CN109793236B - Preparation method of noni extract - Google Patents
Preparation method of noni extract Download PDFInfo
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- CN109793236B CN109793236B CN201910265190.2A CN201910265190A CN109793236B CN 109793236 B CN109793236 B CN 109793236B CN 201910265190 A CN201910265190 A CN 201910265190A CN 109793236 B CN109793236 B CN 109793236B
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Abstract
The invention provides a preparation method of a noni extract, which comprises the following steps of S1, taking fresh noni, pre-freezing for 4-6 h at-10 to-5 ℃; then placing the mixture into a mixed solution of sodium chloride and acetic acid, soaking for 20-40min at 50-70 ℃, and pulping to obtain noni pulp; s2, placing noni pulp in 2-4 times volume of ethanol solution, performing strong electric field extraction, wherein the electric field intensity is 2.0-2.3 kV, the pulse frequency is 2000-2200 Hz, the electrode spacing is 2-4 mm, and extracting for 40-50 min to obtain an extract liquid; s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract. The noni fruit extract prepared by the invention has high noni polyphenol and noni total flavone content, and has DPPH free radical removal rate and ABTS+The free radical removal rate is high, the antioxidant capacity is high, and the method can be applied to preparation of antioxidant products.
Description
Technical Field
The invention relates to the technical field of noni fruits, and particularly relates to a preparation method of a noni fruit extract.
Background
Noni, belonging to the genus morinda of the family rubiaceae. At present, China is mainly distributed in heat zone areas such as Hainan area, Xisha islands and the like, and large-scale planting is carried out. The noni fruit has rich nutrient substances and high medical value, and can be used as a food-grade health product. However, the noni fruit has a special taste and is loved by people, extraction of active ingredients in the noni fruit is an important way for effectively exerting the efficacy of the noni fruit, noni polyphenol and flavone are important active ingredients of the noni fruit, the extraction method mainly comprises an extraction method, supercritical fluid extraction, an enzymolysis method, an ultrasonic method and a microwave method at present, but the noni fruit extract prepared by the existing method has low content of the active ingredients, so that the application effect is not strong, and improvement of the preparation method of the extract is urgently needed.
Disclosure of Invention
Therefore, the invention provides a preparation method of the noni extract, which solves the technical problems.
The technical scheme of the invention is realized as follows:
a preparation method of noni fruit extract comprises the following steps:
s1, pre-freezing fresh noni fruits at-10 to-5 ℃ for 4 to 6 hours; then placing the mixture into a mixed solution of sodium chloride and acetic acid, soaking for 20-40min at 50-70 ℃, and pulping to obtain noni pulp;
s2, placing noni pulp in 2-4 times volume of ethanol solution, performing strong electric field extraction, wherein the electric field intensity is 2.0-2.3 kV, the pulse frequency is 2000-2200 Hz, the electrode spacing is 2-4 mm, and extracting for 40-50 min to obtain an extract liquid;
s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract.
Further, in the step S1, the mixed solution of sodium chloride and acetic acid contains 5-10 w/w% of sodium chloride and 1-3 w/w% of acetic acid.
Further, in the step S1, the concrete operation of beating: beating at 300-500rpm for 1-2 min, and then beating at 1000-2000 rpm for 3-5 min.
Further, in the step S2, the volume concentration of the ethanol solution is 55-65%.
Further, in the step S1, the mixed solution of sodium chloride and acetic acid contains 8 w/w% of sodium chloride and 2 w/w% of acetic acid.
Further, in the step S2, the ethanol solution has a volume concentration of 60%.
Further, in step S2, the conditions of the strong electric field extraction are as follows: the electric field intensity is 2.2kV, the pulse frequency is 2100Hz, the electrode spacing is 3mm, and the extraction is carried out for 45 min.
Compared with the prior art, the invention has the beneficial effects that: the invention combines pre-freezing, strong electric field extraction and drying, scientifically sets process parameters, and the prepared noni fruit extract has high noni polyphenol and noni total flavone content, high DPPH free radical removal rate and ABTS+The free radical removal rate is high, the antioxidant capacity is high, and the method can be applied to preparation of antioxidant products. Wherein the invention uses prefreezingThe method combines high-temperature salt vinegar soaking to fully break tissue cells, so that active ingredients are fully dissolved in a noni pulp system, the strong electric field extraction efficiency is improved, and then the purity of the active ingredients in the extract is fully improved by combining a strong electric field extraction process. In addition, the low-high speed pulping method provided by the invention further improves active ingredients in a noni pulp system and improves the extraction efficiency of a strong electric field; the volume concentration of the ethanol solution provided by the invention further improves the extraction efficiency of the strong electric field and improves the content of active ingredients of the noni extract.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A preparation method of noni fruit extract comprises the following steps:
s1, pre-freezing fresh noni fruits at-10 ℃ for 4 hours; placing in mixed solution of sodium chloride and acetic acid, wherein the mixed solution of sodium chloride and acetic acid contains 5 w/w% of sodium chloride and 1 w/w% of acetic acid, soaking at 50 deg.C for 40min, pulping at 300rpm for 2min, and pulping at 1000rpm for 5min to obtain noni pulp;
s2, placing noni pulp in a 65 v/v% ethanol solution with the volume 2 times that of the noni pulp, extracting for 50min by using a strong electric field with the electric field intensity of 2.0kV, the pulse frequency of 2000Hz and the electrode spacing of 2mm to obtain an extraction liquid;
s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract.
Example 2
A preparation method of noni fruit extract comprises the following steps:
s1, pre-freezing fresh noni fruits at-5 ℃ for 6 hours; placing in a mixed solution of sodium chloride and acetic acid, wherein the mixed solution of sodium chloride and acetic acid contains 10 w/w% of sodium chloride and 3 w/w% of acetic acid, soaking at 70 deg.C for 20min, pulping at 500rpm for 1min, and pulping at 2000rpm for 3min to obtain noni pulp;
s2, placing noni pulp in 55 v/v% ethanol solution with the volume 4 times that of the noni pulp, extracting for 40min in a strong electric field with the electric field intensity of 2.3kV, the pulse frequency of 2200Hz and the electrode spacing of 4mm to obtain extract liquor;
s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract.
Example 3
A preparation method of noni fruit extract comprises the following steps:
s1, pre-freezing fresh noni fruits at-8 ℃ for 5 hours; placing in mixed solution of sodium chloride and acetic acid, wherein the mixed solution of sodium chloride and acetic acid contains 8 w/w% of sodium chloride and 2 w/w% of acetic acid, soaking at 60 deg.C for 30min, pulping at 400rpm for 1min, and pulping at 1500rpm for 4min to obtain noni pulp;
s2, placing noni pulp in a 60 v/v% ethanol solution with the volume 3 times that of the noni pulp, extracting for 45min by using a strong electric field, wherein the electric field intensity is 2.2kV, the pulse frequency is 2100Hz, and the electrode spacing is 3mm to obtain an extract liquid;
s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract.
Example 4
This example is different from example 3 in that, in the step S1, the mixed solution of sodium chloride and acetic acid contains 20 w/w% of sodium chloride and 5 w/w% of acetic acid.
Example 5
This example is different from example 3 in that in the step of S1, the beating is carried out directly at 2000rpm for 5 min.
Example 6
This example is different from example 3 in that, in the step of S2, the volume concentration of the ethanol solution is 40%.
Comparative example 1
This comparative example is different from example 3 in that the slurry is directly soaked and beaten without prefreezing in the step S1.
Comparative example 2
This comparative example is different from example 3 in that the mixed solution of sodium chloride and acetic acid was replaced with a 8 w/w% sodium chloride solution without acetic acid in the step of S1.
Comparative example 3
This comparative example is different from example 3 in that the soaking temperature is 80 c in the S1 step.
Comparative example 4
This comparative example differs from example 3 in that in the step of S2, the strong electric field extraction is replaced with microwave extraction.
Comparative example 5
This comparative example is different from example 3 in that in the step S2, the electric field strength was 2.4kV, the pulse frequency was 2300Hz, and the electrode spacing was 3 mm.
Firstly, the noni polyphenol and noni total flavone contents in the noni fruit extracts prepared in the embodiments and the comparative proportion are detected and compared with the noni fruit extract prepared by the traditional method, and the results are as follows:
| noni polyphenol (%) | Noni total flavone (%) | |
| Example 1 | 3.65 | 4.29 |
| Example 2 | 3.49 | 4.31 |
| Example 3 | 3.84 | 4.73 |
| Example 4 | 3.78 | 4.62 |
| Example 5 | 3.69 | 4.58 |
| Example 6 | 3.75 | 4.64 |
| Comparative example 1 | 2.64 | 3.09 |
| Comparative example 2 | 2.77 | 3.12 |
| Comparative example 3 | 2.82 | 3.34 |
| Comparative example 4 | 2.78 | 3.29 |
| Comparative example 5 | 2.92 | 3.73 |
| Conventional methods | ＜2.50 | ＜2.70 |
The results show that the noni fruit extract prepared by the invention has high noni polyphenol and noni total flavone content.
Secondly, the noni fruit extracts prepared in the examples have DPPH free radical removal rate and ABTS+And (3) detecting the removal rate of free radicals, and comparing the free radicals with the noni extract prepared by the traditional method:
(1) determination of DPPH radical scavenging Rate
Preparing a DPPH solution with the mass concentration of 0.20mg/mL, and storing the DPPH solution in a refrigerator in a dark place for later use. Taking 2.00mL of samples with different mass concentrations, adding the prepared DPPH solution, shaking up, and reacting for 30min in a dark place. The absorbance value was measured at a wavelength of 517nm with 100% volume fraction of ethanol as a reference. The DPPH radical clearance calculation formula is as follows:
DPPH radical scavenging ratio/% - [ A ]i-(Ax-Ae)]/Ai×100％
In the formula: a. theiIs the absorbance value of DPPH solution; a. thexThe absorbance values of the sample solution and the DPPH solution are obtained; a. theeThe absorbance of the sample solution was measured in the absence of DPPH.
(2)ABTS+Determination of free radicals
Determination of ABTS by spectrophotometry+Radical removal rate. Mixing 2.00mL of sample solution with different concentrations and 8.00mL of Using solution of LaBTS, mixing, placing in dark for 30min, and measuring absorbance at wavelength of 517nm with distilled water as reference. ABTS+The formula for radical clearance is:
ABTS+radical scavenging rate/% - [ A ]1-(A0-A2)]/A1×100
In the formula: a. the1Is ABTS+The absorbance value of the free radical solution; a. the0For the sample liquid and ABTS+The absorbance value of the free radical solution; a. the2Is free of ABTS+Absorbance value of sample liquid in free radical solution.
Removal rate of DPPH free radical, ABTS+The radical removal results were as follows:
| DPPH radical removal Rate (%) | ABTS+Radical removal Rate (%) | |
| Example 1 | 94.88 | 91.84 |
| Example 2 | 95.96 | 92.16 |
| Example 3 | 99.18 | 94.87 |
| Example 4 | 97.47 | 92.65 |
| Example 5 | 98.38 | 93.14 |
| Example 6 | 97.52 | 92.28 |
| Conventional methods | ＜90 | ＜80 |
The results show that the noni fruit extract prepared by the invention has DPPH free radical removal rate and ABTS+The removal rate of free radicals is high, and the antioxidant capacity is strong.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. A preparation method of noni fruit extract is characterized by comprising the following steps: the method comprises the following steps:
s1, pre-freezing fresh noni fruits at-10 to-5 ℃ for 4 to 6 hours; then placing the mixture into a mixed solution of sodium chloride and acetic acid, wherein the mixed solution of sodium chloride and acetic acid contains 5-10 w/w% of sodium chloride and 1-3 w/w% of acetic acid, soaking for 20-40min at 50-70 ℃, and then pulping, wherein the specific operation of pulping is as follows: pulping at 500rpm for 1-2 min and then at 1000-2000 rpm for 3-5 min to obtain noni pulp;
s2, placing noni pulp in 2-4 times volume of ethanol solution, performing strong electric field extraction, wherein the electric field intensity is 2.0-2.3 kV, the pulse frequency is 2000-2200 Hz, the electrode spacing is 2-4 mm, and extracting for 40-50 min to obtain an extract liquid;
s3, centrifuging the extractive solution, and freeze drying to obtain Morinda citrifolia extract.
2. The method for preparing noni fruit extract according to claim 1, wherein: in the step S2, the volume concentration of the ethanol solution is 55-65%.
3. The method for preparing noni fruit extract according to claim 1, wherein: in the step S1, the mixed solution of sodium chloride and acetic acid contains 8 w/w% of sodium chloride and 2 w/w% of acetic acid.
4. The method for preparing noni fruit extract according to claim 2, wherein: in the step S2, the ethanol solution has a volume concentration of 60%.
5. The method for producing a noni fruit extract according to any one of claims 1 to 4, wherein: in the step S2, the conditions of the strong electric field extraction are as follows: the electric field intensity is 2.2kV, the pulse frequency is 2100Hz, the electrode spacing is 3mm, and the extraction is carried out for 45 min.
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| CN106749746A (en) * | 2017-02-27 | 2017-05-31 | 云南省热带作物科学研究所 | It is a kind of to improve the method and extract obtained and application for obtaining the beautiful polysaccharide of Noni fruit promise |
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| CN101692906A (en) * | 2009-10-16 | 2010-04-14 | 国占武 | Apple polyphenol extracting method |
| CN106083796A (en) * | 2016-06-20 | 2016-11-09 | 仇颖莹 | A kind of extracting method of vaccinium oxycoccus pigment |
| CN106692389A (en) * | 2017-02-27 | 2017-05-24 | 云南省热带作物科学研究所 | Method for acquiring functional components in morinda citrifolia as well as extract and application |
| CN106749746A (en) * | 2017-02-27 | 2017-05-31 | 云南省热带作物科学研究所 | It is a kind of to improve the method and extract obtained and application for obtaining the beautiful polysaccharide of Noni fruit promise |
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