CN109765165A - A kind of magnetic bead suitable for cell sample flow cytometer detection - Google Patents
A kind of magnetic bead suitable for cell sample flow cytometer detection Download PDFInfo
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- CN109765165A CN109765165A CN201910131207.5A CN201910131207A CN109765165A CN 109765165 A CN109765165 A CN 109765165A CN 201910131207 A CN201910131207 A CN 201910131207A CN 109765165 A CN109765165 A CN 109765165A
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- magnetic bead
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- flow cytometer
- streaming
- combination
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Abstract
The present invention relates to cell sample flow cytometer detection technical fields, in particular a kind of magnetic bead suitable for cell sample flow cytometer detection, including streaming instrument main body, magnetic bead adsorbs combination and cell adsorbs combination, the streaming instrument body side is equipped with streaming loading pipeline, the streaming loading pipeline is equipped with testing tube far from one end of streaming instrument main body, test sample solutions are equipped in the testing tube, it is equipped with magnetic bead absorption combination in the test sample solutions and cell adsorbs combination, the magnetic bead absorption combination includes magnetic bead surfaces antigen, fluorescence, magnetic bead, binding site and fluorescence antibody, the cell absorption combination includes fluorescence antibody, target cell and targeted cell surface antigen, ferromagnetism sample placed seat is equipped with outside the testing tube, cooperate automated system in the present invention, carry out extensive fluorescence antibody flow cytometer detection, it is convenient for It is matched with modules such as automatic liquid-feedings in automated system, convenient for scale detection operation.
Description
Technical field
It is specially a kind of suitable for cell sample flow cytometer detection the present invention relates to cell sample flow cytometer detection technical field
Magnetic bead.
Background technique
Cell therapy and application have stepped into the scale application stage, and with the demand of large-scale production, batch is efficiently
The cell quality of rate detects while becoming necessary, and flow cytometer detection is the main means during cell detection, cooperates fluorescence antibody
Multiple types marker can be detected, but effectively in order to obtain higher Detection accuracy, before carrying out actual detection operation, needed
Multiple centrifugation, piping and druming resuspension are carried out, to remove the remaining fluorescence antibody not combined effectively with cell, since it is desired that multiple
Centrifugation, piping and druming are resuspended and cooperate the extraction of target liq, and existing manual operations efficiency is difficult to meet the needs of detecting on a large scale, such as
The work such as complicated centrifugation, piping and druming are completed in automation equipment, can also seriously affect the execution efficiency of system, and significantly mention
The complexity of high system needs a kind of mode being simple and efficient, and when carrying out streaming fluorescence detection, efficiently removes sample solution
In remaining fluorescence antibody, the noise in removal process, to obtain accurate detection result, it is therefore desirable to which one kind is suitable for thin
The magnetic bead of born of the same parents' sample flow cytometer detection makes improvement to the above problem.
Summary of the invention
The purpose of the present invention is to provide a kind of magnetic beads suitable for cell sample flow cytometer detection, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the invention provides the following technical scheme:
A kind of magnetic bead suitable for cell sample flow cytometer detection, including streaming instrument main body, magnetic bead absorption combination and thin
Born of the same parents adsorb combination, and the streaming instrument body side is equipped with streaming loading pipeline, and the streaming loading pipeline is far from streaming instrument master
One end of body is equipped with testing tube, and test sample solutions are equipped in the testing tube, magnetic bead is equipped in the test sample solutions and inhales
Attached combination and cell adsorb combination, and the magnetic bead absorption combination includes magnetic bead surfaces antigen, fluorescence, magnetic bead, binding site
And fluorescence antibody, and the magnetic bead surfaces are equipped with magnetic bead surfaces antigen, the magnetic bead surfaces antigen passes through far from the side of magnetic bead
Binding site is fixedly connected with fluorescence antibody, and the fluorescence antibody is equipped with fluorescence, the cell far from one end of magnetic bead surfaces antigen
Adsorbing combination includes fluorescence antibody, target cell and targeted cell surface antigen, and the targeted cell surface is equipped with target
Cell surface antigen, the targeted cell surface antigen are fixedly connected with fluorescence antibody, the survey far from one end of target cell
Ferromagnetism sample placed seat is equipped with outside test tube.
Preferably, the fluorescence antibody and magnetic bead surfaces antigen are combined by hatching.
Preferably, the fluorescence antibody and targeted cell surface antigen are combined by hatching.
Preferably, the magnetic bead absorption combination and ferromagnetism sample placed seat pass through magnetic-adsorption.
Preferably, using excessive investment, the magnetic bead uses micron order magnetic bead for the magnetic bead metering.
Preferably, one end of the streaming loading pipeline is placed in testing tube inner lower.
Compared with prior art, the beneficial effects of the present invention are:
1, in the present invention, simplify the centrifugation in optimization streaming fluorescence detection, blow and beat resuspension, the behaviour that target solution is extracted
Make, simplifies many and diverse work such as more wheel centrifugations, washing in traditional operation.
2, in the present invention, cooperate automated system, carry out extensive fluorescence antibody flow cytometer detection, be convenient for and automated system
In the modules such as automatic liquid-feeding matched, convenient for scale detection operation.
3, in the present invention, cooperate mass cell flow cytometer detection, efficiently remove extra fluorescence antibody bring noise jamming.
Detailed description of the invention
Fig. 1 is a kind of overall structure figure of the magnetic bead suitable for cell sample flow cytometer detection proposed by the present invention；
Fig. 2 is that a kind of magnetic bead of magnetic bead suitable for cell sample flow cytometer detection proposed by the present invention adsorbs combination structure
Figure；
Fig. 3 is that a kind of cell of magnetic bead suitable for cell sample flow cytometer detection proposed by the present invention adsorbs combination structure
Figure.
In figure: 1- streaming instrument main body, 2- streaming loading pipeline, 3- testing tube, 4- test sample solutions, 5- magnetic bead absorption knot
Fit, 6- cell absorption combination, 7- ferromagnetism sample placed seat, 8- magnetic bead surfaces antigen, 9- fluorescence, 10- magnetic bead, 11- knot
Chalaza, 12- fluorescence antibody, 13- target cell, 14- targeted cell surface antigen.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution:
A kind of magnetic bead suitable for cell sample flow cytometer detection, including streaming instrument main body 1, magnetic bead absorption combination 5 and
Cell adsorbs combination 6, and 1 side of streaming instrument main body is equipped with streaming loading pipeline 2, and streaming loading pipeline 2 is far from streaming instrument main body 1
One end be equipped with testing tube 3, one end of streaming loading pipeline 2 is placed in 3 inner lower of testing tube, and test specimens are equipped in testing tube 3
This solution 4, test sample solutions 4 are interior to be equipped with magnetic bead absorption combination 5 and cell absorption combination 6, and magnetic bead adsorbs the packet of combination 5
Antigen containing magnetic bead surfaces 8, fluorescence 9, magnetic bead 10, binding site 11 and fluorescence antibody 12, the metering of magnetic bead 10 is using excessive investment, magnetic bead
Using micron order magnetic bead, and 10 surface of magnetic bead is equipped with magnetic bead surfaces antigen 8, and side of the magnetic bead surfaces antigen 8 far from magnetic bead 10 is logical
It crosses binding site 11 and is fixedly connected with fluorescence antibody 12, fluorescence antibody 12 and magnetic bead surfaces antigen 8 by hatching combination, fluorescence antibody
12 one end far from magnetic bead surfaces antigen 8 are equipped with fluorescence 9, and it includes fluorescence antibody 12,13 and of target cell that cell, which adsorbs combination 6,
Targeted cell surface antigen 14, and 13 surface of target cell is equipped with targeted cell surface antigen 14, fluorescence antibody 12 and target are thin
Cellular surface antigen 14 is combined by hatching, and targeted cell surface antigen 14 is fixedly connected with fluorescence far from one end of target cell 13
Antibody 12 is equipped with ferromagnetism sample placed seat 7 outside testing tube 3, and magnetic bead adsorbs combination 5 and ferromagnetism sample placed seat 7 is logical
Cross magnetic-adsorption.
Workflow of the present invention: basic process for using:
Step 1: fluorescence antibody 12 is added in test sample solutions 4；
Step 2: be added fluorescence antibody 12 test sample solutions 4 hatched, ensure targeted cell surface antigen 14 with
Fluorescence antibody 12 effectively combines；
Step 3: excessive magnetic bead 10 is added；
Hatch step 4: carrying out magnetic bead 10；
Step 5: testing tube 3 is placed into the loading position of ferromagnetism sample placed seat 7；
Step 6: streaming instrument main body 1 carries out loading test.
Magnetic bead surfaces antigen 8, simulated target cell surface antigen 14 and extra fluorescence are combined on micron-sized magnetic bead 10
Antibody 12 combines, and in streaming loading, the cell that the combination of targeted cell surface antigen 14 and fluorescence antibody 12 is formed adsorbs knot
Zoarium 6 is detected normal through the liquid channel system that streaming loading pipeline 2 enters streaming instrument main body 1, magnetic bead surfaces antigen 8 and more
Remaining fluorescence antibody 12 combines the magnetic bead formed absorption combination 5 due to the effect of magnetic force, stays on the chamber wall of testing tube 3,
The liquid channel system for not entering streaming instrument main body 1, after having extracted cell absorption combination 6, testing tube 3 can be discarded, and no longer need
Any follow-up work is wanted, is such as cleaned, simplifies the centrifugation in optimization streaming fluorescence detection, piping and druming is resuspended, target solution mentions
The operation taken simplifies many and diverse work such as more wheel centrifugations, washing in traditional operation, cooperates automated system, carries out extensive glimmering
Photoactivated antibody flow cytometer detection, convenient for being matched with modules such as automatic liquid-feedings in automated system, convenient for scale detection operation,
Cooperate mass cell flow cytometer detection, efficiently removes extra fluorescence antibody bring noise jamming.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of magnetic bead suitable for cell sample flow cytometer detection, including streaming instrument main body (1), magnetic bead absorption combination (5) with
And cell absorption combination (6), it is characterised in that: the streaming instrument main body (1) side is equipped with streaming loading pipeline (2), described
Streaming loading pipeline (2) is equipped with testing tube (3) far from one end of streaming instrument main body (1), and test specimens are equipped in the testing tube (3)
This solution (4), the interior magnetic bead that is equipped with of the test sample solutions (4) adsorbs combination (5) and cell absorption combination (6), described
It includes magnetic bead surfaces antigen (8), fluorescence (9), magnetic bead (10), binding site (11) and fluorescence antibody that magnetic bead, which adsorbs combination (5),
(12), and the magnetic bead (10) surface is equipped with magnetic bead surfaces antigen (8), and the magnetic bead surfaces antigen (8) is far from magnetic bead (10)
Side is fixedly connected with fluorescence antibody (12) by binding site (11), and the fluorescence antibody (12) is far from magnetic bead surfaces antigen (8)
One end be equipped with fluorescence (9), cell absorption combination (6) includes that fluorescence antibody (12), target cell (13) and target are thin
Cellular surface antigen (14), and the target cell (13) surface is equipped with targeted cell surface antigen (14), the target cell table
Face antigen (14) is fixedly connected with fluorescence antibody (12) far from the one end of target cell (13), is equipped with outside the testing tube (3)
Ferromagnetism sample placed seat (7).
2. a kind of magnetic bead suitable for cell sample flow cytometer detection according to claim 1, it is characterised in that: the fluorescence
Antibody (12) and magnetic bead surfaces antigen (8) are combined by hatching.
3. a kind of magnetic bead suitable for cell sample flow cytometer detection according to claim 1, it is characterised in that: the fluorescence
Antibody (12) and targeted cell surface antigen (14) are combined by hatching.
4. a kind of magnetic bead suitable for cell sample flow cytometer detection according to claim 1, it is characterised in that: the magnetic bead
Absorption combination (5) and ferromagnetism sample placed seat (7) pass through magnetic-adsorption.
5. a kind of magnetic bead suitable for cell sample flow cytometer detection according to claim 1, it is characterised in that: the magnetic bead
(10) metering uses micron order magnetic bead using excessive investment, the magnetic bead (10).
6. a kind of magnetic bead suitable for cell sample flow cytometer detection according to claim 1, it is characterised in that: the streaming
One end of loading pipeline (2) is placed in testing tube (3) inner lower.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910131207.5A CN109765165B (en) | 2019-02-22 | 2019-02-22 | Method suitable for flow detection of cell sample |
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| CN201910131207.5A CN109765165B (en) | 2019-02-22 | 2019-02-22 | Method suitable for flow detection of cell sample |
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Citations (7)
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| US20050059097A1 (en) * | 1998-03-17 | 2005-03-17 | The University Of Kentucky Research Foundation | Quantitative binding assays using green fluorescent protein as a label |
| CN103308679A (en) * | 2013-06-03 | 2013-09-18 | 江苏大学 | Method for sensing bacteria through utilizing nanometer fluorescent carbon spot immunolabelling marks |
| CN103869060A (en) * | 2014-03-07 | 2014-06-18 | 复旦大学附属中山医院 | Circulating tumor stem cell detection kit based on magnetic beads and microfluidic chip |
| CN106053786A (en) * | 2016-06-01 | 2016-10-26 | 章健 | Fluorescence detection method for time resolution fluorescence marker and device of method |
| CN108007989A (en) * | 2016-10-28 | 2018-05-08 | 中国科学院烟台海岸带研究所 | Based on detection method of the type polypeptide for identification molecule of splitting |
| CN109164255A (en) * | 2018-10-12 | 2019-01-08 | 南京工业大学 | A kind of method of super sensitivity detection small-molecule substance |
| CN109298177A (en) * | 2018-11-06 | 2019-02-01 | 江苏美克医学技术有限公司 | Time-resolved fluorescence immunoassay method based on Magneto separate |
-
2019
- 2019-02-22 CN CN201910131207.5A patent/CN109765165B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050059097A1 (en) * | 1998-03-17 | 2005-03-17 | The University Of Kentucky Research Foundation | Quantitative binding assays using green fluorescent protein as a label |
| CN103308679A (en) * | 2013-06-03 | 2013-09-18 | 江苏大学 | Method for sensing bacteria through utilizing nanometer fluorescent carbon spot immunolabelling marks |
| CN103869060A (en) * | 2014-03-07 | 2014-06-18 | 复旦大学附属中山医院 | Circulating tumor stem cell detection kit based on magnetic beads and microfluidic chip |
| CN106053786A (en) * | 2016-06-01 | 2016-10-26 | 章健 | Fluorescence detection method for time resolution fluorescence marker and device of method |
| CN108007989A (en) * | 2016-10-28 | 2018-05-08 | 中国科学院烟台海岸带研究所 | Based on detection method of the type polypeptide for identification molecule of splitting |
| CN109164255A (en) * | 2018-10-12 | 2019-01-08 | 南京工业大学 | A kind of method of super sensitivity detection small-molecule substance |
| CN109298177A (en) * | 2018-11-06 | 2019-02-01 | 江苏美克医学技术有限公司 | Time-resolved fluorescence immunoassay method based on Magneto separate |
Non-Patent Citations (1)
| Title |
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| 车玉兰: "基于荧光标记的致病菌快速检测及细菌与细胞相互作用研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
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