CN109771455A - Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease - Google Patents
Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease Download PDFInfo
- Publication number
- CN109771455A CN109771455A CN201910233587.3A CN201910233587A CN109771455A CN 109771455 A CN109771455 A CN 109771455A CN 201910233587 A CN201910233587 A CN 201910233587A CN 109771455 A CN109771455 A CN 109771455A
- Authority
- CN
- China
- Prior art keywords
- extract
- preparation
- trametes robinioplila
- trametes
- robinioplila
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of fungi extracts in the purposes of purposes more particularly to trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease.Of the invention preparation method is simple, operating cost is low, Extraction solvent in preparation method of the invention can remain risk with recycling and reusing, no organic reagent, it is while ensure that the natural quality of trametes robinioplila extract and environmentally friendly, belong to green manufacture technology.Raw material extraction is more complete, takes full advantage of raw material, raw material availability is also very high on the basis of guaranteeing extract effect.Trametes robinioplila extract of the invention can not only be clearly helpful for the reparation that high fat diet causes hepatic injury, but also significantly treatment and/or preventive effect are shown to disease relevant to hepatic injury, it can be used in the various fields such as drug, health care product, there is great economy and society value.
Description
Technical field
The present invention relates to a kind of fungi extracts to cause hepatic injury related disease in preparation treatment and/or prevention high fat diet
Preparation in purposes more particularly to trametes robinioplila extract preparation treatment and/or prevention high fat diet cause hepatic injury related disease
Preparation in purposes.
Background technique
Enteric microorganism group is known as " second set of genome of human body ", closely bound up with human health.The micro- life of human intestine
Up to 1000 kinds or more of species, wherein Firmicutes and Bacteroidetes are dominant microflora, the gene dosage in enteron aisle genome
It is more than 100 times of mankind's autogene group.There is mutualism relationships by intestinal flora and host, and dynamic is remain in enteron aisle
Balance, when balance is broken, people is possible to suffer from various diseases.Existing research confirms: (1) intestinal flora and fat phase
It closes, there are intestinal microflora imbalance, Phylogenetic diversity of bacteria declines for obese people；The Bacteroidetes of obesity mice is few, Firmicutes
It is more；The enteric microorganism of obesity mice is transplanted on germfree mouse, will lead to the increase of the latter's fat mass.Fat people is come
It says, takes in high fat diet for a long time, the change of intestinal microflora can be caused, unbalance intestinal flora can generate endotoxin and enter people
Body generates multiple inflammatory factors after being identified by immunocyte, and body is caused to be in low grade inflammation state, generates metabolic disorder, makes
It obtains the energy absorbed from food and is easily converted to fat, cause obesity；(2) liver and enteron aisle are close on biological function
It is related, interactional.Liver and intestinal flora are interacted by intestines-liver axis, and intestinal flora is close with liver diseases, liver
When illness, the structure and function of intestinal flora will affect, and can influence liver function when intestinal bacilli illness in turn, liver is hard
Changing the beneficial bacteriums quantity such as patient Bifidobacterium reduces, and the harmful bacterias quantity such as enterobacteria, enterococcus increases.
High fat diet can change intestinal microflora, reduce beneficial bacterium, and harmful bacteria increases, and destroy the flat of intestinal flora
Weighing apparatus, and then liver function is influenced, hepatic injury is caused, and increasing the problems such as with the development and dietary structure of China's living standard
Add, the disease incidence of the hepatic injury as caused by high fat diet obviously increases, and there is an urgent need to develop treat and/or prevent high in fat in preparation
Diet causes the preparation in terms of hepatic injury related disease.
Summary of the invention
Against the above deficiency, the present invention provides a kind of trametes robinioplila extract and causes liver in preparation treatment and/or prevention high fat diet
Damage related disease preparation in purposes, function is significant clear, can medicinalization, have practicability.
The present invention reaches above-mentioned purpose by following scheme:
In the first aspect of the present invention, a kind of trametes robinioplila extract is provided treating and/or prevent high fat diet and causes hepatic injury
Purposes in related disease.
In the second aspect of the present invention, a kind of trametes robinioplila extract is provided in preparation treatment and/or prevention high fat diet and causes liver
Damage the purposes in the preparation of related disease.
In the third aspect of the present invention, a kind of system treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Agent, the preparation include trametes robinioplila extract.
In the fourth aspect of the present invention, a kind of medicine treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Object includes trametes robinioplila extract and pharmaceutically acceptable carrier.
In the fifth aspect of the invention, a kind of preparation method of trametes robinioplila extract is provided, comprising: crush trametes robinioplila fructification
Afterwards, it is successively mentioned with alcohol extracting, water, the extraction of NaOH buck, respectively obtains extraction solution, then concentrated, freeze-drying respectively, obtain Chinese scholartree
Ear alcohol extracting thing, trametes robinioplila water extract, trametes robinioplila alkali extract.
Compared with prior art, the invention has the following beneficial effects:
(1) of the invention preparation method is simple, operating cost is low, and the Extraction solvent in preparation method of the invention is only
Ethyl alcohol, water, NaOH aqueous solution have been used, risk can have been remained with recycling and reusing, no organic reagent, ensure that trametes robinioplila extract
It is while natural quality and environmentally friendly, belong to green manufacture technology.
(2) present invention successively uses ethyl alcohol, water and NaOH extraction with aqueous solution trametes robinioplila fructification, and raw material extraction is more complete, fills
Divide and raw material is utilized, raw material availability is also very high on the basis of guaranteeing extract effect.
(3) trametes robinioplila extract of the invention can not only be clearly helpful for the reparation that high fat diet causes hepatic injury, but also right
Disease relevant to hepatic injury shows significantly treatment and/or preventive effect, such as the deterioration of inflammation can be effectively relieved, and improves
Immune-related items physiopathological index, can be used in the various fields such as drug, health care product, has great economy and society
It can be worth.
Detailed description of the invention
Fig. 1 is the small intestine HE coloring pathological section figure of embodiment 2.
Fig. 2 is the liver organization pathological section figure of embodiment 2.
Specific embodiment
In the first aspect of the present invention, a kind of trametes robinioplila extract is provided treating and/or prevent high fat diet and causes hepatic injury
Purposes in related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the second aspect of the present invention, a kind of trametes robinioplila extract is provided in preparation treatment and/or prevention high fat diet and causes liver
Damage the purposes in the preparation of related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the third aspect of the present invention, a kind of preparation for preventing and/or treating high fat diet and cause hepatic injury related disease, institute
Stating preparation includes trametes robinioplila extract.
Preferably, the preparation includes drug, health care product or food.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the fourth aspect of the present invention, a kind of drug for preventing and/or treating high fat diet and cause hepatic injury related disease, packet
Extract containing trametes robinioplila and pharmaceutically acceptable carrier.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
Heretofore described " pharmaceutically acceptable carrier " is being cured it is also understood that for " diluent " or " auxiliary material " etc.
It is known that and being described in such as Remington medical science (Remington ' s Pharmaceutical in medicine field
Sciences), Mack Publishing Company (Mack Publishing Co.) (AR lattice nano (A.R.Gennaro) compile,
1985).These substances are nontoxic for recipient under used dosage and concentration, including such as phosphate, citric acid
The buffers such as salt, acetate and other acylates；The antioxidants such as ascorbic acid；The low molecular weights such as poly arginine
(being less than about 10 residues) peptide；The protein such as seralbumin, gelatin or immunoglobulin；Such as polyvinylpyrrolidone
Equal hydrophilic polymers；The amino acid such as glycine, glutamic acid, aspartic acid or arginine；Monosaccharide, disaccharides and including cellulose
Or derivatives thereof, other carbohydrate of glucose, mannose or dextrin；The chelating agents such as EDTA；Such as mannitol or
The sugar alcohols such as sorbierite；The counter ion counterionsl gegenions such as sodium；And/or such as tween (Tween), Pluronic (Pluronics) or poly- second
The nonionic surface active agent such as glycol；Further including includes sterile water, salt water, glucose, oil (such as corn oil, peanut oil and class
Like object), acid.Further can also include preservative, wetting agent, emulsifier, and the like etc..
The dosage of trametes robinioplila extract or drug of the invention can with specific medication and treated object or
Type and change.The dosage is enough to generate expected beneficial effect, and can be formulated for single dose application or multiple dose
Amount application.
Heretofore described drug can be applied by any approach well known by persons skilled in the art, including oral,
The approach such as intramuscular, intravenous, intradermal, intralesional.Application can be part, surface or whole body.In any given feelings
Most suitable approach depends on many factors, such as property, the process of disease, the seriousness of disease of disease etc. under condition.As
It is preferred that trametes robinioplila extract of the invention or being administered orally comprising the drug including trametes robinioplila extract.
The trametes robinioplila extract of the invention can also be applied together with the second reactive compound/second therapeutic agent, simultaneously,
It applies in succession or parallel.When applying respectively, the trametes robinioplila extract of the invention or the drug comprising trametes robinioplila extract and
Two reactive compounds or second therapeutic agent can be applied with different medicine frequencies or interval.
In the purposes of aforementioned four aspect of the invention or preparation, the trametes robinioplila extract include trametes robinioplila water extract,
Trametes robinioplila alcohol extracting thing, trametes robinioplila alkali extract.
In one embodiment, the trametes robinioplila alcohol extracting thing is trametes robinioplila ethanol extract.
In one embodiment, the trametes robinioplila alkali extract is trametes robinioplila sodium hydroxide extract.
Preferably, in the purposes of aforementioned four aspect of the invention or preparation, the trametes robinioplila extract is preferably following
The trametes robinioplila extract that preparation method is prepared.
In the fifth aspect of the invention, a kind of preparation method of trametes robinioplila extract is provided, comprising: crush trametes robinioplila fructification
Afterwards, it is successively mentioned with alcohol extracting, water, the extraction of sodium hydroxide (NaOH aqueous solution) buck, respectively obtains extraction solution, then respectively through dense
Contracting, freeze-drying, obtain trametes robinioplila alcohol extracting thing, trametes robinioplila water extract, trametes robinioplila alkali extract.
Preferably, a kind of preparation method of trametes robinioplila extract, comprising: after crushing trametes robinioplila fructification, successively with 95% second
Alcohol extracting, water are extracted, 1%-2%NaOH buck extracts, and respectively obtain extraction solution, then concentrated, freeze-drying respectively, are obtained
Trametes robinioplila alcohol extracting thing, trametes robinioplila water extract, trametes robinioplila alkali extract.
Preferably, a kind of preparation method of trametes robinioplila extract, comprising: after crushing trametes robinioplila fructification, using alcohol reflux
It extracts, obtains the first filter residue and alcohol extracting solution, the first filter residue is used into water refluxing extraction solution, the second filter residue is obtained and water mentions
Solution is taken, then the second filter residue is used into 1%-2%NaOH aqueous solution refluxing extraction, third filter residue is obtained and alkali carries takes solution, it will
Alcohol extracting solution, water extraction solution obtain trametes robinioplila alcohol extracting thing, trametes robinioplila water extract by concentration, freeze-drying respectively；By alkali
It extracts solution and obtains trametes robinioplila alkali extract by concentration, alcohol precipitation, freeze-drying.
Preferably, it includes: that the ethyl alcohol that solid-liquid ratio is 1:8-1:15 is added that the alcohol reflux, which extracts, and refluxing extraction 2-4 is small
When, it filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp are freeze-dried to get trametes robinioplila alcohol extracting
Object.
Preferably, the water refluxing extraction includes: the filter residue after taking alcohol extracting, uses solid-liquid ratio for the water of 1:8-1:15, is returned
Stream extracts 1-3 hours, filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp, freeze-drying, i.e.,
Obtain trametes robinioplila water extract.
Preferably, the 1%-2%NaOH buck extract include: learn from else's experience alcohol and water mention after filter residue, use solid-liquid ratio for
The buck of 1:8-1:15, stirring is ultrasonic extraction 1-2 hours lower, filters, and obtains filtrate, and alcohol reagent precipitating is added, precipitates 12-24
Hour, precipitating is collected, through -80 DEG C of freeze overnights, is freeze-dried to get trametes robinioplila alkali carries object.
The 1%-2%NaOH or the 1%-2%NaOH buck refer to that weight percentage is the NaOH water of 1%-2%
Solution.
Preferably, the 1%-2%NaOH is 2%NaOH.
It is further preferred that the alcohol reagent of the precipitating includes ethyl alcohol, methanol.
It is further preferred that described be precipitated as that 4-6 times of the liquid ethyl alcohol measured is added, precipitate 12-24 hours.
In the sixth aspect of the present invention, a kind of trametes robinioplila extract that above-mentioned preparation method is prepared is provided.
Trametes robinioplila (ew'sEaronPagodaTCMLIBee), be On Polyporaceae Chinese scholartree bolt bacterium fructification, alias: Chinese scholartree Xiao,
Chinese scholartree bacterium, Chinese scholartree chicken, Chinese scholartree goose, Chinese scholartree moth, red chicken.Chinese scholartree bolt bacterium Trametes robiniophila Murr.Fructification stockless, cap semicircle
Shape is often in imbricate, and suberin, sepia, dipped beam is sliding, there is a small number of ring grains, and bacterial context yellow-white is savory after doing, wall thickness and light
Whole, aperture yellow-white, polygonal, 5~6 between every 1mm, spore is colourless, smooth,Spore printWhite often has cystidium.It is grown on
On the trunks such as Chinese scholartree and acacia, wingceltis.It is distributed in the ground such as Hebei, Shandong, Shaanxi, Sichuan, Chongqing.Wild resource is rare, Jiangsu etc.
Ground uses solid fermentation method culture Chinese scholartree bolt bacterium with hyoscine.Has the effects that antitumor, enhancing immune function and antiviral.
Heretofore described " imbalance " can be understood as " unbalance ", " disorder " or " disease " etc..
As used herein, term "comprising" or " comprising " refer to that method includes cited element, but are not excluded for other
Element.
Trametes robinioplila of the present invention is the fructification of On Polyporaceae Chinese scholartree bolt bacterium, and principal component is trametes robinioplila proteoglycan,
Hydrolysate (fucose) containing L-fucose, L-arabinose (arabinose), D- xylose (xylose), D-MANNOSE
(mannose), D- galactolipin (galactose), 6 kinds of monosaccharide such as D-Glucose (glucose) and aspartic acid (aspartic
Acid), threonine (threonine), serine (serine), glutamic acid (glutamicacid), proline (proline),
Glycine (glycine), alanine (alanine), cystine (cystine), valine (valine), methionine
(methionine), isoleucine (isoleucine), leucine (leucine), tyrosine (tyrosine), phenylalanine
(phenylalanine), lysine (lysine), histidine (histidine), tryptophan (Typtophane), arginine
(arginine) 18 kinds of amino acid compositions such as.This edible mushroom value is high.
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1: the preparation of trametes robinioplila extract, steps are as follows:
(1) trametes robinioplila fructification is crushed, uses solid-liquid ratio for the ethyl alcohol of 1:8-1:15, refluxing extraction 2-4 hours, filtered,
The thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp, enter freeze drier it is dry to get
Trametes robinioplila alcohol extracting thing.
(2) the above-mentioned filter residue after alcohol extracting is taken, uses solid-liquid ratio for the water of 1:8-1:15, refluxing extraction 1-3 hours, is taken out
Filter, the thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp enter freeze drier drying,
Up to trametes robinioplila water extract.
(3) the above-mentioned filter residue after alcohol and water mentions is taken, uses solid-liquid ratio for the 2%NaOH aqueous solution of 1:8-1:15, is stirred
Lower ultrasonic extraction 1-2 hours filters, and obtains filtrate, and debita spissitudo ethyl alcohol is added, and precipitates 12-24 hours；Precipitating is collected, through -80
It is dry to get trametes robinioplila buck extract to enter freeze drier for DEG C freeze overnight.
Obtained trametes robinioplila alcohol extracting thing, trametes robinioplila water extract, trametes robinioplila buck extract are subjected to determination of polysaccharide, polysaccharide
Assay uses People's Republic of China's agricultural industry criteria NY/T 1676-2008 " survey of Thick many candies content in edible mushroom
It is fixed " method；Protein content determination uses Kjeldahl's method.
Table 1 is the Thick many candies content measured in trametes robinioplila extract and protein content.
Thick many candies and protein content in 1 trametes robinioplila extract of table
Embodiment 2: improvement result of the trametes robinioplila extract to high fat diet obesity-induced mice hepatic injury
1, reagent:
Total cholesterol (T-CHO) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Triglycerides (TG) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Low density lipoprotein cholesterol (LDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
High-density lipoprotein cholesterol (HDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Alanine aminotransferase (ALT) testing cassete, total protein (TP) testing cassete, albumin (ALB) testing cassete and ball egg
White (GLB) testing cassete is all purchased from Nanjing and builds up Bioengineering Research Institute.
Female C57 mouse 30, is purchased from Guangdong Province's medical experiment animal by SPF grades, weight 12-14g, age in days 28-30d
Center, credit number are SCXK (Guangdong) 2013-0002.
Rats and mice maintains feed: Guangdong Medical Lab Animal Center, credit number SCXK (Guangdong) 2013-0002.
High cholesterol diet formula high in fat is as shown in table 2.
The main component and energy of the high cholesterol diet high in fat of table 2 are constituted
Trametes robinioplila extract is prepared by experimental example 1 of the present invention.
2, method
The preparation of high fat diet obesity-induced mice model: animal subject is raised in the zoopery of Institute of Micro-biology, Guangdong Province
The heart, raising temperature and humidity: 23 ± 1 DEG C, 55 ± 10%, using 12h intermittent illumination round the clock；Receptacle condition remains steady
It is fixed, with the reliability of guarantee test result.Mouse ad lib drinking-water.It is primary that period is checked daily animal, does not find unhealthy
Animal, take whole healthy mices to be tested.50 C57 mouse are randomly divided into normal group, model after adaptive feeding 7d
Group, trametes robinioplila alcohol extracting group, trametes robinioplila water propose group and trametes robinioplila alkali carries group, and every group 10.Normal group is fed with normal diet, model group and is given
Medicine group is fed with high cholesterol diet high in fat, continuous to feed 2 months.
After modeling success, start to be administered, administration group gives trametes robinioplila water extract, trametes robinioplila alcohol extracting thing and trametes robinioplila alkali carries respectively
Take object stomach-filling, drug concentration 6mg/ml (180g extract is dissolved in 30ml water), 3mg/ pcs/day of dosage, dosage 0.5ml/ only/
It, successive administration 35 days, while normal group mouse continues to be fed with normal diet, administration group and model group mouse use common feeding instead
Material is fed.
3, result is observed
The appearance posture (hair, color, shape) and dynamic of daily observation animal simultaneously make respective record, measure the weight of animals every 7d
1 time.
Blood: after being discontinued for 24 hours, animal materials testing index.Acquire blood sample, pluck eyeball and take blood, blood plasma through 3000RPM,
Serum is collected in 10min centrifugation twice, and it is horizontal to measure T-CHO, TG, LDL-C, HDL-C, TP, ALB, GLB, ALT in serum.
Tissue: it plucks after eyeball takes blood, puts to death and dissect mouse, take mouse small intestine and liver organization, be fixed on 4% poly first
In aldehyde.Small intestine and liver organization are made into paraffin section, and small intestine is dyed using Hematoxylin-eosin (HE) method, liver organization
Hematoxylin-eosin (HE), masson, oil red O (ORS) is respectively adopted, carries out pathology section examination.
4, data processing
All data are with mean ± standard deviationIt indicates.The comparison of mean uses one-way analysis of variance between multiple groups
(One-Way ANOVA), mean compares two-by-two between group, and LSD method is used when variance is neat；Dunnett ' s T3 is used when heterogeneity of variance
Method.It is completed by SPSS software, α=0.05.
It has been observed that the fur of the animal of administration group is more smoother than the fur of model group.
The variation of 3 mouse weight of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
The changes of weight of each group is as shown in table 3.
From table 3 it can be seen that the fur of the animal by treatment is more smoother than the fur of model group.At the end of experiment, just
Average weight significant difference (P < 0.05) between normal group and model group, average weight is about when starting for model group mouse
65.45g, average weight is 72.76g at the end of experiment, illustrates high fat diet obesity-induced mice model modeling success；With mould
Type group is compared, and 3 administration group average mice body weights are remarkably decreased (P < 0.001) (table 3).This shows: suitable to obesity mice stomach-filling
The trametes robinioplila extract of amount can significantly slow weight gain.
The four items of blood lipid tests testing result of each group is as shown in table 4.
4 mice serum biochemical indicator (mmol/L) of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
From table 4, it can be seen that in model group total cholesterol, triglycerides and ldl-cholesterol ratio normally organize it is aobvious
It writes and increases (P < 0.01 or P < 0.001), high-density lipoprotein cholesterol significantly reduces (P < 0.001) than normal group；With model group
It compares, under triglycerides (T-CHO), total cholesterol (TG), low density lipoprotein cholesterol (LDL-C) are horizontal significantly in administration group
It drops (P < 0.05 or P < 0.01 or P < 0.001), the horizontal significant raising (P < 0.05) of high-density lipoprotein cholesterol (HDL-C).This
Show: total cholesterol in serum, triglycerides and low can be significantly reduced to the suitable trametes robinioplila extract of obesity mice stomach-filling
Density lipoprotein levels.
The results are shown in Table 5 for the liver functional testing of each group.
5 liver functional testing result of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
Serum alt and ALB/GLB can reflect liver function damage degree, when liver cell is damaged, ALB synthesis decline, and ball egg
White balance imbalance, causes ALB/GLB to decline.As can be seen from Table 5, compared with normal group, ALT is significant in model group mice serum
It increases (P < 0.05), shows that high fat diet causes hepatic injury modeling success；Compared with model group, in administration group ALT significantly reduce (P <
0.05), ALB and ALB/GLB significantly increases (P < 0.05).Show that trametes robinioplila extract can improve hepatic injury.
Small intestine HE coloring pathological section result as shown in Figure 1, it can be seen from figure 1 that normally group small intestine's villus it is long and
Completely, model group intestinal villus falls off seriously, illustrates that high fat diet can cause to damage to the enteron aisle of mouse, illustrates that model is success
's.Compared with model group, after drug-treated, administration group can alleviate damage, promote the reparation of enteron aisle.
Liver organization pathological section result is as shown in Fig. 2, figure it is seen that HE coloration result shows normal group mouse
Liver cell structure is normal, and model group mouse liver cell volume increases, and occurs many circles not of uniform size in hepatocyte cell matter
Blown-out shot pushes nucleus to side, locally there is inflammatory cell infiltration phenomenon；Compared with model group, administration group mouse liver cell rouge
Fat vacuole quantity significantly reduces, and inflammatory cell infiltration phenomenon is improved；Masson is dyed and ORS coloration result is shown respectively,
Compared with model group, administration group mouse liver cell structure is obviously improved, and tends to normal group.
Inventor also found that high fat diet obesity-induced mice model makes its intestinal microflora that significant changes occur,
Trametes robinioplila extract is added in high cholesterol diet high in fat and is clearly helpful for dominant microflora structure recovery in enteron aisle, and it is more to facilitate flora
Sample is effectively improved intestinal bacilli illness, repairs intestinal mucosa, effectively inhibits the deterioration for alleviating inflammation, improves immune-related
Every physiopathological index.It has been recognised by the inventors that trametes robinioplila extract is based on this so as to improve caused by high fat diet
Hepatic injury can be used in the various fields such as drug, health care product, have great economy and society value.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease.
2. purposes according to claim 1, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
3. the preparation of a kind of prevention and/or treatment hepatic injury related disease, which is characterized in that the preparation is extracted comprising trametes robinioplila
Object.
4. preparation according to claim 4, which is characterized in that the preparation includes drug, health care product or food.
5. preparation according to claim 4, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
6. the drug that a kind of prevention and/or treatment high fat diet cause hepatic injury related disease, which is characterized in that extracted comprising trametes robinioplila
Object and pharmaceutically acceptable carrier.
7. drug according to claim 6, which is characterized in that the trametes robinioplila extract includes trametes robinioplila water extract, trametes robinioplila
Alcohol extracting thing, trametes robinioplila alkali extract.
8. a kind of preparation method of trametes robinioplila extract characterized by comprising by trametes robinioplila fructification crush after, successively with alcohol extracting,
Water mentions, NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freeze-drying respectively, obtains trametes robinioplila alcohol extracting thing, Chinese scholartree
Ear water extract, trametes robinioplila alkali extract.
9. preparation method according to claim 8 characterized by comprising after crushing trametes robinioplila fructification, successively use
95% ethyl alcohol extracts, water extracts, 1%-2%NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freezing is done respectively
It is dry, obtain trametes robinioplila alcohol extracting thing, trametes robinioplila water extract, trametes robinioplila alkali extract.
10. preparation method according to claim 8 characterized by comprising after crushing trametes robinioplila fructification, using second
Alcohol reflux extracts, and obtains the first filter residue and alcohol extracting solution, and the first filter residue is used water refluxing extraction solution, obtains the second filter residue
And water extraction solution, then the second filter residue is used into 1%-2%NaOH aqueous solution refluxing extraction, obtain third filter residue and alkali carries take it is molten
Alcohol extracting solution, water extraction solution are obtained trametes robinioplila alcohol extracting thing, trametes robinioplila water extract by concentration, freeze-drying respectively by liquid；
Solution is taken to obtain trametes robinioplila alkali extract by concentration, alcohol precipitation, freeze-drying alkali carries.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910233587.3A CN109771455A (en) | 2019-03-26 | 2019-03-26 | Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910233587.3A CN109771455A (en) | 2019-03-26 | 2019-03-26 | Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109771455A true CN109771455A (en) | 2019-05-21 |
Family
ID=66490860
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910233587.3A Pending CN109771455A (en) | 2019-03-26 | 2019-03-26 | Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109771455A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105384843A (en) * | 2015-12-17 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Method for extracting water soluble beta-glucan from inonotus obliquus sporophore |
| CN106573028A (en) * | 2014-06-10 | 2017-04-19 | 摩诗玫德株式会社 | Composition for preventing and treating non-alcoholic fatty liver diseases |
| CN108379286A (en) * | 2018-03-14 | 2018-08-10 | 广东省微生物研究所（广东省微生物分析检测中心） | Mushroom dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease |
| CN108670987A (en) * | 2018-07-27 | 2018-10-19 | 广东省微生物研究所 | The preparation method of Inonotus obliquus extract and its in the purposes for preparing anti-bleb preparation |
-
2019
- 2019-03-26 CN CN201910233587.3A patent/CN109771455A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106573028A (en) * | 2014-06-10 | 2017-04-19 | 摩诗玫德株式会社 | Composition for preventing and treating non-alcoholic fatty liver diseases |
| CN105384843A (en) * | 2015-12-17 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Method for extracting water soluble beta-glucan from inonotus obliquus sporophore |
| CN108379286A (en) * | 2018-03-14 | 2018-08-10 | 广东省微生物研究所（广东省微生物分析检测中心） | Mushroom dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease |
| CN108670987A (en) * | 2018-07-27 | 2018-10-19 | 广东省微生物研究所 | The preparation method of Inonotus obliquus extract and its in the purposes for preparing anti-bleb preparation |
Non-Patent Citations (2)
| Title |
|---|
| 刘凯等: "大鼠高胆固醇血症快速造型法初筛降脂中草药", 《徐州医学院学报》 * |
| 刘荣等: "黑木耳多糖降血脂作用的小鼠实验分析", 《食品研究与开发》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3763830A1 (en) | Nanovesicles derived from lactobacillus sp. bacteria, and use thereof | |
| US11529377B2 (en) | Nano-vesicles derived from genus Sphingomonas bacteria and use thereof | |
| US6964785B2 (en) | Herbal composition for improving anticancer activity, immune response and hematopoiesis of the body, and protecting the body from oxidative damage, and the method of preparing the same | |
| CA2871478C (en) | Pleuropterus multiflorus extract and dipsacus asperoides extract for stimulating the secretion of insulin-like growth factor and promoting bone structure growth, and method for preparing same | |
| CN109771457A (en) | Purposes of the russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease | |
| KR101487541B1 (en) | Analytical method of ginseng polysaccharide | |
| CN109771455A (en) | Purposes of the trametes robinioplila extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease | |
| CN109758485A (en) | Purposes of the russule extract in the preparation of preparation treatment and/or prevention intestinal bacilli illness disease and related disease | |
| DE10392697B4 (en) | Extract of peptide-linked glucans and peptide-linked clucans with detectable oral absorbability from Coriolus versicolor and their purification methods and uses | |
| CN109771454A (en) | Purposes of the birch young pilose antler extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease | |
| CN109771453A (en) | Purposes of the birch young pilose antler extract in the preparation of preparation treatment and/or prevention intestinal bacilli illness disease and related disease | |
| CN108379284A (en) | Purposes of the black tiger palm dietary fiber extract in preparing treatment and/or preventing intestinal bacilli illness relevant disease preparation | |
| CN106924297A (en) | A kind of Lachnum intracellular melanin as acute liver damage medicine purposes | |
| US20210002707A1 (en) | Nanovesicles derived from blautia bacteria and use thereof | |
| JP2001064195A (en) | Collection of physiologically active substance from mushrooms and/or yeasts, and the physiologically active substance | |
| KR20080093196A (en) | Exopolysaccharides for type 2 diabetes therapy from phellinus baumii mycellium and a preparation method thereof | |
| JP2004059558A (en) | Method for collecting physiologically active substance from fungus, preparation of physiologically active substance of the same and use of the same | |
| CN109771456A (en) | Purposes of the trametes robinioplila extract in the preparation of preparation treatment and/or prevention intestinal bacilli illness disease and related disease | |
| CN110327379A (en) | A kind of activation fatty acid-g protein coupled receptor access composition | |
| JP7168196B2 (en) | Intestinal barrier function improver | |
| CN115969889A (en) | Application of cordyceps militaris sporocarp extract in preparing medicine for improving blue light injury resistance effect | |
| KR20100070154A (en) | Anti-diabetic composition comprising essentially of chlorella hot extract | |
| CN107073076A (en) | Immune activation agent, cellular immunity activator and T cell multiplication agent | |
| EP3896175A1 (en) | Nano-vesicle derived from corynebacterium sp. bacteria and use thereof | |
| KR20140006750A (en) | Extract from salted and fermented shrimp, manufacturing method of the same and composition containing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190521 |
|
| RJ01 | Rejection of invention patent application after publication |