CN109913977A - A kind of nucleic acid gel fiber and preparation method thereof - Google Patents
A kind of nucleic acid gel fiber and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to technical field of biological materials, in particular to a kind of nucleic acid gel fiber and preparation method thereof.Nucleic acid is reacted to obtain compound by electrostatic in water phase with cationic surfactant by preparation method of the present invention, and through centrifugation, washing is added organic solvent, obtains nucleic acid gel fiber after swelling, wire drawing after freeze-drying.Nucleic acid gel fiber produced by the present invention has a high tensile, high-modulus, excellent ductility, and liquid crystal structure is kept in organic phase, and toughness can match in excellence or beauty natural spider's thread fiber.Preparation method simple process of the present invention simultaneously is not necessarily to special installation, low in cost.
Description
Technical field
The present invention relates to technical field of biological materials more particularly to a kind of nucleic acid gel fiber and preparation method thereof.
Background technique
Nucleic acid is that one kind is usually located at endonuclear macro-organism molecule, be responsible for organism hereditary information carrying and
Transmitting, and can be used as the structural motif of nanostructure assembling, it is always big point of biology of technical field of biological materials most study
One of son.In recent years, due to automating synthesis in solid state, molecule clone technology and the mature of polymerase chain reaction develop, so that
The research of short-chain nucleic acids and long nucleic acid is possibly realized.Due to the unique property of nucleic acid, such as Urine scent ability, sequence can be compiled
Journey and excellent stability.Become the hot spot of the biomaterial research field of functionalization.Wherein, due to its sequence
The programmability of length and base makes nucleic acid be widely used in drug delivery, the fields such as gene conduction and detection technique.
The soft substance of macromolecular, such as liquid crystal, hydrogel and organogel, due to its excellent performance and functional diversity,
People have been caused more and more to pay close attention to.Wherein, the liquid crystal and hydrogel material prepared using nucleic acid as matrix is especially prominent.
The molecule distinguishability of nucleic acid makes it have programmability, can be applied to stimulation respective material and biomedical applications.This property
The nucleic acid that can be has a huge application prospect as soft substance, but poor mechanical property, hinders it and is needing machinery
Practical application in the field of integrality and adjustability.In addition, the gel rubber system based on nucleic acid is mostly amorphous phase material,
Lack orderly internal structure in gel volume grid, closely hinders it in anisotropy electricity, optics, magnetics or mechanicalness
Research in terms of matter.Thus, the high-intensitive nucleic acid gel with liquid crystal structure how is prepared to basic scientific research and technical application
It has practical significance.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of nucleic acid gel fibers and preparation method thereof.Core made from this
Acid cure glue fiber is liquid crystal structure, has excellent tensile strength and ductility.
In order to achieve the object of the present invention, the present invention adopts the following technical scheme:
The present invention provides a kind of preparation method of nucleic acid gel fiber, comprising:
It takes nucleic acid to mix, react with cationic surfactant, obtains compound；
Organic solvent is added into the compound to be swollen, nucleic acid gel is obtained；
By the nucleic acid gel wire drawing, nucleic acid gel fiber is obtained.
In preparation method provided by the invention, nucleic acid is reacted in water phase by electrostatic interaction with cationic surfactant
Obtain compound.
Before carrying out the reaction, nucleic acid solution first is made in nucleic acid then add cationic surfactant into
Row reaction.Nucleic acid can be dissolved in ultrapure water and nucleic acid solution is made, nucleic acid can also be dissolved in PBS buffer solution, nucleic acid is made
Solution.
In certain embodiments, the content of the nucleic acid solution amplifying nucleic acid is 2~10mg/ml.In some specific realities
It applies in example, the content of the nucleic acid solution amplifying nucleic acid is 10mg/ml, 5mg/ml or 2mg/ml.
In some embodiments, in terms of base number, the molar ratio of the nucleic acid and cationic surfactant is nucleic acid
1:1~1:10.In certain embodiments, the molar ratio of nucleic acid and cationic surfactant is 1:5.
In some embodiments, nucleic acid is long-chain DNA, the long-chain DNA containing 200~
2686 base-pairs.In certain embodiments, long-chain DNA contains 200,500,1000,2000 or 2686
Base-pair.
In some embodiments, nucleic acid is short chain DNA, and the short chain DNA contains 14~22
A base-pair.In certain embodiments, the short chain DNA contains 14 or 22 base-pairs.
In some embodiments, cationic surfactant is selected from didodecyldimethylammbromide bromide, cetyl
One of trimethylammonium bromide or didecyldimethylammonium bromide are a variety of.
In certain embodiments, cationic surfactant is didodecyldimethylammbromide bromide, cetyl
Trimethylammonium bromide or didecyldimethylammonium bromide.
After obtaining compound, first by the compound centrifugation, wash, freeze-drying.In certain embodiments,
The centrifugation is that 10000rpm is centrifuged 30 minutes；The washing is washed with deionized to adopt, and washing times are 3 times；The jelly
It does as compound to be placed in freeze-drying instrument overnight, or compound is first placed in liquid nitrogen and is freezed, place into freeze-drying instrument and be lyophilized two
Hour.
After compound after being lyophilized, organic solvent is added and is swollen.In some embodiments, You Jirong
Agent is selected from dimethyl sulfoxide, tetrahydrofuran, chloroform, toluene, ethyl alcohol, one of ethylene glycol or glycerine or a variety of.Some
In specific embodiment, organic solvent is toluene, dimethyl sulfoxide, tetrahydrofuran or ethyl alcohol.
In some embodiments, the time of swelling is 10min~2h, preferably 30min or 60min.
The present invention also provides nucleic acid gel fibers made from the preparation method.
Nucleic acid of the present invention reacts to obtain compound by electrostatic in water phase with cationic surfactant, through centrifugation, washes
It washs, organic solvent is added after freeze-drying, obtains nucleic acid gel fiber after swelling, wire drawing.The invention has the following advantages:
1, products therefrom of the present invention has liquid crystal structure, and has excellent tensile strength and ductility.Resulting core
Acid gel is extensible to 300 times of length or more.
2, the resulting nucleic acid gel compound with regular structure of the present invention, mechanical performance is excellent, will become building new type functional nucleic acid
The starting point of material widens the research range of nucleic acid nano structure, or even operable molecular material is realized in organic environment.
3, preparation process of the present invention it is simple, it is low in cost, be not necessarily to special installation.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will to embodiment or
Attached drawing needed to be used in the description of the prior art is briefly described.
Fig. 1 is the swelling figure of 1 nucleic acid gel of embodiment；
Fig. 2 is the ductility test chart of 2 nucleic acid gel fiber of embodiment, and wherein Fig. 2-A is nucleic acid made from embodiment 2
Gel, Fig. 2-B are that nucleic acid gel extends into about 80 times of length, and Fig. 2-C is that nucleic acid gel extends into length about 170
Times, Fig. 2-D is that nucleic acid gel extends into about 330 times of length；
Fig. 3 is the mechanical property figure of 3 nucleic acid gel fiber of embodiment, wherein Fig. 3-A is the Young of nucleic acid gel fiber
Modulus measurement result figure, Fig. 3-B are the toughness measurement chart of nucleic acid gel fiber.
Specific embodiment
The invention discloses a kind of nucleic acid gel fiber and preparation method thereof, those skilled in the art can be used for reference herein
Content is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to art technology
It is it will be apparent that they are considered as being included in the present invention for personnel.Method and application of the invention has passed through preferably
Embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to side as described herein
Method and application are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
To the explanation of the disclosed embodiments, enable those skilled in the art to implement or use the present invention.To this
A variety of modifications of a little embodiments will be readily apparent to those skilled in the art, and as defined herein one
As principle can realize in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase
Consistent widest scope.
The test material that the present invention uses is all common commercially available product, can all be bought in market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
Take double dodecyl dimethyl bromines of 22bp (the 22 base-pairs) DNA solution and 5mL 50mM of 210 μ L5.4mM
Change ammonium to be put into centrifuge tube, concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in the centrifugation of 10000rpm
It is centrifuged 30 minutes under speed, removes supernatant, be centrifuged after deionized water washing is added, wash and move back three times except supernatant repeatedly,
Centrifugation gained complex precipitate is put into liquid nitrogen and is freezed, places into freeze-drying instrument and is lyophilized two hours.Then sample after freeze drying
The toluene of 100 μ L is added in product, places 30 minutes at room temperature, obtains nucleic acid gel, nucleic acid gel wire drawing acquisition nucleic acid is coagulated
Glue fiber.Fig. 1 is nucleic acid gel manufactured in the present embodiment, illustrates that nucleic acid gel of the invention has liquid crystal structure.
Embodiment 2
Take the DNA solution (200 base-pairs) of 210 μ L5.4mM and 5mL 50mM double dodecyl dimethyl brominations
Ammonium is put into centrifuge tube, and concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in the centrifugation speed of 10000rpm
Degree lower centrifugation 30 minutes, supernatant is removed, is centrifuged after deionized water washing is added, washs and moves back three times except supernatant repeatedly, it will
Centrifugation gained complex precipitate, which is put into liquid nitrogen, to be freezed, and is placed into freeze-drying instrument and is lyophilized two hours.Then sample after freeze drying
The middle toluene that 100 μ L are added, places 30 minutes at room temperature, obtains nucleic acid gel, and nucleic acid gel wire drawing is obtained nucleic acid gel
Fiber.Fig. 1 is nucleic acid gel manufactured in the present embodiment, illustrates that nucleic acid gel of the invention has liquid crystal structure.
Embodiment 3
Take double dodecyl dimethyl brominations of DNA solution (1000 base-pairs) and 5mL 50mM of 210 μ L5.4mM
Ammonium is put into centrifuge tube, and concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in the centrifugation speed of 10000rpm
Degree lower centrifugation 30 minutes, supernatant is removed, is centrifuged after deionized water washing is added, washs and moves back three times except supernatant repeatedly, it will
Centrifugation gained complex precipitate, which is put into liquid nitrogen, to be freezed, and is placed into freeze-drying instrument and is lyophilized two hours.Then sample after freeze drying
The middle toluene that 100 μ L are added, places 30 minutes at room temperature, obtains nucleic acid gel, and nucleic acid gel wire drawing is obtained nucleic acid gel
Fiber.Fig. 2 is nucleic acid gel extensive experiments manufactured in the present embodiment, illustrates that there is nucleic acid gel with liquid crystal structure very big fracture to stretch
Long rate.
Embodiment 4
Take double dodecyls two of salmon DNA (2000 base-pairs) solution and 3mL 50mM of 600 μ L 10mg/ml
Methyl bromide ammonium is put into centrifuge tube, and concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in 10000rpm
Centrifugal speed under be centrifuged 30 minutes, remove supernatant, be added deionized water washing after be centrifuged, wash to move back three times repeatedly and remove
Centrifugation gained complex precipitate is put into liquid nitrogen and freezes, places into freeze-drying instrument and be lyophilized two hours by supernatant.Then freezing
The dimethyl sulfoxide of 50 μ L is added in sample after dry, places 30 minutes at room temperature, obtains nucleic acid gel, obtain core through wire drawing
Acid cure glue fiber.Fig. 2 is the ductility test chart of nucleic acid gel manufactured in the present embodiment, and resulting nucleic acid gel fiber can prolong
It opens up to 330 times of length, illustrates that nucleic acid gel of the invention has excellent ductility.
Embodiment 5
Take double dodecyls two of salmon DNA (2000 base-pairs) solution and 4mL 50mM of 800 μ L 10mg/ml
Methyl bromide ammonium is put into centrifuge tube, and concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in 10000rpm
Centrifugal speed under be centrifuged 30 minutes, remove supernatant, be added deionized water washing after be centrifuged, wash to move back three times repeatedly and remove
Centrifugation gained complex precipitate is put into liquid nitrogen and freezes, places into freeze-drying instrument and be lyophilized two hours by supernatant.Then freezing
The tetrahydrofuran of 80 μ L is added in sample after dry, places 30 minutes at room temperature, obtains nucleic acid gel fiber through wire drawing.Fig. 3
For the mechanical property of nucleic acid gel fiber manufactured in the present embodiment, calculate resulting Young's modulus and toughness be respectively 32MPa and
18MJ/m3.Illustrate nucleic acid gel fiber of the invention much higher than other reported nucleic acid soft material systems, toughness can match in excellence or beauty
The natural spider's thread.
Embodiment 6
Take the cetyl trimethyl of salmon DNA (2000 base-pairs) solution and 5mL 50mM of 1mL 10mg/ml
Ammonium bromide is put into centrifuge tube, and concussion stirring 1 minute at white suspension.Be then placed in centrifuge, 10000rpm from
It is centrifuged 30 minutes under heart speed, removes supernatant, after deionized water washing precipitating is added, be centrifuged again, after washing three times repeatedly
Supernatant is removed, centrifugation gained precipitating is lyophilized, is put into freeze-drying instrument overnight.Then 60 μ L are added in sample after freeze drying
Ethyl alcohol, place 30 minutes at room temperature, obtain nucleic acid gel, manual wire drawing obtains nucleic acid gel fiber of the present invention.Through detecting,
The nucleic acid gel has liquid crystal structure, carries out Mechanics Performance Testing to nucleic acid gel fiber obtained and ductility is tested, as a result
It is close with 2~3 result of embodiment, no significant difference (p > 0.05)
Embodiment 7
Take the DNA solution of the 2686bp (2686 base-pairs) of 8mL 5mg/ml and the didecyldimethylammonium of 4mL 50mM
Ammonium bromide is put into centrifuge tube, and concussion stirring 1 minute at white suspension.Be then placed in centrifuge, 10000rpm from
It is centrifuged 30 minutes under heart speed, removes supernatant, be centrifuged after deionized water washing is added, wash and move back three times except supernatant repeatedly
Centrifugation gained complex precipitate is put into liquid nitrogen and freezes, places into freeze-drying instrument and be lyophilized two hours by liquid.Then after freeze drying
Sample in the dimethyl sulfoxide of 50 μ L is added, place 30 minutes at room temperature, obtain nucleic acid gel, manual wire drawing obtains this hair
Bright nucleic acid gel fiber.Through detecting, which has liquid crystal structure, carries out mechanical property to nucleic acid gel fiber obtained
It can test and ductility test, as a result close with 2~3 result of embodiment, no significant difference (p > 0.05)
Comparative example 1
Take the DNA solution of 2000 base-pairs of 100 μ L5mM and single dodecyl trimethyl ammonium bromide of 5mL 50mM
It is put into centrifuge tube, concussion stirring 1 minute at white suspension.It is then placed in centrifuge, in the centrifugal speed of 10000rpm
Lower centrifugation 30 minutes, removes supernatant, is centrifuged after deionized water washing is added, washs and move back three times except supernatant repeatedly, will be from
Complex precipitate obtained by the heart, which is put into liquid nitrogen, to be freezed, and is placed into freeze-drying instrument and is lyophilized two hours.Then in sample after freeze drying
It is added in the water of 100 μ L, places 60 minutes at room temperature, obtain nucleic acid hydrogel, which does not have birefringent characteristic, that is, does not have
There is liquid crystal structure, while the nucleic acid hydrogel can not stretch, does not have ductility.
Comparative example 2
Take the DNA solution of 2000 base-pairs of 100 μ L5mM and the polyethylene glycols ammonium bromide of 5mL 50mM be put into from
In heart pipe, concussion stirring 1 minute at white suspension.It is then placed in centrifuge, is centrifuged under the centrifugal speed of 10000rpm
30 minutes, supernatant is removed, is centrifuged after deionized water washing is added, washs and moves back three times except supernatant repeatedly, by centrifugation gained
Complex precipitate is put into liquid nitrogen and freezes, and places into freeze-drying instrument and is lyophilized two hours.Then it is added in sample after freeze drying
In the water of 100 μ L, places 60 minutes at room temperature, obtain nucleic acid hydrogel, which does not have birefringent characteristic, i.e., no liquid
Crystal structure, while the nucleic acid hydrogel can not stretch, and not have ductility.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of nucleic acid gel fiber characterized by comprising
It takes nucleic acid to react with cationic surfactant, obtains compound；
Organic solvent is added into the compound to be swollen, nucleic acid gel is obtained；
By the nucleic acid gel wire drawing, nucleic acid gel fiber is obtained.
2. preparation method according to claim 1, which is characterized in that nucleic acid is in terms of base number, the nucleic acid and cation
The molar ratio of surfactant is 1:1~1:10.
3. preparation method according to claim 1, which is characterized in that the nucleic acid is long-chain DNA, described
Long-chain DNA contains 200~2686 base-pairs.
4. preparation method according to claim 1, which is characterized in that the nucleic acid is short chain DNA, described
Short chain DNA contains 14~22 base-pairs.
5. preparation method according to claim 1, which is characterized in that the compound is by nucleic acid and cation surface activating
Agent reacts to obtain by electrostatic interaction.
6. preparation method according to claim 1, which is characterized in that the cationic surfactant is selected from double dodecanes
Base ditallowdimethyl ammonium bromide, one of cetyl trimethylammonium bromide or didecyldimethylammonium bromide or a variety of.
7. preparation method according to claim 1, which is characterized in that the organic solvent is selected from dimethyl sulfoxide, tetrahydro
Furans, chloroform, toluene, ethyl alcohol, one of ethylene glycol or glycerine or a variety of.
8. preparation method according to claim 1, which is characterized in that the time of the swelling is 10min-2h.
9. preparation method according to claim 1, which is characterized in that it is molten that organic solvent progress is added in Xiang Suoshu compound
The step of swollen further include washing the compound centrifugation before, freeze-drying.
10. nucleic acid gel fiber made from any one of claim 1~9 preparation method.
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| CN110484990A (en) * | 2019-08-22 | 2019-11-22 | 中国科学院长春应用化学研究所 | A kind of protein binding rare earths fiber and preparation method thereof |
| CN113136628A (en) * | 2021-05-25 | 2021-07-20 | 清华大学 | Biological fiber, preparation method thereof and wet spinning device |
| CN113372902A (en) * | 2021-06-07 | 2021-09-10 | 清华大学 | DNA composite gel for optical information storage and preparation method thereof |
| CN114848504A (en) * | 2022-03-23 | 2022-08-05 | 清华大学 | DNA-hydroxyapatite composite material for manufacturing antibacterial tooth inlays and preparation method and application thereof |
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