CN109811063A - One kind SNP marker relevant to growth speed of pigs and its application - Google Patents

Abstract

The present invention provides one kind SNP marker relevant to growth speed of pigs and its application.The present invention is screened using sequencing technologies using TRPC1 gene as the candidate gene for influencing growth speed of pigs and identifies SNP site relevant to growth speed of pigs, and 2 SNP sites and genotype effects for influencing growth speed of pigs are obtained.2 SNP sites are located at the 595bp and 539bp of sequence shown in SEQ ID NO.1, its allelotype is C and T, and the allele C and C, T and T complete linkage in the genome of 2 SNP sites, therefore any of the above-described SNP site is all applied to the breeding of pig kind growth and development traits, and it is not limited by pig age, gender, kind, it can be used for the early stage breeding of pig, accelerate breeding process.

Description

One kind SNP marker relevant to growth speed of pigs and its application

Technical field

The present invention relates to molecular genetics fields, more particularly to SNP marker relevant to growth speed of pigs and are somebody's turn to do Application of the molecular labeling in breeding.

Background technique

Pork for a long time always occupy 60% or more of China's consumption of meat total amount, be the main body of Animal husbandry production. As the environmental consciousness of people gradually increases, Environmental Protection in China policy is gradually tightened, and the cost of pig breeding industry also gradually rises；Quickly The speed of growth corresponds to high efficiency, therefore the speed of growth is the important goal character in swine improvement work.And pig kind The speed of growth by a variety of transcription factors, epigenetic etc. complexity regulation, be always genetic breeding area research problem it One.Genotype, estimated breeding value are mainly inferred in traditional breeding using phenotype, and time-consuming, at high cost, and cannot select base Because of the individual of type homozygosis.Molecular Marker Assisted Selection Technology can individual to different development stage, organ even cell examine It surveys, directly reflects the sequence difference of DNA, while marker site is abundant, spread whole gene group, polymorphism is high, inheritance stability, no It is limited whether by environment and gene expression.It is the genetic improvement method economy, quick and effective, it is to your necessity of traditional breeding method Supplement.

The local pig breed in China is resourceful and with their own characteristics, provides precious resources for world's pig kind gene pool, also for The gene and molecular labeling for studying important economical trait provide good material.Hiding pig is the distinctive plateau type pig kind in China, is In the miniature pig strain that China is currently known body it is lightest one of, sow 0.4~0.6kg of Birth weight, fattening pig weight gain slowly, 12 monthly age weight are 20~25kg, 24 35~40kg of monthly age, 2~3 years 40~50kg of weight ability after life；But have simultaneously it is cold-resistant, The features such as resistance to crude feed, Fresh & Tender in Texture delicious, high-calcium low-fat is full of nutrition, green and healthy, conservation with higher and cultivation valence Value.Coal pig is the Ke Le pig in Guizhou, the Dahe Pig of Yunnan natural resources, the system of the Liangshan Mountain pig of the Zhaotong pig and Sichuan Province of Zhaotong City Claim.Coal pig figure is medium, adult weight boar weight 48.2kg, sow weight 69.5kg.The speed of growth is slow, stage of fattening daily gain 200g or so, lower than commercial varieties such as Ternary Pig.There is resistance to crude feed again simultaneously, high-quality stalwartness adapts to Altitude ecology ring The features such as border, fat deposition are high, lean meat percentage low delicious meat.The hiding pig and coal pig of the above difference speed of growth are research growths The good material of speed related gene.

With the continuous development of molecular quantitative and molecular biotechnology, have now been found that certain amount influences pig The functional gene and molecular labeling of the speed of growth, and apply in the breeding work of pig.As Zhang Hao et al. has found pig LXR α gene 5th site extron 20 1bp is that the individual growth speed of AA type is significantly higher than CC type individual (Zhang Hao, Zhang Bo, Xu Aishi wait Snp label relevant to growth speed of pigs and its application, CN 104404133A [P] .2015.)；Van Laere et al. is verified The growth of IGF2 gene regulation pig muscle, the G/A mutation at third exon 3 072 can influence commercial populations 10-20% Meat yield, wherein AA type be the speed of growth preponderant genotype (Van Laere A S, Nguyen M, Braunschweig M,et al.A regulatory mutation in IGF2causes a major QTL effect on muscle growth in the pig.[J].Nature,2003,425(6960):832-836.)；Fan et al. have found FTO gene with The intramuscular fat content and the speed of growth of pig are related.Wherein, haplotype 2 (- CTTGG-) individual growth of 5 SNP is fastest (Fan B,Du Z Q,Rothschild M F.The fat mass and obesity-associated(FTO)gene is associated with intramuscular fat content and growth rate in the pig.[J] .Animal Biotechnology,2009,20(2):58-70.)；Zhang et al. is it is again seen that with growth speed of pigs significant Relevant SNP marker, the CC type individual growth speed in the site C-1595A of the gene promoter area GHSR are significantly faster than CA type individual (Zhang B,Shang P,Tao Z,et al.Effect of a single nucleotide polymorphism in the growth hormone secretagogue receptor(GHSR)gene on growth rate in pigs[J] .Gene,2017,634.).But still lacks definite functions, effect at present significantly and may be directly applied to the molecular labeling in breeding.

TRPC1(transient receptor potential cation channel subfamily C member 1) memebrane protein of gene coding can form non-specific ion channel, by allowing positive ions especially calcium ion to pass through cell membrane And adjust the generation and function enforcement of muscle.People and mouse are more common in the research of TRPC1 gene at present, it is found that the gene is main The differentiation and migration for participating in regulation sarcoblast influence myotube development.

Summary of the invention

The purpose of the present invention is to provide SNP markers relevant to growth speed of pigs.

A second object of the present invention is to provide application of the SNP marker in the fast pig kind of the breeding speed of growth.

Applicant is in 3000 bull duroc group GWAS (whole-genome association, Genome-wide Association studies, GWAS) result in find that the muscle growth development of TRPC1 gene and pig is significant related.Pass through To pig (speed of growth is respectively hiding pig, the coal pig, Yorkshire Pigs from slow to fast) embryonic period, embryonic phase 60 of different building shape and the speed of growth The longissimus dorsi muscle of age in days carries out transcriptome analysis, as the result is shown expression quantity of the TRPC1 gene in Yorkshire and coal pig pole It is significantly higher than hiding pig, qPCR testing result is also consistent with this.Do not find TRPC1 gene polymorphic site and growth speed of pigs yet at present Association analysis report.Therefore, it is necessary to carry out the screening and identification of function SNP site to the gene, to directly apply to pig Selection and improvement.The present invention screens inhomogeneity using TRPC1 gene as the candidate gene for influencing growth speed of pigs, using sequencing technologies The SNP site of type between herds in swine difference identifies the correlation of SNP site and growth speed of pigs, obtains influence growth speed of pigs SNP site and genotype effects.

Present invention discover that SNP marker relevant to growth speed of pigs have 2, be located at TRPC1 gene (NC_ 010455.5) it originates at 5 ' flanking region codon upstream 1763bp, is denoted as C-1763T；Positioned at pig TRPC1 gene initiation codon At sub- upstream 1604bp, it is denoted as C-1604T.

In C-1763T, C is and the speed of growth relevant advantage allele fastly；In C-1604T, C is fast with the speed of growth Relevant advantage allele.

The complete linkage in the genome of both of the aforesaid SNP site need to only detect the genotype in the site C-1763T, so that it may To C-1604T loci gene type.

In SNP marker of the present invention, C-1763T is located at the 380th in nucleotide sequence shown in SEQ ID NO.1 Bit base；It can be obtained by nucleotide sequence primer pair amplifies as shown in SEQ ID NO.2-3；

C-1604T is located at the 539th bit base in nucleotide sequence shown in SEQ ID NO.1, can pass through nucleotides sequence The primer pair amplifies as shown in SEQ ID NO.2-3 are arranged to obtain.

The present invention provides a kind of for identifying the specific primer pair of the fast pig of the speed of growth, and nucleotide sequence is such as Shown in SEQ ID NO.2-3.

Kit containing above-mentioned specific primer pair belongs to the scope of protection of the present invention.

Specific primer pair the present invention provides above-mentioned SNP marker, for expanding SNP marker contains Following any application of the kit of the specific primer pair:

(1) application in the molecular marker assisted selection breeding of pig；

(2) application in the fast pig kind of the identification speed of growth；

(3) application in the SNP marker genotype is being detected.

The application is specifically includes the following steps: take pig genome dna to be tested, the primer shown in SEQ ID NO.2-3 PCR amplification is carried out, amplified production is sequenced, genotype of the detection sample to be tested pig in the SNP site；Genotype is CC Be the fast individual of the speed of growth, select genotype that can accelerate the genetic progress of speed of growth breeding for the individual of CC.

The concrete operations of one embodiment of the present of invention are as follows: genomic DNA is extracted from pig ear tissue, according to pig TRPC1 Gene 5' flanking region primers, it is as follows to obtain primer sequence: forward primer F:5'-CTAGAGTTGTGATGGGTCTTC- 3'(SEQ ID NO.2), reverse primer R:5'-GCTCATTGTAAATCTGTGGC-3'(SEQ ID NO.3).Draw shown in Object carries out PCR amplification in pig genomic DNA, and PCR product is sequenced after purification.

Sequencing file, and the nucleotide sequence ratio shown in sequence table SEQ ID NO.1 are opened using Chromas Pro software To analysis, C-1763T loci gene type is determined, if the site is the single peak C, be determined as CC genotype；If the site is The single peak T, then be determined as TT genotype；If the site is that C and T covers peak, it is determined as CT genotype.

The present invention also provides pig longissimus dorsi muscle TRPC1 genes to promote the application in muscle fast-growth.

Present invention demonstrates that the expression of the CC genotype promoter up-regulation pig TRPC1 gene of above-mentioned SNP marker.

The beneficial effects of the present invention are: SNP marker provided by the invention can carry out the speed of growth breeding of pig； And molecular labeling is not limited by the age of pig, gender, kind etc., can be used for the early stage breeding of pig, or even be just born in pig When can accurately be selected and remain, be substantially shorter the generation inteval, accelerate the Advances in Breeding of pig, improve the speed of growth of group. The present invention by detection SNP marker can early stage, quickly, it is accurate, low cost, effectively predict growth speed of pigs, in pig Kind improvement aspect has broad application prospects, and can obtain excellent economic value.

Detailed description of the invention

Fig. 1 is the mRNA expression of TRPC1 in pig longissimus dorsi muscle.TP represents hiding pig, and WJ represents coal pig, and LW represents big Yorker.

Fig. 2 is pig TRPC1 gene 5 ' flanking region different primers Gradient annealing.A-D respectively represents 1-4 to primer, Marker is DM2000plus, and annealing temperature is respectively 60,59.4,58.2,56,23.3,51.2,49.7,49 DEG C from left to right.

Fig. 3 is the sequencing peak figure in the site pig TRPC1 gene 5 ' flanking region C-1763T.

Fig. 4 is pig TRPC1 gene promoter luciferase activity.Control:pGL3-basic and pRL-TK cotransfection C2C12 cell；The promoter that CC and TT respectively indicates CC and TT homozygous individual is connected on PGL3-basic, with pRL-TK corotation Contaminate cell.

Fig. 5 is the site pig TRPC1 gene 5 ' flanking region C-1763T amplification；Agarose gel concentration is 2.5%；In figure Marker is DM2000plus；Each swimming lane clip size is 595bp in figure.

Fig. 6 is the site pig TRPC1 gene C -1763T individual sequencer map.

Specific embodiment

Following embodiment further illustrates the contents of the present invention, but should not be construed as limiting the invention.Without departing substantially from In the case where spirit of that invention and essence, to modifications or substitutions made by the method for the present invention, step or condition, the present invention is belonged to Range.

The expression analysis of TRPC1 gene in 1 pig longissimus dorsi muscle of embodiment

Selection place of china kind " hiding pig " (picking up from Tibet Agricultural and Animal Husbandry College's teaching practice pasture), " coal pig " (pick up from west Hiding Agriculture and Animal Husbandry College's teaching practice pasture) and introduced variety " Yorkshire Pigs " (pick up from Anhui section prosperous raise pigs breeding Co., Ltd) 6 Longissimus dorsi muscle tissue sample when the monthly age extracts tissue RNA using Trizol Traditional Method, and reverse transcription is at cDNA.

Pig TRPC1 gene mRNA sequence (accession number NM_001145751.1) is downloaded from NCBI, utilizes software Primer Premier 5.0 designs following primer:

Forward primer F:5'-CATCCAAAGGCAAGGTTA-3'

Reverse primer R:5'-AAGTCCGAAAGCCAAGTA-3'

CDNA with above-mentioned primer in hiding pig, coal pig and Yorkshire longissimus dorsi muscle tissue carries out real-time fluorescence quantitative PCR Amplification, PCR amplification generally use 20ul reaction system: 10 μ l2 × SuperReal PreMix Plus, 8 μ l ddH2O, up and down Swim each 1 μ l of 0.5 μ l, cDNA template of primer；By pcr amplification reaction system in 95 DEG C of initial denaturation 15min；Carry out following circulation 40 It is a: 95 DEG C of initial denaturations 20s, 58 DEG C of annealing 20s, 72 DEG C of extensions 20s, Melt 6s.With β-actin (upstream primer: 5'- GCCAACCGTGAGAAGATGACT-3'；Downstream primer: 5'-GTGACCCCATCCCCAGAGT-3') it is internal reference, utilize 2-^ΔΔCT Method carries out relative expression analysis (Fig. 1) of the TRPC1 gene in different cultivars pig longissimus dorsi muscle.

In figure in visible Yorkshire Pigs longissimus dorsi muscle tissue the significant plateau coal pig of TRPC1 gene expression amount and hiding pig.It should High expression of the gene in Yorkshire Pigs, illustrates that it may play important regulating and controlling effect to its muscle fast-growth.

The screening of 2 pig TRPC1 gene SNP site of embodiment

Selection place of china kind " hiding pig " (picking up from Tibet Agricultural and Animal Husbandry College's teaching practice pasture), " coal pig " (pick up from west Hiding Agriculture and Animal Husbandry College's teaching practice pasture) and introduced variety " Yorkshire Pigs " (pick up from Anhui section prosperous raise pigs breeding Co., Ltd) ear Tissue sample extracts ear tissue genomic DNA using phenol/chloroform method.

Pig TRPC1 gene order (accession number NC_010455.5) is downloaded from NCBI, utilizes software Primer Premier 5.0, to the DNA sequence dna for the TRPC1 gene downloaded from NCBI, four pairs of primers are devised, primer 1 expands pig TRPC1 gene 5' Control region -2142bp~-1548bp, purpose expanding fragment length are 595bp；Primer 2 expands pig TRPC1 gene 5' control region- 1702bp~-942bp, purpose expanding fragment length are 761bp；The amplification of primer 3 pig TRPC1 gene 5' control region -1121bp~- 662bp, purpose expanding fragment length are 550bp；Primer 4 expands pig TRPC1 gene 5' control region -678bp~+121bp,

Purpose expanding fragment length is 800bp.

Forward primer 1F:5'-CTAGAGTTGTGATGGGTCTTC-3'

Reverse primer 1R:5'-GCTCATTGTAAATCTGTGGC-3'

Forward primer 2F:5'-TTACCCCTAAACACTCTGG-3'

Reverse primer 2R:5'-CATTGGGATCGGCTCTA-3'

Forward primer 3F:5'-CAAGCACTAAGGGGACA-3'

Reverse primer 3R:5'-GGTTGCAGGTTTGAGGTA-3'

Forward primer 4F:5'-ACCTCAAACCTGCAACC-3'

Reverse primer 4R:5'-ACCTCCCGCACATCCTT-3'

PCR amplification is carried out in hiding pig, coal pig and Yorkshire genomic DNA with above-mentioned primer, PCR amplification is anti-using 20ul Answer system: 10 μ l 2 × Power Taq PCR Master Mix, 8 μ lddH2O, upstream and downstream primer each 0.5 μ l, 1 μ of DNA profiling l；By pcr amplification reaction system in 94-95 DEG C of initial denaturation 5min；Carry out 35: 94-95 DEG C initial denaturation 30s of circulation as follows, gradient Anneal 30s, 72 DEG C of extension 40s；72 DEG C of extension 7min determine the optimum annealing temperature (figure that is 58 DEG C to get arriving PCR product 2)。

(Shanghai biotechnology is purchased from through Gel Extraction Kit kit to the PCR product of above-mentioned 3 pig kinds Co., Ltd operates according to kit specification) purifying.Each pig kind selects 10 individuals to carry out PCR amplification, each individual respectively PCR product respectively take 5 μ l, (Hua Da gene) is sequenced at a sample in mixed pond.

The mixed pond sequencing result of three pig kinds through Chromas Pro software compare analyze, pig TRPC1 upstream region of gene about 7 SNP sites are detected in the region of 2000bp, according to its distance and allele in the gene start codon upstream Type is denoted as A-1825T, C-1763T, C-1604T, A-1166G, C-985A, A-730G and G-227T respectively.According to sequencing peak figure Pu'an section, wherein C-1763T (Fig. 3) and C-1604T may genotype frequency differs greatly between kind site.Two positions Point distance 123bp, and allelotype -1763C and -1604C complete linkage, form CC haplotype；- 1763T and -1604T is complete It is complete chain, form TT haplotype, it may be possible to regulate and control the important site of the gene differential expression.Therefore the application can only detect C- 1763T loci gene type can represent the haplotype combination of 2 sites (C-1763T, C-1604T).

3 pig TRPC1 gene SNP site section promoter regulation Activity determination of embodiment

According to pig TRPC1 gene 5' Regulatory Sequence design primer, primer sequence are as follows:

Forward primer F:5'-CTAGAGTTGTGATGGGTCTTC-3'；

Reverse primer R:5'-GCTCATTGTAAATCTGTGGC-3'

The primer amplification pig TRPC1 gene 5' control region -2142bp~-1548bp, wherein including C-1763T and C- Two SNP sites of 1604T, purpose expanding fragment length are 595bp, and nucleotide sequence is as shown in SEQ ID NO.1.

Using the pig genomic DNA of different genotype as template, is expanded using PCR reaction, obtain TRPC1 gene 5' tune The control area site C-1763T and C-1604T is two kinds of haplotype sequences of C/C and T/T, is denoted as CC and TT respectively.To the segment of amplification It carries out digestion, purify and connect and compose recombinant pGL3-basic-TRPC1, will be recombinated by the method for lipo2000 liposome Carrier is transfected into C2C12 cell, using renilla luciferase carrier pRL-TK as internal reference, is carried out with luciferase reporter gene Luciferase assays, testing result are as shown in Figure 4.

The luciferase expression of the sequence fragment of visible CC type will be significantly higher than TT type in figure, illustrate when on TRPC1 gene When the trip site 1763bp and 1604bp is C allelotype, promoter fragment regulation activity with higher in vivo may be used Can have the function of raising pig TRPC1 gene expression.

4 pig TRPC1 gene C -1763T loci gene type of embodiment detects sequencing approach and establishes to quickly and easily detect Pig TRPC1 gene C -1763T loci gene type, using pig TRPC1 gene order (accession number NC_010455.5) design primer, Primer sequence is as shown in SEQ ID NO.2 in sequence table and SEQ ID NO.3.PCR amplification, PCR are carried out to the genomic DNA of pig Reaction system is 20ul system: 10 μ l 2 × Power Taq PCR Master Mix, 8 μ l ddH2O, upstream and downstream primer each 0.5 μ l, 1 μ l of DNA profiling；By pcr amplification reaction system in 94-95 DEG C of initial denaturation 5min；It is pre- to carry out following circulation 35: 94-95 DEG C It is denaturalized 30s, 58 DEG C of annealing 30s, 72 DEG C of extension 40s；72 DEG C of extension 7min, that is, obtain PCR product.

The agarose gel electrophoresis that pcr amplification product concentration is 2.5%, ethidium bromide staining, in gel imaging system Observe PCR amplification effect, it is seen that clip size is the clear band of 595bp (see Fig. 5).PCR product is sequenced.Using Chromas Pro software opens sequencing file, and the nucleotide sequence comparison shown in sequence table SEQ ID NO.1 is analyzed, and determines C-1763T loci gene type is determined as CC genotype if the site is the single peak C；If the site is the single peak T, It is determined as TT genotype；If the site is that C and T covers peak, it is determined as CT genotype (Fig. 6).

Genotype of the genetic marker that 5 present invention of embodiment screens in different swinerys is distributed detection

Acquisition hiding pig (picking up from Tibet Agricultural and Animal Husbandry College's teaching practice pasture), coal pig (it is real to pick up from Tibet Agricultural and Animal Husbandry College's teaching Practise pasture), Yorkshire Pigs (prosperous pig raising breeding Co., Ltd, Anhui section) ear tissue sample, it is a that pig is extracted using phenol/chloroform method Body genomic DNA.

The PCR product sequencing technologies established using embodiment 4, measure 3 sites kind individual TRPC1 gene C -1763T Genotype, testing result is as shown in table 1.

The distribution of 1 pig TRPC1 gene C -1763T loci gene type of table

Hiding pig, coal pig belong to the slow place of china pig variety of the speed of growth, and the speed of growth and weight, which are below, introduces pig Kind Yorkshire Pigs, wherein the speed of growth Yu weight of coal pig slightly above hide pig.The pig kind of visible growth fast speed in table 1 The C gene frequency of (Yorkshire Pigs) in the site is 1, and in the C equipotential base of the slow grower pigs kind (coal pig) in China place Because frequency is 0.621, the C gene frequency of the slower China place miniature pig kind of another speed of growth (hiding pig) is 0.441.I.e. the frequency of C allele is positively correlated in the speed of growth of pig and group.Thus tentatively judge TRPC1 gene C- The site 1763T CC type is to grow fireballing genotype, and TT type is to grow slow-footed genotype.The result shows that the gene loci Genotype and allele distributions difference between the swinery of different growth performances it is obvious, may be related to growth performance.

TRPC1 genotype and growth speed of pigs correlation analysis in 6 Huai Pig new line group of embodiment

It acquires 102 Huai Pig new lines and (prosperous pig raising breeding Co., Ltd, Anhui section is picked up from, for the 4th generation of the new lines Group) ear tissue sample, individual between affinity-less relation, it is all individual measurement and calculate up to 30kg weight age in days and reach 90kg body 2 growth and development traits indexs (being shown in Table 2) of age in days of weight.

2 Huai Pig new line growth and development traits index of table

The base in the site TRPC1 gene C -1763T of 102 Huai Pig new lines is measured using the technology established in embodiment 4 Because of type, it the results are shown in Table 3.CC type frequency in 3 kinds of genotype that the site TRPC1 gene C -1763T occurs in Huai Pig new line group 78.43%, CT type frequency 20.59%, TT type frequency is extremely low, and only 0.98%.

The distribution of table 4 generation of 3 Huai Pig new line TRPC1 gene C -1763T loci gene type

TRPC1 gene C -1763T loci gene type in Huai Pig new line group and growth and development traits are associated point Analysis, the results are shown in Table 4.The age in days that CC type pig reaches 30kg and the age in days for reaching 90kg are said significantly less than CT type pig (P < 0.05) The early stage (before 30kg weight) of bright CC type pig and the speed of growth in growth period (30kg-90kg) are above CT type pig.CC type pig ratio CT type pig early reaches 30kg weight for 8.59 days, early to reach 90kg weight within 20.04 days, it is seen that allele C is point of fast-growth Son label, can be used for pig molecule mark assistant breeding.

The site table 4 generation of 4 Huai Pig new line TRPC1 gene C -1763T different genotype is to growth performance

Effect analysis

Reach age in days	CC (n=80)	CT (n=21)	TT (n=1)
				30kg	92.45±0.97a	101.04±2.49b	128.66
90kg	189.27±4.30a	209.31±6.85b	209.93

Note: CC, CT, TT represent three kinds of genotype, and n represents statistics number, indicated between different letters significant difference (P < 0.05), numerical value is average ± standard error in table.TT genotype due to only one individual, not with remaining two kinds of genotype into Row significance analysis.

Although having used general explanation, specific embodiment and test above, the present invention is described in detail, But on the basis of the present invention, it can be made it is some modify or improve, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Sequence table

<110>China Agricultural University

<120>a kind of SNP marker relevant to growth speed of pigs and its application

<130> KHP191110613.3

<160> 13

<170> SIPOSequenceListing 1.0

<210> 1

<211> 595

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 1

ctagagttgt gatgggtctt ctctctagta ccggaggttg agtgagtagc tttaagtaat 60

gtgctggttc tgaatcttac atgctattgt tcatcatgac cttatcattg gtctcctatc 120

atagatgcaa atagaggatc ttaaaatatc ccagtttatg ctggataaaa atggacgaga 180

gaagcaagag agaatattct gcatctgagt tgattcaaaa agaaatctac aacattagct 240

tactgtgaag gaggaaaaac cctctaataa ttttggaaag tctgctagga ttatctccta 300

aattagactg gaattacact gaatctaaga aatcaatcca gcccctaagg gagttctatg 360

gataaggagt cacctaatcc tagaagctga aataaacttc atattaatgt ggtttctata 420

gttcagagcc atctgaattt ttacccctaa acactctgga acttttggat ggatcagggt 480

tagagttctg ccactgagta aaaattttac tggactccaa aatttgtaag cacacacaca 540

taaacaaacc caaatatgta ccactgtgtt cactggccac agatttacaa tgagc 595

<210> 2

<211> 21

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 2

ctagagttgt gatgggtctt c 21

<210> 3

<211> 20

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 3

gctcattgta aatctgtggc 20

<210> 4

<211> 18

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 4

catccaaagg caaggtta 18

<210> 5

<211> 18

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 5

aagtccgaaa gccaagta 18

<210> 6

<211> 21

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 6

gccaaccgtg agaagatgac t 21

<210> 7

<211> 19

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 7

gtgaccccat ccccagagt 19

<210> 8

<211> 19

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 8

ttacccctaa acactctgg 19

<210> 9

<211> 17

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 9

cattgggatc ggctcta 17

<210> 10

<211> 17

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 10

caagcactaa ggggaca 17

<210> 11

<211> 18

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 11

ggttgcaggt ttgaggta 18

<210> 12

<211> 17

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 12

acctcaaacc tgcaacc 17

<210> 13

<211> 17

<212> DNA

<213>artificial sequence (Artificial Sequence)

<400> 13

acctcccgca catcctt 17

Claims (7)

1. a kind of SNP marker relevant to growth speed of pigs, which is characterized in that the SNP marker is located at TRPC1 gene It originates at 5 ' flanking region codon upstream 1763bp, is denoted as C-1763T；Or it is located at pig TRPC1 gene start codon upstream At 1604bp, it is denoted as C-1604T.

2. SNP marker according to claim 1, which is characterized in that in C-1763T, C is related fastly to the speed of growth Advantage allele；In C-1604T, C is and the speed of growth relevant advantage allele fastly.

3. SNP marker according to claim 1 or 2, which is characterized in that in the SNP marker, C-1763T 380th bit base in the nucleotide sequence shown in the SEQ ID NO.1；It can pass through nucleotide sequence such as SEQ ID NO.2- Primer pair amplifies shown in 3 obtain；

C-1604T is located at the 539th bit base in nucleotide sequence shown in SEQ ID NO.1, can be by nucleotide sequence such as Primer pair amplifies shown in SEQ ID NO.2-3 obtain.

4. a kind of for identifying the specific primer pair of the fast pig of the speed of growth, which is characterized in that its nucleotide sequence such as SEQ Shown in ID NO.2-3.

5. the kit containing specific primer pair described in claim 4.

6. claim 1-3 any SNP marker, specific primer pair as claimed in claim 4 or the claim 5 Following any application of the kit:

(1) application in the molecular marker assisted selection breeding of pig；

(2) application in the fast pig kind of the identification speed of growth；

(3) application in the SNP marker genotype is being detected.

7. application as claimed in claim 6, which comprises the following steps:

Pig genome dna to be tested is taken, answers primer shown in SEQ ID NO.2-3 to carry out PCR amplification, amplified production is surveyed Sequence, genotype of the detection sample to be tested pig in the SNP site；C is advantage allele relevant to fast-growth, for giving birth to The breeding of long fireballing pig kind.