CN109916782A - A method of it is detected using miillpore filter - Google Patents
A method of it is detected using miillpore filter Download PDFInfo
- Publication number
- CN109916782A CN109916782A CN201910188816.4A CN201910188816A CN109916782A CN 109916782 A CN109916782 A CN 109916782A CN 201910188816 A CN201910188816 A CN 201910188816A CN 109916782 A CN109916782 A CN 109916782A
- Authority
- CN
- China
- Prior art keywords
- miillpore filter
- pad pasting
- detected
- miillpore
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of methods detected using miillpore filter to be added to pad pasting liquid on glass slide before the ingredient that detection miillpore filter is retained, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves;Miillpore filter after the completion of preparation forms colorless and transparent homogeneous body.The present invention provides a kind of method detected using miillpore filter, can dissolve miillpore filter, in favor of observing, detecting the cell or other molecules on miillpore filter.
Description
Technical field
The present invention relates to medical detection field, specially a kind of method detected using miillpore filter.
Background technique
When carrying out micro- sem observation to cell or other molecules, in order to smoothly observe these under the microscope
Fine particle, often requires to use oil mirror and amplification factor needs are even higher at 1000 times.At this time if miillpore filter is too thick,
Often layering is serious in microscopy for object, result judgement is not easy to, so the miillpore filter thickness used is often micro- in 20-50
Within rice.The existing method such as Chinese Patent Application No. detected using miillpore filter: disclosed in 201110462479.7
A kind of detection method and film transparent liquid using UF membrane, in the forming process that is retained of detection miillpore filter, using film
Transparent liquid is added on filter membrane, so that the filter membrane shows transparent state;This method it is practical but still
Have deficiency, need to wait for miillpore filter when such as detecting and be completely dried, remain on another aspect miillpore filter other electrolyte at
It is transparent not exclusively that timesharing will lead to miillpore filter, so still having room for improvement.
Summary of the invention
The present invention provides a kind of method detected using miillpore filter, can dissolve miillpore filter, in favor of seeing
It examines, detect cell or other molecules on miillpore filter.
In order to achieve the above object, the technical solution adopted by the present invention is that: it, will before the ingredient that detection miillpore filter is retained
Pad pasting liquid is added on glass slide, and then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves;After the completion of preparation
Miillpore filter form colorless and transparent homogeneous body.
Further, the material of the miillpore filter is Kynoar (PVDF), with a thickness of 20 microns.
Further, the pad pasting liquid material is the organic solvent of high concentration.
Further, the organic solvent is dimethylformamide or dimethyl acetamide.
Beneficial effects of the present invention: pad pasting liquid is water-soluble and can quickly dissolve miillpore filter, after miillpore filter dissolution
Colorless and transparent homogeneous body is formed, convenient for insoluble to the cell or other molecules retained on miillpore filter etc. under the microscope
The visible component of solution is detected, and is avoided miillpore filter opaque and is influenced detection effect, while complete without waiting for miillpore filter
White drying can be observed, be detected, and reduce the waiting time to improve detection efficiency.
1, miillpore filter is dissolved as colorless and transparent liquid under the action of pad pasting liquid, and coverslip lid is closed on glass slide
When, it can be ensured that coverslip can press very flat, it is ensured that detection quality.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, the present invention is retouched in detail below
State, the description of this part be only it is exemplary and explanatory, should not have any restriction effect to protection scope of the present invention.
It is of the invention the specific scheme is that pad pasting liquid is added to glass slide before the ingredient that detection miillpore filter is retained
On, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves;Miillpore filter after the completion of preparation forms colourless
Clear homogeneous body.
Preferably, the material of the miillpore filter is Kynoar (PVDF), with a thickness of 20 microns.
Preferably, the pad pasting liquid material is the organic solvent of high concentration.
Preferably, the organic solvent is dimethylformamide or dimethyl acetamide.
Specific embodiment: will be mounted in specimen collecting device using miillpore filter made of Kynoar (PVDF),
Then the sample in collection device is collected and the cell or other molecules that are adhered on miillpore filter is contaminated
Color, cleaning and etc., another Chinese invention patent of the applicant, Patent No. can be used in specific steps
Other methods can also be used in technical solution described in CN201010529398.X;Dyeing, cleaning and etc. after the completion of, carry glass
The pad pasting liquid made of dimethylformamide or dimethyl acetamide is added dropwise on piece, then filters the micropore in specimen collecting device
Film is removed and placed to be covered on coverslip micro- after pad pasting liquid dissolves miillpore filter in the pad pasting liquid zone domain on glass slide
The film-making of sample is completed on the lysigenous region of hole filter membrane.When detection, by sample be placed under microscope with to cell or its
The undissolved visible component such as its molecule is detected.
Above to it is provided by the present invention it is a kind of be described in detail using the method that miillpore filter is detected, this
Principle and implementation of the present invention are described in text, the present invention that the above embodiments are only used to help understand
Method with its core concept;At the same time, for those skilled in the art is being embodied according to the thought of the present invention
There will be changes in mode and application range, in conclusion the content of this specification should not be construed as to limit of the invention
System.
Claims (4)
1. a kind of method detected using miillpore filter, which is characterized in that before the ingredient that detection miillpore filter is retained,
Pad pasting liquid is added on glass slide, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves;Preparation is completed
Miillpore filter afterwards forms colorless and transparent homogeneous body.
2. a kind of method detected using miillpore filter according to claim 1, which is characterized in that the micropore filter
The material of film is Kynoar, with a thickness of 20 microns.
3. a kind of method detected using miillpore filter according to claim 1, which is characterized in that the pad pasting liquid
Material is the organic solvent of high concentration.
4. a kind of method detected using miillpore filter according to claim 3, which is characterized in that described organic molten
Agent is dimethylformamide or dimethyl acetamide.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910188816.4A CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910188816.4A CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Publications (1)
Publication Number | Publication Date |
---|---|
CN109916782A true CN109916782A (en) | 2019-06-21 |
Family
ID=66964555
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910188816.4A Pending CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109916782A (en) |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101508457A (en) * | 2009-03-20 | 2009-08-19 | 华中科技大学 | Method for preparing ZnO thick film |
CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
CN102443274A (en) * | 2011-09-21 | 2012-05-09 | 中国科学院苏州纳米技术与纳米仿生研究所 | CNT (carbon nano tube)/macromolecule composite film and preparation method thereof |
CN102580548A (en) * | 2011-12-20 | 2012-07-18 | 湖南省天骑医学新技术有限公司 | Detection method applied to membrane separation and membrane transparent liquid |
CN103728289A (en) * | 2013-12-16 | 2014-04-16 | 金川集团股份有限公司 | Method for rapidly measuring gold and silver in crude copper |
CN103884554A (en) * | 2014-03-14 | 2014-06-25 | 中国科学院东北地理与农业生态研究所 | Method for separating PM2.5 dust from Teflon filter membrane |
CN103884630A (en) * | 2014-03-13 | 2014-06-25 | 河北联合大学 | Determination method for dispersity of dust in air of working field |
CN104726337A (en) * | 2013-12-20 | 2015-06-24 | 中国科学院生态环境研究中心 | Method for enriching and purifying cryptosporidium and giardia in alga-containing water, and method for determining content of cryptosporidium and giardia |
CN105597571A (en) * | 2016-02-29 | 2016-05-25 | 天津大学 | High-strength self-supporting ultrafiltration membrane prepared through vacuum auxiliary self-assembly and preparation method |
CN106226896A (en) * | 2016-08-08 | 2016-12-14 | 湖南省天骑医学新技术股份有限公司 | A kind of can be with the microscope mirror oil compound method of transparent microporous filter membrane |
CN107096396A (en) * | 2017-05-19 | 2017-08-29 | 开源环保(集团)有限公司 | A kind of polyvinylidene fluoride hollow fiber miillpore filter and preparation method thereof |
-
2019
- 2019-03-13 CN CN201910188816.4A patent/CN109916782A/en active Pending
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101508457A (en) * | 2009-03-20 | 2009-08-19 | 华中科技大学 | Method for preparing ZnO thick film |
CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
CN102443274A (en) * | 2011-09-21 | 2012-05-09 | 中国科学院苏州纳米技术与纳米仿生研究所 | CNT (carbon nano tube)/macromolecule composite film and preparation method thereof |
CN102580548A (en) * | 2011-12-20 | 2012-07-18 | 湖南省天骑医学新技术有限公司 | Detection method applied to membrane separation and membrane transparent liquid |
CN103728289A (en) * | 2013-12-16 | 2014-04-16 | 金川集团股份有限公司 | Method for rapidly measuring gold and silver in crude copper |
CN104726337A (en) * | 2013-12-20 | 2015-06-24 | 中国科学院生态环境研究中心 | Method for enriching and purifying cryptosporidium and giardia in alga-containing water, and method for determining content of cryptosporidium and giardia |
CN103884630A (en) * | 2014-03-13 | 2014-06-25 | 河北联合大学 | Determination method for dispersity of dust in air of working field |
CN103884554A (en) * | 2014-03-14 | 2014-06-25 | 中国科学院东北地理与农业生态研究所 | Method for separating PM2.5 dust from Teflon filter membrane |
CN105597571A (en) * | 2016-02-29 | 2016-05-25 | 天津大学 | High-strength self-supporting ultrafiltration membrane prepared through vacuum auxiliary self-assembly and preparation method |
CN106226896A (en) * | 2016-08-08 | 2016-12-14 | 湖南省天骑医学新技术股份有限公司 | A kind of can be with the microscope mirror oil compound method of transparent microporous filter membrane |
CN107096396A (en) * | 2017-05-19 | 2017-08-29 | 开源环保(集团)有限公司 | A kind of polyvinylidene fluoride hollow fiber miillpore filter and preparation method thereof |
Non-Patent Citations (5)
Title |
---|
LEOPOLD G.KOSS 等: "《KOSS诊断细胞学及其组织病理学基础》", 30 June 2009, 世界图书出版西安公司 * |
无: "粉尘分散度滤膜溶解涂片改良法", 《工业卫生与职业病》 * |
潘勇 等: "《水产品质量检验员》", 30 September 2016, 海洋出版社 * |
王又蓉: "《膜技术问答》", 31 January 2007, 国防工业出版社 * |
王芳: "《细胞病理学》", 31 January 2017, 天津科学技术出版社 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Patel et al. | Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I | |
CN109253907B (en) | Method for rapidly and auxiliarily detecting micro-plastic in water environment sample by using Nile red dyeing | |
US10890513B2 (en) | Cell-holding substrate holder for preparing observation specimen, kit including same, and observation specimen preparation method | |
CN1764469A (en) | Papanicolau staining process | |
CN105738182A (en) | Fluorescent staining method for observing plant microstructure | |
RU2627395C2 (en) | Membrane separation method and transparent liquid for the membrane | |
WO2022242398A1 (en) | Pretreatment reagent, preparation method, cell staining method and pretreatment method | |
CN109916782A (en) | A method of it is detected using miillpore filter | |
CN106289906A (en) | A kind of microporous filter membrane pelletizer and the assembly method when film-making and microscope are observed thereof | |
Dell'Anno et al. | Determination of viral production in aquatic sediments using the dilution-based approach | |
CN1908621A (en) | Giardia sporangiocyst in water and Cryptosporidium hominis egg capsule checking method | |
WO2017047617A1 (en) | Cell-holding substrate holder for preparing observation specimen, kit including same, and observation specimen preparation method | |
CN106932322A (en) | A kind of interior wall tests the method and apparatus of contaminated resistance | |
CN106323723A (en) | Double-blue staining method | |
CN112284861B (en) | Fixing liquid for hypsizigus marmoreus basidiospore microscopic observation, preparation method, fixing method and application | |
CN106226896B (en) | It is a kind of can be with the microscope mirror oil preparation method of transparent miillpore filter | |
JP2017058152A (en) | Method for creating microscopic examination specimen | |
CN201689002U (en) | Automatic staining device adopting two-step Gram stain | |
JP2007202472A (en) | Cell fixation solution, method and kit for cell fixation using the same | |
CN112577794A (en) | Method for preparing film-taking enriched bacteria by shadowless adhesive and application thereof | |
US20240230484A9 (en) | Hydrogel-based stamping for solution-free blood cell staining | |
CN214794098U (en) | Pathological glass slide suitable for tiny specimen | |
CN103993430B (en) | The preparation method of a kind of colorimetric nano-fiber film and the application for cadmium ion detection thereof | |
CN207894698U (en) | Cell smear staining rack | |
CN102042924B (en) | A kind of cell or the dyeing of antibacterial, flaking method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190621 |