CN109802984A - Intelligent agricultural and environmental management system - Google Patents
Intelligent agricultural and environmental management system Download PDFInfo
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- CN109802984A CN109802984A CN201711148098.5A CN201711148098A CN109802984A CN 109802984 A CN109802984 A CN 109802984A CN 201711148098 A CN201711148098 A CN 201711148098A CN 109802984 A CN109802984 A CN 109802984A
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Abstract
The present invention relates to a kind of intelligent agricultural and environmental management systems, include monitoring unit, transmission unit, computing unit, control unit and target substance administration unit.Monitoring unit collects the firsthand information in monitoring region middle ring border；Transmission unit is mutually coupled with monitoring unit, to receive and transmit firsthand information；Computing unit is mutually coupled with transmission unit, to receive the firsthand information from transmission unit, and is selected target substance in target substance data bank according to tactful algorithm and is concluded overhead；Control unit is mutually coupled with computing unit, to receive overhead and generate control signal；And target substance administration unit is connected with described control unit, to receive control signal, and one or more target substances of predetermined concentration and predetermined amount is applied according to control signal in monitoring region.
Description
Technical field
It is espespecially a kind of to carry out application target according to ambient condition the present invention relates to a kind of intelligent agricultural and environmental management system
The intelligent agricultural and environmental management system of substance.
Background technique
Intelligent agricultural is technology (the Information and Communication using " information and communication science and technology "
Technology, ICT) and the technology of " automation equipment " to improve the situation and problem of current agricultural plant development, and combine
Traditional agriculture technology mode and concept, to improve production efficiency and produce the agricultural product of high additive value as target.
Due to universal, Internet of Things (the Internet of of entity article and information communication link of Smartphone
Things, IoT) science and technology starts to flourish.According to the concept of Internet of Things, entity article that is different and separating can pass through it is wired or
Wi-Fi to detect, monitor immediately, operation, processing, communication and executes various related works, and therefore various with applying to
The potentiality in field.
Intelligent agricultural (Smart Farming) is to combine big data analysis, detection technology, precision agriculture, intelligence machine
The technologies such as device, Internet of Things and Machine To Machine (Machine to Machine, M2M), the accurate mode for adjusting cultivation management.
Climatic variation, water resource shortage, insect pest, warm up effect, novel pest and disease damage under the influence of, according to soil and crop situation, pour
The number that the amount and water for drinking chemical fertilizer are sprayed, or utilization global positioning system (Global Positioning System,
GPS the device of tractor is manipulated) to realize large-scale production.Intelligent agricultural will make agricultural production wisdom.However this skill
Art needs huge fund and the talent, the collection of precise information and the cooperation of the relevant technologies.
Intelligent agricultural broadly includes: the technology of agricultural informatization (Agri-Informatics, AI) and accurate agricultural,
That is, application Internet, information information, cloud operation (Cloud Computing), distal end manipulation, automation equipment and
General soft or hard body technique.Its object is to save labour and mass production, performance crop greatest limit, the peace for ensuring staff
Full operating environment and offer consumer and trust sense of the actual demand person for food safety (the offer end in material source).
In agricultural or environmental management, such as chemical fertilizer, chemical products, Insecticides (tech) & Herbicides (tech), hormone are usually faced
Class and the excessive selection for adjusting the chemical substances such as class starter, lead to the pollution of surface water and underground water, become agricultural and ring
Border scientific domain problem of interest.The chemically object that therefore, it is necessary to be accurately controlled in environment to be applied to or on crop
The concentration and dosage of matter.Therefore, pesticide, toxin or fertilizer etc. of biological victory peptide or protein matter have smaller residual,
Substance friendly to environment and to particular species tool specificity, just gradually developed with replace traditional pesticide, chemically pesticide,
Toxin or fertilizer etc..
Biological pesticide or biological agent, such as: natural materials pesticide, microbial pesticide, biochemical pesticide and genetic engineering
Technology produces the microbial pesticide of system, via animal, plant, microorganism and its derivative product, is also gradually developed existing to replace
Some pesticides.
Microorganism formulation refer to for crop cause of disease, pest, control of weeds or induce crop resistance microorganism or its have
Imitate the ingredient product made via formula, source includes bacterium, fungi, virus and protozoan, generally can be via nature
Boundary separate and obtains, or can again via artificial mutagenesis, eliminate choosing or gene be transformed etc. technologies improved, Jin Erqu
For existing pesticide.
Biochemical preparation refers to that biochemical pesticide includes that insect pheromones (pheromone) etc. are pure by chemistry with biological material
It takes or synthesizes, mechanism of action nonhazardous person, such as: beet armyworm property takes Lip river and prodenia litura property pheromones, is also gradually developed
To replace existing pesticide.
Natural materials include nicotine (nicotine), pyrethrosin (pyrethrum), derris extract (rotenone), black false hellebore
The natural products such as alkali (sabadilla, vertine), print chinaberry (azadirachtin), plain saponin (saponins), not with chemistry
Method refines or is closed again winner.
However, biological victory peptide or protein matter is easier to natural degradation compared to chemical substance, thus it is difficult to grasp it
Selection amount between field or environmental area, and need a large amount of manpowers to track and manually select such biological victory peptide or protein
Matter.Therefore, it is necessary to the concept of Internet of Things, development can more precisely and more easily in Regulate Environment required degree chemistry
Property substance, also or it is biological victory peptide device or method.
Summary of the invention
In view of above-mentioned existing technological deficiency, inventor improves it, and research obtains the present invention accordingly.
The present invention provides a kind of intelligent agriculture and environmental management system, including monitoring unit, transmission unit, calculating list
Member, control unit and target substance administration unit, wherein original money of the monitoring unit to collect monitoring region middle ring border
Material；The transmission unit is mutually coupled with the monitoring unit, to receive and transmit the firsthand information；The computing unit with
The transmission unit mutually couples, receiving the firsthand information from the transmission unit, and according to tactful algorithm in mesh
Mark selects target substance in material goods library, and degradation mathematical model according to the target substance and the firsthand information are returned
Receive overhead；Described control unit is mutually coupled with the computing unit, to receive the overhead and generate control news
Number；And the target substance administration unit is connected with described control unit, to receive the control signal, and according to institute
It states control signal and selects the target substance of predetermined concentration and predetermined amount in the monitoring region.
Intelligent agricultural and environmental management system provided by embodiment according to the present invention, can monitor and application environment is joined
Number carrys out operation and speculates the type that need to select substance, opportunity, quantity, degree and period, and selects target substance in agricultural according to this
In environment.Therefore, intelligent agricultural and environmental management system provided by the embodiment of the present invention are simplified or improve selection mesh
Mark substance time needed for environment and manpower.In addition, intelligent agricultural and environmental management system can allow various possible choosings
For the target substance in the agricultural environment come using, increase it is intelligent agricultural and environmental management system application.
Detailed description of the invention
Fig. 1 shows the schematic diagram of intelligent agricultural and environmental management system of the invention.
Fig. 2 shows the lauryl sodium sulfate polypropylene of Hv1a (protein molecular weight 5.3kDa) and protein labeling
Acrylamide gel electrophoretogram.
Fig. 3 shows Hv1a- agglutinin (Hv1a-lectin, HL, protein molecular weight 17.1kDa) and protein mark
The sodium dodecyl sulfate polyacrylamide gel electrophoresis figure of note.
Fig. 4 shows after trypsin acting one day, Hv1a (protein molecular weight 5.3kDa) and protein labeling
Sodium dodecyl sulfate polyacrylamide gel electrophoresis figure.
Fig. 5 shows after trypsin acting one day, Hv1a- agglutinin (HL, protein molecular weight 17.1kDa) and
The sodium dodecyl sulfate polyacrylamide gel electrophoresis figure of protein labeling.
Fig. 6 shows the double reciprocal plot of the trypsin degradation rate of Hv1a (solid line) and Hv1a- agglutinin (dotted line).It is horizontal
Axis represents the inverse by matter concentration, and the longitudinal axis represents the inverse of rate.The inverse and y of the tropic and intercept the representative-KM, p of x-axis
The intercept of axis then represents Vm, the inverse of p.
Fig. 7 A to Fig. 7 F shows the simulation drawing for the degradation rate that ultraviolet radiation decomposes under different n values.Wherein, horizontal axis
The time is represented, the longitudinal axis is represented relative to initial value, remaining percentage.
Fig. 8 shows degradation curve figure of four kinds of protein in 2 hours, and wherein horizontal axis represents the time, and the longitudinal axis represents surplus
Remaining protein concentration.
Fig. 9 shows that Hv1a- agglutinin is 286nm in wavelength and intensity is 36.4mW/m2Ultraviolet light under, model
Prediction curve figure compared with experimental result.
Figure 10 shows the signal of the targeted insect counting sensing device of the intelligent agricultural of the present invention and environmental management system
Figure.
Figure 11 A and Figure 11 B show the intelligent agricultural and the signal of environmental management system of another embodiment of the present invention
Figure.
Figure 12 A and Figure 12 B show the intelligent agricultural and the signal of environmental management system of another embodiment of the present invention
Figure.
Figure 13 shows the intelligent agricultural of another embodiment of the present invention and the schematic diagram of environmental management system.
Symbol description
1,2,3,4,5: intelligent agricultural and environmental management system
10: monitoring region
20: monitoring unit
20 ': main monitoring unit
30: control unit
40: target substance administration unit
40 ': major heading substance administration unit
50: cloud
60: far-end operation unit
65: computing unit
100: control signal
200: manual control signal
300: overhead
400: operation interface
500,600: transmission network
15: firsthand information
25,35,35 ', 55: transmission unit
45: target substance
2000: targeted insect counts sensing device
210: cylinder
220: channel
230: accommodating device
230 ': accommodating device
240: infrared counter
231: insect attraction light source
232: targeted insect attractant
233: pheromones GCMS computer activates neuropeptide
234: barrel
241: RF transmitter
242: infrared receiver
250: signal processor and charge storage device.
Specific embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way
The present invention.Non-limiting examples shown in below with reference to the accompanying drawings more fully explain aspect of the invention and its specific
Feature, advantage and details.The explanation to known materials, manufacture tool, process technique etc. is omitted, in order to avoid in detail need not
The fuzzy present invention in strategic point.It should be understood, however, that the detailed description and specific example to illustrate aspect of the present invention are only used as and show
Example, rather than limit.Those skilled in the art will understand the spirit and/or range in the concept of the invention on basis from the present invention
Interior various substitutions, modification, addition and/or layout.
Approximating language used in description of the invention and claims can be used to modify arbitrarily quantitative expression,
The variation that can be changed within the allowable range without will lead to relative basic function.Therefore, by one or more term examples
As the value of " about " modification is not limited to specified exact value.In some instances, which can correspond to measured value
The precision of instrument.
Term used in the present invention is only to be not intended to the limitation present invention for the purpose for illustrating specific examples.Unless
It is explicitly pointed out in context, otherwise "one" and "the" of singular used herein above are also intended to include plural form.Also
(and any form of include), " having " (and any form of have) and "comprising" it should be appreciated that term " includes "
(and any form of include) is all open connection verb.Therefore, " comprising ", " having " or "comprising" one or more step
Rapid or element method or apparatus has those one or more steps or element, but be not limited to only to have those one or
Multiple steps or element.Similarly, the step of a kind of method of " comprising ", " having " or "comprising" one or more features or one
The element of kind device has those one or more features, but is not limited to only have those one or more features.Moreover,
The device or structure configured in a specific way at least configures by that way, but can also configure in ways that are not listed.
When term " connection " used in the present invention is used for two physical components, refer to straight between two physical components
It connects in succession.In addition, can refer to two physical components is to be directly connected to or pass through when term " coupling " is used for two physical components
The connection of one or more intermediary elements.
Term "available" used in the present invention and " may be " indicate a possibility that occurring under a set of conditions；Have
Specific attribute, characteristic or function；And/or another verb of modification, by expressing one kind associated with the modification verb
Or the mode of multi abilities, function or possibility is modified.Accordingly, it is considered to which the term being modified can in some cases
Can it is sometimes inappropriate, can not or it is improper, the use of "available" and " may be " indicates that the term being modified is obviously appropriate
, it is competent or suitable shown in performance, function or purposes.For example, in some cases, event or performance it is contemplated that and
In other cases, the event or performance can not occur, this difference is embodied by term "available" and " may be ".
With reference to the accompanying drawings, to be conducive to understand, those attached drawings are not necessarily drawn to scale, wherein are made in different attached drawings
Similar elements symbol indicates same or similar element.
Fig. 1 shows intelligent agricultural and environmental management system (1) of the invention, and it includes monitoring unit (20), transmission are single
First (25,35,35 '), computing unit (65), control unit (30) and target substance administration unit (40).In intelligent agricultural
And in environmental management system (1), monitoring unit (20) can detect the firsthand information (15) of environment in monitoring region (10).It is general and
Speech, the firsthand information (15) include type, quantity, the target of age number of one or more targeted insects in monitoring region (10)
Insect data, ultraviolet ray intensity, and at least climatological data being outside one's consideration in addition to ultraviolet light intensity.Here, by the judgement of age number,
So that the life cycle of various targeted insects is also classified as parameter, and then the kind that current and following targeted insect is illustrated can be derived
Class, quantity and age number, therefore the type and concentration for the target substance selected needed for judging that can be more convenient for.In addition, preferably real one
It applies in example, the ultraviolet ray intensity includes UVB ultraviolet ray intensity, UVA ultraviolet ray intensity or UVC ultraviolet ray intensity.
Firsthand information (15) includes monitoring at least one of region (10) climatological data, such as: air pressure, average gas pressure,
The highest temperature, the lowest temperature, temperature on average, highest humidity, minimum humidity, medial humidity, rainfall, average rainfall, moment
Rainfall, wind speed, wind direction, fog distributed degrees, haze distributed degrees, illumination, average time illumination, soil moisture, various gas
The data such as bulk concentration.This will be described in further detail below, and these being merely to illustrate property of example purposes are not for limitation sheet
Invention.
Monitoring unit (20) and transmission unit (25) couple, and monitor in monitoring unit (20) and collect firsthand information (15)
Afterwards, transmission unit (25) carries out data reception and transmission, wherein transmission unit (25) includes radio communication network or wire communication net
Road.
Monitoring unit (20) passes firsthand information (15) after monitoring and collecting firsthand information (15), by radio communication network
Transport to cloud (50).After firsthand information (15) are transmitted and are received by computing unit (65), computing unit (65) can be based on original
Data (15) selects one or more target substances for targeted insect according to tactful algorithm in target substance data bank
(45).Here, target substance data bank can be the data bank being built into computing unit (65), or computing unit (65) coupling can be made
The data bank being connected in any other device, unit or system, and the invention is not limited thereto.
The foundation of the target substance data bank can be collected via crawler (Crawler) step logs in various victory peptides to be selected
In the target substance data bank, and at least records the base-pair quantity of those victory peptides to be selected, source of species, cystine linkage quantity, makees
With or toxicity molecule target and for the information of species, gene order and protein sequence.This will be in being hereinafter described in detail.
After having selected one or more applicable target substances (45) as described above, according to one or more target substances
(45) degradation mathematical model, and the overhead of corresponding firsthand information (15) is calculated using firsthand information (15), control
Unit (30) receives the overhead and issues control signal (100) accordingly.And the control signal is passed by wireless telecommunications network
It is defeated and received by target substance administration unit (40).The target substance administration unit (40) of control signal is received according to control news
Number (100) select one or more target substances (45) of predetermined concentration and predetermined amount in monitoring region (10).
In one embodiment, after having selected one or more applicable target substances (45) as described above, computing unit
(65) the degradation mathematical model and firsthand information (15) according to target substance (45) summarize corresponding firsthand information (15)
Overhead.
Specifically, wireless telecommunications network include bluetooth, WiFi, radio, radio frequency identification (RFID), ultrasonic,
The wireless communication techniques such as infrared ray, electromagnetic induction.Cloud (50) includes various commercializations, non-commercialization and/or independently developed
Clouds administrative center, cloud data bank or cloud server, such as the MediaTek that Lian Fake (MediaTek) is developed
The cloud data platform of Cloud Sandbox, but not limited to this.
Transmission unit (25,35,35 ') establishes Internet of Things in combination with any intermediary's interface or platform.For example, platform can be used
The IoT platform " IoTtalk " that gulf university of communications is researched and developed is as entire intelligent agriculture and environmental management system the object of framework
The connection bridge of networking.In a preferred embodiment, through IoTtalk, intelligent agricultural of the invention and environmental management system
(1) numerical value that sensor etc. is detected is sent to the IoTtalk platform set up by monitoring unit (20), and as in
Jie's interface, then MediaTek Cloud Sandbox is transferred data to, to establish the data bank on line, and further use this
Data bank carries out data analysis, to assess or monitor the state of region (10).When internet of things equipment is connected to intelligent agricultural
And when environmental management system (1), for various sensors, IoTtalk can be automatically generated or be handled using application software, therefore
Each input equipment can fairly easy be connected to output equipment.However, the invention is not limited thereto, as long as and can establish
Required communication communication, intelligent agricultural of the invention and environmental management system (1) can be used any for establishing Internet of Things
Technology and intermediary's interface.
Intermediary's interface system framework includes network portion and environment division, and wherein network portion is made of an at least system,
Foundation, setting and management system, and it is systematic by internet of things equipment property sort, it manages simultaneously setting input automatically and exports
The connection of function stores all relevent informations in document library system.It executes and communication system is made of two subsystems.Communication
Module system is defined as HTTP based RESTful API (Application Programming Interface), and
Equipment application is supplied to transmit or obtain input-output apparatus information.When internet of things equipment registration/cancel register
(register/deregister) to the execution and when communication system, the equipment application can be required via HTTP API the foundation,
Setting and management system go to change the equipment state in data bank.After internet of things equipment completion is registered to the equipment application, i.e.,
It can be communicated each other via the execution with communication system.It is related defeated to execute connection in sub- module system representative execution network application
The function of entering and export, wherein graphical interface (Graphical User Interface) provide one convenient for users to net
Page interface, can be used to the communication quickly established between line and Internet of Things, via the graphical interface, user can be commanded in this
Jie's interface system framework goes to execute required work, to establish or set device characteristic, function and line setting.
In the case that transmission unit (25,35,35 ') are wire communication network, control unit (30) can be for positioned at cloud
(50) or non-processor positioned at cloud (50) or simultaneously part be located at cloud (50) or part be located at non-cloud processor,
Various data processing platform (DPP)s or the data processing equipments such as calculating center, super computer, data computation library.
In one embodiment, one or more target substances (45) can be selected from composed by chemical substance and biological victory peptide
Group.
In an other embodiments, for example, target substance (45) may include, but are not limited to chemically ingredient or contain
Have the victory insecticide of peptide or protein matter ingredient, nutritional agents, herbicide, growth regulator, acid-base modifier, stain remover, antifreeze,
The various pesticides such as distintegrant, fertilizer, agricultural substance or environment substance.In addition, monitoring region (10) includes field, mountain area, lake
The various scenes such as pool, streams, sandy beach, sea area, wetland, greenhouse, residential quarter, park, athletic ground.
Specifically, target substance (45) includes that there are three cystine linkage and spherical second level knots for tool according to a preferred embodiment
μ-the segestritoxin-sf1a of structure, and monitoring region (10) includes the area invaded and harassed by the pest of Diptera and Lepidoptera
Domain.In the case, the μ-segestritoxin-sf1a of selection can make the pest in region slow down action or paralysis and reduce
Crop is gnawed, and pest may finally be made to cause death because of starvation.According to another embodiment, target substance (45) can be for class
It is similar to the cystine linkage of μ-segestritoxin-sf1a and the ω-hexatoxin-Hv1a (Hv1a) of spherical secondary structure.It is above-mentioned
It is only explanation for target substance (45) and the citing for monitoring region (10), and in the case where meeting intention of the invention, target substance
(45) and region (10) are not limited to above-mentioned example.
According to the type of selected target substance (45), control unit (30) then capable of emitting control signal (100), with
Target substance administration unit (40) are made to select selected target substance (45) in monitoring region (10).For further,
When selecting one or more target substances (45) is chemical substance, computing unit (65) is using by using at least one weather
The degradation rate model of the chemical substance that data is derived is calculated；And one or more target substances (45) are made a living when selection
When physical property wins peptide, computing unit (65) is using the degradation speed by the biological victory peptide at least ultraviolet ray intensity being used to be derived
Rate model is calculated.Then, computing unit (30) can be according to the degradation shape of one or more target substances (45) calculated
State will make target substance administration unit (40) be applied to the pre- of the target substance (45) in monitoring region (10) to adjust and control
Fixed number amount and predetermined concentration.Therefore, it may make that target substance (45) maintains to make for targeted insect in the monitoring region (10)
With or the degree poisoned, and reduce possible excessive accumulation.
In one embodiment, according to the type of selected target substance (45), the capable of emitting control news of control unit (30)
Number (100), so that target substance administration unit (40) applies selected target substance (45) in monitoring region (10).Again into
For one step, when one or more selected target substances (45) are chemical substance, control unit (30) also be can be used at least
The degradation rate model of the chemical substance that one climatological data is derived is calculated；And work as one or more selected objects
When matter (45) is biological victory peptide, the degradation speed for the biological victory peptide that control unit (30) also ultraviolet ray intensity can be used to be derived
Rate model is calculated.Then, control unit (30) also can be according to the degradation of one or more target substances (45) calculated
State will make target substance administration unit (40) be applied to the target substance (45) in monitoring region (10) to adjust and control
Predetermined quantity and predetermined concentration.Therefore, it may make that target substance (45) maintenance can be directed to targeted insect in monitoring region (10)
(45) degree for acting on or poisoning, and reduce possible excessive accumulation.
In one embodiment, target substance administration unit (40) includes solid-state, liquid or gaseous state feedway, and is being received
After the control signal (100) of a specified predetermined amount, with solid-state, liquid or gaseous state supply mode by predetermined concentration or the mesh of predetermined amount
Substance is marked to select into monitoring region (10).However, the invention is not limited thereto, and target substance administration unit (40) include can be by
Target substance (45) are selected to the various devices into monitoring region (10) by various modes.
For example, target substance administration unit (40) includes banishing or flusher, and for example can be by the side banished
Formula discharges the nutritional agents comprising target substance (45) into circulation irrigation canal, with other irrigation conduit or mode point
Cloth is into target monitoring region (10).However, the invention is not limited thereto, and banish or flusher includes being applicable in solid-state, gaseous state
Or the device of liquid.
From the above, as long as can detect required firsthand information (15) or release target substance (45) in monitoring region (10)
In, monitoring unit (20) and target substance administration unit (40) can be located in monitoring region (10) or be located at monitoring region (10) it
Outside.
In one embodiment, computing unit (65) is the framework and operation using tactful algorithm and mathematical model of degrading.
Target substance data bank used in tactful algorithm can be one or more known target substance data bank, or be this hair of arranging in pairs or groups
The target substance data bank that the computing unit (65) of a bright embodiment is established.When target substance data bank collocation computing unit
(65) when establishing, it can be collected by crawler (Crawler) step and log in various victory peptides to be selected in the target substance data bank.In detail
For thin, toxicity is collected on the paper that can be delivered from various protein data bank and for the research achievement of group, toxicity
Or effect wins the data of peptide to establish data bank.
In one embodiment, control unit (30) is the framework and operation using tactful algorithm and mathematical model of degrading.
Target substance data bank used in tactful algorithm can be one or more known target substance data bank, or be this hair of arranging in pairs or groups
The target substance data bank that the control unit (30) of a bright embodiment is established.When target substance data bank collocation control unit
(30) when establishing, it can be collected by crawler (Crawler) step and log in various victory peptides to be selected in the target substance data bank.In detail
For thin, toxicity is collected on the paper that can be delivered from various protein data bank and for the research achievement of group, toxicity
Or effect wins the data of peptide to establish data bank.
For example, an embodiment according to the present invention, when target is can have oral poison to twill burglar moth (Lepidoptera)
Property toxin when, " insecticidal NOT crystal " can be searched on UniProtKB/Swiss-Prot (containing " killing
Worm ", but do not contain " crystallization "), it is all containing insecticidal activity to find out, but do not include the protein of the crystalline protein of Su Li bacterium.
From the above, according to resulting 216 protein, the information for logging in 11 protein is recorded in the object from UniProt
In matter data bank, including protein name, function, species, Amino acid sequence, Amino acid length, cystine linkage quantity, former victory peptide
(pro-peptide), message victory peptide, main chain, UniProt entry number and Arachnoserver number, and object is established with this
Matter data bank.
Specifically, the protein name, function, species, Amino acid sequence, Amino acid length, cystine linkage quantity are
The basic document of protein, and function is the narration of protein function, species are the source species of Amino acid sequence.Original victory peptide and
Message victory peptide represents the sequence that Amino acid sequence can be cut off when the end N- is with the presence or absence of mature or activation.If protein contains former victory
Peptide or message win peptide, then need in addition to collect complete Amino acid sequence and its mature Amino acid sequence (referred to as main chain).Here,
UniProt entry number is accession number of the protein in UniProtKB, and Arachnoserver ID is then another spider toxin
The accession number of data bank ArachnoServer.For further, 7 can be collected from Arachno Server about egg
The data of white matter toxicity: molecular targets are directed to species, effective dose 50, median lethal dose, half paralysis amount, qualitative description, egg
White matter sequence is to help reference.Wherein, molecular targets are the active position of poison victory peptide, such as voltage gated ion channel, inhibition
Neurotransmitter (such as: GABA) receiver etc..For species, effective dose 50, median lethal dose, half paralysis amount, qualitative
Description is then from the result of experiment.
From the above, by crawler step, the target substance data bank used in computing unit (65) can be established.
From the above, by crawler step, the target substance data bank used in control unit (30) can be established.
However, above-mentioned crawler step is merely illustrative, as long as and in base-pair quantity, the species at least recording victory peptide to be selected
Source, cystine linkage quantity, effect or toxicity molecule target and for species, gene order and protein sequence information and meet
Under intention of the invention, crawler step can be set various parameters by various modes to carry out.In addition, direct in computing unit (65)
Using under one or more known target substance data bank, computing unit (65) can implement crawler step first also to extract mesh to be selected out
It marks substance (45).
In one embodiment, computing unit (65) can be based on target substance data bank or based on the object to be selected extracted out
Matter is filtered step and screening step.Specifically, the computing unit (65) of an embodiment according to the present invention uses plan
Slightly algorithm, it includes the following steps for being suitable for biological victory peptide: filtration step are searched, from above-mentioned target substance data bank or
Preset quantity base-pair is searched while had based on the target substance to be selected extracted out, default source of species, is greater than or equal to
The victory peptide of default cystine linkage quantity, then sequentially contain non-spinal, molecule mesh with molecular targets and for species from search result
It marks and is directed to targeted insect for species, and the condition for being less than the victory peptide of predetermined cystine linkage quantity is filtered；And selection step
Suddenly, using protein basis part pairing search tools (such as Protein BLAST) and multiplex paring tool (such as
COBALT) come to be grouped the victory peptide obtained from filtration step according to similar sequences, victory peptide quantity is included in self-grouping result
Apokoinou construction domain is looked in one or more most preceding groups, and selecting the tool in one or more preceding groups according to apokoinou construction domain should
The representative domestic animal peptide in apokoinou construction domain is as target substance.Here, the preset quantity base-pair set in filtration step can be based on
Effect or toxicity are directed to the specific function structural domain of targeted insect and select.
For example, there are about 90% spider venom victory peptides to contain repressor cysteine ??acid structural motif (Inhibitor
Cystine Knot, ICK) structure, this structure be also the main voltage gated ion channel for targeted insect and it is some its
The position of his receiver effect.Therefore, the specific function structural domain of targeted insect can be the knot of repressor cysteine ??acid structural motif
Structure, and the preset quantity base-pair set in filtration step can be selected based on ICK structure.
From the above, the molecular weight of ICK structure and contains at least three cystine linkage 1 between 10kDa.Therefore, root
According to one embodiment of the invention, in order to find institute from 216 protein that the crawler step according to above-described embodiment is established
Biological victory peptide is needed, three screening conditions can be set in the filtration step: preset quantity base-pair presets source of species, is pre-
If cystine linkage quantity；Wherein, preset quantity base-pair is set as 27 to 271 base-pairs (protein of 1kDa averagely contains 9 amine
Base acid, that is, 27 base-pairs), default source of species be set as spider or tarantula, default cystine linkage quantity setting be greater than or
Equal to 3, or for less than 4.Finally, can screen to obtain 113 victory peptides according to this.
In one embodiment, it in order to further be screened from above-mentioned 113 victory peptide, sequentially carries out: containing by molecular targets
" non-spinal " is screened, but retains the victory peptide (63 victory peptides of result) of no corresponding data；Due to according to this embodiment
Targeted insect be prodenia litura, remaining victory peptide after self-sizing (share 14 kinds it is different for species, wherein containing 4 kinds
The insect of Lepidoptera) retrieval for species include at least one Spodoptera litura, Heliothis virescens,
Manduca sexta and Spodoptera exigua, but retain the victory peptide of not data.Come again, considers artificial volume production victory peptide
It is restricted, set the upper limit of predetermined cystine linkage quantity.For example, artificial volume production may produce victory peptide using E.coli, and
E.coli (such as: E.coli Rosetta-gami kind) generally it is difficult to show the protein containing cystine linkage.Therefore, in order to
Ensure that selected victory peptide can set predetermined cystine linkage quantity to filter out excessive cystine linkage less than 4 by manually manufacturing
Win peptide.From the above, 46 victory peptides can be obtained, and the molecular targets of these victory peptides are all that voltage gated ion channel (does not include
There is no data).
In one embodiment, in last selection step, using these victory of the on-line analysis tool for processing of NCBI
Peptide.Specifically, can first using protein basis part pairing search tools (Protein BLAST), find out respectively with this 46
The similar Amino acid sequence of a victory peptide, and be according to this grouped 46 victory peptides.Then, it is looked for using multiplex paring tool (COBALT)
Similarity relation in group between group out, and will not divide the group merging at one group in previous step because of the factors such as branch.
Finally, 46 victory peptides have been divided into 4 groups, there are 27,12,3,2 victory peptides and 2 individual victory peptides respectively.Complete the above process
Afterwards, then with structural domain search, the structural domain that maximum 3 groups share respectively is found out.Such as second largest group, it can be found that it is all
Belong to Toxin_28Superfamily (cl06928).Wherein, the word string in bracket is in protein domain family data bank
Number.Finally, selecting the representative victory peptide of these structural domain families to verify design in this 46 victory peptides.According to this hair
This bright exemplary embodiment, representativeness victory peptide is Hv1a, Sf1a, OAIP.
From the above, in a preferred embodiment, the ortho states egg with multiple cystine linkages and spherical secondary structure can be used
White matter is as target substance (45).For example, ω-hexatoxin-Hv1a (Hv1a) or μ-segestritoxin- can be used
The biological pest repellant of Sf1a is as target substance (45).Wherein, ω-hexatoxin-Hv1a (Hv1a) and μ-
Segestritoxin-Sf1a includes the stabilization ball-type structural domain for having multiple cystine linkages, repressor cysteine ??acid structural motif
(ICK), therefore higher stability is made it have.The process of above-mentioned implementation crawler step, filtration step and selection step is only to show
Example, and in the case where meeting intention of the invention, crawler step, filtration step and selection step according to demand and can require setup parameter
And content.In addition, target substance data bank also may include chemical substance, and select the mistake of chemical substance and biological victory peptide
Journey can respectively or integration carries out.
For further, according to above-mentioned firsthand information (15) also may include the types of one or more targeted insects, quantity,
The targeted insect data of age number can correspondingly select the effective target substance of the different times (age number) of corresponding same targeted insect
(45).For example, can be according to the excessively high target substance for selecting the larva for targeted insect of larva number for the targeted insect being collected into
(45) etc..
From the above, when target substance (45) be chemical substance when, can based on the chemical substance relative to temperature,
Degradation mathematical model that the degradation relationship of an at least climatological data such as humidity, precipitation is derived concludes overhead.In detail and
Speech can carry out mathematicization for the relationship that climatological data is reacted for it, via machine for the chemical substance of every kind of login
Device study, artificial intelligence and economics odds model go to predict and adjust to apply its degradation model.In addition, in order to predict to be similar to
ω-hexatoxin-Hv1a (Hv1a) or μ-segestritoxin-Sf1a, and the ortho states albumen with stable protein structure
The selection opportunity of matter or time interval, computing unit (65) can be used biological victory peptide (protein) relative to ultraviolet ray intensity
Degradation mathematical model that degradation relationship is derived concludes overhead.In the feelings that one or more target substances are biological victory peptide
Under condition, in order to establish the mathematical model based on the degradation rate of protein, the possible degradation way of protein must be studied first
Diameter.Wherein, the possible degradation pathway of protein is respectively that hydrolysis, proteasome degradation and ultraviolet radiation decompose.
In one embodiment, since the anthelminthic effect of the biological pest repellant of such ortho states protein is from its three-level knot
Structure is also considered as and is degraded if being denaturalized linear protein, therefore the rate for being denaturalized linear protein must also be considered in mathematical model
In.Individually below according to Fig. 2 to Fig. 9, for the further details of analytic explanation of these four approach.
According to fig. 2 and the analysis of the hydrolysis pathway of Fig. 3, protein can become the Amino acid of composition again via hydrolysis.By
It is that the E2 that reaction mechanism interrupts peptide bond for the carbon of nucleophilic moieties attack amide groups reacts in hydrolysis, reaction rate is by nucleophilic
The concentration of molecule and protein determines.
The reaction rate equation 1 of hydrolysis pathway is as follows:
Reaction rate equation 1:Wherein, [P] is
Win the concentration of peptide or protein matter, KhFor the reaction rate constant of hydrolysis, kA、kN、kBTo be related with pH-value, for determining KhGinseng
Number.
For the biological pest repellant of this proteinoid, inventor in practice when field because its where environment
PH-value change less, therefore can be by KhIt is considered as a fixed constant.Therefore, the degradation rate of hydrolysis is proportional to egg
The trend of exponential damping can be presented in the concentration of white matter, protein concentration.
In order to test the degradation property of hydrolysis, inventor tests ball-type (ortho states) and linear Hv1a in 4 DEG C of neutrality
The hydrolytic stability of PBS (Phosphate Buffered Saline, pH=7.2-7.5).When Hv1a passes through in 4 DEG C of neutrality PBS
After 1 day and 7 days, inventor is with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, sodium dodecyl
Sulfate polyacrylamide gel electrophoresis) and the remaining protein concentration of ImageJ software calculating.
Fig. 2 and Fig. 3 shows correlated results, and wherein Fig. 2 is the dodecane of Hv1a (protein molecular weight 5.3kDa) and protein labeling
Base sodium sulphate polyacrylamide gel electrophoresis figure, and Fig. 3 is Hv1a- agglutinin (HL, protein molecular weight 17.1kDa) and egg
The sodium dodecyl sulfate polyacrylamide gel electrophoresis figure of white matter label.Wherein, " agglutinin " is snowdrop lectin
(Snowdrop lectin), and Hv1a- agglutinin represents the fusion protein of Hv1a and snowdrop lectin.In some embodiments
In, snowdrop lectin can be used for that biological pest repellant is assisted orally to be transferred to insect enteric epithelium, then pass through intestinal wall
Into the hemolymph of insect.Find that the concentration of ball-type Hv1a changes not behind one day and seven days by the experimental result of Fig. 2 and Fig. 3
(one day: 98%, seven days: 87%), and linear Hv1a was only left 9% after one day, has nearly all degraded after seven days greatly.
Hv1a- agglutinin also has similar result.Ball-type Hv1a- agglutinin will not all be degraded (one day: 110%, seven in seven days
It: 105%), but being only left 3% after linearly Hv1a- agglutinin is left 16%, seven days after one day.It can be pushed away from experimental result
It is disconnected go out, ball-type protein (ortho states protein) in 4 DEG C of neutrality PBS, can't be hydrolyzed in seven days or hydrolysis rate very
Slowly, but linear protein can rapidly degrade with the time.
Protease is inquired into according to fig. 4 to fig. 6 and table 1 for the degradation rate of target victory peptide or protein matter, inventor is
The degradation rate generated by protease is simulated with michaelis-Menton kinetics (Michaelis-Menten kinetics).Due to egg
The reaction rate of white enzyme degradation pathway depend on rate of the protease in conjunction with active position, and proteasome degradation in vitro because
For denatured protein can rapidly be degraded and can be considered to be irreversible.
The reaction rate equation 2 of proteasome degradation approach is (considering rate-limiting step) as follows: equation 2:Wherein, [P] is the concentration for winning peptide or protein matter, Vm,pIt is ferment for the maximum rate of proteasome degradation
Plain total concentration and albumen phosphatase KcatProduct need to be acquired from experimental result depending on different by matter.KM,pIt is normal for Michaelis
Number also represents and works as reaction rate as Vm,pWhen half by matter concentration.It is usually all activated state and holding based on external protease
Balance, it will be assumed that Vm,pFor constant, the equivalent maximum reaction rate of all protease in Lai Daibiao environment.
In the degradation properties of test proteins enzyme, inventor design two experiments with test biological pest repellant Hv1a and
Resistance of the Hv1a- agglutinin to protease.First experiment is ball-type protein and linear protein under the action of protease,
One day interior palliating degradation degree (Fig. 4 and Fig. 5)；Another experiment be linear protein in 4 hours degradation rate (Fig. 6 and
Table 1).
In first experiment, protein is dissolved in neutral PBS (pH=7.2-7.5), and adds serine protease
(trypsase) reacts one day in 37 DEG C.Above-mentioned test result is shown in Fig. 4 and Fig. 5, wherein Fig. 4 is to make through trypsase
After one day, the sodium dodecyl sulfate polyacrylamide gel of Hv1a (protein molecular weight 5.3kDa) and protein labeling
Electrophoretogram, and Fig. 5 is Hv1a- agglutinin (HL, protein molecular weight 17.1kDa) and egg after trypsin acting one day
The sodium dodecyl sulfate polyacrylamide gel electrophoresis figure of white matter label.Above-mentioned experimental result shows, two kinds of ball-type protein
The resistance (111% and 100%) to trypsase is all shown, and linear protein then can be (remaining by trypsin degradation
67% and 18%).And in second experiment, inventor measured in 4 hours, the degradation rate of linear protein, and with
Lineweaver-Burk plot (also referred to as double reciprocal plot) acquires the V of linear Hv1a and linear Hv1a- agglutininm,pWith
KM,p.The above results are shown in Fig. 6 and following table 1.
Table 1: the V of linear Hv1a and linear Hv1a- agglutininm,pAnd KM,p
| Hv1a (no agglutinin) | Hv1a- agglutinin | |
| Vm,p(μg/ml min) | 39.22 | 16.50 |
| KM,p(μg/ml) | 927.29 | 384.47 |
From the above, the degradation rate by illumination institute decomposing protein will be inquired into referring to Fig. 7 A to Fig. 9.Solvent is (usually
For water) by the ultraviolet light in illumination after, the free radical molecule of high activity can be generated, these free radical molecules through propagation
Process can attack protein and interrupt covalent bond before terminating, and make protein degradation.
Actually more complicated radical reaction mechanism is simplified to have with the unknown power n of number of free radical by inventor
It closes, can derive rate equation 3 and equation 4 that the ultraviolet radiation of protein decomposes, and as follows:
Equation 3:
Equation 4:
In upper formulation 3 and 4, wherein [radical] represents the total concentration of free radical, and [P] represents victory peptide or protein
Matter concentration, G γ represent solvent within the unit time, and the radiation energy that per unit mass is absorbed (is 1.42Gy/ for water
S), A γ represents the every free radical quantity (being 0.045 μm of ol/J for water) that absorbs a unit energy and can produce of solvent, I
The intensity of ultraviolet light UVB in sunlight is represented, can be measured from by ultraviolet ray intensity sensor (UVM30A) or other sensors,
RTRepresent the rate constant of radical termination reaction, about 2.365 × 10-7mol-1·s-1, KUVRepresent ultraviolet radiation decomposition
Rate constant, can be acquired by experimental result, simulation when inventor use 44mol-1·s-1It is analyzed.
Above-mentioned equation 3 and 4 is merely illustrative, and in the case where meeting intention of the invention, further includes any subsequent correlation and repairs
Positve term predicts correct degradation rate to increase, wherein the correction term is according to phase produced by an above-mentioned at least climatological data
It closes parameter and carries out the related amendment or convergence of degradation rate accuracy.
Since the solvent in natural environment is generally water, therefore next simulation and experiment all use water as solvent.According to
According to aforesaid equation, inventor calculates the degradation rate that software simulation ultraviolet radiation decomposes using MATLAB.Such as Fig. 7 A to figure
Shown in 7F, degradation rate is also all the more quick with the increase of n, and is eventually completely degraded, the mathematical model derived
Meet the situation used in nature.
In order to further derive equation 3 and equation 4, inventor is further by four kinds of different biological pest repellants
The ball-type protein of (Hv1a, Sf1a, OAIP and Hv1a- agglutinin), being placed in ultraviolet light transmission instrument, (UVB, experiment condition are wave
Long 302nm and intensity 50mW/m2) under irradiate 2 hours.
OAIP is Orally active insecticide peptide (the Orally Active of the toxin from Australian tarantula
Insecticidal Peptide) abbreviation.Experimental result in fig. 8 shows that the situation of four kinds of protein degradations all meets
Above-mentioned derivation model.In the above results, the protein without containing agglutinin and containing agglutinin has very on degradation rate
Apparent difference.For this as a result, a kind of possible reason is that longer protein has higher probability to be attacked by free radical, because
And there is faster degradation rate.Inventor matches experimental result with model (equation 4), and then obtains normal in model
Number n is about 0.78.The K of Hv1a, Sf1a, OAIP and Hv1a- agglutininUVIt is then respectively 2.4,7.8,15.7 and 90.3.In order to test
Demonstrate,prove above-mentioned derivation, inventor Hv1a- agglutinin is placed in varying strength ultraviolet light transmission instrument (UVB, wavelength be 286nm and
Intensity is 36.4mW/m2) tested, and the simulation of degradation rate is carried out with the above results, then carry out with actual experiment result
Compare, comparison result is shown in Fig. 9, it can be seen that deriving model can be predicted the degradation situation of protein.
Inventor carries out for three kinds of hydrolysis, protease and ultraviolet radiation etc. the Effect paths for protein degradation
It inquires into, and mathematical model used in control unit (30) is established with this.The actual degradation rate of protein should be three kinds of ways
The summation of diameter adds the rate R for being transformed into linear proteinSS.Degradation rate equation 5 is as follows after arranging:
Equation 5:
According to degradation rate equation 5, after experiment test, since ball-type protein has hydrolysis, protease
Very high resistance, therefore KhAnd Vm,pCompared to KUVIt is much smaller.From the above, the degradation rate that substantially hydrolyzes and protease
Degradation rate is all negligible compared to the degradation rate of ultraviolet radiation.In addition, because the cystine linkage of protein provides
Very high stability, so being transformed into the rate R of linear protein in the NatureSSThe drop decomposed compared to ultraviolet radiation
Solution rate also can be neglected.
Therefore, degradation rate equation 5 can be further simplified into above-mentioned equation 4, and can allow above-mentioned ultraviolet light
The free radical correlate equation 3 of RADIATION DECOMPOSITION is derived:
Equation 3:
Equation 4:
Wherein, KUVThe ultraviolet radiation decomposition rate constant for corresponding to target victory peptide is represented, [radical] is represented freely
Base total concentration, the victory peptide concentration for the target victory peptide that [P] is once selected before representing, G γ represent the solvent of target victory peptide in unit
The radiation energy that interior per unit mass is absorbed, A γ represent free radical quantity caused by solvent one unit energy of every absorption,
I represents UVB ultraviolet ray intensity, RTThe rate constant of radical termination reaction is represented, n=0.6-0.9, t represent degradation time.Cause
This can carry out the degradation of accurate simulation victory peptide according to the mathematical model of aforesaid equation 3 and equation 4 according to ultraviolet ray intensity
Rate.
Monitoring unit (20), control unit are linked up via Internet of Things according to intelligent agricultural and environmental management system (1)
(30), computing unit (65), transmission unit (25,35,35 ') and target substance administration unit (40), and according to above-mentioned various
Mathematical model assesses the opportunity for needing application target substance (45), number, reason, type or time interval.In one embodiment,
Firsthand information (15) includes ultraviolet ray intensity, especially UVB ultraviolet ray intensity.In the case, monitoring unit (20) detecting sense
After knowing ultraviolet ray intensity, the firsthand information (15) of ultraviolet ray intensity is sent to computing unit (65).Then, computing unit (65)
Mathematical model is applied according to ultraviolet ray intensity, to assess using biological victory peptide as the palliating degradation degree of target substance (45), Jin Er
Order target substance administration unit (40) application target substance (45) when target substance (45) lacks or will lack.
The framework and operation of above-mentioned firsthand information used according to a preferred embodiment of the present invention and mathematical model are only
For example, the invention is not limited thereto.Specifically, in addition to above-mentioned firsthand information (15) and the palliating degradation degree of target substance (45)
Mathematical model outside, the firsthand information (15) of of the invention intelligent agricultural and environmental management system (1) can be single with mathematical model
Solely or further includes other parameters and derive equation.
Under Figure 10 display basis includes number of samples of one or more targeted insects in firsthand information (15), monitoring unit
(20) may include or can be individually for targeted insect count sensing device (2000).Targeted insect counts sensing device (2000) and can wrap
Insect-catching container is included, it includes the cylinders (210) being set on barrel (234) with one or more sluice gates (211), one end connection lock
The channel (220) and accommodating device (230,230 ') of mouth (211) and other end connection accommodating device (230).Cylinder
(210) can be used for that entire targeted insect is protected to count sensing device (2000), and targeted insect can enter mesh by sluice gate (211)
It marks in insect counts sensing device (2000).In addition, in a preferred embodiment, six sluice gates of rectangular hole or trapezoidal hole
(211) it can be respectively arranged below six faces of hexangle type cylinder (210), so that targeted insect can enter, and compared to being set to
The case where top, is less susceptible to pour into rainwater when set on sluice gate (211) Yu Jiangyu of lower section.Here, can type shape, opening is big
Small, quantity and position are merely illustrative, and the invention is not limited thereto.
The accommodating device (230,230 ') that above-mentioned targeted insect counts sensing device (2000) may include insect attraction light source
(231), at least one of targeted insect attractant (232) and pheromones GCMS computer activation neuropeptide (PBAN) (233).
Specifically, coloured light caused by insect attraction light source (231), targeted insect attractant (232) and by targeted insect intake take Lip river
Covering pheromones synthesized by GCMS computer activation neuropeptide (233) has the ability for attracting targeted insect.
For example, the lamp bar that insect attraction light source (231) can be welded by commercially available blue light emitting diode (LED), and
It is attached on accommodating device (230 or 230 ') with hot melt adhesive, such as on acryl cabinet.It is blue light hair in insect attraction light source (231)
In the case where near-infrafed photodiodes (LED), can the targeted insect that be attracted by blue light, such as twill burglar moth, can be easier to be attracted and
Into in accommodating device (230 and 230 ').Like in addition, targeted insect can in addition be arranged in accommodating device (230 and 230 ')
Food, nutritional agents or odor dispersion agent etc. targeted insect attractant (232) so that targeted insect is attracted and enters and hold
It sets in device (230 and 230 ').Or in addition pheromones GCMS computer activation nerve can be set in accommodating device (230 and 230 ')
Peptide (233).When targeted insect enters in accommodating device (230 and 230 ') and absorbs pheromones GCMS computer activation neuropeptide (233)
When, pheromones GCMS computer activates neuropeptide (233) meeting stimulation target insect and discharges pheromones, and the pheromones discharged can be more into one
Step attracts more targeted insects to enter in accommodating device (230 and 230 ') in specific manner.In the examples described above, accommodating device
(230 and 230 ') can only include insect attraction light source (231), targeted insect attractant (232) or pheromones GCMS computer activation neuropeptide
One of (233), also or accommodating device (230 and 230 ') may include insect attraction light source (231), targeted insect attractant
(232) multiple combination or in pheromones GCMS computer activation neuropeptide (233).
Targeted insect according to an embodiment of the present invention counts sensing device (2000), in another on one-way trip sluice gate (211)
Infrared counter (240) are provided with outside, to calculate the target elder brother passed through when targeted insect passes through one-way trip sluice gate (211)
Borer population amount.For example, infrared counter (240) may be disposed on one-way trip sluice gate (211) or channel (220).Work as mesh
The infrared ray issued by RF transmitter (241) is truncated by one-way trip sluice gate (211) or channel (220) in mark insect
When, targeted insect, which counts sensing device (2000), will record the number that infrared receiver (242) does not receive infrared ray, to
Calculate the number of samples of targeted insect.
In one embodiment, may include two groups of infrared detectors, and when two groups of infrared detectors infrared ray by according to
When sequence is truncated, situations such as just will record the number of samples for targeted insect, flutter its wings up and down or hover in the channel to avoid targeted insect
Caused by error count.In this, infrared counter is merely illustrative, as long as the number of samples of targeted insect can be detected, counter can
For various devices.
Other than above-mentioned apparatus, targeted insect, which counts sensing device (2000), can further include signal processor and charge
Storage device (250), signal processor and charge storage device (250) can provide targeted insect and count sensing device (2000) institute
The firsthand information (15) that the energy and/or preliminary treatment and transmission needed is counted from infrared counter (240) is single to calculating
First (65).
For example, signal processor and charge storage device (250) they can be Arduino Mega2560 development board, and
Firsthand information (15) are reached Linkit Smart 7688Duo exploitation through I2C mode by 2560 development board of Arduino Mega
Plate carries out cloud and is uploaded to control unit or other devices (for example, cell phone application) to reach monitoring purpose.Wherein, Linkit
Smart 7688Duo development board can have MPU and MCU (such as: Lian Fake chip and Arduino chip), and MCU receive it is original
Data (15) is transmitted to MPU again, and MPU carries out calculation process into required information.For further, for example, MCU can be
ATmega32U4, and MPU can be MT7688AN.However, the invention is not limited thereto, as long as and can carry out appointing for above-mentioned micro-control function
What route, chip, circuit, device etc. is all used as signal processor and charge storage device (250).For example, can be with
MCP23008/MCP23S17IC carries out I/O expansion and is aided with NE555 to replace 2560 development board of Arduino Mega.
In one embodiment, accommodating device (230 and 230 ') may include barrel (234) and be surrounded by barrel (234)
Accommodating space, and the accommodating space is separated into back taper space and trap space by tilting Partition device.Wherein, the back taper space
Upper part have communicating passage one or more entrances, and the lower part in the back taper space have towards trap space connection
Mouthful.In the case, the back taper space for being designed as inverted pyramid type enables targeted insect to facilitate in the trap space of entrance lower section
But it is not easy to escape from, and then achievees the purpose that trapping.Here, the entrance in back taper space and the ratio of connector are to say for convenience
For the sake of bright and outline is painted, and its size and relative scale can non-be as shown in the figure.For example, in a preferred embodiment, connection
Mouth can be far smaller than entrance.For example, the size of connector and entrance can be 1:2,1:3,1:4,1:5,1:10,1:
12,1:15 or 1:20 etc., and it is without being limited thereto.
According to above-described embodiment, targeted insect attractant (232) and pheromones GCMS computer activation neuropeptide (233) can be with
It is seated in trap space.However, according to a preferred embodiment of the present invention, targeted insect attractant (232) and pheromones
GCMS computer activation neuropeptide (233) may be disposed in back taper space, to attract targeted insect more easily.It for example, can be
Hole plug is sucted the bait of liquid with capillary phenomenon, or can be in connector with cotton or the fiber substance of toilet paper
Support plate or partition is arranged in part range, and targeted insect attractant (232) and pheromones GCMS computer are activated neuropeptide
(233) it is placed on support plate or partition.However, above are only example, as long as and targeted insect can be attracted, targeted insect is lured
Agent (232) and pheromones GCMS computer activation neuropeptide (233) can be set in above-mentioned any structure according to demand, and unlimited
In the embodiment specifically listed at this.
In addition, above-mentioned design can also be separated into different interlayer spaces respectively.For example, it by back taper space and can fall into respectively
Trap space is separated into more than one interlayer space, and each interlayer space places the pheromones bait or bubble for being directed to different target insect
Pheromones GCMS computer activation neuropeptide (233) opened, and is arranged hole, plug with cotton or the fiber substance of toilet paper, with
The bait of liquid is sucted and is eaten for targeted insect by capillary phenomenon, and then attracts different specific objective insects.
Under the design in above-mentioned back taper space and trap space, in addition can arrange in pairs or groups channel (220), " bow " such as printd with 3D
Font body or labyrinth passage, it is logical by difference to calculate to be put into RF transmitter (241) and infrared receiver (242)
Road (220) enters the targeted insect quantity of different interlayer spaces.
Here, targeted insect counts the cylinder (210) of sensing device (2000), barrel (234), for being separated out back taper sky
Between and trap space inclination Partition device, for separate be directed to different target insect interlayer space spacing body and/or other
Space setting, which separates appliance, to print material etc. for acryl, glass, stainless steel, metal, alloy or 3D.However, the present invention is not
It is limited to this.And in the case where meeting intention of the invention, cylinder (210), barrel (234), inclination Partition device, spacing body and/or other
It can be a variety of materials and size that space setting, which separates appliance,.
The above-mentioned explanation that sensing device (2000) are counted for targeted insect is all example, and is meeting intention of the invention
Under, targeted insect, which counts the configuration of sensing device (2000) and design, can have various change or can allow other devices or structure
Carry out united application.
Monitoring unit (20) include targeted insect count sensing device (2000) so that firsthand information (15) include one or
In the case where the number of samples of plurality of target insect, control unit (30) can be according further to the sampling of one or more targeted insects
Whether quantity is more than predetermined amount, and issues control signal (100), makes target substance administration unit (40) applying pesticide or expelling parasite
Agent.
Insect nutritional agents etc. is the control substance for decreasing or increasing targeted insect.In the case, control unit (30)
Mathematical model can for one or more targeted insects number of samples and above-mentioned degradation rate distinctly with the whole of different weights measurement
Close mathematical model.However, the invention is not limited thereto, and the mathematical model of computing unit (65) also can be only with one or more targets elder brother
The number of samples of worm or above-mentioned degradation rate are established.
In one embodiment, the mathematical model of control unit (30) can utilize different parameters and one or more targeted insects
Number of samples is expected following targeted insect quantity, and machine selects target substance (45) in advance or in due course.Also that is, calculating
The mathematical model of unit (65) may include targeted insect prediction model.
For example, in one embodiment, target substance (45) may have the targeted insect in particular growth period
Specificity, and do not have specificity to other growth periods of same target insect.Therefore, according to the present invention, can further supervise
The firsthand information during one section in the past is surveyed to predict the targeted insect quantity of specific period.For example, if targeted insect is moth class,
And target victory peptide it is more effective for the larva of moth class, and harm the crops mainly moth class larva when, due to moth class from
Pupa becomes time of moth day to be differed from 6 days to 14, and can set according to this need to monitor over the accumulated numbers of 20 hawkmoths (caught in the 20th day
The moth obtained must be on day 1 larva, however this is merely illustrative, and can also set other numerical value), using the mesh as prediction model
Mark.The larva that this prediction model can select target victory peptide to influence moth class in due course is established, to manage the adult of the following possible moth class
Quantity.
In one embodiment, it may include air pressure in 20 days, average gas pressure, highest gas in the past that inventor, which designs firsthand information,
Temperature, the lowest temperature, temperature on average, highest humidity, minimum humidity, medial humidity, rainfall, average rainfall, moment rainfall,
Wind speed, wind direction, fog distributed degrees, haze distributed degrees, illumination, average time illumination, soil moisture, various gas concentrations etc.
At least number of samples of a data and the targeted insect of accumulation.According to this preferred embodiment, monitoring unit (20) can be further
Sensor or sensing device comprising the various known or following exploitation for detecting above-mentioned firsthand information (15).
In the case, python 3.5 can be used in prediction model, utilizes recurrent neural network (RNN) and artificial neural network
Constructed by Deep Learning frame of the combination of network (ANN) with tensor flow, and trained prediction is corrected with above-mentioned firsthand information
Model.Wherein, 140 features are first to compress by recurrent neural network according to time series to above-mentioned 20 days 7 features in total
At 7 compressive features, the adult quantity and the 10th of 7 compressive features (climatological data) and the 1st day to the 10th day accumulation moth class
It then is input to artificial mind to adult quantity (two features of supplement) totally 9 feature neurons of the 20th day accumulation moth class
Carry out prediction result through network.By the error calculated between practical answer and output, repaired using gradient decline and backpropagation
Change the weight of each neuron in each network.After training, inventor obtains the prediction mould of the accuracy with about 80%
Type.However, the invention is not limited thereto, and the data that sustainable use is collected in region (10) carry out re -training amendment prediction mould
Type, and can remodify and train according to different floor areas to obtain particular prediction model.
Although above-mentioned used air pressure in 20 days, average gas pressure, the highest temperature, the lowest temperature, temperature on average, highest in the past
Humidity, minimum humidity, medial humidity, rainfall, average rainfall, moment rainfall, wind speed, wind direction, fog distributed degrees, mist
At least one data such as haze distributed degrees, illumination, average time illumination, soil moisture, various gas concentrations and the mesh of accumulation
The number of samples of insect is marked to correct artificial intelligence's model of trained neural network, however the invention is not limited thereto.For example,
According to different target insect or the different growing stages specific aim of target victory peptide, instant, past 1 day, past 3 days, past 5 can be used
It, past 10 days, the firsthand information (15) that prediction model is collected during past 15 days or the different time such as 30 days in the past,
And the one or more of combination of air pressure, the highest temperature, the lowest temperature, average air epidemic disaster, precipitation, wind speed can be used to arrange in pairs or groups
Correct training pattern, also or using features described above outside firsthand information (15) carry out correction model.For example, features described above can be used
The varying environment parameter other than climate parameter or climate parameter in addition, is corrected including but not limited to as described above
Training pattern.
Next, 1A referring to Fig.1 to Figure 13 is illustrated intelligent agricultural and environment according to other embodiments of the invention
Management system.In Figure 11 A into Figure 13, illustrate the difference different from Fig. 1, and the details being described in detail in Fig. 1 for main
It will simply describe or omit for the sake of succinct and is clear.
Firstly, 1A, intelligent agricultural and environmental management system (2) further include far-end operation unit (60) referring to Fig.1.
Far-end operation unit (60) receives via wireless telecommunications network (55) from computing unit (65) and shows firsthand information (15), management
The combination of information or firsthand information (15) and overhead, and have operation interface selectively to issue manual control signal
(200) to target substance administration unit (40) with application target substance (45).For example, 1B referring to Fig.1, far-end operation unit
It (60) can be Smartphone, and user can receive firsthand information (15) either through control unit (30) from Smartphone
Or computing unit (65) it is processed after overhead (300).Specifically, Smartphone can directly display comprising temperature,
The firsthand information (15) of wind speed, humidity and targeted insect number of samples etc., and show and be computed unit (65) or control unit
(30) suggest the processing information (300) of user after processing calculates.User is with reference to above-mentioned firsthand information (15), processing information
(300) or both after combination, can decide whether to apply target substance (45) by operation interface (400), and under instruction
Target substance administration unit (40) will be transmitted to by manual control signal (200) to take action.
In one embodiment, according to Figure 12 A, intelligent agriculture and environmental management system (3) monitoring unit can be divided into prison
Unit (20) and main monitoring unit (20 ') are surveyed, and is received in the different blocks of monitoring region (10) from each monitoring unit (20)
The firsthand information (15) collected first can be transmitted to main monitoring unit (20 ') via the transmission network (500) of such as bluetooth.Main monitoring
Unit (20 ') is converging after the whole firsthand information (15) from each monitoring unit (20), then as shown in Figure 1, via transmission unit
(25) computing unit (65) or control unit (30) are transmitted to.
In one embodiment, according to Figure 12 B, the target substance administration unit of intelligent agricultural and environmental management system (4)
Target substance administration unit (40) and major heading substance administration unit (40 ') can be divided into, and single from control unit (30), calculating
The instruction of first (65) or far-end operation unit (60) is first transmitted to major heading substance administration unit (40 '), then via such as bluetooth
Transmission network (600) transmit and control each target substance administration unit (40) in monitoring region (10) in different blocks in
It selects.
In Figure 12 A and Figure 12 B, for convenience of explanation for the sake of, it is single with monitoring in intelligent agricultural and environmental management system
Other first or unrelated target substance administration unit devices will give in omission.In addition, configuration can be with shown in Figure 12 A and Figure 12 B
The intelligent agricultural of above-mentioned other embodiments and the aspect of environmental management system are combined, so as to include field or other places
Region (10) monitoring and control become more accurate.
In one embodiment, Figure 13 is different from intelligent agricultural shown in FIG. 1 and environmental management system (1), intelligent agriculture
The received firsthand information (15) of monitoring unit (20) institute can be also directly transferred to target substance and applied by industry and environmental management system (5)
With unit (40), and target substance administration unit (40) can the built-in mathematical model of direct basis to assess which kind of object selected
Matter (45) and/or whether answer application target substance (45).Alternatively, intelligent agricultural and environmental management system (5) can also monitor
After handling the received firsthand information (15) of institute using mathematical model in advance in unit (20), then transmit orders to target substance to apply single
First (40) make it select simultaneously application target substance (45).
Other than above-mentioned configuration, in the case where meeting intention of the invention, intelligent agricultural and environmental management system can be above-mentioned
The combination of the configuration of different intelligent agriculturals and environmental management system is applied.In addition, intelligent agricultural and environmental management system
Also it can integrate and arrange in pairs or groups other devices or equipment use.For example, the monitoring unit (20) of intelligent agricultural and environmental management system,
Computing unit (65), control unit (30), target substance administration unit (40) and/or far-end operation unit (60) it is one or more
A combination, all capable of emitting feedback signal and the whole information of system to research institution, and research institution can using these feedback signals and
Whole information unite further to study, or even mathematical model or framework used in amendment Internet of Things.
Above revealed collocation mathematical model and the intelligent agricultural and environmental management system of Internet of Things can be used for respectively
Kind purposes.For example, intelligent agricultural and environmental management system can be used for monitoring the quantity of dengue fever vector mosquito in residential quarter,
And the vector mosquito specificity toxicity that can kill or expel dengue fever vector mosquito is selected to win peptide to manage vector mosquito quantity.In another reality
It applies in example, intelligent agricultural and environmental management system can be used and come from pipe network spider (Segestria florentina) (Tube-
Web spider) μ-segestritoxin-Sf1a or artificial recombination albumen with similar structure as biological pesticide,
And then the quantity of the targeted insect of common Diptera (flies) and Lepidoptera (moth class) pest in field is managed in specific manner.It is such
Common pest is, for example, twill burglar moth (Spodoptera litura), beet armyworm (Spodoptera exigua
Hubner), bean-pod borer (Maruca testulalis) etc..In another embodiment, intelligent agricultural and environmental management system can
The attraction victory peptide or chemical substance to beneficial objectives insect tool specificity are disseminated in afield, so that such beneficial objectives insect
Quantity maintain expected degree.
Intelligent agriculture and environmental management system the purposes of above-mentioned explaination is merely illustrative, and is meeting intention of the invention
Under, intelligent agricultural of the invention and environmental management system can be applicable in various scenes and purposes.
From the above, intelligent agricultural and environmental management system according to the present invention, includes by tactful algorithm and degradation
Rate Models are integrated into Internet of things system, and can be in addition further combined with targeted insect prediction model, more accurately to hold
The continuous target substance for selecting predetermined extent, and then reach expected agricultural environment control.By intelligent agricultural and environmental management
System can be established and reduce manpower, more environmentally friendly environmental management system, to reduce by more than the residual of the target substance outside desired amount
Property and select randomness.
It will be understood by those skilled in the art that above embodiments are only exemplary embodiments, without departing substantially from spirit of the invention
In the case where range, a variety of variations can be carried out, replaced and changed.
Claims (20)
1. a kind of intelligent agricultural and environmental management system, which is characterized in that the intelligent agricultural and environmental management system packet
It includes:
Monitoring unit, to collect the firsthand information in monitoring region；
Transmission unit is mutually coupled with the monitoring unit, to receive and transmit the firsthand information；
Computing unit is mutually coupled with the transmission unit, to receive firsthand information and foundation from the transmission unit
Tactful algorithm selects target substance in target substance data bank, and degradation mathematical model and institute according to the target substance
Firsthand information is stated to conclude overhead；
Control unit is mutually coupled with the computing unit, to receive the overhead and generate control signal；And
Target substance administration unit, is connected with described control unit, to receive the control signal, and according to the control
Signal is in the monitoring Zoned application predetermined concentration and the target substance of predetermined amount.
2. intelligent agricultural according to claim 1 and environmental management system, which is characterized in that the monitoring unit includes
Targeted insect counts sensing device, temperature-sensing device, rain gauge, illumination detection device, carbon dioxide sensing device, ultraviolet light
At least one of which of intensity sensing device, soil moisture sensing device and air pressure sensing device.
3. intelligent agricultural according to claim 2 and environmental management system, which is characterized in that the targeted insect counts
Sensing device includes insect-catching container and the insect attraction light source being set in the insect-catching container, targeted insect attractant and infrared ray
Counter.
4. intelligent agricultural according to claim 3 and environmental management system, which is characterized in that the insect-catching container packet
It includes:
Cylinder has barrel and an at least open end, and an at least open end is set to the both ends of the barrel, the cylinder
The first space and second space are divided into tilt Partition device in inside, and first space and second space are connected；
An at least lid is set to an at least open end for the cylinder, and an at least lid, which has, is set to the lid
The fixed part of inside, to the fixation targeted insect attractant；And
An at least one-way trip sluice gate, is set to the barrel, wherein the infrared counter is set to the interior of the cylinder
Portion, and be located on the barrel and adjacent to an at least one-way trip lock.
5. intelligent agricultural according to claim 4 and environmental management system, which is characterized in that the insect-catching container is into one
Step includes signal processor, is set to the inside of the cylinder, and with the infrared counter and the insect attraction light source phase
Coupling.
6. intelligent agricultural according to claim 4 and environmental management system, which is characterized in that the insect-catching container is into one
Step includes charge storage device, is connected with the cylinder, to provide the electric energy of the insect-catching container.
7. intelligent agricultural according to claim 1 and environmental management system, which is characterized in that the target substance is to change
Learn substance, biological victory peptide or combinations thereof.
8. intelligent agricultural according to claim 1 and environmental management system, which is characterized in that the target substance is made a living
Physical property wins peptide, and the degradation mathematical model is generated according to following equation 1 and formula 2, and through ultraviolet ray intensity derivation
The degradation rate model of the biological victory peptide:
Formula 1:
Formula 2:
Wherein, KUVRepresent the ultraviolet radiation decomposition rate constant of the biological victory peptide；It is always dense that [radical] represents free radical
Degree；The concentration of the biological victory peptide of applied once before [P] is represented；G γ represents the solvent of the biological victory peptide in unit
The radiation energy that per unit mass is absorbed in time；A γ is represented caused by the solvent one unit energy of every absorption freely
Radix amount；I represents the ultraviolet ray intensity；RTRepresent the rate constant of radical termination reaction；T represents resolving time and n
=0.6-1.5.
9. intelligent agricultural according to claim 1 and environmental management system, which is characterized in that the computing unit is into one
Step is come according to the firsthand information and via the targeted insect counting resulting targeted insect quantitative forecast model of sensing device
Conclude the overhead.
10. intelligent agricultural according to claim 9 and environmental management system, which is characterized in that the targeted insect number
It measures prediction model system and uses python, and using the combination of recurrent neural network and artificial neural network, with tensor flow's
Constructed by Deep Learning frame, wherein the firsthand information further includes plurality of parameters, and the targeted insect quantity is pre-
Surveying model is that training is corrected using the plurality of parameters and the targeted insect quantity of accumulation, and by recurrence mind
The plurality of parameters is compressed into compressive features respectively according to time series through network.
11. it is according to claim 10 it is intelligent agricultural and environmental management system, which is characterized in that the compressive features and
Supplement setting feature is input to the artificial neural network jointly, modifies the pressure by gradient decline and backpropagation is used
Each weight of contracting feature and the supplement setting feature.
12. intelligent agricultural according to claim 1 and environmental management system, further include far-end operation unit, institute
It states far-end operation unit and shows the firsthand information, described overhead or combinations thereof, and the far-end operation unit has behaviour
Make interface, selectively to issue manual control signal to the target substance administration unit, to apply predetermined concentration and pre-
The quantitative target substance.
13. intelligent agricultural according to claim 8 and environmental management system, which is characterized in that the strategy algorithm
It include to search to be suitable for the step of applying the biological victory peptide, comprising:
Filtration step is searched from the target substance data bank that crawler step is established while having preset quantity base
It with molecular targets and is directed to, default source of species, more than or equal to the victory peptide of default cystine linkage quantity, then from search result
Species contain non-spinal, molecular targets and contain targeted insect for species, and are less than the item of the victory peptide of predetermined cystine linkage quantity
Part is filtered；And
Step is selected, to be grouped according to similar sequences using protein basis part pairing search tools and multiplex paring tool
The victory peptide obtained from the filtration step, comprising looking for apokoinou construction in the most preceding group of victory peptide quantity in self-grouping result
Domain, and selected described in the representative victory peptide conduct in the preceding group with the apokoinou construction domain according to the apokoinou construction domain
Target substance.
14. intelligent agricultural according to claim 12 and environmental management system, which is characterized in that the preset quantity alkali
Base is set as spider or tarantula and the default cystine linkage number to 27 to 271 base-pairs, the default source of species is set as
Amount the first setting value be more than or equal to 3 and second setting value be less than or equal to 4.
15. intelligent agricultural according to claim 12 and environmental management system, which is characterized in that the preset quantity alkali
Base to be based on effect or toxicity for the targeted insect specific function structural domain and set.
16. intelligent agricultural according to claim 15 and environmental management system, which is characterized in that the specific function knot
Structure domain is the structure of repressor cysteine ??acid structural motif.
17. intelligent agricultural according to claim 13 and environmental management system, which is characterized in that the crawler step packet
Various candidate victory peptides are logged in the target substance data bank containing collecting, and record the base logarithm of at least described candidate domestic animal peptide
Amount, source of species, cystine linkage quantity, effect or toxicity molecule target and money for species, gene order and protein sequence
News.
18. intelligent agricultural according to claim 1 and environmental management system, which is characterized in that the target substance packet
- the hexatoxin-Hv1a containing ω or μ-segestritoxin-Sf1a.
19. intelligent agricultural according to claim 3 and environmental management system, which is characterized in that the targeted insect draws
Lure is that insect pheromones GCMS computer activates neuropeptide.
20. intelligent agricultural according to claim 3 and environmental management system, which is characterized in that the insect attraction light source is
Blue-ray LED illuminator.
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