CN109759160A - A kind of biochip reagent quantitative Adding Way and system - Google Patents
A kind of biochip reagent quantitative Adding Way and system Download PDFInfo
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- CN109759160A CN109759160A CN201910286996.XA CN201910286996A CN109759160A CN 109759160 A CN109759160 A CN 109759160A CN 201910286996 A CN201910286996 A CN 201910286996A CN 109759160 A CN109759160 A CN 109759160A
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Abstract
The invention discloses a kind of biochip reagent quantitative Adding Ways and system, and wherein reagent quantitative Adding Way includes: that the plunger in displacement drive unit driving biochip reagent chamber is at the uniform velocity moved down with speed V1；During plunger at the uniform velocity moves down, the total quantity of the drop dripped from reagent chamber liquid droping port is calculated；When drop total quantity is equal to preset value, displacement drive unit stops driving plunger and moves down；When drop total quantity, which is equal to preset value, subtracts 1, displacement drive unit driving plunger is at the uniform velocity moved down with speed V2, and wherein V2 is less than or equal to V1；Before plunger at the uniform velocity moves down, cog region is demarcated below reagent chamber liquid droping port in advance；During plunger at the uniform velocity moves down, the image of cog region is obtained in real time, drips are determined whether there is according to the image recognition result of cog region.The present invention can accurately control be added reactor in amount of reagent, be quantitatively adding precision height, biochip is integrally required it is low, it is low in cost, it is easy to accomplish.
Description
Technical field
The invention belongs to biochip test field, in particular to a kind of biochip reagent quantitative Adding Way and it is
System.
Background technique
Reagent (sample) is quantitatively precisely the important prerequisite of all automated analysis detecting instrument output accurate results.Mesh
Before, automated analysis instrument realizes that reagent (sample) is quantitative generally by micro quantitative determination needle, precision pump or precision in the market
Motor driven is realized.The quantitative precision of reagent (sample) will directly influence the reproducibility and accuracy of test result.It is micro
The quantitative more small and exquisite precision of needle itself, expensive, lower instrument of ordinary circumstance can only be equipped with several；And with quantitatively
Needle draws different reagent (sample) and generally requires be repeated several times to wash needle operation, delays, also brings reagent (sample
Originally) the risk of cross contamination.Realize that reagent (sample) is quantitative high to required precision in such a way that precision electric motor controls stroke,
With high costs, speed is also slow, especially larger capacity liquid reagent to be quantitatively adding speed slower.Also there are many large sizes
Instrument and equipment realizes quantifying for micro liquid reagent (or sample) using precise injection pump, but this need to injection speed or
The stroke of person's syringe pump carries out accurate control.Also some forms fine droplet in exit to realize by syringe pump promotion liquid
It is quantitative.Its detailed process be the size of drop (diameter, radius etc.) is detected in the dosing process of micro liquid, if
It is not inconsistent with pre-set droplet size, then changes droplet size by adjusting the injection speed of syringe pump.It does so final
Though desired droplet size can be obtained, an adjustment period must be undergone, operation is corrected to the size of drop, then
The drop for exporting a certain size could be stablized.It, must be right if encountering the situation for needing to be quantitatively adding lot of trace reagent
Syringe pump and its pipeline are cleaned, or use more set equipment；The former expends the time at cleaning process, and changes different reagents
After need to be corrected droplet size adjustment, and the latter is because syringe pump is with high costs, then very uneconomical using more set equipment.This
Outside, syringe pump is expensive, and volume is relatively large, generally is relatively suitable for use in being worth on higher large-scale instrument.
The microflow controlled biochip that developed recently gets up is as medical disposable material, small volume, the basic nothing in inner space
Method places the biggish precise part of the such volume of syringe pump, and chip is disposably, will not to reuse, also not permit in cost
Perhaps.Therefore, inside biochip, reagent (sample) can not quantitatively be realized by way of inside plus pump, need to develop it
It is easy to operate, is easily achieved and cheap reagent quantitative new technology.
Summary of the invention
It is an object of the present invention in view of the above shortcomings of the prior art, a kind of biochip is provided and is added with reagent quantitative
Enter method and system, can not only accurately control the amount that each reagent in disposable chip reactor is added, and reagent quantitative adds
Enter precision height, biochip integrally required low, structure is simple, low in cost, it is easy to accomplish, be conducive to biochip test skill
The development of art.
In order to solve the above technical problems, the technical scheme adopted by the invention is that:
A kind of biochip reagent quantitative Adding Way, comprising:
Plunger in displacement drive unit driving biochip reagent chamber is at the uniform velocity moved down with speed V1；
Its main feature is that: during plunger at the uniform velocity moves down, calculate the total quantity of the drop dripped from reagent chamber liquid droping port；When
When drop total quantity is equal to preset value, displacement drive unit stops driving plunger and moves down.
Further, when drop total quantity be equal to preset value subtract 1 when, displacement drive unit drive plunger with speed V2 at the uniform velocity
It moves down, wherein V2 is less than or equal to V1.
As a preferred method, before plunger at the uniform velocity moves down, demarcates know below reagent chamber liquid droping port in advance
Other area；During plunger at the uniform velocity moves down, the image of cog region is obtained in real time, according to the image recognition result of cog region
Determine whether there is drips.
Become larger again when the gray value of image of cog region experienced from small from the process to become smaller greatly as a preferred method,
When, then it is determined with a drips.
Further, when drop total quantity is equal to preset value, displacement drive unit stops driving plunger and moves down；Together
When, displacement drive unit drives plunger to move up a distance along reagent cavity axis.
Preset value is determined by following methods as a preferred method:
Firstly, production reagent chamber, when the plunger in displacement drive unit driving biochip reagent chamber is at the uniform velocity downward with speed V1
When mobile, the volume m for the single dropping liquid drop dripped from reagent chamber drop mouth is calculated；
Then, with the destination agent amount being added is needed divided by m, obtained quotient, which is rounded, obtains drop preset value；Wherein, m is less than
Or the destination agent amount being added equal to needs.
Based on the same inventive concept, the present invention also provides a kind of biochip reagent quantitatives, and system, biology is added
Plunger is equipped in the reagent chamber of chip, displacement drive unit is connected with plunger actuation, its main feature is that further including controller and being used for
The counter of the drop sum dripped from reagent chamber liquid droping port is calculated, the output end of counter and the input terminal of controller are electrically connected
It connects, the output end of controller is electrically connected with the control terminal of displacement drive unit, wherein
Displacement drive unit: for driving plunger axially at the uniform velocity to move along reagent chamber according to the control instruction of controller；
Controller: it is axially at the uniform velocity moved to displacement drive unit with controlling plunger along reagent chamber for issuing control instruction；It is used in combination
In the detection data of count pick up device, and when the drop total quantity that counter detects is equal to preset value, control instruction is issued
It is moved down to displacement drive unit with controlling plunger stopping；
Preset value is preset in controller.
Further, controller is also used to issue control when the drop total quantity that counter detects is equal to preset value and subtracts 1
System instruction is at the uniform velocity moved down to displacement drive unit with controlling plunger with speed V2, and wherein V2 is less than or equal to the initial of plunger
Speed V1.
The counter is imaging identification unit, the output end of imaging identification unit and control as a preferred method,
The input terminal of device is electrically connected；Calibration has cog region below reagent chamber liquid droping port, wherein
Imaging identification unit: determine whether for obtaining the image of cog region in real time, and according to the image recognition result of cog region
There are drips, to calculate the total quantity of the drop dripped from reagent chamber liquid droping port；
Control unit: the drop total quantity for being detected according to imaging identification unit issues control instruction to displacement drive list
Member.
Become larger as a preferred method, when imaging identification unit detects that the gray value of image of cog region experienced from small
When again from the process to become smaller greatly, then a drips are determined with.
Further, control unit be also used to drop total quantity be equal to preset value when, issue control instruction to be displaced drive
Moving cell is to drive plunger to move up a distance along reagent cavity axis.
Be found through experiments that, if on disposable biological chip liquid droping port geomery (such as wall thickness) once it is determined that, it is right
For same liquid reagent, in the case where oozing speed and being not much different, droplet size substantially constant, as long as drop number control is quasi-
The reagent (sample) that really can be achieved with very high degree of precision is quantitatively adding and (the CV value of test is repeated several times less than 2%).
As a preferred method, in controller preset value determination method are as follows: firstly, production reagent chamber, when displacement drive
When plunger in moving cell driving biochip reagent chamber is at the uniform velocity moved down with speed V1, calculates and dripped from reagent chamber drop mouth
Single dropping liquid drop volume m；Then, with the destination agent amount being added is needed divided by m, it is default that obtained quotient's rounding obtains drop
Value；Wherein, m is less than or equal to the destination agent amount for needing to be added.
Compared with prior art, the present invention is identified and is counted by the drop dripped to reagent chamber liquid droping port, is being reached
The stopping of feedback control plunger moves down when to default drop number, so that accurately the amount of the reagent in reactor, reagent is added in control
It is quantitatively adding precision height, it is not high to the required precision of displacement driving unit (such as stepper motor), to chip interior micro fluid circuits shape
Shape and coherence request be not high, only has higher requirements to the consistency of reagent chamber liquid droping port, and reagent chamber liquid droping port can use mould
Tool realizes batch machining easily, and consistency is good, and low in cost, it is easy to accomplish, be conducive to the development of biological chip testing technology.
Detailed description of the invention
Fig. 1 is one embodiment flow chart of biochip reagent quantitative Adding Way of the present invention.
Fig. 2 is that one example structure schematic diagram of system is added in biochip reagent quantitative of the present invention.
Fig. 3 is state change schematic diagram when drop passes through cog region.
Wherein, 1 is reagent chamber, and 101 be cog region, and 2 be plunger, and 3 be displacement drive unit, and 301 be electric-motor drive unit,
302 be stepper motor, and 4 be controller, and 5 be counter, and 6 be light source, and 7 be reactor.
Specific embodiment
As shown in Figure 1, biochip reagent quantitative Adding Way includes:
Displacement drive unit 3 drives the plunger 2 in biochip reagent chamber 1 at the uniform velocity to move down with speed V1；
During plunger 2 at the uniform velocity moves down, the total quantity of the drop dripped from 1 liquid droping port of reagent chamber is calculated；When drop is total
When quantity is equal to preset value, displacement drive unit 3 stops driving plunger 2 and moves down；Wherein, preset value is true by following methods
It is fixed:
Firstly, being directed to the dosage and its property of variety classes reagent, set in such a way that theoretical calculation and experimental verification combine
The liquid droping port in meter production reagent chamber 1 and its exit, to obtain the reagent droplet of set size.When displacement drive unit 3 drives
When plunger 2 in biochip reagent chamber 1 is at the uniform velocity moved down with speed V1, the single dropping liquid dripped from 1 liquid droping port of reagent chamber drips
Volume be m, obtain the size of m.Wherein, the property of the numerical values recited of m and reagent itself, the size of 1 liquid droping port of reagent chamber are (interior
Diameter, wall thickness etc.) it is related；For reagent of the same race, as long as reagent chamber 1 and its size of liquid droping port are consistent, then in the shifting of plunger 2
Under conditions of dynamic similar rate, the numerical value substantially constant of m.
Then, with the destination agent amount being added is needed, divided by m, obtained in close proximity to one integer of quotient is rounded to obtain the final product
To drop preset value；Wherein, m is less than or equal to the destination agent amount for needing to be added.
When drop total quantity, which is equal to preset value, subtracts 1, displacement drive unit 3 drives plunger 2 with speed V2 at the uniform velocity to moving down
Dynamic, wherein V2 is less than or equal to V1, to guarantee residual at liquid droping port when last dropping liquid is dripped and oozed from 1 liquid droping port of reagent chamber
Stay it is less, enable plunger 2 retract playback when the reagent remained at liquid droping port is sucked back.
In the present invention, it is desirable that V1 and V2 value enable to drop to ooze speed slower, it has to be possible to the shape at liquid droping port
At apparent drop, so that drop oozes speed no more than 3 drops/sec from liquid droping port.If V1 or V2 value is excessive, cause
It is more than 3 drops/sec that liquid droping port drop, which oozes speed, may identify to drop and cause difficulty.
Before plunger 2 at the uniform velocity moves down, cog region 101 is demarcated below 1 liquid droping port of reagent chamber in advance；In plunger 2
During at the uniform velocity moving down, the image of cog region 101 is obtained in real time, is determined according to the image recognition result of cog region 101
Whether drips are had.
When due to liquid drippage, the variation of image grayscale feature of cog region 101 be will be by secretly brightening, then by bright dimmed,
Thus when the gray value of image of cog region 101 experienced from small become larger again from the process to become smaller greatly, then drop drop is determined with
It falls.
When drop total quantity is equal to preset value, displacement drive unit 3 stops driving plunger 2 and moves down；Meanwhile it being displaced
Driving unit 3 drives plunger 2 to move up a distance along 1 axis of reagent chamber.
As shown in Fig. 2, biochip is equipped with plunger 2 in reagent quantitative addition system in the reagent chamber 1 of biochip,
Displacement drive unit 3 is connected with the driving of plunger 2, its main feature is that further including controller 4 and dripping for calculating from 1 liquid droping port of reagent chamber
The output end of the counter 5 of the drop sum fallen, counter 5 is electrically connected with the input terminal of controller 4, the output end of controller 4
It is electrically connected with the control terminal of displacement drive unit 3, wherein
Displacement drive unit 3: for driving plunger 2 axially at the uniform velocity to move along reagent chamber 1 according to the control instruction of controller 4；
Controller 4: it is axially at the uniform velocity moved to displacement drive unit 3 with controlling plunger 2 along reagent chamber 1 for issuing control instruction；
And it is used for the detection data of count pick up device 5, and when the drop total quantity that counter 5 detects is equal to preset value, issue control
System instruction controls the stopping of plunger 2 to displacement drive unit 3 and moves down；
Preset value is preset in controller 4, wherein preset value determines method are as follows: firstly, production reagent chamber 1, when displacement drive list
When member 3 drives the plunger 2 in biochip reagent chamber 1 at the uniform velocity to move down with speed V1, calculates and dripped from 1 drop mouth of reagent chamber
Single dropping liquid drop volume m；Then, with the destination agent amount being added is needed divided by m, it is default that obtained quotient's rounding obtains drop
Value；Wherein, m is less than or equal to the destination agent amount for needing to be added.
Controller 4 is also used to issue control instruction when the drop total quantity that counter 5 detects is equal to preset value and subtracts 1
It is at the uniform velocity moved down to displacement drive unit 3 with controlling plunger 2 with speed V2, wherein V2 is less than or equal to the initial speed of plunger 2
Spend V1.
The counter 5 is imaging identification unit, and the input terminal of the output end and controller 4 of imaging identification unit is electrically connected
It connects；Calibration has cog region 101 below 1 liquid droping port of reagent chamber, wherein
Imaging identification unit: sentence for obtaining the image of cog region 101 in real time, and according to the image recognition result of cog region 101
It is fixed whether to have drips, to calculate the total quantity of the drop dripped from 1 liquid droping port of reagent chamber；
Control unit: the drop total quantity for being detected according to imaging identification unit issues control instruction to displacement drive unit
3。
Become larger again when imaging identification unit detects that the gray value of image of cog region 101 experienced from small from the mistake to become smaller greatly
Cheng Shi is then determined with a drips.
State change of the drop by cog region when is shown in Fig. 3.
Control unit be also used to drop total quantity be equal to preset value when, issue control instruction to displacement drive unit 3 with
Plunger 2 is driven to move up a distance along 1 axis of reagent chamber.
Biochip reagent quantitative is added system and uses 24V DC power supply.
Displacement drive unit 3 includes electric-motor drive unit 301 and stepper motor 302, and the output end of controller 4 passes through motor
Driving unit 301 is electrically connected with the driving end of stepper motor 302, and the output end of stepper motor 302 is connected with the transmission of plunger 2.
It further includes the light source 6 matched with imaging identification unit that system, which is added, with reagent quantitative in biochip.
The driving end of the counter 5 and light source 6 is electrically connected with the output end of controller 4.
Using the present invention, as long as from the amount of droplets precise control that 1 liquid droping port of reagent chamber drips, then in droplet size
Under conditions of (volume) has high consistency, the precision that reactor 7 is added in reagent quantitative is very high, and reagent is repeatedly added in parallel
CV value even can achieve 2% or less.The present invention can be realized reagent quantitative and rapidly join, and at 1 liquid droping port of reagent chamber without
Residual.
In the present invention, the setting process of preset value is as follows: firstly, for selected 1 liquid droping port of reagent and reagent chamber, surveying
Determine the droplet size that reagent drips from 1 liquid droping port of reagent chamber and acquire drop preset value further according to the reagent total amount of demand,
Wherein drop preset value is equal to the reagent total amount of demand divided by the droplet size dripped at liquid droping port.
Can Multiple Type prefabricated first and specification as needed 1 liquid droping port of reagent chamber, to meet the needs of different.
It being found by test of many times, the method for the present invention is high to the quantitative accuracy of trace reagent, and it is reproducible, even for low
To the reagent of 10 μ L magnitudes, quantitative CV value, which is repeated several times, can also easily do 2% or less.And in the prior art, this field
The CV value that interior reagent quantitative is added is generally 5% ~ 10%.
Tables 1 and 2 realizes what trace reagent was quantitatively adding during showing chip actual test by the method for the invention
Example and accuracy data, weighing analysis the result shows that, with the method for the present invention by control reagent drip number be added trace reagent essence
It spends very high.
According to reagent dosage needed for inspection project, size design is carried out to 1 liquid droping port of reagent chamber, size conjunction can be obtained
Suitable reagent droplet finally only needs to carry out control to amount of droplets by the method for the invention that the high-precision of trace reagent can be realized
Degree is quantitatively adding.
If certain medical test project needs to use 3 kinds of reagents, the destination agent amount for intending being added is 50 μ L, then passing through
The reagent droplet size that design 1 liquid droping port of reagent chamber control oozes is 17 μ L or so, it is only necessary to which 3 drop reagents can meet 50 μ L
Volume needs.50 μ L or so above-mentioned 3 kinds of reagents are added to using the method for the present invention in certain verification test in R&D process
Precision is verified respectively, the results are shown in Table 1, the density of each true reagent is all close to water in table.
Seen from table 1, after design, the size that liquid droping port can control above-mentioned 3 kinds of reagent droplets is 17 ~ 18 μ L, is tired out
3 drop of product can meet the reagent dosage demand of 50 μ L or so.And the addition precision of each reagent is all very high, 15 chip difference
The whole CV value for repeating to be added dropwise is no more than 2%.
In addition, the method for the present invention is down to 10 quantifying for μ L magnitude trace reagent for standing good, and still can achieve
Very high precision.Table 2 lists 20 quantitative datas in one-time authentication experiment to certain 10 μ L magnitude reagent.It is adopted when quantitative
With the method for the present invention, dripping liquid droping port to control the reagent droplet size by reagent chamber 1 is 10 μ L or so, and 1 drop is added in every secondary control
?.The reagent density is close to water.
Know that 20 quantitative average values are 0.0105 g(≈, 10.5 μ L by calculating), and the CV that 20 repetitions are quantitative
Value is 1.70%, has high quantitative accuracy.
A kind of biochip provided by the present invention is had the advantage that with reagent quantitative Adding Way and system
(1) high to the quantitative accuracy of trace reagent.It is small (CV≤2%) that same reagent is repeated several times quantitative volumetric errors, is entirely capable of
Enough meet measuring accuracy requirement.
(2) it is suitble to disposable biological chip to use.In addition to chip itself is disposably, other matching components are all fixed
On instrument, only needs to change chip and can be realized the reagent on different chips is quantified.
(3) the expensive precise part using precise injection pump etc is not needed, it is low in cost.It only needs common using one
Motor can push chip reagent chamber plunger motion.
(4) very low to the stability requirement of liquid reagent flow velocity during reagent oozes, it is not needed at all by accurately adjusting
Whole and control (syringe pump) flow velocity controls droplet size.
(5) during drips, it is not relevant for the size of droplet size dripped in real time, it is thus only necessary to which concern is added
Amount of droplets.
(6) size of chip liquid droping port it has been pre-designed, it can be ensured that reagent of the same race is never the same as the corresponding liquid droping port of chip
Droplet size is to the extent permitted by the error when oozing.
There are essential distinctions with prior art for technical solution in the present invention.
Such as, CN1337572A discloses a kind of liquid drop analysis device, be to provide one kind can continuous or interruption measure
The optical imagery liquid drop analysis device based on droplet size of the instantaneous volume of drop, while the device can also be directly or indirectly
A variety of Wuli-Shili-Renli system approach parameters of liquid are measured simultaneously.To sum up, the liquid drop analysis device stress to the volume of drop and
Liquid other parameters are analyzed, and the present invention is quantifying for biochip inside trace reagent to be realized, does not need to be concerned about
The volume and liquid property of drop, it is only necessary to amount of droplets be counted, the two purpose and implementation have larger
Difference.
For another example, US4936828 discloses a kind of Liquid particle image sensor, and main purpose is still in order to measure the body of drop
Product, and during reagent quantitative is added, the present invention does not need measurement droplet size.
The present invention provides a kind of biochip reagent quantitative Adding Ways and system, by way of forming drop come
Realize reagent quantitative.Reagent quantitative process does not need to carry out analytical calculation to the volume of drop and other properties, it is only necessary to from
The reagent droplet quantity that biochip liquid droping port oozes, which carries out simple count, can be realized, and it is primary can not only accurately to control addition
Property biochip reaction device in each reagent amount, and to be added precision high for reagent quantitative, integrally requires biochip low, structure
It is simply, low in cost, it is easy to accomplish.
The embodiment of the present invention is described with above attached drawing, but the invention is not limited to above-mentioned specific
Embodiment, the above mentioned embodiment is only schematical, rather than limitation, those skilled in the art
Under the inspiration of the present invention, without breaking away from the scope protected by the purposes and claims of the present invention, it can also make very much
Form, within these are all belonged to the scope of protection of the present invention.
Claims (12)
1. a kind of biochip reagent quantitative Adding Way, comprising:
Plunger (2) in displacement drive unit (3) driving biochip reagent chamber (1) is at the uniform velocity moved down with speed V1；
It is characterized in that,
During plunger (2) at the uniform velocity moves down, the total quantity of the drop dripped from reagent chamber (1) liquid droping port is calculated；Work as liquid
When dripping total quantity equal to preset value, displacement drive unit (3) stops driving plunger (2) and moves down.
2. biochip as described in claim 1 reagent quantitative Adding Way, which is characterized in that when drop total quantity is equal to
When preset value subtracts 1, displacement drive unit (3) driving plunger (2) is at the uniform velocity moved down with speed V2, and wherein V2 is less than or equal to
V1。
3. biochip as claimed in claim 1 or 2 reagent quantitative Adding Way, which is characterized in that
Before plunger (2) at the uniform velocity moves down, cog region (101) are demarcated below reagent chamber (1) liquid droping port in advance；
During plunger (2) at the uniform velocity moves down, the image of cog region (101) is obtained in real time, according to cog region (101)
Image recognition result determines whether there is drips.
4. biochip as claimed in claim 3 reagent quantitative Adding Way, which is characterized in that when cog region (101)
Gray value of image experienced from it is small become larger again from the process to become smaller greatly when, then be determined with a drips.
5. biochip as described in claim 1 reagent quantitative Adding Way, which is characterized in that when drop total quantity is equal to
When preset value, displacement drive unit (3) stops driving plunger (2) and moves down；Meanwhile displacement drive unit (3) drives plunger
(2) along reagent chamber (1), axis moves up a distance.
6. biochip as described in claim 1 reagent quantitative Adding Way, which is characterized in that preset value is by following methods
It determines:
Firstly, production reagent chamber (1), when the plunger (2) in displacement drive unit (3) driving biochip reagent chamber (1) is with speed
When degree V1 is at the uniform velocity moved down, the volume m for the single dropping liquid drop dripped from reagent chamber (1) drop mouth is calculated；
Then, with the destination agent amount being added is needed divided by m, obtained quotient, which is rounded, obtains drop preset value；Wherein, m is less than
Or the destination agent amount being added equal to needs.
7. system is added in a kind of biochip reagent quantitative, plunger (2) are equipped in the reagent chamber (1) of biochip, displacement is driven
Moving cell (3) is connected with plunger (2) driving, which is characterized in that
Further include controller (4) and the counter (5) for calculating the drop sum dripped from reagent chamber (1) liquid droping port, counts
The output end of device (5) is electrically connected with the input terminal of controller (4), the output end of controller (4) and the control of displacement drive unit (3)
End electrical connection processed, wherein
Displacement drive unit (3): for driving plunger (2) axial at the uniform velocity along reagent chamber (1) according to the control instruction of controller (4)
It is mobile；
Controller (4): axial even along reagent chamber (1) to control plunger (2) to displacement drive unit (3) for issuing control instruction
Speed is mobile；And the drop total quantity for being used for the detection data of count pick up device (5), and detecting in counter (5) be equal to it is default
When value, sending control instruction controls plunger (2) to displacement drive unit (3) and stops moving down；
Preset value is preset in controller (4).
8. system is added in reagent quantitative as claimed in claim 7, which is characterized in that
Controller (4): it is also used to issue control instruction when the drop total quantity that counter (5) detects is equal to preset value and subtracts 1
It is at the uniform velocity moved down to displacement drive unit (3) with controlling plunger (2) with speed V2, wherein V2 is less than or equal to plunger (2)
Initial velocity V1.
9. system is added in reagent quantitative as claimed in claim 7 or 8, which is characterized in that the counter (5) is imaging identification
The output end of unit, imaging identification unit is electrically connected with the input terminal of controller (4)；There is calibration below reagent chamber (1) liquid droping port
Cog region (101), wherein
Imaging identification unit: for obtaining the image of cog region (101) in real time, and according to the image recognition knot of cog region (101)
Fruit determines whether there is drips, to calculate the total quantity of the drop dripped from reagent chamber (1) liquid droping port；
Control unit: the drop total quantity for being detected according to imaging identification unit issues control instruction to displacement drive unit
(3).
10. system is added in reagent quantitative as claimed in claim 9, which is characterized in that when imaging identification unit detects identification
The gray value of image in area (101) experienced from it is small become larger again from the process to become smaller greatly when, then be determined with a drips.
11. system is added in reagent quantitative as claimed in claim 7, which is characterized in that
Control unit is also used to when drop total quantity is equal to preset value, issues control instruction to displacement drive unit (3) to drive
Dynamic plunger (2) move up a distance along reagent chamber (1) axis.
12. system is added in reagent quantitative as claimed in claim 7, which is characterized in that the determination method of preset value in controller
Are as follows: firstly, production reagent chamber (1), when the plunger (2) in displacement drive unit (3) driving biochip reagent chamber (1) is with speed
When V1 is at the uniform velocity moved down, the volume m for the single dropping liquid drop dripped from reagent chamber (1) drop mouth is calculated；Then, it is added with needs
For destination agent amount divided by m, obtained quotient, which is rounded, obtains drop preset value；Wherein, m is less than or equal to the target examination for needing to be added
Dosage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116196994A (en) * | 2023-05-04 | 2023-06-02 | 合肥机数量子科技有限公司 | Self-adaptive oscillating drip breaking needle head assembly |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4314484A (en) * | 1979-10-09 | 1982-02-09 | University Of Utah Research Foundation | Self-compensating optical drop count apparatus for measuring volumetric fluid flow |
| US4936828A (en) * | 1987-06-02 | 1990-06-26 | Kophu Chiang | Liquid drop image sensor |
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2019
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| US4314484A (en) * | 1979-10-09 | 1982-02-09 | University Of Utah Research Foundation | Self-compensating optical drop count apparatus for measuring volumetric fluid flow |
| US4936828A (en) * | 1987-06-02 | 1990-06-26 | Kophu Chiang | Liquid drop image sensor |
| CN208109479U (en) * | 2018-09-28 | 2018-11-16 | 湖南乐准智芯生物科技有限公司 | A kind of micro quantitative determination sampling system |
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| FERENCHAK P. ET AL.: ""Drop Size and Rate in Parenteral Infusion"", 《SURGERY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116196994A (en) * | 2023-05-04 | 2023-06-02 | 合肥机数量子科技有限公司 | Self-adaptive oscillating drip breaking needle head assembly |
| CN116196994B (en) * | 2023-05-04 | 2023-07-21 | 合肥机数量子科技有限公司 | Self-adaptive oscillating drip breaking needle head assembly |
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| Publication number | Publication date |
|---|---|
| CN109759160B (en) | 2019-07-30 |
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