CN109928786A - A kind of microbial organic fertilizer and preparation method thereof - Google Patents
A kind of microbial organic fertilizer and preparation method thereof Download PDFInfo
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- CN109928786A CN109928786A CN201711457061.0A CN201711457061A CN109928786A CN 109928786 A CN109928786 A CN 109928786A CN 201711457061 A CN201711457061 A CN 201711457061A CN 109928786 A CN109928786 A CN 109928786A
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Abstract
The present invention relates to a kind of microbial organic fertilizers and preparation method thereof, belong to fertilizer field.Mentioned microorganism organic fertilizer includes by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, seven kinds of bacterium of saccharomyces cerevisiae form fermentation liquid after being sealed by fermentation according to the ratio, it is prepared as raw mixture according to the ratio with rice bran, Pig dung and urine, microelement after crushing including stalk, finally the mixed fermentation liquid of 7 kinds of bacterium is sufficiently stirred according to the ratio with the raw mixture, dried.The present invention matches scientific, content of organic matter height, and the fermentation of strain multiplicity is abundant, raw material environmental protection, fertilizer efficiency height and the ecological improvement soil that can adequately protect.
Description
[technical field]
The present invention relates to organic fertilizer field more particularly to a kind of microbial organic fertilizer and preparation method thereof
[background technique]
In the latest 20 years, industrial chemical fertilizer field of agricultural cultivation it is a large amount of use, exceeded use so that China, soil, farmland
Soil by unprecedented destruction, the acid-base property of generally existing soil is too high or too low, the farmland soil texture is viscous remakes
It is hardened at upper soll layer；Soil organic matter content is relatively low, influences the problems such as activity of microorganism in addition, single for a long time, excessive apply
With chemical fertilizer, humus cannot be replenished in time, and equally can also cause soil hardening, it is also possible to which be cracked
The soil of all soil hardenings, crop can cause root activity to decline because of anoxic, be unable to normal development change
The best approach of soil hardening is exactly that organic fertilizer and microbial manure are applied into soil exactly in such common recognition background
Under, various organic fertilizer bio-fertilizers come into being but biological organic fertilizer will be really achieved improvement soil, the supply enough nutrients of crop
Purpose, wherein the content of organic matter is crucial.
In existing biological organic fertilizer, there is a problem of that the content of organic matter is relatively low and content of beary metal is higher.In view of this,
The present invention provides the microbial organic fertilizer and preparation method that a kind of content of organic matter is high, content of beary metal is low, can be fundamentally
It improves soil and provides abundant nutrition to crop.
[summary of the invention]
The object of the present invention is to provide a kind of microbial organic fertilizers and preparation method thereof, improve soil and ecological environment, together
When the nutrition of part is filled to plant
A kind of microbial organic fertilizer, the raw material including following parts by weight:
A kind of microbial organic fertilizer, the microbial organic fertilizer include bacillus alcalophilus, bacillus licheniformis, withered grass bud
Spore bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae
Preferably, the microbial organic fertilizer bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae,
Thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mass ratio are as follows: (4.5~5): (1~1.5): (2~2.5): (0.5~1):
(0.5~1): (1~1.5): (0.5~1)
Preferably, the fowl and animal excrement includes but is not limited to pig manure
Preferably, the microelement includes nitrogen substance, phosphorus containg substances, substance containing potassium
Preferably, the microelement includes the mass ratio of nitrogen substance, phosphorus containg substances, the substance containing potassium are as follows: (4~4.5)
: (4~4.5): (1.5~2.5)
Present invention simultaneously provides a kind of preparation methods of microbial organic fertilizer, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mixes seven kinds of bacterium using mixed culture medium
Fermentation, fermentation time are 10-13 hours, and fermentation temperature is 50-55 DEG C
LB liquid medium (G/L): yeast powder 10G, peptone 10G, sodium chloride 10G are with oxychloride sodium adjustment pH value
7；
Seven kinds of bacterium mixed fermentive culture mediums (G/L): yeast powder 5G, bean cake powder 10G, potassium dihydrogen sulfate 0.5G, sulfate of ammoniac 1G,
Ferrous sulfate 0.05G is 7 with sodium hydroxide adjustment pH value；
2) preparation of raw mixture
It is mixed after stalk is crushed with rice bran, Pig dung and urine, microelement, wherein microelement, rice bran, stalk, Pig dung and urine
Mass ratio are as follows: 0.5: 4: 4: 1.5
3) into raw mixture be added by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae,
Thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, into machine seal stir, completely after mixing evenly,
Dry
Preferably, bacillus alcalophilus fermentation liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic unwrapping wire
Bacterium, thermophilic fungal, the fermentation liquid of saccharomyces cerevisiae co-fermentation and raw mixture mass ratio are as follows: 1~1.5: 8.5~9
Raw material drawing environmental protection of the present invention, wherein straw burns straw for a long time and makes mainly from Jiangnan rural area, Jiangnan rural area
At atmosphere pollution, and by straw as main raw material(s) of the invention, environment was both protected, also ensure that this hair to the maximum extent
Bright beneficial effect
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent, but embodiment is example sample, and it is not intended to limit the scope of the present invention in any way.
It is bacillus alcalophilus of the present invention, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic true
Bacterium, saccharomyces cerevisiae are commercially available
In embodiments of the present invention, fowl and animal excrement selects pig manure, is easy to get because pig manure is seen everywhere in rural area,
It not only while environmentally friendly, but also is one of the guarantee of beneficial effect of the present invention
In an embodiment of the present invention, microelement mainly includes nitrogen substance, phosphorus containg substances, substance containing potassium.In this hair
In bright embodiment, microbial bacteria fermentation liquid is sealed the machine of stirring with raw mixture
For the patent of invention special equipment of applicant
Embodiment 1
The microbial organic fertilizer includes 4.5 parts of bacillus alcalophilus, 1 part of bacillus licheniformis, bacillus subtilis 2
Part, 0.5 part of aspergillus oryzae, 0.5 part of thermophilic actinomycete, 1 part of thermophilic fungal, 0.5 part of saccharomyces cerevisiae
The microelement includes 2 parts of 4 parts of nitrogen substance, 4 parts of phosphorus containg substances, substance containing potassium
Present invention simultaneously provides a kind of preparation methods of microbial organic fertilizer, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mixes seven kinds of bacterium using mixed culture medium
Fermentation, fermentation time are 10 hours, and fermentation temperature is 50 DEG C
LB liquid medium (G/L): yeast powder 10G, peptone 10G, sodium chloride 10G are with oxychloride sodium adjustment pH value
7；
Seven kinds of bacterium mixed fermentive culture mediums (G/L): yeast powder 5G, bean cake powder 10G, potassium dihydrogen sulfate 0.5G, sulfate of ammoniac 1G,
Ferrous sulfate 0.05G is 7 with sodium hydroxide adjustment pH value；
2) preparation of raw mixture
It is mixed after stalk is crushed with rice bran, Pig dung and urine, microelement, wherein 0.5 part of microelement, 4 parts of rice bran, straw
4 parts of stalk, 1.5 parts of pig manure
3) into raw mixture be added by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae,
Thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, into machine seal stir, completely after mixing evenly,
Dry wherein, it is bacillus alcalophilus fermentation liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic
Fungi, 1 part of fermentation liquid of saccharomyces cerevisiae co-fermentation, raw mixture are 9 parts of
Embodiment 2
The microbial organic fertilizer includes 4.5 parts of bacillus alcalophilus, 1 part of bacillus licheniformis, bacillus subtilis 2
Part, 0.5 part of aspergillus oryzae, 0.5 part of thermophilic actinomycete, 1 part of thermophilic fungal, 0.5 part of saccharomyces cerevisiae
The microelement includes 4.5 parts of nitrogen substance, 4 parts of phosphorus containg substances, 1.5 parts of substance containing potassium.
A kind of preparation method of microbial organic fertilizer is provided simultaneously, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mixes seven kinds of bacterium using mixed culture medium
Fermentation, fermentation time are 11 hours, and fermentation temperature is 55 DEG C of
LB liquid medium (G/L): yeast powder 10G, peptone 10G, sodium chloride 10G are with oxychloride sodium adjustment pH value
7；
Seven kinds of bacterium mixed fermentive culture mediums (G/L): yeast powder 5G, bean cake powder 10G, potassium dihydrogen sulfate 0.5G, sulfate of ammoniac 1G,
Ferrous sulfate 0.05G is 7 with sodium hydroxide adjustment pH value；
2) preparation of raw mixture
It is mixed after stalk is crushed with Pig dung and urine, microelement, wherein 0.5 part of microelement, 8 parts of stalk, pig manure 1.5
Part, the present embodiment does not place rice bran, and raw material all uses straw, so the quality requirements to straw are higher.
It is added into raw mixture by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic
Hot actinomyces, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, into machine seal stir, completely after mixing evenly, dry in the air
Dry is wherein, bacillus alcalophilus fermentation liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic true
Bacterium, saccharomyces cerevisiae co-fermentation fermentation liquid be 1.5 parts, raw mixture be 8.5 parts of
Embodiment 3
The microbial organic fertilizer includes 4.5 parts of bacillus alcalophilus, 1 part of bacillus licheniformis, bacillus subtilis 2
Part, 0.5 part of aspergillus oryzae, 0.5 part of thermophilic actinomycete, 1 part of thermophilic fungal, 0.5 part of saccharomyces cerevisiae
The microelement includes 1.5 parts of 4 parts of nitrogen substance, 4.5 parts of phosphorus containg substances, substance containing potassium
A kind of preparation method of microbial organic fertilizer is provided simultaneously, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mixes seven kinds of bacterium using mixed culture medium
Fermentation, fermentation time are 12 hours, and fermentation temperature is 53 DEG C of
LB liquid medium (G/L): yeast powder 10G, peptone 10G, sodium chloride 10G are with oxychloride sodium adjustment pH value
7；
Seven kinds of bacterium mixed fermentive culture mediums (G/L): yeast powder 5G, bean cake powder 10G, potassium dihydrogen sulfate 0.5G, sulfate of ammoniac 1G,
Ferrous sulfate 0.05G is 7 with sodium hydroxide adjustment pH value；
2) preparation of raw mixture
It is mixed after stalk is crushed, with Pig dung and urine, microelement, wherein 0.5 part of microelement, 8 parts of stalk, pig manure
1.5 parts, the present embodiment does not also place rice bran, and raw material all uses straw, so the quality requirements to straw are higher.
3) into raw mixture be added by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae,
Thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, into machine seal stir, completely after mixing evenly,
It dries.Wherein, bacillus alcalophilus fermentation liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic
Fungi, 1 part of fermentation liquid of saccharomyces cerevisiae co-fermentation, raw mixture are 9 parts.
Embodiment 4
4.5 parts of the microbial organic fertilizer bacillus alcalophilus, 1 part of bacillus licheniformis, bacillus subtilis 2 part, meter
0.5 part of aspergillus, 0.5 part of thermophilic actinomycete, 1 part of thermophilic fungal, 0.5 part of saccharomyces cerevisiae
It include 4 parts of nitrogen substance, 4.5 parts of phosphorus containg substances, 1.5 parts of substance containing potassium including the microelement.
Present invention simultaneously provides a kind of preparation methods of microbial organic fertilizer, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mixes seven kinds of bacterium using mixed culture medium
Fermentation, fermentation time are 13 hours, and fermentation temperature is 54 DEG C of
LB liquid medium (G/L): yeast powder 10G, peptone 10G, sodium chloride 10G are with oxychloride sodium adjustment pH value
7；
Seven kinds of bacterium mixed fermentive culture mediums (G/L): yeast powder 5G, bean cake powder 10G, potassium dihydrogen sulfate 0.5G, sulfate of ammoniac 1G,
Ferrous sulfate 0.05G is 7 with sodium hydroxide adjustment pH value；
2) preparation of raw mixture
It is mixed after stalk is crushed with rice bran, Pig dung and urine, microelement, wherein 0.5 part of microelement, 4 parts of stalk, rice
4 parts of chaff, 1.5 parts of pig manure
3) into raw mixture be added by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae,
Thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, into machine seal stir, completely after mixing evenly,
It dries.Wherein, bacillus alcalophilus fermentation liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic
Fungi, 1.5 parts of fermentation liquid of saccharomyces cerevisiae co-fermentation, raw mixture are 8.5 parts of
Test example
By the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4 (by Guangzhou Tian Yue agricultural science and technology Co., Ltd reality
Apply production) use as base manure after, apply fertilizer 500KG per acre, by taking leaf mustard, romaine lettuce and cabbage heart as an example, and other fertilizer and does not apply
Fertilizer-plant compares, considerably shorter using the plant growing cycle of product of the embodiment of the present invention and yield obviously increases such as table
One: growth cycle table and table two: test rate schedule, such as table three: organic matter and nutrient detect table
Growth cycle table
Unit: day
Table one
Test rate schedule
Unit: kilogram
Table two
Meanwhile beneficial effect in order to further illustrate the present invention, now by embodiment 1, embodiment 2, embodiment 3, implementation
The organic matter and Results of Trace Element Detection of example 4 (implementing to produce by Guangzhou Tian Yue agricultural science and technology Co., Ltd) are enclosed, with drying
Base meter, national standard require the organic matter score of organic fertilizer to be >=45%, and the requirement to total nutrient is more than or equal to 5.0%.
And the testing result of the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4 is shown, organic matter score of the invention be >=
87%, the test result to total nutrient is more than or equal to 8.9%. such as table three:
Organic matter and nutrient detect table
Table three
In conclusion the present invention matches science, the fermentation of strain multiplicity sufficiently, raw material environmental protection, fertilizer efficiency is high and can sufficiently protect
Probationer nurse state improves soil.
Embodiments described above is only some of the invention, and based on the embodiments of the present invention, this field is common
Technical staff does not make all other embodiment obtained under the premise of creative work, belongs to the range of this bright protection.
Claims (7)
1. a kind of microbial organic fertilizer, it is characterised in that: the microbial organic fertilizer includes bacillus alcalophilus, lichens gemma bar
Bacterium, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae.
2. a kind of microbial organic fertilizer according to claim 1, it is characterised in that: the thermophilic gemma of microbial organic fertilizer
Bacillus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae mass ratio are as follows:
(4.5~5): (1~1.5): (2~2.5): (0.5~1): (0.5~1): (1~1.5): (0.5~1).
3. a kind of microbial organic fertilizer according to claim 1, it is characterised in that: the microelement include nitrogen substance,
Phosphorus containg substances, object containing potassium.
4. a kind of microbial organic fertilizer according to claim 3, it is characterised in that: the microelement include nitrogen substance,
The mass ratio of phosphorus containg substances, the substance containing potassium are as follows: (4~4.5): (4~4.5): (1.5~2.5).
5. a kind of preparation method of microbial organic fertilizer according to claim 1, the specific steps are as follows:
1) preparation of fermentation liquid passes through LB liquid medium culture bacillus alcalophilus, bacillus licheniformis, withered grass gemma respectively
After bacillus, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, saccharomyces cerevisiae carries out mixed fermentation to seven kinds of bacterium using mixed culture medium,
Fermentation time is 10-13 hours, and fermentation temperature is 50-55 DEG C.
2) preparation of raw mixture
It is mixed after rice bran and stalk or stalk are crushed with Pig dung and urine, microelement, microelement, rice bran and stalk, Pig dung and urine
Mass ratio are as follows: 0.5: 4: 4: 1.5
3) it is added into raw mixture by bacillus alcalophilus, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic
Actinomyces, thermophilic fungal, saccharomyces cerevisiae co-fermentation fermentation liquid, be then stirred, completely after mixing evenly, dry.
6. a kind of preparation method of microbial organic fertilizer according to claim 5, it is characterised in that: thermophilic in the step 3)
Hot fermentation of bacillus liquid, bacillus licheniformis, bacillus subtilis, aspergillus oryzae, thermophilic actinomycete, thermophilic fungal, wine brewing ferment
The fermentation liquid of female co-fermentation and the mass ratio of raw mixture are as follows: 1~1.5: 8.5~9.
7. a kind of preparation method of microbial organic fertilizer according to claim 5, it is characterised in that: in the raw mixture
Microelement, stalk, pig urine excrement mass ratio can also include: 0.5: 8: 1.5.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698237A (en) * | 2019-11-25 | 2020-01-17 | 湖南明镜生物科技有限公司 | Production method for producing organic fertilizer by using pig manure |
| CN111410566A (en) * | 2020-05-18 | 2020-07-14 | 上海净业农业科技发展有限公司 | Microbial compound fertilizer and preparation method thereof |
| CN112645746A (en) * | 2020-12-25 | 2021-04-13 | 东北农业大学 | Animal manure straw bio-organic fertilizer and preparation method thereof |
| CN113979793A (en) * | 2021-11-18 | 2022-01-28 | 耿海林 | Compound microbial fermentation organic fertilizer and preparation method thereof |
| CN117285382A (en) * | 2023-11-22 | 2023-12-26 | 海南热带海洋学院 | Method for multi-strain fermentation of fish manure and waste low-odor fertilizer based on neural network |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110698237A (en) * | 2019-11-25 | 2020-01-17 | 湖南明镜生物科技有限公司 | Production method for producing organic fertilizer by using pig manure |
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| CN112645746A (en) * | 2020-12-25 | 2021-04-13 | 东北农业大学 | Animal manure straw bio-organic fertilizer and preparation method thereof |
| CN113979793A (en) * | 2021-11-18 | 2022-01-28 | 耿海林 | Compound microbial fermentation organic fertilizer and preparation method thereof |
| CN117285382A (en) * | 2023-11-22 | 2023-12-26 | 海南热带海洋学院 | Method for multi-strain fermentation of fish manure and waste low-odor fertilizer based on neural network |
| CN117285382B (en) * | 2023-11-22 | 2024-04-05 | 海南热带海洋学院 | Method for multi-strain fermentation of fish manure and waste low-odor fertilizer based on neural network |
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