CN109797176A - A kind of environment-protective process preparing monosodium glutamate - Google Patents
A kind of environment-protective process preparing monosodium glutamate Download PDFInfo
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- CN109797176A CN109797176A CN201711141148.7A CN201711141148A CN109797176A CN 109797176 A CN109797176 A CN 109797176A CN 201711141148 A CN201711141148 A CN 201711141148A CN 109797176 A CN109797176 A CN 109797176A
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Abstract
The invention belongs to monosodium glutamate preparation technical fields, disclose a kind of environment-protective process for preparing monosodium glutamate comprising following steps: step 1) prepares glutami acid fermentation liquor, and step 2 bacterial strain recycles, and step 3) prepares monosodium glutamate.The present invention improves the yield of monosodium glutamate by being produced acid again to discarded thallus.
Description
Technical field
The invention belongs to monosodium glutamate preparation technical fields, specifically provide a kind of environment-protective process for preparing monosodium glutamate.
Background technique
Monosodium glutamate, scientific name sodium glutamate, one sodium of chemical name alpha-amido glutaric acid are that one kind is formed by sodium ion and glutamate ion
Salt, Glutamic Acid is a kind of amino acid, and sodium is a kind of metallic element.In life common seasoning monosodium glutamate it is main at
Dividing is exactly sodium glutamate.Monosodium glutamate is common flavoring agent in daily life, can increase the delicate flavour of food, is conducive to improve human body
To the digestibility of food.In addition, sodium glutamate has highly important function again, be widely used in food, medicine, industry and
The fields such as agricultural.
Currently, the main method for preparing monosodium glutamate is using microbial fermentation technology.Bacterial strain by producing glutamic acid is sent out
Ferment, glutamic acid is secreted extracellular, is carried out processing to fermentation liquid and is obtained monosodium glutamate, then extraction purification.Microbial fermentation is to determine taste
The emphasis of principal element and monosodium glutamate the preparation business research of smart yield.In fermentation process, cell membrane is to metabolite
Permeability in fermentation industry be a particularly significant problem.More relatively broad being of permeability of cell membrane is improved at present
Method, the disadvantage is that low efficiency, intracellular organic matter release rate is low, and the processing time is long, and reagent dosage is big, at high cost, post-processes simultaneously
Also inconvenient, and due to the toxicity of chemical reagent, it need to further be removed with the methods of dialysis when separation.
" transformation conversion technology " is to apply temperature by the certain phase in biological respinse according to microorganism self character
And pressure, the new new bio processing method of the one kind for reinforcing cell metabolism flux along purpose product direction.Transformation biology
Conversion changes way of the pressure as constant in traditional microbiological fermentation process.By selecting suitable pressure medium and pressurization
Mode can be such that microbial activity is substantially unaffected.Pressurization can improve solubility property of the indissoluble substrate in water phase, change
The permeability of microbial cell film improves the mass transfer rate of matrix, product, changes microbial metabolism stream, is finally reached raising hair
The purpose of ferment level.Glutamic acid fermentation produces the sour later period, will cause pyruvic acid or lactic acid etc. and largely accumulates, needs to stop sending out at this time
Ferment removes thallus by way of filtering or being centrifuged, and then fermentation liquid is purified to obtain product；However, discarded thallus
Still have certain acid producing ability, how to be again that the technology that we need to solve is asked with sharp high acid amount to discarded thallus
Topic.
Summary of the invention
For overcome the deficiencies in the prior art, acid is produced again to discarded thallus, to improve the yield of monosodium glutamate, the present invention is mentioned
A kind of environment-protective process for preparing monosodium glutamate is supplied.
The present invention is realized by following scheme:
A kind of environment-protective process preparing monosodium glutamate comprising following steps: step 1) prepares glutami acid fermentation liquor, and step 2 bacterial strain is again
It utilizes, step 3) prepares monosodium glutamate.
Further,
The step 1) prepares glutami acid fermentation liquor, include the following steps: by Corynebacterium glutamicum seed liquor according to 8% inoculation
Amount, which is inoculated in the fermentor equipped with fermentation medium, ferments, and 35 DEG C of cultivation temperature, by adjusting speed of agitator and ventilation
It is 20% that amount, which keeps dissolved oxygen level, and pH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by auto-feeding ammonium hydroxide；
And residual sugar is controlled for the glucose solution of 100g/L by stream plus concentration and is being not less than 1.0%, fermentation to 48h stops, and obtains paddy
Propylhomoserin fermentation liquid.
Further,
The component of the fermentation medium are as follows: glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, sulphur
Sour magnesium 0.01%, potassium dihydrogen phosphate 0.01%, pH6.8, the above are mass ratioes.
Further,
The step 2 bacterial strain recycles, and includes the following steps: that glutami acid fermentation liquor through micro-filtrate membrane filtration, collects wet thallus respectively
With filtered solution A；1-2wt% hydrogen peroxide and 2-3wt% tourmaline are added into wet thallus, 100rpm stirs 60min, then stops
Stirring is heated to 50-55 DEG C, keeps the temperature 60-120s, and cooled to room temperature is then added in the Dialysis culture base of double weight,
35 DEG C of cultivation temperature, 100rpm stir culture 6h, micro-filtrate membrane filtration collects thallus and filtered solution B.
Further,
The Dialysis culture base are as follows: potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.02%, magnesium sulfate 0.02%,
Zinc sulfate 0.01%, adjustment pH are 6.8, and the above are mass ratioes.
Further,
The microfiltration membranes are inorganic ceramic membrane, and molecular cut off 2000Da, micro-filtration temperature is 35 DEG C.
Further,
The step 3) prepares monosodium glutamate, includes the following steps: to merge filtered solution A and filtered solution B to obtain mixed liquor A, toward mixing
Addition accounts for the sodium carbonate of 1/10th parts by weight of mixed liquor A in liquid A, and 100rpm stirs 30min, obtains mixed liquid B, then will mix
It closes liquid B to be added in decoloration pump, adds the active carbon that mass fraction is 1%, decolourize 30min；10min is centrifuged with 3000rpm again,
Collect destainer；Destainer is added in concentration pan, in 65 DEG C of progress concentrations, obtains monosodium glutamate crude product.
The beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
The present invention is improved for microbial fermentation technology, is avoided aminoglutaric acid concentration intracellular from running up to and is caused feedback inhibition tune
Section carries out secondary treatment for discarded thallus, increases permeability of cell membranes, improve the acid producing ability of bacterial strain；
The acid producing ability and permeability of cell membrane of bacterial strain can be improved in the heat treatment of appropriate time and temperature, cooperates Dialysis culture
Base, so that the yield of glutamic acid greatly improves；
Tourmaline can discharge anion automatically, and anion has stronger oxidisability, and DC static also persistently occurs, and discharge mine
Substance and microelement, play a driving role to microbial reproduction；By cheap natural minerals tourmaline and hydrogen peroxide
It is mixed with microorganism, discharges oxygen and negative ion element is that microorganism provides breeding and metabolic conditions；
The present invention cultivates bacterial strain using Dialysis culture base, and the adjusting of feedback inhibition caused by glutamic acid accumulation substantially reduces,
It produces sour efficiency to improve, and subsequent residual sugar is few, not will cause bacterial strain, to stick together flocculation agglomerating, has and utilizes the filtering point of subsequent film
From；
Using isolation technics first after fermentation, removal generates the substance of inhibiting effect, since inhibiting effect releases, cellular enzymes
Work is restored, and compared to for common fermentation, the entire fermentation process production sour period is greatly prolonged.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, specifically real below in conjunction with the application
Example is applied, the present invention is more clearly and completely described, it is clear that described embodiment is only that the application a part is real
Example is applied, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making creation
Property labour under the premise of every other embodiment obtained, should fall within the scope of the present invention.
Embodiment 1
A kind of environment-protective process preparing monosodium glutamate comprising following steps:
By Corynebacterium glutamicum seed liquor (1 × 108Cfu/ml) hair equipped with fermentation medium is inoculated according to 8% inoculum concentration
It ferments in fermentation tank, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by certainly
PH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by dynamic Feeding ammonia water；And concentration is added to be the grape of 100g/L by stream
Sugar juice, which controls residual sugar, is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor；Wherein, fermentation medium is
(mass percent): glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, phosphoric acid
Potassium dihydrogen 0.01%, pH6.8；
Glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；1wt% hydrogen peroxide is added into wet thallus
And 2wt% tourmaline, 100rpm stir 60min, then stop stirring, are heated to 55 DEG C, keep the temperature 80s, naturally cool to room
Temperature is then added in the Dialysis culture base of double weight, and 35 DEG C of cultivation temperature, 100rpm stir culture 6h, micro-filtrate membrane filtration is received
Collect thallus and filtered solution B；The Dialysis culture base is (mass percent): potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, sulphur
Sour ferrous iron 0.02%, magnesium sulfate 0.02%, zinc sulfate 0.01%, adjustment pH are 6.8；Above-mentioned microfiltration membranes are inorganic ceramic membrane, retention point
Son amount is 2000Da, and micro-filtration temperature is 35 DEG C；
Merge filtered solution A and filtered solution B to obtain mixed liquor A, into mixed liquor A, addition accounts for 1/10th parts by weight of mixed liquor A
Sodium carbonate, 100rpm stir 30min, obtain mixed liquid B, then by mixed liquid B be added decoloration pump in, add mass fraction
For 1% active carbon, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；Destainer is added in concentration pan,
In 65 DEG C of progress concentrations, monosodium glutamate crude product is obtained.
Embodiment 2
A kind of environment-protective process preparing monosodium glutamate comprising following steps:
By Corynebacterium glutamicum seed liquor (1 × 108Cfu/ml) hair equipped with fermentation medium is inoculated according to 8% inoculum concentration
It ferments in fermentation tank, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by certainly
PH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by dynamic Feeding ammonia water；And concentration is added to be the grape of 100g/L by stream
Sugar juice, which controls residual sugar, is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor；Wherein, fermentation medium is
(mass percent): glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, phosphoric acid
Potassium dihydrogen 0.01%, pH6.8；
Glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；2wt% hydrogen peroxide is added into wet thallus
And 3wt% tourmaline, 100rpm stir 60min, then stop stirring, are heated to 50 DEG C, keep the temperature 120s, naturally cool to room
Temperature is then added in the Dialysis culture base of double weight, and 35 DEG C of cultivation temperature, 100rpm stir culture 6h, micro-filtrate membrane filtration is received
Collect thallus and filtered solution B；The Dialysis culture base is (mass percent): potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, sulphur
Sour ferrous iron 0.02%, magnesium sulfate 0.02%, zinc sulfate 0.01%, adjustment pH are 6.8；Above-mentioned microfiltration membranes are inorganic ceramic membrane, retention point
Son amount is 2000Da, and micro-filtration temperature is 35 DEG C；
Merge filtered solution A and filtered solution B to obtain mixed liquor A, into mixed liquor A, addition accounts for 1/10th parts by weight of mixed liquor A
Sodium carbonate, 100rpm stir 30min, obtain mixed liquid B, then by mixed liquid B be added decoloration pump in, add mass fraction
For 1% active carbon, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；Destainer is added in concentration pan,
In 65 DEG C of progress concentrations, monosodium glutamate crude product is obtained.
Comparative example 1
A kind of environment-protective process preparing monosodium glutamate comprising following steps:
By Corynebacterium glutamicum seed liquor (1 × 108Cfu/ml) hair equipped with fermentation medium is inoculated according to 8% inoculum concentration
It ferments in fermentation tank, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by certainly
PH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by dynamic Feeding ammonia water；And concentration is added to be the grape of 100g/L by stream
Sugar juice, which controls residual sugar, is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor；Wherein, fermentation medium is
(mass percent): glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, phosphoric acid
Potassium dihydrogen 0.01%, pH6.8；
Glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；Double weight will be added in wet thallus
Dialysis culture base in, 35 DEG C of cultivation temperature, 100rpm stir culture 6h, micro-filtrate membrane filtration collects thallus and filtered solution B；It is described
Dialysis culture base is (mass percent): potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.02%, magnesium sulfate
0.02%, zinc sulfate 0.01%, adjustment pH is 6.8；Above-mentioned microfiltration membranes are inorganic ceramic membrane, molecular cut off 2000Da, micro-filtration
Temperature is 35 DEG C；
Merge filtered solution A and filtered solution B to obtain mixed liquor A, into mixed liquor A, addition accounts for 1/10th parts by weight of mixed liquor A
Sodium carbonate, 100rpm stir 30min, obtain mixed liquid B, then by mixed liquid B be added decoloration pump in, add mass fraction
For 1% active carbon, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；Destainer is added in concentration pan,
In 65 DEG C of progress concentrations, monosodium glutamate crude product is obtained.
Comparative example 2
A kind of environment-protective process preparing monosodium glutamate comprising following steps:
By Corynebacterium glutamicum seed liquor (1 × 108Cfu/ml) hair equipped with fermentation medium is inoculated according to 8% inoculum concentration
It ferments in fermentation tank, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by certainly
PH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by dynamic Feeding ammonia water；And concentration is added to be the grape of 100g/L by stream
Sugar juice, which controls residual sugar, is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor；Wherein, fermentation medium is
(mass percent): glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, phosphoric acid
Potassium dihydrogen 0.01%, pH6.8；
Glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；1wt% hydrogen peroxide is added into wet thallus
And 2wt% tourmaline, 100rpm stir 60min, are then added in the Dialysis culture base of double weight, 35 DEG C of cultivation temperature,
100rpm stir culture 6h, micro-filtrate membrane filtration collect thallus and filtered solution B；The Dialysis culture base is (mass percent): phosphorus
Acid dihydride potassium 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.02%, magnesium sulfate 0.02%, zinc sulfate 0.01%, adjustment pH are
6.8；Above-mentioned microfiltration membranes are inorganic ceramic membrane, and molecular cut off 2000Da, micro-filtration temperature is 35 DEG C；
Merge filtered solution A and filtered solution B to obtain mixed liquor A, into mixed liquor A, addition accounts for 1/10th parts by weight of mixed liquor A
Sodium carbonate, 100rpm stir 30min, obtain mixed liquid B, then by mixed liquid B be added decoloration pump in, add mass fraction
For 1% active carbon, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；Destainer is added in concentration pan,
In 65 DEG C of progress concentrations, monosodium glutamate crude product is obtained.
Comparative example 3
A kind of environment-protective process preparing monosodium glutamate comprising following steps:
By Corynebacterium glutamicum seed liquor (1 × 108Cfu/ml) hair equipped with fermentation medium is inoculated according to 8% inoculum concentration
It ferments in fermentation tank, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by certainly
PH value is maintained 6.8 (0~24 h), 6.5 (24~48 h) by dynamic Feeding ammonia water；And concentration is added to be the grape of 100g/L by stream
Sugar juice, which controls residual sugar, is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor；Wherein, fermentation medium is
(mass percent): glucose 9%, molasses 2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, phosphoric acid
Potassium dihydrogen 0.01%, pH6.8；
Glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；Wet thallus is heated to 55 DEG C, heat preservation
80s, cooled to room temperature are then added in the Dialysis culture base of double weight, and 35 DEG C of cultivation temperature, 100rpm stir culture
6h, micro-filtrate membrane filtration collect thallus and filtered solution B；The Dialysis culture base is (mass percent): potassium dihydrogen phosphate 0.5%, phosphorus
Sour hydrogen dipotassium 0.5%, ferrous sulfate 0.02%, magnesium sulfate 0.02%, zinc sulfate 0.01%, adjustment pH are 6.8；Above-mentioned microfiltration membranes are nothing
Machine ceramic membrane, molecular cut off 2000Da, micro-filtration temperature are 35 DEG C；
Merge filtered solution A and filtered solution B to obtain mixed liquor A, into mixed liquor A, addition accounts for 1/10th parts by weight of mixed liquor A
Sodium carbonate, 100rpm stir 30min, obtain mixed liquid B, then by mixed liquid B be added decoloration pump in, add mass fraction
For 1% active carbon, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；Destainer is added in concentration pan,
In 65 DEG C of progress concentrations, monosodium glutamate crude product is obtained.
Embodiment 3
The production acid amount comparative test of comparative example 1-2 and embodiment 1 has detected glutamic acid so that volume is the mixed liquor A of 100L as an example
Content is specifically shown in Table 1:
Table 1
| Group | Glutamic acid yield g/L |
| Embodiment 1 | 98.6 |
| Comparative example 1 | 85.7 |
| Comparative example 2 | 93.6 |
| Comparative example 3 | 90.7 |
As shown in table 1, compared with comparative example 1-3, the yield of 1 glutamic acid of the embodiment of the present invention be respectively comparative example 1-3 1.15,
1.05 and 1.09 times, prompt processing mode of the present invention that can effectively improve glutamic acid yield.
2, heat treatment temperature produces the influence of acid amount (g/L) to thallus:
It is 40 DEG C, 45 DEG C, 50 DEG C, 55 DEG C and 60 DEG C that temperature, which is set separately, produces glutamic acid to bacterial strain under temperature gradient conditions
It influences, remaining operating procedure is the same as embodiment 1.Concrete outcome is shown in Table 2:
Table 2
| Group | Glutamic acid yield g/L |
| 40℃ | 87.2 |
| 45℃ | 89.7 |
| 50℃ | 97.2 |
| 55℃ | 98.6 |
| 60℃ | 90.6 |
Conclusion: as shown in table 2, as the temperature rises, glutamic acid yield improves, but is increased to after 55 DEG C, and glutamic acid produces
Amount decline, it may be possible to which because the excessively high enzyme activity for causing bacterial strain of temperature declines, and it is larger to cause damage to bacterial strain, it is seen then that
Suitable temperature is more crucial, and temperature is excessively high or too low does not have the effect for improving glutamic acid yield.
Listed above is only best specific embodiment of the invention.It is clear that the invention is not restricted to which above embodiments, can also have
Many deformations.All deformations that those skilled in the art directly can export or associate from present disclosure,
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of environment-protective process for preparing monosodium glutamate comprising following steps: step 1) prepares glutami acid fermentation liquor, step 2 bacterial strain
It recycles, step 3) prepares monosodium glutamate.
2. technique according to claim 1, which is characterized in that the step 1) prepares glutami acid fermentation liquor, including as follows
Step: Corynebacterium glutamicum seed liquor is inoculated in the fermentor equipped with fermentation medium according to 8% inoculum concentration and is sent out
Ferment, 35 DEG C of cultivation temperature, be 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, will by auto-feeding ammonium hydroxide
PH value maintains 6.8 (0~24 h), 6.5 (24~48 h)；And by flowing the glucose solution for adding concentration for 100g/L for residual sugar
Control is being not less than 1.0%, and fermentation to 48h stops, and obtains glutami acid fermentation liquor.
3. technique according to claim 2, which is characterized in that the component of the fermentation medium are as follows: glucose 9%, molasses
2%, corn pulp 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01%, pH6.8, the above are matter
Amount ratio.
4. technique according to claim 2 or 3, which is characterized in that the step 2 bacterial strain recycles, including walks as follows
Rapid: glutami acid fermentation liquor collects wet thallus and filtered solution A through micro-filtrate membrane filtration respectively；1-2wt% dioxygen is added into wet thallus
Water and 2-3wt% tourmaline, 100rpm stir 60min, then stop stirring, are heated to 50-55 DEG C, keep the temperature 60-120s, from
It is so cooled to room temperature, is then added in the Dialysis culture base of double weight, it is 35 DEG C of cultivation temperature, 100rpm stir culture 6h, micro-
Membrane filtration collects thallus and filtered solution B.
5. technique according to claim 4, which is characterized in that the Dialysis culture base are as follows: potassium dihydrogen phosphate 0.5%, phosphoric acid
Hydrogen dipotassium 0.5%, ferrous sulfate 0.02%, magnesium sulfate 0.02%, zinc sulfate 0.01%, adjustment pH are 6.8, and the above are mass ratioes.
6. technique according to claim 4, which is characterized in that the microfiltration membranes are inorganic ceramic membrane, and molecular cut off is
2000Da, micro-filtration temperature are 35 DEG C.
7. technique according to claim 4, which is characterized in that the step 3) prepares monosodium glutamate, includes the following steps: to filter
It crosses liquid A and filtered solution B merges to obtain mixed liquor A, into mixed liquor A, addition accounts for the sodium carbonate of 1/10th parts by weight of mixed liquor A,
100rpm stirs 30min, obtains mixed liquid B, and then mixed liquid B is added in decoloration pump, adds the work that mass fraction is 1%
Property charcoal, decolourize 30min；10min is centrifuged with 3000rpm again, collects destainer；By destainer be added concentration pan in, in 65 DEG C into
Row concentration obtains monosodium glutamate crude product.
8. being allowed to the msg product of technique preparation according to claim 1-7.
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Application publication date: 20190524 |