CN109828075B - Method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves - Google Patents
Method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves Download PDFInfo
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- CN109828075B CN109828075B CN201910230489.4A CN201910230489A CN109828075B CN 109828075 B CN109828075 B CN 109828075B CN 201910230489 A CN201910230489 A CN 201910230489A CN 109828075 B CN109828075 B CN 109828075B
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Abstract
The invention relates to a method for identifying indian horseradish lignin and phoenix tail horseradish lignin in moringa leaves, which is characterized in that an aqueous extract of the moringa leaves is purified by LSA5B type macroporous adsorption resin and then is identified by thin-layer chromatography at 0-10 ℃, so that a special identification method for the indian horseradish lignin and the phoenix tail horseradish lignin in the moringa leaves is provided, and the quality and the curative effect of a medicine are effectively ensured in production and application of a moringa leaf preparation.
Description
Technical Field
The invention relates to a compound identification method, in particular to an identification method of indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves, belonging to the technical field of medicines.
Background
Moringa oleifera (A), (B), (CMoringa oleiferamLam.) native to India and Africa regions, is a plant of the Moringaceae Moringa genus. At present, the plants are widely planted in Asia, African tropical and subtropical regions, and are praised as 'strange trees', 'medical treasure box' and the like because of rapid growth, rich nutrition and high medicinal value.
The moringa leaves contain rich nutrient substances and medicinal components, and researches show that the moringa leaf powder has medicinal effects of resisting bacteria, diminishing inflammation and the like, the effective components of the moringa leaf powder are mainly alkaloids, the content of the alkaloids distributed in the leaves is obviously higher than that of the alkaloids distributed in the stems, and the maximum total alkaloids is 4.62 mg/g. Researches show that the alkaloid in the moringa leaves has broad-spectrum antibacterial effect on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, enterococcus aerogenes and the like, particularly has obvious antibacterial effect on indian moringa oleifera and phoenix tailed moringa oleifera, and can be used for producing antibacterial agents.
The existing literature has more methods for identifying alkaloids, but the identification of the alkaloids in the moringa leaves is less, and the identification of main bactericidal components of the moringa oleifera and the phoenix tail moringa oleifera is not reported. The product quality can not be effectively ensured in production or use, so that the curative effect of the medicine can not be ensured.
Due to the fact that the chemical properties of the indian moringa oleifera and the phoenix tailed moringa oleifera are very similar and difficult to separate and identify, through a series of researches, the fact that the indian moringa oleifera and the phoenix tailed moringa oleifera in the moringa leaves can be identified by a TLC method that the moringa oleifera and the phoenix tailed moringa oleifera are firstly extracted and purified by water and then developed twice at 0-10 ℃ is found.
Disclosure of Invention
The invention aims to provide a method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves. The identification method of the indian moringa oleifera and phoenix tail moringa oleifera in the moringa leaves comprises the following steps: purifying the aqueous extract of Moringa oleifera leaves with LSA5B type macroporous adsorbent resin, and concentrating the chloroform eluate to obtain test solution. Dissolving indian capsaicin and phoenix tail capsaicin as reference substances in chloroform to obtain reference solutions. Spotting the test solution and the control solution on the same silica gel HF254 thin-layer chromatography glass plate by using a capillary tube, presaturating with ammonia vapor for a certain time at room temperature, developing twice at 0-10 ℃ by using chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent, drying at 105 ℃, spraying a special color developing agent for the improved bismuth potassium iodide alkaloid, and inspecting under sunlight.
Preferably, the sample solution is eluted with LSA5B macroporous resin to remove a large amount of water-soluble impurities such as pigment and polysaccharide.
Preferably, the ammonia vapor is pre-saturated for 15-25 min.
Preferably, the developing temperature of the developing solution is 0-10 ℃ by taking chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent.
Preferably, the developing times are twice by using chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent.
Preferably, chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) is used as a developing agent, the thin-layer plate is developed to 1/3-2/3 of the front edge of the thin-layer plate for the first time, and is developed to 3/4-1 of the front line of the thin-layer plate for the second time.
Drawings
FIG. 1: example 1 thin layer identification scheme: 1. three batches of horseradish tree leaf test solutions (batch numbers of 7L1103, 7N0122 and 8L1005 respectively) are provided at 2 and 4, and reference substance solution is provided at 3.
FIG. 2: example 2 thin layer identification profile: 1. three batches of horseradish tree leaf test solutions (batch numbers of 7L1103, 7N0122 and 8L1005 respectively) are provided at 2 and 4, and reference substance solution is provided at 3.
FIG. 3: example 3 thin layer identification profile: 1. three batches of horseradish tree leaf test solutions (batch numbers 7L1103, 7N0122 and 8L1005 respectively) are provided at 3 and 4, and reference substance solution is provided at 2.
Detailed Description
Example 1 an aqueous extract of moringa leaves was purified by using LSA5B type macroporous adsorbent resin, which was first purified by using water to remove a large amount of pigments, saccharides and water-soluble proteins, and then eluted with chloroform, and the chloroform eluate was concentrated to give a test solution. Dissolving indian capsaicin reference substance and phoenix tail capsaicin reference substance in chloroform respectively to obtain reference substance solutions. Dropping the test solution and the reference solution on the same silica gel HF254 thin-layer chromatography glass plate by using a capillary tube, presaturating the glass plate with ammonia vapor at room temperature for 20min, then spreading the glass plate twice at 0-10 ℃ by using chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent, spreading the glass plate twice to 1/2 at the front edge of the thin-layer plate for the first time, spreading the glass plate twice to the front line of the thin-layer plate, taking the glass plate out, drying the glass plate at 105 ℃, spraying a special color developing agent for the improved bismuth potassium iodide alkaloid, and inspecting the glass plate under sunlight.
Example 2 an aqueous extract of moringa leaves was purified by using LSA5B type macroporous adsorbent resin, which was first purified by using water to remove a large amount of pigments, saccharides and water-soluble proteins, and then eluted with chloroform, and the chloroform eluate was concentrated to give a test solution. Dissolving indian capsaicin reference substance and phoenix tail capsaicin reference substance in chloroform respectively to obtain reference substance solutions. Dropping the test solution and the reference solution on the same silica gel HF254 thin-layer chromatography glass plate by using a capillary tube, presaturating the glass plate with ammonia vapor at room temperature for 25min, then spreading the glass plate twice at 0-10 ℃ by using chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent, spreading the glass plate twice to 1/3 at the front edge of the thin-layer plate for the first time, spreading the glass plate twice to 4/5 along the front line of the thin-layer plate for the second time, taking out the glass plate, drying the glass plate at 105 ℃, spraying a special color developing agent for the improved potassium bismuth iodide alkaloid, and inspecting the glass plate under sunlight.
Example 3 an aqueous extract of moringa leaves was purified by using LSA5B type macroporous adsorbent resin, which was first purified by using water to remove a large amount of pigments, saccharides and water-soluble proteins, and then eluted with chloroform, and the chloroform eluate was concentrated to give a test solution. Dissolving indian capsaicin reference substance and phoenix tail capsaicin reference substance in chloroform respectively to obtain reference substance solutions. Dropping the test solution and the reference solution on the same silica gel HF254 thin-layer chromatography glass plate by using a capillary tube, presaturating the glass plate with ammonia vapor at room temperature for 15min, then spreading the glass plate twice at 0-10 ℃ by using chloroform-ethyl acetate-glacial acetic acid-water (3.5: 2: 0.03: 0.5) as a developing agent, spreading the glass plate twice to 2/3 at the front edge of the thin-layer plate for the first time, spreading the glass plate twice to the front line of the thin-layer plate, taking the glass plate out, drying the glass plate at 105 ℃, spraying a special color developing agent for the improved bismuth potassium iodide alkaloid, and inspecting the glass plate under sunlight.
Claims (4)
1. A method for identifying indian horseradish lignin and phoenix tail horseradish lignin in horseradish tree leaves comprises the following steps: purifying the aqueous extract of Moringa oleifera leaves with LSA5B type macroporous adsorbent resin, and concentrating chloroform eluate to obtain test solution; respectively dissolving indian capsaicin and phoenix tail capsaicin as reference substances in chloroform to obtain reference solutions; spotting the test solution and the control solution on the same silica gel HF254 thin-layer chromatography glass plate by using a capillary tube, presaturating the test solution and the control solution with ammonia vapor for a certain time at room temperature, developing the pre-saturated solution twice at 0-10 ℃ by using chloroform-ethyl acetate-glacial acetic acid-water as a developing agent in a volume ratio of 3.5:2: 0.03:0.5, drying the developed solution at 105 ℃, spraying a special color developing agent for the improved bismuth potassium iodide alkaloid, and inspecting the developed solution under sunlight.
2. The method for identifying indian horseradish lignin and phoenix tail horseradish lignin in moringa leaves according to claim 1, wherein a test solution is eluted by using LSA5B type macroporous adsorption resin to remove a large amount of pigments and polysaccharides.
3. The method for identifying indian horseradish lignin and phoenix tail horseradish lignin in moringa leaves as claimed in claim 1, wherein the ammonia vapor is pre-saturated for 15-25 min.
4. The method for identifying the indian horseradish lignin and the phoenix tailed horseradish lignin in the horseradish tree leaves as claimed in claim 1, wherein chloroform-ethyl acetate-glacial acetic acid-water with the volume ratio of 3.5:2: 0.03:0.5 is used as a developing agent, the chloroform-ethyl acetate-glacial acetic acid-water is firstly developed to 1/3-2/3 of the front edge of the thin-layer plate, and the chloroform-ethyl acetate-glacial acetic acid-water is secondly developed to 3/4-1 of the front line of the thin-layer plate.
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| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2821700A1 (en) * | 2012-08-01 | 2014-02-01 | General Electric Company | Material and exhaust gas system and method for using the same |
| CN103980291A (en) * | 2014-04-29 | 2014-08-13 | 天津大学 | Method for extracting pterygsspermine from moringa oleifera lam roots |
| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
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