CN109970880A - A kind of preparation method of ginseng leaf polyose - Google Patents
A kind of preparation method of ginseng leaf polyose Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Materials Engineering (AREA)
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Abstract
The present invention discloses a kind of preparation method of ginseng leaf polyose, including pre-treatment step, defatting step, homogenizing step and concentration centrifugation step.The preparation method of ginseng leaf polyose of the present invention not only contributes to the comprehensive utilization of natural resources of Chinese medicinal materials, reduces economic cost, high efficiency extraction effective component of chinese medicine, and the application to natural resources of Chinese medicinal materials in livestock and poultry cultivation field is also of great significance.
Description
Technical field
The present invention relates to the preparation methods of herbal medicine pharmaceutical field more particularly to ginseng leaf polyose.
Background technique
In China, ginseng is considered as King of Herbs.Shennong's Herbal recite " ginseng, sweet in flavor slightly cold, main tonifying five zang organs,
Soothe the nerves determine soul, and stop palpitate with fear removes pathogenic factor, improving eyesight, happy intelligence development.Long term usage, macrobiosis of making light of one's life by commiting suicide.One famous person rank, a ghost lid.It is raw
Mountain valley." this illustrates that ginseng is used as medicine in existing thousands of years of the history of China.Ginseng is rich in a variety of active ingredients, such as ginsenoside, more
Sugar, vitamin, volatile oil, microelement, amino acid etc..It was verified that ginseng is high-grade nourishing treasure, immunity of organism can be enhanced
Function, it is anti-oxidant, it is antitumor, central nervous system is adjusted, the extensive effects such as cardiac function are improved.Studies have shown that folium panacis japonici cum caule
Effective component and pharmacological action and ginseng it is very much like, all have for tumour, hepatitis, the nervous system disease etc. and good control
Therapeutic effect can enhance the resistance of body.According to external recent report, the polysaccharide component in folium panacis japonici cum caule can promote body macrophage thin
Born of the same parents' Fc expression of receptor generates IL-6, participates in the adjusting of body immune system.
In addition, ginseng is perennial herb, ginseng needs 6~15 years growth times, and folium panacis japonici cum caule can be annual
Picking in due course, abundance.And traditional method for extracting polysaccharide is mostly used hot water extraction technique, generally requiring 80 DEG C or more decocts, high
Temperature is easily destroyed polysaccharide structures, and the height that consumes energy.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of preparation method of ginseng leaf polyose, not only contributes to
The comprehensive utilization of natural resources of Chinese medicinal materials reduces economic cost, high efficiency extraction effective component of chinese medicine, to natural resources of Chinese medicinal materials in livestock and poultry cultivation field
Application be also of great significance.
Achieving the object of the present invention can be reached by adopting the following technical scheme that:
A kind of preparation method of ginseng leaf polyose, comprising the following steps:
Pre-treatment step: weighing ginseng leaf raw material, cleans, and dries, and crushes, and sieving obtains Ginseng Leaf；
Defatting step: taking Ginseng Leaf, by Ginseng Leaf degreasing；
Homogenizing step: distillation water homogenisation is added into the Ginseng Leaf after degreasing, obtains folium panacis japonici cum caule's polysaccharide extraction liquid；
Concentration centrifugation step: taking folium panacis japonici cum caule's polysaccharide extraction liquid, is concentrated under reduced pressure, is added dehydrated alcohol after letting cool, after standing from
For gains in depth of comprehension to sediment, taking precipitate freeze-drying obtains ginseng leaf polyose.
Further, in pre-treatment step, the temperature of drying is 40-60 DEG C, and the time of drying is 12-24 hours.
Further, in pre-treatment step, sieving uses the sieve of 60-80 mesh, and the sieve of 60-80 mesh is conducive to high-pressure homogeneous
Sufficiently broken crude drug cell, conducive to the extraction of herbal polysaccharide
Further, in defatting step, degreasing use mass concentration for 95% alcohol reflux degreasing twice, 2 is small every time
When, ethyl alcohol volume is 5 times of Ginseng Leaf.Degreasing can make Chinese medicine material powder removing lipid, pigment and small molecular weight impurity, make
Last polyose character is uniform, and stability is more preferable.
Further, in homogenizing step, the solid-liquid ratio of Ginseng Leaf and distilled water is 1:15~1:25 (W/V).Such as material
Liquor ratio is too low, and polysaccharide cannot get effective homogeneous, and solid-liquid ratio is excessively high, and polysaccharide cannot effectively dissolve.
Further, in homogenizing step, the pressure of homogeneous is 600~1200bar, and homogenization cycles are 1~3 time.Homogeneous pressure
Power is selected above 600bar, can effectively be crushed folium panacis japonici cum caule's eucaryotic cell structure, sufficiently extracts herbal polysaccharide ingredient.Homogenization cycles choose 1
~3 times, be to influence active polysaccharide because temperature rising is too fast when homogenization cycles excessively be easy to cause homogeneous.
Further, it is concentrated in centrifugation step, the volumetric concentration that dehydrated alcohol is added to ethyl alcohol is 80%.
Further, it is concentrated in centrifugation step, dwell temperature is 4 DEG C, and time of repose is 24 hours.
Compared with prior art, the beneficial effects of the present invention are:
1, the preparation method of ginseng leaf polyose of the invention, using orthogonal experiment to folium panacis japonici cum caule's Polysaccharide Extraction
It optimizing, for the preparation method provided using folium panacis japonici cum caule as raw material, resource utilization is high, and it is at low cost, not only contribute to natural resources of Chinese medicinal materials
Comprehensive utilization, reduce economic cost, high efficiency extraction effective component of chinese medicine, to natural resources of Chinese medicinal materials livestock and poultry cultivation field application
It is of great significance, also overcomes conventional hot water and extract the disadvantage that polyoses content is low, energy consumption is high；
2, the principle for the high pressure homogenizer that homogenizing step of the invention uses for, material is sucked and is pressurizeed by plunger pump,
After the current restricting slit of specific width, the material of instantaneous loss of pressure is sprayed with high flow velocity (1000~1500m/s), and collision exists
It collides on ring, generates three kinds of hole, shock and shearing effects, make material average grain diameter up to 1 μm hereinafter, high pressure homogenization technique is
The new technology of effective component of chinese medicine is extracted, for traditional Chinese medicine extraction mainly based on diffusion extraction, high-pressure homogeneous process can make cell wall
It destroys, solvent is quickly spread, thus high efficiency extraction effective component of chinese medicine, high-pressure homogeneous process is to touch material with high flow velocity
It hits, by folium panacis japonici cum caule's cell wall damage, makes average grain diameter up to 1 μm hereinafter, quickly spreading by solvent is fully dissolved out polysaccharide；
3, the ginseng leaf polyose that preparation method of the invention obtains has antioxidant activity effect, can enhance chicken growth performance
And immunity, the use of antibiotics is reduced, there is development prospect in livestock and poultry cultivation field.
Detailed description of the invention
Fig. 1 is influence schematic diagram of the solid-liquid ratio to folium panacis japonici cum caule's polysaccharide extract rate；
Fig. 2 is influence schematic diagram of the homogenization pressure to folium panacis japonici cum caule's polysaccharide extract rate；
Fig. 3 is influence schematic diagram of the homogenization cycles to folium panacis japonici cum caule's polysaccharide extract rate.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention:
Embodiment 1:
A kind of preparation method of ginseng leaf polyose, comprising the following steps:
Weigh ginseng leaf raw material, clean, 60 DEG C continuous drying 12 hours, crush, cross 60 meshes, with the 95% of 5 times of volumes
Alcohol reflux degreasing 2 times, each 2h, residue and drying are separated, obtains the ginseng of removal lipid, pigment and small molecular weight impurity ingredient
Leaf powder is added after distilled water through high pressure homogenization method polysaccharide, changes solid-liquid ratio and carry out single factor experiment, homogenization pressure
900bar, homogenization cycles 2 times, extracting solution is concentrated under reduced pressure, and it is 80% that dehydrated alcohol to determining alcohol is added after letting cool, and 4 DEG C quiet
It sets 24 hours, centrifugation obtains sediment, takes the cold east of precipitating dry, obtains ginseng leaf polyose.Calculate folium panacis japonici cum caule's polysaccharide extract rate.
Embodiment 2:
A kind of preparation method of ginseng leaf polyose, comprising the following steps:
Weigh ginseng leaf raw material, clean, 60 DEG C continuous drying 12 hours, crush, cross 60 meshes, with the 95% of 5 times of volumes
Alcohol reflux degreasing 2 times, each 2h, residue and drying are separated, obtains the ginseng of removal lipid, pigment and small molecular weight impurity ingredient
Leaf powder is added after distilled water through high pressure homogenization method polysaccharide, changes homogenization pressure and carry out single factor experiment, solid-liquid ratio 1:20
(W/V), homogenization cycles 2 times.Extracting solution is concentrated under reduced pressure, and it is 80% that dehydrated alcohol to determining alcohol is added after letting cool, 4 DEG C of standings
24 hours, centrifugation obtained sediment, takes the cold east of precipitating dry, obtains ginseng leaf polyose.Calculate folium panacis japonici cum caule's polysaccharide extract rate.
Embodiment 3:
A kind of preparation method of ginseng leaf polyose, comprising the following steps:
Weigh ginseng leaf raw material, clean, 60 DEG C continuous drying 12 hours, crush, cross 60 meshes, with the 95% of 5 times of volumes
Alcohol reflux degreasing 2 times, each 2h, residue and drying are separated, obtains the ginseng of removal lipid, pigment and small molecular weight impurity ingredient
Leaf powder is added after distilled water through high pressure homogenization method polysaccharide, changes homogenization cycles and carry out single factor experiment, solid-liquid ratio 1:20
(W/V), homogenization pressure 900bar.Extracting solution is concentrated under reduced pressure, after letting cool be added dehydrated alcohol to determining alcohol be 80%, 4 DEG C
24 hours are stood, centrifugation obtains sediment, takes the cold east of precipitating dry, obtains ginseng leaf polyose.Calculate folium panacis japonici cum caule's polysaccharide extract rate.
Comparative example 1:
It is as follows that Hot water extraction extracts ginseng leaf polyose method:
Weigh ginseng leaf raw material, clean, 60 DEG C continuous drying 12 hours, crush, cross 60 meshes, with the 95% of 5 times of volumes
Alcohol reflux degreasing 2 times, each 2h, residue and drying are separated, obtains the ginseng of removal lipid, pigment and small molecular weight impurity ingredient
Leaf powder is added purified water by solid-liquid ratio 1:20 (g/mL), is placed in 90 DEG C of water-baths, extracts twice, each 2h, combined extract,
Extracting solution is concentrated under reduced pressure, and it is 80% that dehydrated alcohol to determining alcohol is added after letting cool, and 4 DEG C stand 24 hours, and centrifugation is sunk
Starch takes the cold east of precipitating dry, obtains ginseng leaf polyose.The folium panacis japonici cum caule's polysaccharide extract rate obtained through traditional aqueous extraction-alcohol precipitation technology is
10.17%.
Effect assessment and performance detection
One, optimum extraction process screens
Weigh ginseng leaf raw material, clean, 60 DEG C continuous drying 12 hours, crush, cross 60 meshes, with the 95% of 5 times of volumes
Alcohol reflux degreasing 2 times, each 2h, residue and drying are separated, obtains the ginseng of removal lipid, pigment and small molecular weight impurity ingredient
Leaf powder is added after distilled water through high pressure homogenization method polysaccharide, determines ginseng leaf polyose optimum extraction condition with orthogonal experiment,
With solid-liquid ratio (A), homogenization pressure (B), homogenization cycles (C) are investigation factor, using polysaccharide extract rate as inspection target, using L9
(33) orthogonal arrage tested (table 1), screen ginseng leaf polyose optimum extraction process.
1 orthogonal test factor level of table
Extracting solution after high-pressure homogeneous is concentrated under reduced pressure, after letting cool be added dehydrated alcohol to determining alcohol be 80%, 4 DEG C
24 hours are stood, centrifugation obtains sediment, takes the cold east of precipitating dry, obtains ginseng leaf polyose.High-pressure homogeneous orthogonal test scheme
And it the results are shown in Table 2
2 orthogonal experiments of table
As shown in Table 2, using polysaccharide extract rate as inspection target, the size of very poor R shows that each factor primary and secondary is
Solid-liquid ratio (A) > homogenization cycles (C) > homogenization pressure (B).Using polysaccharide extract rate as the orthogonal test variance analysis knot of inspection target
Fruit is as shown in table 3.The results of analysis of variance shows that solid-liquid ratio (A) and homogenization cycles (C) have significantly the recovery rate of ginseng leaf polyose
It influences (p < 0.05).It is intuitively analyzed in conjunction with table 2 it is found that high pressure homogenization technique is used to extract the optimization technique of ginseng leaf polyose as material
Liquor ratio 1:20 (W:V), homogenization pressure 900bar, homogenization cycles are 3 times, and the recovery rate of ginseng leaf polyose is 16.59%.
3 orthogonal test the results of analysis of variance of table
Two, the influence of folium panacis japonici cum caule's polysaccharide extract rate
Influence Fig. 1 shows solid-liquid ratio to folium panacis japonici cum caule's polysaccharide extract rate；Fig. 2 indicates homogenization pressure to folium panacis japonici cum caule's Polyose extraction
The influence of rate；Fig. 3 indicates influence of the homogenization cycles to folium panacis japonici cum caule's polysaccharide extract rate.
As shown in Figure 1, when solid-liquid ratio is 1:20 (W/V), ginseng leaf polyose starves recovery rate highest more；Solid-liquid ratio is 1:
15, when 1:20 and 1:25 (W/V), the recovery rate of ginseng leaf polyose is above 10%；When being added, solvent is excessive, and polysaccharide cannot have
Homogeneous is imitated, when solvent is very few, polysaccharide cannot effectively dissolve, and cause polysaccharide extract rate relatively low.Therefore solid-liquid ratio preferred scope
For 1:15~1:25 (W/V).
As shown in Figure 2, when homogenization pressure is greater than 600bar, polysaccharide is effectively extracted.
From the figure 3, it may be seen that when homogenization cycles are that 1~5 polysaccharide extract rate is above 10%, since homogenization cycles can excessively be made
Rapidly at heating, polysaccharide loss is caused, and time-consuming for preparation, therefore preferably high-pressure homogeneous number is 1~3 time.
Three, ginseng leaf polyose detection method
Ginseng leaf polyose detection method is as follows,
The preparation of reference substance solution: accurately weighed galacturonic acid reference substance 50mg is placed in 100ml volumetric flask, adds water
Making to dissolve, be diluted with water to scale, shakes up, precision measures 10ml, and it sets in 100ml volumetric flask, is diluted with water to scale, shake up,
Be made the solution of every 1ml 50 μ g containing galacturonic to get；
The preparation of test solution: taking this product 500mg, accurately weighed, sets in 100ml measuring bottle, adds appropriate amount of water, ultrasound makes molten
Solution, is diluted with water to scale, shakes up, and filters, and precision measures 10ml, sets in 100ml volumetric flask, is diluted with water to scale, shakes up,
To obtain the final product；
Measuring method: precision measures reference substance solution and each 1ml of test solution, sets in stuffed conical flask respectively, sets ice bath
Middle cooling 10 minutes is taken out, and 0.025mol/L borax sulfuric acid solution 2ml is added in precision, is mixed, is set in water-bath and heat 10 minutes,
5 minutes cooling in ice bath, taking-up is set immediately, and precision 0.125% carbazole ethanol solution 0.4ml of addition is mixed, set in water-bath
Heating 10 minutes is set 5 minutes cooling in ice bath immediately.Blank test correction is done simultaneously, it is (attached according to UV-VIS spectrophotometry
Record 0401) test, at 530nm wavelength measure absorbance, calculate to get.
Folium panacis japonici cum caule's polyoses content (in terms of galacturonic acid) obtained by the embodiment of the present invention 4 and comparative example 1 is respectively
23.10% and 18.09%, illustrate that the effect of the preparation method of extraction ginseng leaf polyose of the invention is extracted better than conventional hot water
Method.
Five, scavenging effect of the ginseng leaf polyose to DPPH free radical
Take respectively various concentration folium panacis japonici cum caule's polysaccharide sample (0.1mg/mL, 0.2mg/mL, 0.6mg/mL, 0.8mg/mL,
1.0mg/mL) 2mL is added 2mL 0.2mmol/L DPPH solution (dehydrated alcohol is as solvent), mixes, keeps away at room temperature
Light stands 30min, and 2mL DPPH solution is added as blank using 2mL distilled water, absorbance A value is measured at 517nm wavelength.According to
The clearance rate of formula (1) calculating DPPH free radical.
In formula, AcThe absorbance value of 2mL DPPH solution, A are added for 2mL distilled wateri2mL is added for 2mL polysaccharide solution
The absorbance value of DPPH solution.
Ginseng leaf polyose shows dosage effect to the scavenging effect of DPPH free radical within the scope of a certain concentration, i.e., with
The increase of folium panacis japonici cum caule's polysaccharide concentration and improve.When folium panacis japonici cum caule's polysaccharide concentration is 1.0mg/mL, the clearance rate of DPPH free radical is
38.61%.
Five, scavenging effect of the ginseng leaf polyose to hydroxyl radical free radical
1.0mL 0.15mol/L phosphate buffer solution (pH=7.4), 0.2mL 260mg/ are sequentially added in 10mL test tube
L safranine T solution, 3% hydrogen peroxide of 0.5mL, 0.5mL6mmol/L EDTA-Na+-Fe2+, it is then respectively adding various concentration
Folium panacis japonici cum caule's polysaccharide sample (0.1mg/mL, 0.5mg/mL, 1.0mg/mL, 2.0mg/mL, 3.0mg/mL) 1mL, is placed in 37 for test tube
DEG C water bath with thermostatic control 30min measures absorbance A value at 520nm wavelength.The clearance rate of hydroxyl radical free radical is calculated according to formula (2).
In formula, A0For sample solution plus EDTA-Na+-Fe2+The absorbance value of solution, A1Sample is substituted for isometric distilled water
The absorbance value of solution, A2Sample solution and EDTA-Na are substituted for isometric distilled water+-Fe2+The absorbance value of solution.
Ginseng leaf polyose is improved in the scavenging effect to hydroxyl radical free radical with the increase of concentration.When folium panacis japonici cum caule's polysaccharide concentration
When for 3.0mg/mL, the clearance rate of hydroxyl radical free radical is 43.74%.
Six, the influence of folium panacis japonici cum caule's Polysaccharides in Chicken immune function
When 1 age in days Lingnanhuang broilers are raised to 7 age in days, chooses the uniform healthy chicken 100 of weight and be only randomly divided into 5 groups, often
Group 20, the respectively the 1st, 2,3,4,5 test groups.In 7 age in days, newcastle disease virus is carried out to the 2nd~6 group of experimental chicken
(LaSota plants)-bird flu (H9 hypotype) bivalent inactivated vaccine is immune, and the 1st group is blank control group (not giving drug), and the 2nd group is
Positive control veterinary drug group (addition control 0.2 ‰ astragalus polyose powder of veterinary drug in drinking-water), the 3rd group (is added for high dose group in drinking-water
0.2 ‰ folium panacis japonici cum caule's polysaccharide powders), the 4th group is low dose group (0.1 ‰ folium panacis japonici cum caule's polysaccharide powders are added in drinking-water).7 ages in days start to throw
Medicine, continuous dispensing 7 days, experimental period 28 days.Experimental chicken is freely eaten, drinks water during test.
3 each group experimental chicken growth performance situation (n=20) of table
As shown in Table 3, in 35 age in days of experimental chicken, the weight of dosing group (the 2nd, 3,4 group) experimental chicken is significantly higher than the 1st group
(p < 0.05), group difference is not significant.When off-test, the experimental chicken average daily gain for adding medicine group (the 2nd, 3,4 group) is equal
It is significantly higher than not dosing group (the 1st group), and the average daily gain of the 3rd group of test chicken is significantly higher than the 2nd and the 3rd group.The result shows that
Ginseng leaf polyose is added in experimental chicken drinking-water, test chicken can be remarkably promoted and grow (p < 0.05), and 0.2 ‰ ginseng leaf polyoses
Effect is better than 0.2 ‰ astragalus polyoses and 0.1 ‰ ginseng leaf polyoses.
The influence (n=10) of 4 folium panacis japonici cum caule's Polysaccharides in Chicken newcastle disease potency of table
The influence test result of folium panacis japonici cum caule's Polysaccharides in Chicken antibody titres to newcastle disease virus is shown in table 4, in 7 age in days of experimental chicken,
Each group difference is not significant；In 21 ages in days and 35 age in days, the 2nd~4 group of experimental chicken antibody titres to newcastle disease virus is all remarkably higher than the 1st group
(p < 0.05), but dosing group difference is not significant.Test result shows continuously to add 0.1 ‰ and 0.2 ‰ in experimental chicken drinking-water
Ginseng leaf polyose, in test, the later period can significantly improve experiment antibody IgY against chicken Newcastle Disease horizontal, effect and 0.2 ‰ astragalus polyoses
It is similar.
5 folium panacis japonici cum caule's Polysaccharides in Chicken bird flu H of table9The influence (n=10) of antibody titer
Folium panacis japonici cum caule's Polysaccharides in Chicken bird flu H in table 59The influence test result of antibody titer is shown, in 7 age in days of experimental chicken
When, each group difference is not significant；In 21 age in days, the 3rd group is significantly higher than its excess-three group (p < 0.05)；In 35 age in days, the 2nd~4
Group experiment avian influenza H9Antibody titer is all remarkably higher than the 1st group (p < 0.05), but dosing group difference is not significant.Test result
Show continuously to add 0.1 ‰ ginseng leaf polyoses in experimental chicken drinking-water, the phase can significantly improve experiment avian influenza H after experiment9
Antibody level, effect are similar to 0.2 ‰ astragalus polyoses；0.2 ‰ ginseng leaf polyoses are continuously added in experimental chicken drinking-water, are being tried
Experiment avian influenza H can be significantly improved by testing the middle and later periods9Antibody level, effect are better than astragalus polyose group.
The result shows that folium panacis japonici cum caule's Polysaccharides in Chicken that preparation method of the invention obtains only increases weight, antibody IgY against chicken Newcastle Disease is horizontal
With bird flu H9Antibody level has preferable reinforcing effect, and market application prospect is wide.
To sum up, the preparation method of ginseng leaf polyose of the invention, using orthogonal experiment to folium panacis japonici cum caule's polysaccharide extracting process
Condition optimizes, and for the preparation method provided using folium panacis japonici cum caule as raw material, resource utilization is high, at low cost, not only contributes to Chinese medicine
The comprehensive utilization of resource, reduces economic cost, and high efficiency extraction effective component of chinese medicine answers natural resources of Chinese medicinal materials in livestock and poultry cultivation field
With being also of great significance, also overcomes conventional hot water and extract the disadvantage that polyoses content is low, energy consumption is high.
For those skilled in the art, it can make other each according to the above description of the technical scheme and ideas
Kind is corresponding to be changed and deforms, and all these change and deform the protection model that all should belong to the claims in the present invention
Within enclosing.
Claims (8)
1. a kind of preparation method of ginseng leaf polyose, which comprises the following steps:
Pre-treatment step: weighing ginseng leaf raw material, cleans, and dries, and crushes, and sieving obtains Ginseng Leaf；
Defatting step: taking Ginseng Leaf, by Ginseng Leaf degreasing；
Homogenizing step: distillation water homogenisation is added into the Ginseng Leaf after degreasing, obtains folium panacis japonici cum caule's polysaccharide extraction liquid；
Concentration centrifugation step: taking folium panacis japonici cum caule's polysaccharide extraction liquid, is concentrated under reduced pressure, dehydrated alcohol is added after letting cool, is centrifuged after standing
To sediment, taking precipitate freeze-drying obtains ginseng leaf polyose.
2. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in pre-treatment step, the temperature of drying
Degree is 40-60 DEG C, and the time of drying is 12-24 hours.
3. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in pre-treatment step, sieving is used
The sieve of 60-80 mesh.
4. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in defatting step, degreasing uses matter
Measure the alcohol reflux degreasing that concentration is 95% twice, 2 hours every time, ethyl alcohol volume was 5 times of Ginseng Leaf.
5. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in homogenizing step, Ginseng Leaf
Solid-liquid ratio with distilled water is 1:15~1:25.
6. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in homogenizing step, the pressure of homogeneous
For 600~1200bar, homogenization cycles are 1~3 time.
7. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in concentration centrifugation step, nothing is added
The volumetric concentration of water-ethanol to ethyl alcohol is 80%.
8. the preparation method of ginseng leaf polyose as described in claim 1, which is characterized in that in concentration centrifugation step, stand temperature
Degree is 4 DEG C, and time of repose is 24 hours.
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| US20100247581A1 (en) * | 2007-12-07 | 2010-09-30 | Abrahamse Salomon Leendert | Edible product having an immunostimulating effect |
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| CN101002858A (en) * | 2006-12-19 | 2007-07-25 | 戴甲木 | Medicine composition for enhancing immunity, and its preparing method and use |
| CN101157731A (en) * | 2007-11-06 | 2008-04-09 | 吉林省宏久生物科技股份有限公司 | Preparation method for low agriculture remanet panax ginseng and American ginseng polysaccharide extractive |
| US20100247581A1 (en) * | 2007-12-07 | 2010-09-30 | Abrahamse Salomon Leendert | Edible product having an immunostimulating effect |
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