CN109828069A - A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print - Google Patents
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print Download PDFInfo
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Abstract
The invention discloses a kind of measuring methods of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, its chromatographic condition includes: that chromatographic column is octadecylsilane chemically bonded silica chromatographic column, using acetonitrile or methanol as mobile phase A, using aqueous formic acid, acetic acid aqueous solution or phosphate aqueous solution as Mobile phase B, detector is DAD detector, carries out gradient elution.The present invention is quick, easy, comprehensive, accurate and reliable, is suitble to detect the finger-print of yihuang decoction.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines more particularly to a kind of measuring methods of Chinese medicine compound prescription yihuang decoction finger-print.
Background technique
The prescription of " yihuang decoction " "Fu Qingzhu works on obstetric" written by the Qing Dynasty Fu Shan, " married woman has a leukorrhagia and color Huang person, just like
Yellow tea extract, stink smell, so-called yellowish leukorrhea are.... method preferably mends the void of conception vessel, and the inflammation of clear kidney fire, then maybe.Fang Yong
Yihuang decoction ".
" yihuang decoction " composition: one liang of Chinese yam (stir-fry), one liang of Gorgon fruit (stir-fry), two money of Cortex Phellodendri (salt water stir-fry), semen plantaginis one
Money (parched with wine), ten pieces of gingko (broken).
" yihuang decoction " is major function: under kidney deficiency humid tropics.The sticky amount of leukorrhagia is more, color Huang such as strong tea juice, stink smell, tongue
It is red, yellow and greasy fur person.Modern clinic application is mainly used for (1) cervical erosion；(2) chronic pelvic inflammatory disease；(3) treatment of vaginitis etc..
Traditional Chinese medicine fingerprint borrows DNA fingerprinting and develops.What is grown up earliest is chemical composition of Chinese materia medica chromatography
Finger-print, especially high performance liquid chromatography (HPLC) finger-print.HPLC has high resolution ratio, can by different chemistry at
It is separated and forms the different chromatographic peak of height and form a chromatogram, the height and peak area of these chromatographic peaks generation respectively
The various different chemical compositions of table and its content.Entire chromatogram characterize chemical component contained by the sample number and amount it is big
It is small.
Traditional Chinese medicine fingerprint occupies critically important status in modern and following traditional Chinese medicine quality control, meets in Chinese medicine
The characteristics of medicine globality and ambiguity, can analyze the letter that the type and content of effective component and invalid components are distributed in Chinese medicine
Breath, as more and more technologies are constantly applied to the research of traditional Chinese medicine fingerprint, traditional Chinese medicine fingerprint must be in traditional Chinese medicine quality
Many-sided play such as control, herbal medicine efficacy composition Study, Chinese medicine study on mechanism more importantly acts on.
Traditional Chinese medicine fingerprint has more advantage than DNA fingerprinting, not only there is the embodiment (number of various chemical components of feature
The relative position and --- retention time) Qualitive test use, the concept for the amount of being also presented, height and the peak area characterization at peak can be made
The content of some chemical component, and the ratio of the peak height (or peak area) at each peak embodies between various chemical components opposite contains
Amount.Qualitative and quantitative combination assigns traditional Chinese medicine fingerprint bigger effect.Traditional Chinese medicine fingerprint can not only carry out individual, certain
The identification of " uniqueness " of species can also link up with the feature of its " amount " and other systems, such as hang with drug efficacy study result
Hook.The finger-print of ginkgo biloba extract embodies its contained 33 GINKGO BILOBA EXTRACT (chemical component) and respective content.Germany is through 30
The chemical component and drug effect correlative study of many years finds that about 24% GINKGO BILOBA EXTRACT and the ginkgo of about 6% ginkgolides composition extract
It is exactly a typical case that object (having corresponding finger-print to control its ingredient and relative amount), which has best curative effect,.Therefore,
Traditional Chinese medicine fingerprint is not only a kind of traditional Chinese medicine quality control mode and technology, can more develop into a kind of using various fingerprint images
It composes to carry out the research system and research mode of tcm theory (complication system) and new drug development[1].(bibliography: [1]) sieve state
[J] Chinese patent drug, 2000,22 (6): 395.) are composed and its applied to peace, Wang Yiming, Cao Jin, multi-dimensional information characteristics.
In order to control the quality of Chinese medicine compound prescription yihuang decoction, the type and content distribution of each ingredient in Chinese medicine yihuang decoction is analyzed
Information, herbal medicine efficacy ingredient, Chinese medicine mechanism of action etc., needs to establish corresponding fingerprint control method.And in existing skill
In art, be suitble to quickly, easy, accurate analysis method can be used for the numbers, opposite of the various chemical components of Chinese medicine yihuang decoction
The detection of the relevant informations such as the characteristic differentiation of position and content distribution.Therefore, it is necessary to establish high performance liquid chromatography (HPLC) measurement
The finger-print of Chinese medicine yihuang decoction.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of surveys of Chinese medicine compound prescription yihuang decoction finger-print
Determine method, the present invention is quick, easy, comprehensive, accurate and reliable, is suitble to detect the finger-print of yihuang decoction.
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print proposed by the present invention, using high performance liquid chromatography,
Its chromatographic condition includes: that chromatographic column is octadecylsilane chemically bonded silica chromatographic column, using acetonitrile or methanol as mobile phase A, with first
Aqueous acid, acetic acid aqueous solution or phosphate aqueous solution are Mobile phase B, and detector is DAD detector, carry out gradient elution；
The gradient elution program are as follows: in 0-40min, the volume ratio of mobile phase A and Mobile phase B from 5:95 at the uniform velocity gradual change to
30:70；In 40-55min, the volume ratio of mobile phase A and Mobile phase B is from 30:70 at the uniform velocity gradual change to 95:5；In 55-60min, stream
The volume ratio of dynamic phase A and Mobile phase B is from 95:5 at the uniform velocity gradual change to 5:95；In 60-65min, the volume of mobile phase A and Mobile phase B
Than for 5:95.
Preferably, the length of chromatographic column is 250mm, diameter 4.6mm.
Preferably, the octadecylsilane chemically bonded silica partial size of chromatographic column is 2-10 μm.
Preferably, the octadecylsilane chemically bonded silica partial size of chromatographic column can be 2,3,4,5,6,7,8,9 or 10 μm.
Preferably, the octadecylsilane chemically bonded silica partial size of chromatographic column is 5 μm.
Octadecylsilane chemically bonded silica chromatographic column is available on the market, and there are many brands, such as: AgilentHC-
C18 etc..
Preferably, the volume parts of aqueous formic acid, acetic acid aqueous solution or phosphate aqueous solution are 0.01-1.5%.
Preferably, the volume parts of aqueous formic acid, acetic acid aqueous solution or phosphate aqueous solution all can be 0.01,0.05,
0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,1,1.1,1.2,1.3,1.4 or 1.5%.
Preferably, the volume fraction of aqueous formic acid, acetic acid aqueous solution or phosphate aqueous solution is 0.5%.
Preferably, mobile phase A is acetonitrile.
Preferably, Mobile phase B is phosphate aqueous solution.
Preferably, flow velocity 0.5-1.5ml/min.
Preferably, flow velocity can be 0.5,0.6,0.7,0.8,0.9,1,1.1,1.2,1.3,1.4 or 1.5ml/min.
Preferably, flow velocity 1.0ml/min.
Preferably, column temperature is 20-40 DEG C.
Preferably, column temperature can for 20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,
37,38,39 or 40 DEG C.
Preferably, column temperature is 35 DEG C.
Preferably, Detection wavelength 280-290nm.
Preferably, Detection wavelength can be 280,281,282,283,284,285,286,287,288,289 or 290nm.
Preferably, Detection wavelength 286nm.
Preferably, sample volume is 8-12 μ L.
Preferably, sample volume can be 8,9,10,11 or 12 μ L.
Preferably, sample volume is 10 μ L.
Preferably, the specific steps of measurement are as follows: prepare test solution, control medicinal material solution, successively sample introduction, record chromatography
Figure.
Preferably, control medicinal material is Chinese yam, Gorgon fruit, Cortex Phellodendri, semen plantaginis and gingko.
Preferably, test solution preparation method are as follows: yihuang decoction dried cream powder is weighed, solubilizer ultrasound 40-50min shakes up,
It lets cool filtration and obtains test solution.
Preferably, the preparation method of control medicinal material solution are as follows: weigh control medicinal material dried cream powder, solubilizer ultrasound 40-
50min shakes up, and lets cool filtration and obtains control medicinal material solution.
Control medicinal material dried cream powder is identical with the preparation method of yihuang decoction dried cream powder.
Above-mentioned dried cream powder is this field routine term.
Preferably, the mixed liquor for the phosphate aqueous solution that solvent is methanol and volume fraction is 0.1%, methanol and phosphoric acid are water-soluble
The volume ratio of liquid is 2-3:3-2.
Preferably, the volume ratio of methanol and phosphate aqueous solution is 1:1.
The present inventor carries out determining fingerprint pattern, the result is shown in Figure 1 and table 1 to yihuang decoction:
The measurement result of 1 yihuang decoction finger-print of table
| Peak serial number | Retention time min | Separating degree R | Exclusive peak |
| 1 | 6.872 | --- | Gorgon fruit |
| 2 | 7.472 | 1.642 | Chinese yam |
| 3 | 9.420 | 5.354 | Gorgon fruit |
| 4 | 13.635 | 12.475 | --- |
| 5 | 18.081 | 13.668 | Gingko |
| 6 | 19.194 | 3.361 | Gingko |
| 7 | 20.892 | 5.299 | Cortex Phellodendri |
| 8 | 24.892 | 11.504 | Cortex Phellodendri |
| 9 | 32.077 | 20.529 | Semen plantaginis |
| 10 | 37.320 | 14.062 | --- |
| 11 | 39.829 | 6.467 | --- |
| 12 | 42.526 | 5.418 | Cortex Phellodendri |
Yihuang decoction detects 14 chromatographic peaks altogether as can be seen from the above table, wherein 1, No. 3 is the exclusive peak of Gorgon fruit, and No. 2 are mountains
The exclusive peak of medicine, 5, No. 6 are the exclusive peaks of gingko, and 7,8, No. 12 are the exclusive peaks of Cortex Phellodendri, and No. 9 are the exclusive peaks of semen plantaginis.
Compared with prior art, beneficial effects of the present invention are as follows:
The method of the present invention is by comprehensively considering analysis chromatographic column, mobile phase, flow velocity, column temperature, gradient elution program to separation
The combined influence of detection so that testing result has reached optimization, have the advantages that it is quick, easy, comprehensive, accurate and reliable, fit
The finger-print for closing detection yihuang decoction measures Chinese medicine yihuang decoction at 286nm wavelength, and appearance is more, and peak shape is good, can clearly be in
The exclusive characteristic peak for revealing Gorgon fruit, Chinese yam, gingko, semen plantaginis, Cortex Phellodendri, is easy to identify, and the fingerprint pattern technology content of foundation is high,
The information of reflection is more complete, avoids the unicity and one-sidedness of the control of Chinese medicine yihuang decoction quality, can effectively reflect that Chinese medicine is easily yellow
The quality of soup, to can guarantee the stability of quality of medicinal material and the validity of clinical application and safety.
Detailed description of the invention
Fig. 1 is the yihuang decoction finger-print that the embodiment of the present invention 1 measures.
Fig. 2 is the Gorgon fruit control medicinal material finger-print that the embodiment of the present invention 1 measures.
Fig. 3 is the gingko control medicinal material finger-print that the embodiment of the present invention 1 measures.
Fig. 4 is the semen plantaginis control medicinal material finger-print that the embodiment of the present invention 1 measures.
Fig. 5 is the Chinese yam control medicinal material finger-print that the embodiment of the present invention 1 measures.
Fig. 6 is the Cortex Phellodendri control medicinal material finger-print that the embodiment of the present invention 1 measures.
Fig. 7 is the yihuang decoction finger-print that the embodiment of the present invention 2 measures.
Fig. 8 is the yihuang decoction finger-print that the embodiment of the present invention 3 measures.
Fig. 9 is the yihuang decoction finger-print that the embodiment of the present invention 4 measures.
Figure 10 is the yihuang decoction finger-print that the embodiment of the present invention 5 measures.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, chromatographic condition packet
Include: chromatographic column is octadecyl silane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), using acetonitrile as mobile phase A, with volume point
Number is that 0.5% phosphate aqueous solution is Mobile phase B, and detector is DAD detector, Detection wavelength 286nm, flow velocity 1.0ml/
Min, column temperature are 35 DEG C, carry out gradient elution；
The gradient elution program are as follows: in 0-40min, the volume ratio of mobile phase A and Mobile phase B from 5:95 at the uniform velocity gradual change to
30:70；In 40-55min, the volume ratio of mobile phase A and Mobile phase B is from 30:70 at the uniform velocity gradual change to 95:5；In 55-60min, stream
The volume ratio of dynamic phase A and Mobile phase B is from 95:5 at the uniform velocity gradual change to 5:95；In 60-65min, the volume of mobile phase A and Mobile phase B
Than for 5:95.
Solution is prepared:
Chinese yam control medicinal material solution: weighing Chinese yam control medicinal material 1g, and Chinese yam is made according to yihuang decoction dry cream powder preparation technique
Control medicinal material dried cream powder, solubilizer 10mL ultrasound 45min, shakes up, and lets cool filtration and obtains Chinese yam control medicinal material solution.
Gorgon fruit control medicinal material solution: weighing Gorgon fruit control medicinal material 1g, and Gorgon fruit is made according to yihuang decoction dry cream powder preparation technique
Control medicinal material dried cream powder, solubilizer 10mL ultrasound 45min, shakes up, and lets cool filtration and obtains Gorgon fruit control medicinal material solution.
Cortex Phellodendri control medicinal material solution: weighing Cortex Phellodendri control medicinal material 1g, and Cortex Phellodendri is made according to yihuang decoction dry cream powder preparation technique
Control medicinal material dried cream powder, solubilizer 10mL ultrasound 45min, shakes up, and lets cool filtration and obtains Cortex Phellodendri control medicinal material solution.
Semen plantaginis control medicinal material solution: weighing semen plantaginis control medicinal material 1g, is made according to yihuang decoction dry cream powder preparation technique
Semen plantaginis control medicinal material dried cream powder, solubilizer 10mL ultrasound 45min, shakes up, and lets cool filtration and obtains semen plantaginis control medicinal material solution.
Gingko control medicinal material solution: weighing gingko control medicinal material 1g, and gingko is made according to yihuang decoction dry cream powder preparation technique
Control medicinal material dried cream powder, solubilizer 10mL ultrasound 45min, shakes up, and lets cool filtration and obtains gingko control medicinal material solution.
Test solution: yihuang decoction dried cream powder 1.0g, solubilizer 20mL ultrasound 45min is taken to shake up, let cool filtration and supplied
Test sample solution.
The mixed liquor for the phosphate aqueous solution that above-mentioned solvent is methanol and volume fraction is 0.1%, methanol and phosphoric acid are water-soluble
The volume ratio of liquid is 1:1；Above-mentioned ultrasonic power is 500W, 40KHz.
Test operation: taking each reference substance solution and 10 μ L of test solution successively sample introduction respectively, records chromatogram.
Typical map is shown in that Fig. 1-6, Fig. 1 are the yihuang decoction finger-print that the embodiment of the present invention 1 measures；Fig. 2 is that the present invention is real
Apply the Gorgon fruit control medicinal material finger-print that example 1 measures；Fig. 3 is the gingko control medicinal material finger-print that the embodiment of the present invention 1 measures；
Fig. 4 is the semen plantaginis control medicinal material finger-print that the embodiment of the present invention 1 measures；Fig. 5 is the Chinese yam pair that the embodiment of the present invention 1 measures
According to medicinal materials fingerprint；Fig. 6 is the Cortex Phellodendri control medicinal material finger-print that the embodiment of the present invention 1 measures；
Pass through contrasting for the finger-print to the full side of yihuang decoction, single medicinal material, Gorgon fruit, Chinese yam, gingko, semen plantaginis, Cortex Phellodendri
Exclusive peak number and its retention time it is as follows:
The exclusive peak of Gorgon fruit: No. 1 peak (6.872min), No. 3 peaks (9.420min)；
The exclusive peak of Chinese yam: No. 2 peaks (7.472min)；
The exclusive peak of gingko: No. 5 peaks (18.081min), No. 6 peaks (19.194min)；
The exclusive peak of semen plantaginis: No. 9 peaks (32.077min)；
The exclusive peak of Cortex Phellodendri: No. 7 peaks (20.892min), No. 8 peaks (24.892min), No. 12 peaks (42.526min).
It using the detection method in the embodiment of the present invention 1, is found in conjunction with Fig. 1, it is easily yellow that Chinese medicine is measured at 286nm wavelength
Soup, appearance is more, and peak shape is good, can clearly appear from out the exclusive characteristic peak of Gorgon fruit, Chinese yam and gingko, semen plantaginis, Cortex Phellodendri, be easy to
Identify, the fingerprint pattern technology content of foundation is high, and the information of reflection is more complete, avoids the single of Chinese medicine yihuang decoction quality control
Property and one-sidedness, can effectively reflect the quality of Chinese medicine yihuang decoction, to can guarantee the stability and clinical application of quality of medicinal material
Validity and safety.
Embodiment 2
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, chromatographic condition packet
Include: chromatographic column is octadecyl silane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), using acetonitrile as mobile phase A, with volume point
Number is that 0.5% aqueous formic acid is Mobile phase B, and detector is DAD detector, Detection wavelength 286nm, flow velocity 1.0ml/
Min, column temperature are 35 DEG C, carry out gradient elution；The gradient elution process is the same as embodiment 1.
Solution is prepared and test operation is the same as embodiment 1.
Typical map is shown in that Fig. 7, Fig. 7 are the yihuang decoction finger-print that the embodiment of the present invention 2 measures.
Embodiment 3
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, chromatographic condition packet
Include: chromatographic column is octadecyl silane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), using acetonitrile as mobile phase A, with volume point
Number is that 0.5% acetic acid aqueous solution is Mobile phase B, and detector is DAD detector, Detection wavelength 286nm, flow velocity 1.0ml/
Min, column temperature are 35 DEG C, carry out gradient elution；The gradient elution process is the same as embodiment 1.
Solution is prepared and test operation is the same as embodiment 1.
Typical map is shown in that Fig. 8, Fig. 8 are the yihuang decoction finger-print that the embodiment of the present invention 3 measures.
Embodiment 4
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, chromatographic condition packet
Include: chromatographic column is octadecyl silane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), using methanol as mobile phase A, with volume point
Number is that 0.5% phosphate aqueous solution is Mobile phase B, and detector is DAD detector, Detection wavelength 286nm, flow velocity 1.0ml/
Min, column temperature are 35 DEG C, carry out gradient elution；The gradient elution process is the same as embodiment 1.
Solution is prepared and test operation is the same as embodiment 1.
Typical map is shown in that Fig. 9, Fig. 9 are the yihuang decoction finger-print that the embodiment of the present invention 4 measures.
Embodiment 5
A kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, using high performance liquid chromatography, chromatographic condition packet
Include: chromatographic column is Agilent HC-C18 (250 × 4.6mm, 5 μm), using acetonitrile as mobile phase A, with volume fraction for 0.5% phosphorus
Aqueous acid is Mobile phase B, and detector is DAD detector, Detection wavelength 286nm, flow velocity 1.0ml/min, column temperature 35
DEG C, carry out gradient elution；The gradient elution process is same
Embodiment 1.
Solution is prepared and test operation is the same as embodiment 1.
Typical map is shown in that Figure 10, Figure 10 are the yihuang decoction finger-print that the embodiment of the present invention 5 measures.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of measuring method of Chinese medicine compound prescription yihuang decoction finger-print, which is characterized in that use high performance liquid chromatography, color
Spectral condition includes: that chromatographic column is octadecylsilane chemically bonded silica chromatographic column, using acetonitrile or methanol as mobile phase A, with formic acid water
Solution, acetic acid aqueous solution or phosphate aqueous solution are Mobile phase B, and detector is DAD detector, carry out gradient elution；
The gradient elution program are as follows: in 0-40min, the volume ratio of mobile phase A and Mobile phase B is from 5:95 at the uniform velocity gradual change to 30:
70；In 40-55min, the volume ratio of mobile phase A and Mobile phase B is from 30:70 at the uniform velocity gradual change to 95:5；In 55-60min, mobile phase
The volume ratio of A and Mobile phase B is from 95:5 at the uniform velocity gradual change to 5:95；In 60-65min, the volume ratio of mobile phase A and Mobile phase B is
5:95.
2. the measuring method of Chinese medicine compound prescription yihuang decoction finger-print according to claim 1, which is characterized in that the length of chromatographic column
Degree is 250mm, diameter 4.6mm；Preferably, the octadecylsilane chemically bonded silica partial size of chromatographic column is 2-10 μm；Preferably,
The octadecylsilane chemically bonded silica partial size of chromatographic column is 5 μm.
3. the measuring method of Chinese medicine compound prescription yihuang decoction finger-print according to claim 1 or claim 2, which is characterized in that formic acid water
The volume parts of solution, acetic acid aqueous solution or phosphate aqueous solution are 0.01-1.5%；Preferably, aqueous formic acid, acetic acid water
The volume fraction of solution or phosphate aqueous solution is 0.5%.
4. the measuring method of any one of -3 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that stream
Dynamic phase A is acetonitrile.
5. the measuring method of any one of -4 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that stream
Dynamic phase B is phosphate aqueous solution.
6. the measuring method of any one of -5 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that stream
Speed is 0.5-1.5ml/min；Preferably, flow velocity 1.0ml/min.
7. the measuring method of any one of -6 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that column
Temperature is 20-40 DEG C；Preferably, column temperature is 35 DEG C.
8. the measuring method of any one of -7 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that inspection
Survey wavelength is 280-290nm；Preferably, Detection wavelength 286nm；Preferably, sample volume is 8-12 μ L；Preferably, sample volume
For 10 μ L.
9. the measuring method of any one of -8 Chinese medicine compound prescription yihuang decoction finger-prints according to claim 1, which is characterized in that survey
Fixed specific steps are as follows: prepare test solution, control medicinal material solution, successively sample introduction, record chromatogram；Preferably, comparison medicine
Material is Chinese yam, Gorgon fruit, Cortex Phellodendri, semen plantaginis and gingko；Preferably, test solution preparation method are as follows: yihuang decoction dried cream powder is weighed,
Solubilizer ultrasound 40-50min, shakes up, and lets cool filtration and obtains test solution；Preferably, the preparation method of control medicinal material solution
Are as follows: control medicinal material dried cream powder is weighed, solubilizer ultrasound 40-50min shakes up, lets cool filtration and obtain control medicinal material solution.
10. the measuring method of Chinese medicine compound prescription yihuang decoction finger-print according to claim 9, which is characterized in that solvent is first
The volume ratio of the mixed liquor for the phosphate aqueous solution that pure and mild volume fraction is 0.1%, methanol and phosphate aqueous solution is 2-3:3-2；It is excellent
The volume ratio of selection of land, methanol and phosphate aqueous solution is 1:1.
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| CN114965809A (en) * | 2022-05-16 | 2022-08-30 | 海南葫芦娃药业集团股份有限公司 | Method for identifying effective components of cortex phellodendri chinensis in Yihuang decoction |
| CN114965809B (en) * | 2022-05-16 | 2024-03-05 | 海南葫芦娃药业集团股份有限公司 | Identification method of effective components of cortex phellodendri capable of being salted easily by Huang Shangzhong |
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