carry out multiple spot detection to liquid of protease;Electrification can be used in Enzyme assay unit It learns, the method for optical physics carries out on-line checking to enzymatic activity;Strength of fluid sensor can be used to be examined for enzyme suspension detection unit It surveys;In the case where meeting liquid environment, the section pH and temperature range according to locating for protease choose corresponding detection device 4.
Preferably, protease container 3 further includes the behavior control device for regulatory protein enzyme environment.
Preferably, behavior control device includes the temperature control unit connecting respectively with control device, pH control unit; Temperature control equipment can be by heating sheet, and liquid liquid exchanger regulates and controls temperature, but is unable to additive, such as refrigeration fluorine benefit High substance enters in protease liquid, based on physical method;PH control unit can pass through addition bionic acid and alkaloid pair Protease carries out pH and is controlled, and strong acid, highly basic cannot be added, with contaminating protein enzyme or the regulation and control substance that cannot be eaten.
Preferably, feeding device 2 includes shell and the piston rod connecting with driving device 1, enclosure interior is provided with list To valve.
Preferably, feeding device 2 and fill assembly 6 are connected by conveyance conduit 5.
Preferably, fill assembly 6 includes the pressure retaining valve 61 connecting with conveyance conduit 5, underlying mobile mechanism 62 With the lifting cylinder 63 being fixed in mobile mechanism 62, pressure retaining valve 61 is connected on lifting cylinder 63 and moves with lifting cylinder 63 Make, one end of pressure retaining valve 61 is connected to conveyance conduit 5, and the other end is connected to by connector 64 with injection head 65, and the setting of needle-valve 66 exists For closing and opening the circulation of protease between connector 64 and injection head 65 between injection head 65 and connector 64.
Embodiment 2:
The accurate method for implanting of acid protease in a kind of gelatin acid hemolysis process, based on acidic protein in above-mentioned gelatin acid hemolysis process The accurate injection device of enzyme, comprising the following steps:
C1: system initialization;
C2:pH detection unit, temperature detecting unit, Enzyme assay unit and enzyme suspension concentration detecting unit detect accordingly respectively State executes C3 if numerical value is in normal value, if numerical value is in outlier, executes C4;
C3: control device controls driving device 1, and then driving device 1 drives feeding device 2 to add albumen by fill assembly 6 Enzyme;
C4: middle control handles abnormal numerical value, executes C2 in after treatment;
Preferably, abnormal Numerical Methods the following steps are included:
D1: when temperature value exception, temperature detecting unit gives control device signal, control device control temperature control unit into Row regulation;
D2: when pH numerical exception, pH detection unit gives control device signal, and control device control pH control unit is adjusted Control;
D3: when enzymatic activity numerical value and/or enzyme suspension solubility numerical exception, control device issues alarm signal.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills Art field, is included within the scope of the present invention.

Claims (9)

1. the accurate injection device of acid protease in a kind of gelatin acid hemolysis process, which is characterized in that including driving device (1), and The feeding device (2) driven by driving device (1), feeding device (2) one end are connect with protease container (3), charging dress It sets (2) and goes back pipeline connection fill assembly (6), driving device (1) signal connects control device, and protease container (3) includes For detecting the detection device (4) of protease state, detection device (4) is connect with control device signal.
2. the accurate injection device of acid protease in gelatin acid hemolysis process as described in claim 1, which is characterized in that the inspection Surveying device (4) includes that the pH detection unit connecting respectively with control device, temperature detecting unit, Enzyme assay unit and enzyme are outstanding Liquid concentration detecting unit.
3. the accurate injection device of acid protease in gelatin acid hemolysis process as claimed in claim 2, which is characterized in that the egg White enzyme container (3) further includes the behavior control device for regulatory protein enzyme environment.
4. the accurate injection device of acid protease in gelatin acid hemolysis process as claimed in claim 3, which is characterized in that the shape State control device includes the temperature control unit connecting respectively with control device, pH control unit.
5. the accurate injection device of acid protease in gelatin acid hemolysis process as described in claim 1, which is characterized in that described to add Material device (2) includes shell and the piston rod that connect with driving device (1), and enclosure interior is provided with check valve.
6. the accurate injection device of acid protease in gelatin acid hemolysis process as described in claim 1, which is characterized in that described to add Expect that device (2) and fill assembly (6) are connected by conveyance conduit (5).
7. the accurate injection device of acid protease in gelatin acid hemolysis process as claimed in claim 6, which is characterized in that the note Enter component (6) to include the pressure retaining valve (61) connecting with conveyance conduit (5), underlying mobile mechanism (62) and be fixed on movement Lifting cylinder (63) in mechanism (62), pressure retaining valve (61) are connected on lifting cylinder (63) and act with lifting cylinder (63), One end of pressure retaining valve (61) is connected to conveyance conduit (5), and the other end is connected to by connector (64) with injection head (65), needle-valve (66) It is arranged between injection head (65) and connector (64) for closing and opening protease between connector (64) and injection head (65) Circulation.
8. the accurate method for implanting of acid protease in a kind of gelatin acid hemolysis process, based on any gelatin of claim 1 to 7 The accurate injection device of acid protease in acid hemolysis process, which comprises the following steps:
C1: system initialization;
C2:pH detection unit, temperature detecting unit, Enzyme assay unit and enzyme suspension concentration detecting unit detect accordingly respectively State executes C3 if numerical value is in normal value, if numerical value is in outlier, executes C4;
C3: control device controls driving device (1), and then driving device (1) driving feeding device (2) passes through fill assembly (6) Add protease;
C4: middle control handles abnormal numerical value, executes C2 in after treatment.
9. the accurate method for implanting of acid protease in gelatin acid hemolysis process as claimed in claim 8, which is characterized in that described different Constant value processing method the following steps are included:
D1: when temperature value exception, temperature detecting unit gives control device signal, control device control temperature control unit into Row regulation;
D2: when pH numerical exception, pH detection unit gives control device signal, and control device control pH control unit is adjusted Control;
D3: when enzymatic activity numerical value and/or enzyme suspension solubility numerical exception, control device issues alarm signal.
CN201910297526.3A 2019-04-15 2019-04-15 The accurate injection device of acid protease in a kind of gelatin acid hemolysis process Pending CN109971366A (en)

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Application Number Priority Date Filing Date Title
CN201910297526.3A CN109971366A (en) 2019-04-15 2019-04-15 The accurate injection device of acid protease in a kind of gelatin acid hemolysis process

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101266489A (en) * 2008-02-20 2008-09-17 福建师范大学 Laser processing long-range control process based on HTTP protocol
US20080308101A1 (en) * 2007-06-15 2008-12-18 Michael Spandorfer Drug delivery and monitoring system for a ventilator
CN102049367A (en) * 2011-01-24 2011-05-11 谢泽 Quantitative positioning glue smearing system at constant temperature and constant pressure
CN105385594A (en) * 2015-11-25 2016-03-09 陕西艾尔肤组织工程有限公司 Preparation system and preparation method of tissue engineering skin
CN107572225A (en) * 2017-09-23 2018-01-12 芜湖启尊智能科技有限公司 A kind of material conveys charging device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080308101A1 (en) * 2007-06-15 2008-12-18 Michael Spandorfer Drug delivery and monitoring system for a ventilator
CN101266489A (en) * 2008-02-20 2008-09-17 福建师范大学 Laser processing long-range control process based on HTTP protocol
CN102049367A (en) * 2011-01-24 2011-05-11 谢泽 Quantitative positioning glue smearing system at constant temperature and constant pressure
CN105385594A (en) * 2015-11-25 2016-03-09 陕西艾尔肤组织工程有限公司 Preparation system and preparation method of tissue engineering skin
CN107572225A (en) * 2017-09-23 2018-01-12 芜湖启尊智能科技有限公司 A kind of material conveys charging device

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