CN109762863A - A kind of preparation method of krill active oligopeptide, the krill active oligopeptide of this method preparation and application - Google Patents
A kind of preparation method of krill active oligopeptide, the krill active oligopeptide of this method preparation and application Download PDFInfo
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Abstract
The krill active oligopeptide prepared the invention discloses a kind of preparation method of krill active oligopeptide, this method and application, belong to that marine organisms are high-valued, field of deep.The preparation method of krill active oligopeptide of the invention, the pretreatment including degreasing krill powder raw material；Protease hydrolyzed is added under optimum conditions；Enzyme deactivation after enzymatic hydrolysis；It stands cold filtration and obtains supernatant；Active carbon is added to decolourize；Filtered destainer is dried, krill active oligopeptide is obtained.Krill active oligopeptide homogeneity produced by the present invention is high, color brilliant white, soluble easily in water and clear.
Description
Technical field
A kind of high-valued, field of deep that the invention belongs to marine organisms, and in particular to system of krill active oligopeptide
Preparation Method, the krill active oligopeptide of this method preparation and application.
Background technique
Krill (Euphausia superba) is a kind of high crustacean plankton, and biological classification is Euphausiacea
(Euphausiacea), krill section (Euphausiidae), krill category (Euphausia), mainly live on the south 50 degree of south latitude
Shallow-layer waters, long body is about 4-6cm, and whole body is relatively transparent, is breathed with the cheek, and the appendage by body two sides moves, in optic stalk base portion, head
The second to the 7th pereiopoda two sides of chest, sternite have globe-type luminescence device, and the light of yellow green, service life 5-7 can be issued every 2-3 seconds
Year, prey on phytoplankton and zooplankter.It is that reserves are most abundant in the world at present and the living resources of non-large-scale development,
The biomass of the storage of conservative estimation at present is 6.5 × 108~10 × 108Ton, stock number have overwhelming dominance.
The development of resources of krill starts from the last century 60's Mo, has arrived the mid-1970s, gradually realizes business
Change fishing.In view of the fragility of Antarctic ecosystems, the South Pole ocean conservation of living resources committee (CCAMLR) thinks, not
Under conditions of influencing Antarctic ecosystems and krill resource regeneration, annual maximum suggestion amount of fishing is 4 × 106~6 ×
106Ton.China starts late to the research of krill, investigates since the 1990s krill resource
And research, formally start commercialization fishing after 2009, Chinese Antarctic krill year amount of fishing is 3 × 10 at present4~5 × 104Ton.
Antarctic krill is known as " marine protein resources bank ", and up to 18 kinds of amino acid are contained in hydrolysate, wherein
Including whole 8 kinds of amino acid (TAA) necessary to human body: methionine, tryptophan, lysine, valine, isoleucine, bright
Propylhomoserin, phenylalanine, threonine, content account for the 45.28% of Antarctic krill total amino acid content, wherein krill
Lysine content is high, than tuna, brave line shrimp and beef will be high.Remaining 10 kinds are nonessential amino acid (NEAA), completely
Meet the amino acid compositional model for the ideal protein that FAO/WHO the is indicated: (south EAA:NEAA >=60%, EAA:TAA ≈ 40%
The amino acid of pole krill forms, this two groups ratio is 45% respectively, 82%).Traditional degreasing euphausia superba powder is South Pole phosphorus
It is obtained after shrimp med oil and grease extracting, protein content is between 65-70%.Because containing some polysaccharide such as cellulose, chitin
Substance and ash content, mostly as aquatic feeds.The advantage of krill albumen powder is not fully played, and product price is not high.
Oligopeptides (Oligo-peptide) is the protein precursor as made of 2-12 combination of amino acids, or by albumen
Matter is degraded to the aleuroplast of 2-12 amino acid composition, also referred to as small peptide, small peptide, oligopeptide, and molecular weight is less than 1000Da,
The final principal mode of protein digestion products, have the function of multiple biological activities and, such as blood pressure lowering is hypoglycemic, anti-oxidant,
It is antitumor, immunity etc. is improved, the multiple fields such as medicine, functional food, cosmetics have been widely used in.Krill peptide system at present
The standby enzymatic isolation method used is mostly easily enriched with salt ash content.Domestic 106381322 A of patent CN prepares south by raw material of euphausia superba powder
Pole krill active peptide, by nanofiltration device desalination, domestic 104263787 B of patent CN uses ion exchange column for operating unit
Mobile bed apparatus carry out deliming, though its significant effect is at high cost, operate it is relative complex.
Summary of the invention
In view of the above drawbacks of the prior art and problem, the object of the present invention is to provide a kind of active krill oligopeptides
Simple preparation method is a kind of higher value application method of degreasing euphausia superba powder, and the preparation method is easy to operate, technique is simple
It is single, suitable for industrial production, gained krill active oligopeptide homogeneity is high, ash content is low, content of beary metal is low, salt content is low,
And equal molecular weight≤400Da is put down, middle-molecular-weihydroxyethyl is 80% or more in 1000Da oligopeptides proportion below, and yield is up to
85% or more, 95% or more the extraction rate of protein of degreasing krill meal.
In order to achieve the above object, the present invention provides the following technical scheme that
A kind of preparation method of krill active oligopeptide, the pretreatment including degreasing krill powder raw material；In suitable condition
Lower addition protease hydrolyzed；Enzyme deactivation after enzymatic hydrolysis；It stands cold filtration and obtains supernatant；Active carbon is added to decolourize；Incited somebody to action
Destainer after filter is dried, and obtains krill active oligopeptide, the specific steps are as follows:
(1) pretreatment of raw material: degreasing krill meal and water are uniformly mixed according to 1:10-1:100 (w/w) ratio, after washing,
Collect solid；
(2) it digests: pretreated degreasing krill meal (by dry weight) is mixed with the ratio of water 1:2-1:50 (w/w),
Protease is added into mixed liquor to be digested；
(3) enzyme deactivation: after enzymatic hydrolysis, 80-100 DEG C, enzyme deactivation 5-30min；
(4) it filters: being filtered after the solution cooling after enzyme deactivation, separating solid residue and filtrate, the filtrate is
The krill albumen peptide solution digested from degreasing krill meal, color are dark brown；
(5) it decolourizes: carrying out decolorization in the active carbon of above-mentioned filtrate addition 0.2%-5% (w/w)；
(6) it filters: being filtered to remove active carbon, obtain destainer, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned destainer is dried to obtain krill active oligopeptide.
On the basis of above scheme, the amount ratio of degreasing krill meal and water is 1:50 in step (1), divides 2~6 times and washes
It washs.
On the basis of above scheme, protease in step (2) be alkali protease, flavor protease, pepsin,
At least one of compound protease.
On the basis of above scheme, enzymatic hydrolysis is 10-70 DEG C in step (2), keeps the temperature 2-12h.
On the basis of above scheme, filter type is vacuum filtration, vacuum degree: 0.08-- 0.01MPa in step (4)；
The suction filtration time is 5-15min.
On the basis of above scheme, the decolorization in step (5) is 30 DEG C, 40~50min.
On the basis of above scheme, filter type is vacuum filtration, vacuum degree: 0.08-- 0.01MPa in step (6)；
Filter the time: 10-20min.
On the basis of above scheme, the drying in step (7) is spray drying or freeze-drying.
The krill active oligopeptide of above method preparation.
On the basis of above scheme, krill active oligopeptide the answering in medicine, functional food, cosmetics
With.
The alkali protease selected in the method for the present invention for preparing krill active oligopeptide faces upward splendid life purchased from Henan
Change Engineering Co., Ltd, Pangbo Bioengineering Co Ltd, Nanning, Hebei Ge Beida Biotechnology Co., Ltd；Enzymolysis time exists
2-12h effect is best, activated carbon addition 0.2%-5%, and decolorizing effect is best.It does not need to adjust the behaviour such as pH value before enzymatic hydrolysis
Make step, simplify operation sequence, keeps operating procedure simpler easy, it is time-consuming shorter, it avoids and is adjusted using sodium hydroxide
The pH value of enzymolysis liquid and introduce more sodium salts, influence the taste of final polypeptide products, while the pretreatment of raw material effectively takes off
In addition in product ash content and reduce product salt content, avoid krill peptide purification when desalination, deliming and etc. and reduce
The degreasing euphausia superba powder hydrolyzed peptide of the dosage of decoloration active carbon, preparation has the various actives such as anti-oxidant, blood pressure lowering, molecule
Measure low, solute effect is good, it is easy absorb, the features such as it is high, sodium content is low, and nutrient is high with potassium calcium content simultaneously, have very
High practical application value.
Beneficial effect possessed by the present invention
(1) present invention in raw material without pretreated ash content be 10-15%, after pretreatment ash content containing amount≤5%, can take off
Except 30-90% salt, make F-Content is 30-80 μ g/g, Na+Han Liang≤80mg/Kg, K+Content is in 150-200mg/Kg, Ca2+≥
3500mg/Kg、Mg2+In 3500-5000mg/Kg.
(2) krill active oligopeptide homogeneity produced by the present invention is high, color brilliant white, soluble easily in water and clear.
(3) 85% or more krill active oligopeptide product recovery rate produced by the present invention, degreasing krill meal protein extraction
95% or more rate, molecular weight are 80% or more in 1000Da oligopeptides proportion below, increase degreasing euphausia superba powder
Utilization rate improves krill value-added content of product.
(4) krill active oligopeptide product quality produced by the present invention is high, yield is high, and preparation method is easy to operate, work
Skill is simply easily achieved industrialization, and economic benefit and social benefit are significant.
Detailed description of the invention
Fig. 1: for process route view of the invention.
Fig. 2: the graph of molecular weight distribution of krill peptide prepared by the present invention, middle-molecular-weihydroxyethyl 12384Da, 6512Da,
The curve of 1423Da, 451Da and 189Da are respectively the chromatogram of corresponding molecular weight standards.
Fig. 3: the influence for activated carbon dosage to percent of decolourization and protein lost, wherein 1 refers to percent of decolourization, 2 fingers
It is protein lost.
Specific embodiment
Term as used in the present invention generally has those of ordinary skill in the art usual unless otherwise specified
The meaning of understanding.
Combined with specific embodiments below, and referring to the data further detailed description present invention.Following embodiment only be
It illustrates the present invention, rather than limits the scope of the invention in any way.
Molecular weight determination is referring to opposite point in GB/T31645-2018 " national food safety standard collagen peptide "
The detection method (Efficient numerical method method) of collagen peptide proportion of the son amount less than 10000.
Embodiment 1
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle alkali protease (the enzyme activity 2 × 10 that 2% Henan Yangshao Biochemical Engineering Co., Ltd. is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after digesting solution cooling, obtain solid residue and filtrate, the filtrate is
The krill albumen peptide solution digested from degreasing krill meal, color are dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 50min,
Fig. 3 is influence of the activated carbon dosage to percent of decolourization and protein lost；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain activity
Krill oligopeptides product.Oligopeptides yield is 90.1%, and degreasing krill meal protein extracting ratio is 95.5%, F-Content is 65 μ g/
g、Na+Content is 49mg/100g, K+Content is 155mg/Kg, Ca2+Content is 3500mg/Kg, Mg2+Content be 3500mg/Kg,
Average molecular weight is 364Da, and middle-molecular-weihydroxyethyl 1000Da protein hydrolysate proportion below is 88.8%, the phosphorus of preparation
The graph of molecular weight distribution of shrimp peptide is as shown in Fig. 2, its middle-molecular-weihydroxyethyl is 12384Da, 6512Da, 1423Da, 451Da and 189Da's
Curve is respectively the chromatogram of corresponding molecular weight standards.
Embodiment 2
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
The middle alkali protease (2 × 105u/g of enzyme activity) that 2% Pangbo Bioengineering Co Ltd, Nanning is added, 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 50min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 91.3%, and the protein extracting ratio of degreasing krill meal is 96.7%, F-Content is 75 μ
g/g、Na+Content is 54mg/100g, K+Content is 158mg/Kg, Ca2+Content is 3816mg/Kg, Mg2+Content is 4125mg/
Kg, average molecular weight 370Da, middle-molecular-weihydroxyethyl are 87.5% in 1000Da protein hydrolysate proportion below.
Embodiment 3
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle alkali protease (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 50min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 86.3%, and the protein extracting ratio of degreasing krill meal is 96.8%, F-Content is 56 μ
g/g、Na+Content is 78mg/100g, K+Content is 167mg/Kg, Ca2+Content is 3981mg/Kg, Mg2+Content is 4253mg/
Kg, average molecular weight 337Da, molecular weight 1000Da protein hydrolysate proportion below are 89.6%.
Embodiment 4
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle alkali protease (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 0.5% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decolorations
50min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 89.3%, degreasing krill meal protein extracting ratio 95.3%, F-Content be 43 μ g/g,
Na+Content is 74mg/100g, K+Content is 182mg/Kg, Ca2+Content is 4815mg/Kg, Mg2+Content is 4531mg/Kg, puts down
Average molecular weight is 344Da, and molecular weight 1000Da protein hydrolysate proportion below is 87.3%.
Embodiment 5
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle alkali protease (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 2h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 40min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 85.9%, degreasing krill meal protein extracting ratio 95.1%, F-Content be 32 μ g/g,
Na+Content is 58mg/100g, K+Content is 142mg/Kg, Ca2+Content is 4981mg/Kg, Mg2+Content is 3234mg/Kg, puts down
Average molecular weight is 362Da, and molecular weight 1000Da protein hydrolysate proportion below is 89.4%.
Embodiment 6
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle flavor protease (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 40min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 87.8%, degreasing krill meal protein extracting ratio 96.3%, F-Content be 29 μ g/g,
Na+Content is 56mg/100g, K+Content is 138mg/Kg, Ca2+Content is 5019mg/Kg, Mg2+Content is 3326mg/Kg, puts down
Average molecular weight is 363Da, and molecular weight 1000Da protein hydrolysate proportion below is 87.8%.
Embodiment 7
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle pepsin (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 40min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 86.7%, degreasing krill meal protein extracting ratio 95.3%, F-Content be 80 μ g/g,
Na+Content is 60mg/100g, K+Content is 130mg/Kg, Ca2+Content is 3482mg/Kg, Mg2+Content is 3621mg/Kg, puts down
Average molecular weight is 358Da, and molecular weight 1000Da protein hydrolysate proportion below is 80.2%.
Embodiment 8
(1) it pretreatment of raw material: by degreasing krill meal and tap water according to 1:15 ratio washing by soaking, washs 3 times altogether, to certainly
So after sedimentation, tipping removes cleaning solution；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of distilled water 1:10, to mixed liquor
Middle compound protease (the enzyme activity 2 × 10 that 2% Hebei Ge Beida Biotechnology Co., Ltd is added5U/g), 60 DEG C of hydrolysis 4.5h；
(3) enzyme deactivation: after enzymatic hydrolysis a period of time, temperature rises to 90 DEG C of progress enzyme deactivations, enzyme deactivation 15min；
(4) it filters: being filtered by vacuum after the solution cooling after enzyme deactivation, obtain solid residue and filtrate, the filter
Liquid is the krill albumen peptide solution digested from degreasing krill meal, and color is dark brown；
(5) it decolourizes: in the active carbon for the enzymolysis liquid addition 1% that above-mentioned suction filtration obtains, being uniformly mixed 30 DEG C of decoloration 40min；
(6) it filters: above-mentioned mixed liquor is filtered by vacuum, resulting filter cake is recycling, filter after active carbon recycling
Liquid is the krill albumen peptide solution after decoloration, yellowish；
(7) dry: above-mentioned solution being frozen in -20 DEG C of freezings, freeze drier is put it into and is freeze-dried to obtain the South Pole
Krill active oligopeptide product.Oligopeptides yield is 85.4%, degreasing krill meal protein extracting ratio 95.6%, F-Content be 77 μ g/g,
Na+Content is 64mg/100g, K+Content is 135mg/Kg, Ca2+Content is 3432mg/Kg, Mg2+Content is 3412mg/Kg, puts down
Average molecular weight is 346Da, and molecular weight 1000Da protein hydrolysate proportion below is 82.3%.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (10)
1. a kind of preparation method of krill active oligopeptide, which is characterized in that steps are as follows:
(1) pretreatment of raw material: degreasing krill meal and water are uniformly mixed according to 1:10-1:100 ratio, after washing, collect solid；
(2) it digests: pretreated degreasing krill meal (by dry weight) being mixed with the ratio of water 1:2-1:50, into mixed liquor
Protease is added to be digested；
(3) enzyme deactivation: after enzymatic hydrolysis, 80-100 DEG C, enzyme deactivation 5-30min；
(4) it filters: being filtered after the solution cooling after enzyme deactivation, separating solid residue and filtrate；
(5) it decolourizes: carrying out decolorization in the active carbon of above-mentioned filtrate addition 0.2%-5%；
(6) it filters: being filtered to remove active carbon, obtain destainer；
(7) dry: above-mentioned destainer is dried to obtain krill active oligopeptide.
2. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that degreasing phosphorus in step (1)
The amount ratio of shrimp med and water is 1:50, point 2~6 washings.
3. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that the albumen in step (2)
Enzyme is at least one of alkali protease, flavor protease, pepsin, compound protease.
4. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that enzymatic hydrolysis is in step (2)
10-70 DEG C, keep the temperature 2-12h.
5. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that filtering side in step (4)
Formula is vacuum filtration, vacuum degree: 0.08-- 0.01MPa；The suction filtration time is 5-15min.
6. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that the decoloration in step (5)
Processing is 30 DEG C, 40~50min.
7. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that filtering side in step (6)
Formula is vacuum filtration, vacuum degree: 0.08-- 0.01MPa；Filter the time: 10-20min.
8. the preparation method of krill active oligopeptide according to claim 1, which is characterized in that the drying in step (7)
To be spray-dried or being freeze-dried.
9. the krill active oligopeptide of any one of claim 1~8 the method preparation.
10. application of the krill active oligopeptide as claimed in claim 9 in medicine, functional food, cosmetics.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731512A (en) * | 2019-11-12 | 2020-01-31 | 大连工业大学 | Antarctic krill iron chelate peptide and preparation method and application of iron chelate peptide and iron chelate thereof |
| CN112342260A (en) * | 2020-11-11 | 2021-02-09 | 上海海洋大学 | Method for preparing blood sugar lowering peptide by using degreased euphausia superba powder and product thereof |
| CN112602892A (en) * | 2020-12-14 | 2021-04-06 | 中国水产科学研究院黄海水产研究所 | Yellow serofluid fermented beverage containing Antarctic krill peptide and preparation method thereof |
| CN110563808B (en) * | 2019-09-26 | 2021-04-20 | 浙江海洋大学 | Euphausia superba antioxidant oligopeptide and preparation method thereof |
| CN112956699A (en) * | 2021-01-27 | 2021-06-15 | 上海海洋大学 | Hypoglycemic peptide enteric solid dispersion and preparation method thereof |
| CN113429458A (en) * | 2021-08-03 | 2021-09-24 | 浙江海洋大学 | Euphausia superba blood sugar-reducing oligopeptide capable of inhibiting dipeptidyl peptidase IV function and application thereof |
| CN115843993A (en) * | 2022-11-08 | 2023-03-28 | 浙江工业大学 | Umami peptide and preparation method and application thereof |
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| CN108588157A (en) * | 2018-04-24 | 2018-09-28 | 大连理工大学 | The method that combination enzymic degradation degreasing euphausia superba powder recycles polypeptide intermediate product and prepares N-Acetyl-D-glucosamine |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110563808B (en) * | 2019-09-26 | 2021-04-20 | 浙江海洋大学 | Euphausia superba antioxidant oligopeptide and preparation method thereof |
| CN110731512A (en) * | 2019-11-12 | 2020-01-31 | 大连工业大学 | Antarctic krill iron chelate peptide and preparation method and application of iron chelate peptide and iron chelate thereof |
| CN112342260A (en) * | 2020-11-11 | 2021-02-09 | 上海海洋大学 | Method for preparing blood sugar lowering peptide by using degreased euphausia superba powder and product thereof |
| CN112602892A (en) * | 2020-12-14 | 2021-04-06 | 中国水产科学研究院黄海水产研究所 | Yellow serofluid fermented beverage containing Antarctic krill peptide and preparation method thereof |
| CN112956699A (en) * | 2021-01-27 | 2021-06-15 | 上海海洋大学 | Hypoglycemic peptide enteric solid dispersion and preparation method thereof |
| CN113429458A (en) * | 2021-08-03 | 2021-09-24 | 浙江海洋大学 | Euphausia superba blood sugar-reducing oligopeptide capable of inhibiting dipeptidyl peptidase IV function and application thereof |
| CN113429458B (en) * | 2021-08-03 | 2022-07-01 | 浙江海洋大学 | Euphausia superba blood glucose-reducing oligopeptide capable of inhibiting dipeptidyl peptidase IV function and application thereof |
| CN115843993A (en) * | 2022-11-08 | 2023-03-28 | 浙江工业大学 | Umami peptide and preparation method and application thereof |
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