CN109880762A - A kind of microbial bacterial agent and its preparation method and application for handling breeding wastewater - Google Patents
A kind of microbial bacterial agent and its preparation method and application for handling breeding wastewater Download PDFInfo
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- CN109880762A CN109880762A CN201910170887.1A CN201910170887A CN109880762A CN 109880762 A CN109880762 A CN 109880762A CN 201910170887 A CN201910170887 A CN 201910170887A CN 109880762 A CN109880762 A CN 109880762A
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- 239000002351 wastewater Substances 0.000 title claims abstract description 72
- 230000000813 microbial effect Effects 0.000 title claims abstract description 67
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 59
- 238000009395 breeding Methods 0.000 title claims abstract description 54
- 230000001488 breeding effect Effects 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 60
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 60
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 53
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 36
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 29
- 241000589755 Pseudomonas mendocina Species 0.000 claims abstract description 17
- 241000589614 Pseudomonas stutzeri Species 0.000 claims abstract description 17
- 239000003223 protective agent Substances 0.000 claims description 28
- 235000015097 nutrients Nutrition 0.000 claims description 18
- 241000193749 Bacillus coagulans Species 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 241000605122 Nitrosomonas Species 0.000 claims description 15
- 229940054340 bacillus coagulans Drugs 0.000 claims description 15
- 238000004065 wastewater treatment Methods 0.000 claims description 15
- 241000605154 Nitrobacter winogradskyi Species 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 108010010803 Gelatin Proteins 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229920000159 gelatin Polymers 0.000 claims description 9
- 239000008273 gelatin Substances 0.000 claims description 9
- 235000019322 gelatine Nutrition 0.000 claims description 9
- 235000011852 gelatine desserts Nutrition 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 235000020183 skimmed milk Nutrition 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 7
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 7
- 238000010564 aerobic fermentation Methods 0.000 claims description 6
- 241000222122 Candida albicans Species 0.000 claims description 5
- 206010007134 Candida infections Diseases 0.000 claims description 5
- 201000003984 candidiasis Diseases 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 28
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 24
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 21
- 239000010865 sewage Substances 0.000 abstract description 7
- -1 making that treated Substances 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 description 10
- 150000003839 salts Chemical class 0.000 description 10
- 238000011282 treatment Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 230000006872 improvement Effects 0.000 description 7
- 239000002068 microbial inoculum Substances 0.000 description 6
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 5
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 239000003513 alkali Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 208000001034 Frostbite Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010059410 Faecaluria Diseases 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002595 cold damage Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C12R2001/05—Alcaligenes
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- C12R2001/72—Candida
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a kind of for handling the microbial bacterial agent of breeding wastewater, including bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, and the total living bacteria count of microbial bacterial agent is greater than 2 × 108CFU/g；The aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.Correspondingly, the present invention also provides a kind of for handling the preparation method and application of the microbial bacterial agent of breeding wastewater.The present invention passes through the mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, effectively removes COD, BOD in breeding wastewater5, ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A standard.
Description
Technical field
The present invention relates to technical field of waste water processing more particularly to a kind of microbial bacterial agent for handling breeding wastewater and
Preparation method and application.
Background technique
Aquaculture industry of China is continued to develop with the raising of people's quality of life, and raiser is more and more, and cultivation scale is got over
Come bigger.Aquaculture is always heavy polluter, is more than the nitrogen of half since livestock and poultry are poor to the protein adsorption in feed, in feed
Phosphorus substance is discharged with fecaluria, therefore contains a large amount of nitrogen phosphorus in breeding wastewater.If breeding wastewater is directly discharged into nature without processing
Water body will cause water eutrophication, and then cause a series of environmental problems such as cyanobacterial bloom and soil hardening, seriously will be straight
Connect the healthy and safe of the threat mankind.Therefore, breeding wastewater how is effectively treated as very urgent problem.
At present both at home and abroad there are three types of the Main Patterns of processing breeding wastewater: returning to the field mode, Natural treatment mode and industrialization
Tupe.Returning to the field mode, which refers to, is directly discharged into farmland for breeding wastewater, and nitrogen phosphorus improves soil as fertilizer using in breeding wastewater
Fertility, its advantage is that it is easy to operate, low in cost, but enough soils is needed to come the so many breeding wastewater of containing, and nitrogen
Phosphorus is more than after soil loads, and soil also will suffer from polluting.Natural treatment mode refers to using means such as artificial swamp and ecological ponds,
Organic matter and nitrogen phosphorus using the synergy of natural microorganisms and water plant, in degrading cultivation waste water.This method cost compared with
Low and certain pollutant that can degrade, but treatment effect is affected by environment big, when contaminant pressure or big environment influence, is
System easily collapse, encounters big rainfall, and pollution can also further be spread.Industrial treatment mode refers to, by separation of solid and liquid, anaerobism
A series of industrial treatments such as pond and aerobic tank achieve the purpose that pollution degradation, and this method treatment effect is preferable, and system is also opposite
Stablize, but builds high with maintenance cost.Currently, China's major part scale livestock farming uses industrial treatment mode, strictly press
Breeding wastewater water outlet may be implemented according to operation standard and reach national emission standard, and in real system operational process, by water inlet wave
The influence with maintenance cost is moved, organic pollutant and nitrogen phosphorus cannot still remove well in breeding wastewater.With national environmental protection political affairs
Plan is more and more severeer, and pollution reduction requires also increasingly to improve, and the farm that discharge standard is not achieved faces the risk shut down.It is logical
It crosses and adds suitable microbial bacterial agent, contaminant removal efficiency can be greatly improved, while can also effectively remove some difficult to degrade
Soluble pollutants, ensure wastewater effluent be far below national emission standard.
Denitrogenation also rests on ammonia nitrogen removal level in cultivating wastewater purification at present, and currently available technology is seldom related to going for total nitrogen
It removes, " livestock and poultry breeding industry pollutant emission standard " (the GB18596-20012003-01-01 implementation) being carrying out including China
The discharge standard of total nitrogen is not limited.After mineralized nitrogen is nitrite nitrogen and nitrate nitrogen, traditional handicraft is needed in anaerobic environment
It is just able to achieve denitrification, converts nitrogen for nitrate nitrogen, this needs raiser to add an anaerobic pond, to aggravate raiser
Burden, therefore the prior art does not often consider total nitrogen index, and the high sewage of total nitrogen concentration is discharged into natural water, can undoubtedly increase
The Nature self-purification of water burden, makes water eutrophication.
The advantage of this patent compared with prior art is, aerobic denitrifying bacteria is introduced composite bacteria agent formula, is not necessarily to anaerobism
Pond is able to achieve denitrification, removes total nitrogen while removing ammonia nitrogen.As environmental protection policy is more and more tighter, total nitrogen standard from now on one
Surely livestock and poultry breeding industry pollutant emission standard can be added, and the technology that this patent provides realizes total nitrogen emission reduction in advance.
Summary of the invention
Technical problem to be solved by the present invention lies in, provide it is a kind of for handling the microbial bacterial agent of breeding wastewater, can
The ammonia nitrogen and total nitrogen in breeding wastewater are removed simultaneously.
Technical problem to be solved by the present invention lies in provide a kind of for handling the system of the microbial bacterial agent of breeding wastewater
Preparation Method, it is possible to reduce mycoprotein frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
Technical problem to be solved by the present invention lies in provide a kind of for handling answering for the microbial bacterial agent of breeding wastewater
With microbial bacterial agent is added in breeding wastewater, the ammonia nitrogen and total nitrogen in breeding wastewater, cost for wastewater treatment can be removed simultaneously
It is low.
In order to solve the above-mentioned technical problems, the present invention provides a kind of for handling the microbial bacterial agent of breeding wastewater, wraps
Including bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, the total living bacteria count of microbial bacterial agent is greater than 2 ×
108CFU/g；
The aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
As an improvement of the above scheme, the bacillus includes bacillus subtilis, bacillus coagulans and lichens bud
Spore bacillus；
The nitrobacteria includes bacterium nitrobacter and nitrosomonas.
As an improvement of the above scheme, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and aerobic
1-5 parts of denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
As an improvement of the above scheme, the saccharomycete is candidiasis.
Correspondingly, the present invention also provides a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, special
Sign is, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial；
The strain includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, and the aerobic denitrification is thin
Bacterium bag includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
As an improvement of the above scheme, the protective agent includes sucrose, skimmed milk power and gelatin.
As an improvement of the above scheme, the protective agent includes 3-8 parts of sucrose, 2-6 parts of skimmed milk power and gelatin 0.5-2.5
Part.
As an improvement of the above scheme, the bacillus includes bacillus subtilis, bacillus coagulans and lichens bud
Spore bacillus；
The nitrobacteria includes bacterium nitrobacter and nitrosomonas；
The saccharomycete is candidiasis.
As an improvement of the above scheme, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and aerobic
1-5 parts of denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
Correspondingly, the present invention also provides a kind of for handling the application of the microbial bacterial agent of breeding wastewater, by microorganism
Microbial inoculum is added in the aerobic tank of waste water treatment system, wherein the dosage of microbial bacterial agent is 10-100mg/L, in aerobic tank
Dissolved oxygen content be greater than 2mg/L, pH 7.0-8.5, carbon-nitrogen ratio be (5-20): 1, N/P ratio be (5-20): 1, hydraulic retention
Time is 8-120h.
The invention has the following beneficial effects:
1, provided by the invention a kind of for handling the microbial bacterial agent of breeding wastewater, it applies and is led in cultivating wastewater purification
Domain, the microbial bacterial agent include bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria.The present invention passes through gemma
The mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria effectively removes COD, BOD in breeding wastewater5、
Ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A standard.
Wherein, the aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.This
The aerobic denitrifying bacteria of invention can remove ammonia nitrogen, nitre state salt and nitrous state salt in waste water in existing aerobic tank, thus
It does not need to add anaerobic pond, greatlys save cost for wastewater treatment.
2, provided by the invention a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, by different thallus
It is mixed with protective agent and carries out vacuum freeze drying, obtain solid microbial.The microbial bacterial agent prepared using the method for the present invention,
Can be in order to it store and transport, and number of viable is effectively improved, reduce cost.
Wherein, the protective agent includes sucrose, skimmed milk power and gelatin.The present invention reduces mycoprotein by protective agent
The frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
3, the present invention provides a kind of for handling the application of the microbial bacterial agent of breeding wastewater, and mentioned microorganism microbial inoculum is thrown
It is added in the aerobic tank of waste water treatment system.Aerobic denitrifying bacteria of the invention can remove in waste water in existing aerobic tank
Ammonia nitrogen, nitre state salt and nitrous state salt, without adding anaerobic pond, compared with existing anaerobic denitrifying technology, this hair
The realization more preferable than anaerobic pond of bright aerobic tank, cost is lower, is suitble to cultivating wastewater purification.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention will be made below further detailed
Description.
Provided by the invention a kind of for handling the microbial bacterial agent of breeding wastewater, the microbial bacterial agent, which is applied, to be cultivated
Field of waste water treatment.
The microbial bacterial agent includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria.Wherein, gemma
Larger molecular organics in waste water are decomposed into small organic molecule by bacillus, thus COD and ammonia nitrogen in degrading waste water, and be it
He provides carbon source at microorganism growth；Saccharomycete by carbohydrate, protide and the grease type organic in waste water resolve into carbon dioxide,
Water, small molecular organic acid and polypeptide；Nitrobacteria by waste water polypeptide and ammonium salt species be decomposed into nitrate；Aerobic denitrification
Ammonia nitrogen, nitre state salt and nitrous state salt in waste water is decomposed into nitrogen by bacterium, to remove the ammonia nitrogen and total nitrogen in waste water.
The present invention passes through the mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, effectively removes
COD, BOD in breeding wastewater5, ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A mark
It is quasi-, wherein COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 8mg/L.
In addition, aerobic denitrifying bacteria of the invention can remove ammonia nitrogen, nitre state salt in waste water in existing aerobic tank
Cost for wastewater treatment is greatlyd save without adding anaerobic pond with nitrous state salt.
In order to guarantee that treated, water outlet reaches above-mentioned standard, and the total living bacteria count of microbial bacterial agent has to be larger than 2
×108CFU/g.If the total living bacteria count of microbial bacterial agent is lower than 2 × 108CFU/g, then waste water treatment efficiency is low, or even is not achieved
Treatment effect.
Preferably, the aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
The bacillus includes bacillus subtilis, bacillus coagulans and bacillus licheniformis.The nitrobacteria includes nitrification
Bacillus and nitrosomonas.The saccharomycete is candidiasis.It should be noted that above-mentioned Pseudomonas is not limited to the application institute
State the bacterium listed.
Specifically, the microbial bacterial agent include 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria become reconciled
1-5 parts of oxygen denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
It should be noted that strain of the present invention can be commonly micro- from China Committee for Culture Collection of Microorganisms
Bio-Centers (CGMCC) and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center
(ACCC) it is commercially available.
It is a further object of the present invention to provide a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, including
Following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial；
The strain includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, and the aerobic denitrification is thin
Bacterium bag includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
Protective agent of the invention can change Frozen Biological it is dry when physics, chemical environment, mitigate or prevent cold
Damage of the dry or rehydration to cell is lyophilized, original various physio-biochemical characteristics and bioactivity are kept as far as possible, to reduce
Mycoprotein frostbite death rate in refrigerating process improves the number of viable of microbial inoculum after freezing.
Specifically, the protective agent includes sucrose, skimmed milk power and gelatin.Wherein, sucrose belongs to osmosis type protective agent, can
It penetrates into cell wall but cell membrane cannot be penetrated, aquation occurs osmosis type protective agent for easy bound water molecule in the solution, makes
The viscosity of solution increases, to weaken the crystallization process of water, inhibits the phase transformation of plasma membrane during microbial cell drying and dehydrating,
To achieve the purpose that protection.Skimmed milk power main component is protein, belongs to macromolecular protective agent, and macromolecular protective agent is logical
The form protection thallus of " package " is crossed, while low molecule protective agent being promoted to play a role.Gelatin belongs to impermeable type protective agent, non-
Osmosis type protective agent not can enter cell, it makes solution in supercooled state, can reduce solute concentration at a certain temperature, thus
It plays a protective role.
Existing protective agent is not suitable for the general formulation of different microorganisms type, and existing protective agent is usually multiple
Close formula.Protective agent type of the present invention and formula are formulated for microbial bacterial agent of the present invention, with different types of protective agent
Combination can increase protection efficiency, while be good excipient, can promote product appearance quality.
Preferably, the protective agent includes 3-8 parts of sucrose, 2-6 parts of skimmed milk power and 0.5-2.5 parts of gelatin.More preferably, institute
Stating protective agent includes 5 parts of sucrose, 3 parts of skimmed milk power and 1 part of gelatin.
In addition, the microbial bacterial agent prepared using the above method, it can be in order to store and transport, and effectively improve viable count
Amount, reduces cost.
It should be noted that the bacillus includes bacillus subtilis, bacillus coagulans and bacillus licheniformis；
The nitrobacteria includes bacterium nitrobacter and nitrosomonas；The saccharomycete is candidiasis.
Specifically, the microbial bacterial agent, including 1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria become reconciled
1-5 parts of oxygen denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and bacillus licheniformis 1-4
Part；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes that 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and excrement produce alkali bar
1-5 parts of bacterium.
It is a further object of the present invention to provide a kind of for handling the application of the microbial bacterial agent of breeding wastewater, by microorganism
Microbial inoculum is added in the aerobic tank of waste water treatment system, wherein the dosage of microbial bacterial agent is 10-100mg/L, in aerobic tank
Dissolved oxygen content be greater than 2mg/L, pH 7.0-8.5, carbon-nitrogen ratio be (5-20): 1, N/P ratio be (5-20): 1, hydraulic retention
Time is 8-120h.Wherein, carbon-nitrogen ratio is with (BOD5:TN) calculating, and N/P ratio is with (TN:TP) calculating.
The present invention passes through the mutual cooperation of bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, effectively removes
COD, BOD in breeding wastewater5, ammonia nitrogen and total nitrogen, making that treated, water outlet reaches national town sewage discharge standard level-one A mark
It is quasi-, wherein COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 8mg/L.
In addition, aerobic denitrifying bacteria of the invention can remove ammonia nitrogen, nitre state salt in waste water in existing aerobic tank
With nitrous state salt, without adding anaerobic pond, compared with existing anaerobic denitrifying technology, aerobic tank ratio of the invention is detested
Oxygen pond more preferably realizes that cost is lower, is suitble to cultivating wastewater purification.
It should be noted that microorganism, which plays a role, needs certain hydraulic detention time, if when aerobic tank hydraulic retention
Between be lower than 8h, then be easy to cause the loss of microorganism, system processing capacity reduces.If because of the limitation of conditions, hydraulic detention time needs
It will be in 8h hereinafter, treatment effect can be reached by way of increasing microorganism dosage.
Below the present invention will be illustrated with specific embodiment
Embodiment 1
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial；
The strain includes 5 parts of bacillus, 3 parts of saccharomycete, 4 parts of nitrobacteria and 3 parts of aerobic denitrifying bacteria；
The bacillus includes 3 parts of bacillus subtilis, 3 parts of bacillus coagulans and 3 parts of bacillus licheniformis；
The nitrobacteria includes 3 parts and 4 parts of nitrosomonas of bacterium nitrobacter；
The aerobic denitrifying bacteria includes 4 parts of pseudomonas mendocina, 3 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 3
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 100mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, and pH is
7.0-8.5, carbon-nitrogen ratio are (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 8h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
| COD(mg/L) | BOD5(mg/L) | Total nitrogen (mg/L) | Ammonia nitrogen (mg/L) | |
| Anaerobic pond water inlet | 1022.6 | 670.8 | 165.4 | 113.5 |
| Anaerobic pond water outlet | 551.2 | 302.3 | 138.5 | 110.6 |
| The water outlet of level-one aerobic tank | 315.4 | 172.3 | 37.3 | 33.9 |
| The water outlet of second level aerobic tank | 47.8 | 9.3 | 13.6 | 4.7 |
| Removal rate | 95.3% | 98.6% | 91.8% | 95.9% |
Embodiment 2
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial；
The strain includes 2 parts of bacillus, 2 parts of saccharomycete, 1 part of nitrobacteria and 1 part of aerobic denitrifying bacteria；
The bacillus includes 1 part of bacillus subtilis, 1 part of bacillus coagulans and 1 part of bacillus licheniformis；
The nitrobacteria includes 1 part and 2 parts of nitrosomonas of bacterium nitrobacter；
The aerobic denitrifying bacteria includes 2 parts of pseudomonas mendocina, 1 part of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 1
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 10mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, pH 7.0-
8.5, carbon-nitrogen ratio is (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 120h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
| COD(mg/L) | BOD5(mg/L) | Total nitrogen (mg/L) | Ammonia nitrogen (mg/L) | |
| Anaerobic pond water inlet | 770.7 | 363.8 | 114.2 | 95.8 |
| Anaerobic pond water outlet | 480.2 | 211.0 | 99.5 | 93.7 |
| The water outlet of level-one aerobic tank | 197.1 | 103.5 | 33.1 | 22.5 |
| The water outlet of second level aerobic tank | 25.0 | 6.9 | 7.4 | 3.7 |
| Removal rate | 96.8% | 98.1% | 93.5% | 96.1% |
Embodiment 3
It is a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, comprising the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately list
For colony inoculation in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, is sent out
Zymotic fluid, wherein living bacteria count is greater than 2 × 10 in fermentation liquid9CFU/g；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, by different thallus and protective agent
Mixing carries out vacuum freeze drying, obtains solid microbial；
The strain includes 3 parts of bacillus, 5 parts of saccharomycete, 6 parts of nitrobacteria and 5 parts of aerobic denitrifying bacteria；
The bacillus includes 5 parts of bacillus subtilis, 5 parts of bacillus coagulans and 4 parts of bacillus licheniformis；
The nitrobacteria includes 6 parts and 7 parts of nitrosomonas of bacterium nitrobacter；
The aerobic denitrifying bacteria includes 6 parts of pseudomonas mendocina, 4 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 6
Part.
It is a kind of for handling the application of the microbial bacterial agent of breeding wastewater, microbial bacterial agent is added to waste water treatment system
Aerobic tank, wherein the dosage of microbial bacterial agent is 60mg/L, and the dissolved oxygen content of aerobic tank is greater than 2mg/L, pH 7.0-
8.5, carbon-nitrogen ratio is (5-20): 1, N/P ratio is (5-20): 1, hydraulic detention time 72h.
Waste water treatment system (anaerobic-aerobic-aerobic process) carries out the waste water in each pond after continuous operation one month
It checks, as a result as follows:
| COD(mg/L) | BOD5(mg/L) | Total nitrogen (mg/L) | Ammonia nitrogen (mg/L) | |
| Anaerobic pond water inlet | 2248.9 | 680.7 | 167.7 | 95.0 |
| Anaerobic pond water outlet | 1371.8 | 354.0 | 116.8 | 89.2 |
| The water outlet of level-one aerobic tank | 157.1 | 57.2 | 45.9 | 44.4 |
| The water outlet of second level aerobic tank | 31.8 | 8.5 | 9.1 | 4.8 |
| Removal rate | 98.6% | 98.8% | 94.6% | 94.9% |
After embodiment 1,2 and 3 is using microbial bacterial agent of the present invention processing waste water, aerobic tank effluent quality is obviously changed
Kind, water outlet reaches national town sewage discharge standard better than national livestock and poultry breeding industry sewage drainage standard (GB 18596-2001)
Level-one A standard (GB 18918-2002, COD < 50mg/L, BOD5< 10mg/L, total nitrogen < 15mg/L, ammonia nitrogen < 5mg/L).
Above disclosed is only a preferred embodiment of the present invention, cannot limit the power of the present invention with this certainly
Sharp range, therefore equivalent changes made in accordance with the claims of the present invention, are still within the scope of the present invention.
Claims (10)
1. a kind of for handling the microbial bacterial agent of breeding wastewater, which is characterized in that thin including bacillus, saccharomycete, nitrification
Bacterium and aerobic denitrifying bacteria, the total living bacteria count of microbial bacterial agent are greater than 2 × 108CFU/g；
The aerobic denitrifying bacteria includes pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
2. as described in claim 1 for handling the microbial bacterial agent of breeding wastewater, which is characterized in that the bacillus packet
Include bacillus subtilis, bacillus coagulans and bacillus licheniformis；
The nitrobacteria includes bacterium nitrobacter and nitrosomonas.
3. as claimed in claim 2 for handling the microbial bacterial agent of breeding wastewater, which is characterized in that including bacillus 1-
6 parts, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and 1-5 parts of aerobic denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and 1-4 parts of bacillus licheniformis；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 1-
5 parts.
4. as described in claim 1 for handling the microbial bacterial agent of breeding wastewater, which is characterized in that the saccharomycete is false
Silk saccharomycete.
5. a kind of for handling the preparation method of the microbial bacterial agent of breeding wastewater, which comprises the following steps:
(11) it will remain in the different strain in refrigerator to activate on nutrient broth solid medium respectively, pick them separately single colonie
It is inoculated in the container of the different fluid nutrient mediums containing nutrient broth, culture obtains seed liquor；
(12) the resulting each seed liquor of step (11) is inoculated into fermentor respectively, carries out aerobic fermentation, obtains fermentation liquid；
(13) the resulting each fermentation liquid of step (12) is centrifuged, obtains thallus, different thallus and protective agent are mixed
Vacuum freeze drying is carried out, solid microbial is obtained；
The strain includes bacillus, saccharomycete, nitrobacteria and aerobic denitrifying bacteria, the aerobic denitrifying bacteria packet
Include pseudomonas mendocina, Pseudomonas stutzeri and Bacillus foecalis alkaligenes.
6. as claimed in claim 5 for handling the preparation method of the microbial bacterial agent of breeding wastewater, which is characterized in that described
Protective agent includes sucrose, skimmed milk power and gelatin.
7. as claimed in claim 5 for handling the preparation method of the microbial bacterial agent of breeding wastewater, which is characterized in that described
Protective agent includes 3-8 parts of sucrose, 2-6 parts of skimmed milk power and 0.5-2.5 parts of gelatin.
8. as claimed in claim 5 for handling the preparation method of the microbial bacterial agent of breeding wastewater, which is characterized in that described
Bacillus includes bacillus subtilis, bacillus coagulans and bacillus licheniformis；
The nitrobacteria includes bacterium nitrobacter and nitrosomonas；
The saccharomycete is candidiasis.
9. as claimed in claim 8 for handling the preparation method of the microbial bacterial agent of breeding wastewater, which is characterized in that including
1-6 parts of bacillus, 1-6 parts of saccharomycete, 1-5 parts of nitrobacteria and 1-5 parts of aerobic denitrifying bacteria；
The bacillus includes 1-5 parts of bacillus subtilis, 1-5 parts of bacillus coagulans and 1-4 parts of bacillus licheniformis；
The nitrobacteria includes 1-6 parts and nitrosomonas 2-7 parts of bacterium nitrobacter；
The aerobic denitrifying bacteria includes 2-6 parts of pseudomonas mendocina, 1-4 parts of Pseudomonas stutzeri and Bacillus foecalis alkaligenes 1-
5 parts.
10. claim 1-4 is described in any item for handling any one of microbial bacterial agent or claim 5-8 of breeding wastewater
Application of the microbial bacterial agent in breeding wastewater for handling breeding wastewater of the method preparation, which is characterized in that will be micro-
Bacteria agent is added in the aerobic tank of waste water treatment system, wherein the dosage of microbial bacterial agent is 10-100mg/L, aerobic
Dissolved oxygen content in pond is greater than 2mg/L, pH 7.0-8.5, and carbon-nitrogen ratio is (5-20): 1, N/P ratio is (5-20): and 1, waterpower
Residence time is 8-120h.
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| CN110759607B (en) * | 2019-11-29 | 2022-07-29 | 浙江三点水环保工程有限公司 | Process for removing total nitrogen from printing and dyeing wastewater |
| CN111748509A (en) * | 2020-07-03 | 2020-10-09 | 浙江工商大学 | Microbial purifying preparation for seawater culture tail water, and preparation method and application thereof |
| WO2024113537A1 (en) * | 2022-11-28 | 2024-06-06 | 麦王环境技术有限公司 | Heterotrophic nitrification-aerobic denitrification composite microbial agent, preparation method therefor, and use thereof |
| CN115820495A (en) * | 2022-12-05 | 2023-03-21 | 中国科学院天津工业生物技术研究所 | Single microbial agent and application thereof in treatment of heparin sodium wastewater |
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