CN109880738B - Positive pressure holding device and small bioreactor using same - Google Patents
Positive pressure holding device and small bioreactor using same Download PDFInfo
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- CN109880738B CN109880738B CN201711283854.5A CN201711283854A CN109880738B CN 109880738 B CN109880738 B CN 109880738B CN 201711283854 A CN201711283854 A CN 201711283854A CN 109880738 B CN109880738 B CN 109880738B
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Abstract
The invention provides a positive pressure holding device and a small bioreactor applying the same, which comprise a bioreactor tank body, a positive pressure holding device, a clamping mechanism and a third one-way conduction device, wherein the positive pressure holding device is fixed on a tank cover of the bioreactor tank body through the clamping mechanism, the third one-way conduction device is arranged on the tank cover of the bioreactor tank body, the positive pressure holding device comprises a mounting seat, a positive pressure device main body, an air treatment part and an auxiliary air supply part, the output end of the positive pressure device main body is connected with the input end of the air treatment part, and the output end of the air treatment part is connected with the input end of the auxiliary air supply part. By adopting the device, the positive pressure state of the inner cavity of the tank body can be maintained by supplementing clean air to the tank body in the moving process of the small bioreactor tank body after pulse high-temperature sterilization and disinfection is finished, so that the phenomenon that the inner cavity of the tank body forms negative pressure due to cooling of the tank body, and the subsequent projects such as biological cell culture and the like are influenced by the inhaled polluted air is effectively avoided.
Description
Technical Field
The invention relates to the technical field of positive pressure holding devices for bioreactors, in particular to a positive pressure holding device and a small bioreactor applying the same.
Background
In the prior art, in order to ensure good progress of projects such as biological cell culture, a small bioreactor tank body is often required to be subjected to airtight test and pulse high-temperature sterilization and disinfection, the sealing performance of the small bioreactor can be ensured in the airtight test, however, after the high-temperature sterilization treatment, the temperature of the small bioreactor tank body is high, the connection part is subjected to thermal expansion due to high temperature, the tank body is cooled when the small bioreactor tank body moves in a room-temperature environment, the connection part is subjected to cold contraction and becomes loose, and the inner cavity of the tank body generates negative pressure at the moment, so that external air is easily pressed into the inner cavity of the tank body from the loose part, the air is subjected to treatment such as filtration and sterilization without being subjected to, and particles such as bacterial dust and the like contained in the tank body often cause bacterial breeding when the subsequent cell culture is carried out in the inner cavity.
Disclosure of Invention
The invention mainly aims to solve the problems in the prior art, and provides a positive pressure holding device which has a simple structure and is convenient and quick to install, and can maintain the positive pressure state of an inner cavity of a tank body of a small bioreactor by supplementing clean air to the tank body in the moving process after the tank body completes pulsation high-temperature sterilization and disinfection, so that the phenomenon that the inner cavity of the tank body forms negative pressure due to cooling of the tank body, and polluted air is sucked to influence subsequent projects such as biological cell culture and the like is effectively avoided, and the small bioreactor using the positive pressure holding device.
In order to solve the technical problems, the invention adopts the technical scheme that: a positive pressure holding device comprises a mounting seat, a positive pressure device main body, an air processing part and an auxiliary air supply part, wherein the positive pressure device main body is arranged on the upper surface of the mounting seat, the output end of the positive pressure device main body is connected with the input end of the air processing part, and the output end of the air processing part is connected with the input end of the auxiliary air supply part;
the positive pressure device main body comprises a shell, a controller, a power supply unit, a motor driver and a micro vacuum pump, wherein the controller is fixed on the inner wall surface of the shell, the power supply unit, the motor driver and the micro vacuum pump are arranged in the inner cavity of the shell, the power supply unit and the motor driver are both electrically connected with the controller, the power supply unit is electrically connected with the motor driver, the motor driver is electrically connected with the micro vacuum pump, the air outlet of the micro vacuum pump is connected with the input end of the air treatment part, and the air inlet of the micro vacuum pump is communicated with the air inlet on the shell;
the power supply unit is a rechargeable battery pack with a power supply of 12V-36V.
Further, the auxiliary air supply part is arranged to comprise an air storage cavity and a one-way conduction device I, the air storage cavity is rotatably arranged at the lower end of the air treatment part, the top wall of the air storage cavity is communicated with the output end of the air treatment part through a one-way conduction device II, the one-way conduction device I is arranged on the bottom wall of the air storage cavity, and the bottom of the one-way conduction device I is fixedly connected with the connector in a coaxial mode.
Further, one-way conduction device I and two-way conduction device II all set up to include through-hole, base, elastic element and ball stifled, the through-hole is connected gradually by hole section I, changeover portion, hole section II and hole section III and is constituteed, the diameter of hole section I sets up to be less than the diameter of ball stifled, hole section II all sets up to be greater than the diameter of ball stifled with the diameter of hole section three, base interference fit coaxial line sets up in hole section three, and the coaxial line in this base sets up the water conservancy diversion through-hole, elastic element's one end is fixed in the upper surface of base, and this elastic element's the other end is fixed in the lower extreme of ball stifled.
Further, the air treatment part is set to include an air filter and an ultraviolet sterilization device, a second air inlet of the air filter is communicated with a first air outlet of the micro vacuum pump through a silica gel air pipe, a second air outlet of the air filter is communicated with the ultraviolet sterilization device in a rotating mode, and an auxiliary air supply part is arranged below the ultraviolet sterilization device.
Furthermore, the ultraviolet sterilization device comprises a sterilization device shell, a first cover body and a second cover body, the first cover body and the second cover body are matched and clamped on two sides of the sterilization device shell, a reflection layer is arranged on the inner wall surface of the sterilization device shell, ultraviolet lamps are vertically fixed at the centers of the inner surfaces of the first cover body and the second cover body, quartz sleeves are vertically arranged on the inner surfaces of the first cover body and the second cover body, the quartz sleeves are arranged on the upper side and the lower side of the ultraviolet lamps, and the quartz sleeves on the first cover body and the second cover body are arranged in a staggered mode.
Further, still set up the air outlet on the casing, the coaxial line sets up the air pipe joint in the air outlet.
Further, the filter membrane pore size of the air filter is set to be less than 0.22 μm.
A small bioreactor applying a positive pressure holding device comprises a bioreactor tank body, a positive pressure holding device, a clamping mechanism and a one-way conduction device III, wherein the positive pressure holding device is fixed on a tank cover of the bioreactor tank body through the clamping mechanism, the clamping mechanism is fixed on a mounting seat of the positive pressure holding device, and the one-way conduction device III is arranged on the tank cover of the bioreactor tank body.
Further, clamping mechanism sets up to include two supporting seats, guide bar, screw rod, two sliders and two clamping parts, two on the corresponding vertical fixation of supporting seat is on the mount pad, two adjacent and parallel arrangement guide bar and screw rod between the supporting seat, corresponding two sections external screw threads that revolve to opposite are set up on the screw rod, and revolves respectively on the external screw thread section of this screw rod and set up the slider, the one end of slider still overlaps on locating the guide bar, and the other end integrated into one piece of this slider sets up clamping part, clamping part's internal face sets up to be the arc.
Further, the structure of the one-way conduction device III is the same as that of the one-way conduction device I.
The invention has the advantages and positive effects that:
(1) external air is sucked through the micro vacuum pump of the positive pressure device main body, obtained gas is subjected to filtering sterilization through the air treatment part and then is matched with the auxiliary air supply part to be supplemented into the inner cavity of the bioreactor tank body, so that the problem that subsequent projects such as biological cell culture and the like are influenced due to negative pressure formed by poor sealing in the moving and cooling process of a room temperature environment after the small bioreactor is subjected to pulsating high-temperature sterilization and disinfection is effectively solved, and the rechargeable battery pack can provide electric energy for the micro vacuum pump to ensure good work of the micro vacuum pump.
(2) Through the gas storage chamber, the cooperation of one-way conduction device one and one-way conduction device two, can store in the gas storage chamber by the clean air of micro vacuum pump income and after the filtration sterilization of air treatment part, in order to guarantee to cool off the removal in-process at small-size bioreactor, and when malleation device main part cooperateed with the air treatment part and not obtained clean air yet, the clean air can in time be mended into to the inner chamber of small-size bioreactor in the gas storage chamber, thereby maintain the pressure of jar internal chamber, avoid producing the negative pressure and inhale contaminated air.
(3) Through the structure of through-hole, the cooperation of elastic element and ball plug, can push ball plug compression elastic element when the atmospheric pressure that is close to a hole section one side is higher than the atmospheric pressure of a hole section three one sides for the air gets into the low pressure side by the high pressure side, and when the atmospheric pressure that is close to three sides of a hole section is higher than the atmospheric pressure of a hole section one side, promotes ball plug and compresses tightly in the changeover portion, with the circulation that stops the air, thereby realize the one-way good circulation of air in the gas storage chamber.
(4) Can carry out good filtering to the dust particle etc. that contain in the air through the air cleaner, and ultraviolet germicidal device then can further carry out sterilization to the air to it is clean to effectively guarantee to get into the gas in the bioreactor jar body, avoids the cell culture process failure that leads to because of air pollution, so that causes cost loss's problem.
(5) Through clamping mechanism, can make malleation holding device convenient and fast ground install on the bioreactor jar body, and effectively avoid the bioreactor jar body temperature after high temperature sterilization handles high, easily scald staff's problem during the installation, and the setting of one-way conduction device three, then can in time carry out the discharge of the unnecessary air of bioreactor jar body inner chamber to maintain the pressure of its inner chamber, guarantee the normal work of the bioreactor jar body.
Drawings
Fig. 1 is a schematic view of the positive pressure maintaining apparatus according to the present invention.
FIG. 2 is a schematic view showing a structure in which the air treatment part and the auxiliary air supply part of FIG. 1 are applied to a small-sized bioreactor.
Fig. 3 is a partially enlarged schematic view of fig. 1.
Fig. 4 is a schematic structural view of the positive pressure device main body and the clamping mechanism.
Fig. 5 is a left side view of the structure of fig. 4.
Fig. 6 is a schematic right-side view of fig. 4, which shows a sectional structure of the positive pressure apparatus main body.
FIG. 7 is a schematic view of a partial cross-sectional view of a tank lid of the bioreactor tank of FIG. 2.
Fig. 8 is a block diagram of the circuit configuration of the main body of the positive pressure apparatus.
In the figure: the device comprises a mounting seat 10, a positive pressure device main body 20, a shell 201, a controller 202, a power supply unit 203, a motor driver 204, a micro vacuum pump 205, a first air outlet 206, a first air inlet 207, an air processing part 30, an air filter 301, a shell 302, a first cover body 303, a second cover body 304, an ultraviolet lamp 305, a quartz sleeve 306, an auxiliary air supply part 40, an air storage cavity 401, a first one-way conduction device 402, a second one-way conduction device 403, a base 4031, an elastic element 4032, a ball plug 4033, a first hole section 4034, a transition section 4035, a second hole section 4036, a third hole section 4037, a bioreactor tank 50, a clamping mechanism 60, a supporting seat 601, a guide rod 602, a screw 603, a sliding block 604, a clamping part 605 and a third one-way conduction.
Detailed Description
For a better understanding of the present invention, reference is made to the following detailed description and accompanying drawings that illustrate the invention.
As shown in fig. 1 to 8, a positive pressure holding device comprises a mounting seat 10, a positive pressure device main body 20, an air processing part 30 and an auxiliary air supply part 40, wherein the positive pressure device main body 20 is arranged on the upper surface of the mounting seat 10, the output end of the positive pressure device main body 20 is connected with the input end of the air processing part 30, and the output end of the air processing part 30 is connected with the input end of the auxiliary air supply part 40.
The positive pressure device main body 20 is configured to include a housing 201, a controller 202, a power supply unit 203, a motor driver 204 and a micro vacuum pump 205, the controller 202 is fixed on an inner wall surface of the housing 201, the power supply unit 203, the motor driver 204 and the micro vacuum pump 205 are arranged in an inner cavity of the housing 201, the power supply unit 203 and the motor driver 204 are both electrically connected to the controller 202, the power supply unit 203 is electrically connected to the motor driver 204, the motor driver 204 is electrically connected to the micro vacuum pump 205, an air outlet one 206 of the micro vacuum pump 205 is connected to an input end of the air processing portion 30, an air inlet one 207 of the micro vacuum pump 205 is communicated with an air inlet on the housing 201, an air supply rate of the micro vacuum pump 205 is set to be greater than 20ml/min, and preferably, the air.
The power supply unit 203 is a rechargeable battery pack with a power supply of 12V-36V, external air is sucked through the micro vacuum pump 205 of the positive pressure device main body 20, obtained gas is filtered and sterilized through the air treatment part 30 and then is matched with the auxiliary air supply part 40 to be supplemented into the inner cavity of the bioreactor tank body 50, so that the problem that the subsequent cell culture is influenced due to the fact that negative pressure is formed due to poor sealing in the moving and cooling process of a room temperature environment after the small bioreactor is subjected to pulse high-temperature sterilization is effectively avoided, and the rechargeable battery pack can provide electric energy for the micro vacuum pump 205 to guarantee good work of the micro vacuum pump 205.
The auxiliary air supply part 40 comprises an air storage cavity 401 and a one-way conduction device I402, the air storage cavity 401 is arranged at the lower end of the air treatment part 30 in a rotating mode, the top wall of the air storage cavity 401 is communicated with the output end of the air treatment part 30 through a one-way conduction device II 403, the one-way conduction device I402 is arranged on the bottom wall of the air storage cavity 401, the bottom of the one-way conduction device I402 is fixedly connected with a joint in a coaxial mode, clean air pumped by the micro vacuum pump 205 and filtered and sterilized by the air treatment part 30 can be stored in the air storage cavity 401 through the matching of the air storage cavity 401, the one-way conduction device I402 and the one-way conduction device II 403, and therefore when the small bioreactor is cooled and moved and the positive pressure device main body 20 is matched with the air treatment part 30 and clean air is not obtained yet, the air can be timely supplemented into the inner cavity of, thereby maintaining the pressure of the inner cavity of the tank body and avoiding generating negative pressure to suck polluted air.
The first unidirectional conduction device 402 and the second unidirectional conduction device 403 are respectively provided with a through hole, a base 4031, an elastic element 4032 and a ball plug 4033, the through hole is formed by sequentially connecting a first hole section 4034, a transition section 4035, a second hole section 4036 and a third hole section 4037, the diameter of the first hole section 4034 is smaller than that of the ball plug 4033, the diameters of the second hole section 4036 and the third hole section 4037 are respectively larger than that of the ball plug 4033, the base 4031 is in interference fit with the common axis in the third hole section 4037, a flow guide through hole is formed in the common axis in the base 4031, the base 4031 is fixed on the bottom wall of the gas storage cavity 401 or the sterilization device shell 302, one end of the elastic element 4032 is fixed on the upper surface of the base 4031, the other end of the elastic element 4032 is fixed on the lower end of the ball plug 4033, the second hole section 4036 is provided in a circular truncated cone shape, the diameter of the second hole section 4036 is smaller than that of the third hole section 4037, and the structure of the through, when the air pressure on the side close to the first hole section 4034 is higher than the air pressure on the side close to the third hole section 4037, the push ball plug 4033 can be pushed to compress the elastic element 4032, so that air enters the low-pressure side from the high-pressure side, and when the air pressure on the side close to the third hole section 4037 is higher than the air pressure on the side close to the first hole section 4034, the push ball plug 4033 is pushed to be pressed in the transition section 4035, so that the circulation of the air is prevented, and therefore the unidirectional good circulation of the air in the air storage cavity 401 is realized.
The air treatment part 30 is arranged to comprise an air filter 301 and an ultraviolet sterilization device, a second air inlet of the air filter 301 is communicated with a first air outlet 206 of the micro vacuum pump 205 through a silica gel air pipe, the second air outlet of the air filter 301 is matched and communicated with the ultraviolet sterilization device in a rotating mode, the auxiliary air supply part 40 is arranged below the ultraviolet sterilization device, dust particles and the like contained in the air can be well filtered through the air filter 301, the ultraviolet sterilization device can further sterilize the air, so that the air entering the bioreactor tank body 50 is effectively ensured to be clean, failure of a cell culture process caused by air pollution is avoided, and cost loss is caused.
The ultraviolet sterilization device comprises a sterilization device shell 302, a first cover body 303 and a second cover body 304, wherein the first cover body 303 and the second cover body 304 are clamped at two sides of the sterilization device shell 302 in a matching manner, a reflecting layer is arranged on the inner wall surface of the sterilization device shell 302, an ultraviolet lamp 305 is vertically fixed at the centers of the inner surfaces of the first cover body 303 and the second cover body 304, quartz sleeves 306 are vertically arranged on the inner surfaces of the first cover body 303 and the second cover body 304, the quartz sleeves 306 are arranged at the upper side and the lower side of the ultraviolet lamp 305, the quartz sleeves 306 on the first cover body 303 and the second cover body 304 are staggered with each other, in order to protect the lamp tube from being impacted and ensure the projection rate of ultraviolet C light, in order to protect the lamp tube from being impacted, quartz materials with the wall thickness of 1.5mm are adopted as the sleeves, the light transmittance can reach more than 90%, the sterilization device shell 302, the first cover body 303, can make the air that gets into sterilizing equipment casing 302 inner chamber carry out ultraviolet sterilization and handle, the setting of reflection stratum can improve ultraviolet utilization ratio, and the quartz sleeve 306 of phase-to-phase staggering setting has then prolonged the bactericidal action of air in sterilizing equipment casing 302 for a long time to further guarantee the good sterilization to the air and handle.
The shell 201 is also provided with an air outlet, an air pipe joint is arranged on the coaxial line of the air outlet, the inner diameter of the air pipe joint is set to be 5mm, the section of the outer diameter of the air pipe joint is set to be trapezoidal, the outer diameter of the air pipe joint close to one side of the shell 201 is set to be 10mm, the outer diameter of the air pipe joint far away from one side of the shell 201 is set to be 9mm,
the filter membrane pore size of the air filter 301 is set to be less than 0.22 μm.
A small bioreactor applying a positive pressure holding device comprises a bioreactor tank body 50, a positive pressure holding device, a clamping mechanism 60 and a one-way conduction device III 70, wherein the positive pressure holding device is fixed on a tank cover of the bioreactor tank body 50 through the clamping mechanism 60, and a clamping mechanism 60 is fixed on the mounting seat 10 of the positive pressure holding device, a one-way conduction device III 70 is arranged on the tank cover of the bioreactor tank body 50, the positive pressure holding device can be conveniently and quickly mounted on the bioreactor tank 50 by the clamping mechanism 60, and effectively avoids the problem that the bioreactor tank body 50 after high-temperature sterilization treatment is difficult to install due to high temperature, and the arrangement of the one-way conduction device III 70 can discharge the redundant air in the inner cavity of the bioreactor tank body 50 in time so as to maintain the pressure of the inner cavity and ensure the normal work of the bioreactor tank body 50.
The clamping mechanism 60 comprises two supporting seats 601, a guide rod 602, a screw 603, two sliding blocks 604 and two clamping parts 605, wherein the two supporting seats 601 are correspondingly and vertically fixed on the mounting seat 10, the guide rod 602 and the screw 603 are adjacently and parallelly arranged between the two supporting seats 601, two sections of external threads with opposite rotation directions are correspondingly arranged on the screw 603, the sliding blocks 604 are respectively and spirally arranged on the external thread sections of the screw 603, one end of each sliding block 604 is also sleeved on the guide rod 602, the clamping part 605 is integrally formed at the other end of each sliding block 604, the inner wall surface of each clamping part 605 is arc-shaped, the two sliding blocks 604 are enabled to move oppositely on the screw 603 by manually rotating the screw 603 and matching with the guide rod 602, so that the clamping parts 605 are close to or far away from each other, the positive pressure holding device is ensured to be well and conveniently mounted on the bioreactor tank 50, and meanwhile, the structure of the clamping mechanism 60 can meet the clamping on the tank covers of various bioreactor tanks 50, making it more adaptable.
The configuration of the third unidirectional conducting device 70 is set to be the same as the configuration of the first unidirectional conducting device 402.
The positive pressure holding device and the small bioreactor applying the same provided by the invention have the advantages of simple structure and convenience in installation, and can maintain the positive pressure state of the inner cavity of the tank body by supplementing clean air to the tank body in the moving process of the tank body of the small bioreactor after finishing pulse high-temperature sterilization and disinfection, so that the phenomenon that the inner cavity of the tank body forms negative pressure due to cooling of the tank body, and the subsequent projects such as biological cell culture and the like are influenced by the suction of polluted air is effectively avoided. The positive pressure holding device and the installation process thereof on the small bioreactor are as follows:
1. ultraviolet lamps 305 are vertically fixed in the centers of the inner surfaces of the first cover body 303 and the second cover body 304, quartz sleeves 306 are arranged on the upper side and the lower side of the ultraviolet lamps 305 in a staggered mode, a reflecting layer is arranged on the inner wall surface of the sterilizing device shell 302, then the first cover body 303 and the second cover body 304 are respectively clamped on the two sides of the sterilizing device shell 302, the connecting position of the two cover bodies is sealed through a sealing rubber ring, a plurality of sets of one-way conducting devices 403 are arranged on the bottom wall of the sterilizing device shell 302, finally, a gas storage cavity 401 is fixedly screwed on the bottom wall of the sterilizing device shell 302 through the sealing rubber ring, and a one-way conducting device 402 is arranged on the bottom wall of the gas;
2. an air outlet II of the air filter 301 is connected with the ultraviolet sterilization device in a rotating mode, an air inlet I207 of the micro vacuum pump 205 is communicated with an air inlet on the shell 201, an air inlet II of the air filter 301 is communicated with an air outlet I206 of the micro vacuum pump 205 through a silica gel air pipe, the ultraviolet sterilization device and the air storage cavity 401 are vacuumized through a vacuumizing machine (not shown in the figure), then the controller 202 starts the power supply unit 203, the motor driver 204 and the micro vacuum pump 205 to work, so that the micro vacuum pump 205 sucks external air, the external air is stored in the air storage cavity 401 after being processed through the subsequent air filter 301 and the ultraviolet sterilization device until the air pressure in the air storage cavity 401 is equal to or slightly less than the atmospheric pressure (a pressure sensor is arranged in the air storage cavity 401 and electrically connected with the controller, and the pressure condition in the air storage cavity 401 can be observed in real time through, turning off the power supply unit 203, the motor driver 204 and the micro vacuum pump 205;
3. after the bioreactor tank 50 is subjected to pulse high-temperature sterilization treatment, the connecting joint coaxially fixed at the bottom of the one-way conduction device I402 is screwed on the tank cover of the bioreactor tank 50, then the screw 603 is manually rotated, and the two sliding blocks 604 move oppositely on the screw 603 under the cooperation of the guide rod 602, so that the clamping parts 605 are mutually close to each other, the positive pressure holding device is ensured to be well and conveniently mounted on the bioreactor tank 50, and the positive pressure holding device and the mounting process of the positive pressure holding device on a small bioreactor are completed.
The positive pressure adjusting process of the positive pressure maintaining device to the small bioreactor is as follows:
1. firstly, because it takes a period of time for the clean air treated by the micro vacuum pump 205 and the air treatment part 30 to enter the bioreactor tank 50, the clean air stored in the gas storage cavity 401 automatically supplements the gas to the bioreactor tank 50 in a negative pressure state before assembly, in the process, because the pressure in the gas storage cavity 401 is higher than that of the bioreactor tank 50, the gas in the inner cavity pushes the ball plug 4033 to compress the elastic element 4032, the through hole is opened, so that the air enters the low pressure side from the high pressure side, and the gas is supplemented to the bioreactor tank 50, so that the untreated air is prevented from being directly sucked from the connecting gap by the negative pressure formed in the inner cavity, and the positive pressure maintaining device is ensured to maintain the good working performance of the positive pressure in the inner cavity of the tank;
2. when the gas storage cavity 401 assists in supplementing gas, the controller 202 starts the power supply unit 203, the motor driver 204 and the micro vacuum pump 205 to work, so that the micro vacuum pump 205 sucks external air, and the external air is supplied to the gas storage cavity 401 after being processed by the subsequent air filter 301 and the ultraviolet sterilization device, so as to ensure positive pressure compensation for the inner cavity of the bioreactor tank body 50;
3. after the clean air in the air storage cavity 401 enters the bioreactor tank 50, when the pressure in the inner cavity of the bioreactor tank 50 is equal to or higher than the external pressure, the ball plug 4033 is pushed to compress the elastic element 4032, the through hole is opened, so that the air enters the low-pressure side from the high-pressure side through the flow guide through hole, the pressure in the inner cavity of the tank is maintained in a proper range, the normal operation of the bioreactor tank 50 is ensured, in order to further avoid that the external air possibly enters the inner cavity of the tank when the three one-way conduction device 70 works, a device with the same structure as the air treatment part 30 is communicated with an air outlet of the three one-way conduction device 70, namely, the three one-way conduction device 70 is connected with the ultraviolet sterilization device through a connecting joint, the ultraviolet sterilization device is communicated with an air filter, so that the pressure in the inner cavity of the small bioreactor tank is further maintained well, and the, so as to effectively avoid the influence of the inhaled polluted air on the subsequent engineering of biological cell culture and the like.
The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention should be covered by the present patent.
Claims (7)
1. A positive pressure retention device characterized by: the positive pressure maintaining device comprises a mounting seat, a positive pressure device main body, an air processing part and an auxiliary air supply part, wherein the positive pressure device main body is arranged on the upper surface of the mounting seat, the output end of the positive pressure device main body is connected with the input end of the air processing part, and the output end of the air processing part is connected with the input end of the auxiliary air supply part;
the positive pressure device main body comprises a shell, a controller, a power supply unit, a motor driver and a micro vacuum pump, wherein the controller is fixed on the inner wall surface of the shell, the power supply unit, the motor driver and the micro vacuum pump are arranged in the inner cavity of the shell, the power supply unit and the motor driver are both electrically connected with the controller, the power supply unit is electrically connected with the motor driver, the motor driver is electrically connected with the micro vacuum pump, the air outlet of the micro vacuum pump is connected with the input end of the air treatment part, and the air inlet of the micro vacuum pump is communicated with the air inlet on the shell;
the power supply unit is a rechargeable battery pack with a power supply of 12V-36V;
the auxiliary air supply part comprises an air storage cavity and a one-way conduction device I, the air storage cavity is spirally arranged at the lower end of the air processing part, the top wall of the air storage cavity is communicated with the output end of the air processing part through a one-way conduction device II, the one-way conduction device I is arranged on the bottom wall of the air storage cavity, and the bottom of the one-way conduction device I is fixedly connected with the connector along the same axis;
the one-way conduction device I and the one-way conduction device II are both arranged to comprise a through hole, a base, an elastic element and a ball plug, the through hole is formed by sequentially connecting a hole section I, a transition section, a hole section II and a hole section III, the diameter of the hole section I is smaller than that of the ball plug, the diameters of the hole section II and the hole section III are larger than that of the ball plug, the base is arranged in the hole section III in an interference fit coaxial line, a flow guide through hole is formed in the coaxial line in the base, one end of the elastic element is fixed on the upper surface of the base, and the other end of the elastic element is fixed at the lower end of the ball plug;
the air treatment part sets up to including air cleaner and ultraviolet germicidal device, air filter's two gas outlets that pass through silica gel trachea intercommunication micro vacuum pump of air cleaner are one, and this air filter's two gas outlets join in marriage the intercommunication ultraviolet germicidal device soon, ultraviolet germicidal device's below sets up supplementary air feed part.
2. The positive pressure retention device according to claim 1, wherein: the ultraviolet sterilization device comprises a sterilization device shell, a first cover body and a second cover body, wherein the first cover body and the second cover body are clamped on two sides of the sterilization device shell in a matched mode, a reflection layer is arranged on the inner wall face of the sterilization device shell, an ultraviolet lamp is vertically fixed at the center of the inner surface of the first cover body and the center of the inner surface of the second cover body, quartz sleeves are vertically arranged on the inner surfaces of the first cover body and the second cover body, the quartz sleeves are arranged on the upper side and the lower side of the ultraviolet lamp, and the quartz sleeves on the first cover body and the second cover body are arranged in.
3. The positive pressure retention device according to claim 1, wherein: still set up the air outlet on the casing, the coaxial line sets up the air pipe connector in the air outlet.
4. The positive pressure retention device according to claim 1, wherein: the filter membrane pore size of the air filter is set to be less than 0.22 micron.
5. A small-sized bioreactor to which the positive pressure maintaining apparatus according to any one of claims 1 to 4 is applied, characterized in that: the small bioreactor applying the positive pressure holding device comprises a bioreactor tank body, a positive pressure holding device, a clamping mechanism and a one-way conduction device III, wherein the positive pressure holding device is fixed on a tank cover of the bioreactor tank body through the clamping mechanism, the clamping mechanism is fixed on a mounting seat of the positive pressure holding device, and the one-way conduction device III is arranged on the tank cover of the bioreactor tank body.
6. The small-sized bioreactor using the positive pressure maintaining apparatus according to claim 5, wherein: clamping mechanism sets up to including two supporting seat, guide bar, screw rod, two sliders and two clamping parts, two on the corresponding vertical fixation of supporting seat is in the mount pad, two adjacent and parallel arrangement guide bar and screw rod between the supporting seat, correspond on the screw rod and set up two sections external screw threads opposite to soon, and set up the slider on the external screw thread section of this screw rod respectively soon, the one end of slider still overlaps on locating the guide bar, and the other end integrated into one piece of this slider sets up clamping part, clamping part's internal face sets up to be the arc.
7. The small-sized bioreactor using the positive pressure maintaining apparatus according to claim 5, wherein: the structure of the one-way conduction device III is the same as that of the one-way conduction device I.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711283854.5A CN109880738B (en) | 2017-12-06 | 2017-12-06 | Positive pressure holding device and small bioreactor using same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711283854.5A CN109880738B (en) | 2017-12-06 | 2017-12-06 | Positive pressure holding device and small bioreactor using same |
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| CN116036323B (en) * | 2023-04-03 | 2023-06-13 | 常州赛乐医疗技术有限公司 | Backflow preventing device of pressure steam sterilizer |
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| DE69937024T2 (en) * | 1998-07-06 | 2008-05-29 | Kubota Corp. | Process for methane fermentation of organic waste |
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| CN101249270A (en) * | 2008-03-28 | 2008-08-27 | 余兆丰 | Steam sterilizing system and sterilizing and cooling method thereof |
| CN202830018U (en) * | 2012-09-10 | 2013-03-27 | 阜阳市德益农业科技有限公司 | Liquid fungus ferment tank ventilation device |
| JP6036322B2 (en) * | 2013-01-18 | 2016-11-30 | 株式会社島津製作所 | Motor drive device and vacuum pump |
| CN107176648A (en) * | 2017-06-16 | 2017-09-19 | 苏州博菡环保科技有限公司 | A kind of photocatalyst purifier |
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