A kind of microbial bacterial agent and its preparation method and application for handling breeding wastewater
Technical field
The present invention relates to technical field of waste water processing more particularly to a kind of microbial bacterial agent for handling breeding wastewater and
Preparation method and application.
Background technique
Aquaculture industry of China is continued to develop with the raising of people's quality of life, and raiser is more and more, and cultivation scale is got over
Come bigger.Aquaculture is always heavy polluter, is more than the nitrogen of half since livestock and poultry are poor to the protein adsorption in feed, in feed
Phosphorus substance is discharged with fecaluria, therefore contains a large amount of nitrogen phosphorus in breeding wastewater.If breeding wastewater is directly discharged into nature without processing
Water body will cause water eutrophication, and then cause a series of environmental problems such as cyanobacterial bloom and soil hardening, seriously will be straight
Connect the healthy and safe of the threat mankind.Therefore, breeding wastewater how is effectively treated as very urgent problem.
At present both at home and abroad there are three types of the Main Patterns of processing breeding wastewater: returning to the field mode, Natural treatment mode and industrialization
Tupe.Returning to the field mode, which refers to, is directly discharged into farmland for breeding wastewater, and nitrogen phosphorus improves soil as fertilizer using in breeding wastewater
Fertility, its advantage is that it is easy to operate, low in cost, but enough soils is needed to come the so many breeding wastewater of containing, and nitrogen
Phosphorus is more than after soil loads, and soil also will suffer from polluting.Natural treatment mode refers to using means such as artificial swamp and ecological ponds,
Organic matter and nitrogen phosphorus using the synergy of natural microorganisms and water plant, in degrading cultivation waste water.This method cost compared with
Low and certain pollutant that can degrade, but treatment effect is affected by environment big, when contaminant pressure or big environment influence, is
System easily collapse, encounters big rainfall, and pollution can also further be spread.Industrial treatment mode refers to, by separation of solid and liquid, anaerobism
A series of industrial treatments such as pond and aerobic tank achieve the purpose that pollution degradation, and this method treatment effect is preferable, and system is also opposite
Stablize, but builds high with maintenance cost.Currently, China's major part scale livestock farming uses industrial treatment mode, strictly press
Breeding wastewater water outlet may be implemented according to operation standard and reach national emission standard, and in real system operational process, by water inlet wave
The influence with maintenance cost is moved, organic pollutant and nitrogen phosphorus cannot still remove well in breeding wastewater.With national environmental protection political affairs
Plan is more and more severeer, and pollution reduction requires also increasingly to improve, and the farm that discharge standard is not achieved faces the risk shut down.It is logical
It crosses and adds suitable microbial bacterial agent, contaminant removal efficiency can be greatly improved, while can also effectively remove some difficult to degrade
Soluble pollutants, ensure wastewater effluent be far below national emission standard.
Denitrogenation also rests on ammonia nitrogen removal level in cultivating wastewater purification at present, and currently available technology is seldom related to going for total nitrogen
It removes, " livestock and poultry breeding industry pollutant emission standard " (the GB18596-20012003-01-01 implementation) being carrying out including China
The discharge standard of total nitrogen is not limited.After mineralized nitrogen is nitrite nitrogen and nitrate nitrogen, traditional handicraft is needed in anaerobic environment
It is just able to achieve denitrification, converts nitrogen for nitrate nitrogen, this needs raiser to add an anaerobic pond, to aggravate raiser
Burden, therefore the prior art does not often consider total nitrogen index, and the high sewage of total nitrogen concentration is discharged into natural water, can undoubtedly increase
The Nature self-purification of water burden, makes water eutrophication.
The advantage of this patent compared with prior art is, aerobic denitrifying bacteria is introduced composite bacteria agent formula, is not necessarily to anaerobism
Pond is able to achieve denitrification, removes total nitrogen while removing ammonia nitrogen.As environmental protection policy is more and more tighter, total nitrogen standard from now on one
Surely livestock and poultry breeding industry pollutant emission standard can be added, and the technology that this patent provides realizes total nitrogen emission reduction in advance.
Summary of the invention
Technical problem to be solved by the present invention lies in, provide it is a kind of for handling the microbial bacterial agent of breeding wastewater, can
The ammonia nitrogen and total nitrogen in breeding wastewater are removed simultaneously.
Technical problem to be solved by the present invention lies in provide a kind of for handling the system of the microbial bacterial agent of breeding wastewater
Preparation Method, it is possible to reduce mycoprotein frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
Technical problem to be solved by the present invention lies in provide a kind of for handling answering for the microbial bacterial agent of breeding wastewater
With microbial bacterial agent is added in breeding wastewater, the ammonia nitrogen and total nitrogen in breeding wastewater, cost for wastewater treatment can be removed simultaneously
It is low.
In order to solve the above-mentioned technical problems, the present invention provides a kind of for handling the microbial bacterial agent of breeding wastewater, wraps
Including bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, the total living bacteria count of microbial bacterial agent is greater than 2 ×
108CFU/g;
The aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
As an improvement of the above scheme, the bacillus includes bacillus subtilis, bacillus coagulans and lichens bud
Spore bacillus;
The nitrobacteria includes bacterium nitrobacter and nitrosomonas.
As an improvement of the above scheme, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and aerobic
1-5 parts of denitrifying bacteria;
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part;
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter;
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
As an improvement of the above scheme, the saccharomycete is candidiasis.
Correspondingly, the present invention also provides a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, special
Sign is, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor;
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid;
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial;
The strain includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, and the aerobic denitrification is thin
Bacterium bag includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
As an improvement of the above scheme, the protective agent includes sucrose, skimmed milk power and gelatin.
As an improvement of the above scheme, the protective agent includes 3-8 parts of sucrose, 2-6 parts of skimmed milk power and gelatin 0.5-2.5
Part.
As an improvement of the above scheme, the bacillus includes bacillus subtilis, bacillus coagulans and lichens bud
Spore bacillus;
The nitrobacteria includes bacterium nitrobacter and nitrosomonas;
The saccharomycete is candidiasis.
As an improvement of the above scheme, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and aerobic
1-5 parts of denitrifying bacteria;
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part;
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter;
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
Correspondingly, the present invention also provides a kind of for handling the application of the microbial bacterial agent of breeding wastewater, by microorganism
Microbial inoculum is added in the aerobic tank of waste water treatment system, wherein the dosage of microbial bacterial agent is 10-100mg/L, in aerobic tank
Dissolved oxygen content be greater than 2mg/L, pH 7.0-8.5, carbon-nitrogen ratio be (5-20): 1, N/P ratio be (5-20): 1, hydraulic retention
Time is 8-120h.
The invention has the following beneficial effects:
1, provided by the invention a kind of for handling the microbial bacterial agent of breeding wastewater, it applies and is led in cultivating wastewater purification
Domain, the microbial bacterial agent include bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria.The present invention passes through gemma
The mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria effectively removes COD, BOD in breeding wastewater5、
Ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A standard.
Wherein, the aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.This
The aerobic denitrifying bacteria of invention can remove ammonia nitrogen, nitre state salt and nitrous state salt in waste water in existing aerobic tank, thus
It does not need to add anaerobic pond, greatlys save cost for wastewater treatment.
2, provided by the invention a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, by different thallus
It is mixed with protective agent and carries out vacuum freeze drying, obtain solid microbial.The microbial bacterial agent prepared using the method for the present invention,
Can be in order to it store and transport, and number of viable is effectively improved, reduce cost.
Wherein, the protective agent includes sucrose, skimmed milk power and gelatin.The present invention reduces mycoprotein by protective agent
The frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
3, the present invention provides a kind of for handling the application of the microbial bacterial agent of breeding wastewater, and mentioned microorganism microbial inoculum is thrown
It is added in the aerobic tank of waste water treatment system.Aerobic denitrifying bacteria of the invention can remove in waste water in existing aerobic tank
Ammonia nitrogen, nitre state salt and nitrous state salt, without adding anaerobic pond, compared with existing anaerobic denitrifying technology, this hair
The realization more preferable than anaerobic pond of bright aerobic tank, cost is lower, is suitble to cultivating wastewater purification.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention will be made below further detailed
Description.
Provided by the invention a kind of for handling the microbial bacterial agent of breeding wastewater, the microbial bacterial agent, which is applied, to be cultivated
Field of waste water treatment.
The microbial bacterial agent includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria.Wherein, gemma
Larger molecular organics in waste water are decomposed into small organic molecule by bacillus, thus COD and ammonia nitrogen in degrading waste water, and be it
He provides carbon source at microorganism growth;Saccharomycete by carbohydrate, protide and the grease type organic in waste water resolve into carbon dioxide,
Water, small molecular organic acid and polypeptide;Nitrobacteria by waste water polypeptide and ammonium salt species be decomposed into nitrate;Aerobic denitrification
Ammonia nitrogen, nitre state salt and nitrous state salt in waste water is decomposed into nitrogen by bacterium, to remove the ammonia nitrogen and total nitrogen in waste water.
The present invention passes through the mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, effectively removes
COD, BOD in breeding wastewater5, ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A mark
It is quasi-, wherein COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 8mg/L.
In addition, aerobic denitrifying bacteria of the invention can remove ammonia nitrogen, nitre state salt in waste water in existing aerobic tank
Cost for wastewater treatment is greatlyd save without adding anaerobic pond with nitrous state salt.
In order to guarantee that treated, water outlet reaches above-mentioned standard, and the total living bacteria count of microbial bacterial agent has to be larger than 2
×108CFU/g.If the total living bacteria count of microbial bacterial agent is lower than 2 × 108CFU/g, then waste water treatment efficiency is low, or even is not achieved
Treatment effect.
Preferably, the aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
The bacillus includes bacillus subtilis, bacillus coagulans and bacillus licheniformis.The nitrobacteria includes nitrification
Bacillus and nitrosomonas.The saccharomycete is candidiasis.It should be noted that above-mentioned Pseudomonas is not limited to the application institute
State the bacterium listed.
Specifically, the microbial bacterial agent include 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria become reconciled
1-5 parts of oxygen denitrifying bacteria;
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part;
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter;
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
It should be noted that strain of the present invention can be commonly micro- from China Committee for Culture Collection of Microorganisms
Bio-Centers (CGMCC) and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center
(ACCC) it is commercially available.
It is a further object of the present invention to provide a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, including
Following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor;
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid;
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial;
The strain includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, and the aerobic denitrification is thin
Bacterium bag includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
Protective agent of the invention can change Frozen Biological it is dry when physics, chemical environment, mitigate or prevent cold
Damage of the dry or rehydration to cell is lyophilized, original various physio-biochemical characteristics and bioactivity are kept as far as possible, to reduce
Mycoprotein frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
Specifically, the protective agent includes sucrose, skimmed milk power and gelatin.Wherein, sucrose belongs to osmosis type protective agent, can
It penetrates into cell wall but cell membrane cannot be penetrated, aquation occurs osmosis type protective agent for easy bound water molecule in the solution, makes
The viscosity of solution increases, to weaken the crystallization process of water, inhibits the phase transformation of plasma membrane during microbial cell drying and dehydrating,
To achieve the purpose that protection.Skimmed milk power main component is protein, belongs to macromolecular protective agent, and macromolecular protective agent is logical
The form protection thallus of " package " is crossed, while low molecule protective agent being promoted to play a role.Gelatin belongs to impermeable type protective agent, non-
Osmosis type protective agent not can enter cell, it makes solution in supercooled state, can reduce solute concentration at a certain temperature, thus
It plays a protective role.
Existing protective agent is not suitable for the general formulation of different microorganisms type, and existing protective agent is usually multiple
Close formula.Protective agent type of the present invention and formula are formulated for microbial bacterial agent of the present invention, with different types of protective agent
Combination can increase protection efficiency, while be good excipient, can promote product appearance quality.
Preferably, the protective agent includes 3-8 parts of sucrose, 2-6 parts of skimmed milk power and 0.5-2.5 parts of gelatin.More preferably, institute
Stating protective agent includes 5 parts of sucrose, 3 parts of skimmed milk power and 1 part of gelatin.
In addition, the microbial bacterial agent prepared using the above method, it can be in order to store and transport, and effectively improve viable count
Amount, reduces cost.
It should be noted that the bacillus includes bacillus subtilis, bacillus coagulans and bacillus licheniformis;
The nitrobacteria includes bacterium nitrobacter and nitrosomonas;The saccharomycete is candidiasis.
Specifically, the microbial bacterial agent, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria become reconciled
1-5 parts of oxygen denitrifying bacteria;
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part;
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter;
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
It is a further object of the present invention to provide a kind of for handling the application of the microbial bacterial agent of breeding wastewater, by microorganism
Microbial inoculum is added in the aerobic tank of waste water treatment system, wherein the dosage of microbial bacterial agent is 10-100mg/L, in aerobic tank
Dissolved oxygen content be greater than 2mg/L, pH 7.0-8.5, carbon-nitrogen ratio be (5-20): 1, N/P ratio be (5-20): 1, hydraulic retention
Time is 8-120h.Wherein, carbon-nitrogen ratio is with (BOD5:TN) calculating, and N/P ratio is with (TN:TP) calculating.
The present invention passes through the mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, effectively removes
COD, BOD in breeding wastewater5, ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A mark
It is quasi-, wherein COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 8mg/L.
In addition, aerobic denitrifying bacteria of the invention can remove ammonia nitrogen, nitre state salt in waste water in existing aerobic tank
With nitrous state salt, without adding anaerobic pond, compared with existing anaerobic denitrifying technology, aerobic tank ratio of the invention is detested
Oxygen pond more preferably realizes that cost is lower, is suitble to cultivating wastewater purification.
It should be noted that microorganism, which plays a role, needs certain hydraulic detention time, if when aerobic tank hydraulic retention
Between be lower than 8h, then be easy to cause the loss of microorganism, system processing capacity reduces.If because of the limitation of conditions, hydraulic detention time needs
It will be in 8h hereinafter, treatment effect can be reached by way of increasing microorganism dosage.
Below the present invention will be illustrated with specific embodiment
Embodiment 1
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor;
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g;
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial;
The strain includes 5 parts of bacillus, 3 parts of saccharomycete, 4 parts of nitrobacteria and 3 parts of aerobic denitrifying bacteria;
The bacillus includes 3 parts of bacillus subtilis, 3 parts of bacillus coagulans and 3 parts of bacillus licheniformis;
The nitrobacteria includes 3 parts and 4 parts of nitrosomonas of bacterium nitrobacter;
The aerobic denitrifying bacteria includes 4 parts of pseudomonas mendocina, 3 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 3
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 100mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, and pH is
7.0-8.5, carbon-nitrogen ratio are (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 8h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
|
COD(mg/L) |
BOD5(mg/L) |
Total nitrogen (mg/L) |
Ammonia nitrogen (mg/L) |
Anaerobic pond water inlet |
1022.6 |
670.8 |
165.4 |
113.5 |
Anaerobic pond water outlet |
551.2 |
302.3 |
138.5 |
110.6 |
The water outlet of level-one aerobic tank |
315.4 |
172.3 |
37.3 |
33.9 |
The water outlet of second level aerobic tank |
47.8 |
9.3 |
13.6 |
4.7 |
Removal rate |
95.3% |
98.6% |
91.8% |
95.9% |
Embodiment 2
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor;
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g;
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial;
The strain includes 2 parts of bacillus, 2 parts of saccharomycete, 1 part of nitrobacteria and 1 part of aerobic denitrifying bacteria;
The bacillus includes 1 part of bacillus subtilis, 1 part of bacillus coagulans and 1 part of bacillus licheniformis;
The nitrobacteria includes 1 part and 2 parts of nitrosomonas of bacterium nitrobacter;
The aerobic denitrifying bacteria includes 2 parts of pseudomonas mendocina, 1 part of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 1
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 10mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, pH 7.0-
8.5, carbon-nitrogen ratio is (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 120h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
|
COD(mg/L) |
BOD5(mg/L) |
Total nitrogen (mg/L) |
Ammonia nitrogen (mg/L) |
Anaerobic pond water inlet |
770.7 |
363.8 |
114.2 |
95.8 |
Anaerobic pond water outlet |
480.2 |
211.0 |
99.5 |
93.7 |
The water outlet of level-one aerobic tank |
197.1 |
103.5 |
33.1 |
22.5 |
The water outlet of second level aerobic tank |
25.0 |
6.9 |
7.4 |
3.7 |
Removal rate |
96.8% |
98.1% |
93.5% |
96.1% |
Embodiment 3
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor;
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g;
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial;
The strain includes 3 parts of bacillus, 5 parts of saccharomycete, 6 parts of nitrobacteria and 5 parts of aerobic denitrifying bacteria;
The bacillus includes 5 parts of bacillus subtilis, 5 parts of bacillus coagulans and 4 parts of bacillus licheniformis;
The nitrobacteria includes 6 parts and 7 parts of nitrosomonas of bacterium nitrobacter;
The aerobic denitrifying bacteria includes 6 parts of pseudomonas mendocina, 4 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 6
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 60mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, pH 7.0-
8.5, carbon-nitrogen ratio is (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 72h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
|
COD(mg/L) |
BOD5(mg/L) |
Total nitrogen (mg/L) |
Ammonia nitrogen (mg/L) |
Anaerobic pond water inlet |
2248.9 |
680.7 |
167.7 |
95.0 |
Anaerobic pond water outlet |
1371.8 |
354.0 |
116.8 |
89.2 |
The water outlet of level-one aerobic tank |
157.1 |
57.2 |
45.9 |
44.4 |
The water outlet of second level aerobic tank |
31.8 |
8.5 |
9.1 |
4.8 |
Removal rate |
98.6% |
98.8% |
94.6% |
94.9% |
After embodiment 1,2 and 3 is using microbial bacterial agent of the present invention processing waste water, aerobic tank effluent quality is obviously changed
Kind, water outlet reaches national town sewage discharge standard better than national livestock and poultry breeding industry sewage drainage standard (GB 18596-2001)
Level-one A standard (GB 18918-2002, COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 5mg/L).
Above disclosed is only a preferred embodiment of the present invention, cannot limit the power of the present invention with this certainly
Sharp range, therefore equivalent changes made in accordance with the claims of the present invention, are still within the scope of the present invention.