CN109806320A - A kind of Fu tea extraction containing coronoid process dissipate capsule bacterium living, preparation method and applications - Google Patents
A kind of Fu tea extraction containing coronoid process dissipate capsule bacterium living, preparation method and applications Download PDFInfo
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Abstract
The present invention provides a kind of preparation methods of Fu tea extraction containing coronoid process dissipate capsule bacterium living: using Fu tea as raw material, low temperature drying sieving or wet concentration sieving separate coronoid process dissipate capsule bacterium and tealeaves；Tealeaves extracts after adding water or refluxing extraction, obtains tealeaves primary extract；Coronoid process dissipate capsule bacterium is added in tealeaves primary extract, is mixed, the Fu tea extraction containing coronoid process dissipate capsule bacterium living is obtained.Extract is added to tablet, noodles, solid beverage, in rice flour production process, obtains the product of the Fu tea extraction containing coronoid process dissipate capsule bacterium living.Fu tea extraction of the invention and Related product, both it ensure that recovery rate, long-time high temperature is in turn avoided to extract to the active destruction of coronoid process dissipate capsule bacterium, coronoid process dissipate capsule bacterium is set to play probiotic action, it is horizontal with more TC, TG and LDL-C is reduced, blood viscosity is reduced, the effect of strengthen immunity can be used for preparing drug, health food and functional food.The present invention provides foundation for utilization and extention and deep processing Fu tea, and provides new application direction.
Description
Technical field
The invention belongs to medicine food technical fields, and in particular to a kind of to contain coronoid process dissipate capsule bacterium living by raw material preparation of Fu tea
Fu tea extraction and Related product method, and its preparation adjust blood fat and improving blood drug, health food and function
Application in the products such as food.
Background technique
Fu tea is, by the post-fermented tea of reprocessing, it is big basic to belong to China six using Dark Green Tea or thick old green tea as raw material
One kind of dark green tea and full fermentation tea in teas.Fu tea is with a long history, because its be in hot summer days summer processing and fabricating, fragrance and
Effect claims Fu tea again similar to Poria cocos.The principal mode of Fu tea is compressed tea, i.e., in the production process for the ease of transporting and protect
Hiding, the producer are pressed the pattern for being processed into brick, seem its shape as brick, it is also known as " Fu-brick teas ".Currently, Fu tea
There is the sale of the forms such as loose tea, tea cake.Fu tea is mainly sold in border areas such as Xinjiang, Gansu, Tibet etc., be commonly called as " brick tea " or
" border-sale tea " is exported to more than 40 countries such as the Central Asia, West Asia along " Silk Road ", is known as " mystery on the silk road
Tea ", " the black gold on the Silk Road ".
Modern pharmacological studies have shown that Fu tea has significant rush digestion, reducing blood lipid, nourishing the stomach weight reducing, adjusts glycometabolism, inhibits
The health efficacies such as tumour cell and strengthen immunity.Long-term drinking Fu tea, can promote human metabolism, improve immunity,
It builds up health, delay senescence, there is apparent medicine healthy sofa and pathology prevention effect.
Studies have shown that the pharmacological activity of Fu tea is a kind of excellent of self-assembling formation in tealeaves active constituent itself and fermentation process
Gesture bacterium-coronoid process dissipate capsule bacterium (Eurotium cristatum) coefficient result.Quality and medicine of the coronoid process dissipate capsule bacterium to Fu tea
The performance of reason effect has played important function.Coronoid process dissipate capsule bacterium is referred to as " golden flower bacterium " in Fu tea, belongs to Eurotiale Trichocomaceae and dissipates
A kind of celadon Aspergillus fungal of capsule Pseudomonas is suitble to grow in soil, Fu-brick tea, cordyceps sinensis, Chinese medicine tablet and sawdust.Coronoid process
Bulk bacteria are made of ascocarp and mycelia.Coronoid process dissipate capsule bacterium can produce active skull cap components abundant, to impart Fu-brick tea
Distinctive color and Fu tea promote digestion, lower blood-fat and reduce weight, inhibit the health-care efficacies such as tumour cell and treatment cardiovascular disease
Material base.The main matter for the performance drug effect that coronoid process dissipate capsule bacterium generates is exocellular polysaccharide, Lovastatin constituents, indoles life
(Liu Liping, Tang Yuwei, Wang Ruoxian, Li Zhibing, Liu Zhonghua, Liu Shuoqian, Fu tea the coronoid process dissipate capsule bacterium hair such as alkaloids, uranidin substance
Ferment produces the research of indole alkaloid, tea science 2017,37 (5): 503-512).Lu Hengqian (Agricultural University Of Anhui master opinion
Text, coronoid process dissipate capsule bacterium liquid state fermentation prepare instant dark tea and its functional study, tutor Yue Pengxiang) it reports and utilizes liquid fermentation system
The technique of standby instant dark tea.Yuan Yong (Agricultural University Of Hunan's master thesis, Fu tea " golden flower " fungus extract pharmacological effect and change
The Primary Study studied point, tutor Liu Zhonghua) report extraction Separation Research to coronoid process dissipate capsule bacterium conidia powder.Yi Fengying etc.
(Yi Fengying, Liu Suchun, Yuan Xiao, Zhao Le, the research of coronoid process dissipate capsule bacterium ascospore wall-breaking method, food and machinery 2011,27
(3): 137-139. the wall-breaking method of coronoid process dissipate capsule bacterium ascospore) is reported.(the Advance Journal of Food such as Lu
Science and Technology 2016,10 (8): 591-596.) report coronoid process dissipate capsule bacterium fermentation dark green tea in it is effective at
Divide research.Zou etc. (Molecules 2014,19,17839-17847) reports the alkaloids that coronoid process dissipate capsule bacterium fermentation generates
Compound.Fu etc. (Food Research International 2011,44:2999-3005.) reports the reducing blood lipid of Fu tea
Activity mechanism.Yan etc. (Food and Agricultural Immunology 2017,28 (3): 388-402.) reports Fu
The effect and mechanism that coronoid process dissipate capsule bacterium activation in tea is immunized.
Currently, the deep processed product of Fu tea beats powder with tealeaves, be added in relevant food based on, this kind of product processing essence
Degree not enough, causes product uniformity coefficient, mouthfeel bad, it is difficult to promote.Fu tea is extracted, is prepared into extract, and then make
At or be added Related product in deep processing technique then only have several report.201410056293.5 invention of Chinese patent ZL
Fu tea is directly extracted with water high temperature, is prepared as chewing after spray drying obtains extract by a kind of Fu tea extraction chewable tablets
Piece.Chinese patent application 201010278854.8 discloses the method that tea polyphenols are prepared from Fu tea.Chinese patent application
201710193496.2 disclosing the compound preparation comprising Fu tea extract.Chinese patent application 201210570124.4,
201710926203.7 disclose the dry method for being prepared as Related product of extraction of the compound tea containing Fu tea.It is above this straight
Connecing obviously has biggish loss to the activity of coronoid process dissipate capsule bacterium with the technique that water high temperature extracts.Chinese patent application
201610680157.2 disclosing Fu tea organic solvent or CO2It extracts, the extract with effect for reducing blood fat is made.
It is known that organic solvent has the ability for destroying bacterium normal physiological structure, this extraction process also would detract from the work in Fu tea
Property coronoid process dissipate capsule bacterium.
Have no related the disclosure as set forth herein using Fu tea as raw material, Fu tea extraction of the preparation containing coronoid process dissipate capsule bacterium living and
The research of the preparation process of Related product or report.The resulting extract of the present invention, had not only been able to maintain the vigor of coronoid process dissipate capsule bacterium, but also
It can guarantee the recovery rate of the effective components such as Tea Polyphenols in Tea.
Summary of the invention
The object of the present invention is to provide one kind using Fu tea as raw material, Fu tea extraction of the preparation containing coronoid process dissipate capsule bacterium living
Method is realized by following steps: using the Fu tea for completing fermentation as raw material, being sieved through low temperature drying sieving or wet concentration, by coronoid process
Bulk bacteria (golden flower) are separated with tealeaves；The 8-12 times of water measured, room is added by tealeaves/water (weight/volume) in resulting tealeaves
Temperature extraction or refluxing extraction 2-3 time, combined extract, be concentrated under reduced pressure, dry, obtain tealeaves primary extract；By isolated hat
Prominent bulk bacteria (golden flower) is added in tealeaves primary extract, is mixed to get the Fu tea extraction containing coronoid process dissipate capsule bacterium living.
The above-mentioned preferred loose tea of Fu tea raw material need to first disperse tea cake or brick tea if raw material form is tea cake or brick tea；Wherein
Preferred Aqua Folium Camelliae sinensis Extraction technique is the water of 12 times of amounts, refluxing extraction 2 times, every time 2 hours；During wherein Aqua Folium Camelliae sinensis extracts
Drying means include but is not limited to dry, spray drying and freeze-drying.
The method of low temperature drying sieving in preparation process are as follows: the Fu tea for taking dispersion is dried at lower than 60 DEG C, rubbed by hand
Stranding or mechanical stirring, fall coronoid process dissipate capsule bacterium from tealeaves surface, are sieved through sieve multistage, obtain coronoid process dissipate capsule bacterium thallus；System
The method of wet concentration sieving in standby technique are as follows: take Fu tea to be added the 8-12 times of water measured, soaking at room temperature is simultaneously rubbed or machinery stirs by hand
It mixes, falls on coronoid process dissipate capsule bacterium in water from tealeaves surface, be sieved through sieve multistage, obtain aqueous coronoid process dissipate capsule bacterium thallus, it is low
Temperature is obtained by drying.The screen sizes of above-mentioned sieving are 50-300 mesh, carry out multistage sieving, preferably 80 mesh, 120 mesh, 200 mesh three
Grade sieving.
It is above-mentioned to rub preferably soft, the churned mechanically suitable low speed of speed by hand, it is no more than 800rpm, such as 200rpm, 400rpm
Or 600rpm etc. generates excessive tealeaves fine powder to prevent tealeaves from excessively being smashed.
The above-mentioned Fu tea extraction containing coronoid process dissipate capsule bacterium living, in preparation process, when being concentrated under reduced pressure and drying process
When technological temperature is lower than 60 DEG C, coronoid process dissipate capsule bacterium (golden flower) can also be added in advance in concentration or drying process technique.
The preparation method of the above-mentioned Fu tea extraction containing coronoid process dissipate capsule bacterium living, the method are also applied for other and contain coronoid process
The preparation of the extract containing viable bacteria of the fermentation teas of bulk bacteria.
It is a further object of the present invention to provide the methods that this Fu tea extraction is prepared as Related product, appropriate by adding
Supplementary material, various oral Pharmaceutical dosage forms, including but not limited to tablet, capsule, granule can be made；Various foods can be made
Product dosage form, including but not limited to pressed candy, solid beverage, noodles, flour, rice flour.
Added supplementary material includes that can be used for various rules and regulations is medicinal in said medicine dosage form manufacturing process
Auxiliary material；Above-mentioned food formulation includes food processing auxiliary material, the food additives that can be used of various rules and regulations etc..
Another object of the present invention is to provide the immune function of reducing blood lipid, protection blood vessel and enhancing of prepared food and drug
Energy.Show coronoid process dissipate capsule bacterium can be made to play containing the Fu tea extraction and Related product of coronoid process dissipate capsule bacterium living through pharmacodynamic study
The effect of probiotics and the health-care effect for playing tea extract active material, having reduces serum total cholesterol (TC), glycerol
Three esters (TG) and low-density lipoprotein (LDL-C) are horizontal, reduce blood viscosity, show that it has and adjust blood lipid and improve blood
Effect can be used for preparing the products such as drug, health food and the functional food for adjusting blood lipid and improvement blood.
With known document and Patent Reference, the present invention is than Chinese patent ZL 201410056293.5, patent application
201010278854.8, patent application 201710193496.2, patent application 201210570124.4, patent application
Water high temperature described in 201710926203.7 extracts the activity for being more able to maintain coronoid process dissipate capsule bacterium and guarantees mentioning for tealeaves active material
Take rate.It is directly extracted for a long time with water high temperature, has greater loss to the activity of coronoid process dissipate capsule bacterium, if water temperature and extraction time are not
Foot is then difficult completely to extract the effective components such as tea polyphenols in tealeaves.The present invention compares Chinese patent application
201610680157.2 the organic solvent or CO2It extracts, is more able to maintain the activity of coronoid process dissipate capsule bacterium, and production cost
It is low, operation it is easier, the aqueous soluble active constituent extracted is more comprehensive.
Fu tea extraction step of preparation process provided by the invention containing coronoid process dissipate capsule bacterium living is simple and easy, practicability
By force, low in cost, the recovery rate of the effective components such as vigor and the Tea Polyphenols in Tea of coronoid process dissipate capsule bacterium can be kept simultaneously.
The present invention develops a kind of extraction using Fu tea as raw material, with adjusting blood lipid, improvement blood and strengthen immunity
The purposes of object and its Related product provides foundation for utilization and extention and deep processing Fu tea, and provides new application direction.
Detailed description of the invention
Fig. 1 be in embodiment 4 directly organic solvent (ethyl alcohol) extract (A), remove the water extract (B) of golden flower and direct
Water mentions the thin-layer chromatogram (solvent: chloroform: methanol: ammonium hydroxide=25:3:1) of extract (C), and coloration method is respectively 254nm
It is ultraviolet, 365nm is ultraviolet, iodine vapor colour developing.
Specific embodiment
The present invention is further described with reference to the drawings and specific embodiments.
Embodiment 1
Fu tea loose tea 5kg is taken, in 60 DEG C of stirring and dryings, respectively with 50 mesh and 200 mesh sieves, isolated coronoid process dissipates capsule
The water of 12 times of amounts is added by tealeaves/water (weight/volume), refluxing extraction 2 times, 2 hours every time, is closed for bacterium 45g, remaining tealeaves
And extracting solution, it is concentrated under reduced pressure, 100 DEG C of decompression dryings crush, and obtain tealeaves primary extract 1253g；Isolated coronoid process is dissipated into capsule
Bacterium (golden flower) is added in tealeaves primary extract, mixes to get the Fu tea extraction 1298g containing coronoid process dissipate capsule bacterium living, total to extract
Rate is 26.0%.
Embodiment 2
Fu tea loose tea 10kg is taken, the water of 8 times of amounts is added by Fu tea/water (weight/volume), mechanical stirring 6 is small at room temperature
When, 50 mesh of gained liquid, 80 mesh, 120 mesh, the filtering of 200 mesh level Four take 200 mesh filter cakes, and it is dry, obtain golden flower bacterium 58g.Filter
Liquid continuously adds in tea grounds, and refluxing extraction 2 times, 1 hour for the first time, second 2 hours, combined extract will be isolated
Coronoid process dissipate capsule bacterium (golden flower) be added in extracting solution, be freeze-dried, obtain freeze-drying powder extracts, i.e., containing coronoid process dissipate capsule bacterium living
Fu tea extraction 1886g, total recovery rate be 18.9%.
Embodiment 3
Fu brick tea tea 5kg is taken, is fritter by brick tea hand breaking, rubs broken, mechanical dispersion, in 50 DEG C of stirring and dryings, respectively
With 120 mesh and 250 mesh sieves, isolated coronoid process dissipate capsule bacterium 38g, remaining tealeaves is added by tealeaves/water (weight/volume)
Enter the water of 10 times of amounts, room temperature refluxing extraction 2 times, 3 hours every time, combined extract was concentrated under reduced pressure, and decompression drying obtains tealeaves
Primary extract；Isolated coronoid process dissipate capsule bacterium (golden flower) is added in tealeaves primary extract, mixes and dissipates capsule to get containing coronoid process living
The Fu tea extraction 889g of bacterium, total recovery rate are 17.8%.
4 comparative experiments of embodiment
Fu brick tea tea 50g is taken to rub by hand scattered, low temperature drying stirring, continuous 80 mesh of mistake, 120 meshes obtain golden flower 0.537g.
By tealeaves/water (weight/volume), remaining tea leaves take 5g to be extracted one time 2 hours with 12 times of amount water and are evaporated, and obtain extract
0.893g；By tealeaves/water (weight/volume), adds second of 12 times of amount water extractions and be evaporated for 2 hours, obtain extract
0.531g；
By tealeaves/water (weight/volume), takes the direct 12 times of amount water of Fu tea 5g to extract one time 2 hours and be evaporated, extracted
Object 1.086g；
By tealeaves/ethyl alcohol (weight/volume), takes the direct 12 times of amount ethyl alcohol of Fu tea 5g to extract one time 2 hours and be evaporated, obtain
Direct ethanol extract 2.03g；Three kinds of extracts extracted for the first time are evaporated, and are uniformly settled to 50mL with methanol, quantitative contact plate,
With chloroform: methanol: ammonium hydroxide=25:3:1 solvent expansion is to get attached drawing 1.
The recovery rate of organic solvent (ethyl alcohol) extract is big it can be seen from result above and attached drawing 1, contained by extract
Substance differs larger with two kinds of water extracts.The recovery rate of two kinds of water extracts is not much different, and ingredient contained by extract also differs not
Greatly, R in the extract of golden flower bacterium is removedf=0.5 or so component content is higher than direct water extract.
The preparation of 5 Fu tea extraction tablet of embodiment
2 gained Fu tea extraction 20.0g of Example and starch 100g is mixed, and adding mass fraction is 10% starch slurry 2g system
At softwood, be added magnesium stearate 0.1g, dried starch 8g be pressed into after mixing 400, the tablet of 0.3g/ piece to get.Every contains Fu
Tea extraction 50mg.
The preparation of 6 Fu tea extraction solid beverage of embodiment
2 gained Fu tea extraction 20.0g of Example and maltodextrin 500g is mixed, and xylitol 20g is added, and is mixed, is added
Suitable quantity of water granulation, it is dry, it is packed as every bag of 2g, amounts to 360 bags to obtain the final product.Every bag of 55mg of tea extraction containing Fu.
The preparation of 7 Fu tea extraction noodles of embodiment
Flour 450g, buckwheat 20g are taken, 3 gained Fu tea extraction 10g of embodiment adds suitable quantity of water, edible salt, kneads shape
At dough, place 30 minutes or more.By above-mentioned dough compression moulding, cut, it is dry, then cut, it is packaged as the every bundle of 30g, totally 16
Bundle.Every bundle tea extraction containing Fu 625mg.
The preparation of 8 Fu tea extraction rice stick of embodiment
Powder material 450g is taken, 3 gained Fu tea extraction 15g of embodiment is to get the powder material containing Fu tea extraction.
Further plus suitable quantity of water, edible salt, stirring are pressed into vermicelli type, cut, dry, then cut, and are packaged as the every bundle of 15g, totally 31 bundle.
Every bundle rice stick tea extraction containing Fu 484mg.
Coronoid process dissipate capsule bacterium contains quantifier elimination in 9 product of embodiment
9.1 materials and methods
Material and instrument: the Fu tea extraction of embodiment 3, the tablet of embodiment 5, the solid beverage of embodiment 6, embodiment
The rice stick of 7 noodles, embodiment 8；PDA culture medium (Beijing overpass technical concern Co., Ltd)；Coronoid process dissipate capsule bacterium standard bacteria
Strain (China General Microbiological culture presevation administrative center, deposit number are CGMCC No.10610).The training of II mould of MJ-160BSH-
It supports case (Shanghai new talent medical instrument Manufacturing Co., Ltd)；AESAP1064 slap type homogenizer (Mei Liai diagnostic products (Shanghai)
Co., Ltd)；II A2 Biohazard Safety Equipment (SuZhou Antai Air Tech Co., Ltd.) of BHC-1300；UPH-3-20T water purification machine (four
The excellent general ultrapure Science and Technology Ltd. in river)；QL-901 vortex oscillator (its woods Bell's instrument manufacturing Co., Ltd)；CX41 microscope
(Olympus (China)Co., Ltd.)；The omnipotent fluorescence digit microscope of EVOSf1 (AMG Co., the U.S.).
Experimental method: referring to GB/T 9833.3-2013 " compressed tea Part III: Fu-brick tea " method require and
GB4789.15-2016 national food safety standard food microbiological examination mould and yeast counts method are measured.
9.2 measurement result
The testing result of 1 coronoid process dissipate capsule bacterium content of table
| Title | Specification | Coronoid process dissipate capsule bacterium content (× 104CFU/g) |
| Fu-brick tea | 400g/ block | 49±3 |
| Fu tea extraction | - | 225±16 |
| Tablet | 0.3g/ piece | 31±5 |
| Solid beverage | 2g/ bags | 7.5±1 |
| Noodles | The every bundle of 30g | 4.5±0.8 |
| Rice stick | The every bundle of 15g | 6.8±0.4 |
The above result shows that Fu tea is extracted as the Fu tea extraction containing coronoid process dissipate capsule bacterium according to the method for the present invention, it can
To greatly improve the quantity of the active bacteria in material, be conducive to for Fu tea to be prepared into highly concentrated health-oriented products.To extract
Object is added in various products, relative to Fu tea powder is directly added into, can also greatly improve containing for coronoid process dissipate capsule bacterium in the product
Amount increases value-added content of product.
Embodiment 10 adjusts blood lipid activity research
Containing coronoid process dissipate capsule bacterium Fu tea extraction living, tablet, solid beverage, noodles to hyperlipidemia rats lipids in serum
Horizontal influence test.
10.1 test materials
Animal: Wistar male rat, 8~10 week old, 180~220g, are tested totally by Zhejiang Academy of Medical Sciences by 60
Animal center provides.
Experimental subjects: embodiment 2 contains coronoid process dissipate capsule bacterium Fu tea extraction, tablet, solid beverage, noodles living；It is positive right
According to medicine: blood fat recovery capsule (Beijing WBL Peking University Biotech Co., Ltd)；Cholesterol: Shanghai uncle biological Co., Ltd difficult to understand；Gallbladder
Salt: Hangzhou microorganism tries Co., Ltd.
Feed: basal feed: wheat bran 20%, soya-bean cake 20%, corn-dodger 30%, 7.9%, No. 4 powder 20% of fish meal, salt 1%,
Mineral 1%, multidimensional 0.1%.High lipid food: cholesterol 2%, cholate 0.25%, lard 8%, white sugar 2.5%, basal feed
87.25%.
10.2 test method:
Take rat 70, be randomly divided into normal group, model group, positive Effects of Xuezhikang group (each 10) and Fu tea extraction,
Tablet, solid beverage, noodles (every group 10).Normal group gives normal diet nursing daily, remaining each group is given high in fat daily
Feed (30g/ is only).After 35 days, every group is randomly selected TC, TG level in 5 survey serum, judges whether modeling succeeds.After success,
Start to be administered.Positive controls daily every is pressed 300mg/kg weight to Effects of Xuezhikang, and therapeutic component daily every is given Fu tea respectively
Extract, tablet, solid beverage, noodles extract are equivalent to Fu tea extraction 100mg/kg weight, and normal group, model group gives
Ultrapure water is given, daily every 1mL/100g weight, the above equal gastric infusion, once a day, and all animal free waters, administration 35
After it, animal blood is taken to detect.
Collection of specimens: all rats can't help water 12h in materials fasted for one day prior, and yellow Jackets are infused by the abdominal cavity 40mg/kg
Penetrate anesthesia, abdominal aortic blood prepares serum, -80 DEG C freeze it is spare.Observation item and measuring method: TC in serum (lot:
2575), TG (lot:3112), HDL-C (lot:3659) content use enzymatic assays with Olympus company kit；LDL-C
(Zhongsheng Beikong Biological Science & Technology Co., Ltd.), using the method for removing.On Olympus AU400 automatic clinical chemistry analyzer
It is operated by kit requirement.
Statistical method: it is counted with SPSS statistical software, using ANOVA variance analysis, group is used compared between group
Dunnet is examined, and is as a result indicated with mean ± SD.
10.3. result: containing coronoid process dissipate capsule bacterium Fu tea extraction living, tablet, solid beverage, noodles to hyperlipidemia rats
The influence of lipids in serum level:
TC, TG and LDL-C are significantly raised in model group rats serum, and HDL-C is substantially reduced, compared with Normal group
Have significant meaning (P < 0.05), prompts hyperlipidemia modeling success.Fu tea extraction, tablet, solid beverage, noodles can reduce
Hyperlipidemia rats serum TC, TG and LDL-C content, each group have significant (P < 0.05), can significantly increase HDL-C water
Flat (P < 0.05).Effects of Xuezhikang group can also reduce serum TC, TG and LDL-C (P < 0.05), but to raising HDL-C without effect.This examination
Test the result shows that, containing coronoid process dissipate capsule bacterium Fu tea extraction living and its Related product can make TC, TG in hyperlipidemia rats serum,
LDL-C content reduces, and illustrates that it may increase the counter transport of lipid, reach removing lipid by directly removing lipids in serum
Purpose, play adjust blood lipid effect.
Influence (n=10) of the table 2 to hyperlipidemia rats serum TC, TG, HDL-C, LDL-C
Compared with blank group: # (P < 0.05)；Compared with model group: * (P < 0.05)
11 strengthen immunity activity research of embodiment
Influence (half intracorporal method) of the Fu tea extraction to Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell
11.1 experimental methods
Mouse weighing, is randomly divided into 5 groups, normal group of (1): isometric CMC-Na solution；(2) lentinan group: 0.01g
kg-1；(3) 1 Fu tea extraction group of embodiment: 0.15gkg-1。
20% chicken erythrocyte suspension 1mL is injected intraperitoneally in every mouse.It is spaced 30min, cervical dislocation puts to death animal, faced upward position and consolidated
Due on mouse plate, abdominal skin is cut off in center, through Intraperitoneal injection physiological saline 2mL, rotates mouse plate 1min.Then abdominal cavity is sucked out to wash
Liquid 1ml, average mark are dripped on 2 glass slides, are put into the enamel box for being lined with wet gauze, and 37 DEG C of incubators of dislocation incubate 30min.It incubates
Finish, rinsed in physiological saline, to remove non-attached cell.It dries, is fixed with 1:1 acetone methanol solution, 4% (v/v)
Giemsa- phosphate buffer dyes 3min, then is dried with distilled water rinsing.Macrophage is counted under oil mirror, every piece counts 100
It is a, phagocytic percentage and phagocytic index is calculated as follows.
The number of macrophages that phagocytic percentage (%)=phagocytosis chicken red blood cell number of macrophages × 100/ counts
Chicken red blood cell sum/counting the number of macrophages for phagocytic index=swallowed
11.2 experimental results
The phagocytic function of macrophage can swallow the ability of chicken red blood cell by peritoneal macrophage to judge.As a result it shows
Show, Fu tea extraction group Turnover of Mouse Peritoneal Macrophages is above normal group (P < 0.05) to the phagocytic index of chicken red blood cell, shows
Fu tea extraction group drug has the function of promoting macrophage phagocytosis of mice.Meanwhile it being digested in observation chicken red blood cell
Degree when find, normal chicken red blood cell mostly cytoplasm chartreuse, the nuclear pyknosis organized is in hyacinthine, belongs to II grade of slight digestion；
And each light dye of administration group chicken red blood cell cytoplasm, the light light gray of karyon, belong to III grade of severe digestion；Rarely seen form in a small amount of macrophage
The vacuole of similar chicken red blood cell size, neat in edge, karyon may be seen indistinctly chicken red blood cell, belong to IV grade of digestion completely.
Influence of the 3 Fu tea extraction of table to Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell
Compared with normal group, * P < 0.05
The present invention describes preferred embodiment, and once a person skilled in the art knows basic creative general
It reads, then additional changes and modifications may be made to these embodiments.So it includes preferred real that the following claims are intended to be interpreted as
It applies example and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (10)
1. a kind of preparation method of the Fu tea extraction containing coronoid process dissipate capsule bacterium living, which is characterized in that complete the Fu tea of fermentation
For raw material, it is classified sieving or wet concentration classification sieving through low temperature drying, coronoid process dissipate capsule bacterium and tealeaves are separated；Tealeaves and water are by weight
With volume ratio, into resulting tealeaves be added the 8-12 times of water measured, room temperature extraction or refluxing extraction 2-3 times, combined extract subtracts
Pressure concentration, it is dry, obtain tealeaves primary extract；Isolated coronoid process dissipate capsule bacterium is added in tealeaves primary extract, mix to get
Fu tea extraction containing coronoid process dissipate capsule bacterium living.
2. the preparation method of the Fu tea extraction according to claim 1 containing coronoid process dissipate capsule bacterium living, which is characterized in that institute
Stating Fu tea raw material is that loose tea first disperses tea cake or brick tea if raw material form is tea cake or brick tea；Aqua Folium Camelliae sinensis Extraction technique
Are as follows: tealeaves and water are by weight with volume ratio, the water of 12 times of amounts of addition into resulting tealeaves, and refluxing extraction 2 times, every time 2 hours；
Drying means in Aqua Folium Camelliae sinensis extraction includes but is not limited to dry, be spray-dried and be freeze-dried.
3. the preparation method of the Fu tea extraction according to claim 1 containing coronoid process dissipate capsule bacterium living, which is characterized in that low
The method of temperature drying classification sieving are as follows: the Fu tea for taking dispersion is dried at not higher than 60 DEG C, and rubbing or mechanical stirring, make by hand
Coronoid process dissipate capsule bacterium is fallen from tealeaves surface, is sieved through 50-300 mesh screen multistage, is obtained coronoid process dissipate capsule bacterium thallus；
The method of wet concentration classification sieving are as follows: take Fu tea, 8-12 is added into resulting tealeaves by weight with volume ratio in tealeaves and water
The water of amount again, soaking at room temperature and by hand rubbing or mechanical stirring, falls on coronoid process dissipate capsule bacterium in water from tealeaves surface, through 50-
The sieving of 300 mesh screen multistages, obtains aqueous coronoid process dissipate capsule bacterium thallus, low temperature drying up to dry thallus, filtering gained aqueous after
Continue for extracting tealeaves.
4. the preparation method of the Fu tea extraction according to claim 3 containing coronoid process dissipate capsule bacterium living, which is characterized in that institute
Stating multistage sieving was 80 mesh, 120 mesh, 200 mesh three-levels sieve.
5. the preparation method of the Fu tea extraction according to claim 1 containing coronoid process dissipate capsule bacterium living, which is characterized in that tea
When Ye Shui is extracted, when being concentrated under reduced pressure and the technological temperature of drying process is not higher than 60 DEG C, coronoid process dissipate capsule bacterium is added in advance
In concentration or drying process technique.
6. the preparation method of the Fu tea extraction according to claim 1 containing coronoid process dissipate capsule bacterium living, which is characterized in that institute
The method of stating is also applied for the preparation of the extract containing viable bacteria for the fermentation teas that other contain coronoid process dissipate capsule bacterium.
7. the Fu tea extraction containing coronoid process dissipate capsule bacterium living that a kind of method according to claim 11 obtains.
8. a kind of Fu tea extraction as claimed in claim 7 containing coronoid process dissipate capsule bacterium living is in drug, health food, food preparation
In application.
9. the Fu tea extraction according to claim 8 containing coronoid process dissipate capsule bacterium living is in drug, health food, food preparation
In application, which is characterized in that the extract is added in the production technology of drug, health food and food, passes through control
Time point, temperature and time is added to keep the vigor of coronoid process dissipate capsule bacterium in system, obtains the product without tealeaf residue, both can guarantee and mention
The recovery rate of object is taken, keeps the dual bioactivity of coronoid process dissipate capsule bacterium and tealeaves in product, and it is uniform to greatly improve product
Degree and mouthfeel.
10. the Fu tea extraction according to claim 9 containing coronoid process dissipate capsule bacterium living is in drug, health food preparation
Using, which is characterized in that various oral drugs are made by adding supplementary material appropriate in the drug, health food and food
Dosage form, including but not limited to tablet, capsule, granule；Various food formulations, including but not limited to pressed candy, solid are made
Beverage, noodles, flour, rice flour；
Show coronoid process dissipate capsule bacterium can be made to send out containing the Fu tea extraction and Related product of coronoid process dissipate capsule bacterium living through pharmacodynamic study
Wave probiotics effect and play tea extract active material double health effect, have reduce serum total cholesterol TC,
Triglycerides TG and low-density lipoprotein LDL-C are horizontal, reduce blood viscosity, and the effect of strengthen immunity shows that it has and adjusts
It saves blood lipid, improve the effect of blood and strengthen immunity, can be used for preparing related drugs, health food and functional food.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868843A (en) * | 2021-01-29 | 2021-06-01 | 陕西科技大学 | Probiotic Fuzhuan tea beverage and preparation method thereof |
| CN114957496A (en) * | 2022-04-11 | 2022-08-30 | 湖南农业大学 | Monascus coronarius spore polysaccharide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754877A (en) * | 2016-04-12 | 2016-07-13 | 黄晓 | Method for extracting eurotium cristatum from dark tea and application thereof |
| CN106306203A (en) * | 2016-08-29 | 2017-01-11 | 刘翔 | Production method of Fu tea concentrated beverage |
-
2019
- 2019-02-27 CN CN201910147689.3A patent/CN109806320A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754877A (en) * | 2016-04-12 | 2016-07-13 | 黄晓 | Method for extracting eurotium cristatum from dark tea and application thereof |
| CN106306203A (en) * | 2016-08-29 | 2017-01-11 | 刘翔 | Production method of Fu tea concentrated beverage |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868843A (en) * | 2021-01-29 | 2021-06-01 | 陕西科技大学 | Probiotic Fuzhuan tea beverage and preparation method thereof |
| CN114957496A (en) * | 2022-04-11 | 2022-08-30 | 湖南农业大学 | Monascus coronarius spore polysaccharide and preparation method and application thereof |
| CN114957496B (en) * | 2022-04-11 | 2023-11-07 | 湖南农业大学 | Aspergillus coronatus spore polysaccharide and preparation method and application thereof |
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Application publication date: 20190528 |