CN109762739A - A kind of CIK cell culture apparatus with buffer protection - Google Patents
A kind of CIK cell culture apparatus with buffer protection Download PDFInfo
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- CN109762739A CN109762739A CN201811537959.3A CN201811537959A CN109762739A CN 109762739 A CN109762739 A CN 109762739A CN 201811537959 A CN201811537959 A CN 201811537959A CN 109762739 A CN109762739 A CN 109762739A
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- buffer
- box
- baffle
- fixedly connected
- box body
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Abstract
The invention patent discloses a kind of CIK cell culture apparatus with buffer protection, including box body, box cover, the lower section of box body is equipped with protection mechanism, protection mechanism includes that upper end opens up set casing for opening, the surrounding inner sidewall of set casing is fixedly connected with the same baffle-box by multiple damping springs, the bottom interior wall of set casing is uniformly provided with multiple ball grooves, Universal caster is fixedly connected with by installing mechanism in ball grooves, the inner wall of baffle-box slidably connects buffer board, the lower end of buffer board is connected by the bottom interior wall of multiple buffer gears and baffle-box, the upper end of buffer board is uniformly fixedly connected with multiple supporting blocks, the upper end of multiple supporting blocks passes through the opening that baffle-box upper end opens up, and to stretch out baffle-box outer and be fixedly connected with the same upper end and open up fixed frame for opening, box body is connected in fixed frame.The invention patent provides multi-buffer damping for box body, preferably avoids shaking force and be easy to cause the problem of damaging box body and influence its firm placement.
Description
Technical field
The invention patent relates to field of biotechnology more particularly to a kind of CIK cell culture apparatus with buffer protection.
Background technique
The research and development application of CIK cell is also increasingly goed deep into recent years, has formd more mature CIK cell
Induction and cultural method, but the reagent used in the incubation of CIK cell is more, and standard is inconsistent, in incubation
Heavy workload, incubation time is longer, and microbiological contamination risk is big, is difficult the staff of many researchers especially situation of all-level hospitals
The uniform CIK cell of culture mass in a short time.
Through retrieving, it is a kind of with buffer protection that license notification number is that the patent of CN 202107707 U proposes
CIK cell culture apparatus, including box body, box cover place reagent rack in the box body, and there are six insert in the reagent rack
Hole, the two rows of arrangements of the jack point；It is placed with interior dress lymphocyte separation medium, free serum culture respectively in six jacks
Base, inducible factor people recombinate 2 α of IL-1, inducible factor anti-CD49d McAb, inducible factor people recombinant il-2 and inducible factor IFN-γ
Reagent bottle；It is corresponded in the reagent rack and is respectively equipped with label on reagent bottle position and reagent bottle, which shortens culture
The time of CIK cell, but there is no preferable buffer protection structure for kit, it is easy to make in the impact by external force
The damage of pairs of kit.
Patent of invention content
The purpose of the invention patent be in order to solve there is no preferable buffer protection structure for kit in the prior art, by
To external force impact when be easy to cause the damage to kit the problem of, and propose a kind of CIK with buffer protection
Cell culture apparatus.
To achieve the goals above, the invention patent adopts the technical scheme that
A kind of CIK cell culture apparatus with buffer protection, including box body, box cover, place reagent rack in the box body,
There are six jack, the two rows of arrangements of the jack point in the reagent rack；It is placed with interior dress lymph respectively in six jacks
Cell separating liquid, serum free medium, inducible factor people recombinate 2 α of IL-1, inducible factor anti-CD49d McAb, inducible factor people weight
The reagent bottle of group IL-2 and inducible factor IFN-γ；It corresponds in the reagent rack and is respectively equipped on reagent bottle position and reagent bottle
Label, the lower section of the box body are equipped with protection mechanism, and the protection mechanism includes that upper end opens up set casing for opening, described solid
The surrounding inner sidewall for determining shell is fixedly connected with the same baffle-box by multiple damping springs, and the bottom interior wall of the set casing is equal
It is even to offer multiple ball grooves, the bottom for support baffle-box is fixedly connected with by installing mechanism and makes to buffer in ball grooves
Case is capable of the Universal caster of smooth sliding, and the inner wall of the baffle-box slidably connects buffer board, and the lower end of the buffer board is logical
The bottom interior wall for crossing multiple buffer gears and baffle-box connects, and the upper end of the buffer board is uniformly fixedly connected with multiple supports
Block, the upper end of multiple supporting blocks pass through the opening that baffle-box upper end opens up and stretch out outside baffle-box and be fixedly connected with same
The fixed frame that a upper end opens up as opening is connected with box body, the upper end of the buffer board and the top of baffle-box in the fixed frame
Multiple buffer springs are uniformly fixedly connected between portion's inner wall, it is uniformly solid between the upper end of the baffle-box and the lower end of fixed frame
Surely multiple rubber buffer rings are connected with.
Preferably, the buffer gear includes two buffer bars hinged with buffer board lower end and in splayed distribution, and two
The same reset spring is fixedly connected between buffer bar described in root, the lower end of the buffer bar is rotatably connected to buffer sliding block,
The bottom interior wall of the baffle-box offers the buffering chute to match with buffer sliding block.
Preferably, the inner wall that the fixed frame corresponds to box body four corners offers cylinder groove, is equipped in the cylinder groove
Thrust spring, one end of the thrust spring are fixedly connected with the slot bottom of cylinder groove, and the other end of the thrust spring is fixed to be connected
It is connected to gag lever post, the outer wall of the box body offers limiting slot corresponding with gag lever post, and the gag lever post is located in cylinder groove
One end is fixedly connected with pull rod, and the one end of the pull rod far from gag lever post stretches out fixed outer frame by through-hole and is fixedly connected with drawing
Plate.
Preferably, the side wall of the buffer board is uniformly fixedly connected with multiple position limiting slide blocks, the baffle-box it is vertical in
Side wall offers the limit sliding chutes to match with position limiting slide block.
Preferably, the upper bar wall of the gag lever post is set as inclined-plane.
Compared with prior art, the invention patent provides a kind of CIK cell culture apparatus with buffer protection, has
Below the utility model has the advantages that
1, the CIK cell culture apparatus with buffer protection, when box body is by extraneous vibratory forces, impact force, which drives, to be fixed
Shell vibration, set casing squeeze baffle-box by damping spring, can be preferable right using the screen resilience that damping spring deformation generates
Lateral shaking force carries out bumper and absorbing shock, and vibratory forces shake box body, and box body drives fixed frame to squeeze rubber buffer ring first, benefit
First-level buffer damping is provided for box body with the screen resilience that rubber buffer ring deformation generates, fixed frame is squeezed by supporting block buffer again
Plate, buffer board pull buffer spring, provide second level bumper and absorbing shock to box body using the screen resilience that buffer spring deformation generates, delay
Punching squeezes buffer gear again, the movement that two buffer bars do mutually to deviate from using sliding of the buffer sliding block in buffering chute,
Reset spring is pulled back two buffer bars, provides third level bumper and absorbing shock to box body using the screen resilience that reset spring deformation generates
Power can carry out preferable multi-buffer damping to the shaking force of box body vertical direction, provide multi-buffer damping for box body, compared with
The good shaking force that avoids be easy to cause the problem of damaging box body and influence its firm placement.
2, CIK cell culture apparatus with buffer protection, by the fixed frame being equipped with, when installing box body, by box
Body lower end is pressed into fixed frame, and the lower end of box body squeezes the inclined-plane of gag lever post, and gag lever post stress is made to squeeze automatic retraction cylinder
In slot, when the limiting slot on box body is moved at gag lever post, thrust spring pushes gag lever post, and gag lever post is released outside cylinder groove
And engage gag lever post with limiting slot and being bonded between box body and fixed frame can be realized, it is needing to clean box body
When disinfection, pulling plate is pulled, gag lever post is pulled out to the relative limit locking that can be released outside limiting slot to box body using pull rod, it can
It realizes the fast assembling-disassembling to box body, is easy to use.
And be not directed to part in the device and be the same as those in the prior art or can be realized by using the prior art, the present invention
Patent provides multi-buffer damping for box body, preferably avoids shaking force and be easy to cause and damages box body and influence it and firm put
The problem of setting.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram for CIK cell culture apparatus with buffer protection that the invention patent proposes；
Fig. 2 is a kind of structural schematic diagram for CIK cell culture apparatus part A with buffer protection that the invention patent proposes；
Fig. 3 is a kind of structural schematic diagram for CIK cell culture apparatus part B with buffer protection that the invention patent proposes.
In figure: 1 box body, 2 box covers, 3 set casings, 4 damping springs, 5 baffle-boxs, 6 Universal casters, 7 buffer boards, 8 supporting blocks,
9 fixed frames, 10 buffer springs, 11 rubber buffer rings, 12 buffer bars, 13 reset springs, 14 buffer sliding blocks, 15 buffering chutes, 16
Cylinder groove, 17 thrust springs, 18 gag lever posts, 19 limiting slots, 20 pull rods, 21 pulling plates.
Specific embodiment
Below in conjunction with the attached drawing in the invention patent embodiment, the technical solution in the invention patent embodiment is carried out
Clearly and completely describing, it is clear that described embodiment is only the invention patent a part of the embodiment, rather than whole
Embodiment.
In the description of the invention patent, it is to be understood that term " on ", "lower", "front", "rear", "left", "right",
The orientation or positional relationship of the instructions such as "top", "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the invention patent and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as the limitation to the invention patent.
Referring to Fig.1-3, a kind of CIK cell culture apparatus with buffer protection, including box body 1, box cover 2, box body 1
Interior placement reagent rack, reagent rack on there are six jack, the two rows of arrangements of jack point；It is placed with interior dress respectively in six jacks
Lymphocyte separation medium, serum free medium, inducible factor people recombinate 2 α of IL-1, inducible factor anti-CD49d McAb, inducible factor
The reagent bottle of people's recombinant il-2 and inducible factor IFN-γ；It corresponds in reagent rack and is respectively equipped on reagent bottle position and reagent bottle
Label, the lower section of box body 1 are equipped with protection mechanism, and protection mechanism includes that upper end opens up set casing 3 for opening, and the four of set casing 3
All inner sidewalls are fixedly connected with the same baffle-box 5 by multiple damping springs 4, and the bottom interior wall of set casing 3 is uniformly provided with
Multiple ball grooves, ball grooves are interior to be fixedly connected with the bottom for support baffle-box 5 by installing mechanism and enables baffle-box 5
The Universal caster 6 of smooth sliding does not repeat excessively herein wherein the installing mechanism for Universal caster is the prior art, buffers
The inner wall of case 5 slidably connects buffer board 7, and the lower end of buffer board 7 is connected by the bottom interior wall of multiple buffer gears and baffle-box 5
It connects, the upper end of buffer board 7 is uniformly fixedly connected with multiple supporting blocks 8, and the upper end of multiple supporting blocks 8 passes through 5 upper end of baffle-box
It is outer and be fixedly connected with the same upper end and open up fixed frame 9 for opening, card in fixed frame 9 that the opening opened up stretches out baffle-box 5
It is connected to box body 1, multiple buffer springs 10 are uniformly fixedly connected between the upper end of buffer board 7 and the top inner wall of baffle-box 5, is delayed
It rushes between the upper end of case 5 and the lower end of fixed frame 9 and is uniformly fixedly connected with multiple rubber buffer rings 11.
Buffer gear includes two buffer bars 12 hinged with 7 lower end of buffer board and in splayed distribution, two buffer bars
The same reset spring 13 is fixedly connected between 12, the lower end of buffer bar 12 is rotatably connected to buffer sliding block 14, baffle-box 5
Bottom interior wall offers the buffering chute 15 to match with buffer sliding block 14.
The inner wall of corresponding 1 four corners of box body of fixed frame 9 offers cylinder groove 16, is equipped with thrust spring in cylinder groove 16
17, one end of thrust spring 17 is fixedly connected with the slot bottom of cylinder groove 16, and the other end of thrust spring 17 is fixedly connected with limit
Bar 18, the outer wall of box body 1 offer limiting slot 19 corresponding with gag lever post 18, and it is solid that gag lever post 18 is located at one end in cylinder groove 16
Surely it is connected with pull rod 20, the one end of pull rod 20 far from gag lever post 18 is stretched out outside fixed frame 9 by through-hole and is fixedly connected with pulling plate
21。
The side wall of buffer board 7 is uniformly fixedly connected with multiple position limiting slide blocks, and the vertical inner sidewall of baffle-box 5 is offered and limited
The limit sliding chutes that position sliding block matches.
The upper bar wall of gag lever post 18 is set as inclined-plane.
In the invention patent, in use, impact force drives set casing 3 to shake when box body 1 is by extraneous vibratory forces,
Set casing 3 squeezes baffle-box 5 by damping spring 4, can be preferably to transverse direction using the screen resilience that 4 deformation of damping spring generates
Shaking force carry out bumper and absorbing shock, vibratory forces make box body 1 shake, box body 1 drive fixed frame 9 squeeze rubber buffer ring 11 first,
The screen resilience generated using 11 deformation of rubber buffer ring is that box body 1 provides first-level buffer damping, and fixed frame 9 passes through supporting block 8 again
Buffer board 7 is squeezed, buffer board 7 pulls buffer spring 10, and the screen resilience generated using 10 deformation of buffer spring provides the to box body 1
Level 2 buffering damping, buffer board 7 squeeze buffer gear again, and two buffer bars 12 are using buffer sliding block 14 in buffering chute 15
The movement mutually deviated from is done in sliding, and reset spring 13 is pulled back two buffer bars 12, the rebound for utilizing 13 deformation of reset spring to generate
Power provides third level bumper and absorbing shock power to box body 1, can carry out preferable multi-buffer to the shaking force of 1 vertical direction of box body and subtract
Shake provides multi-buffer damping for box body 1, preferably avoids shaking force and be easy to cause that damage box body 1 and influence it firm
The problem of placement, when installing box body 1,1 lower end of box body is pressed into fixed frame 9 by the fixed frame 9 being equipped with, box body 1
Lower end squeezes the inclined-plane of gag lever post 18, squeezes 18 stress of gag lever post in automatic retraction cylinder groove 16, the limiting slot on box body 1
19 when being moved at gag lever post 18, and thrust spring 17 pushes gag lever post 18, and it is outer and make to limit that gag lever post 18 is released cylinder groove 16
Bar 18 engages with limiting slot 19 can be realized being bonded between box body 1 and fixed frame 9, need to clean box body 1
When disinfection, pulling plate 21 is pulled, gag lever post 18 is pulled out to the relative limit that can be released outside limiting slot 19 to box body 1 using pull rod 20
Locking, can be realized the fast assembling-disassembling to box body 1, be easy to use.
The preferable specific embodiment of the above, only the invention patent, but the scope of protection of the patent of the present invention is not
It is confined to this, anyone skilled in the art is in the technical scope that the invention patent discloses, according to the present invention
Technical solution and its patent of invention design of patent are subject to equivalent substitution or change, should all cover the protection model in the invention patent
Within enclosing.
Claims (5)
1. a kind of CIK cell culture apparatus with buffer protection, including box body (1), box cover (2), in the box body (1)
Reagent rack is placed, there are six jack, the two rows of arrangements of the jack point in the reagent rack；It is placed respectively in six jacks
There are built-in lymphocyte separation medium, serum free medium, inducible factor people to recombinate 2 α of IL-1, inducible factor anti-CD49d McAb, lure
The reagent bottle of inducement Ziren recombinant il-2 and inducible factor IFN-γ；Reagent bottle position and reagent bottle are corresponded in the reagent rack
On be respectively equipped with label, which is characterized in that the lower section of the box body (1) be equipped with protection mechanism, the protection mechanism includes upper end
The set casing (3) for opening is opened up, the surrounding inner sidewall of the set casing (3) is fixedly connected with by multiple damping springs (4)
The bottom interior wall of the same baffle-box (5), the set casing (3) is uniformly provided with multiple ball grooves, passes through installation in ball grooves
Mechanism is fixedly connected with the bottom for support baffle-box (5) and baffle-box (5) is made to be capable of the Universal caster (6) of smooth sliding,
The inner wall of the baffle-box (5) slidably connects buffer board (7), the lower end of the buffer board (7) by multiple buffer gears with
The bottom interior wall of baffle-box (5) connects, and the upper end of the buffer board (7) is uniformly fixedly connected with multiple supporting blocks (8), Duo Gesuo
The upper end for stating supporting block (8) passes through the opening that baffle-box (5) upper end opens up and stretches out baffle-box (5) outside and be fixedly connected with same
The fixed frame (9) that a upper end opens up as opening, the fixed frame (9) is interior to be connected with box body (1), the upper end of the buffer board (7)
Uniformly be fixedly connected between the top inner wall of baffle-box (5) multiple buffer springs (10), the upper end of the baffle-box (5) with
Multiple rubber buffer rings (11) are uniformly fixedly connected between the lower end of fixed frame (9).
2. a kind of CIK cell culture apparatus with buffer protection according to claim 1, which is characterized in that described slow
Punch mechanism includes two hinged with buffer board (7) lower end and in the buffer bar (12) that splayed is distributed, two buffer bars
(12) it is fixedly connected between the same reset spring (13), the lower end of the buffer bar (12) is rotatably connected to buffer sliding block
(14), the bottom interior wall of the baffle-box (5) offers the buffering chute (15) to match with buffer sliding block (14).
3. a kind of CIK cell culture apparatus with buffer protection according to claim 1, which is characterized in that described solid
The inner wall for determining frame (9) corresponding box body (1) four corners offers cylinder groove (16), and thrust spring is equipped in the cylinder groove (16)
(17), one end of the thrust spring (17) is fixedly connected with the slot bottom of cylinder groove (16), the thrust spring (17) it is another
End is fixedly connected with gag lever post (18), and the outer wall of the box body (1) offers limiting slot (19) corresponding with gag lever post (18), institute
It states one end that gag lever post (18) is located in cylinder groove (16) to be fixedly connected with pull rod (20), the pull rod (20) is far from gag lever post
(18) one end stretches out fixed frame (9) outside by through-hole and is fixedly connected with pulling plate (21).
4. a kind of CIK cell culture apparatus with buffer protection according to claim 1, which is characterized in that described slow
The side wall of punching (7) is uniformly fixedly connected with multiple position limiting slide blocks, and the vertical inner sidewall of the baffle-box (5) is offered and limited
The limit sliding chutes that sliding block matches.
5. a kind of CIK cell culture apparatus with buffer protection according to claim 3, which is characterized in that the limit
The upper bar wall of position bar (18) is set as inclined-plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811537959.3A CN109762739A (en) | 2018-12-16 | 2018-12-16 | A kind of CIK cell culture apparatus with buffer protection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811537959.3A CN109762739A (en) | 2018-12-16 | 2018-12-16 | A kind of CIK cell culture apparatus with buffer protection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109762739A true CN109762739A (en) | 2019-05-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811537959.3A Pending CN109762739A (en) | 2018-12-16 | 2018-12-16 | A kind of CIK cell culture apparatus with buffer protection |
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| Country | Link |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110143123A (en) * | 2019-06-06 | 2019-08-20 | 南京理工自动化研究院有限公司 | A kind of electric automobile power battery frame lifting device and elevating method |
| CN111779677A (en) * | 2020-07-21 | 2020-10-16 | 文家洪 | Air compressor silencer |
| CN112212988A (en) * | 2020-11-03 | 2021-01-12 | 安徽安广电气有限公司 | Temperature instrument with good shock resistance |
| CN112939144A (en) * | 2021-02-05 | 2021-06-11 | 绍兴市慧融臻合新能源科技有限公司 | Domestic sewage treatment equipment |
| CN113040131A (en) * | 2021-03-17 | 2021-06-29 | 河南和泽干细胞基因工程有限公司 | Cell preservation device capable of preventing cell pollution |
| CN114749389A (en) * | 2021-12-15 | 2022-07-15 | 深圳市鸿昇智能科技有限公司 | Automatic sorting integrated system |
-
2018
- 2018-12-16 CN CN201811537959.3A patent/CN109762739A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110143123A (en) * | 2019-06-06 | 2019-08-20 | 南京理工自动化研究院有限公司 | A kind of electric automobile power battery frame lifting device and elevating method |
| CN111779677A (en) * | 2020-07-21 | 2020-10-16 | 文家洪 | Air compressor silencer |
| CN112212988A (en) * | 2020-11-03 | 2021-01-12 | 安徽安广电气有限公司 | Temperature instrument with good shock resistance |
| CN112939144A (en) * | 2021-02-05 | 2021-06-11 | 绍兴市慧融臻合新能源科技有限公司 | Domestic sewage treatment equipment |
| CN113040131A (en) * | 2021-03-17 | 2021-06-29 | 河南和泽干细胞基因工程有限公司 | Cell preservation device capable of preventing cell pollution |
| CN114749389A (en) * | 2021-12-15 | 2022-07-15 | 深圳市鸿昇智能科技有限公司 | Automatic sorting integrated system |
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Application publication date: 20190517 |