CN109929893A - The zymotechnique of low-cost high-quality xanthan gum - Google Patents
The zymotechnique of low-cost high-quality xanthan gum Download PDFInfo
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Abstract
The invention belongs to fermentation technical fields, disclose the zymotechnique of low-cost high-quality xanthan gum, it, which includes the following steps: to access Xanthomonas campestris in the fermentor equipped with fermentation medium, carries out fermented and cultured, fermentor and ceramic membrane are coupled, fermentation time is 48h, by the fermentation liquid in fermentor through ceramic membrane separation, obtain filtrate and concentration thallus, filtrate is drained into feed liquid storage tank, concentration thallus is returned fermentor, fermentation tank culture medium is added into fermentor simultaneously, continue the 36-48h that ferments, obtain fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane separation, obtain filtrate and concentration thallus, filtrate is drained into feed liquid storage tank.The present invention avoids the shortage due to nutrients, the problems in the fermentation such as living environment is deteriorated, feedback inhibition of xanthan gum, improves the total output of xanthan gum in such a way that film is coupled dialysis fermentation.
Description
Technical field
The invention belongs to fermentation technical fields, and in particular to the zymotechnique of low-cost high-quality xanthan gum.
Background technique
Xanthan gum is also known as yellow glue, xanthan gum, and Xanthan Gum is that a kind of monospore generated by pseudo xanthomonas fermentation is more
Sugar, through aerobic fermentation biotechnology, is cut by cabbage black rot xanthomonas campestris using carbohydrate as primary raw material
After opening branch, a kind of acidic extracellular heteroglycan of acidity of straight chain composition is bonded to by Isosorbide-5-Nitrae-for disconnected 1,6- glycosidic bond.Nineteen fifty-two is by the U.S.
The isolated Xanthomonas campestris pv. campestris of the Ministry of Agriculture Illinois Lille Pi Ao the north research institute, and make cabbage extract
It is converted into water-soluble acidic extracellular heteroglycan of acidity and obtains.
Xanthan gum is to be fermented by carbohydrate through Xanthomonas campestris, the more pools of extracellular microorganism of generation.Since its macromolecular is special
Different structure and colloid property, and there are multiple functions, it can be used as emulsifier, stabilizer, gelling thickener, size, film molding
Agent etc., is widely used in all fields of national economy.Xanthan gum is that collection is thickened, suspended, emulsifying, being stable at integrated in the world at present
The biogum that best performance is got over.The molecular side chain end of xanthan gum contains the number of pyruvic acid group, has very big shadow to its performance
It rings.Xanthan gum has the general performance of long chain macromolecule, but it contains more functional group than general macromolecule, in specified conditions
Under can show special performance.Its conformation in aqueous solution be it is various, different characteristics is not showed under the conditions of.1. suspension
And emulsibility: xanthan gum has good suspension effect to insoluble solid and oil droplet.Xanthan gum colloidal sol molecule can form superjunction
The spiral copolymer of crossed belt shape constitutes the reticular structure of fragile similar glue, so solid particle, drop and bubble can be supported
Form, show the effect of very strong stable emulsifying and uphang floating capacity.2. good water solubility: xanthan gum in water can be quick
Dissolution, there is good water solubility.It can also be dissolved especially in cold water, many and diverse process can be saved, it is easy to use.But due to
It has extremely strong hydrophily, stirs if being directly added into water insufficient, and outer layer water swelling can prevent moisture from entering at micelle
Inner layer, thus the performance of influence, it is therefore necessary to pay attention to proper use of.Xanthan gum dry powder or with the dry powder materials, stirring such as salt, sugar
It is slow after even to promote that the water stirred is added, solution use is made.3. thickening property: xanthan gum solution has the highly viscous spy of low concentration
Property (viscosity of 1% aqueous solution be equivalent to gelatin 100 times), is a kind of efficient thickener.4. pseudoplastic behavior: xanthan gum solution
There is high viscosity under static or low shear action, show as viscosity under high shear forces and sharply decline, but molecular structure
It is constant.And when shearing force is eliminated, then recover immediately original viscosity.The relationship of shearing force and viscosity is completely plastic.It is yellow
Virgin rubber pseudoplastic behavior is very prominent, and this pseudoplastic behavior is extremely effective to stable suspension, emulsion.5. pair hot stability: xanthan
The viscosity of sol solution will not variation with temperature and change a lot, general polysaccharide because heating viscosity change can occur,
But the aqueous solution of xanthan gum viscosity between 10-80 DEG C has almost no change, even if the aqueous solution of low concentration is in wide temperature
Still stable high viscosity is shown in range.1% xanthan gum solution, which is heated to 120 DEG C of its viscosity from 25 DEG C, only reduces by 3%.6.
To the stability of soda acid: xanthan gum solution is sufficiently stable to soda acid, makes its viscosity unaffected between 5-10 in PH, small in PH
In 4 and viscosity has slight variation when greater than 11.Within the scope of PH3-11, viscosity most ambassador and minimum value differ less than 10%.It is yellow
Virgin rubber can be dissolved in a variety of acid solutions, such as 5% sulfuric acid, 5% nitric acid, 5% acetic acid, 10% hydrochloric acid and 25% phosphoric acid, and these
Xanthan gum acid solution is quite stable at normal temperature, and several months long quality will not still change.Xanthan gum can also be dissolved in hydroxide
Sodium solution, and it is sufficiently stable at room temperature that there is thickening characteristic to be formed by solution.Xanthan gum can such as cross chlorine by strong oxidizer
Acid, over cure acid degradation, increase with temperature, and degradation accelerates.7. the stability of pair salt: xanthan gum solution can be mixed with many salting liquids
Molten, viscosity is unaffected.Still keep its dissolubility without heavy under the conditions of higher salt concentrations, or even in saturated salt solution
It forms sediment and flocculation, viscosity is barely affected.8. the stability of pair enzyme digestion reaction: the stable double-spiral structure of xanthan gum makes its tool
There are extremely strong anti-oxidant and resistance to enzymolysis ability, the enzymes such as many enzymes such as protease, amylase, cellulase and hemicellulase
Xanthan gum degradation cannot all be made.
The strain that xanthan gum can be produced at present is Xanthomonas campestris, and the yield and quality of xanthan gum is not only influenced by bacterial strain, and
And it is also influenced by nutriment type and condition of culture in culture medium.Xanthan gum is by D- glucose, D-MANNOSE, the Portugal D-
Grape uronic acid, acetyl group and pyruvic acid are constituted, the property of acetone acid content Different Effects xanthan gum.Higher acetone acid content is assigned
Highly viscous characteristic under the good pseudo plastic of xanthan gum and low shear rate is given, the content of pyruvic acid is equal in existing yellow glue original
It is lower, it is usually no more than 5%.Chinese invention patent " CN2017106658102 " discloses a kind of production of high-quality yellow virgin rubber
Method, this method improve acetone acid content in xanthan gum, content can by optimization fermentation medium and incubation step parameter
Up to 15-20%, but yield of xanthan gum is still to be improved in fermentation liquid, up to 20g/L or so.
Summary of the invention
Present invention aim to address xanthan gum fermentation culture medium costs in the prior art higher, xanthan gum yield and product
The lower defect of matter, provides the zymotechnique of low-cost high-quality xanthan gum.
The present invention is achieved by the following technical solution.
The zymotechnique of low-cost high-quality xanthan gum comprising following steps:
By Xanthomonas campestris access equipped with fermented and cultured is carried out in the fermentor of fermentation medium, fermentor and ceramic membrane are coupled,
Fermentation time is 48h, by the fermentation liquid in fermentor through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into
Concentration thallus is returned fermentor, while adding fermentation tank culture medium into fermentor by feed liquid storage tank, is made and without ceramic membrane mistake
Fermentating liquid volume before filter is identical, continues the 36-48h that ferments, obtains fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane point
From, obtain filtrate and concentration thallus, filtrate is drained into feed liquid storage tank.
Further, the zymotechnique includes the following steps:
Xanthomonas campestris seed liquor is equipped in the 30L fermentor of 21L fermentation medium according to the inoculum concentration access of 8-10% and is carried out
Fermented and cultured 30 DEG C of fermentation temperature, fermentor and ceramic membrane is coupled, fermentation time 48h, the fermentation liquid in fermentor is passed through
Ceramic membrane separation obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank, concentration thallus is returned fermentor, simultaneously
Fermentation tank culture medium is added into fermentor, make it is identical as without the fermentating liquid volume before ceramic membrane filter, continue ferment 36-
48h obtains fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into
Feed liquid storage tank；In entire fermentation process, by stream plus GPE defoaming, residual sugar control is being not less than by stream plus glucose solution
1.5-2% is 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level.
Preferably, the molecular cut off of the ceramic membrane is 10000-20000Da.
Preferably, the fermentation medium includes following component:
Glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, oleic acid 10g/L, calcium carbonate 3g/L, seven water sulphur
Sour magnesium 1g/L, dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
Preferably, the fermentation medium the preparation method comprises the following steps:
Take each raw material, be successively added in water, stir evenly, adjust pH to get.
Preferably, the thallus enzymolysis liquid the preparation method comprises the following steps: collect the thallus in xanthan gum fermentation broth, it is dry to moisture
Content is less than the dry mycelium of 5wt%, and being diluted with water to dry mycelium concentration is 40g/L, is placed in high-speed shearing machine with 10000rpm
Speed shear 120s, obtain bacteria suspension, the concentration that same volume is added into bacteria suspension is the hydrochloric acid solution of 1mol/L, mixed
It is even, 1h is handled at 95 DEG C, is added trypsase later and is hydrolyzed, then ceramic membrane filter, collects filtrate, 90 DEG C of enzyme deactivations
10min to get.
Preferably, the hydrolysising condition of the trypsase are as follows: pH 8, temperature be 37 DEG C, hydrolysis time 6h.
Preferably, the enzyme activity of the trypsase is 4000U/g.
Compared with prior art, the beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
The present invention avoids the shortage due to nutrients, living environment is deteriorated, xanthan gum in such a way that film is coupled dialysis fermentation
The problems in the fermentation such as feedback inhibition, improve fermentation efficiency, the total output of xanthan gum greatly improves；
Carbon source a part is used to constitute cell component, and a part maintains normal metabolism, and another part is produced for purpose
The synthesis of object.The present invention uses glucose and cornstarch mixed carbon source, saves cost of material, and bacterial strain preferentially uses glucose,
With the increase of bacterial strain concentration, the enzymes such as the amylase of secretion increase, and can digest cornstarch as carbon source；This method is with Huang
The discarded mycoprotein of virgin rubber fermentation is raw material, and fermentation medium, raw material is made as organic nitrogen source after trypsin hydrolysis
Thallus is left after fermentation, low in cost, compared with being used as feed, albumen potency is higher, and benefit is more preferable, can directly reduce
Product benefit improves in industrial cost.The nitrogen sources such as yeast extract are substituted by addition thallus enzymolysis liquid, can greatly save and be fermented into
This.
Addition glutamic acid can increase the yield of xanthan gum in the medium, and mycoprotein enzymolysis liquid of the present invention contains a large amount of paddy
Propylhomoserin (accounts for 10% of total amino acid or more), can increase the yield of xanthan gum.Calcium and magnesium inorganic ions also can to thalli growth and
Product formation has an impact, and for magnesium elements to the irritating effect of thalli growth, calcium carbonate is the weight for influencing yield of xanthan gum and quality
The factor is wanted, the synthesis of exoprotein can be reduced under the conditions of suitable, improves the yield of xanthan gum, in addition, calcium carbonate also has
There is buffered fermentation liquid pH.
Containing groups such as a large amount of phenolic hydroxyl groups, carbonyls in fulvic acid, electrolysis degree is higher, can promote xanthan gum synthesis process
In to O2Utilization, further increase gum yield and xanthan gum quality.Oleic acid can reduce gas-liquid and pass oxygen resistance, improve oxygen and pass
Matter rate enhances system oxygen delivery capacity, improves xanthan gum yield, and without being additionally provided energy.
It is more early that oxyty height starts the biosynthesis of xanthan gum, and the stopping that xanthan gum generates is often as
In culture medium caused by the exhausting of carbon source, in a sense, high concentration xanthan gum fermentation broth obtain to have it is higher
Premised on oxyty, but the dissolved oxygen amount of excessive concentrations also result in carbon source consumption it is too fast, generate more by-product, from
And conversion ratio is reduced, fermentation costs are improved, therefore, the dissolved oxygen of suitable concentration is an important factor for improving fermentation efficiency.
Detailed description of the invention
Fig. 1: influence of the different fermentations mode to yield of xanthan gum；
Fig. 2: influence of the oleic acid additive amount to yield of xanthan gum in culture medium；
Fig. 3: influence of the fulvic acid additive amount to yield of xanthan gum in culture medium；
Fig. 4: dissolved oxygen amount influences yield of xanthan gum.
Specific embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.Product and method of the invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified in bright content, spirit and scope or appropriate changes and combinations, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
The zymotechnique of low-cost high-quality xanthan gum comprising following steps:
17915 seed liquor (1 × 10 of Xanthomonas campestris ATCC8CFU/mL 21L) is housed according to the inoculum concentration access of 8% (volume ratio)
It carries out fermented and cultured in the 30L fermentor of fermentation medium, 30 DEG C of fermentation temperature, fermentor and ceramic membrane is coupled, when fermentation
Between be 48h, by the fermentation liquid in fermentor through ceramic membrane separation, obtain filtrate and concentration thallus, filtrate be drained into feed liquid storage
Concentration thallus is returned fermentor, while adding fermentation tank culture medium into fermentor by tank, make with without ceramic membrane filter before
Fermentating liquid volume it is identical, continue ferment 48h, obtain fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane separation, filtered
Liquid and concentration thallus, are drained into feed liquid storage tank for filtrate；In entire fermentation process, by stream plus GPE defoaming, pass through stream plus Portugal
Grape sugar juice, which controls residual sugar, is being not less than 2%, is 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level；It is described
The molecular cut off of ceramic membrane is 10000Da.
The fermentation medium includes following component:
Glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, oleic acid 10g/L, calcium carbonate 3g/L, seven water sulphur
Sour magnesium 1g/L, dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
The fermentation medium the preparation method comprises the following steps:
Take each raw material, be successively added in water, stir evenly, adjust pH to get；
The thallus enzymolysis liquid the preparation method comprises the following steps: collect the thallus in xanthan gum fermentation broth, it is dry to be less than to moisture content
The dry mycelium of 5wt%, being diluted with water to dry mycelium concentration is 40g/L, is placed in high-speed shearing machine and is cut with the speed of 10000rpm
120s is cut, bacteria suspension is obtained, the concentration that same volume is added into bacteria suspension is the hydrochloric acid solution of 1mol/L, is mixed, at 95 DEG C
Lower processing 1h adds trypsase later and is hydrolyzed, then ceramic membrane filter, collects filtrate, 90 DEG C of enzyme deactivation 10min to get；
The molecular cut off of ceramic membrane is 10000Da；Filtering removal is difficult to the macromolecular substances utilized by bacterial strain, including cell wall group
Point, high molecular weight protein etc..
The hydrolysising condition of the trypsase are as follows: pH 8, temperature be 37 DEG C, hydrolysis time 6h；The tryptose
The enzyme activity of enzyme is 4000U/g, additive amount are as follows: enzyme-to-substrate dry mass ratio is 1:30.
Embodiment 2
The zymotechnique of low-cost high-quality xanthan gum comprising following steps:
17915 seed liquor (1 × 10 of Xanthomonas campestris ATCC8CFU/mL 21L) is housed according to the inoculum concentration access of 10% (volume ratio)
It carries out fermented and cultured in the 30L fermentor of fermentation medium, 30 DEG C of fermentation temperature, fermentor and ceramic membrane is coupled, when fermentation
Between be 48h, by the fermentation liquid in fermentor through ceramic membrane separation, obtain filtrate and concentration thallus, filtrate be drained into feed liquid storage
Concentration thallus is returned fermentor, while adding fermentation tank culture medium into fermentor by tank, make with without ceramic membrane filter before
Fermentating liquid volume it is identical, continue ferment 36h, obtain fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane separation, filtered
Liquid and concentration thallus, are drained into feed liquid storage tank for filtrate；In entire fermentation process, by stream plus GPE defoaming, pass through stream plus Portugal
Grape sugar juice, which controls residual sugar, is being not less than 1.5%, is 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level；Institute
The molecular cut off for stating ceramic membrane is 20000Da.
The fermentation medium includes following component:
Glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, oleic acid 10g/L, calcium carbonate 3g/L, seven water sulphur
Sour magnesium 1g/L, dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
The fermentation medium the preparation method comprises the following steps:
Take each raw material, be successively added in water, stir evenly, adjust pH to get；
The thallus enzymolysis liquid the preparation method comprises the following steps: collect the thallus in xanthan gum fermentation broth, it is dry to be less than to moisture content
The dry mycelium of 5wt%, being diluted with water to dry mycelium concentration is 40g/L, is placed in high-speed shearing machine and is cut with the speed of 10000rpm
120s is cut, bacteria suspension is obtained, the concentration that same volume is added into bacteria suspension is the hydrochloric acid solution of 1mol/L, is mixed, at 95 DEG C
Lower processing 1h adds trypsase later and is hydrolyzed, then ceramic membrane filter, collects filtrate, 90 DEG C of enzyme deactivation 10min to get；
The molecular cut off of ceramic membrane is 10000Da；Filtering removal is difficult to the macromolecular substances utilized by bacterial strain, including cell wall group
Point, high molecular weight protein etc..
The hydrolysising condition of the trypsase are as follows: pH 8, temperature be 37 DEG C, hydrolysis time 6h；The tryptose
The enzyme activity of enzyme is 4000U/g, additive amount are as follows: enzyme-to-substrate dry mass ratio is 1:30.
Embodiment 3
Film is coupled influence of the dialysis fermentation to yield of xanthan gum
Experimental group is the fermentation method of embodiment 1；
Control group is normal fermentation mode, and fermentation time 72h, fermentation medium is the same as embodiment 1.
Fermentor is used with a batch of seed liquor, and indoor environment is identical, has comparability.
The xanthans content in the fermentation liquid of different time points (12,24,36,48,60,72,84,96h) is observed, it is conventional to send out
Ferment mode is generally 72h hours, because of overlong time, bacterial strain living environment is deteriorated, and extracellular yield of xanthan gum reaches certain
Threshold value, to Xanthan secretion generate feedback inhibition, therefore, continue extend fermentation time, yield of xanthan gum can not be promoted, such as
Shown in Fig. 1, experimental group avoids the shortage due to nutrients, living environment is deteriorated, yellow in such a way that film is coupled dialysis fermentation
The problems in fermentation such as feedback inhibition of virgin rubber, improves fermentation efficiency, and the total output of xanthan gum can reach 50.9g/L,
(first fermentation 25.8g/L+ ferment for the second time 25.1g/L), and normal fermentation is 31.1g/L, improves 63%.
Embodiment 4
Culture medium ferment effect test of the present invention.
Comparative example 1: glucose 100g/L, yeast extract 20g/L, oleic acid 10g/L, calcium carbonate 3g/L, epsom salt 1g/L,
Dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
Comparative example 2: glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, calcium carbonate 3g/L, seven water
Magnesium sulfate 1g/L, dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
Comparative example 3: glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, oleic acid 10g/L, carbonic acid
Calcium 3g/L, epsom salt 1g/L, dipotassium hydrogen phosphate 1g/L, VB120mg/L, pH 7.0-7.2.
Comparative example 4: glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, calcium carbonate 3g/L, seven water
Magnesium sulfate 1g/L, dipotassium hydrogen phosphate 1g/L, VB120mg/L, pH 7.0-7.2.
One, influence of the culture medium of embodiment 1 and comparative example 1-4 to xanthan gum.
Analyze influence of each culture medium to xanthan gum fermentation.Specific data target is shown in Table 1:
Table 1
| Index | Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 |
| Yield of xanthan gum g/L | 50.9 | 49.4 | 43.1 | 44.6 | 39.2 |
| Pyruvic acid functional group content % | 5.06 | 5.13 | 4.84 | 4.91 | 4.78 |
Conclusion: compared with comparative example 1, the present invention replaces common yeast extract using mycoprotein enzymolysis liquid, is formed sediment using corn
Powder substitutes part glucose, produces the not big influence of glue to fermentation, but fermentation costs greatly save, and main cause is bacterial enzyme
Solve the total free amino acid content highest in liquid, it is easier to be utilized by bacterial strain, and the content of total free amino acid Glutamic Acid
It is higher, it can reach 10% or more, have preferable facilitation to xanthan gum yield, and without additional addition pair in the medium
Xanthan gum fermentation has the glutamic acid of facilitation, reduces fermentation costs；Compared with comparative example 2-4, the present invention is produced in xanthan gum
Amount is substantially better than comparative example 2-4, and pyruvic acid functional group content also improves, it is seen then that oleic acid and fulvic acid are to fermentation
The quality for producing glue and xanthan gum has facilitation, and the two collaboration uses, and effect is more preferable.
Two, the influence of oleic acid and fulvic acid additive amount and oxyty to yield of xanthan gum in culture medium.
1, the additive amount of oleic acid is set as 0,2.5,5,10,20,40(g/L)；As shown in Fig. 2, with oleic acid additive amount
Increase, yield of xanthan gum gradually increases, and when to be added to 10g/L, yield of xanthan gum reaches peak value, continues growing the addition of oleic acid
Amount, the yield of xanthan gum have small size decline there is no increasing instead, and possible cause is that the excessive oleic acid that is added will lead to fermentation liquid
Water loss, and apparent viscosity increase causes the reduction of oxygen mass transfer coefficient, leads to the reduction of strain fermentation efficiency.
2, the additive amount of fulvic acid is set as 0,5,10,20,40,80(mg/L)；As shown in figure 3, as fulvic acid adds
The increase of amount, bacterial strain promote the utilization rate of oxygen, and correspondingly, yield of xanthan gum increases therewith, when to be added to 20mg/L, xanthan
Glue yield reaches peak value, continues growing the additive amount of oleic acid, and the yield of xanthan gum is not substantially change, selects adding for 20mg/L
Dosage is more appropriate.
3, dissolved oxygen amount influences yield of xanthan gum.
It is 5%, 10%, 15%, 20%, 25% that dissolved oxygen amount, which is arranged, 30% 6 gradient concentration, as shown in figure 4, dissolved oxygen amount increases,
The yield of xanthan gum increases therewith, and when dissolved oxygen amount increases to 20%, the yield of xanthan gum continues to increase dissolved oxygen close to maximum value
Amount, the amplification of xanthan gum are not obvious, but carbon source consumption rate significantly improves (rate of stream plus glucose increases), improves hair
Therefore ferment cost selects 20% or so dissolved oxygen amount the most suitable.
Listed above is only best specific embodiment of the invention.It is clear that the invention is not restricted to which above embodiments, may be used also
With there are many deformations.All changes that those skilled in the art directly can export or associate from present disclosure
Shape is considered as protection scope of the present invention.
Claims (8)
1. the zymotechnique of low-cost high-quality xanthan gum comprising following steps:
By Xanthomonas campestris access equipped with fermented and cultured is carried out in the fermentor of fermentation medium, fermentor and ceramic membrane are coupled,
Fermentation time is 48h, by the fermentation liquid in fermentor through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into
Concentration thallus is returned fermentor, while adding fermentation tank culture medium into fermentor by feed liquid storage tank, continues the 36-48h that ferments,
Fermentation liquid is obtained, by the fermentation liquid in fermentor through ceramic membrane separation, filtrate and concentration thallus is obtained, filtrate is drained into feed liquid
Storage tank.
2. zymotechnique according to claim 1, which is characterized in that the zymotechnique includes the following steps:
Xanthomonas campestris seed liquor is equipped in the 30L fermentor of 21L fermentation medium according to the inoculum concentration access of 8-10% and is carried out
Fermented and cultured 30 DEG C of fermentation temperature, fermentor and ceramic membrane is coupled, fermentation time 48h, the fermentation liquid in fermentor is passed through
Ceramic membrane separation obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank, concentration thallus is returned fermentor, simultaneously
Fermentation tank culture medium is added into fermentor, make it is identical as without the fermentating liquid volume before ceramic membrane filter, continue ferment 36-
48h obtains fermentation liquid, by the fermentation liquid in fermentor through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into
Feed liquid storage tank；In entire fermentation process, by stream plus GPE defoaming, residual sugar control is being not less than by stream plus glucose solution
1.5-2% is 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level.
3. zymotechnique according to claim 1 or 2, which is characterized in that the molecular cut off of the ceramic membrane is
10000-20000Da。
4. zymotechnique according to claim 1 or 2, which is characterized in that the fermentation medium includes following component:
Glucose 40g/L, cornstarch 60g/L, mycoprotein enzymolysis liquid 50g/L, oleic acid 10g/L, calcium carbonate 3g/L, seven water sulphur
Sour magnesium 1g/L, dipotassium hydrogen phosphate 1g/L, fulvic acid 20mg/L, VB120mg/L, pH 7.0-7.2.
5. zymotechnique according to claim 4, which is characterized in that the fermentation medium the preparation method comprises the following steps:
Take each raw material, be successively added in water, stir evenly, adjust pH to get.
6. zymotechnique according to claim 4 or 5, which is characterized in that the thallus enzymolysis liquid the preparation method comprises the following steps: receive
Collect the thallus in xanthan gum fermentation broth, be dried to obtain dry mycelium, being diluted with water to dry mycelium concentration is 40g/L, is placed in high speed and cuts
It cuts in machine and 120s is sheared with the speed of 10000rpm, obtain bacteria suspension, the concentration that same volume is added into bacteria suspension is 1mol/
The hydrochloric acid solution of L mixes, 1h is handled at 95 DEG C, adds trypsase later and is hydrolyzed, then ceramic membrane filter, collects
Filtrate, 90 DEG C of enzyme deactivation 10min to get.
7. zymotechnique according to claim 6, which is characterized in that the hydrolysising condition of the trypsase are as follows: pH 8,
Temperature is 37 DEG C, hydrolysis time 6h.
8. zymotechnique according to claim 6, which is characterized in that the enzyme activity of the trypsase is 4000U/g.
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