CN109805172A - A kind of composite bacteria fermentation of Chinese herbal medicine feed and preparation method thereof - Google Patents
A kind of composite bacteria fermentation of Chinese herbal medicine feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of composite bacteria fermentation of Chinese herbal medicine feeds, are made of the fermenting raw materials of following weight percent: corn 20~30%, wheat bran 29.9~39.9%, phytase 0.1%, stomach invigorating tangerine peel powder 5~10%, red yeast rice hawthorn powder 5~10%, composite bacteria dilution 5%, water 20%.The preparation method of composite bacteria fermentation of Chinese herbal medicine feed, after above-mentioned raw materials are proportionally removed impurity, it is crushed into pulverizer, enter mixing machine after crushing and carries out dry material mixing, after mixing to material, thermostatted water in liquid agitation tank is sprayed into mixing machine, the composite bacteria dilution after activating in activation pot is sprayed into mixing machine simultaneously and is mixed.It passes through the metabolism of beneficial bacterium, degradation part non-digestible high molecular weight protein, fat and polysaccharide etc., the metabolite of the small molecules nutriments and beneficial bacterium such as soluble polypeptide, organic acid is generated, acquisition palatability is good, full of nutrition, beneficial bacterium viable count is high.
Description
Technical field
The invention belongs to technical field of microbial fermentation more particularly to a kind of composite bacteria fermentation of Chinese herbal medicine feed and its systems
Preparation Method.
Background technique
With the development of large-scale cultivation industry, the harm that antibiotic is widely used in breeding process increasingly shows, and livestock and poultry produce
Product become safely the focus of social concerns.The use of antibiotic is reduced, is added in feed until completely forbidding antibiotic,
As China animal husbandry development there is an urgent need to.
Fermentative feedstuff of microbe generallys use two kinds of technology patterns of solid state fermentation and liquid state fermentation at present.Although two kinds of techniques
It has their own advantages, but the technological deficiencies such as the former there are fermentation processes hardly possible, labor intensity is high, and fermentation loss is big;Latter devices at
This is big, is not suitable for production method of China's breeding enterprise based on " from ingredient ".Using respiratory membrane pocket type fermentation technique, Ke Yiyou
Effect reduces producing cost, improves fermented feed production efficiency, and easy to operate.
Common fermenting microbe is mainly bacillus, lactic acid bacteria and saccharomycete in production at present.Conventional strain compounding
The anti-nutritional factors in raw material can be decomposed or be converted, generation can be by livestock and poultry using the growth metabolism activity of microorganism itself
Feeding, absorbs digestion, and nutrient is higher and the feedstuff of nonhazardous effect, improves feed nutrition and absorbs horizontal, degradation feed is former
Toxin that may be present in material.
But how reasonably combined strain, generate more preferably using effect using respiratory membrane pocket type fermentation technique, still have compared with
Big researches and develops space.
Summary of the invention
It is above-mentioned to solve the purpose of the present invention is to provide a kind of composite bacteria fermentation of Chinese herbal medicine feed and preparation method thereof
Technical problem.
To achieve the above object the invention adopts the following technical scheme:
A kind of composite bacteria fermentation of Chinese herbal medicine feed, is made of the fermenting raw materials of following weight percent: corn 20~
30%, wheat bran 29.9~39.9%, phytase 0.1%, stomach invigorating tangerine peel powder 5~10%, red yeast rice hawthorn powder 5~10%, compound bacteria
Kind dilution 5%, water 20%.
As a further solution of the present invention, the composite bacteria dilution is saccharomyces cerevisiae, bacillus subtilis, lactic acid
The composite bacteria agent A of bacterium and the composite bacteria agent B of clostridium butyricum, bacillus licheniformis are mixed.
A kind of preparation method of the composite bacteria fermentation of Chinese herbal medicine feed, include the following steps: proportionally by corn,
After wheat bran, phytase, stomach invigorating tangerine peel powder, red yeast rice hawthorn powder removal impurity, is crushed into pulverizer, mixing is entered after crushing
Machine carries out dry material mixing, after mixing to material, the thermostatted water in liquid agitation tank is sprayed into mixing machine, thermostatted water is worked as
It is sprayed onto total amount of water half, the composite bacteria dilution after activating in activation pot is sprayed into mixing machine simultaneously and is mixed, i.e.,
It can.
As a further solution of the present invention, the composite bacteria dilutes liquor manufacture method: by 1Kg saccharomyces cerevisiae, withered grass
The composite bacteria agent A of bacillus, lactic acid bacteria, wherein contain saccharomyces cerevisiae, 1.0 × 108CFU/g;Bacillus subtilis, 1.0 ×
108CFU/g;Enterococcus faecalis dry powder, 1.0 × 107CFU/g, with the clostridium butyricum of 0.5Kg and the composite bacteria agent of bacillus licheniformis
B, wherein containing clostridium butyricum 2 × 109CFU/g, bacillus licheniformis 5*1010CFU/g, bacillus subtilis 5*1010CFU/g is mixed
Composite bacteria agent is made after conjunction;
20Kg brown sugar is dissolved into 50Kg40 DEG C of warm water, composite bacteria agent is added in brown sugar water after filtering and at the uniform velocity stirs 5
Minute, heat preservation stands 30 minutes, makes strain rapid fluid resuscitation.
As a further solution of the present invention, dry material mixing incorporation time is 3.5min.
As a further solution of the present invention, 40 DEG C of thermostatted waters spray into mixing in 39-41 DEG C of liquid agitation tank of thermostat temperature
In machine.
As a further solution of the present invention, after remixing 1.0min after all liq penetrating mixing machine, mixing machine is opened
Material after inoculation mixing is discharged into finished bin by discharge door, and finished bin divides into two spiral discharging machines, and every discharging machine is by riding
Switch control discharging, is measured by electronic platform scale and is weighed, and the bag film after weighing completes the life of fermented feed after being sealed by heat sealing machine
It produces, the fermented feed after sealing is stored in the fermenting cellar of 26 degrees Celsius of 50-80% humidity and fermented 7 days.
The beneficial effects of the present invention are: the fermentation of (1) traditional microbiological dry method is all first to be made into product, then pack.Before packaging
To pass through drying and processing, what final animal was had is that high temperature kills " bacterium corpse ".Respiratory membrane pocket type antimicrobial composition anaerobism solid-state
Zymotechnique is that packaging bag is designed to small-sized fermentation tank, using first packaging post-fermentation technique.Drying and processing is not needed, is finally
Animal is supplied in the form of viable bacteria.
(2) the specific microorganism fungus kind combination developed can be such that lactic acid bacteria and clostridium butyricum breeds rapidly, and it is excellent to occupy quantity
Gesture.Ingenious the advantages of combining anaerobic bacteria and aerobic bacteria fermentation, raw material, which does not need sterilizing, can be directly used in inoculated and cultured.
(3) fermented feed that is incorporated as of Chinese herbal medicine imparts abundanter effect, and hawthorn has food digesting stomach fortifying, promoting blood circulationization
The effect of silt, convergence and dysentery, dried orange peel plays the role of strengthening the spleen and stomach, ventilating, lowering the adverse-rising QI and eliminating phlegm.The Chinese herbal medicine of two kinds of integration of drinking and medicinal herbs
It is added, while the use for composite bacteria of arranging in pairs or groups can utilize its secondary metabolite abundant to greatest extent.Significantly improve poultry
Fowl intestinal health enhances livestock and poultry digestive function, reduces feedstuff-meat ratio, improves the price of deed.
(4) due in fermented feed the content of probiotics and prebiotics it is higher, large dosage addition after can improve animal rapidly
Intestinal microflora improves feed digestibility, reduces enteral nutrition material concentration, increases hind microflora diversity, can be reduced
Trophism, bacterial diarrhea, and effect is obvious.This product uses in young animal feed, can complete substitute antibiotics, produce
The raw effect for improving production performance and reducing diarrhea, livestock and poultry entirety disease resistance are promoted.
(5) fermented feed palatability is good, is rich in organic acid, sow feed intake can be improved, while reducing intestinal pH, stimulates intestines
Road is wriggled, and is accelerated defecation speed, is found through experiments that, can be obviously improved gestation within 1 week using the fermented feed that do not dry, be fed
The constipation situation of newborn phase sow.
(6) eggshell quality and content of microorganisms in birds cloaca are closely related, and a large amount of probiotics can show in fermented feed
Reduce the total amount of enteron aisle paracolon, have obvious effect to the reduction of cloaca quasi-microorganism total amount, therefore can be in 7~15d
Inside observe the variation of eggshell quality.
(7) there are a large amount of microorganisms in ruminant tumor gastric, yeast class fermented feed can improve rumen microflora, mention
The digestion and absorption of high microsteping class feed is found by the fermented feed for adding 10% in meat sheep feed, can be mentioned in 10~15d
High 5%~10% fiber-digesting capacity.
(8) by adding this fermented feed, the digestibility of animal entirety can be improved, it is dense to reduce nutriment in excrement
Degree, to reduce the discharge of ammonia, sulphur, generally ammonia concentration in breeding environment, which can be observed, in 2~3d of addition is reduced.
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
The present invention is further elaborated in the following with reference to the drawings and specific embodiments.
The preparation of fermented feed:
The preparation of strain dilution and activation: the composite bacteria agent 1 of saccharomyces cerevisiae, bacillus subtilis, lactic acid bacteria (is contained into wine brewing
Yeast, 1.0 × 108CFU/g, bacillus subtilis, 1.0 × 108CFU/g, enterococcus faecalis dry powder, 1.0 × 107CFU/g) 1Kg with
Clostridium butyricum and the composite bacteria agent of bacillus licheniformis 2 (contain clostridium butyricum 2 × 109CFU/g, bacillus licheniformis 5*1010CFU/
G, bacillus subtilis 5*1010CFU/g composite bacteria agent) is made after 0.5Kg mixing.
20Kg brown sugar is dissolved into 50 kilograms of 40 DEG C of warm water, composite bacteria agent is added in brown sugar water after filtering and at the uniform velocity stirs
5 minutes, heat preservation stood 30 minutes, made strain rapid fluid resuscitation.Composite bacteria agent 1 is educated up to the bright Asia in Beijing purchased from the bright Asia in Beijing and is educated up to biology
Science and Technology Ltd., composite bacteria agent 2 are purchased from Guangdong great Ze agriculture Biotechnology Co., Ltd.
Fermentation raw material preparation: raw material is put into according to following proportion
Material name | Weight Kg |
Corn | 250 |
Wheat bran | 399 |
Phytase | 1 |
Stomach invigorating tangerine peel powder | 50 |
Red yeast rice hawthorn powder | 50 |
Water | 200 |
Composite bacteria dilution | 50 |
It is total | 1000 |
As shown, 399 kilograms of wheat brans, 1 kilogram of phytase, 50 kilograms of stomach invigorating tangerine peel powders, 50 is public by 250 kilograms of corns
Jin red yeast rice hawthorn powder puts into feed inlet in order, and pulse dust collector (101) one are set at feed inlet can directly remove charging dust,
Through grizzly enter bucket elevator (102) be promoted to cleaning screen (103), pipe magnetic separator (104) remove impurity it is laggard enter storehouse to be comminuted
(105), material is crushed by Manual flashboard control into pulverizer (106) again in storehouse to be comminuted, and pulse is set by pulverizer and is removed
Dirt device (107) assists air draught, and crushing rear material is delivered to bucket elevator (109) by screw conveyor (108) and is promoted to spiral
Mixing machine (110) carries out dry material mixing.After mixing (incorporation time 3.5min) to material, Liquid spraying pump (117) are opened, it will
40 DEG C of thermostatted waters spray into mixing machine in constant temperature (39-41 DEG C) liquid agitation tank (114), when thermostatted water is sprayed onto total amount of water half
(100 kilograms) Shi Kaiqi strain pumps (116), and the strain dilution (50 kilograms) after activation in activation pot (115) is sprayed into simultaneously
It is mixed in mixing machine, after remixing one minute after all liq penetrating mixing machine, opens discharging door of mixer and mix inoculation
Material is discharged into finished bin (111) after conjunction, and finished bin divides into two spiral discharging machines (112), and every discharging machine is by floor push
Control discharging, is measured by electronic platform scale (113) and is weighed, and the bag film after weighing completes the life of fermented feed after being sealed by heat sealing machine
It produces.
Fermented feed after sealing is stored in the fermenting cellar of 26 degrees Celsius of 50-80% humidity and is fermented 7 days.
The living stems result of fermentation of Chinese herbal medicine feed product
Influence of the fermented feed to growing and fattening pigs feedstuff-meat ratio:
Child care piglet of 39 weight at 35-40 kilograms is selected, is randomly divided into 4 processing by weight phase approximately principle, each
2 repetitions are handled, it is each to repeat 4-6 piglets.Basal diet composition and trophic level are shown in Table 1.Pre-feeding period is 7d, and experimental period is
15d.Piglet is freely eaten and drinks water using being fed in different sheds.Pig is by the grand live pig of Shaanxi Condar that agriculture and animal husbandry Science and Technology Ltd.
Breeding base provides.
Group | Strain |
A | Saccharomycete+lactic acid bacteria+bacillus |
B | Saccharomycete+lactic acid bacteria+bacillus+clostridium butyricum |
C | Saccharomycete+lactic acid bacteria+withered grass, bacillus licheniformis+clostridium butyricum+monascus ruber |
D | Blank control group |
From the experimental results, it is below blank control group using A, B, C group feedstuff-meat ratio FCR of fermented feed, illustrates to ferment
The use of feed can improve pig to the utilization rate of feed, and use the pig of C group fermented feed, and enteron aisle is more healthy, feed
Utilization rate is higher.It is calculated according to stage feedstuff-meat ratio, feedstuff-meat ratio reduces by 0.2, delivers for sale according to 110 kilograms, every pig can save
22 kg diet of feed, i.e. every pig can get a profit about 66 yuan more.
Influence of the fermented feed to diarrhea piglet:
It selects 30 age in days average weights in 7.1kg Ternary Pig weaning brash piglet 27, divides at random by weight phase approximately principle
It is handled for 3,9 repetitions of each processing, 1 piglet of each repetition.Diet Formula is shown in Table 1.Experimental period is 5d.Piglet, which uses, divides
Column raising is freely eaten and drinks water.Diarrhea piglet quantity is counted after experimental period.Pig is had by your agriculture and animal husbandry science and technology of Shaanxi Condar
The grand live pig Breeding base of limit company provides.
Table 1. tests Diet Formula and nutrient content
Project | Blank control group | 4% fermented feed group | 8% fermented feed group |
Corn | 60.000 | 60.000 | 60.000 |
Imported fish meal | 6.000 | 6.000 | 6.000 |
Dregs of beans | 12.000 | 12.000 | 12.000 |
Expanded soybean | 10.000 | 10.000 | 10.000 |
Wheat bran | 8.000 | 4.000 | |
Core material | 4 | 4 | 4 |
Fermented feed | 4 | 2 | |
It is total | 100.00 | 100.00 | 100.00 |
CP% | 17.44 | 17.33 | 17.39 |
DE Kcal/Kg | 3354.70 | 3355.36 | 3342.42 |
EE% | 4.74 | 4.76 | 4.78 |
CF% | 2.13 | 2.07 | 2.12 |
Ca% | 0.55 | 0.55 | 0.55 |
AP% | 0.35 | 0.34 | 0.34 |
Lys% | 1.17 | 1.16 | 1.17 |
Met% | 0.35 | 0.35 | 0.35 |
Cys% | 0.33 | 0.33 | 0.33 |
Met+Cys% | 0.68 | 0.68 | 0.68 |
Thr% | 0.76 | 0.76 | 0.76 |
Trp% | 0.22 | 0.22 | 0.22 |
Table 2 adds influence of the fermented feed to grice diarrhoea
Note: 1d is to start to feed the fermentation material date
Table 3 adds influence of the fermented feed to grice diarrhoea percentage
Group | 1st day | 2nd day | 3rd day | 4th day | 5th day |
Control group | 88.89% | 88.89% | 66.67% | 77.78% | 55.56% |
4% fermented feed group | 66.67% | 44.44% | 11.11% | 0.00% | 0.00% |
8% fermented feed group | 87.50% | 87.50% | 75.00% | 37.50% | 0.00% |
By table 2,3 it is found that reloading the 1st day, the grice diarrhoea head number of two groups of testing materials declines, but unobvious;?
It reloads the 2nd day, the 3rd day, 4% fermented feed group grice diarrhoea head number down ratio is most fast;It is reloading the 4th day, 4% fermented feed
Group grice diarrhoea head number has been reduced to 0, and the 5th day, test group piglet diarrhea was reduced to 0, and piglet is always during test for control group
There are diarrhea, and ratio reduction is very slow.
Influence of the fermented feed to meat chicken growth performance:
One test objective: effect of the research fermented feed to meat chicken growth performance;
Two experimental designs:
It chooses 120 1 age in days Sa rope broiler chicken and is randomly divided into 4 processing groups, every group of 3 repetitions, 10 chickens of each repetition,
1-21d feeds chicken material, the 22-35 days big chicken material of feeding.
The test grouping of table 1
Control group | 2% fermented feed group | 4% fermented feed group | 8% fermented feed group |
Basal diet | + 2% fermented feed of basal diet | + 4% fermented feed of basal diet | + 8% fermented feed of basal diet |
2 feed formula of table
Test result:
Influence of 3 fermented feed of table to meat chicken growth performance
The Europe EPEF- performance index=[survival rate (%) * weight (kg)/deliver number of days * feed-weight ratio for sale] Europe * 10000EBI-
Continent broiler chicken index=[survival rate (%) * average daily gain (g)/feed-weight ratio] * 10.
It can be seen that, each group voluntary feed intake is without significant difference when 35d from table 3, but compared with the control group, 2% fermentation
2.91%, 5.38% and 3.12%, and 4% fermentation is respectively increased in the body weight gains of material group, 4% fermentation material group and 8% fermentation material group
The feed-weight ratio of material group significantly reduces.The European performance index (EPEF) of test group and European broiler chicken index (EBI) also significantly improve,
Survival rate improves 6.67% than control group.
Influence of the fermented feed to laying period performance in layers:
Test objective: influence of the verifying fermented feed to laying period performance in layers;
Experimental design: being divided into two groups for 280 age in days laying hens, every group of 700 plumages, feed respectively basal diet (control group) and
+ 4% fermented feed of basal diet (4% fermented feed group).10 weeks by a definite date.Detailed accurate recording daily ingestion amount after on-test,
Laying rate, egg size.
1 basal diet formula of table
Ingredient | Chicken material % | Assay value and calculated value | Chicken material |
Corn | 62 | Metabolic energy (Kcal/kg) | 2650 |
43% dregs of beans | 21 | Thick protein % | 22.92 |
Wheat bran | 5.2 | Lysine % | 0.8 |
Soya-bean oil | 1 | Methionine % | 0.42 |
Mountain flour | 9 | Calcium % | 3.75 |
Calcium monohydrogen phosphate | 0.75 | Available phosphorus % | 0.26 |
70% lysine | 0.45 | NaCl% | 0.15 |
DL- METHIONINE | 0.12 | ||
Vitamin and mineral premix | 0.3 | ||
NaCl | 0.18 | ||
It is total | 100 |
Influence of 2 fermented feed of table to performance in layers
Project | Control group | 4% fermented feed group |
Laying hen number, plumage | 700 | 700 |
Average egg number, piece/day | 648 | 654 |
Experimental period average egg production, % | 92.71 | 93.57 |
Feed intake, g | 120 | 121 |
The death rate, % | 1.1(8) | 0.86(6) |
Experimental period average egg weight, g | 63.82 | 63.84 |
From table 2 it can be seen that the average egg production of 4% fermented feed group is compared with control group height, the death rate is low compared with control group, adopts
Appetite and average egg weight do not have difference.Illustrating, the laying rate of laying hen can be improved in addition fermented feed, and it reduces the dead of laying hen and washes in a pan,
But not influence the feed intake and egg size of laying hen.
The above is present pre-ferred embodiments, for the ordinary skill in the art, according to the present invention
Introduction, in the case where not departing from the principle of the present invention and spirit, changes, modifications, replacement and change that embodiment is carried out
Type is still fallen within protection scope of the present invention.
Claims (7)
1. a kind of composite bacteria fermentation of Chinese herbal medicine feed, which is characterized in that be made of the fermenting raw materials of following weight percent: beautiful
Rice 20~30%, wheat bran 29.9~39.9%, phytase 0.1%, stomach invigorating tangerine peel powder 5~10%, red yeast rice hawthorn powder 5~10%,
Composite bacteria dilution 5%, water 20%.
2. a kind of composite bacteria fermentation of Chinese herbal medicine feed as described in claim 1, which is characterized in that the composite bacteria dilution
Liquid is the composite bacteria agent B of saccharomyces cerevisiae, bacillus subtilis, the composite bacteria agent A of lactic acid bacteria and clostridium butyricum, bacillus licheniformis
It is mixed.
3. a kind of preparation method of the composite bacteria fermentation of Chinese herbal medicine feed as described in claim 1-2 is any, which is characterized in that packet
Include following steps: after corn, wheat bran, phytase, stomach invigorating tangerine peel powder, red yeast rice hawthorn powder are proportionally removed impurity, into powder
Broken machine is crushed, and mixing machine progress dry material mixing is entered after crushing after mixing to material will be in liquid agitation tank
Thermostatted water sprays into mixing machine, when thermostatted water is sprayed onto total amount of water half, by the composite bacteria dilution after activating in activation pot
It sprays into mixing machine and is mixed simultaneously.
4. the preparation method of composite bacteria fermentation of Chinese herbal medicine feed as claimed in claim 3, which is characterized in that the compound bacteria
Kind dilution liquor manufacture method: by 1Kg saccharomyces cerevisiae, the composite bacteria agent A of bacillus subtilis, lactic acid bacteria, wherein contain saccharomyces cerevisiae,
1.0×108CFU/g;Bacillus subtilis, 1.0 × 108CFU/g;Enterococcus faecalis dry powder, 1.0 × 107CFU/g, with 0.5Kg's
The composite bacteria agent B of clostridium butyricum and bacillus licheniformis, wherein containing clostridium butyricum 2 × 109CFU/g, bacillus licheniformis 5*
1010CFU/g, bacillus subtilis 5*1010Composite bacteria agent is made after mixing in CFU/g;
20Kg brown sugar is dissolved into 50Kg40 DEG C of warm water, composite bacteria agent is added in brown sugar water after filtering and at the uniform velocity stirs 5 minutes,
Heat preservation stands 30 minutes, makes strain rapid fluid resuscitation.
5. the preparation method of composite bacteria fermentation of Chinese herbal medicine feed as claimed in claim 3, which is characterized in that dry material mixing
Incorporation time is 3.5min.
6. the preparation method of composite bacteria fermentation of Chinese herbal medicine feed as claimed in claim 3, which is characterized in that thermostat temperature
40 DEG C of thermostatted waters spray into mixing machine in 39-41 DEG C of liquid agitation tank.
7. the preparation method of composite bacteria fermentation of Chinese herbal medicine feed as claimed in claim 3, which is characterized in that all liq spray
Enter after remixing 1.0min after mixing machine, opening discharging door of mixer material will be discharged into finished bin after inoculation mixing, finished bin
Two spiral discharging machines are divided into, every discharging machine is controlled by floor push and discharged, and is measured and is weighed by electronic platform scale, thin after weighing
Film bag completes the production of fermented feed after being sealed by heat sealing machine, fermented feed after sealing is in 26 degrees Celsius of 50-80% humidity
It stores and ferments 7 days in fermenting cellar.
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