CN109874917A - A kind of triangular bream fish meal-less feed containing algal polysaccharides - Google Patents
A kind of triangular bream fish meal-less feed containing algal polysaccharides Download PDFInfo
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- CN109874917A CN109874917A CN201910237526.4A CN201910237526A CN109874917A CN 109874917 A CN109874917 A CN 109874917A CN 201910237526 A CN201910237526 A CN 201910237526A CN 109874917 A CN109874917 A CN 109874917A
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- algal polysaccharides
- triangular bream
- meal
- feed
- fish meal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
A kind of triangular bream fish meal-less feed containing algal polysaccharides, the feed comprise the following raw materials by weight percent: dregs of beans 30%, rapeseed meal 20%, peanut meal 12%, meat meal tankage 4%, brewer's yeast 4%, spy two powder 19.37-20.37%, soya-bean oil 4%, choline chloride (50%) 1%, vitamine supplement 1.5%, mineral additive 1%, calcium dihydrogen phosphate 2%, trimethylamine oxide 0.1%, phytase 0.03% and algal polysaccharides 0-1%；The present invention provides a kind of triangular bream fish meal-less feed containing algal polysaccharides, improves the immunity of triangular bream, promotes its growth, and survival rate significantly improves.
Description
Technical field
The present invention relates to feed formula technology fields, and in particular to a kind of triangular bream fish meal-less feed containing algal polysaccharides.
Background technique
Algal polysaccharides refer to one kind mainly from seaweed such as kelp, deer's tail dish (sargassum fusifome), bulk kelp, yellow tang, bladder-wracks
Multi-component mixture.Algal polysaccharide, 4 major class of red seaweed polysaccharide, green algae polysaccharide and blue algae polysaccharide can be divided into regard to its source.Polysaccharide
It is the important component of all living organisms, and in control cell division, the growth of adjusting cell and sustains life organism just
Often metabolism etc. plays a significant role.Algal polysaccharides can make up the alginate polymer of negatively charged macromolecule,
Can effectively Rich in Trace Element, many biological organisms with special physiological function can be formed, it is irritating to intestinal mucosa
Effect promotes viable organism metabolic activity, adjusts immune function, research shows that algal polysaccharides are in immunological regulation, anti-oxidant, antibacterial
Antiviral and reducing blood lipid etc. has certain effect.
Triangular bream (Megalobrama terminalis) is commonly called as " triangle bream ", " Qiantang River tower bream " etc., is subordinate to bony fish
Guiding principle, Cyprinidae, triangular bream category are a kind of relatively large economic freshwater fishes.Triangular bream body pork is thick, spur is less, meat is soft, nutrition
It is abundant, it is deep to be liked by the majority of consumers.In recent years, with the continuous depth of the researchs such as triangular bream artificial breeding, rearing of fingerling
Enter, the cultivation scale of triangular bream is also going from strength to strength, and development prospect is wide.The present invention provides a kind of triangular bream containing algal polysaccharides
Fish meal-less feed, the immunity of Lai Tigao triangular bream, to improve its survival rate.
Summary of the invention
(1) the technical issues of solving
The present invention provides a kind of triangular bream fish meal-less feed containing algal polysaccharides, improves the immunity of triangular bream, promotes it
Growth, and survival rate significantly improves.
(2) technical solution used
The present invention to achieve the above object, is achieved by the following technical programs: a kind of triangular bream containing algal polysaccharides
Fish meal-less feed, the feed comprise the following raw materials by weight percent: dregs of beans 30%, rapeseed meal 20%, peanut meal 12%, meat
Bone meal 4%, brewer's yeast 4%, two powder 19.37-20.37% of spy, soya-bean oil 4%, choline chloride (50%) 1%, vitamin addition
Agent 1.5%, mineral additive 1%, calcium dihydrogen phosphate 2%, trimethylamine oxide 0.1%, phytase 0.03% and algal polysaccharides
0-1%.
As a further optimization of the solution, the feed the preparation method comprises the following steps: weighing the raw material powder of above-mentioned per distribution ratio
It is sieved after broken through 40 mesh, is put into blender and is uniformly mixed, adding water to be mixed 10 minutes later, finally by squash type particle
Slow sinking pellet diet is made in machine, then dries the feed of preparation in the interior of drying, ventilation, is put into spare in 4 DEG C of refrigerators.
(3) beneficial effect
The present invention provides a kind of triangular bream fish meal-less feed containing algal polysaccharides, has the advantages that
Inventive formulation is scientific and reasonable, it is simple to prepare, can significantly promote triangular bream by adding algal polysaccharides in feed
Growth and improve survival rate, improve triangular bream growth performance and immunity of organisms, have in prior art breakthrough
Innovation.
Detailed description of the invention
The present invention that the following is further explained with reference to the attached drawings:
Fig. 1 is influence of the present invention to triangular bream specific growth rate；
Fig. 2 is influence of the present invention to triangular bream SOD in serum, AKP and LSZ；
Fig. 3 is the present invention on the active influence of triangular bream liver pancreas SOD；
Fig. 4 is the present invention on the active influence of triangular bream liver pancreas CAT；
Fig. 5 is influence of the present invention to triangular bream liver pancreas MDA content；
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated,
It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment
A kind of triangular bream fish meal-less feed containing algal polysaccharides, the feed comprise the following raw materials by weight percent:
Dregs of beans 30%, rapeseed meal 20%, peanut meal 12%, meat meal tankage 4%, brewer's yeast 4%, spy two powder 19.37-20.37%, soya-bean oil
4%, choline chloride (50%) 1%, vitamine supplement 1.5%, mineral additive 1%, calcium dihydrogen phosphate 2%, oxidation three
Methylamine 0.1%, phytase 0.03% and algal polysaccharides 0-1%.
As a further optimization of the solution, the feed the preparation method comprises the following steps: weighing the raw material powder of above-mentioned per distribution ratio
It is sieved after broken through 40 mesh, is put into blender and is uniformly mixed, adding water to be mixed 10 minutes later, finally by squash type particle
Slow sinking pellet diet is made in machine, then dries the feed of preparation in the interior of drying, ventilation, is put into spare in 4 DEG C of refrigerators.
Experimentation:
Culture experiment of the present invention carries out in aquatic products research institute, Hangzhou academy of agricultural sciences pond net box (1.0*1.0*1.0m), altogether
6 groups, it is randomly divided into 3 groups, every group of 3 repetitions, each 15 tail fish of repetition, 3 throwings of being satiated with food of daily 8:30,13:00,16:30 timing
It raises.27.52 ± 2.13 DEG C of water temperature during cultivation, water body dissolved oxygen are greater than 7.5mg/L, and the test period is 8 weeks.
After culture experiment, stops raising for 24 hours, record total weight and the mantissa of each net cage test fish.Then each net cage
5 tail fishes measurement body length, weight, liver pancreas weight, internal organ weight, intraabdominal adipose tissue total amount are taken at random；Each net cage takes 8 tails at random
Fish will take upper serum spare, then dissected after taking it to extract blood after blood refrigerated centrifuge (3500rpm) 10min, point
From liver pancreas, liver pancreatic tissues are weighed, fat and content are rejected on ice pan, are homogenized with 10 times of physiological saline, by tissue
Suspension refrigerated centrifuge (3000r/min), supernatant is thick enzyme extract, spare in 4 DEG C of refrigerators.
During experiment, it is satiated with food with 6 kinds of experiment feeds (Y0, Y1, Y2, Y3, Y4 and Y5) feeds experiment fish (such as the following table 1 respectively
It is shown).
The total content of inventive formulation is 100.After being added to algal polysaccharides, it is necessary to reduce content (special two powder of special two powder
Mainly as filler, rather than nutriment), it just can guarantee entire formula or 100 in this way.
Feed consumption is recorded during test, and rate of body weight gain (BWG), specific growth rate are calculated separately according to end weight and initial weight
(SGR), the indexs such as feed coefficient (FCR), abdominal fat (IPF), survival rate (SR).
Table 1 is Y0, Y1, Y2, Y3, Y4 and Y5 proportion content tables
Note: 1) it is purchased from Hangzhou Yuhang Hai Huang feed development Co., Ltd
2) forage protein, fat and ash content are measured value.
(1) influence of the algal polysaccharides to triangular bream growth performance and body index is added in fish meal-less feed；
Influence of the algal polysaccharides to triangular bream growth performance and feed conversion is added in 2 fish meal-less feed of table
As shown in Table 2, when the pitch-based sphere of algal polysaccharides is between 0.00% (YO) to 0.8% (Y4), triangular bream end
The trend gradually risen, the end of 0.2% (Y1) and 0.00% (YO) (control group) group is presented in counterpoise, rate of body weight gain and specific growth rate
There was no significant difference between counterpoise, rate of body weight gain and specific growth rate (p > 0.05), and 0.6% (Y3) -1.0% (Y5) pitch-based sphere is each
There was no significant difference between group (p>0.05), is but significantly higher than other each groups (p<0.05).The pitch-based sphere of algal polysaccharides exists
When between 0.00% (YO) to 0.8% (Y4), triangular bream feed coefficient is gradually reduced, while substantially less than Y0 (control group),
There was no significant difference between 0.2% (Y1) and 0.4% (Y2) (p > 0.05), 0.6% (Y3) -1.0% (Y5) pitch-based sphere each group
Between also there was no significant difference (p > 0.05).The display of this test result, adds the triangular bream egg of the algal polysaccharides of 0.80% (Y4)
White matter efficiency is significantly higher than other each group effects (p < 0.05).Compared with the control group triangular bream survival rate significantly improves, addition
There was no significant difference between group (p > 0.05).
Influence of the algal polysaccharides to triangular bream body index is added in 3 fish meal-less feed of table
From table 3 it is observed that the triangular bream liver body when algal polysaccharides pitch-based sphere is 0.6% (Y3) -1.0% (Y5)
Than, when abdominal fat is substantially less than control group to dirty body, coefficient of condition is significantly higher than control group (p < 0.05).
(2) algal polysaccharides are added in fish meal-less feed to triangular bream serum non-specific immunity and the anti-oxidant energy of liver pancreas
The influence of power；
(1) influence of the algal polysaccharides to triangular bream serum non-specific immunity is added in fish meal-less feed:
This test selects SOD, AKP and LSZ as triangular bream serum non-specific immunity index, and SOD is in vivo with 02-For
The enzyme of only substrate and the blocking agent of free radical chain reactions, it can specifically be catalyzed superoxide radical and be disproportionated into
Hydrogen oxide and oxygen；AKP is the important metabolic regulation enzyme of the intracorporal one kind of biology, the directly transfer of participation phosphate group and calcium phosphorus generation
It thanks, is played an important role during absorption and utilization of the fish to nutriment, in addition, AKP can be by changing cause of disease
The surface texture of body and enhance the identification by invasion body to pathogen and phagocytic activity, help to improve the premunition of fish.It is molten
Bacterium enzyme is one of the nospecific immunity factor important in organism, it results from neutrophil cell and macrophage, and by
It is secreted into performance bacteriolyze effect in blood and mucus, has stronger killing effect to gram-positive bacteria, in the ginseng of complement and antibody
Also there is killing effect to gramnegative bacterium with lower, research shows that body lysozyme activity improves, immunity is also accordingly mentioned
It is high.
As shown in Figure 1, when algal polysaccharides pitch-based sphere is 0.6%-1.0%, triangular bream specific growth rate is high；
As shown in Figure 2, addition algal polysaccharides Vee formation triangular bream serum activity of SOD increases, and 0.8% group is significantly higher than pair
According to group (p < 0.05).In this test, when algal polysaccharides pitch-based sphere is higher than 0.6%, serum AKP level activity is added with algal polysaccharides
Level steps up, and is significantly higher than control group (p < 0.05).When algal polysaccharides pitch-based sphere is higher than 0.2%, serum LSZ is living
Property be significantly higher than control group and 0.2% (Y1) group, there was no significant difference (p > 0.05) with 1.0% (Y5) group for 0.8% (Y4) group.
(2) influence of the algal polysaccharides to triangular bream liver pancreas Antioxidant Indexes is added in fish meal-less feed
For animal body during metabolism, it is flat that the generation and elimination of active oxygen radical remain a kind of dynamic
Weighing apparatus, excessive free radical can cause peroxidatic reaction of lipid, and malonaldehyde (MDA) is the main component of lipid peroxide, is had
Very strong bio-toxicity can destroy the structure and function of cell, superoxide dismutase (SOD) and catalase (CAT) etc.
The Antioxidant Enzyme Systems of composition can remove excessive free radical, reduce the peroxide injury of lipid, it is free that SOD is catalyzed superoxides
Base is disproportionated into hydrogen peroxide and oxygen, and hydrogen peroxide generates avirulent water and oxygen under the action of CAT, therefore, detects animal
The variation of SOD, CAT and MDA can relatively accurately reflect the intracorporal antioxidation status of machine.
From the figure 3, it may be seen that triangular bream liver pancreas SOD activity steps up with the raising of algal polysaccharides pitch-based sphere, and
0.4% (Y2) -1.0% (Y5) is significantly higher than control group (Y0) and 0.2% (Y2) group (p < 0.05), 0.8% (Y4) and 1.0%
(Y5) there was no significant difference between group (p > 0.05).
Figure 4, it is seen that the algal polysaccharides of different level to liver pancreas CAT activity there are different differences, work as addition
When level is greater than 0.4% (Y2) group, CAT activity is significantly higher than control group.
In Fig. 5, when algal polysaccharides pitch-based sphere is at 0.4% (Y2) -1.0% (Y5), triangular bream liver pancreas MDA content is aobvious
It writes and is lower than control group and 0.2% (Y1) group (p<0.05), but there was no significant difference each other (p>0.05).
In conclusion it is non-specific to triangular bream growth performance, body index, serum to add algal polysaccharides in triangular bream feed
Property immune and liver pancreas resistance to oxidation generate certain influence, as algal polysaccharides pitch-based sphere 0.6%-1.0%, to improving three
The growth of angle triangular bream reduces feed coefficient and strengthen immunity etc. with apparent facilitation.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (2)
1. a kind of triangular bream fish meal-less feed containing algal polysaccharides, which is characterized in that the feed is by following weight percent
Raw material composition: dregs of beans 30%, rapeseed meal 20%, peanut meal 12%, meat meal tankage 4%, brewer's yeast 4%, two powder 19.37- of spy
20.37%, soya-bean oil 4%, choline chloride (50%) 1%, vitamine supplement 1.5%, mineral additive 1%, biphosphate
Calcium 2%, trimethylamine oxide 0.1%, phytase 0.03% and algal polysaccharides 0-1%.
2. a kind of triangular bream fish meal-less feed containing algal polysaccharides according to claim 1, feature exist, the feed
The preparation method comprises the following steps: the raw material for weighing above-mentioned per distribution ratio is sieved after crushing through 40 mesh, it is put into blender and is uniformly mixed, adding later
Water is mixed 10 minutes, and slow sinking pellet diet is made finally by squash type granulator, then by the feed of preparation dry
Dry, ventilation interior is dried, and is put into spare in 4 DEG C of refrigerators.
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| CN201910237526.4A CN109874917A (en) | 2019-03-27 | 2019-03-27 | A kind of triangular bream fish meal-less feed containing algal polysaccharides |
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| CN201910237526.4A CN109874917A (en) | 2019-03-27 | 2019-03-27 | A kind of triangular bream fish meal-less feed containing algal polysaccharides |
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Citations (6)
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| CN103315140A (en) * | 2013-06-26 | 2013-09-25 | 杭州海皇饲料开发有限公司 | Triangular bream mixed feed and preparation method thereof |
| CN106306599A (en) * | 2016-08-29 | 2017-01-11 | 崇州市王场俊亨蔬菜种植专业合作社 | Aquatic animal feed |
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| CN108887532A (en) * | 2018-09-05 | 2018-11-27 | 宿松县荣学农业开发有限公司 | A kind of triangular bream feed and preparation method thereof |
| CN109198272A (en) * | 2018-10-18 | 2019-01-15 | 台州学院 | A kind of triangular bream feed formula containing yucca extract |
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2019
- 2019-03-27 CN CN201910237526.4A patent/CN109874917A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20100101742A (en) * | 2009-03-10 | 2010-09-20 | 주식회사 바이오허브 | Functional feed additives of marine fisheries and feeds for marine fisheries using thereof |
| CN103315140A (en) * | 2013-06-26 | 2013-09-25 | 杭州海皇饲料开发有限公司 | Triangular bream mixed feed and preparation method thereof |
| CN106472865A (en) * | 2015-08-13 | 2017-03-08 | 江苏苏园农业科技有限公司 | A kind of triangular bream special feed and preparation method thereof |
| CN106306599A (en) * | 2016-08-29 | 2017-01-11 | 崇州市王场俊亨蔬菜种植专业合作社 | Aquatic animal feed |
| CN108887532A (en) * | 2018-09-05 | 2018-11-27 | 宿松县荣学农业开发有限公司 | A kind of triangular bream feed and preparation method thereof |
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Application publication date: 20190614 |