CN109841883A - Use method of single-chamber air cathode MFC - Google Patents
Use method of single-chamber air cathode MFC Download PDFInfo
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- CN109841883A CN109841883A CN201910137586.9A CN201910137586A CN109841883A CN 109841883 A CN109841883 A CN 109841883A CN 201910137586 A CN201910137586 A CN 201910137586A CN 109841883 A CN109841883 A CN 109841883A
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- 238000000034 method Methods 0.000 title claims abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 32
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 238000009792 diffusion process Methods 0.000 claims abstract description 11
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 11
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 11
- 238000012544 monitoring process Methods 0.000 claims abstract description 8
- 235000008429 bread Nutrition 0.000 claims abstract description 7
- 238000004891 communication Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 19
- 239000002351 wastewater Substances 0.000 claims description 18
- 235000013405 beer Nutrition 0.000 claims description 14
- 238000005259 measurement Methods 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 6
- 238000005868 electrolysis reaction Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 4
- 235000018417 cysteine Nutrition 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000004064 recycling Methods 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 241001148470 aerobic bacillus Species 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 230000010354 integration Effects 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 claims description 3
- 238000012800 visualization Methods 0.000 claims description 3
- 230000005611 electricity Effects 0.000 abstract description 9
- 239000000446 fuel Substances 0.000 abstract description 9
- 238000011160 research Methods 0.000 abstract description 7
- -1 polytetrafluoroethylene Polymers 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 238000005273 aeration Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 230000009977 dual effect Effects 0.000 description 4
- 230000002906 microbiologic effect Effects 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000003912 environmental pollution Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241001135750 Geobacter Species 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011900 installation process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention provides a single-chamber air cathode MFC, which comprises a reactor, carbon paper with a PTFE (polytetrafluoroethylene) diffusion layer and a voltage detection system, wherein the carbon paper is used for carrying out reaction on a carbon paper; the reactor is divided into an anode chamber and a cathode chamber by carbon paper of a PTFE diffusion layer; the anode chamber takes a carbon brush as a positive electrode, and the cathode chamber takes air as a negative electrode; the Maker's duino core plate and the ADC chip are arranged on the bread board; the ADC chip is in communication connection with the Maker's duino core plate, the anode of the MFC device is connected with the anode of the ADC chip, and the cathode of the MFC device is connected with the cathode of the Maker's duino core plate. The invention also provides a using method of the single-chamber air cathode MFC, which improves the electricity generating efficiency; no pollution and zero emission can be realized; meanwhile, energy consumption aeration is not needed, and the construction and operation cost is lower; optimizing a voltage detection system developed based on an Ardiuno detection platform to realize cloud voltage monitoring; the research cost of the microbial fuel cell is reduced, a new detection means is provided, and the experimenter can conveniently monitor the voltage and analyze the data in real time.
Description
Technical field
The present invention relates to the single chamber field air cathode MFC more particularly to a kind of application methods of single chamber air cathode MFC.
Background technique
Microbiological fuel cell (Microbial fule cells, MFC) is incited somebody to action using microorganism as anode catalyst
Chemical energy in organic matter is converted into the device of electric energy, is a kind of efficient mode of biomass energy utilization and ideal new
Type clean energy resource has many advantages, such as pollution-free, applied widely.Currently, MFC is chiefly used in field of waste water treatment, it is organic handling
Electric energy is obtained while waste water, is the effective way alleviated current energy crisis and solve problem of environmental pollution.In recent years, China
Brewing industry development is swift and violent, and yield ranks first in the world for years, while the generated wastewater discharge in its production
Also it rises with it.Based on organic non-toxic components in beer waste water containing higher concentration, its organic matter is carried out using electricity production bacterium
Chemical energy is converted electric energy by oxygenolysis, carries out the recycling treatment of beer waste water.
In view of the good environmental benefit of MFC and application prospect, there are a large number of researchers carrying out correlative study both at home and abroad.?
In the research application of MFC, requires to carry out real-time voltage detecting to MFC, system is tracked and is analyzed to facilitate, but mesh
Preceding such detector involves great expense, and limits the individual research person of many one-house shows to a certain extent.
To solve the above problems, proposing the application method of single chamber air cathode MFC a kind of in the application.
Summary of the invention
(1) goal of the invention
To solve technical problem present in background technique, the present invention proposes the application method of single chamber air cathode MFC a kind of, mentions
High efficiency of fuel cell generation；Pollution-free, zero-emission can be achieved；Simultaneously without consume energy aeration, build it is less with operating cost；To being based on
The detection electrical voltage system of Ardiuno detection platform exploitation optimizes, and realizes cloud monitoring voltage；Both microbiological fuel cell is reduced
Research cost, provide new detection means, and facilitate experimenter's real-time monitoring voltage, analyze data.
(2) technical solution
To solve the above problems, the invention proposes a kind of single chamber air cathode MFC, the carbon including reactor, PTFE diffusion layer
Paper and voltage detecting system；Reactor is divided into anode chamber and cathode chamber by the carbon paper of PTFE diffusion layer；Made with carbon brush anode chamber
For anode, cathode chamber is using air as cathode；Detection system includes Maker ' s duino core plate, ADC chip and bread
Plate；Maker ' s duino core plate and ADC chip are mounted on bread board；ADC chip and Maker ' s duino core
Plate communication connection, MFC device anode are connect with ADC chip anode, and cathode and Maker ' s duino core plate cathode connect
It connects.
The invention also provides the application methods of single chamber air cathode MFC a kind of, which comprises the following steps:
S1, beer waste water and anaerobic bacteria, aerobic bacteria bacterium solution are subjected to mixed fermentation with the ratio of 1:1, take out acclimation and screening and obtains
Bacterium solution, and mixed in the ratio of 1:5 with diluted beer waste water；
S2, debugging ESP8266 send data module, by being connected to specified WiFi, and are sent and are tested by TCP/IP
Whether data judge to send and succeed to server by return value；
S3, debugging ADC survey voltage module, and the more universal 1.5V dry cell of utilization carrys out the voltage of calibration measurement；
After S4, measurement voltage are errorless, setting is sent to the information of server, requests to send using GET, content be machine ID and
The voltage at the moment, the instruction database for recycling WiFi module packaged, integration measurement voltage and the code for sending data.Setting
The data break sent each time is 15min；
S5, data are checked, on webpage, can be with real time inspection device the case where, the data of transmission, and the visualization of data is provided
And downloading；
S6, voltage detector and fixed value resistance are installed by conducting wire, and connect route；
S7, the mixed liquor for obtaining S1 are added in anode electrolysis pond；
S8, starting battery, the monitoring data after voltage stabilization；And voltage detection system in the process of running is every a timing
Between record primary, service condition is 20 ± 2 DEG C of room temperature.
Preferably, in S1, diluted beer waste water is to be diluted beer waste water and water according to volume ratio 1:1.
Preferably, in S6, in installation process, active carbon bedding electrolytic cell bottom can be used, connects bottom active carbon with carbon brush.
Preferably, in S7, buffer solution is added into anode electrolytic cell, and the phosphate that buffer solution is 0.1mol/L is molten
Liquid.
Preferably, in S7, cysteine solution is added into anode electrolytic cell.
Above-mentioned technical proposal of the invention has following beneficial technical effect: (1) building single chamber air cathode MFC dress
It sets.Device uses air as cathode and eliminates cathode chamber, using oxygen as final electron acceptor, and anode and cathode distance
It is relatively close, it takes up little area, structure is simple, and cathode mass-transfer rate is improved.Expensive with the carbon paper substitution with PTFE diffusion layer
Proton exchange membrane can reduce internal resistance, further increase the power output of MFC, save research cost.Cathode is aerated with dual chamber MFC
It compares, without energy consumption, cathode performance and electricity production power are significantly increased.
(2) product cleans, it is not necessary that chemical reagent is added.Since chemical reagent environmental pollution is larger, single chamber air cathode
MFC cathode is directly contacted in air, it is not necessary that chemical reagent is added, is substituted the potassium ferricyanide of dual chamber MFC cathode, is directly reduced into
This.And product is water, cleanliness without any pollution.
(3) in terms of electricity generation performance, this research is by being added the organic solvents such as cysteine and by solution full of entire
Device builds anaerobic environment, solves the problems, such as that single chamber anode construction dissolved oxygen content is high.And it is micro- by the way that buffer adjusting is added
The pH of biological growth solution improves the modes such as the electric conductivity of solution, optimizes the electricity production condition of microorganism, improves electricity production rate.Except this
Except, since the condition of anode chamber also has large effect to the electricity production rate of air cathode MFC, the carbon brush of former experiment anode is changed
The mode for being paved with the active carbon particle of electrolysis bottom of pond portion for carbon brush connection is conductive, increases the contact area of microorganism and its, with this
Improve the efficiency of fuel cell generation of MFC.
(4) on detection voltage output device, improving original Ardiuno detection system can be by the voltage of generation
Data realize cloud monitoring by being connected to specified WiFi and being uploaded in server.
Specific embodiment
In order to make the objectives, technical solutions and advantages of the present invention clearer, With reference to embodiment to this
Invention is further described.It should be understood that these descriptions are merely illustrative, and it is not intended to limit the scope of the invention.This
Outside, in the following description, descriptions of well-known structures and technologies are omitted, so as not to unnecessarily obscure the concept of the present invention.
Embodiment 1
A kind of single chamber air cathode MFC proposed by the present invention, including reactor, the carbon paper of PTFE diffusion layer and voltage detecting system
System；Reactor is divided into anode chamber and cathode chamber by the carbon paper of PTFE diffusion layer；Anode chamber using carbon brush as anode, cathode chamber with
Air is as cathode；Detection system includes Maker ' s duino core plate, ADC chip and bread board；Maker's duino
Core plate and ADC chip are mounted on bread board；ADC chip and Maker ' s duino core plate communication connection, MFC dress
It sets anode to connect with ADC chip anode, cathode and Maker ' s duino core plate cathode connect.
Embodiment 2
There is above-described embodiment, the invention also provides the application methods of single chamber air cathode MFC a kind of, which is characterized in that including
Following steps:
S1, beer waste water and anaerobic bacteria, aerobic bacteria bacterium solution are subjected to mixed fermentation with the ratio of 1:1, take out acclimation and screening and obtains
Bacterium solution, and mixed in the ratio of 1:5 with diluted beer waste water；
S2, debugging ESP8266 send data module, by being connected to specified WiFi, and are sent and are tested by TCP/IP
Whether data judge to send and succeed to server by return value；
S3, debugging ADC survey voltage module, and the more universal 1.5V dry cell of utilization carrys out the voltage of calibration measurement；
After S4, measurement voltage are errorless, setting is sent to the information of server, requests to send using GET, content be machine ID and
The voltage at the moment, the instruction database for recycling WiFi module packaged, integration measurement voltage and the code for sending data.Setting
The data break sent each time is 15min；
S5, data are checked, on webpage, can be with real time inspection device the case where, the data of transmission, and the visualization of data is provided
And downloading；
S6, voltage detector and fixed value resistance are installed by conducting wire, and connect route；
S7, the mixed liquor for obtaining S1 are added in anode electrolysis pond；
S8, starting battery, the monitoring data after voltage stabilization；And voltage detection system in the process of running is every a timing
Between record primary, service condition is 20 ± 2 DEG C of room temperature.
The working principle of the invention is that microorganism generates during growth metabolism along with electron exchange, native Bacillus
The electronics of generation can be exported release electronics by circuit to extracellular by Geobacter and its similar microbial species in the process
It can form electric current, and soil Bacillus Geobacter and its similar microbial species are generally existing productions under the conditions of different substrates
Electric microorganism；Microbiological fuel cell is a kind of using this quasi-microorganism as catalyst, and organic-fuel oxygenolysis is generated
Chemical energy is changed into the chemical devices of electric energy；MFC is generally made of anode, cathode and diaphragm；Microorganism has in the metabolic process
Electron exchange generates, and exports the electronics of generation to form electric current by circuit, and microorganism is decomposed in anode chamber's catalyzing organic, hair
Raw oxidation reaction generates electronics and proton, and electronics is exported by external circuit, forms electric current, reaches cathode, and it is anti-that reduction occurs with O2
H2O should be generated；The proton that anode generates reaches cathode by the carbon paper with PTEF diffusion layer, participates in the reduction of O2；Utilize this
Processing waste water and generation energy can be combined, generate while murder by poisoning is mitigated or eliminated and be easy to benefit by technical treatment waste water
Electric energy；
Ardiuno detection system can be received the small voltage that microbiological fuel cell generates and be passed through using voltage sensor
The voltage data of collection is uploaded in server by Maker ' s duino core plate by connecting specified WiFi；
And the present invention has following advantages: (1) building single chamber air cathode MFC device；Device uses air as cathode province
But cathode chamber, using oxygen as final electron acceptor, and anode and cathode is closer, and is taken up little area, and structure is simple, cathode
Mass transfer rate is improved；With the expensive proton exchange membrane of the carbon paper substitution with PTFE diffusion layer, internal resistance can be reduced, into one
Step improves the power output of MFC, saves research cost；Compared with dual chamber MFC is aerated cathode, without energy consumption, cathode performance and production
Electrical power is significantly increased；
(2) product cleans, it is not necessary that chemical reagent is added；Since chemical reagent environmental pollution is larger, single chamber air cathode MFC yin
It is extremely directly contacted in air, it is not necessary that chemical reagent is added, substitutes the potassium ferricyanide of dual chamber MFC cathode, directly reduce cost；And
And product is water, cleanliness without any pollution；
(3) on detection voltage output device, improving original Ardiuno detection system can be by the voltage data of generation
By being connected to specified WiFi and being uploaded in server, cloud monitoring is realized.
In an alternative embodiment, in S1, diluted beer waste water is by beer waste water and water according to volume ratio 1:1
It is diluted.
In an alternative embodiment, in S6, in installation process, active carbon bedding electrolytic cell bottom can be used, carbon brush is used
Bottom active carbon is connected, the mode that carbon brush is changed to the active carbon particle that carbon brush connection is paved with electrolysis bottom of pond portion is conductive, increases micro-
The contact area of biology and its, so as to improve the efficiency of fuel cell generation of MFC.
In an alternative embodiment, in S7, buffer solution is added into anode electrolytic cell, and buffer solution is
The phosphate solution of 0.1mol/L adjusts the pH of microorganism growth solution by the way that buffer is added, improves the electric conductivity etc. of solution
Mode optimizes the electricity production condition of microorganism, improves electricity production rate.
In an alternative embodiment, in S7, cysteine solution is added into anode electrolytic cell, by the way that half Guang is added
The organic solvents such as propylhomoserin and solution is built into anaerobic environment full of whole device, solves single chamber anode construction dissolved oxygen content
High problem.
It should be understood that above-mentioned specific embodiment of the invention is used only for exemplary illustration or explains of the invention
Principle, but not to limit the present invention.Therefore, that is done without departing from the spirit and scope of the present invention is any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.In addition, appended claims purport of the present invention
Covering the whole variations fallen into attached claim scope and boundary or this range and the equivalent form on boundary and is repairing
Change example.
Claims (6)
1. a kind of single chamber air cathode MFC, which is characterized in that including reactor, the carbon paper of PTFE diffusion layer and voltage detecting system
System；Reactor is divided into anode chamber and cathode chamber by the carbon paper of PTFE diffusion layer；Anode chamber using carbon brush as anode, cathode chamber with
Air is as cathode；Detection system includes Maker ' s duino core plate, ADC chip and bread board；Maker's duino
Core plate and ADC chip are mounted on bread board；ADC chip and Maker ' s duino core plate communication connection, MFC dress
It sets anode to connect with ADC chip anode, cathode and Maker ' s duino core plate cathode connect.
2. single chamber air cathode MFC according to claim 1, it is also proposed that a kind of user of single chamber air cathode MFC
Method, which comprises the following steps:
S1, beer waste water and anaerobic bacteria, aerobic bacteria bacterium solution are subjected to mixed fermentation with the ratio of 1:1, take out acclimation and screening and obtains
Bacterium solution, and mixed in the ratio of 1:5 with diluted beer waste water；
S2, debugging ESP8266 send data module, by being connected to specified WiFi, and are sent and are tested by TCP/IP
Whether data judge to send and succeed to server by return value；
S3, debugging ADC survey voltage module, and the more universal 1.5V dry cell of utilization carrys out the voltage of calibration measurement；
After S4, measurement voltage are errorless, setting is sent to the information of server, requests to send using GET, content be machine ID and
The voltage at the moment, the instruction database for recycling WiFi module packaged, integration measurement voltage and the code for sending data.Setting
The data break sent each time is 15min；
S5, data are checked, on webpage, can be with real time inspection device the case where, the data of transmission, and the visualization of data is provided
And downloading；
S6, voltage detector and fixed value resistance are installed by conducting wire, and connect route；
S7, the mixed liquor for obtaining S1 are added in anode electrolysis pond；
S8, starting battery, the monitoring data after voltage stabilization；And voltage detection system in the process of running is every a timing
Between record primary, service condition is 20 ± 2 DEG C of room temperature.
3. the application method of single chamber air cathode MFC according to claim 2 a kind of, which is characterized in that diluted in S1
Beer waste water is to be diluted beer waste water and water according to volume ratio 1:1.
4. the application method of single chamber air cathode MFC according to claim 2 a kind of, which is characterized in that in S6, installed
Cheng Zhong, can use active carbon bedding electrolytic cell bottom, connect bottom active carbon with carbon brush.
5. the application method of single chamber air cathode MFC according to claim 2 a kind of, which is characterized in that in S7, to anode
Buffer solution is added in electrolytic cell, and buffer solution is the phosphate solution of 0.1mol/L.
6. the application method of single chamber air cathode MFC according to claim 2 a kind of, which is characterized in that in S7, to anode
Cysteine solution is added in electrolytic cell.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112432987A (en) * | 2020-11-06 | 2021-03-02 | 南开大学 | Method for constructing toxicity early warning system of photoautotrophic oxygen reduction biological cathode sensor |
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