CN109966347A - A kind of extraction process of white pond lily flower extract - Google Patents
A kind of extraction process of white pond lily flower extract Download PDFInfo
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- CN109966347A CN109966347A CN201910252266.8A CN201910252266A CN109966347A CN 109966347 A CN109966347 A CN 109966347A CN 201910252266 A CN201910252266 A CN 201910252266A CN 109966347 A CN109966347 A CN 109966347A
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- filtrate
- filter residue
- white pond
- extraction process
- pond lily
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- 240000006665 Nymphaea gigantea Species 0.000 title claims abstract description 37
- 238000000605 extraction Methods 0.000 title claims abstract description 29
- 239000000284 extract Substances 0.000 title claims abstract description 22
- 239000000706 filtrate Substances 0.000 claims abstract description 59
- 229930003944 flavone Natural products 0.000 claims abstract description 51
- 235000011949 flavones Nutrition 0.000 claims abstract description 51
- -1 flavone compound Chemical class 0.000 claims abstract description 50
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 48
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000008367 deionised water Substances 0.000 claims abstract description 23
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 23
- 239000012043 crude product Substances 0.000 claims abstract description 21
- 239000002253 acid Substances 0.000 claims abstract description 18
- 238000009835 boiling Methods 0.000 claims abstract description 16
- 238000002203 pretreatment Methods 0.000 claims abstract description 13
- 238000000967 suction filtration Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 6
- 230000007935 neutral effect Effects 0.000 claims abstract description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 20
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 claims description 10
- 229940092714 benzenesulfonic acid Drugs 0.000 claims description 10
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 10
- QDRKDTQENPPHOJ-UHFFFAOYSA-N sodium ethoxide Chemical group [Na+].CC[O-] QDRKDTQENPPHOJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 4
- 239000011800 void material Substances 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 230000007062 hydrolysis Effects 0.000 description 10
- 238000006460 hydrolysis reaction Methods 0.000 description 10
- 230000006872 improvement Effects 0.000 description 9
- 241000209490 Nymphaea Species 0.000 description 8
- 235000016791 Nymphaea odorata subsp odorata Nutrition 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 229930003935 flavonoid Natural products 0.000 description 6
- 150000002215 flavonoids Chemical class 0.000 description 6
- 235000017173 flavonoids Nutrition 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 150000007524 organic acids Chemical class 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000001953 recrystallisation Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 240000002853 Nelumbo nucifera Species 0.000 description 3
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 3
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000002213 flavones Chemical class 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical class [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- OVSQVDMCBVZWGM-IDRAQACASA-N Hirsutrin Natural products O([C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](CO)O1)C1=C(c2cc(O)c(O)cc2)Oc2c(c(O)cc(O)c2)C1=O OVSQVDMCBVZWGM-IDRAQACASA-N 0.000 description 1
- FVQOMEDMFUMIMO-UHFFFAOYSA-N Hyperosid Natural products OC1C(O)C(O)C(CO)OC1OC1C(=O)C2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 FVQOMEDMFUMIMO-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- GXMWXESSGGEWEM-UHFFFAOYSA-N isoquercitrin Natural products OCC(O)C1OC(OC2C(Oc3cc(O)cc(O)c3C2=O)c4ccc(O)c(O)c4)C(O)C1O GXMWXESSGGEWEM-UHFFFAOYSA-N 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- OVSQVDMCBVZWGM-QSOFNFLRSA-N quercetin 3-O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-QSOFNFLRSA-N 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Polymers & Plastics (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses a kind of extraction processes of white pond lily flower extract, include the following steps: S1: the petal pre-treatment of white pond lily；S2: primary to decoct；Deionized water is added to decoct on the petal after pre-treatment, and the mass ratio of deionized water and petal is (5-7): 1；S3: alkaline environment is adjusted；To alkaline matter is added in the liquid after S2 boiling, so that the pH of system is adjusted to 8-10, and system is made to continue the 20-40min that boils under the conditions of this pH；S4: first to filter；Treated that liquid filters at once by S3, takes filtrate, and be labeled as A filtrate；S5: weak acid environment is adjusted；After A filtrate is heated to 60-80 DEG C, the pH of A filtrate is adjusted to 4-5, is stirred evenly, and stand 8-10h；S6: last filters；Last time suction filtration is carried out to S5 treated liquid, and filter residue is washed till neutrality；S7: S6 is washed till to neutral filter residue and drying；The crude product of flavone compound is arrived after S7.By using above-mentioned technique, flavone compound can be extracted from the petal of white pond lily.
Description
Technical field
The present invention relates to the technical fields of extracting flavonoids technique, more specifically, it relates to a kind of white pond lily flower extract
Extraction process.
Background technique
Water lily is also known as lotus.The rhizome portion of water lily is divided into lotus rhizome, and lotus rhizome hypertrophy more piece is mostly white to brown.Water lily starch
Taste is slightly sweet, tastes crisp and palatable.It can eat raw, can also boil and cook.There is quite high medical value, its each position is equal
For treasured.Water lily is processed into water lily powder, there are many effects, than if any the effect of preventing internal haemorrhage.Water lily it is many kinds of,
According to different purposes, so that it may separate different classifications.
White pond lily, also known as European white pond lily are a kind of water lily platymisciums from Europe.Petal white, calyx green,
Tip white.Anther is faint yellow, slightly has villus.Leaf table green, blade back pistac, leaf margin have red streak.White pond lily
The main chemical compositions at petal position are flavone compounds and the small molecule phenols such as rutin, Quercetin, kaempferol, isoquercitrin
Substance.
Flavone compound is widely present in nature, is a kind of important natural organic-compound, is a kind of with more
The bioactive substance of kind physiological activity is a kind of very strong antioxidant, can effectively remove the oxygen radical of physical efficiency.Flavones
Class compound can also improve blood circulation, reduce cholesterol, greatly reduce the disease incidence of cardiovascular and cerebrovascular disease, can also change
The symptom of mercy cranial vascular disease.It can increase the toughness of blood vessel, reduce blood viscosity, have preventing hypertension, apoplexy
Etc. diseases effect.
But it is domestic at present very few about the research for spending middle extraction flavone compound from white pond lily, and technology is not
It is mature；Therefore, flavone compound is extracted in white pond lily is spent in fields such as medical treatment, food with very big application potential and again
The meaning wanted.
Summary of the invention
The purpose of the present invention is to provide a kind of extraction processes of white pond lily flower extract, can be from the petal of white pond lily
Extract flavone compound.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of extraction process of white pond lily flower extract, includes the following steps:
S1: pre-treatment is carried out to the petal of white pond lily；
S2: primary to decoct；Add deionized water to decoct on the petal after pre-treatment, and adds the quality of deionized water and petal
Than for (5-7): 1；
S3: alkaline environment is adjusted；Alkaline matter is added in liquid after boiling to S2, so that the pH of system is adjusted to 8-10,
And system is made to continue the 20-40min that boils under conditions of this pH；
S4: first to filter；To S3, treated that liquid filters at once, takes filtrate, and be labeled as A filtrate；
S5: weak acid environment is adjusted；After A filtrate is heated to 60-80 DEG C, the pH of A filtrate is adjusted to 4-5, is then stirred
Uniformly, and 8-10h is stood；
S6: last filters；Last time suction filtration is carried out to S5 treated liquid, and filter residue is washed till neutrality；
S7: dry；S6 is washed till to neutral filter residue and drying；
The crude product of flavone compound is arrived after S7.
Flavone compound because having phenolic hydroxyl group more in molecule, so aobvious acidity, dissolves in alkaline aqueous solution, pyridine, first
In amide and dimethylformamide.First the petal of white pond lily is decocted using deionized water, so that each group in petal
It knits heated and obtains energy, alkaline matter is then added in system, so that the pH of system is adjusted to 8-10, so that white
Some flavone compounds in the petal of water lily are dissolved out from petal, are dissolved in the aqueous solution of alkalinity.Then it filters,
Filtrate is taken, and the pH value of filtrate is adjusted to weak acid environment.
The aobvious alkalinity of flavone compound, and the pH value of system is adjusted to acid environment, so that flavonoids
Closing object can not continue to be dissolved under acid environment, and the form finally also precipitated stays in the bottom of aqueous solution；On the other hand, flavones
1- oxygen atom in class compound on γ-pyranoid ring because there is unshared electronics pair, therefore shows as faint alkalinity, can be with
Strong inorganic acid etc. generates oxonium salt, but the oxonium salt generated is extremely unstable, and can be dissolved in water, thus can not be in subsequent
Flavone compound is separated from system.Finally its sediment is carried out to be eluted to neutrality, is then dried to obtain Huang
The crude product of ketone compounds.
As a further improvement of the present invention, added regulator is sodium ethoxide in the S3 tune alkaline environment.
By using above-mentioned technical proposal, it is sodium hydroxide and ethyl alcohol that sodium ethoxide, which meets water decomposition, and sodium hydroxide can be adjusted
PH to 8-10 in system ensure that flavone compound can be good at being dissolved in alkaline environment, to facilitate extraction.And second
Alcohol is originally the extractant of good natural plants, has extremely strong penetrability to the cell wall of plant, so as to by petal
The flavone compound of depths extracts, to improve the yield of last flavone compound.
As a further improvement of the present invention, after S4 suction filtration processing for the first time, the filter residue of this step is added into deionization again
Water decocts 20-30min, and is filtered at once, and filtrate is taken, and is labeled as B filtrate；Then A filtrate is uniformly mixed with B filtrate,
Labeled as C filtrate.
By using above-mentioned technical proposal, by the decoction again and extraction to filter residue, so that discarded position
In flavone compound be utilized again, the yield for reducing the waste of resource, while making flavone compound last again
It improves.
As a further improvement of the present invention, the S4 for the first time suction filtration processing after, by the filter residue of this step add from
Sub- water is heated to boiling, and then adds sodium carbonate, then carries out secondary decoction, after decocting 20-30min, is taken out again at once
Filter, takes filtrate, is labeled as D filtrate；Then A filtrate mutually mixes with D filtrate, is labeled as E filtrate.
By using above-mentioned technical proposal, sodium carbonate liquor shows alkalescent, but after sodium carbonate is added into solution,
Hydrolysis can occur for sodium carbonate in aqueous solution, and hydrolysis belongs to the endothermic reaction, so with the raising of solution temperature,
Be conducive to the generation of hydrolysis, so that, hydrolysis degree increase mobile to hydrolysis direction is balanced, the alkalinity enhancing of solution,
The carbonic acid amount generated simultaneously is fewer, cannot decompose to give off carbon dioxide.To which the compound of the flavonoids in filter residue can be more
More is dissolved in liquid system, finally makes the yield of flavone compound higher.
As a further improvement of the present invention, void is towards adding deionized water, deionized water in filter residue after S4 is filtered for the first time
The mass ratio of added quality and filter residue is (3-5): 1.
As a further improvement of the present invention, before the S2 is once decocted, addition 1-2 is broken in the system for needing to decoct
Tile.
Prevent system from bumping occurs during reaction by the broken tile of addition by using above-mentioned technical proposal, from
And make the process of preparation safer.
As a further improvement of the present invention, the pH of A filtrate is adjusted using benzene sulfonic acid in the S5 tune weak acid environment
To 4-5.
By using above-mentioned technical proposal, benzene sulfonic acid belongs to the middle strong acid of organic acid, enables to change using micro
PH value in variant system, to accelerate the process of adjusting, on the other hand, this flavine belongs to organic acid, so as to avoid
Flavone compound generates unstable oxonium salt, increases the yield of flavone compound.
As a further improvement of the present invention, filter residue of the S7 after dry is recrystallized using boiling water, and 60-90 DEG C into
Row drying obtains the crude product of flavone compound.
By using above-mentioned technical proposal, the means recrystallized by boiling water, so that the purity of flavone compound
It is higher.
In conclusion the advantages of the present invention are:
1, by S1 to S7 the step of can extract the flavone compound in the petal of white pond lily, thus
The fields such as medical treatment, food have very big application potential and important meaning, while extraction process comparative maturity, facilitate work people
Member is operated；
2, sodium carbonate liquor shows alkalescent, but after sodium carbonate is added into solution, sodium carbonate meeting in aqueous solution
Hydrolysis occurs, and hydrolysis belongs to the endothermic reaction, so being conducive to the hair of hydrolysis with the raising of solution temperature
It is raw, so that, hydrolysis degree increase mobile to hydrolysis direction is balanced, the alkalinity enhancing of solution, while the carbon acid molar ratio generated
It is less, carbon dioxide cannot be decomposed to give off.To which the compound of the flavonoids in filter residue can more be dissolved in liquid system
In, finally make the yield of flavone compound higher；
3, benzene sulfonic acid belongs to the middle strong acid of organic acid, enables to using the pH value in micro i.e. changeable system, from
And the process of adjusting is accelerated, on the other hand, this flavine belongs to organic acid, unstable so as to avoid flavone compound from generating
Fixed oxonium salt increases the yield of flavone compound.
Detailed description of the invention
Fig. 1 is a kind of process flow chart of the extraction process of white pond lily flower extract of the present invention.
Specific embodiment
With reference to the accompanying drawings and examples, present invention is further described in detail.
Embodiment 1:
As shown in Figure 1, a kind of extraction process of white pond lily flower extract, includes the following steps:
S1: pre-treatment.First the petal of the white pond lily to picking back is cleaned, is air-dried, and is ground.
S2: primary to decoct.The petal of white pond lily after pre-treatment is placed in three-necked flask, then in three-necked flask
2 broken tiles are put, deionized water is then added, is decocted, the mass ratio of deionized water and petal is 5:1, decocts 5min.
S3: alkaline environment is adjusted.Sodium ethoxide is added in towards the three-necked flask to boil in S2, so that the liquid in three-necked flask
PH is 8, then proceedes to boil, and continues 20min.
S4: first to filter.Above-mentioned S3 is boiled and continues the liquid after 30min and is taken out while hot using Suction filtration device
Filter, takes filtrate, spare labeled as A filtrate.
S5: weak acid environment is adjusted.By S4, treated that A filtrate moves in new three-necked flask, and heats so that three mouthfuls
The temperature of A filtrate in flask is 60 DEG C, then towards benzene sulfonic acid is added in three-necked flask, adjusts the liquid in three-necked flask
PH to 5 stops addition benzene sulfonic acid.It is followed by stirring for uniformly, standing 8h.
S6: last filters.Last time suction filtration is carried out to S5 treated liquid, is then adequately washed filter residue, directly
It is in neutrality to the surface of filter residue.
S7: dry.Then S6 is washed neutral filter residue to be dried, so that the moisture on filter residue surface is gone, to obtain
The crude product of flavone compound.
Embodiment 2:
A kind of extraction process of white pond lily flower extract, includes the following steps:
S1: pre-treatment.First the petal of the white pond lily to picking back is cleaned, is air-dried, and is ground.
S2: primary to decoct.The petal of white pond lily after pre-treatment is placed in three-necked flask, then in three-necked flask
2 broken tiles are put, deionized water is then added, is decocted, the mass ratio of deionized water and petal is 7:1, decocts 5min.
S3: alkaline environment is adjusted.Sodium ethoxide is added in towards the three-necked flask to boil in S2, and makes the liquid in three-necked flask
It when the pH of body is 10, then proceedes to boil, continues 40min.
S4: first to filter.Above-mentioned S3 is boiled and continues the liquid after 30min and is taken out while hot using Suction filtration device
Filter, takes filtrate, spare labeled as A filtrate.Then filter residue is put into new three-necked flask, then puts 2 broken tiles, then adds
Deionized water is added, and the mass ratio of deionized quality and filter residue is 3:1.After deionized water is added, to three-necked flask
It is heated, so that the liquid boiling in three-necked flask, and lasting decoction 25min, it then while hot will be in three-necked flask
Liquid is filtered, and filtrate is taken, and is labeled as B filtrate.Then A filtrate is uniformly mixed with B filtrate, is labeled as C filtrate.
S5: weak acid environment is adjusted.By S4, treated that C filtrate moves in new three-necked flask, and heats so that three mouthfuls
The temperature of C filtrate in flask is 80 DEG C, then towards adding benzene sulfonic acid in three-necked flask, so that the liquid in three-necked flask
When pH is 4, stop addition benzene sulfonic acid.It is followed by stirring for uniformly, standing 10h.
S6: last filters.Last time suction filtration is carried out to S5 treated liquid, is then adequately washed filter residue, directly
It is in neutrality to the surface of filter residue.
S7: dry.Then S6 is washed neutral filter residue to be dried, so that the moisture on filter residue surface removes.After dried,
Filter residue is recrystallized using boiling water, then filter residue is placed in beaker, then beaker is placed in water-bath in beaker
Filter residue be dried, and the temperature in beaker is made to remain 90 DEG C, the filter residue after recrystallization obtains after being completely dried
The crude product of flavone compound.
Embodiment 3:
A kind of extraction process of white pond lily flower extract, includes the following steps:
S1: pre-treatment.First the petal of the white pond lily to picking back is cleaned, is air-dried, and is ground.
S2: primary to decoct.The petal of white pond lily after pre-treatment is placed in three-necked flask, then in three-necked flask
2 broken tiles are put, deionized water is then added, is decocted, the mass ratio of deionized water and petal is 6:1, decocts 5min.
S3: alkaline environment is adjusted.Sodium ethoxide is added in towards the three-necked flask to boil in S2, and makes the liquid in three-necked flask
It when the pH of body is 9, then proceedes to boil, continues 30min.
S4: first to filter.Above-mentioned S3 is boiled and continues the liquid after 30min and is taken out while hot using Suction filtration device
Filter, takes filtrate, spare labeled as A filtrate.Filter residue is put into new three-necked flask, then places 2 broken tiles, and then addition is gone
Ionized water, and the mass ratio of the quality of deionized water and filter residue is 4:1.Deionized water adds three-necked flask after having added
Heat, so that the liquid boiling in three-necked flask.After liquid boiling in three-necked flask, towards three-necked flask in add carbon
Sour sodium, the addition quality of sodium carbonate and the mass ratio of filter residue are 3:2；Then secondary decoction is carried out, after decocting 25min, is made at once
It is filtered while hot with Suction filtration device, takes filtrate, be labeled as D filtrate.D filtrate mutually mixes with A filtrate, is labeled as mixed filtrate
It is spare for E filtrate.
S5: weak acid environment is adjusted.By S4, treated that E filtrate moves in new three-necked flask, and heats so that three mouthfuls
The temperature of E filtrate in flask is 70 DEG C, then towards adding benzene sulfonic acid in three-necked flask, so that the liquid in three-necked flask
When pH is 4, stop addition benzene sulfonic acid.It is followed by stirring for uniformly, standing 9h.
S6: last filters.Last time suction filtration is carried out to S5 treated liquid, is then adequately washed filter residue, directly
It is in neutrality to the surface of filter residue.
S7: dry.Then S6 is washed neutral filter residue to be dried, so that the moisture on filter residue surface removes.After dried,
Filter residue is recrystallized using boiling water, then filter residue is placed in beaker, then beaker is placed in water-bath in beaker
Filter residue be dried, and the temperature in beaker is made to remain 75 DEG C, the filter residue after recrystallization obtains after being completely dried
The crude product of flavone compound.
Comparative example 1:
Regulator used in the step of difference with embodiment 3 is, S3 tune alkaline environment is sodium hydroxide.
Comparative example 2:
The pH in concentrated hydrochloric acid regulation system is used in the step of difference with embodiment 3 is, S5 tune weak acid environment.
Test:
The petal of picking 50g white pond lily is divided into 5 parts after pre-treatment, uses embodiment 1-3 and right respectively
The crude product of the extraction flavone compound of extraction process documented by ratio 1-2.Finally obtained flavone compound is claimed
Amount and the content detection that flavone compound is carried out using HPLC.As a result as shown in the table:
Data analysis:
In conjunction with upper table, it is not difficult to find out that, using extraction process documented by embodiment 3 in the present invention, the flavones that finally obtains
The crude product of class compound is most, and in crude product contained flavonoid content highest, finally make flavone compound
Yield highest.
The quality of the crude product of the flavone compound extracted using extraction process documented by embodiment 1 relative to
It is minimum in tri- samples of embodiment 1-3, it is because having one with regard to directly carrying out adjusting weak acid environment after first filter
The crude product of part flavone compound is discarded with the filter residue after first filter, to increase the waste of resource.Meanwhile
In embodiment 1 it is last it is dried after, boiling water recrystallization is not carried out, so that contained flavone compound in last crude product
Percentage composition is lower.
The quality of the crude product of the flavone compound extracted using extraction process documented by embodiment 2 relative to
It is in by-level in tri- samples of embodiment 1-3, is because having carried out decoction again to filter residue after first filter, to the greatest extent
Pipe be filter residue is handled again, but due to be only merely decoct, without using sodium carbonate, cause only some
The crude product of flavone compound is extracted from filter residue.But finally, due to the thick of the flavone compound after drying
Product carry out boiling water recrystallization, so that the content of flavone compound contained in last product containing than embodiment 1
Amount is high.
The quality of the crude product of the flavone compound extracted using documented extraction process in comparative example 1 is minimum,
Be because the regulator used in the step of adjusting alkaline environment be sodium hydroxide, can not be by the flavonoids of deeper inside in petal
Compound extracts, and the quality for eventually leading to the crude product of flavone compound is minimum.
The crude product of the flavone compound extracted using documented extraction process in comparative example 2 it is of low quality,
It is because being adjusted during adjusting weak acid environment using concentrated hydrochloric acid, although it is also possible that the pH value of system is in acid
Property, but concentrated hydrochloric acid belongs to inorganic acid, easily generates unstable oxonium salt with flavone compound and dissolves in a liquid, thus
So that the crude product filter residue of last flavone compound is reduced, the final yield for influencing flavone compound.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of extraction process of white pond lily flower extract, characterized by the following steps:
S1: pre-treatment is carried out to the petal of white pond lily；
S2: primary to decoct；Deionized water is added to decoct on the petal after pre-treatment, and the mass ratio for adding deionized water and petal is
(5-7):1；
S3: alkaline environment is adjusted；To regulator is added in the liquid after S2 boiling, so that the pH of system is adjusted to 8-10, and herein
System is made to continue the 20-40min that boils under conditions of pH；
S4: first to filter；To S3, treated that liquid filters at once, takes filtrate, and be labeled as A filtrate；
S5: weak acid environment is adjusted；After A filtrate is heated to 60-80 DEG C, the pH of A filtrate is adjusted to 4-5, is then stirred evenly,
And stand 8-10h；
S6: last filters；Last time suction filtration is carried out to S5 treated liquid, and filter residue is washed till neutrality；
S7: dry；S6 is washed till to neutral filter residue and drying；
The crude product of flavone compound is arrived after S7.
2. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: the S3 alkali tune
Added regulator is sodium ethoxide in environment.
3. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: the S4 takes out for the first time
After filter processing, adds deionized water to decoct 20-30min again the filter residue of this step, and filtered at once, take filtrate, mark
For B filtrate；Then A filtrate is uniformly mixed with B filtrate, is labeled as C filtrate.
4. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: the S4 takes out for the first time
After filter processing, the filter residue of this step is added into deionized water and is heated to boiling, then adds sodium carbonate, then carries out secondary decoct
It boils, after decocting 20-30min, is filtered again at once, take filtrate, be labeled as D filtrate；Then A filtrate is mixed mutually with D filtrate
It closes, is labeled as E filtrate.
5. a kind of extraction process of white pond lily flower extract according to claim 3 or 4, it is characterised in that: S4 takes out for the first time
Void is towards deionized water is added in filter residue after filter, and the mass ratio of quality added by deionized water and filter residue is (3-5): 1.
6. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: the S2 is once decocted
Before boiling, 1-2 broken tiles are added in the system for needing to decoct.
7. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: the S5 tune weak acid
The pH of A filtrate is adjusted to 4-5 using benzene sulfonic acid in property environment.
8. a kind of extraction process of white pond lily flower extract according to claim 1, it is characterised in that: after S7 drying
Filter residue is recrystallized using boiling water, and is dried at 60-90 DEG C, and the crude product of flavone compound is obtained.
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| CN113209008A (en) * | 2021-05-27 | 2021-08-06 | 新疆维吾尔自治区药物研究所 | Water lily polyphenol gel and preparation method and application thereof |
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|---|---|---|---|---|
| CN104926766A (en) * | 2014-03-17 | 2015-09-23 | 湖北博瑞生物科技有限公司 | Method for synchronous extraction of quercetin and nuciferine from lotus leaves |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104926766A (en) * | 2014-03-17 | 2015-09-23 | 湖北博瑞生物科技有限公司 | Method for synchronous extraction of quercetin and nuciferine from lotus leaves |
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| CN113209008A (en) * | 2021-05-27 | 2021-08-06 | 新疆维吾尔自治区药物研究所 | Water lily polyphenol gel and preparation method and application thereof |
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