CN109939064A - The Essence of the metabolite containing probiotics fermention and its application in cosmetics - Google Patents
The Essence of the metabolite containing probiotics fermention and its application in cosmetics Download PDFInfo
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Abstract
The invention belongs to skin care fields, a kind of method for specifically disclosing the cosmetics Essence containing probiotics fermention metabolite and preparing the Essence, and it is somebody's turn to do application of the Essence containing probiotics fermention metabolite in cosmetics, the cosmetics Essence includes the active probiotic metabolite solution that mass fraction is 10%-70%, and surplus is basic liquid；The active probiotic metabolite solution is that the nutrient solution containing loose tea extract and extract from fruit is obtained through probiotics fermention, wherein, the pine tea extract is the aqueous extract of fresh pine needle and green tea, nutrient solution can will be not easy to be converted to easy absorbed substance by the nutritional ingredient of percutaneous absorption in pine needle, green tea and fruit through probiotics fermention, also add unexistent functional activity substance in simple probiotics fermention metabolite, the problem of Essence can improve dark and gloomy skin, desiccation, wrinkle is used for a long time, skin is made to keep tender white.
Description
Technical field
The invention belongs to skin care field more particularly to a kind of Essence containing probiotics fermention metabolite and preparations
The application of the method for the Essence and the Essence in cosmetics.
Background technique
The structure of skin includes epidermis, skin corium and subcutaneous fat.Skin corium include collagen, elastin and its
His fibr tissue.People are for a long time against computer and mobile phone electronic equipment, electronic equipment attraction dust or small easy to produce static electricity
Particle is attached to skin surface, irregular along with working and resting, and epidermis is easily dry, exacerbates melanin deposition.With the increasing at age
Long, epidermis is gradually thinning, and skin metabolism slows down, and the moisturizing factor in skin corium is reduced, in skin corium elastic fibers and
Collagenous fibres hypofunction, elastic force weaken and then occur wrinkle；And the intracorporal active biological enzyme of people is reduced, a large amount of free radical
Human body cell is destroyed, the process of skin aging is accelerated.
There are the micropopulations such as a large amount of microorganism, including bacterium, fungi and virus for normal skin surface, are formed
Mutually restrict the micropopulation of balance.Under normal circumstances, micropopulation can be present in skin surface in an orderly manner, can not only protect
Hold the stabilization of skin surface micro-ecological environment, additionally it is possible to which secretion is produced as bioactive substance used in skin.
Bacillus acidi lactici is a kind of microorganism for surviving in the health that is beneficial to man in enteron aisle, can improve the micro- of host
The ecological balance forms antibacterial biological barrier, inhibits the growth and breeding of sex pheromone and spoilage organisms, plays the work of inhibiting bacteria and diminishing inflammation
With.Importantly, the metabolite that lactobacillus fermentation generates has significant role to skin is improved, these metabolites include
Organic acid, amino acid, vitamin, exocellular polysaccharide, small-molecule peptide and biological enzyme, organic acid can balance skin surface pH, make
The pH of skin is weakly acidic, and amino acid, micro-element and biological enzyme can repair epidermal cell, removes pigementation, improves
Pachylosis makes skin Lightening and high resilience；Microbial exopolysaccharides can also anti-oxidant delay skin aging.
As Chinese patent CN108478459 discloses a kind of eye essence of solution of lysis containing lactobacillus fermentation, the eye
Portion's essence is using the oxidation resistant body frame of anti-aging of tea extract, pomegranate rind extract, caffeine three as eye essence
Frame improves the absorbability of skin in conjunction with lactobacillus fermentation lysate, while utilizing nutritional ingredient royal jelly polypeptide, swallow
Wheat beta glucan, buckwheat seed extract and ceramide are that skin supplements nutrient and moisture, can be used for solving eye skin aging
The problem of.
In addition, the metabolin of Bacillus acidi lactici also includes some small molecule antibacterials and antioxidant, these substances can press down
The breeding of skin surface harmful bacteria processed has effects that good anti-acne antiplaque.Chinese patent CN109498543 is disclosed for adjusting
The composition of section skin microecological balance is made of white ginseng fungus extract, probiotics metabolite and pearl shellfish meat anti-oxidation peptide, benefit
Inhibit the growth of harmful bacteria with a large amount of acidic materials in probiotics metabolite, while probiotics metabolite also has powder
Pierce the effect of cutin, shining skin skin lightening.
Currently, the skin care item of the metabolite containing profitable probliotics are numerous in the market, but the effect of most commodity only stops
In publicity level.Be also added into natural extract in some commodity, as also added in CN108478459 tea extract and
Pomegranate rind extract, however these commodity have also only carried out probiotics metabolite solution and other extracts simply
Mixing, is difficult to make full use of the characteristic of each component and element in these natural extracts and probiotics metabolite.Therefore it provides
A kind of whitening effect is obvious, is even more important using the excellent skin care substance of safe, performance of keeping humidity.
Summary of the invention
It is an object of the invention to combine the deficiencies in the prior art, a kind of cosmetics Essence is provided, in the Essence
Metabolite containing probiotics fermention pine tea extract and fermenting fruit extracting solution, repair epidermal cell, tender white muscle skin,
There is remarkable result in terms of skin moisturizing and removing acne and freckle.
Another object of the present invention is to provide the methods for preparing above-mentioned Essence, and the preparation method is easy to operate, easily
In control, and high production efficiency, it is at low cost, it can be used in being mass produced.
The purpose of the present invention is achieved through the following technical solutions:
A kind of cosmetics Essence of the metabolite containing probiotics fermention, the Essence include that mass fraction is 10%-
70% active probiotic metabolite solution, surplus are basic liquid；The active probiotic metabolite solution is probiotics
Fermented nutritive liquid obtains, and the nutrient solution includes loose tea extract and extract from fruit, wherein the pine tea extract is fresh pine
The aqueous extract of needle and green tea.
Preferably, the active probiotic metabolite solution preparation method comprising steps of
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle and green tea, the mixture of fresh pine needle and green tea is added into boiling
Boiling, is obtained by filtration loose tea extract while hot, and appropriate fruit is added in loose tea extract, and mixing juicing obtains the nutrient solution；
(2) it prepares the culture medium containing loose tea: the mixed of glucose, yeast powder and potassium dihydrogen phosphate being added in Xiang Suoshu nutrient solution
Liquid is closed, is sterilized after mixing evenly, it is cooling stand-by；
(3) active probiotic metabolite solution is prepared: the access mixing probiotics bacterial on the culture medium containing loose tea
Kind, mix static fermentation, pH is down to 3.6 or less and the content of superoxide dismutase when reaching setting value, and taking-up is centrifuged
Processing, filtering clarification, obtains the probiotics fermention metabolite solution containing active material.
In above-mentioned preparation method, the mass ratio of fruit and fresh pine needle is 4-20 in step (1).Pine is extracted using water extract method
Active principle in needle and green tea, can effectively save the substances such as polyphenol, amino acid and the microorganism in pine needle and green tea, wherein water
Mass ratio with fresh pine needle is 15-100:1, and the time for adding boiling to boil is preferably 10-30min.Probiotics in the embodiment of the present invention
The temperature of fermentation is 30 DEG C -40 DEG C, and preferable temperature is 30 DEG C -37 DEG C.The mass fraction of each component in mixed liquor are as follows: glucose
1.0%-15.0%, yeast powder 0.1-5.0%, potassium dihydrogen phosphate 0.01-1.0%, surplus are the nutrient solution.Probiotics fermention
When detection pH variation and bioactive substance superoxide dismutase (SOD), if pH reduce it is more, illustrate fermentation complete.
At above-mentioned temperature, pH is down to 3.6 or less and the content of superoxide dismutase when reaching setting value, and fermentation time is preferably 6-24
Hour.
Polyphenol compound is the mixture for having one or more aromatic rings and containing one or several hydroxyl groups, polyphenol
Contain a large amount of phenolic hydroxyl group in molecule, donor of the phenolic hydroxyl group as hydrogen can capture the free radical generated in oxidation process, inhibit
Or oxidation process is interrupted, balance the quantity of base interior free yl.And contain relatively rich phenolic compound in pinaceae plant, pine needle
In not only polyphenol content it is high, also contain more flavones and procyanidine, be one of antioxidant important in pine needle.Only make
It is fermented with pine needle extract and tea extract, rather than is filtered after pine needle and green tea slag are fermented together, be able to solve pine needle
Make to be metabolized solution during the fermentation in steel gray problem.
Contain the substances such as tea polyphenols, caffeine, minerals, amino acid and microorganism in green tea.Tea polyphenols are the masters of green tea
Ingredient is wanted, active hydrogen can be provided to remove free radical, inhibit melanin to be formed, mitigate pigment deposition.
Active bio ingredient rich in extract from fruit, wherein tartaric acid can accelerate cell turnover speed and promotion
Dead cell is detached from, and can make skin-whitening, moisturizing, anti-wrinkle, also have the function of anti-aging；Vitamin C and its derivative can
The intermediate of melanin is restored to inhibit the formation of melanin, moreover it is possible to restore dark oxidized form melanin.
Above-mentioned fruit can be in orange, apple, mango, fresh dates, Kiwi berry, hawthorn, grape, strawberry, premium, tomato
It is one or more.
Preferably, the fruit is the orange and apple of equal proportion.Apple is rich in carbohydrate, vitamin C, vitamin E, inorganic
Salt, vitamin, organic acid, polyphenol and flavone compound, in transparent apple containing more catechin, procyanidine and
Chlorogenic acid, can anti-oxidant, anti-aging, in addition, also containing quenching singlet oxygen and peroxylradicals can be removed in apple
Beta carotene.
Relatively rich phenolic compound is remained in the loose tea extract that pine needle and green tea directly add boiling to boil, it will be upper
Stating culture medium made of loose tea extract and extract from fruit can obtain after probiotics fermention containing the molten of active material
Liquid, vitamin, amino acid, enzyme, minerals, polypeptide, secondary metabolite and biodiasmin rich in etc., probiotics make
For fruit and loose tea cell, the ingredient that can be used for improving skin can be sufficiently discharged and generate, in probiotics metabolite
On nutrition foundation, the functional activities object such as amino acid, minerals, vitamin, Polyphenols, flavonoids and anthocyan is increased
Matter.
Preferably, above-mentioned mixing probiotics include equal proportion lactobacillus plantarum (Lactobacillus plantarum),
Lactobacillus acidophilus (Lactobacillus acidlophilus), Lactobacillus casei (Lactobacillus casei), rhamnose
Lactobacillus (Lactobacillus.rhamnosus) strain.
Preferably, the mass ratio of the fresh pine needle and the green tea is 5-15:1.
Preferably, mass fraction of the component of the basal liquid in the cosmetics Essence is formed are as follows: moisturizer
2.2%-35%, anti-sensitizer 0.1%-3%, thickener 0.1%-3.0%, emulsifier 0.1%-3%, preservative 0.1%-1%,
Allantoin 0.1%-5%, biological polypeptide 0.1%-5.0%, sterile water to 100%.
Preferably, the moisturizer includes each component that following mass percent is accounted in the cosmetics Essence: DPG
0.5%-5%, -26 0.1%-5% of glycerin polyether, polyglutamic acid sodium 0.05%-2.0%, hydroxyethyl cellulose 0.05-
2.0%, HA 0.1-3.0%, D-pantothenyl aleohol 0.1-2.0%, glycine betaine 0.1-3.0%, ERT 37 0.1-3.0%, MultiEX
Waterin 1.0-5.0%, starlub-7 0.1%-5%.
Preferably, the anti-sensitizer selects Chinese herbal medicine extract.Preferably centella, polygonum cuspidate, radix scutellariae, green tea, light fruit are sweet
Seven Plant Extracts such as grass, german chamomile, rosemary.
Preferably, the emulsifier is water-soluble silicone wax compound.The water-soluble silicone wax compound is specially wax-like gathers
Ether modified dimethyl polysiloxane copolymer.
Preferably, thickener is transparent xanthan gum.
Preferably, preservative selects composite, can be parahydroxyacet-ophenone, 91 preservative of A, each component additive amount
For parahydroxyacet-ophenone 0.1%-1.0%, A 910.1%-1.0%.
Biological polypeptide is preferably Argireline.
The present invention also provides a kind of preparation methods of the Essence of above-mentioned metabolite containing probiotics fermention, including step
It is rapid:
(1) thickener, emulsifier are mixed with sterile water, emulsifying stirs at low speed system, then carries out at defoaming
Reason；
(2) after temperature is down to preset temperature, be added the probiotics metabolite solution containing active material, moisturizer,
Anti-sensitizer, thickener, preservative, allantoin, biological polypeptide are stirred at low speed and are produced up to the metabolism containing probiotics fermention
The Essence of object.
The present invention also provides it is a kind of include the above-mentioned Essence of metabolite containing probiotics fermention facial mask.Contain above-mentioned
To get the facial mask containing active probiotic metabolite in the Essence injection mask substrate bag of probiotics fermention metabolite.
The present invention also provides application of the Essence of the above-mentioned metabolite containing probiotics fermention in cosmetics.This is contained
When the Essence of probiotics fermention metabolite is used for different types of cosmetics, the type and content of basal liquid each component can be made
Corresponding adjustment, as be used for lotion, milky lotion, frost, facial treatment essence, facial mask type product in, preferred facial mask.
The beneficial effects of the present invention are: the Essence of the invention containing active probiotic metabolite is anti-oxidant, goes
Free radical ability is strong, by probiotics fermention, will be not easy to be converted to by the nutritional ingredient of percutaneous absorption in pine needle, green tea and fruit
Easy absorbed substance, such as glycerol, lactic acid, lipid, also add unexistent in simple probiotics fermention metabolite
The functional activities substance such as flavonoids and anthocyan, improves the content of polyphenols.Functional activity object in Essence
Matter can accelerate cell turnover speed, accelerate to remove free radical, to repair epidermal cell, for dermal cell supplement moisturizing because
Son has the function that the tender white muscle skin of moisturizing and skin is made to keep elastic force.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
Raw material in the present invention specifically:
Biologically active polypeptide is preferably Argireline；Starlub-7 is PEG/PPG-17/6 copolymer, is purchased from South Korea
Bio Spectrum company；MultiEX Waterin is carbohydrate isomer, is purchased from South Korea Bio Spectrum company；Water
Dissolubility silicon wax compound is specially wax-like polyether-modified polydimethylsiloxanecopolymer copolymer, trade name DMC 6038；Antiallergic
Chinese herbal medicine extract, preferably seven kinds of plants such as centella, polygonum cuspidate, radix scutellariae, green tea, glycyrrhiza glabra, german chamomile, rosemary are selected in agent
Extract is purchased from South Korea Bio Spectrum company, trade name CalmYang.
Embodiment 1
Prepare the Essence A of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 5.0g and green tea 1.0g, be added in 500mL water, boil
10min is made pine tea liquid, then fresh orange 50.0g, apple 50.0g is added in loose tea liquid and carry out mixing juicing；
(2) it prepares the culture medium containing loose tea: in loose tea orange fruit mixed nutrient solution, adding the group of following mass fraction
Point: glucose 1.0%, yeast powder 0.1%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) it prepares active probiotic metabolite solution: in culture medium after sterilization, then accessing and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification；
(4) prepare the Essence containing active material: each component of following mass fraction is added: probiotics fermention metabolism produces
Basal liquid: DPG 0.2%, object solution 55.0% Starlub-7 0.2%, glycerin polyether -26 0.2%, A 91 0.1%, gathers
Sodium glutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA0.5%, parahydroxyacet-ophenone 0.1%,
D-pantothenyl aleohol 0.2%, glycine betaine 0.2%, ERT37 0.1%, allantoin 0.2%, MultiEX Waterin 1.5%,
CalmYang 0.1%, Argireline 0.3%, DMC 6,038 0.3%, with sterile water 100% is complemented to, by above-mentioned object
The first thoroughly dissolution, emulsification in water of matter, is uniformly mixed, stable emulsion is made to get the Essence containing active material is arrived.
Embodiment 2
Prepare the Essence B of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 10.0g and green tea 2.0g, be added in 500mL water, boil
10min is made pine tea liquid, then fresh orange 50.0g, apple 50.0g is added in loose tea liquid and carry out mixing juicing；
(2) it prepares the culture medium containing loose tea: in loose tea orange fruit mixed nutrient solution, adding the group of following mass fraction
Point: glucose 1.5%, yeast powder 0.2%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) it prepares active probiotic metabolite solution: in culture medium after sterilization, then accessing and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification；
(4) prepare the Essence containing active material: each component of following mass fraction is added: probiotics fermention metabolism produces
Object solution 50.0%, basal liquid: DPG 0.1%, Starlub-7 0.%, glycerin polyether -260.2%, A 91 0.1%, poly- paddy
Propylhomoserin sodium 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, parahydroxyacet-ophenone 0.2%, D-
Panthenol 0.2%, glycine betaine 0.2%, ERT37 0.2%, allantoin 0.5%, MultiEX Waterin 1.0%, CalmYang
0.1%, Argireline 0.2%, DMC 6,038 0.2%, with sterile water complement to 100%, by above-mentioned substance first in water
In thoroughly dissolve, emulsify, be uniformly mixed, stable emulsion be made to get to the Essence containing active material.
Embodiment 3
Prepare the Essence C of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 20.0g and green tea 2.0g, be added in 500mL water, boil
15min is made pine tea liquid, then fresh orange 60.0g, apple 60.0g is added in loose tea liquid and carry out mixing juicing；
(2) it prepares the culture medium containing loose tea: in loose tea orange fruit mixed nutrient solution, adding the group of following mass fraction
Point: glucose 1.2%, yeast powder 0.5%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) it prepares active probiotic metabolite solution: in culture medium after sterilization, then accessing and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification；
(4) prepare the Essence containing active material: each component of following mass fraction is added: probiotics fermention metabolism produces
Basal liquid: DPG 0.3%, object solution 40.0% Starlub-7 0.3%, glycerin polyether -26 0.2%, A 91 0.1%, gathers
Sodium glutamate 0.1%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.3%, parahydroxyacet-ophenone 0.3%,
D-pantothenyl aleohol 0.3%, glycine betaine 0.3%, ERT 37 0.2%, allantoin 1.0%, MultiEX Waterin 1.0%,
CalmYang 0.1%, Argireline 1.5%, DMC 6,038 0.5%, with sterile water 100% is complemented to, by above-mentioned object
The first thoroughly dissolution, emulsification in water of matter, is uniformly mixed, stable emulsion is made to get the Essence containing active material is arrived.
Embodiment 4
Prepare the Essence D of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 25.0g and green tea 3.0g, be added in 500mL water, boil
15min is made pine tea liquid, then fresh orange 60.0g, apple 60.0g is added in loose tea liquid and carry out mixing juicing；
(2) it prepares the culture medium containing loose tea: in loose tea orange fruit mixed nutrient solution, adding the group of following mass fraction
Point: glucose 1.3%, yeast powder 0.5%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) it prepares active probiotic metabolite solution: in culture medium after sterilization, then accessing and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification；
(4) prepare the Essence containing active material: each component of following mass fraction is added: probiotics fermention metabolism produces
Basal liquid: DPG 0.5%, object solution 35.0% Starlub-7 0.2%, glycerin polyether -26 0.3%, A 91 0.2%, gathers
Sodium glutamate 0.1%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, parahydroxyacet-ophenone 0.2%,
D-pantothenyl aleohol 0.3%, glycine betaine 0.3%, ERT 37 0.2%, allantoin 1.5%, MultiEX Waterin 1.2%,
CalmYang 0.1%, Argireline 1.5%, DMC 6,038 0.5%, with sterile water 100% is complemented to, by above-mentioned object
The first thoroughly dissolution, emulsification in water of matter, is uniformly mixed, stable emulsion is made to get the Essence containing active material is arrived.
Embodiment 5
Prepare the Essence E of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 30.0g and green tea 2.5g, be added in 500mL water, boil
10min is made pine tea liquid, then fresh orange 100.0g, apple 100.0g is added in loose tea liquid and carry out mixing juicing；
(2) it prepares the culture medium containing loose tea: in loose tea orange fruit mixed nutrient solution, adding the group of following mass fraction
Point: glucose 0.5%, yeast powder 0.1%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) it prepares active probiotic metabolite solution: in culture medium after sterilization, then accessing and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification；
(4) prepare the Essence containing active material: each component of following mass fraction is added: probiotics fermention metabolism produces
Object solution 60.0%, basal liquid: DPG 0.25%, Starlub-7 0.25%, glycerin polyether -26 0.15%, A 91
0.15%, polyglutamic acid sodium 0.15%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, para hydroxybenzene
Ethyl ketone 0.25%, D-pantothenyl aleohol 0.35%, glycine betaine 0.35%, ERT 370.25%, allantoin 2.5%, MultiEX Waterin
1.5%, CalmYang 0.15%, Argireline 1.5%, DMC 6,038 0.5%, with sterile water complement to 100%,
By above-mentioned substance, first thoroughly dissolution, emulsification in water, is uniformly mixed, stable emulsion is made to get to containing active material
Essence.
Comparative example 1
Prepare Essence F: itself the difference from embodiment 1 is that, fresh pine needle is not added in nutrient solution, by green tea extractive liquor with
Fruit squeezes the juice to obtain nutrient solution.
(1) it prepares nutrient solution: weighing green tea 1.0g, be added in 500mL water, boil 10min, obtain green tea extractive liquor,
Fresh orange 50.0g, apple 50.0g are added in green tea extractive liquor again and carry out mixing juicing；
(2) it prepares culture medium: the component of following mass fraction: glucose 1.0%, yeast powder being added in nutrient solution
0.1%, potassium dihydrogen phosphate 0.01%, 105 DEG C of sterilizing 10min are cooled to 35 DEG C, spare；
(3) probiotics metabolite solution is prepared: in culture medium after sterilization, then mature by equal proportion access culture
Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours, filtering
Clarification；
(4) it prepares Essence: each component of following mass fraction: probiotics fermention metabolite solution 55.0% is added,
Basal liquid: DPG 0.2%, Starlub-7 0.2%, glycerin polyether -26 0.2%, A 91 0.1%, polyglutamic acid sodium
0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA0.5%, parahydroxyacet-ophenone 0.1%, D-pantothenyl aleohol
0.2%, glycine betaine 0.2%, ERT37 0.1%, allantoin 0.2%, MultiEX Waterin 1.5%, CalmYang
0.1%, Argireline 0.3%, DMC 6,038 0.3%, with sterile water complement to 100%, by above-mentioned substance first in water
In thoroughly dissolve, emulsify, be uniformly mixed, stable emulsion be made to get to the Essence containing active material.
Comparative example 2
Prepare Essence G: itself the difference from embodiment 1 is that, the nutrient solution containing loose tea without probiotics fermention processing,
Probiotics fermention metabolite mixes molten as the probiotics fermention metabolite containing loose tea extract with the nutrient solution containing loose tea
Liquid.
Prepare the Essence A of the metabolite containing probiotics fermention:
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle 5.0g and green tea 1.0g, be added in 500mL water, boil
10min is made pine tea liquid, then fresh orange 50.0g, apple 50.0g is added in loose tea liquid and carry out mixing juicing；
(2) culture medium is prepared by the component of following mass fraction: glucose 1.0%, yeast powder 0.1%, potassium dihydrogen phosphate
0.01%, 105 DEG C of sterilizing 10min, are cooled to 35 DEG C, spare；In culture medium after sterilization, then accesses and be trained by equal proportion
Ripe lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus strain, 35 DEG C of static fermentations are fermented 8 hours,
Filtering clarification, probiotics fermention metabolite is mixed with the nutrient solution containing loose tea, obtains the probiotics hair containing loose tea extract
Ferment metabolite；
(3) prepare Essence: each component of following mass fraction is added: the probiotics fermention metabolism containing loose tea extract produces
Basal liquid: DPG 0.2%, object solution 55.0% Starlub-7 0.2%, glycerin polyether -26 0.2%, A 91 0.1%, gathers
Sodium glutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA0.5%, parahydroxyacet-ophenone 0.1%,
D-pantothenyl aleohol 0.2%, glycine betaine 0.2%, ERT37 0.1%, allantoin 0.2%, MultiEX Waterin 1.5%,
CalmYang 0.1%, Argireline 0.3%, DMC 6,038 0.3%, with sterile water 100% is complemented to, by above-mentioned object
The first thoroughly dissolution, emulsification in water of matter, is uniformly mixed, stable emulsion is made to get the Essence containing active material is arrived.
Embodiment 1- embodiment 5 and the Essence A-E being prepared are injected into mask substrate bag to get live probiotic is contained
The facial mask of bacterium metabolite.The cosmetics Essence F-G of comparative example 1-2 is prepared into facial mask by same procedure processing.
Skin care test
Experimental group: blee dimness patient 30, more wrinkle patient 30 of face are randomly divided into 3 groups, i.e., every group contains
10 blee dimness patients and the more wrinkle patients of 10 faces.Use the face of corresponding embodiment 1, embodiment 3 and embodiment 5
Film.Daily using once, being used continuously 4 weeks, test uses 1 day, 3 days, 7 days, 14 days, 21 days and 28 days skin respectively
(skin performance can specifically be examined by skin brightness, skin elasticity value and dermatoglyph characterization by VISIA skin detection instrument
It surveys).
Control group:
Skin care test is carried out with the facial mask that comparative example 1-2 is prepared, is denoted as comparative example 1 and comparative example 2；With embodiment 5
Basal liquid in preparation process is denoted as comparative example 3 as control.
Every group of comparative example corresponds to blee dimness patient 10, more wrinkle patient 10 of face, is used continuously 4 weeks, point
1 day, 3 days, 7 days, 14 days, 21 days and 28 days skin Ce Shi not used.
Above-described embodiment and the result of comparative example test take the mean value of the test result of every group of patient.Test result is as follows
Shown in table:
1 experimental group skin care test result of table
2 control group skin care test result of table
According to comparative analysis before and after test result and use, face skin dimness person has obtained apparent improvement, and skin is white
Degree improves obviously, and the elasticity and moisture of skin are also significantly improved, and wrinkle desalination is obvious.Long-time service can improve skin
The problem of dark and gloomy, desiccation, wrinkle, makes skin keep tender white.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the patents of the invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of cosmetics Essence of metabolite containing probiotics fermention, which is characterized in that the cosmetics Essence includes
Mass fraction is the active probiotic metabolite solution of 10%-70%, and surplus is basic liquid；The active probiotic metabolism produces
Object solution is that probiotics fermention nutrient solution obtains, and the nutrient solution includes loose tea extract and extract from fruit, wherein the pine
Tea extract is the aqueous extract of fresh pine needle and green tea.
2. the cosmetics Essence of the metabolite containing probiotics fermention as described in claim 1, which is characterized in that the activity
The preparation method of probiotics metabolite solution comprising steps of
(1) it prepares the nutrient solution containing loose tea: weighing fresh pine needle and green tea, add boiling to boil in the mixture of fresh pine needle and green tea, take advantage of
Heat filtering obtains loose tea extract, and appropriate fruit is added in loose tea extract, and mixing juicing obtains the nutrient solution；
(2) it prepares the culture medium containing loose tea: the mixing of glucose, yeast powder and potassium dihydrogen phosphate being added in Xiang Suoshu nutrient solution
Liquid sterilizes after mixing evenly, cooling stand-by；
(3) prepare active probiotic metabolite solution: the access mixing probiotics strain on the culture medium containing loose tea mixes
Closing static fermentation, pH is down to 3.6 or less and the content of superoxide dismutase when reaching setting value, and it takes out and carries out centrifugal treating,
Filtering clarification, obtains the probiotics fermention metabolite solution containing active material.
3. the cosmetics Essence of the metabolite containing probiotics fermention as claimed in claim 2, which is characterized in that the fruit
Orange and apple including equal proportion；The mixing probiotics includes lactobacillus plantarum, lactobacillus acidophilus, the cheese cream of equal proportion
Bacillus and Lactobacillus rhamnosus.
4. the cosmetics Essence of the metabolite containing probiotics fermention as claimed in claim 2, which is characterized in that the fresh pine
The mass ratio of needle and the green tea is 5-15:1.
5. the cosmetics Essence of the metabolite containing probiotics fermention as described in claim 1, which is characterized in that described in composition
Mass fraction of the component of basal liquid in the cosmetics Essence are as follows: moisturizer 2.2%-35%, anti-sensitizer 0.1%-
3%, thickener 0.1%-3.0%, emulsifier 0.1%-3%, preservative 0.2%-2%, allantoin 0.1%-5% are biological
Polypeptide 0.1%-5.0%, sterile water to 100%.
6. the cosmetics Essence of the metabolite containing probiotics fermention as claimed in claim 5, which is characterized in that the moisturizing
Agent includes each component that following mass percent is accounted in the cosmetics Essence: DPG0.5%-5%, glycerin polyether-
260.1%-5%, polyglutamic acid sodium 0.05%-2.0%, hydroxyethyl cellulose 0.05-2.0%, HA0.1-3.0%, D-pantothenyl aleohol
0.1-2.0%, glycine betaine 0.1-3.0%, ERT 370.1-3.0%, MultiEX Waterin 1.0-5.0%, starlub-
70.1%-5%.
7. the cosmetics Essence of the metabolite containing probiotics fermention as claimed in claim 5, which is characterized in that the antiallergic
Chinese herbal medicine extract is selected in agent；The emulsifier is water-soluble silicone wax compound.
8. a kind of side for the cosmetics Essence for preparing the metabolite containing probiotics fermention described in claim 1-7 any one
Method, which is characterized in that comprising steps of
(1) thickener, emulsifier are mixed with sterile water, emulsifying stirs at low speed system, then carries out defoaming treatment；
(2) after temperature is down to preset temperature, the probiotics metabolite solution containing active material, moisturizer, antiallergic is added
Agent, thickener, preservative, allantoin, biological polypeptide stir at low speed up to the metabolite containing probiotics fermention
Essence.
9. a kind of facial mask contains the cosmetics of the metabolite containing probiotics fermention as described in claim 1-7 any one
Essence.
10. a kind of cosmetics Essence of the metabolite containing probiotics fermention as described in claim 1-7 any one is being changed
Application in cosmetic, preferably facial mask.
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