CN109820100A - A kind of preparation method of the alfalfa ensilage leavening and preparation method thereof for improving crude protein content, alfalfa ensilage - Google Patents
A kind of preparation method of the alfalfa ensilage leavening and preparation method thereof for improving crude protein content, alfalfa ensilage Download PDFInfo
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Abstract
The present invention provides the preparation methods of a kind of alfalfa ensilage leavening for improving crude protein content and preparation method thereof, alfalfa ensilage, belong to field of feed.The alfalfa ensilage leavening includes lactobacillus plantarum CICC20765 and Pediococcus pentosaceus CICC22737.Leavening provided by the invention can effectively improve the content of crude protein in alfalfa ensilage, embodiment the result shows that: lactobacillus plantarum CICC20765 and Pediococcus pentosaceus CICC22737 arrange in pairs or groups, compared to control group 1~6, crude protein content improves 5.4%~12.25%.
Description
Technical field
The invention belongs to field of feed, and in particular to it is a kind of improve crude protein content alfalfa ensilage leavening and its
Preparation method, the preparation method of alfalfa ensilage.
Background technique
Alfalfa (Medicago sativa) is widely used high-quality protein class herbage in milk cattle cultivating, is known as
King of Pasture.Often is overlapped with rainy season in the more region clover harvest of Summer Rainfall in China weather category rain heat same season, lose compared with
Greatly.Ensiling is difficult preferred method of harvesting and storing in solution clover rainy season as one of the effective means for promoting feeding value of forage.Lucerne
Mu ensiling (alfalfa silage), soft and succulency, palatability are good and digestibility is high, are convenient for long-term preservation.
Protein plays an important role to the growth and breeding of animal, and protein content is evaluation feed nutritive value
Important indicator.
Summary of the invention
In view of this, the present invention provides a kind of alfalfa ensilage leavening and preparation method thereof for improving crude protein content,
The preparation method of alfalfa ensilage can effectively improve the content of crude protein in alfalfa ensilage, increase the nutriture value of alfalfa ensilage
Value.
To solve the above-mentioned problems, the present invention provides following technical schemes:
The present invention provides a kind of alfalfa ensilage leavenings for improving crude protein content, including lactobacillus plantarum
CICC20765 and Pediococcus pentosaceus CICC22737.
The present invention provides a kind of preparation methods of alfalfa ensilage leavening described in above scheme, including by plant cream bar
Bacterium CICC20765 and Pediococcus pentosaceus CICC22737 ferment respectively, will obtain lactobacillus plantarum CICC20765 fermentation liquid
It is mixed with Pediococcus pentosaceus CICC22737 fermentation liquid, obtains alfalfa ensilage leavening.
Preferably, the quality of the lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid
Than being 0.9~1.1: 0.9~1.1；The lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation
The viable count of liquid stands alone as 1.0 × 108~1.0 × 1010CFU/g。
Preferably, the method for the fermentation includes: by lactobacillus plantarum CICC20765 bacterium solution and Pediococcus pentosaceus
CICC22737 bacterium solution is inoculated into respectively in liquid state fermentation culture medium, at 30~40 DEG C, under conditions of 80~120rpm ferment 20~
28h obtains lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid；The liquid state fermentation culture
Base takes water as a solvent, the component including following concentration: peptone 12g/L, powdered beef 12g/L, yeast powder 6g/L, glucose 24g/
It L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L, sodium acetate 5g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L and spits
Warm 5mL/L, the pH value of the liquid state fermentation culture medium are 5.8~6.0.
Preferably, the viable count of the lactobacillus plantarum CICC20765 bacterium solution is 1.0 × 108~× 1.0 × 1010CFU/
g；The viable count of the Pediococcus pentosaceus CICC22737 bacterium solution is 1.0 × 108~× 1.0 × 1010CFU/g。
Preferably, the inoculum concentration stands alone as the 5%~15% of liquid state fermentation total weight of medium.
The present invention provides a kind of preparation methods of alfalfa ensilage, include the following steps:
1) clover is mixed with alfalfa ensilage leavening described in above scheme, obtains total mixture；
2) total mixture of the step 1) is sealed 40~50d of fermentation, obtains alfalfa ensilage.
Preferably, the mass volume ratio of clover and alfalfa ensilage leavening is 1Kg: 1.4~1.6mL in the step 1).
It preferably, further include being diluted alfalfa ensilage leavening before being mixed described in step 1)；Described diluted times
Number is 3~5 times.
Preferably, the temperature being sealed by fermentation in the step 2) is 20~30 DEG C.
The present invention provides a kind of alfalfa ensilage leavenings and preparation method thereof for improving crude protein content, alfalfa ensilage
Preparation method.The alfalfa ensilage leavening includes lactobacillus plantarum CICC20765 and Pediococcus pentosaceus CICC22737.This hair
In bright, lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid are added simultaneously in ensilage
It ferments, breaks down proteins can be inhibited, while using the various Amino acid synthesis mycoproteins in feed, improving ensiling and raising
The crude protein content of material.Embodiment is the result shows that lactobacillus plantarum CICC20765 and Pediococcus pentosaceus CICC22737 collocation, phase
5.4%~12.25% is improved compared with 1~6 crude protein content of control group.
Specific embodiment
The present invention provides a kind of alfalfa ensilage leavenings for improving crude protein content, including lactobacillus plantarum
CICC20765 and Pediococcus pentosaceus CICC22737.
In the present invention, the lactobacillus plantarum CICC20765 purchase is from the management of Chinese industrial Microbiological Culture Collection
The heart.In the present invention, the Pediococcus pentosaceus CICC22737 is bought from Chinese industrial Microbiological Culture Collection administrative center.
The present invention provides a kind of preparation methods of alfalfa ensilage leavening described in above scheme, including by plant cream bar
Bacterium CICC20765 and Pediococcus pentosaceus CICC22737 ferment respectively, will obtain lactobacillus plantarum CICC20765 fermentation liquid
It is mixed with Pediococcus pentosaceus CICC22737 fermentation liquid, obtains alfalfa ensilage leavening.
In the present invention, the lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid
Mass ratio is preferably 0.9~1.1: 0.9~1.1, and more preferably 1: 1.In the present invention, the lactobacillus plantarum CICC20765
The viable count of fermentation liquid and Pediococcus pentosaceus CICC22737 are independently preferably 1.0 × 108~× 1.0 × 1010CFU/g, more preferably
It is 1.0 × 109CFU/g。
In the present invention, the method for the fermentation is preferred are as follows: by lactobacillus plantarum CICC20765 bacterium solution and Pediococcus pentosaceus
CICC22737 bacterium solution is inoculated into respectively in liquid state fermentation culture medium, at 30~40 DEG C, under conditions of 80~120rpm ferment 20~
28h respectively obtains lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid；The liquid state fermentation
Culture medium takes water as a solvent, the component including following concentration: peptone 12g/L, powdered beef 12g/L, yeast powder 6g/L, glucose
24g/L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L, sodium acetate 5g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L
With tween 5mL/L.In the present invention, the pH value of the liquid state fermentation culture medium is preferably 5.8~6.0, and more preferably 5.9.This
Invention is not particularly limited the source of each component in the liquid state fermentation culture medium, is using this field conventional commercial product
It can.
In the present invention, the amount of the inoculation of the lactobacillus plantarum CICC20765 bacterium solution is preferably liquid state fermentation culture medium
The 5%~15% of total weight, more preferably 10%.In the present invention, the viable count of the lactobacillus plantarum CICC20765 bacterium solution
Preferably 1.0 × 108~× 1.0 × 1010CFU/g, more preferably 1.0 × 109CFU/g.In the present invention, the plant cream bar
The preparation method of bacterium CICC20765 bacterium solution preferably includes that lactobacillus plantarum CICC20765 is successively activated to and expanded culture
It obtains.In the present invention, the mode of the activation is preferably by lactobacillus plantarum CICC20765 in MRS solid slope culture medium
Upper scribing line, by scribed culture medium in 35 DEG C of cultures to growing bacterium colony.In the present invention, the number of the activation is preferably 2
It is secondary.In the present invention, the single colonie after activation is preferably inoculated in MRS fluid nutrient medium by the mode for expanding culture,
At 35 DEG C, 120rpm is overnight, obtains lactobacillus plantarum CICC20765 bacterium solution.
In the present invention, the amount of the inoculation of the Pediococcus pentosaceus CICC22737 bacterium solution is preferably liquid state fermentation culture medium
The 5%~10% of total weight, preferably 10%.In the present invention, the viable count of the Pediococcus pentosaceus CICC22737 bacterium solution is excellent
It is selected as 1.0 × 108~× 1.0 × 1010CFU/g, more preferably 1.0 × 109CFU/g.In the present invention, the Pediococcus pentosaceus
The preparation method of CICC22737 bacterium solution preferably includes that Pediococcus pentosaceus CICC22737 is successively activated and expanded to cultivate
It arrives.In the present invention, the mode of the activation is preferably by Pediococcus pentosaceus CICC22737 in MRS solid slope culture medium
Scribing line cultivates scribed culture medium at 35 DEG C to growing bacterium colony.In the present invention, the number of the activation is preferably 2
It is secondary.In the present invention, the single colonie after activation is preferably inoculated in MRS fluid nutrient medium by the mode for expanding culture,
At 35 DEG C, 120rpm is overnight, obtains Pediococcus pentosaceus CICC22737 bacterium solution.
In the present invention, the temperature of the fermentation is 30~40 DEG C, preferably 35 DEG C；The mixing speed of the fermentation is 80
~120rpm, preferably 100rpm；The time of the fermentation is 20~28h, preferably for 24 hours.
In the present invention, lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus are added in ensilage simultaneously
CICC22737 fermentation liquid ferments, and can inhibit breaks down proteins, while utilizing the various Amino acid synthesis thallus in feed
Albumen improves the crude protein content of ensilage.
The present invention provides a kind of preparation methods of alfalfa ensilage, include the following steps:
1) clover is mixed with alfalfa ensilage leavening described in above scheme, obtains total mixture；
2) total mixture of the step 1) is sealed 40~50d of fermentation, obtains alfalfa ensilage.
The present invention mixes clover with alfalfa ensilage leavening, obtains total mixture.In the present invention, before the mixing preferably
Clover is cut into the clover section of 2~5cm, more preferably 4cm.In the present invention, the clover and alfalfa ensilage leavening
Mass volume ratio is preferably 1Kg: 1.4~1.6mL, and more preferably 1Kg: 1.5mL.
The present invention is not particularly limited the source of clover, using this field routine clover.In the embodiment of the present invention
The polar bear kind provided with Dan Nong seeds company, " the beach saline land greening soil of agricultural resource and Environmental Research Institute in Tianjin
Earth improvement scientific research and testing base " plantation obtains.
In the present invention, preferably alfalfa ensilage leavening is diluted before the mixing；The diluted multiple is preferred
It is 3~5 times, more preferably 4 times.The dilution is preferably water with dilution.In the present invention, the mixed mode is preferably
Alfalfa ensilage leavening is sprayed on clover.
After obtaining total mixture, the total mixture is preferably sealed 40~50d of fermentation by the present invention, obtains alfalfa ensilage.
In the present invention, the mode of the sealing is preferably sealed using vinyon.Preferred discharge air before sealing.?
In the present invention, the temperature of the sealing and fermenting is preferably 20~30 DEG C, and more preferably 25 DEG C.The time of the sealing and fermenting is preferred
For 45d.
In order to further illustrate the present invention, technical solution provided by the invention is retouched in detail below with reference to embodiment
It states, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Lactobacillus plantarum CICC20765 and Pediococcus pentosaceus are taken out from cryopreservation tube with sterilized oese
CICC22737 crosses respectively on MRS solid slope, and scribed MRS solid medium is placed in 35 DEG C of incubators and is cultivated
Bacterium colony out, picking single colonie is connected in 35 DEG C in MRS fluid nutrient medium after activating 2 times, and 120rpm/min is overnight, obtains plant cream
Bacillus CICC20765 bacterium solution and Pediococcus pentosaceus CICC22737 bacterium solution are spare.
Obtained lactobacillus plantarum CICC20765 bacterium solution and Pediococcus pentosaceus CICC22737 bacterium solution are separately adjusted to angularly bacterium
Concentration is 1.0 × 109After CFU/g, (inoculum concentration be the 10% of fluid nutrient medium weight) into fluid nutrient medium (egg is inoculated with respectively
White peptone 12g/L, powdered beef 12g/L, yeast powder 6g/L, glucose 24g/L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L,
Sodium acetate 5g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, tween 5mL, distilled water constant volume to 1L；It is prepared by above-mentioned formula
After good liquid culture medium, pH value is adjusted to 5.9), is cultivated for 24 hours at 35 DEG C, under conditions of 100rpm, obtains lactobacillus plantarum
CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid.
The test polar bear kind that clover is that Dan Nong seeds company provides, planting area is Agriculture In Tianjin resource and ring
" beach saline land green soil improves scientific research and testing base " of border research institute, mu application rate 1.2Kg.Clover flower at the beginning of first batch
Phase cradles clover and is cut to the clover of 2~5cm with hay cutter after sunning for 24 hours (crude protein content 29.13 ± 0.75%)
Section, for making alfalfa ensilage.
Embodiment 2
The lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid that Example 1 obtains,
It is mixed according to 1: 1 mass ratio, obtains alfalfa ensilage leavening.
Above-mentioned alfalfa ensilage leavening is sprayed at clover section upper (clover section and alfalfa ensilage by the clover section of Example 1
The mass volume ratio of leavening is 1Kg: 1.5mL, first will be after 4 times of dilution agent of fermentation for the homogeneity for guaranteeing alfalfa ensilage addition
It is sprayed onto clover section again), it is packed into polyethylene plastic bag (25cm × 35cm), every bag of 300g is arranged 3 repetitions, uses sealing machine
It is evacuated and is sealed simultaneously, bag is opened after 25 DEG C of fermentation 45d, analyze alfalfa ensilage nutritional ingredient.
Embodiment 3
The lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid that Example 1 obtains,
It is mixed according to 0.9: 1.1 mass ratio, obtains alfalfa ensilage leavening.
Above-mentioned alfalfa ensilage leavening is sprayed at clover section upper (clover section and alfalfa ensilage by the clover section of Example 1
The mass volume ratio of leavening is 1Kg: 1.5mL, first will be after 3 times of dilution agent of fermentation for the homogeneity for guaranteeing alfalfa ensilage addition
It is sprayed onto clover section again), it is packed into polyethylene plastic bag (25cm × 35cm), every bag of 300g is arranged 3 repetitions, uses sealing machine
It is evacuated and is sealed simultaneously, bag is opened after 20 DEG C of fermentation 50d, analyze alfalfa ensilage nutritional ingredient.
Embodiment 4
The lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid that Example 1 obtains,
It is mixed according to 1.1: 1 mass ratio, obtains alfalfa ensilage leavening.
Above-mentioned alfalfa ensilage leavening is sprayed at clover section upper (clover section and alfalfa ensilage by the clover section of Example 1
The mass volume ratio of leavening is 1Kg: 1.4mL, first will be after 5 times of dilution agent of fermentation for the homogeneity for guaranteeing alfalfa ensilage addition
It is sprayed onto clover section again), it is packed into polyethylene plastic bag (25cm × 35cm), every bag of 300g is arranged 3 repetitions, uses sealing machine
It is evacuated and is sealed simultaneously, opened bag after the 40d that ferments at 30 DEG C, analyze alfalfa ensilage nutritional ingredient.
Comparative example 1
In addition to not adding leavening, other operating procedures are identical with embodiment 4, alfalfa ensilage prepared, as control
Group 1.
Preparing lactobacillus plantarum CICC20765 using the activation of embodiment 1, expansion culture and fermentation process, (purchase is in
State's Research for Industrial Microbial Germ preservation administrative center) fermentation liquid, the lactobacillus plantarum CICC20765 fermentation liquid crop that will be prepared
Alfalfa ensilage leavening, other operating procedures are identical with embodiment 4, prepare alfalfa ensilage, and as a control group 2.
Preparing bacillus coagulans ACCC10229 using the activation of embodiment 1, expansion culture and fermentation process, (purchase is certainly
Chinese agriculture Microbiological Culture Collection administrative center), by the bacillus coagulans ACCC10229 being prepared and embodiment 1
Lactobacillus plantarum CICC20765 fermentation liquid and according to 1: 1 mass ratio mixing after, as alfalfa ensilage leavening, other operations
Step is identical with embodiment 4, prepares alfalfa ensilage, and as a control group 3.
Using the activation of embodiment 1, expansion culture and fermentation process preparation Pediococcus acidilactici CGMCC1.4, (purchase is certainly Chinese
General Microbiological Culture preservation administrative center) fermentation liquid, by the Pediococcus acidilactici CGMCC1.4 fermentation liquid being prepared and implementation
After the lactobacillus plantarum CICC20765 of example 1 is according to 1: 1 mass ratio mixing, as alfalfa ensilage leavening, other operating procedures
It is identical with embodiment 4, alfalfa ensilage is prepared, as a control group 4.
Using the activation of embodiment 1, expansion culture and fermentation process preparation Pediococcus acidilactici CGMCC1.4, (purchase is certainly Chinese
General Microbiological Culture preservation administrative center) (purchase is from Chinese agriculture microorganism by fermentation liquid and bacillus coagulans ACCC10229
Culture presevation administrative center) fermentation liquid, by the bacillus coagulans ACCC10229 fermentation liquid being prepared, Pediococcus acidilactici
After CGMCC1.4 fermentation liquid and the lactobacillus plantarum CICC20765 fermentation liquid of embodiment 1 are mixed according to 1: 1: 1 mass ratio, make
For alfalfa ensilage leavening, other operating procedures are identical with embodiment 4, prepare alfalfa ensilage, and as a control group 5.
Preparing bacillus coagulans ACCC10229 using the activation of embodiment 1, expansion culture and fermentation process, (purchase is certainly
Chinese agriculture Microbiological Culture Collection administrative center) fermentation liquid, the bacillus coagulans ACCC10229 fermentation liquid that will be prepared
With the lactobacillus plantarum CICC20765 fermentation liquid of embodiment 1 and Pediococcus pentosaceus CICC22737 fermentation liquid according to 1: 1: 1 matter
After amount is than mixing, as alfalfa ensilage leavening, other operating procedures are identical with embodiment 4, prepare alfalfa ensilage, as
Control group 6.
Embodiment 5
To crude protein, dry matter and the thick rouge of the alfalfa ensilage that control group 1~6 in embodiment 2 and comparative example 1 is prepared
The content of fat is measured.Sample by the forage analysis China services center CVAS by near infrared spectrum (Near Infrared,
NIR it) is measured, specific measurement result is as shown in table 1.
Influence of the 1 different fermentations agent of table to nutrient composition content in alfalfa ensilage
As can be seen from Table 1, lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid are taken
With the content that can significantly increase crude protein in alfalfa ensilage, 5.4% is improved compared to 1~6 crude protein content of control group~
12.25%.Meanwhile dry matter content and crude fat content content are also higher, have successively been respectively increased 4.0% compared to control group
~9.75% and 0.97%~6.48%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of alfalfa ensilage leavening for improving crude protein content, which is characterized in that including lactobacillus plantarum CICC20765 and
Pediococcus pentosaceus CICC22737.
2. the preparation method of alfalfa ensilage leavening described in claim 1, which is characterized in that including by lactobacillus plantarum
CICC20765 and Pediococcus pentosaceus CICC22737 ferment respectively, will obtain lactobacillus plantarum CICC20765 fermentation liquid and
The mixing of Pediococcus pentosaceus CICC22737 fermentation liquid, obtains alfalfa ensilage leavening.
3. preparation method according to claim 2, which is characterized in that the lactobacillus plantarum CICC20765 fermentation liquid and
The mass ratio of Pediococcus pentosaceus CICC22737 fermentation liquid is 0.9~1.1: 0.9~1.1；The lactobacillus plantarum CICC20765
The viable count of fermentation liquid and Pediococcus pentosaceus CICC22737 fermentation liquid stands alone as 1.0 × 108~1.0 × 1010CFU/g。
4. preparation method according to claim 2, which is characterized in that the method for the fermentation includes: by lactobacillus plantarum
CICC20765 bacterium solution and Pediococcus pentosaceus CICC22737 bacterium solution are inoculated into respectively in liquid state fermentation culture medium, at 30~40 DEG C,
Ferment 20~28h under conditions of 80~120rpm, obtains lactobacillus plantarum CICC20765 fermentation liquid and Pediococcus pentosaceus
CICC22737 fermentation liquid；The liquid state fermentation culture medium takes water as a solvent, the component including following concentration: peptone 12g/L,
Powdered beef 12g/L, yeast powder 6g/L, glucose 24g/L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L, sodium acetate 5g/
L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L and tween 5mL/L, the pH value of the liquid state fermentation culture medium are 5.8~6.0.
5. the preparation method according to claim 4, which is characterized in that the work of the lactobacillus plantarum CICC20765 bacterium solution
Bacterium number is 1.0 × 108~1.0 × 1010CFU/g；The viable count of the Pediococcus pentosaceus CICC22737 bacterium solution is 1.0 × 108~
1.0×1010CFU/g。
6. the preparation method according to claim 4, which is characterized in that it is total that the inoculum concentration stands alone as liquid state fermentation culture medium
The 5%~15% of weight.
7. a kind of preparation method of alfalfa ensilage, includes the following steps:
1) lucerne that the alfalfa ensilage leavening or claim 2~6 any one method of clover and claim 1 are prepared
Mu silage fermentation agent mixing, obtains total mixture；
2) total mixture of the step 1) is sealed 40~50d of fermentation, obtains alfalfa ensilage.
8. preparation method according to claim 7, which is characterized in that clover and alfalfa ensilage leavening in the step 1)
Mass volume ratio be 1Kg: 1.4~1.6mL.
9. preparation method according to claim 7 or 8, which is characterized in that further include by lucerne before being mixed described in step 1)
Mu blueness leavening is diluted；The diluted multiple is 3~5 times.
10. preparation method according to claim 7, which is characterized in that the temperature being sealed by fermentation in the step 2) is 20
~30 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114854642A (en) * | 2022-05-30 | 2022-08-05 | 中国农业大学 | Alfalfa endogenous pediococcus pentosaceus EL5 and application thereof |
-
2019
- 2019-01-31 CN CN201910120736.5A patent/CN109820100A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 王丽学等: "5种乳酸菌对苜蓿青贮营养和发酵品质的影响", 《饲料研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114854642A (en) * | 2022-05-30 | 2022-08-05 | 中国农业大学 | Alfalfa endogenous pediococcus pentosaceus EL5 and application thereof |
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