CN109851841A - A kind of high resilience fibroin albumen aeroge and preparation method thereof - Google Patents
A kind of high resilience fibroin albumen aeroge and preparation method thereof Download PDFInfo
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- CN109851841A CN109851841A CN201910100161.0A CN201910100161A CN109851841A CN 109851841 A CN109851841 A CN 109851841A CN 201910100161 A CN201910100161 A CN 201910100161A CN 109851841 A CN109851841 A CN 109851841A
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Abstract
The invention discloses a kind of high resilience fibroin albumen aeroges and preparation method thereof, using natural fibroin albumen as raw material, and amides small molecule solution are added into its aqueous solution, and after high speed machine shearing foaming, freeze-drying forms aeroge.The fibroin albumen aeroge being prepared in the present invention has three-dimensional bubble structure, and inside contains a large amount of occluded airs, is not in communication with the outside, and the compression set when compression ratio is 50% is only 20% ~ 35%.Furthermore, the aeroge also has the characteristics that low-density, high porosity, good biocompatibility and degradability, it can be used for medical dressing, tissue repair etc., good biotic environment can be provided for cell growth, adherency and proliferation, which also is used as tissue cultures bracket.
Description
Technical field
The present invention relates to fibroin albumen aeroge technical fields, and in particular to a kind of high rebound with three-dimensional bubble structure
The preparation method of sex pilus fibroin aeroge and the high resilience fibroin albumen aeroge being prepared using the preparation method.
Background technique
Aerogel material is a kind of to replace liquid in gel with gas and have the special gel for stablizing three-dimensional net structure.
The density of aeroge is about 0.003~0.5g/cm3, density is minimum can be to 0.001g/cm3, it is most light solid in the world at present
Material has the title of " solid smog ".Nano-void structure inside aeroge assigns its lower thermal conductivity, low-resistivity, low refraction
The advantages that coefficient, makes aeroge have broad application prospects in terms of adsorbent material, heat-insulating heat-preserving material, high performance capacitors.
Aeroge is usually divided into inorganic aerogels, organic polymer aeroge and cellulose aerogels according to raw material sources
Three classes.Inorganic aerogels and organic polymer aeroge are constantly subjected to pay close attention to, and preparation method is constantly updated, also not using raw material
Disconnected optimization, is widely used.But above-mentioned inorganic aerogels and organic polymer aeroge are difficult to biodegrade, biofacies
Capacitive is poor, limits its application popularization.On the other hand, since cellulose aerogels are with resourceful and nontoxic reproducible
Advantage studied temperature persistently overheating in recent years.
The preparation method of cellulose aerogels is mainly divided to following two steps to carry out at present: being the preparation of hydrogel first, so
It cements out the water in gel to obtain aeroge by the methods of supercritical drying or freeze-drying afterwards.But this method obtains
Aeroge the problems such as that there are intensity is not high, easy collapsing, therefore need to study a kind of aerogel material of high intensity.
Fibroin albumen is the natural polymer fibrin extracted from silk, and content accounts for about silk 70%~80%, contains
There are 18 kinds of amino acid.Fibroin itself has good mechanical performance and physicochemical property, as good biocompatibility and degradability,
Low immunogenicity, slow release etc., and pass through the available different form of different disposal, as fiber, solution, powder, film and
Gel is widely used in technical field of biological material.
The research of fibroin aeroge is relatively fewer at present, entitled just like Chinese Patent Application No. 201610199399.X
It is disclosed in the patent of invention of " a kind of composite natral high-molecular gel class material " using using cyclodextrin/reactive polymer
Super molecule inclusion compound be chemically crosslinked gel-forming part as crosslinking agent, and obtain high-intensitive gas through supercritical drying
Gel, but the aeroge manufacturing process is cumbersome, and the crosslinking agent introduced is harmful to human body and environment.For another example Chinese patent application
Numbers 201610526187.8, the gas being prepared in the patent of invention of entitled " a kind of preparation method of fibroin albumen aeroge "
Gel is easily rupturable when compressing, resilience is poor, greatly limits the application range of fibroin albumen aeroge.
Summary of the invention
In view of this, in order to overcome the drawbacks of the prior art, the object of the present invention is to provide a kind of high resilience fibroin eggs
The preparation method of white aeroge, and the high resilience fibroin albumen aeroge being prepared using the preparation method.
In order to achieve the above objectives, the following technical solution is employed by the present invention:
A kind of preparation method of high resilience fibroin albumen aeroge, using Cocoon shell as raw material, comprising the following steps: to
Amides small-molecule substance is added in silk fibroin water solution, is mixed evenly to prepare mixed solution；The mixed solution is poured into appearance
After device, high speed shear is carried out to the mixed solution with blender, the mixed solution is changed into foam-like, by the foam-like
Mixed solution pour into mold and be freeze-dried, obtain fibroin albumen aeroge.
In some embodiments, specifically includes the following steps:
(1) silk cocoon is put into sodium bicarbonate/sodium carbonate buffer of the 0.01M pH=9.5 of slightly boiled (100 DEG C) and is protected
It after holding 30min, takes out silk and is cleaned up with deionized water, repeat the above test three times, to slough the silk gum in cocoon shell, and
Pure silk fibroin protein solution is obtained after lithium bromide dissolution, dialysis, the pure silk fibroin protein solution is diluted to institute with deionized water
State silk fibroin water solution；The concentration of the silk fibroin water solution is 30mg/mL~60mg/mL；
(2) aqueous solution of the amides small-molecule substance of 100mg/mL is configured, is added in Xiang Suoshu silk fibroin water solution
Mixed solution is made in the aqueous solution of amides small-molecule substance, and the concentration of the amides small-molecule substance in the mixed solution is
4mg/mL~15mg/mL, the concentration of fibroin is 30mg/mL~65mg/mL in the mixed solution；
(3) mixed solution is heated to 20-30 DEG C, then poured into the mixed solution in container, at 20-30 DEG C
At a temperature of with blender high speed shear is carried out to the mixed solution, so that the mixed solution is formed a large amount of gas inside container
Bubble, the mixed solution are changed into foam-like；In some embodiments, whipping temp is preferably 25 DEG C；
(4) mixed solution of foam-like is poured into mold and is freeze-dried, obtain the high resilience fibroin albumen
Aeroge.
Some preferred aspects according to the present invention, the amides small-molecule substance are urea or formamide.
Some preferred aspects according to the present invention, the container are domed vessel or bowl.
Some preferred aspects according to the present invention, the blender include at least a lower dimension and are greater than upper dimension
Taper stirring rod.
Preferably, the taper stirring rod includes being located in the middle rotary bar and being connected to several on the rotary bar
The outer edge of a agitating paddle, several agitating paddles is located on the same virtual circular conical surface.In some embodiments, it stirs
For paddle in the form of similar triangle, multiple triangular shaped agitating paddles form a virtual circular conical surface, the side of agitating paddle
On the side of virtual circular conical surface, the bottom edge of agitating paddle is located on the bottom surface of virtual circular conical surface, and rotary bar is connected to circular conical surface
Vertex on.
Some preferred aspects according to the present invention, the shear velocity of high speed shear described in step (3) be 500r/min~
1500r/min, shear time are 20min~60min.
Some preferred aspects according to the present invention, the condition of freeze-drying described in step (4) are at -40 DEG C~-80 DEG C
It is gradually warmed up and is dried in vacuo after freezing 4~8h in environment.
The present invention also provides a kind of high resilience fibroin albumen gas being prepared using preparation method as described above
Gel, the apparent density of the high resilience fibroin albumen aeroge are 0.02g/cm3~0.04g/cm3, when compression ratio is 50%
The compression set of the high resilience fibroin albumen aeroge is 20%~35%.
The principle of the present invention is: fibroin albumen is capable of forming a large amount of bubble in stirring, but simple bubble is stirring
After completion, due to the effect of surface tension, minute bubbles can spontaneously be gathered into air pocket, make the airsetting obtained after freeze-drying
Glue bubble diameter is excessive, causes material that can not restore in compression process since part bubble structure is destroyed.And amides
Small-molecule substance can generate compatible co-mixing system with fibroin albumen, to increase the surface tension of bubble, in this way in high speed
The minute bubbles that shearing is formed will not be gathered into air pocket easily, to improve the energy storage capacity of aeroge.In addition, urea or formyl
Amine can form stronger hydrogen bond with fibroin albumen, intermolecular force be improved, to improve resilience performance.In stirring early period
Bubble is easily broken, and the bubble raffinate after rupture can be made preferably to be gathered in container bottom using domed vessel or bowl
Portion, and the shape of taper stirring rod is that the small lower part in top is big, and the liquid shear of the not formed bubble in bottom can be allowed more abundant, thus
Obtain high resilience fibroin albumen aeroge.
Due to the technical solution more than using, the present invention has following usefulness compared with prior art:
(1) present invention carries out shear treatment by being formed by mixed solution to fibroin albumen and amide micromolecular, cold
Be lyophilized it is dry after obtained aeroge there is unique three-dimensional bubble structure and low-density, high porosity；
(2) the fibroin albumen aeroge prepared by the present invention will not rupture in compression process, have brilliant energy storage energy
Power；
(3) aeroge based on fibroin albumen preparation prepared by the present invention has good biocompatibility and can drop
The advantages such as solution property, can be used for medical dressing, tissue repair etc., can provide for cell growth, adherency and proliferation good
Biotic environment, the aeroge also are used as tissue cultures bracket；
(4) compared to pure silk element aeroge, compression of the aeroge when compression ratio is 50% prepared by the present invention is permanent
Deformation is only 20%~35%, has good compression recovery performance.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the photo in kind of the high resilience fibroin albumen aeroge prepared in the preferred embodiment of the present invention 2；
Fig. 2 is the structural schematic diagram of high resilience fibroin albumen aeroge in the preferred embodiment of the present invention 2；
Fig. 3 is stirring rod schematic diagram in the preferred embodiment of the present invention 2；
Fig. 4 is bowl schematic diagram in the preferred embodiment of the present invention 2；
In attached drawing, high resilience fibroin albumen aeroge -1, occluded air -2, free air -3, taper stirring rod -4 turn
Dynamic stick -5, agitating paddle -6, container -7.
Specific embodiment
In order that those skilled in the art will better understand the technical solution of the present invention, implement below in conjunction with the present invention
Attached drawing in example, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment
Only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work, all should belong to protection of the present invention
Range.
The preparation of 1 silk fibroin water solution of embodiment
80g Cocoon shell is weighed with precision balance, configuration quality score is 0.01M, sodium bicarbonate/carbonic acid of pH=9.5
Cocoon shell is added to after boiling with electromagnetic oven heating by sodium water solution 4000mL, and it is slightly boiled that adjusting electromagnetic oven power keeps aqueous solution to continue
30min takes out silk and is cleaned up with deionized water.
The above test is repeated three times to slough silk gum, silk is then taken out and is placed in drying in 60 DEG C of baking oven and obtain pure silk element
Azelon.Pure fibroin fiber is dissolved in the lithium-bromide solution that concentration is 9.3mol/L by bath raio 20:150, in perseverance
With 60 ± 2 DEG C of dissolution about 1h in warm magnetic stirring apparatus.It takes out to be fitted into bag filter after cooling and seal, be placed in deionized water and dialyse
Pure silk fibroin protein solution is obtained by filtration with absorbent cotton in 3-4d, and the quality of pure silk fibroin protein solution is calculated by dry weighing
Score is placed in spare in 4 DEG C of refrigerators.
The preparation of 2 high resilience fibroin albumen aeroge of embodiment
(1) adjusting the concentration of the pure silk fibroin protein solution in embodiment 1 with deionized water is 35mg/mL, obtains fibroin egg
White water solution.
(2) aqueous solution of urea for configuring 100mg/mL, takes deployed silk fibroin water solution 200mL, to fibroin albumen
Aqueous solution of urea is added in aqueous solution, mixed solution is made, makes the concentration 4mg/mL of urea in mixed solution.
(3) mixed solution is heated to 25 DEG C, then pours into mixed solution in container 7,25 DEG C at a temperature of with stirring
It mixes device and 40min high speed shear is carried out to mixed solution, speed of agitator 1100r/min forms mixed solution inside container 7
A large amount of bubbles, mixed solution are changed into foam-like.
Container 7 in the present embodiment is domed vessel or bowl, and bowl is as shown in figure 4, can make to rupture
Bubble raffinate afterwards is preferably gathered in 7 bottom of container.
Blender in the present embodiment includes two taper stirring rod 4, and the shape of taper stirring rod 4 is the small lower part in top
Greatly, as shown in figure 3, the taper stirring rod 4 in the present embodiment includes being located in the middle rotary bar 5 and being connected on rotary bar 5
Several triangles agitating paddle 6, the outer edge of the agitating paddle 6 of multiple triangles is located on virtual circular conical surface.Specifically,
For agitating paddle 6 in the form of similar triangle, multiple triangular shaped agitating paddles 6 form a virtual circular conical surface, agitating paddle 6
Side be located on the side of virtual circular conical surface, the bottom edge of agitating paddle 6 is located on the bottom surface of virtual circular conical surface, rotary bar 5 connect
On the vertex of virtual circular conical surface, i.e., the stirring rod in the present embodiment is to be gradually reduced by the upward size of lowermost end.In this way
The taper stirring rod 4 of down big up small shape can allow the liquid shear of the not formed bubble in bottom more abundant, to obtain high rebound
Sex pilus fibroin aeroge.
(4) mixed solution of foam-like is poured into mold and is freeze-dried, fibroin albumen aeroge is obtained, such as Fig. 1-2 institute
Show.With the three-dimensional bubble structure of confining gas 2 and free gas 3, three-dimensional bubble knot in high resilience fibroin albumen aeroge 1
What structure referred to is exactly that many minute bubbles are sealed in the structure formed in solid material.
The condition being freeze-dried in the present embodiment is that vacuum is gradually warmed up and carried out after freezing 6h in -45 DEG C of environment to do
It is dry.
The preparation of 3 high resilience fibroin albumen aeroge of embodiment
(1) adjusting the concentration of the pure silk fibroin protein solution in embodiment 1 with deionized water is 45mg/mL, obtains fibroin egg
White water solution.
(2) the formyl amine aqueous solution for configuring 100mg/mL, takes deployed silk fibroin water solution 200mL, Xiang Sisu egg
Formyl amine aqueous solution is added in white water solution, mixed solution is made, makes the concentration 9mg/mL of formamide in mixed solution.
(3) mixed solution is heated to 26 DEG C, then pours into mixed solution in container 7,25 DEG C at a temperature of with stirring
It mixes device and 45min high speed shear is carried out to mixed solution, speed of agitator 900r/min forms mixed solution inside container 7
A large amount of bubbles, mixed solution are changed into foam-like.
Container 7 and blender in the present embodiment is the same as embodiment 2.
(4) mixed solution of foam-like is poured into mold and is freeze-dried, fibroin albumen aeroge is obtained, such as Fig. 1-2 institute
Show.With the three-dimensional bubble structure of confining gas 2 and free gas 3, three-dimensional bubble knot in high resilience fibroin albumen aeroge 1
What structure referred to is exactly that many minute bubbles are sealed in the structure formed in solid material.
The condition being freeze-dried in the present embodiment is that vacuum is gradually warmed up and carried out after freezing 5h in -60 DEG C of environment to do
It is dry.
The preparation of 4 high resilience fibroin albumen aeroge of embodiment
(1) adjusting the concentration of the pure silk fibroin protein solution in embodiment 1 with deionized water is 60mg/mL, obtains fibroin egg
White water solution.
(2) the formyl amine aqueous solution for configuring 100mg/mL, takes deployed silk fibroin water solution 100mL, to above-mentioned silk
Formyl amine aqueous solution is added in fibroin aqueous solution, mixed solution is made, makes the concentration 12mg/mL of formamide in mixed solution.
(3) mixed solution is heated to 22 DEG C, then pours into mixed solution in container 7,25 DEG C at a temperature of with stirring
It mixes device and 60min high speed shear is carried out to mixed solution, speed of agitator 1500r/min forms mixed solution inside container 7
A large amount of bubbles, mixed solution are changed into foam-like.
Container 7 and blender in the present embodiment is the same as embodiment 2.
(4) mixed solution of foam-like is poured into mold and is freeze-dried, fibroin albumen aeroge is obtained, such as Fig. 1-2 institute
Show.Three-dimensional bubble structure with confining gas 2 and free gas 3 in height rebound fibroin albumen aeroge 1, three-dimensional bubble structure
What is referred to is exactly that many minute bubbles are sealed in the structure formed in solid material.
The condition being freeze-dried in the present embodiment is that vacuum is gradually warmed up and carried out after freezing 6h in -70 DEG C of environment to do
It is dry.
The preparation of 5 high resilience fibroin albumen aeroge of embodiment
(1) adjusting the concentration of the pure silk fibroin protein solution in embodiment 1 with deionized water is 50mg/mL, obtains fibroin egg
White water solution.
(2) aqueous solution of urea for configuring 100mg/mL, takes deployed silk fibroin water solution 100mL, to fibroin albumen
Aqueous solution of urea is added in aqueous solution, mixed solution is made, makes the concentration 15mg/mL of urea in mixed solution.
(3) mixed solution is heated to 28 DEG C, then pours into mixed solution in container 7,25 DEG C at a temperature of with stirring
It mixes device and 50min high speed shear is carried out to mixed solution, speed of agitator 500r/min forms mixed solution inside container 7
A large amount of bubbles, mixed solution are changed into foam-like.
Container 7 and blender in the present embodiment is the same as embodiment 2.
(4) mixed solution of foam-like is poured into mold and is freeze-dried, fibroin albumen aeroge is obtained, such as Fig. 1-2 institute
Show.With the three-dimensional bubble structure of confining gas 2 and free gas 3, three-dimensional bubble knot in high resilience fibroin albumen aeroge 1
What structure referred to is exactly that many minute bubbles are sealed in the structure formed in solid material.
The condition being freeze-dried in the present embodiment is that vacuum is gradually warmed up and carried out after freezing 8h in -50 DEG C of environment to do
It is dry.
1 pure silk fibroin aeroge of comparative example
This comparative example does not add amide substance when preparing pure silk fibroin aeroge.Specific preparation process can be found in
Chinese Patent Application No. 201610526187.8, in the patent of invention of entitled " a kind of preparation method of fibroin albumen aeroge "
Preparation process.
The test of embodiment 6 and analysis
Fibroin albumen aeroge test apparent density, compressive strength and the pressure that embodiment 2-5 and comparative example 1 are prepared
Compression permanent deformation the results are shown in Table 1.
1 test result of table
The pure silk element aeroge of embodiment 2-5 is prepared as can be seen from Table 1 fibroin albumen aeroge and comparative example 1
It compares, the modified aeroge of amide substance is added in embodiment 2-5 and is keeping compared with low-density and good compressive strength
Under the premise of, resistance to compression is significantly improved.
The fibroin albumen aeroge being prepared in the present invention has three-dimensional bubble structure, and inside is empty containing a large amount of closings
Gas is not in communication with the outside, and the compression set when compression ratio is 50% is only 20%~35%.In addition, the aeroge is also
Have the characteristics that low-density, high porosity, good biocompatibility and degradability, can be used for medical dressing, tissue is repaired
It is multiple etc., good biotic environment can be provided for cell growth, adherency and proliferation, which also is used as tissue cultures
Bracket.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention, it is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (9)
1. a kind of preparation method of high resilience fibroin albumen aeroge, using Cocoon shell as raw material, which is characterized in that including with
Lower step:
(1) silk gum in Cocoon shell is sloughed with sodium bicarbonate/sodium carbonate buffer of 0.01M pH=9.5, and through bromination
Silk fibroin water solution is obtained after lithium dissolution, dialysis；
(2) aqueous solution of amides small-molecule substance is configured, amide micromolecular object is added in Xiang Suoshu silk fibroin water solution
Mixed solution is made in the aqueous solution of matter, and the concentration of the amides small-molecule substance in the mixed solution is 4mg/mL ~ 15mg/
ML, the concentration of fibroin albumen is 30mg/mL ~ 65mg/mL in the mixed solution；
(3) mixed solution is heated to 20-30 DEG C, then pours into the mixed solution in container, with blender to institute
It states mixed solution and carries out high speed shear, the mixed solution is made to form a large amount of bubbles, the mixed solution transformation inside container
For foam-like；
(4) mixed solution of foam-like is poured into mold and is freeze-dried, obtain the high resilience fibroin albumen airsetting
Glue.
2. the preparation method of high resilience fibroin albumen aeroge according to claim 1, which is characterized in that the amide
Micromolecular substance is urea or formamide.
3. the preparation method of high resilience fibroin albumen aeroge according to claim 1, which is characterized in that the container
For domed vessel or bowl.
4. the preparation method of high resilience fibroin albumen aeroge according to claim 1, which is characterized in that the stirring
Device includes at least the taper stirring rod that a lower dimension is greater than upper dimension.
5. the preparation method of high resilience fibroin albumen aeroge according to claim 4, which is characterized in that the taper
Stirring rod includes the agitating paddle for being located in the middle rotary bar and being connected on the rotary bar, the outside of multiple agitating paddles
Edge is located on the same virtual circular conical surface.
6. the preparation method of high resilience fibroin albumen aeroge according to claim 1, which is characterized in that step (3)
Described in high speed shear shear velocity be 500r/min ~ 1500r/min, shear time be 20min ~ 60min.
7. the preparation method of high resilience fibroin albumen aeroge according to claim 1, which is characterized in that step (4)
Described in the condition of freeze-drying be to heat up and be dried in vacuo after freezing 4 ~ 8h in -40 DEG C ~ -80 DEG C environment.
8. a kind of high resilience fibroin albumen gas that the preparation method using as described in claim 1-7 any one is prepared
Gel.
9. high resilience fibroin albumen aeroge according to claim 8, which is characterized in that the high resilience fibroin egg
The apparent density of white aeroge is 0.02g/cm3 ~ 0.04g/cm3, compression ratio high resilience fibroin albumen airsetting when being 50%
The compression set of glue is 20% ~ 35%.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583836A (en) * | 2004-05-27 | 2005-02-23 | 复旦大学 | Preparation of porous sericin sponge material |
| CN103861149A (en) * | 2014-03-14 | 2014-06-18 | 苏州大学 | Durably-transparent silk fibroin film and preparation method thereof |
| CN104672316A (en) * | 2015-02-11 | 2015-06-03 | 苏州丝美特生物技术有限公司 | Preparation and identification method of domestic silkworm silk fibroin solution |
| CN106009709A (en) * | 2016-06-25 | 2016-10-12 | 南通纺织丝绸产业技术研究院 | Silk fibroin hydrogel adopting IPN (interpenetrating polymer network) structure and preparation method of silk fibroin hydrogel |
| CN106178131A (en) * | 2016-07-04 | 2016-12-07 | 南通纺织丝绸产业技术研究院 | A kind of preparation method of fibroin albumen aeroge |
| CN106700566A (en) * | 2016-12-30 | 2017-05-24 | 南通纺织丝绸产业技术研究院 | Soft and transparent silk fibroin membrane and preparation method thereof |
-
2019
- 2019-01-31 CN CN201910100161.0A patent/CN109851841A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583836A (en) * | 2004-05-27 | 2005-02-23 | 复旦大学 | Preparation of porous sericin sponge material |
| CN103861149A (en) * | 2014-03-14 | 2014-06-18 | 苏州大学 | Durably-transparent silk fibroin film and preparation method thereof |
| CN104672316A (en) * | 2015-02-11 | 2015-06-03 | 苏州丝美特生物技术有限公司 | Preparation and identification method of domestic silkworm silk fibroin solution |
| CN106009709A (en) * | 2016-06-25 | 2016-10-12 | 南通纺织丝绸产业技术研究院 | Silk fibroin hydrogel adopting IPN (interpenetrating polymer network) structure and preparation method of silk fibroin hydrogel |
| CN106178131A (en) * | 2016-07-04 | 2016-12-07 | 南通纺织丝绸产业技术研究院 | A kind of preparation method of fibroin albumen aeroge |
| CN106700566A (en) * | 2016-12-30 | 2017-05-24 | 南通纺织丝绸产业技术研究院 | Soft and transparent silk fibroin membrane and preparation method thereof |
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Application publication date: 20190607 |