CN109777148A - A kind of pumpkin marennin and its extracting method - Google Patents
A kind of pumpkin marennin and its extracting method Download PDFInfo
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- CN109777148A CN109777148A CN201910088654.7A CN201910088654A CN109777148A CN 109777148 A CN109777148 A CN 109777148A CN 201910088654 A CN201910088654 A CN 201910088654A CN 109777148 A CN109777148 A CN 109777148A
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Abstract
The present invention provides a kind of pumpkin marennin and its extracting method, specific extracting method includes: that the pumpkin after decladding, selection by winnowing, machine choosing is carried out microwave drying again.Then low-toxic solvent is added to be extracted by impulse electric field processing unit simultaneously, a leaching liquor is obtained by filtration in feed liquid；Low-toxic organic solvent is added in filter residue again, repeats to extract, secondary leaching liquor is obtained after filtering；Merge twice leaching liquor to get arrive pumpkin marennin leaching liquor.Powdered pumpkin marennin will be obtained after the leaching liquor being collected into centrifugation, filtering, concentration, drying.The present invention uses low-toxic solvent as Extraction solvent, green, environmental protection, recovery rate height；The resulting pumpkin marennin pure color of the present invention, color value are high, highly-safe, can be used as natural pigment for food service industry.Pumpkin added value of product can be improved in the present invention, while retaining the original natural form of pumpkin, is convenient for next link processing technology, the value with large-scale promotion and application.
Description
Technical field
The invention belongs to nature strength field, in particular to a kind of pumpkin marennin and its extracting method.
Background technique
Edible pigment can be divided into natural and artificial coloring's two major classes, and natural pigment is highly-safe, and tone is soft, and some
With certain physiological activity, belong to functional natural colorants.Pigment kind Green element is less at present, but market demand phase
To larger.Pumpkin is the mature seed of cucurbitaceous plant pumpkin.Pumpkin essential fatty acid rich in, amino
The bioactive substances such as acid, phytosterol, polyphenol, vitamin and minerals, have prevention prostatic disorders, adjust blood glucose,
The effect of adjusting cholesterol.In recent years, not with the rise of domestic and international pumpkin process deeply industry and pumpkin export volume
Disconnected to increase, the cultivated area of China's seed-use pumpkin expands year by year, and production and processing at present is concentrated mainly on Heilongjiang Province, the Inner Mongol certainly
Area and Gansu Province are controlled, the green matter of discovery pumpkin seed surface can be attached to work people in election process in pumpkin classification
On the gloves of member, and green color is just, and colorability is strong, thus the one kind of pumpkin marennin as natural pigment, has huge
Big exploitation potential and value.
Traditional nature strength technology has the characteristics that energy consumption is high, largely uses petro chemical solvent and pollution environment.With
China is continuously increased the attention of environmental problem and people to " green " product demand, and natural pigment green extraction is more next
More it is valued by people.
Impulse electric field (Pulsed Electric Field, PEF) is a kind of non-heat treated technology, it is in two electrodes
Between place the repetitive short pulses high-voltage pulse of biomaterial (plant, animal or microbial cell) a kind of, this process can
Make the pigment for being firmly attached to pumpkin seed seed coat surface completely fall off, at the same retain pumpkin integrality and with tradition
Solvent extraction method compare, extraction time can be shortened, improve recovery rate, impulse electric field is in pumpkin marennin extraction side at present
The application in face has no that related patents are reported.
Summary of the invention
In view of the above problems, the object of the present invention is to provide a kind of pumpkin marennin and its extracting methods.It is logical
It crosses green extraction to be extracted, new marennin source is provided for the mankind, while retaining the original natural shape of pumpkin
State is convenient for next link processing technology.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of extracting method of pumpkin marennin, comprising the following steps:
(1) after cleaning up the pumpkin after decladding, selection by winnowing, machine choosing, microwave drying, the pumpkin after being dried are carried out；
(2) using low-toxic organic solvent as extractant, the pumpkin after drying obtained in step (1) is added is mixed, by
Impulse electric field carries out assisted extraction, and a leaching liquor is obtained by filtration in feed liquid；Filter residue is uniformly mixed with low-toxic organic solvent,
Impulse electric field assisted extraction is used again, and feed liquid is using obtaining secondary leaching liquor after filter；It is southern to get arriving to merge leaching liquor twice
Melon seeds marennin leaching liquor.
Further, the method also includes following steps:
(3) pumpkin marennin leaching liquor will be obtained, successively by centrifugation, filtering, concentration, drying and processing, will be obtained powdered
Pumpkin marennin.
Further, the condition of microwave drying described in step (1) are as follows: power be 420 ~ 450W, the time be 5 ~
10min。
Further, low-toxic organic solvent described in step (2) is one in dehydrated alcohol, ethyl acetate and petroleum ether
Kind or several mixtures.
Further, the low-toxic organic solvent is the mixture of dehydrated alcohol, ethyl acetate and petroleum ether, and anhydrous
The volume ratio of ethyl alcohol, ethyl acetate and petroleum ether is 4 ~ 6:3:1 ~ 3.
Further, the solid-liquid ratio of pumpkin and low-toxic organic solvent is 1g:1 ~ 5ml in the step (2).
Further, impulse electric field strength is 60kv/cm, 30 μ s of pulse width in the step (2), and pulse frequency is
1000Hz, operating temperature are room temperature, working time 2h.
A kind of pumpkin marennin, the pumpkin marennin are that above-mentioned extracting method is extracted.
The present invention having the beneficial effect that compared with prior art
1, the extracting method of pumpkin marennin of the present invention, belongs to green extraction, extends the comprehensive benefit of pumpkin
With range, the bore of pumpkin secondary industry has been widened, has retained the original natural form of pumpkin, has processed skill convenient for next link
Art；
2, the extracting method of pumpkin marennin of the present invention, agents useful for same is that the green of hypotoxicity has in leaching process
Solvent, and can recycle, the safety of natural pigment is improved, environmental pollution is reduced；In addition, common solvent extraction mentions
Pigment is taken to cook extractant with ethyl alcohol, and less toxic organic solvent --- the dehydrated alcohol, acetic acid that the present invention selects three kinds of polarity different
Ethyl ester and petroleum ether, it is therefore an objective to can sufficiently extract pumpkin marennin while safety low-poison；
3, pulse electric field technology is introduced the extraction of pumpkin marennin by the extracting method of pumpkin marennin of the present invention
In technique, compared with traditional solvent extraction method, there is short processing time, recovery rate height, low power consumption and other advantages, be conducive to pumpkin
The large-scale promotion and application of the green extraction of seed marennin；
4, the resulting pumpkin marennin pure color of the present invention, color value are high, highly-safe；And raw material used in the present invention ---
Pumpkin has better nutritivity edible value, has no toxic side effect, can be used as a kind of extraction raw material of ideal marennin.
Detailed description of the invention
Fig. 1 is the process flow chart of the extracting method of pumpkin marennin of the present invention；
Fig. 2 is different extractants to the pumpkin marennin extraction effect comparison diagram.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but the invention is not limited to these embodiments.
Embodiment 1
As shown in Figure 1, present embodiments providing a kind of pumpkin marennin, and extracted by green extraction method,
The specific method is as follows:
(1) pumpkin after decladding, selection by winnowing, machine choosing is cleaned up into carry out microwave drying, microwave power 450W, the time is
10min；
(2) by food-grade anhydrous ethyl alcohol, ethyl acetate and petroleum ether, 4:3:3 is uniformly mixed that form hypotoxicity organic molten by volume
Agent, as extractant, by after drying pumpkin seed and extractant be uniformly mixed in the ratio of 1g:1m L, using impulse electric field
A leaching liquor is obtained by filtration in assisted extraction, feed liquid；Filter residue is uniformly mixed with above-mentioned low-toxic organic solvent, is used again
Impulse electric field assisted extraction, feed liquid is using obtaining secondary leaching liquor after filter；Merge twice leaching liquor to get pumpkin marennin
Leaching liquor；
Wherein, impulse electric field strength is 60kv/cm, pulse width 30 μ s, pulse frequency 1000Hz, work in leaching process twice
Making temperature is room temperature, working time 2h；
(3) obtained pumpkin marennin leaching liquor is centrifuged 10min under conditions of revolving speed is 5000r/min, takes supernatant
It carries out being concentrated under reduced pressure into extracting solution density being 1.2g/cm3To get pumpkin marennin concentrate is arrived, in concentration process simultaneously
Recycle organic solvent；
(4) obtained pumpkin marennin concentrate will be collected and carries out drying and processing, obtain powdered pumpkin marennin.
Through detecting, the pumpkin marennin recovery rate that above-described embodiment obtains is 0.84%, measures green color index through color difference meter
It is 6.78.Pumpkin marennin quality/pumpkin quality * 100% that the recovery rate=extraction obtains.
Embodiment 2
As shown in Figure 1, present embodiments providing a kind of pumpkin marennin, and extracted by green extraction,
The specific method is as follows:
(1) pumpkin after decladding, selection by winnowing, machine choosing is cleaned up into carry out microwave drying, microwave power 450W, the time is
10min；
(2) by food-grade anhydrous ethyl alcohol, ethyl acetate and petroleum ether, 6:3:1 is uniformly mixed that form hypotoxicity organic molten by volume
Agent, as extractant, by after drying pumpkin seed and extractant be uniformly mixed in the ratio of 1g:1m L, using impulse electric field
A leaching liquor is obtained by filtration in assisted extraction, feed liquid；Filter residue is uniformly mixed with above-mentioned low-toxic organic solvent, is used again
Impulse electric field assisted extraction, feed liquid is using obtaining secondary leaching liquor after filter；Merge twice leaching liquor to get pumpkin marennin
Leaching liquor, wherein twice in leaching process impulse electric field strength be 60kv/cm, pulse width 30 μ s, pulse frequency 1000Hz,
Operating temperature is room temperature, working time 2h；
(3) obtained pumpkin marennin leaching liquor is centrifuged 10min under conditions of revolving speed is 5000r/min, takes supernatant
It carries out being concentrated under reduced pressure into extracting solution density being 1.15g/cm3To get pumpkin marennin concentrate is arrived, in concentration process simultaneously
Recycle organic solvent；
(4) obtained pumpkin marennin concentrate will be collected and carries out drying and processing, obtain powdered pumpkin marennin.
Through detecting, the pumpkin marennin recovery rate that examples detailed above obtains is 0.82%, is through color difference meter measurement green color index
6.92。
Embodiment 3
As shown in Figure 1, present embodiments providing a kind of pumpkin marennin, and extracted by green extraction,
The specific method is as follows:
(1) pumpkin after decladding, selection by winnowing, machine choosing is cleaned up into carry out microwave drying, microwave power 430W, the time is
10min；
(2) by food-grade anhydrous ethyl alcohol, ethyl acetate and petroleum ether, 5:3:2 is uniformly mixed by volume, as extractant, will be done
Pumpkin seed and extractant after dry are uniformly mixed in the ratio of 1g:1mL, and using impulse electric field assisted extraction, feed liquid is through filtering
Obtain a leaching liquor；Filter residue is uniformly mixed with low-toxic organic solvent, uses impulse electric field assisted extraction again, feed liquid is again
Secondary leaching liquor is obtained after filtering；Leaching liquor is twice to get pumpkin marennin leaching liquor for merging, wherein leaching process twice
Middle impulse electric field strength is 60kv/cm, and 30 μ s of pulse width, pulse frequency 1000Hz, operating temperature is room temperature, working time
For 2h；
(3) obtained pumpkin marennin leaching liquor is centrifuged 10min under conditions of revolving speed is 5000r/min, takes supernatant
It carries out being concentrated under reduced pressure into extracting solution density being 1.18g/cm3To get pumpkin marennin concentrate is arrived, in concentration process simultaneously
Recycle organic solvent；
(4) obtained pumpkin marennin concentrate will be collected and carries out drying and processing, obtain powdered pumpkin marennin.
Through detecting, the pumpkin marennin recovery rate that examples detailed above obtains is 0.82%, is through color difference meter measurement green color index
6.45。
Comparative example 1
To investigate influence of the extractant to pumpkin marennin recovery rate and green color index, this comparative example is basic according to embodiment 1
Identical method extracts pumpkin marennin, and difference is: extractant selects dehydrated alcohol, ethyl acetate and petroleum ether respectively,
It extracts and obtains the pumpkin marennin, and compared together with the pumpkin marennin that embodiment 1-3 method obtains, 6
Kind extractant carries out full wavelength scanner result respectively as shown in Fig. 2, as shown in Figure 2, the pigment is within the scope of wavelength 420-425nm
Absorbance highest, and the mixing that dehydrated alcohol used in embodiment 1-3, ethyl acetate and petroleum ether are mixed in a certain ratio mentions
Take extraction effect close, and better than single solvent extraction effect.
Comparative example 2
Ultrasonic wave added solvent extraction pumpkin marennin is taken in this comparative example, specific method the following steps are included:
(1) pumpkin after decladding, selection by winnowing, machine choosing is cleaned up into carry out microwave drying, microwave power 430W, the time is
10min；
(2) by food-grade anhydrous ethyl alcohol, ethyl acetate and petroleum ether, 5:3:2 is uniformly mixed by volume, as extractant, will be done
Pumpkin seed and extractant after dry are uniformly mixed in the ratio of 1g:1mL, and using ultrasonic extraction, feed liquid is obtained by filtration once
Leaching liquor；Filter residue is uniformly mixed with low-toxic organic solvent, uses ultrasonic extraction again, feed liquid is secondary using obtaining after filter
Leaching liquor；Merge twice leaching liquor to get pumpkin marennin leaching liquor, wherein twice in leaching process ultrasonic extraction time
For 20min, ultrasonic power: 600w；
(3) obtained pumpkin marennin leaching liquor is centrifuged 10min under conditions of revolving speed is 5000r/min, takes supernatant
It carries out being concentrated under reduced pressure into extracting solution density being 1.18g/cm3To get pumpkin marennin concentrate is arrived, in concentration process simultaneously
Recycle organic solvent；
(4) obtained pumpkin marennin concentrate will be collected and carries out drying and processing, obtain powdered pumpkin marennin.
Through detecting, pumpkin marennin recovery rate made from above-mentioned comparative example is 0.45%, measures green color index through color difference meter
It is 5.92.By comparison it is found that impulse electric field assisted extraction of the present invention, is greatly improved the recovery rate of pumpkin marennin, and
The green color index of obtained marennin is also more excellent.
Although the present invention has been disclosed in the preferred embodiment as above, it is not intended to limit the invention, any to be familiar with this
The people of technology can do various changes and modification, therefore protection of the invention without departing from the spirit and scope of the present invention
Range should subject to the definition of the claims.
Claims (9)
1. a kind of extracting method of pumpkin marennin, which is characterized in that the extracting method the following steps are included:
(1) pumpkin after decladding, selection by winnowing, machine choosing is subjected to microwave drying, the pumpkin after being dried；
(2) using low-toxic organic solvent as extractant, the pumpkin after drying obtained in step (1) is added is mixed, by
Impulse electric field carries out assisted extraction, and a leaching liquor is obtained by filtration in feed liquid；Filter residue is uniformly mixed with low-toxic organic solvent,
Impulse electric field assisted extraction is used again, and feed liquid is using obtaining secondary leaching liquor after filter；It is southern to get arriving to merge leaching liquor twice
Melon seeds marennin leaching liquor.
2. the extracting method of pumpkin marennin according to claim 1, which is characterized in that the method also includes following
Step:
(3) pumpkin marennin leaching liquor will be obtained, successively by centrifugation, filtering, concentration, drying and processing, will be obtained powdered
Pumpkin marennin.
3. the extracting method of pumpkin marennin according to claim 1, which is characterized in that microwave described in step (1)
Dry condition are as follows: power is 420 ~ 450W, and the time is 5 ~ 10min.
4. the extracting method of pumpkin marennin according to claim 1, which is characterized in that low toxicity described in step (2)
Property organic solvent be one or more of dehydrated alcohol, ethyl acetate and petroleum ether mixture.
5. the extracting method of pumpkin marennin according to claim 4, which is characterized in that the low-toxic organic solvent
For the mixture of dehydrated alcohol, ethyl acetate and petroleum ether, and the volume ratio of dehydrated alcohol, ethyl acetate and petroleum ether be 4 ~
6:3:1 ~ 3。
6. the extracting method of pumpkin marennin according to claim 5, which is characterized in that the dehydrated alcohol, acetic acid
The volume ratio of ethyl ester and petroleum ether is 3:2:5.
7. the extracting method of pumpkin marennin according to claim 1, which is characterized in that pumpkin in the step (2)
The solid-liquid ratio of seed and low-toxic organic solvent is 1g:1 ~ 5ml.
8. the extracting method of pumpkin marennin according to claim 1, which is characterized in that pulse in the step (2)
Electric field strength is 60kv/cm, and 30 μ s of pulse width, pulse frequency 1000Hz, operating temperature is room temperature, working time 2h.
9. a kind of pumpkin marennin, which is characterized in that the pumpkin marennin is described in any item by claim 1-8
What extracting method was extracted.
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| CN201910088654.7A CN109777148A (en) | 2019-01-30 | 2019-01-30 | A kind of pumpkin marennin and its extracting method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297469A (en) * | 1998-05-11 | 2001-05-30 | 西格弗里德·彼得 | Process for extracting carotenes from carotene-contg. materials |
| CN105348850A (en) * | 2015-11-06 | 2016-02-24 | 南宁邃丛赋语科技开发有限责任公司 | Extraction method of peanut red skin pigment |
| CN106350199A (en) * | 2016-08-29 | 2017-01-25 | 界首市宏源家庭农场 | Pumpkin seed oil extraction method |
-
2019
- 2019-01-30 CN CN201910088654.7A patent/CN109777148A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297469A (en) * | 1998-05-11 | 2001-05-30 | 西格弗里德·彼得 | Process for extracting carotenes from carotene-contg. materials |
| CN105348850A (en) * | 2015-11-06 | 2016-02-24 | 南宁邃丛赋语科技开发有限责任公司 | Extraction method of peanut red skin pigment |
| CN106350199A (en) * | 2016-08-29 | 2017-01-25 | 界首市宏源家庭农场 | Pumpkin seed oil extraction method |
Non-Patent Citations (5)
| Title |
|---|
| 刘曦然 等: "高压脉冲电场在提取天然产物中的应用", 《食品工业》 * |
| 吴晓磊 等: "南瓜籽油的化学成分与药理作用", 《现代药物与临床》 * |
| 孙欣 等: "超声波法提取南瓜籽油的研究", 《中国粮油学报》 * |
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