CN109825544A - A kind of scallop peptide and its production method - Google Patents
A kind of scallop peptide and its production method Download PDFInfo
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Abstract
The invention belongs to scallop peptide field, a kind of scallop peptide and its production method are disclosed.The production method of the scallop peptide includes by scallop through raw material pre-treatment, high efficiency compounded enzyme preparate digests under the conditions of not adjusting pH value, not controlling temperature, and inorganic nanofiltration ceramic membrane filter removal not readily dissolves the ingredient with the easy moisture absorption, high temperature high pressure process, concentration, dry powder processed.Do not need to adjust enzymatic hydrolysis pH value using method provided by the invention, do not need that controlled enzymatic hydrolysis temperature, enzymolysis time are short, enzyme additive amount is few, enzymolysis product molecular weight is low and concentrate, obtained scallop peptide powder water solubility is splendid and storage in be not easy moisture absorption agglomeration.
Description
Technical field
The present invention relates to scallop peptide field, in particular to a kind of scallop peptide and its production method.
Background technique
Scallop edge is the by-product generated in scallop process, has nutritional ingredient abundant and higher exploitation value
Value.However, this part resource is usually taken as production leftover bits and pieces and is dropped at present, only minority is utilized, and is such as processed
At fish and shrimp diet, soft canned food, fresh food etc..Therefore, the value of by-product both may be implemented again to the research of scallop edge
It utilizes, and nutrition product abundant can be provided for people.
CN106720914A discloses a kind of preparation method of scallop edge polypeptide dry powder, this method comprises: scallop edge is done
Dry, crushing, is added deionized water and alkali protease, is digested at suitable temperature and pH value, to enzyme after enzymatic hydrolysis is complete
It is inactivated, is centrifuged, takes supernatant, active carbon is added in supernatant and carries out defishying, is then centrifuged for, takes supernatant, most
Afterwards, by supernatant freeze-drying, powder processed.This method can make full use of the scallop edge abandoned in scallop process, and
The sensory issues that scallop edge fishy smell can be overcome too heavy are conducive to the large-scale promotion of scallop edge product.
CN105087736A discloses a kind of bay scallop polypeptide and its preparation method and application, this method comprises: to new
Fresh bay scallop carries out pre-treatment, grinding, ultrasonication, enzymatic hydrolysis, ultrafiltration and chromatography；Wherein, enzymatic hydrolysis needs first to use compound protein
Enzymatic treatment, then the bay scallop polypeptide that molecular weight is 700~790u is obtained with basic protein enzymatic treatment, then through ultrafiltration and chromatography.
By this method production bay scallop polypeptide, not only yield is high, purity is high, the growth inhibition examination through human hepatocarcinoma BEL-7402
It tests, it was demonstrated that gained bay scallop polypeptide has the function of very strong inhibition activity of tumor cells.
However, problems and disadvantages existing for above-mentioned two pieces application for a patent for invention are as follows: the enzymolysis efficiency of the enzyme of use is low, enzymatic hydrolysis
Time is long, and enzyme additive amount is more, needs to adjust optimum pH and optimum temperature is digested, complicated for operation, adjusts peak enzymolysis-ability pH
Value can bring a large amount of inorganic ions into, and control peak enzymolysis-ability temperature energy consumption is larger；The film core that uses of film filtering for organic material, intolerant to
Soda acid, non-refractory high pressure, flux is small, service life is short, and cleaning is inconvenient；The water solubility of prepared scallop peptide powder compared with
Difference is easy moisture absorption agglomeration in storage.
Summary of the invention
The purpose of the present invention is to provide one kind not to need to adjust enzymatic hydrolysis pH value, does not need controlled enzymatic hydrolysis temperature, enzymatic hydrolysis production
Object molecular weight is low and concentrates, is made that scallop peptide powder water solubility is splendid and be not easy in storage the scallop peptide of moisture absorption agglomeration
Production method and the scallop peptide that is produced by this method.
Specifically, the present invention provides a kind of production methods of scallop peptide, wherein method includes the following steps:
(1) raw material pre-treatment: by scallop decladding, taking scallop, and the water of 1~4 times of raw material weight, tissue are added after cleaning up
It smashs into homogenate to pieces, heats 10~30min at 80~100 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid；
(2) it digests: toward described to which the high efficiency compound enzyme that dosage is raw material weight 0.05~0.15% is added in enzymolysis liquid
Preparation digests 0.5~2h in the case where not adjusting pH value, the natural conditions for not controlling temperature after mixing, it is molten to obtain thick scallop peptide
Liquid；The high efficiency compounded enzyme preparate is by 20~25wt% compound protease, 20~25wt% alkali protease, 20~25wt%
Papain, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and 2~3wt% zymoexciter composition,
The zymoexciter contains reducing agent and bivalent metal ion；Mole of reducing agent and bivalent metal ion in the zymoexciter
Than for 1:(0.5~2)；
(3) inorganic nanofiltration ceramic membrane filter: molecular cut off is used to receive for the inorganic of 800Da the thick scallop peptide solution
Filter ceramic membrane is filtered, and gained permeate is high-purity scallop peptide solution；
(4) high temperature high pressure process: the high-purity scallop peptide solution is subjected to high temperature high pressure process, obtains treatment fluid；
(5) it is concentrated: the treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate；
(6) dry powder processed: the concentrate is spray-dried, scallop peptide powder is obtained.
Further, the molar ratio of reducing agent and bivalent metal ion is 1:(0.9~1.1 in the zymoexciter).
Further, the reducing agent is dithiothreitol (DTT) and/or beta -mercaptoethanol.
Further, the bivalent metal ion is Mg2+、Zn2+And Ca2+Mixture.
Further, Mg2+、Zn2+And Ca2+Molar ratio be (0.9~1.1): (0.9~1.1): 1.
Further, the filter core of the inorganic nanofiltration ceramic membrane is using in siliceous, aluminum, magnesia, sa and simatic
Any one material be made.
Further, the condition of the high temperature high pressure process include temperature be 110~130 DEG C, pressure be 0.1~
0.3MPa, time are 5~20min.
In addition, the present invention also provides the scallop peptides produced by the above method.
Key means of the invention first is that treating enzymatic hydrolysis using heat treatment and specific high efficiency compounded enzyme preparate
Liquid is digested, wherein the heat treatment enables to scallop albuminous degeneration, so that being easier to be digested, improves enzymatic hydrolysis effect
Rate, the high efficiency compounded enzyme preparate is by 20~25wt% compound protease, 20~25wt% alkali protease, 20~25wt%
Papain, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and the (reduction of 2~3wt% zymoexciter
The mixture of agent and bivalent metal ion) composition, this specific compounded enzyme preparate is containing there are many can be directed to scallop meat albumen
Endo protease, exoproteinase and the breaking cell wall enzyme digested efficiently specificity can digest scallop meat albumen, and enzyme swashs
Reducing agent contained in agent living and bivalent metal ion can activate the enzyme activity of these types of protease, will above several albumen
The enzyme preparation that enzyme compounds formation according to the above ratio under the action of reducing agent and bivalent metal ion has extremely strong scallop
Proteolysis efficiency.The scallop meat albumen after heated is digested using the above highly efficiency compositional enzyme preparation, is not adjusting pH
Value does not control under the natural conditions of temperature, and thoroughly enzymatic hydrolysis scallop meat albumen can be realized under the premise of adding a small amount of protease
Purpose, enzymolysis product molecular weight it is small and concentrate, enzymolysis product free aminoacid content is low, protein molecular weight be 1000Da with
Under ratio reached 100%, the scallop protein peptides ratio between 200~1000Da has reached 95% or more.In addition, a side
Face is not needed to be adjusted peak enzymolysis-ability pH value using soda acid, does not bring inorganic ions into enzymolysis process, subsequent not need again
It takes nanofiltration membrane to remove inorganic ions, simplifies processing step；On the other hand, it in enzymolysis process, does not need to adjust most suitable yet
Hydrolysis temperature, compared with the prior art, not only easy to operate but also saving energy consumption.
To sum up, beneficial effects of the present invention are as follows:
(1) using the combination technique means of heat treatment and high efficiency compounded enzyme preparate enzymatic hydrolysis, enzymatic hydrolysis fan is significantly improved
The enzymolysis efficiency of shellfish meat albumen, so that adding a small amount of egg in the case where not adjusting optimum pH, not controlling the natural conditions of optimum temperature
White enzyme digests the short period, can thoroughly digest scallop meat albumen, enzymolysis product molecular weight is small and concentrates, and enzymolysis product is free
Amino acid content is low, and protein molecular weight is that 1000Da ratio below reaches 100%, and the ratio between 200~1000Da reaches
To 95% or more.In addition, do not need to adjust peak enzymolysis-ability pH value using soda acid in enzymolysis process, do not bring into or not inorganic from
Son, it is subsequent not need that nanofiltration membrane is taken to remove inorganic ions again, simplify processing step；In enzymolysis process, do not need to adjust yet
Peak enzymolysis-ability temperature, compared with the prior art, not only easy to operate but also saving energy consumption.
(2) technological means that inorganic nanofiltration ceramic membrane filter is used after enzymatic hydrolysis removes the substance that not readily dissolves and easily
The carbohydrate content of the moisture absorption improves the water solubility of finally obtained scallop peptide finished product, and is not easy the moisture absorption during finished stock storage
Agglomeration.Inorganic nanofiltration ceramic membrane compared with organic nanofiltration membrane, have organic solvent-resistant, high temperature resistant, acid and alkali-resistance, high mechanical strength,
The advantage that flux is big and the service life is long.The present invention need to only use a nanofiltration membrane treatment, than both needing using big point of ultrafiltration membrane retention
Sub- substance needs the prior art using nanofiltration membrane desalination and concentration to compare again, significantly simplifies processing step, and film process
Effect is different.
(3) gained scallop peptide solution is used into high temperature high pressure process after ceramic membrane filter, can further mentioned in this way
The water solubility of high finally obtained scallop peptide finished product, finished product is finer and smoother, is highly soluble in water.
Specific embodiment
The present invention described in detail below.
In the present invention, term " scallop peptide " refers to the protein peptides produced using scallop as raw material.
In the present invention, initial feed scallop can be fresh scallop, or frost scallop obtains after thawing.
In the present invention, the effect of the raw material pre-treatment is sufficiently to extract the protein in scallop and make scallop egg
Leucismus improves enzymolysis efficiency to be more advantageous to subsequent enzymatic hydrolysis.
In the present invention, the enzymatic hydrolysis carries out under the conditions of not adjusting pH value, not controlling temperature.After the enzymatic hydrolysis, fan
Free aminoacid content is low, protein molecular weight is for can be degraded into after shellfish meat albumen and heated denaturation to enzymolysis liquid
The ratio of 1000Da has reached the enzymolysis product of 100% (having reached 95% or more between the scallop peptide ratio of 200~1000Da).
Wherein, protease contained in the high efficiency compounded enzyme preparate can pass through commercially available acquisition.For example, the compound protease
It can be the compound protease purchased from Novozymes Company.The alkali protease can be for purchased from Nanning Pang Bo bioengineering
Co., Ltd is originated from the resulting alkali protease of the lichen bacillus ferments.The papain can be for purchased from Guang Xinan
The pawpaw that is originated from of Ning Pangbo bioengineering Co., Ltd naturally extracts resulting papain.The flavor protease can be
The resulting flavor protease of aspergillus oryzae fermentation is originated from purchased from Nanning Pang Bo bioengineering Co., Ltd.The animal protein
Hydrolase can be the animal proteolytic enzyme from complex enzyme purchased from Nanning Pang Bo bioengineering Co., Ltd.
The zymoexciter contains reducing agent and bivalent metal ion.Wherein, the reducing agent is preferably dithiothreitol (DTT)
And/or beta -mercaptoethanol.The bivalent metal ion is preferably selected from Mg2+、Zn2+And Ca2+At least one of, more preferably Mg2 +、Zn2+And Ca2+Mixture, most preferably Mg2+、Zn2+And Ca2+According to molar ratio (0.9~1.1): (0.9~1.1): 1 compounding
Obtained mixture.The bivalent metal ion can exist in the form of the salt containing the above metal ion.It is particularly preferred that
The zymoexciter is by reducing agent and bivalent metal ion and can be with the anion group of the bivalent metal ion forming salt
At.In addition, the molar ratio of reducing agent and bivalent metal ion is particularly preferably 1:(0.9~1.1 in the zymoexciter).
In the present invention, the effect of the ceramic membrane filter be remove the carbohydrate of the ingredient not readily dissolved and the easy moisture absorption at
Point, the water solubility of finally obtained scallop peptide finished product is improved, and moisture absorption agglomeration is not easy during finished stock storage.Inorganic nanofiltration
Ceramic membrane is compared with organic nanofiltration membrane, and with organic solvent-resistant, high temperature resistant, acid and alkali-resistance, high mechanical strength, flux is big and the service life is long
Advantage.The filter core of the inorganic nanofiltration ceramic membrane preferably uses any in siliceous, aluminum, magnesia, sa and simatic
A kind of material is made.The molecular cut off of the inorganic nanofiltration ceramic membrane is 800Da, when molecular cut off is more than 800Da
When, the carbohydrate content for removing the ingredient not readily dissolved and the easy moisture absorption is not thorough, when molecular cut off is less than 800Da, scallop peptide
Loss late is high.
In the present invention, the effect of the high temperature high pressure process is the water for further increasing finally obtained scallop peptide finished product
Solubility easily dissolves so that finished product is finer and smoother, and high-purity scallop peptide solution is after high temperature high pressure process, can also be with
Play the role of thoroughly sterilizing and eliminating hypersensitive.The high temperature high pressure process preferably temperature not less than 110 DEG C, pressure it is not low
It is carried out under conditions of 0.1MPa, it is highly preferred that it is 110~130 DEG C that the condition of the high temperature high pressure process, which includes temperature, pressure
For 0.1~0.3MPa, the time is 5~20min.In the present invention, the pressure refers both to gauge pressure.
In the present invention, the effect that the heating under reduced pressure is concentrated is will be in the high-purity scallop peptide after high temperature high pressure process
Moist part or all removal.
In the present invention, the effect of the spray drying is that concentrate is processed into powder.Wherein, the spray drying
Temperature can be 110~140 DEG C.
In addition, can also be packed, after formation scallop peptide powder to be spray dried to sell outside.
Below with reference to embodiment, the content of the present invention will be explained in more detail.It should be appreciated that the embodiment of the present invention is example
Property, it is intended to it is used to explain the present invention, and is not considered as limiting the invention.Particular technique or item are not specified in embodiment
Part person described technology or conditions or carries out according to the literature in the art according to product description.Agents useful for same or instrument
Production firm person is not specified in device, and being can be with conventional products that are commercially available.
In following embodiment and comparative example, compound protease, alkali protease, papain, flavor protease, animal
The source of proteolytic enzyme and manufacturing enterprise are as shown in table 1 below:
Table 1
| The title of enzyme | Source | Manufacturing enterprise |
| Compound protease | Complex enzyme | Novozymes Company |
| Alkali protease | The lichen bacillus ferments | Nanning Pang Bo bioengineering Co., Ltd |
| Papain | Pawpaw is naturally extracted | Nanning Pang Bo bioengineering Co., Ltd |
| Flavor protease | Aspergillus oryzae fermentation | Nanning Pang Bo bioengineering Co., Ltd |
| Animal proteolytic enzyme | Complex enzyme | Nanning Pang Bo bioengineering Co., Ltd |
Embodiment 1
(1) raw material pre-treatment: by scallop decladding, taking scallop, and the water of 1 times of raw material weight is added after cleaning up, and tissue is smash
It is broken into homogenate, heats 10min at 100 DEG C later, then is cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested under the conditions of not adjusting pH value, not controlling temperature after uniformly, obtains thick scallop peptide solution.The high efficiency compound enzyme system
Agent is by 20wt% compound protease, 25wt% alkali protease, 20wt% papain, 25wt% flavor protease, 7wt%
Animal proteolytic enzyme and 3wt% zymoexciter composition, wherein the zymoexciter is by reducing agent dithiothreitol and divalent gold
Belong to ION Mg2+、Zn2+、Ca2+The molar ratio of compounding composition, the reducing agent and bivalent metal ion is 1:0.5, Mg2+、Zn2+With
Ca2+Molar ratio be 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: molecular cut off is used to receive for the inorganic of 800Da the thick scallop peptide solution
Filter ceramic membrane is filtered, and gained permeate is high-purity scallop peptide solution.
(4) high temperature high pressure process: by the high-purity scallop peptide solution in the item that temperature is 130 DEG C, pressure is 0.1MPa
High temperature high pressure process 0.5min under part, obtains treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains scallop peptide finished product after packaging.
Embodiment 2
(1) raw material pre-treatment: by scallop decladding, taking scallop, and the water of 3 times of raw materials weight is added after cleaning up, and tissue is smash
It is broken into homogenate, heats 20min at 90 DEG C later, then is cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested under the conditions of not adjusting pH value, not controlling temperature after uniformly, obtains thick scallop peptide solution.The high efficiency compound enzyme system
Agent is by 22.5wt% compound protease, 22.5wt% alkali protease, 22.5wt% papain, 22.5wt% flavor albumen
Enzyme, 7.5wt% animal proteolytic enzyme and 2.5wt% zymoexciter composition, wherein the zymoexciter is revived by two sulphur of reducing agent
Sugar alcohol, beta -mercaptoethanol and bivalent metal ion Mg2+、Zn2+、Ca2+Composition, the reducing agent dithiothreitol and β-sulfydryl
The molar ratio of ethyl alcohol is 1:1, and the molar ratio of the reducing agent and bivalent metal ion is 1:1, Mg2+、Zn2+And Ca2+Molar ratio
For 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: molecular cut off is used to receive for the inorganic of 800Da the thick scallop peptide solution
Filter ceramic membrane is filtered, and gained permeate is high-purity scallop peptide solution.
(4) high temperature high pressure process: by the high-purity scallop peptide solution in the item that temperature is 120 DEG C, pressure is 0.2MPa
High temperature high pressure process 20min under part, obtains treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains scallop peptide finished product after packaging.
Embodiment 3
(1) raw material pre-treatment: by scallop decladding, taking scallop, and the water of 4 times of raw materials weight is added after cleaning up, and tissue is smash
It is broken into homogenate, heats 30min at 80 DEG C later, then is cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested under the conditions of not adjusting pH value, not controlling temperature after uniformly, obtains thick scallop peptide solution.The high efficiency compound enzyme system
Agent is by 25wt% compound protease, 20wt% alkali protease, 25wt% papain, 20wt% flavor protease, 8wt%
Animal proteolytic enzyme and 2wt% zymoexciter composition, wherein the zymoexciter is by reducing agent beta -mercaptoethanol and divalent gold
Belong to ION Mg2+、Zn2+、Ca2+The molar ratio of composition, the reducing agent and bivalent metal ion is 1:2, Mg2+、Zn2+And Ca2+'s
Molar ratio is 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: molecular cut off is used to receive for the inorganic of 800Da the thick scallop peptide solution
Filter ceramic membrane is filtered, and gained permeate is high-purity scallop peptide solution.
(4) high temperature high pressure process: by the high-purity scallop peptide solution in the item that temperature is 130 DEG C, pressure is 0.1MPa
High temperature high pressure process 10min under part, obtains treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains scallop peptide finished product after packaging.
Comparative example 1
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compounded enzyme preparate
In compound protease using identical weight percentage composition animal proteolytic enzyme substitute, remaining is same as Example 1, obtains
Reference scallop peptide finished product.
Comparative example 2
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compounded enzyme preparate
In alkali protease using identical weight percentage composition animal proteolytic enzyme substitute, remaining is same as Example 1, obtains
Reference scallop peptide finished product.
Comparative example 3
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compounded enzyme preparate
In papain using identical weight percentage composition animal proteolytic enzyme substitute, remaining is same as Example 1, obtains
Reference scallop peptide finished product.
Comparative example 4
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compounded enzyme preparate
In flavor protease using identical weight percentage composition animal proteolytic enzyme substitute, remaining is same as Example 1, obtains
Reference scallop peptide finished product.
Comparative example 5
Scallop tripe peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Animal proteolytic enzyme in agent uses the basic protein enzymes extraction of identical weight percentage composition, remaining is same as Example 1, obtains
To reference scallop tripe peptide finished product.
Comparative example 6
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, in high efficiency compounded enzyme preparate
Contained zymoexciter is completely by bivalent metal ion Mg2+、Zn2+、Ca2+According to molar ratio be 1:1:1 compounding composition, remaining with
Embodiment 1 is identical, obtains reference scallop peptide finished product.
Comparative example 7
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, in high efficiency compounded enzyme preparate
Contained zymoexciter is made of dithiothreitol (DTT) completely, remaining is same as Example 1, obtains reference scallop peptide finished product.
Comparative example 8
Scallop peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compounded enzyme preparate
Contained in zymoexciter using identical weight percentage composition animal proteolytic enzyme substitute, remaining is same as Example 1, obtains
To reference scallop peptide finished product.
Test case
(1) high efficiency compounded enzyme preparate hydrolysis result detects:
In Examples 1 to 3 and comparative example 1~8, scallop meat protein is under the action of high efficiency compounded enzyme preparate not
PH is adjusted, does not control and digests under conditions of temperature, in order to assess hydrolysis result, after enzymatic hydrolysis, using " the People's Republic of China (PRC)
Standard GB/T/T 22729-2008 " method in appendix A carries out enzymolysis product detection, and the results are shown in Table 2:
Table 2
As shown in Table 2, the enzymolysis product molecular weight of Examples 1 to 3 is small and concentrates, enzymolysis product free amino acid
Content is low (ratio less than 200Da is less than 5%), and ratio of the protein molecular weight less than 1000Da reaches 100%, between 200
The ratio of~1000Da has reached 95% or more, and enzymolysis product molecular weight is substantially better than comparative example 1~8.It is possible thereby to illustrate, adopt
With the combination technique means of heat treatment provided by the invention and high efficiency compounded enzyme preparate enzymatic hydrolysis, enzymatic hydrolysis fan can be significantly improved
The enzymolysis efficiency of shellfish meat protein, so that addition is a small amount of in the case where not adjusting optimum pH, not controlling the natural conditions of optimum temperature
Protease digests the short period, can thoroughly digest scallop meat protein.
(2) scallop peptide finished product physical and chemical index detects:
Physical and chemical index detection is carried out to the scallop peptide finished powder that Examples 1 to 3 obtains, wherein total nitrogen detection method ginseng
It is carried out according to GB5009.5, ash content detection method is carried out referring to GB 5009.4, as a result as shown in table 3 below:
Table 3
| Project | Total nitrogen (in terms of butt) (g/100g) | Ash content (g/100g) |
| Embodiment 1 | 16.2 | 0.7 |
| Embodiment 2 | 15.7 | 0.8 |
| Embodiment 3 | 15.8 | 0.9 |
As shown in Table 3, the total nitrogen for the scallop peptide finished product that Examples 1 to 3 obtains, ash content index are lower, explanation
Using scallop peptide purity is high obtained by method provided by the invention, quality is good, and especially content of ashes only has 0.7~0.9g/100g.
Illustrate to digest using this specific high efficiency compounded enzyme preparate of the present invention in the case where not adjusting pH value condition, not need with soda acid tune
Finished product content of ashes can be greatly reduced so that production process will not introduce inorganic ions by saving pH value.
(3) light transmittance of the water solubility of scallop peptide finished product and scallop peptide solution detects:
The scallop peptide finished powder 8g that Examples 1 to 3 obtains is poured into different cups respectively, with 60 DEG C of 300mL or so temperature
Boiled water reconstitutes, and i.e. all dissolutions, obtain scallop peptide lysate in the 1s time, visually observes lysate clear, without any heavy
It forms sediment.The light transmittance of several scallop peptide lysates more than being detected at 620nm using ultraviolet specrophotometer, as a result such as the following table 4 institute
Show:
Table 4
| Project | Light transmittance (%) |
| Embodiment 1 | 97 |
| Embodiment 2 | 92 |
| Embodiment 3 | 94 |
As shown in Table 4, using the technological means of inorganic nanofiltration ceramic membrane filter, the substance not readily dissolved is removed,
Again using the technological means of the high temperature high pressure process of peptide solution, the solubility property of finished product is further improved, so that the fan obtained
Shellfish peptide finished product is highly soluble in that water, water solubility are splendid, lysate clear after being dissolved in water, high without any precipitating, light transmittance,
When consumer brews this product, without stirring for it is the technical effect that the prior art is not achieved that convenient, edible experience is good.
(4) the shelf life test of scallop peptide finished product:
The scallop peptide finished powder 8g that Examples 1 to 3 obtains is poured into packaging bag respectively, room temperature opening is placed in interior
On Display Rack, visually observes finished product and the time agglomerated occur, as a result as shown in table 5 below:
Table 5
| Project | There is the time (day) agglomerated |
| Embodiment 1 | 55 |
| Embodiment 2 | 52 |
| Embodiment 3 | 56 |
The scallop peptide of prior art production is easy moisture absorption agglomeration, influences the sale of product in storage.By table 5
As a result it is found that the present invention uses the technological means of inorganic nanofiltration ceramic membrane filter, the carbohydrate content of the easy moisture absorption is removed, so that finished product
It is not easy moisture absorption agglomeration in storage, is easy to store, is the technical effect that the prior art is not achieved.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (8)
1. a kind of production method of scallop peptide, which is characterized in that method includes the following steps:
(1) raw material pre-treatment: by scallop decladding, taking scallop, and the water of 1~4 times of raw material weight, tissue mashing are added after cleaning up
At homogenate, 10~30min is heated at 80~100 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid；
(2) it digests: toward described to which the high efficiency compounded enzyme preparate that dosage is raw material weight 0.05~0.15% is added in enzymolysis liquid,
0.5~2h is digested in the case where not adjusting pH value, the natural conditions for not controlling temperature after mixing, obtains thick scallop peptide solution；Institute
High efficiency compounded enzyme preparate is stated by 20~25wt% compound protease, 20~25wt% alkali protease, 20~25wt% pawpaw
Protease, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and 2~3wt% zymoexciter composition, it is described
Zymoexciter contains reducing agent and bivalent metal ion；The molar ratio of reducing agent and bivalent metal ion is in the zymoexciter
1:(0.5~2)；
(3) inorganic nanofiltration ceramic membrane filter: molecular cut off is used to make pottery for the inorganic nanofiltration of 800Da the thick scallop peptide solution
Porcelain film is filtered, and gained permeate is high-purity scallop peptide solution；
(4) high temperature high pressure process: the high-purity scallop peptide solution is subjected to high temperature high pressure process, obtains treatment fluid；
(5) it is concentrated: the treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate；
(6) dry powder processed: the concentrate is spray-dried, scallop peptide powder is obtained.
2. the production method of scallop peptide according to claim 1, which is characterized in that reducing agent and two in the zymoexciter
The molar ratio of valence metal ion is 1:(0.9~1.1).
3. the production method of scallop peptide according to claim 1, which is characterized in that the reducing agent is dithiothreitol (DTT)
And/or beta -mercaptoethanol.
4. the production method of scallop peptide according to claim 1, which is characterized in that the bivalent metal ion is Mg2+、
Zn2+And Ca2+Mixture.
5. the production method of scallop peptide according to claim 4, which is characterized in that Mg2+、Zn2+And Ca2+Molar ratio be
(0.9~1.1): (0.9~1.1): 1.
6. the production method of scallop peptide described according to claim 1~any one of 5, which is characterized in that described inorganic to receive
The filter core of filter ceramic membrane is made using any one material in siliceous, aluminum, magnesia, sa and simatic.
7. the production method of scallop peptide described according to claim 1~any one of 5, which is characterized in that the high temperature is high
The condition of pressure processing includes that temperature is 110~130 DEG C, and pressure is 0.1~0.3MPa, and the time is 5~20min.
8. the scallop peptide that the method as described in any one of claim 1~7 produces.
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