CN109759441B - Saline-alkali soil remediation method - Google Patents
Saline-alkali soil remediation method Download PDFInfo
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- CN109759441B CN109759441B CN201910176612.9A CN201910176612A CN109759441B CN 109759441 B CN109759441 B CN 109759441B CN 201910176612 A CN201910176612 A CN 201910176612A CN 109759441 B CN109759441 B CN 109759441B
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Abstract
The invention provides a saline-alkali soil remediation method, and belongs to the field of contaminated soil remediation. The method comprises the following steps: 1) in winter, applying the modifying agent into saline-alkali soil, spraying bamboo vinegar, and ploughing; the mass of the bamboo vinegar liquid is 0.5-1% of that of the modifying agent; the effective components of the modifying agent comprise phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure; 2) in spring, applying trichoderma virens to the ploughed soil; and planting atriplex canescens and sesbania in intercropping mode. The method of the invention utilizes the complementary advantages of the symbiotic system formed by the atriplex canescens-sesbania intercropping, the improvement effect of the modifier and the growth promotion effect of trichoderma viride, promotes the joint production increase of the atriplex canescens and the sesbania, and improves the repair efficiency of plants to the saline-alkali soil.
Description
Technical Field
The invention belongs to the field of polluted soil remediation, and particularly relates to a saline-alkali soil remediation method.
Background
The problems of salinization and secondary salinization of soil become one of the main ecological environmental problems to be solved urgently in arid and semiarid regions in China. Soil salinization can cause land degradation, weaken and destroy land productivity, reduce crop yield, and is a main obstacle for restricting agricultural development in arid regions, and a scientific and reasonable method is needed for solving the problem. In addition, the continuously enhanced human activities enable heavy metals such as cadmium, lead, zinc, chromium and the like to be continuously released into the environment, so that the soil environment and the ecological system are greatly impacted and damaged, and the heavy metal pollution increasingly becomes a potential risk of the ecological system of the saline-alkali soil. Therefore, saline-alkali soil remediation and toxic heavy metal prevention and control in a soil system are always difficult points and hot points for soil ecology research.
Compared with the common saline-alkali soil, the saline-alkali soil polluted by heavy metal is more difficult to improve and treat, and firstly, the pollutants have poor mobility and long retention time in the soil and cannot be naturally degraded, diluted and flowed, and are increasingly enriched in the saline-alkali soil along with the prolonging of time, so that plants planted in the saline-alkali soil are harmed; secondly, because the salt content is high and alkaline, the survival rate of the plant is very low, and therefore, the natural improvement and the exertion effect are very slow.
At present, an effective method for repairing saline-alkali soil is not provided, and the saline-alkali soil can be effectively repaired.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for repairing saline-alkali soil, which can effectively improve the soil environment of crops, increase the biomass of crops, and improve the repairing efficiency of saline-alkali soil.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a saline-alkali soil remediation method, which comprises the following steps:
1) in winter, applying the modifying agent into saline-alkali soil, spraying bamboo vinegar, and ploughing; the mass of the bamboo vinegar liquid is 0.5-1% of that of the modifying agent;
the effective components of the modifying agent comprise phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure;
2) in the spring of the next year, applying trichoderma virens into the ploughed soil; and planting atriplex canescens and sesbania in intercropping mode.
Preferably, the saline-alkali soil comprises a heavy metal polluted saline-alkali soil.
Preferably, the effective components of the modifying agent in the step 1) comprise the following components in parts by weight: 4-6 parts of phosphogypsum, 1-1.5 parts of calcium superphosphate and 5-10 parts of decomposed livestock and poultry manure.
Preferably, the CaSO in the phosphogypsum4·2H2The O content is not lower than 90%, and the application amount is 200-300 kg/mu; the calcium superphosphate contains not less than 11% of Ca, not less than 14% of phosphorus and the application amount is 50-75 kg/mu; the organic matter content of the decomposed livestock and poultry manure is not lower than 30g/kg, and the application amount is 250-500 kg/mu.
Preferably, the preparation method of the decomposed livestock and poultry manure comprises the following steps: mixing livestock and poultry manure, urea, calcium superphosphate and premix to obtain a mixture, adjusting the water content of the mixture to be 45% -55%, and fermenting for 21-28 days; the premix comprises 0.5-1.5 parts of quick-rotting agent, 15-20 parts of bran and 20-30 parts of corn flour.
Preferably, in the fermentation process, the pile is turned once every 7-10 days.
Preferably, the trichoderma virens microbial inoculum in the step 2) comprises a trichoderma virens F7 strain, and the preservation number of the trichoderma virens F7 strain is CGMCCNo. 3.17613; the spore amount in the microbial inoculum is 6 multiplied by 108～5×109Per gram.
Preferably, the application amount of the microbial inoculum in the step 2) is 30-50 kg/mu.
Preferably, when the atriplex canescens and the sesbania are planted in the intercropping mode in the step 3), 1-3 rows of the sesbania are arranged between every two rows of the atriplex canescens, the distance between every two adjacent rows of the atriplex canescens and the sesbania is 45-60 cm, the distance between every two adjacent rows of the sesbania is 45-60 cm, the plant spacing of the atriplex canescens is 25-30 cm, and the plant spacing of the sesbania is 30-35 cm.
Preferably, before planting in the step 3), the method further comprises the step of disinfecting the seeds of the atriplex canescens and the sesbania sessilifolia, wherein the disinfection comprises the steps of disinfecting for 25-35 min by using 4-6% of sodium hypochlorite, washing by using tap water, and soaking for 6-10 h in 6-8 mmol/L gamma-aminobutyric acid.
The invention provides a saline-alkali soil remediation method, which utilizes the complementary advantages of a symbiotic system formed by atriplex canescens-sesbania intercropping, the improvement effect of a modifier and the growth promotion effect of trichoderma viride, promotes the joint production increase of atriplex canescens and sesbania, and improves the remediation efficiency of plants on the saline-alkali soil.
In the embodiment of the invention, the cadmium content of the overground part and the underground part of the atriplex canescens under the atriplex canescens intercropping mode is respectively improved by 6.90-11.8 percent and 7.23-12.5 percent compared with the cadmium content of the single atriplex canescens, and the cadmium content of the overground part and the underground part of the atriplex canescens under the atriplex canescens intercropping mode is respectively improved by 6.90-11.8 percent and 7.23-12.5 percent compared with the cadmium content of the single canescens, so that the crop intercropping advantage is exerted, the cadmium content of intercropping crops is obviously improved, and the effect of obviously reducing the heavy.
Under the mode of the atriplex canescens intercropping, the biomass of the aboveground part and the underground part of the atriplex canescens are respectively increased by 12.5 to 13.5 percent and 12.3 to 18.2 percent compared with the atriplex canescens intercropping, and the biomass of the aboveground part and the underground part of the atriplex canescens during the atriplex canescens intercropping are respectively increased by 11.2 to 13.3 percent and 18.0 to 22.9 percent compared with the monocnantha canescens intercropping, which shows that the intercropping advantage of crops is exerted, and the biomass of the.
Biological preservation Instructions
Trichoderma viride (Trichoderma virens), deposited at the institute for microbiology, academy of sciences, China, at the deposit location: the biological preservation number is CGMCC No.3.17613, the strain number is as follows: f7 JX 993849.
Drawings
FIG. 1 is a plot of the relationship between atriplex canescens and sesbania as in example 1;
FIG. 2 is a plot of the relationship between atriplex canescens and sesbania as in example 2;
Detailed Description
The invention provides a saline-alkali soil remediation method, which comprises the following steps:
1) in winter, applying the modifying agent into saline-alkali soil, spraying bamboo vinegar, and ploughing; the mass of the bamboo vinegar liquid is 0.5-1% of that of the modifying agent;
the effective components of the modifying agent comprise phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure;
2) in the spring of the next year, applying trichoderma virens into the ploughed soil; and planting atriplex canescens and sesbania in intercropping mode.
In the restoration method, in winter, the conditioner is applied to the saline-alkali soil, the bamboo vinegar is sprayed, and the soil is ploughed; the mass of the bamboo vinegar liquid is 0.5-1% of that of the modifying agent.
In the invention, the effective components of the modifier comprise phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure. In the modifying agent, the weight part of the phosphogypsum is preferably 4.5-5.5 parts, and more preferably 5 parts. CaSO in phosphogypsum4·2H2The O content is preferably not less than 91%, more preferably not less than 92%, most preferably not less than 93%. The application amount of the phosphogypsum is preferably 210-290 kg/mu, more preferably 220-280 kg/mu and most preferably 250 kg/mu. The phosphogypsum is acidic, has a pH value of 3-4, contains a large amount of nutrient elements required by plants such as sulfur, calcium and the like, and can increase the yield of crops, improve the quality and improve the soil. The source of the phosphogypsum of the invention is not particularly limited, and is preferably purchased from a Linyidenfeng gypsum product factory.
In the modifying agent, the weight part of the calcium superphosphate is preferably 1.2-1.4 parts, and more preferably 1.3 parts; the calcium content of said superphosphate is preferably not less than 12%, more preferably not less than 13%, most preferably not less than 14%; the phosphorus content of the superphosphate is preferably not less than 15%, more preferably not less than 16%, and most preferably not less than 17%. The application amount of the calcium superphosphate is preferably 52-73 kg/mu, more preferably 55-70 kg/mu and most preferably 57 kg/mu. In the invention, the calcium superphosphate can reduce the pH value of soil, and the phosphorus in the calcium superphosphate can improve the resistance of crops. The source of the calcium superphosphate of the present invention is not particularly limited, and is preferably purchased from Jinnskiang chemical Co.
The organic matter content of the decomposed livestock manure is preferably not less than 31g/kg, more preferably not less than 32g/kg, and most preferably not less than 33 g/kg. The application amount of the decomposed livestock and poultry manure is preferably 270-480 kg/mu, more preferably 300-450 kg/mu, and most preferably 400 kg/mu. The decomposed livestock and poultry manure is preferably decomposed chicken manure.
The preparation method of the decomposed livestock and poultry manure preferably comprises the steps of mixing the livestock and poultry manure, urea, calcium superphosphate and premix to obtain a mixture, adjusting the water content of the mixture to be 45% -55%, uniformly stirring, and fermenting for 21-28 days; the premix comprises 0.5-1.5 parts of quick-rotting agent, 15-20 parts of bran and 25-30 parts of corn flour. According to the mixing method, the urea, the calcium superphosphate and the premix are preferably sprayed into the livestock and poultry manure. Before the urea, the calcium superphosphate and the premix are sprayed, the method preferably also comprises the step of stacking the livestock and poultry manure into long strips to obtain the livestock and poultry manure compost. The addition amount of the urea is preferably 0.08-0.12% of the livestock and poultry manure compost, and more preferably 0.1% of the livestock and poultry manure compost in parts by weight. The addition amount of the calcium superphosphate is preferably 13-18 kg/ton, more preferably 14-16 kg/ton, and most preferably 15 kg/ton in parts by weight. The premix preferably comprises a quick-rotting agent, wherein the quick-rotting agent is preferably purchased from Henan Wobo biotechnology limited, and the addition amount of the quick-rotting agent is preferably 0.6-1.3 parts by weight, more preferably 0.7-1.2 parts by weight, and most preferably 1 part by weight. The premix preferably comprises bran, and the addition amount of the bran is preferably 16-19 parts by weight, more preferably 17-18 parts by weight, and most preferably 17.5 parts by weight. The premix preferably comprises corn flour, and the adding amount of the corn flour is preferably 26-29 parts by weight, more preferably 27-28 parts by weight, and most preferably 27.5 parts by weight. The preparation method of the premix comprises the step of uniformly mixing 0.5-1.5 parts of the quick-rotting agent, 15-20 parts of the bran and 25-30 parts of the corn flour.
The method preferably adds the crushed corn straws when the mixture is adjusted to be wet, wherein the preferable granularity of the crushed corn straws is 0.5-5 cm, more preferably 1-4 cm, and most preferably 2 cm. The moisture content is preferably 45% to 55%, more preferably 47% to 53%, and most preferably 50%. The fermentation time is preferably 21-28 d, more preferably 24-28 d, and most preferably 24 d. In the invention, the decomposed livestock and poultry manure can promote the growth of plants, improve the disease prevention and resistance of the plants and improve the utilization rate of fertilizer. The function of promoting the growth of the plants is realized by the planting life of functional bacteria for fermenting the livestock and poultry manure at the roots of the plants; the improvement of the disease prevention and resistance of the plants is achieved by the biological prevention and control effect of antagonistic plant pathogenic bacteria. The improvement of the utilization rate of the fertilizer is realized by the actions of nitrogen fixation, potassium dissolution, active phosphorus and the like.
The invention sprays bamboo vinegar after applying the modifier. The quality of the bamboo vinegar liquid is preferably 0.6-0.9%, more preferably 0.7-0.8%, and most preferably 0.75% of the quality of the modifier. In the invention, the bamboo vinegar is a liquid substance obtained by collecting gas generated by high-temperature decomposition of bamboo charcoal in the process of burning the bamboo charcoal and cooling the gas at normal temperature; the bamboo vinegar liquid is applied to soil, can effectively inhibit the propagation of microorganisms which hinder the growth of plants, and can kill pests such as root nodule nematodes and the like. The source of the bamboo vinegar solution of the present invention is not particularly limited, and it is preferably purchased from Shanxi Dewei Biochemical Co., Ltd.
The invention ploughs the soil after spraying the bamboo vinegar. The mode of the plowing is not particularly limited, and the plowing is preferably mechanical plowing, and the depth of the mechanical plowing is preferably 35-45 cm, more preferably 38-42 cm, and most preferably 40 cm. In the invention, the plowing can lead the modifying agent to be evenly mixed with the soil.
Before the sowing in spring, the trichoderma virens is applied to the ploughed soil. Before the trichoderma virens is applied, water is preferably added to submerge the ground surface, and after soaking, the water is drained. The soaking time is preferably 5-8 h, more preferably 6-7 h, and most preferably 6.5 h. The Trichoderma viride preparation comprises a Trichoderma viride F7 strain, wherein the Trichoderma viride F7 strain is classified and named as Trichoderma virens, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No.3 of Xilu No. 1 of Beijing Kogyo, Chaoyang, the preservation date is 12 months and 31 days in 2014, and the preservation number is CGMCC No. 3.17613. The trichoderma virens microbial inoculum is preferably a solid microbial inoculum. The spore amount in the microbial inoculum is preferably 7 multiplied by 108～4×109Per g, more preferably 8X 108～3×109Per g, most preferably 1X 109Per gram. The application amount of the trichoderma virens microbial inoculum is preferably 30-50 kg/mu, more preferably 35-45 kg/mu and most preferably 40 kg/mu.
The preparation method of the trichoderma virens microbial inoculum comprises the following steps:
a. activating the Trichoderma viride F7 strain, inoculating the Trichoderma viride F7 strain to a PDA slant culture medium, culturing at 28-32 ℃ for 5-7 days to obtain spores, diluting the spore liquid to 0.5-1.5 multiplied by 106Obtaining spore suspension;
b. under an aseptic condition, inoculating the spore suspension obtained in the step a into a PDA liquid culture medium, and culturing at the temperature of 28-32 ℃ for 70-74 h under the condition of 170-190 rpm to obtain a seed solution;
c. and inoculating the seed solution into a solid fermentation culture medium, and fermenting for 5-7 days to obtain the trichoderma virens microbial inoculum.
In the invention, the Trichoderma viride F7 strain is activated, inoculated on a PDA slant culture medium, cultured for 5-7 days at 28-32 ℃ to obtain spores, and the spore solution is diluted to (0.5-1.5) x 106And (4) obtaining spore suspension. The culture time in the present invention is preferably5-7 d, and more preferably 6 d. The culture temperature is preferably 28-32 ℃, more preferably 29-31 ℃, and most preferably 30 ℃. The dilution concentration of the spore liquid is preferably (0.7-1.2) x 106More preferably (0.8 to 1.1). times.10 per gram6Per g, most preferably 1.0X 106Per gram.
After obtaining the spore suspension, inoculating the spore suspension into a PDA liquid culture medium under an aseptic condition, and culturing at the temperature of 28-32 ℃ and the rpm of 170-190 for 70-74 hours to obtain a seed solution. The culture temperature is preferably 28-32 ℃, more preferably 29-31 ℃, and most preferably 30 ℃. The culture time is preferably 70-74 h, more preferably 71-73 h, and most preferably 72 h. The culture is preferably carried out under the shaking condition during the culture, and the culture rotating speed is preferably 170-190 rpm, more preferably 175-185 rpm, and most preferably 180 rpm. The PDA slant culture medium comprises the following components: 200g of potato, 20g of glucose, 20g of agar and 1000mL of water.
In the invention, the obtained seed solution is inoculated in a solid fermentation culture medium for fermentation for 5-7 days to obtain the trichoderma virens microbial inoculum. The inoculation amount of the seed liquid is preferably 8-12%, more preferably 9-11%, and most preferably 10%. The fermentation of the present invention is preferably aerobic fermentation, and the time for the aerobic fermentation is preferably 5 to 7 days, and more preferably 6 days. The fermentation temperature is preferably 28-32 ℃, more preferably 29-31 ℃, and most preferably 30 ℃. The spore amount in the microbial inoculum is preferably 7 multiplied by 108～4×109Per g, more preferably 8X 108～3×109Per g, most preferably 1X 109Per gram. The trichoderma virens F7 can effectively reduce the pH value of saline-alkali soil and improve the effective state content of heavy metal in the soil, thereby playing a role in activating the heavy metal. The solid fermentation medium comprises the following components: 30g of wheat bran, 20g of rice bran, 10g of corn flour, 5g of soybean meal, 5g of glucose, 3.0g of monopotassium phosphate, 1.5g of calcium sulfate, 1.5g of magnesium sulfate and 1000mL of water.
After trichoderma viride is applied, atriplex canescens and sesbania are intercropped and sowed. According to the invention, before the atriplex canescens and sesbania are sown in intercropping mode, sodium hypochlorite is preferably used for disinfecting the atriplex canescens and sesbania seeds. The concentration of the sodium hypochlorite is preferably 4-6%, and more preferably 5%. The disinfection time is preferably 25-35 min, more preferably 28-32 min, and most preferably 30 min. After the atriplex canescens and sesbania seeds are disinfected, the atriplex canescens and sesbania seeds are preferably soaked by gamma-aminobutyric acid. The concentration of the gamma-aminobutyric acid is preferably 7-8 mmol/L, and more preferably 7.5 mmol/L. The soaking time is preferably 6-10 h, more preferably 7-9 h, and most preferably 8 h. Before the present invention is used for sowing atriplex canescens and sesbania, the soil is preferably ditched. The trenching apparatus is preferably a seeding machine. After ditching, the seeds are sown along the furrows, the sowing mode is manual dibbling or hole sowing, the sowing depth is preferably 3-4 cm, more preferably 3.5cm, the sowing amount is preferably 3-7 seeds per hole, more preferably 4-6 seeds, and most preferably 5 seeds. When the atriplex canescens and sesbania are sowed, the row number ratio is preferably 1: (1-3), more preferably 1: 2; the row spacing between two adjacent rows of atriplex canescens and sesbania is preferably 47-58 cm, more preferably 50-55 cm, and most preferably 52 cm. The distance between two adjacent rows of sesbania is preferably 47-58 cm, more preferably 50-55 cm, and most preferably 52 cm. The plant spacing of the atriplex canescens is preferably 26-29 cm, more preferably 27-28 cm, and most preferably 27.5 cm. The row spacing of the sesbania is preferably 30-35 cm, more preferably 31-34 cm, and most preferably 33 cm. The present invention is preferably covered with mulching film after sowing. The width of the mulching film is preferably 100-150 cm, more preferably 120-130 cm, and most preferably 125 cm. The thickness of the floor film is preferably 0.005-0.007 mm, and more preferably 0.006 mm. After the mulching film is covered on the double ridges, the two sides of the mulching film are pressed into the soil and compacted to prevent water evaporation and salt from moving upwards.
After the seedlings of the atriplex canescens and sesbania are emerged, the thin film is poked at the seedling position in time, and the seedlings are ventilated. Thinning or replanting when the leaves of the atriplex canescens and the sesbania sessilifolia are 3-4, and setting 1 plant in each hole to ensure that the planting density of the atriplex canescens and the sesbania sessilifolia can be reached. Weeding is carried out in time when the leaves are 6-8 leaves, and the normal growth of the atriplex canescens and the sesbania is ensured.
The corn potentiators of the present invention are described in detail in the following examples, which should not be construed as limiting the scope of the present invention.
Example 1
By taking the planting of the atriplex canescens and sesbania intercropping in the heavy metal polluted saline-alkali soil in the new Tianjin coastal area as an example, the method for repairing the heavy metal polluted saline-alkali soil by planting the atriplex canescens and the sesbania in the coastal saline-alkali soil is explained.
The soil in the test area is salinized moist soil, the basic properties of the soil before improvement are that the total salt amount is 1.97g/kg, the pH value of the soil is 7.86, the organic matter content is 9.5g/kg, the alkaline hydrolysis nitrogen content is 82.5mg/kg, the effective phosphorus content is 8.63mg/kg, and the soil cadmium content is 2.56 mg/kg.
The crops to be tested were atriplex canescens and sesbania, and the seeds were provided by Jiangsu Yanchen Luyuan saline soil agricultural science and technology Co.
The field test is divided into four subareas, namely a test first area, a test second area, a test third area and a test fourth area, and the area of each subarea is 3.0m multiplied by 5.0 m.
In the first test area, the heavy metal polluted saline-alkali soil is not treated by applying an improver or trichoderma virens. When the single crop of the atriplex canescens is used, the planting distance of the atriplex canescens is 25cm, the row spacing is 60cm, and 4 rows are planted in total; when the sesbania is singly planted, the planting distance of the sesbania is 30cm, the row spacing is 60cm, and 4 rows are planted; the atriplex canescens and sesbania are intercropped as shown in figure 1, the row ratio of the atriplex canescens to the sesbania canescens of the atriplex canescens II (the II represents the atriplex canescens and the sesbania intercropping) is 1:2, namely 2 rows of sesbania are intercropped between every 1 row of the atriplex canescens, the row spacing of two adjacent rows of the atriplex canescens and the sesbania canescens is 60cm, the distance between two adjacent rows of the sesbania canescens is 60cm, the plant spacing of the atriplex canescens is.
And the second test area is a heavy metal polluted saline-alkali soil treatment area in which the modifier is applied and the trichoderma virens microbial inoculum is not applied, and the crop cultivation mode is the same as that of the first test area. In winter of the previous year of crop planting, phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure are used as modifiers to be applied to the soil of the heavy metal polluted saline-alkali soil, and then the upturned soil is frozen. The application amount of the modifier in the test is as follows: 200 kg/mu of phosphogypsum; 75 kg/mu of calcium superphosphate; 500 kg/mu of decomposed livestock and poultry manure. Uniformly spreading phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure on the surface of the soil layer, spraying bamboo vinegar liquid according to 0.5% of a solvent of an improvement dosage, and mechanically turning deeply for 40cm to uniformly mix the improver and the soil.
The three experimental areas are the treatment without applying modifying agents and applying trichoderma virens on the heavy metal polluted saline-alkali soil, and the crop cultivation mode is the same as that in the first experimental area. The application time of the trichoderma virens is when soil is prepared by spring sowing before sowing. Before sowing, water is drained to submerge the ground surface, after soaking for 6 hours, water is drained, trichoderma viride F7 solid microbial inoculum is uniformly spread on the surface layer of the soil according to the application amount of 30 kg/mu, and the soil is ploughed.
The preparation method of the trichoderma virens F7 solid microbial inoculum comprises the following steps: a. preparing spore suspension: inoculating activated Trichoderma viride F7 strain to PDA slant culture medium, culturing at 30 deg.C for 6 days, washing spores with sterile water, and diluting the spore solution to 1 × 106CFU/mL was used as spore suspension. b. Preparing a seed solution: inoculating the spore suspension into PDA liquid culture medium under aseptic condition, and culturing at 30 deg.C and 180rpm for 72h to obtain seed solution. c. Preparing a solid preparation: inoculating seed solution into a fermentation tank containing high-temperature sterilized solid fermentation culture medium according to the inoculation amount of 10%, introducing air at 30 deg.C for 6d to obtain Trichoderma viride F7 solid microbial inoculum, mixing, and counting the number of spores to 5 × 109Spores per gram.
The PDA slant culture medium comprises the following components: 200g of potato, 20g of glucose, 20g of agar and 1000mL of water, and the pH value is natural. The solid fermentation medium comprises the following components: 30g of wheat bran, 20g of rice bran, 10g of corn flour, 5g of soybean meal, 5g of glucose, 3.0g of monopotassium phosphate, 1.5g of calcium sulfate, 1.5g of magnesium sulfate and 1000mL of water.
The fourth test area is a treatment of applying the modifying agent and applying the trichoderma virens microbial inoculum on the heavy metal polluted saline-alkali soil, the crop cultivation mode is the same as the first test area, the modifying agent application is the same as the second test area, and the trichoderma virens microbial inoculum application is the same as the third test area.
The test totaled 12 treatments, each treatment was repeated 3 times. The experimental treatment is specified in table 1.
Table 1 test design table
A plough layer soil sample of 0-15 cm is taken at 20 days 4 months in 2010, the basic properties of the soil are measured, and the content of the biological available state of heavy metal cadmium in the soil is measured by adopting a gradient thin film diffusion technology (DGT) (see table 1).
Sowing atriplex canescens and sesbania, wherein the sowing date is 4 months and 25 days 2010, disinfecting seeds with 5% sodium hypochlorite for 30min before sowing, washing with tap water, and soaking in 7.5mmol/L gamma-aminobutyric acid for 8 h. During sowing, a sowing machine is used for ditching, manual dibbling or hole sowing is carried out along furrows, the sowing depth is 4cm, 5 seeds are planted in each hole, and the seeds are covered with soil, raked and treaded. Immediately after sowing, the film is covered to prevent water evaporation and salt from moving upwards.
Field management: after the seedlings of the atriplex canescens and the sesbania are emerged, the thin film is poked at the seedling position in time to blow the seedlings. Thinning or replanting is carried out when the leaves of the atriplex canescens and the sesbania are 3 leaves, and 1 plant is fixed in each hole, so that the planting density of the atriplex canescens and the sesbania can be ensured. Weeding is carried out in time when the leaves are 7 leaves, and the normal growth of the atriplex canescens and the sesbania is ensured.
Harvesting: plants were sampled 10 months and 12 days after the atriplex canescens and sesbania had matured. The sampling method comprises the steps of cutting off plants at a position 2.5cm close to the ground by using a sickle, respectively carrying out whole plant collection on crops, and then measuring the cadmium content and the biomass of the underground parts of the crops.
Test results
TABLE 2 basic properties of soil under different treatments
As can be seen from Table 2, the pH value of the soil layer of 0-15 cm without the modifier and the trichoderma virens agent is 9.76, the pH value of the soil treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent is obviously reduced, and the reduction amplitude is 0.42-0.92. Soil nutrients are obviously improved under the treatment of the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent, wherein the combined treatment (the four test areas) of the modifier and the trichoderma virens agent has the most obvious effect, and compared with the treatment (the first test area) without the modifier and the trichoderma virens agent, the contents of soil organic matters, alkaline hydrolysis nitrogen, available phosphorus and available potassium are respectively improved by 63.0 percent, 93.7 percent and 71.5 percent. The biological effective cadmium content in the soil is obviously improved by the treatment of the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent, and compared with the soil which is not treated by the modifier and the trichoderma virens agent (test area I), the biological effective cadmium content in the soil treated by the modifier (test area II), the trichoderma virens agent (test area III) and the modifier and the trichoderma virens agent (test area IV) is respectively improved by 30.4%, 42.0% and 75.9%.
TABLE 3 aerial and underground biomass (g/strain) of atriplex and sesbania
The results in Table 3 show that the application of the modifier and the Trichoderma viride preparation can obviously improve the biomass of atriplex canescens and sesbania sessilifolia in the same planting mode, and the combined effect of the two is the best. Under the single-crop mode of the atriplex canescens, compared with a control group, the biomass of the aboveground part and the underground part of the atriplex canescens treated by the modifying agent, the trichoderma virens agent, the modifying agent and the trichoderma virens agent are respectively increased by 8.70%, 7.66%, 12.9% and 11.7%, 8.42% and 15.8%; under the atriplex canescens sesbania intercropping mode, compared with a control group, the biomass of the aboveground part and the underground part of the atriplex canescens treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively increased by 12.4%, 8.84%, 14.4% and 13.6%, 9.89% and 19.5%; under the single-working mode of the sesbania, compared with a control, the biomass of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively increased by 6.97%, 6.60%, 14.0% and 10.1%, 8.47% and 15.9%; in the intercropping mode of the atriplex canescens sesbania, compared with a control, the biomass of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively increased by 8.61%, 7.59%, 15.5% and 11.4%, 9.45% and 16.9%. Under the same treatment, the biomass of the aboveground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively increased by 11.1 to 14.9 percent and 11.6 to 15.5 percent compared with the biomass of the aboveground part and the underground part of the atriplex canescens II sesbania is respectively increased by 11.0 to 13.8 percent and 16.9 to 18.9 percent compared with the biomass of the single atriplex canbania, which shows that the intercropping advantage is exerted by crop intercropping, and the biomass of the intercropping crops is obviously increased.
TABLE 4 cadmium content (mg/kg) in the aerial and underground parts of atriplex and sesbania
The results in Table 4 show that the application of the modifier and the Trichoderma viride preparation can significantly improve the cadmium content of the atriplex canescens and the sesbania sessilifolia in the same planting mode, and the combined action effect of the two is the best. In the single-crop mode of the atriplex canescens, compared with a control, the cadmium content of the overground part and the underground part of the atriplex canescens treated by the modifying agent, the trichoderma virens agent, the modifying agent and the trichoderma virens agent is respectively increased by 4.98%, 12.4%, 18.9%, 7.24%, 11.3% and 21.7%; in the atriplex II sesbania mode, compared with a control, the cadmium content of the overground part and the underground part of the atriplex treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent is respectively increased by 6.21%, 11.5%, 18.6% and 9.64%, 13.5% and 24.5%; in the sesbania single-working mode, compared with a control, the cadmium content of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent is respectively increased by 5.17%, 6.90%, 12.1%, 5.30%, 9.93% and 13.9%; in the atriplex II sesbania mode, the cadmium content of the overground part and the underground part of sesbania treated by the modifier, the trichoderma virens agent and the modifier + trichoderma virens agent is respectively increased by 5.85%, 7.98%, 16.0% and 4.92%, 12.0% and 19.0% compared with the control. Under the same treatment, the cadmium content of the overground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively improved by 6.90-11.8 percent and 7.23-12.5 percent compared with the cadmium content of the simple plant of the atriplex canescens II sesbania, and the cadmium content of the overground part and the underground part of the atriplex canbania is respectively improved by 6.90-11.8 percent and 7.23-12.5 percent compared with the cadmium content of the simple plant of the sesbania canescens II sesbania, which shows that the intercropping advantage is played in crop intercropping, and the cadmium.
TABLE 5 atriplex and sesbania aerial and underground cadmium content (μ g/strain)
The results in Table 5 show that the total cadmium accumulation of the atriplex canescens and the sesbania sessilifolia can be obviously improved by applying the modifying agent and the trichoderma virens preparation under the same planting mode, and the combined action effect of the modifying agent and the sesbania canescens is the best. In the single-crop mode of the atriplex canescens, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the atriplex canescens treated by the modifying agent, the trichoderma virens agent, the modifying agent and the trichoderma virens agent are respectively improved by 14.1%, 21.0%, 34.2% and 19.3%, 21.8% and 38.1%; in the atriplex II sesbania mode, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the atriplex treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively improved by 20.5%, 21.2%, 39.2% and 24.6%, 24.9% and 49.0%; under the single-working mode of the sesbania, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively increased by 12.5%, 13.3%, 27.9% and 15.7%, 18.9% and 31.8%; in the atriplex II sesbania mode, the total cadmium accumulation of the overground part and the underground part of sesbania treated by the modifier, the trichoderma virens agent and the modifier plus the trichoderma virens agent is respectively increased by 16.2%, 17.4%, 35.3% and 16.9%, 22.6% and 39.2% compared with the control. Under the same treatment, the total cadmium accumulation amount of the overground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively increased by 22.2-29.1 percent and 20.7-30.2 percent compared with the atriplex canescens single crop, and the total cadmium accumulation amount of the overground part and the underground part of the atriplex canescens II sesbania is respectively increased by 18.7-25.6 percent and 25.8-32.9 percent compared with the atriplex canbania single crop, which indicates that the intercropping advantage is played in crop intercropping and the total cadmium accumulation amount of the intercropping crops is obviously increased.
Example 2
By taking the planting of the atriplex canescens and sesbania intercropping in the heavy metal polluted saline-alkali soil in the new Tianjin coastal area as an example, the method for repairing the heavy metal polluted saline-alkali soil by planting the atriplex canescens and the sesbania in the coastal saline-alkali soil is explained.
The crops to be tested were atriplex canescens and sesbania, and the seeds were provided by Jiangsu Yanchen Luyuan saline soil agricultural science and technology Co.
The field test is divided into four subareas, namely a test first area, a test second area, a test third area, a test fourth area and the like, and the area of each subarea is 3.0m multiplied by 5.0 m.
In the first test area, the heavy metal polluted saline-alkali soil is not treated by applying an improver or trichoderma virens. When the single crop of the atriplex canescens is used, the planting distance of the atriplex canescens is 30cm, the row spacing is 50cm, and 4 rows are planted in total; when the sesbania is singly planted, the planting distance of the sesbania is 30cm, the row spacing is 50cm, and 4 rows are planted; the atriplex canescens and sesbania are intercropped as shown in figure 2, the row ratio of the atriplex canescens to the sesbania canescens of the atriplex canescens II (the II represents the atriplex canescens and the sesbania intercropping) is 1:2, namely 2 rows of sesbania are intercropped between every 1 row of the atriplex canescens, the row spacing of two adjacent rows of the atriplex canescens and the sesbania is 50cm, the row spacing of two adjacent rows of the sesbania is 50cm, and the.
And the second test area is a heavy metal polluted saline-alkali soil treatment area in which the modifier is applied and the trichoderma virens microbial inoculum is not applied, and the crop cultivation mode is the same as that of the first test area. In winter of the previous year of crop planting, phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure are used as modifiers to be applied to the soil of the heavy metal polluted saline-alkali soil, and then the upturned soil is frozen. The application amount of the modifier in the test is as follows: 300 kg/mu of phosphogypsum; 50 kg/mu of calcium superphosphate; 250 kg/mu of decomposed livestock and poultry manure. Uniformly spreading phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure on the surface of the soil layer, spraying bamboo vinegar liquid according to 1% of the solvent of the improvement dosage, and mechanically turning deeply for 40cm to uniformly mix the modifier with the soil.
The three experimental areas are the treatment without applying modifying agents and applying trichoderma virens on the heavy metal polluted saline-alkali soil, and the crop cultivation mode is the same as that in the first experimental area. The application time of the trichoderma virens is when soil is prepared by spring sowing before sowing. Before sowing, draining water to submerge the ground surface, soaking for 5-8 h, draining the water, uniformly spreading the trichoderma viride F7 solid microbial inoculum on the surface layer of the soil according to the application amount of 50 kg/mu, and ploughing into the soil.
The Trichoderma viride F7 is classified and named as Trichoderma virens, and is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No. 3.17613.
The preparation method of the trichoderma virens F7 solid microbial inoculum comprises the following steps: a. preparing spore suspension: inoculating activated Trichoderma viride F7 strain to PDA slant culture medium, culturing at 30 deg.C for 6 days, washing spores with sterile water, and diluting the spore solution to 1 × 106CFU/mL was used as spore suspension. b. Preparing a seed solution: inoculating the spore suspension into PDA liquid culture medium under aseptic condition, and culturing at 30 deg.C and 180rpm for 72h to obtain seed solution. c. Preparing a solid preparation: inoculating seed solution into a fermentation tank containing high-temperature sterilized solid fermentation culture medium according to the inoculation amount of 10%, introducing air at 30 deg.C for 6d to obtain Trichoderma viride F7 solid microbial inoculum, mixing, and counting the number of spores to 6 × 108Spores per gram. The trichoderma virens F7 can effectively reduce the pH value of saline-alkali soil and improve the effective state content of heavy metal in the soil, thereby playing a role in activating the heavy metal.
The PDA slant culture medium comprises the following components: 200g of potato, 20g of glucose, 20g of agar and 1000mL of water, and the pH value is natural. The solid fermentation medium comprises the following components: 30g of wheat bran, 20g of rice bran, 10g of corn flour, 5g of soybean meal, 5g of glucose, 3.0g of monopotassium phosphate, 1.5g of calcium sulfate, 1.5g of magnesium sulfate and 1000mL of water.
The fourth test area is a treatment of applying the modifying agent and applying the trichoderma virens microbial inoculum on the heavy metal polluted saline-alkali soil, the crop cultivation mode is the same as the first test area, the modifying agent application is the same as the second test area, and the trichoderma virens microbial inoculum application is the same as the third test area.
The test totaled 12 treatments, each treatment was repeated 3 times. The experimental treatment was the same as in example 1, and is shown in Table 1.
Sowing atriplex canescens and sesbania, wherein the sowing date is 4 months and 28 days 2010, disinfecting seeds with 5% sodium hypochlorite for 30min before sowing, washing with tap water, and soaking in 7.5mmol/L gamma-aminobutyric acid for 7 h. During sowing, a sowing machine is used for ditching, manual dibbling or hole sowing is carried out along furrows, the sowing depth is 3cm, 5 seeds are planted in each hole, and the seeds are covered with soil, raked and treaded. Immediately after sowing, the film is covered to prevent water evaporation and salt from moving upwards.
Field management: after the seedlings of the atriplex canescens and the sesbania are emerged, the thin film is poked at the seedling position in time to blow the seedlings. Thinning or replanting at 4 leaves of atriplex canescens and sesbania, and fixing 1 plant in each hole to ensure that the planting density of atriplex canescens and sesbania can be reached. Weeding is carried out in time when the leaves are 6 leaves, and the normal growth of the atriplex canescens and the sesbania is ensured.
Harvesting: and (4) taking a plant sample after the atriplex canescens and sesbania are mature for 10 months and 15 days. The sampling method comprises the steps of cutting off plants at a position 3cm close to the ground by using a sickle, respectively carrying out whole plant collection on crops, and then measuring the cadmium content and the biomass of the upper part and the lower part of the crops.
Test results
TABLE 6 atriplex and sesbania aerial and underground biomass (g/strain)
The results in Table 6 show that the application of the modifier and the Trichoderma viride preparation can obviously improve the biomass of atriplex canescens and sesbania sessilifolia in the same planting mode, and the combined effect of the two is the best. Under the single-crop mode of the atriplex canescens, compared with a control, the biomass of the aboveground part and the underground part of the atriplex canescens treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively improved by 9.88 percent, 9.08 percent, 15.1 percent, 9.43 percent, 8.96 percent and 19.3 percent; in the atriplex II sesbania mode, the biomass of the overground part and the underground part of the atriplex canescens treated by the modifier, the trichoderma virens inoculant and the modifier plus the trichoderma virens inoculant are respectively increased by 11.2%, 11.3%, 17.9% and 11.8%, 14.7% and 23.1% compared with a control; under the single-working mode of the sesbania, compared with a control, the biomass of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively increased by 7.30%, 6.45%, 15.1% and 11.5%, 12.6% and 22.5%; in the atriplex II sesbania mode, the aboveground and underground biomass of sesbania treated with modifier, Trichoderma virens, modifier + Trichoderma virens were increased by 9.31%, 8.24%, 16.3% and 11.5%, 12.6%, 22.5%, respectively, compared to the control. Under the same treatment, the biomass of the aboveground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively increased by 12.5 to 13.5 percent and 12.3 to 18.2 percent compared with the biomass of the aboveground part and the underground part of the atriplex canescens II sesbania is respectively increased by 11.2 to 13.3 percent and 18.0 to 22.9 percent compared with the biomass of the single atriplex canbania, which indicates that the intercropping advantage is exerted by crop intercropping, and the biomass of the intercropping crops is obviously increased.
TABLE 7 cadmium content (mg/kg) in the aerial and underground parts of atriplex and sesbania
The results in Table 7 show that the application of the modifier and the Trichoderma viride preparation can significantly improve the cadmium content of the atriplex canescens and the sesbania sessilifolia in the same planting mode, and the combined action effect of the two is the best. In the single-crop mode of the atriplex canescens, compared with a control, the cadmium content of the overground part and the underground part of the atriplex canescens treated by the modifying agent, the trichoderma virens agent, the modifying agent and the trichoderma virens agent is respectively increased by 5.36%, 14.3%, 19.2% and 9.12%, 13.4% and 20.4%; in the atriplex II sesbania mode, compared with a control, the cadmium content of the overground part and the underground part of the atriplex treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent is respectively improved by 9.47%, 14.4%, 22.6% and 9.98%, 13.5% and 27.4%; in the sesbania single-working mode, compared with a control, the cadmium content of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent is respectively increased by 6.03%, 7.54%, 13.6%, 7.44%, 11.6% and 14.0%; in the atriplex II sesbania mode, the cadmium content of the overground part and the underground part of sesbania treated by the modifier, the trichoderma virens agent and the modifier + trichoderma virens agent is respectively increased by 7.18%, 9.09%, 27.5% and 7.26%, 13.7% and 20.7% compared with the control. Under the same treatment, the cadmium content of the overground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively increased by 8.48 to 12.7 percent and 7.51 to 13.8 percent compared with the cadmium content of the single atriplex canescens, and the cadmium content of the overground part and the underground part of the atriplex canescens II sesbania is respectively increased by 5.03 to 13.3 percent and 6.37 to 12.8 percent compared with the cadmium content of the single sesbania canbania, which indicates that the intercropping advantage is exerted in crop intercropping and the cadmium content of the intercropping crops is obviously.
TABLE 8 atriplex and sesbania aerial and underground cadmium content (μ g/strain)
The results in Table 8 show that the total cadmium accumulation of atriplex canescens and sesbania can be remarkably improved by applying the modifying agent and the trichoderma virens preparation under the same planting mode, and the combined action effect of the modifying agent and the sesbania canescens is the best. In the single-crop mode of the atriplex canescens, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the atriplex canescens treated by the modifying agent, the trichoderma virens agent, the modifying agent and the trichoderma virens agent are respectively increased by 15.5%, 24.4%, 36.9% and 19.2%, 23.5% and 44.1%; in the atriplex II sesbania mode, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the atriplex treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively improved by 20.0 percent, 25.9 percent, 42.0 percent and 22.6 percent, 30.0 percent and 57.2 percent; in the sesbania single-crop mode, compared with a control, the total cadmium accumulation amounts of the overground part and the underground part of the sesbania treated by the modifier, the trichoderma virens agent, the modifier and the trichoderma virens agent are respectively improved by 13.7%, 14.5%, 30.8%, 18.9%, 21.3% and 36.6%; in the atriplex II sesbania mode, the total cadmium accumulation of the overground part and the underground part of sesbania treated by the modifier, the trichoderma virens agent and the modifier plus the trichoderma virens agent is respectively increased by 16.8 percent, 18.3 percent, 42.3 percent and 19.6 percent, 27.9 percent and 47.7 percent compared with a control. Under the same treatment, the total cadmium accumulation amount of the overground part and the underground part of the atriplex canescens in the atriplex canescens II sesbania is respectively increased by 22.0-26.8 percent and 20.6-31.6 percent compared with the atriplex canescens single crop, and the total cadmium accumulation amount of the overground part and the underground part of the atriplex canescens II sesbania is respectively increased by 17.1-27.5 percent and 26.4-36.7 percent compared with the atriplex canbania single crop, which indicates that the intercropping advantage is played in crop intercropping and the total cadmium accumulation amount of the intercropping crops is obviously increased.
The invention provides a saline-alkali soil remediation method, which utilizes the complementary advantages of a symbiotic system formed by atriplex canescens and sesbania intercropping, the improvement effect of a modifier and the growth promotion effect of trichoderma virens, and can obviously reduce the heavy metal pollution of saline-alkali soil and obviously improve the biomass of intercropped crops.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A saline-alkali soil remediation method comprises the following steps:
1) in winter, applying the modifying agent into saline-alkali soil, spraying bamboo vinegar, and ploughing; the mass of the bamboo vinegar liquid is 0.5-1% of that of the modifying agent;
the saline-alkali soil is polluted by heavy metal;
the active ingredients of the modifier are phosphogypsum, calcium superphosphate and decomposed livestock and poultry manure;
2) in the spring of the next year, applying trichoderma virens into the ploughed soil; planting atriplex canescens and sesbania in an intercropping way; before planting, the method further comprises the step of disinfecting seeds of the atriplex canescens and the sesbania sessilifolia, wherein the disinfection is carried out for 25-35 min by adopting sodium hypochlorite with the mass-volume ratio of 4-6%, and the seeds are soaked in gamma-aminobutyric acid with the concentration of 6-8 mmol/L for 6-10 h after being washed by tap water.
2. The repair method of claim 1, wherein the effective components of the modifying agent in the step 1) comprise the following components in parts by weight: 4-6 parts of phosphogypsum, 1-1.5 parts of calcium superphosphate and 5-10 parts of decomposed livestock and poultry manure.
3. Repair method according to claim 2, characterized in that the CaSO in phosphogypsum4·2H2The O content is not lower than 90%, and the application amount is 200-300 kg/mu; the calcium superphosphate contains not less than 11% of Ca, not less than 14% of phosphorus and the application amount is 50-75 kg/mu; the organic matter content of the decomposed livestock and poultry manure is not lower than 30g/kg, and the application amount is 250-500 kg/mu.
4. The method for repairing according to claim 3, wherein the method for preparing decomposed livestock and poultry manure comprises: mixing livestock and poultry manure, urea, calcium superphosphate and premix to obtain a mixture, adjusting the water content of the mixture to be 45-55%, and fermenting for 21-28 days; the premix comprises 0.5-1.5 parts of quick-rotting agent, 15-20 parts of bran and 20-30 parts of corn flour.
5. The rehabilitation method according to claim 4, wherein the pile is turned over every 7 to 10 days during the fermentation process.
6. The repair method according to claim 1, wherein the trichoderma virens inoculum of step 2) comprises a trichoderma virens F7 strain, wherein the trichoderma virens F7 strain has a collection number of CGMCC No. 3.17613; the spore amount in the microbial inoculum is 6 multiplied by 108~5×109Per gram.
7. The restoration method according to claim 1, wherein the application amount of the microbial inoculum in step 2) is 30-50 kg/mu.
8. The repairing method according to claim 1, wherein the canes and sesbania are planted in the intercropping way in the step 2), every two rows of the canes comprise 1-3 rows of the sesbania, the distance between every two adjacent rows of the canes and the sesbania is 45-60 cm, the distance between every two adjacent rows of the sesbania is 45-60 cm, the planting distance of the canes is 25-30 cm, and the planting distance of the sesbania is 30-35 cm.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222387A (en) * | 2013-05-21 | 2013-07-31 | 中国科学院遗传与发育生物学研究所 | Method suitable for intercropping sorgho and sesbania on saline and alkaline land |
| CN105344708A (en) * | 2015-09-30 | 2016-02-24 | 河南行知专利服务有限公司 | Remediation method for heavy metal contaminated soil of saline and alkaline land |
| CN106424126A (en) * | 2016-09-05 | 2017-02-22 | 青岛农业大学 | Remediation method for copper and cadmium co-contaminated soil |
| CN106947716A (en) * | 2017-03-31 | 2017-07-14 | 上海立昌环境工程股份有限公司 | The agent of heavy metal pollution Saline-alkali Field Control and saline-alkali soil treatment method comprising spent bleaching clay |
| CN109197008A (en) * | 2018-10-18 | 2019-01-15 | 北京市园林绿化集团有限公司 | A method of administering improvement salt-soda soil |
| CN109354556A (en) * | 2018-12-05 | 2019-02-19 | 兴安盟博川农业科技有限公司 | A kind of composite conditioner and the preparation method and application thereof for improveing alkaline land soil |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641752B (en) * | 2015-01-05 | 2016-05-11 | 山西省农业科学院农业环境与资源研究所 | The method in jerusalem artichoke intercropping oat improvement salt-soda soil, inland |
| CN104885741B (en) * | 2015-05-18 | 2017-04-26 | 甘肃农业大学 | Method for biologically improving hardened and salinized soil through intercropping of sainfoin and four-wing saltbush |
| CN105794484A (en) * | 2016-05-10 | 2016-07-27 | 连云港市农业科学院 | No-tillage planting method of tomato in northern Suzhou intertidal zone saline-alkali soil |
| CN106305080A (en) * | 2016-08-23 | 2017-01-11 | 陈丁龙 | Method for planting winter jujube by restoring saline-alkali land |
| CN106305087A (en) * | 2016-08-23 | 2017-01-11 | 陈丁龙 | Method for planting apocynum venetum by soil restoration of saline-alkali land |
| CN107030104B (en) * | 2017-06-14 | 2020-04-17 | 青岛农业大学 | Method for jointly repairing heavy metal contaminated soil by using trichoderma virens and suaeda salsa |
| CN107815315A (en) * | 2017-10-20 | 2018-03-20 | 福建省农业科学院农业生态研究所 | A kind of soil conditioner, its preparation method and application for improving saline-alkali soil |
| CN109169067A (en) * | 2018-09-30 | 2019-01-11 | 湖南博川农业发展有限责任公司 | A kind of implantation methods made between corn and sesbania for salt-soda soil |
-
2019
- 2019-03-08 CN CN201910176612.9A patent/CN109759441B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222387A (en) * | 2013-05-21 | 2013-07-31 | 中国科学院遗传与发育生物学研究所 | Method suitable for intercropping sorgho and sesbania on saline and alkaline land |
| CN105344708A (en) * | 2015-09-30 | 2016-02-24 | 河南行知专利服务有限公司 | Remediation method for heavy metal contaminated soil of saline and alkaline land |
| CN106424126A (en) * | 2016-09-05 | 2017-02-22 | 青岛农业大学 | Remediation method for copper and cadmium co-contaminated soil |
| CN106947716A (en) * | 2017-03-31 | 2017-07-14 | 上海立昌环境工程股份有限公司 | The agent of heavy metal pollution Saline-alkali Field Control and saline-alkali soil treatment method comprising spent bleaching clay |
| CN109197008A (en) * | 2018-10-18 | 2019-01-15 | 北京市园林绿化集团有限公司 | A method of administering improvement salt-soda soil |
| CN109354556A (en) * | 2018-12-05 | 2019-02-19 | 兴安盟博川农业科技有限公司 | A kind of composite conditioner and the preparation method and application thereof for improveing alkaline land soil |
Non-Patent Citations (1)
| Title |
|---|
| γ氨基丁酸浸种对高氮处理下白菜生长及硝酸盐代谢的影响;李敬蕊等;《园艺学报》;20161130(第11期);第2182–2192页 * |
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