CN109757307A - Xianggu mushroom strain and its industrial planting method suitable for factory culture - Google Patents
Xianggu mushroom strain and its industrial planting method suitable for factory culture Download PDFInfo
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Abstract
The present invention relates to mushroom factory technical field of cultivation, and in particular to a kind of Xianggu mushroom strain and its industrial planting method suitable for factory culture.The Xianggu mushroom strain suitable for factory culture is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC 16919, entitled " seven rivers 17 " the deposit date is on December 25th, 2018；The industrial planting method of the Xianggu mushroom strain suitable for factory culture, step include: to prepare strain, production bacteria stick or nest mouth bacterium bag, inoculation, preculture, culture, management of producing mushroom.The present invention solves the problems, such as that pollution rate present in lentinus edodes strain stick traditional mode of production is high, yield is unstable, the decline of fruiting quality, the biological transformation ratio of two damp mushrooms is 70%-83%, 98% or more bacteria stick can normally budding fruiting, and fruiting is neat, and mushroom shape is medium-and-large-sized, shape rounding.
Description
Technical field
The present invention relates to mushroom factory technical field of cultivation, and in particular to a kind of Xianggu mushroom strain suitable for factory culture
And its industrial planting method.
Background technique
China is the first big country of Edible Fungi, and China's mushroom total output in 2017 is at 9,000,000 tons or more, mushroom annual output
Increase year by year.Meanwhile with the development of the mushrooms processed goods such as mushroom sauce, new work is added to being surging forward for mushroom industry
Power.However, most China's mushroom main producing region production models fall behind, strain adaptability, strain quality outstanding problem cause repeatly
Occurs the problems such as traditional mushroom plantation batch pollution, spawn degeneration and fruiting quality, inefficiency repeatly.
Strain is the premise of mushroom plant cultivation, directly determines stable yields and high yield, cooperation high efficiency operation mode, dedicated
Automated production equipment and stable planting environment, mushroom factory production increasingly becomes new edible mushroom hot spot.
Summary of the invention
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of Xianggu mushroom strain suitable for factory culture,
Solve the problems, such as that pollution rate present in lentinus edodes strain stick traditional mode of production is high, yield is unstable, the decline of fruiting quality；The present invention also provides
Its industrial planting method.
Xianggu mushroom strain of the present invention suitable for factory culture is preserved in Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center, deposit number is CGMCC 16919, and the deposit date is on December 25th, 2018, entitled " seven rivers
17 ", it is to be selfed by mushroom kind " seven rivers 2 " spore, is obtained through systematic breeding.
Xianggu mushroom strain biological characteristics are as follows:
Bacterium colony: white mycelium, bacterium colony is velvet-like, and protuberance has belt, gradually becomes orange after light-exposed aging；The bacterium colony back side
It is light yellow, no water colo(u)r.
Mycelia microstructure: mycelia wall is smooth, thin-walled to slightly heavy wall, has clamp connection, common separation and branch, wide 1.4-
6.5μm。
Fructification feature: medium-and-large-sized fructification, cap rounding, diameter 55-73mm, cap longitudal section is arc-shaped, cap table
Face covers scale, and scale is small, is mainly distributed on cap edge, and centre is few.Cap thickness 18-26mm, bacterial context is thick, and lamella is milky white
2-3mm or so, stem column is upper coarse and lower fine, and length 3.8-4.8cm has cilium.
Mushroom " seven rivers 17 " method for strain breeding thereof is as follows:
The preferable fructification of fresh growing way for acquiring the cultivation of " seven rivers 2 " bacterial strain, cuts off stem in an aseptic environment, will there is bacterium
Pleat is tipped upside down on one side on plate after 24 hours, collects spore print, is diluted spore with sterile water, is counted with blood counting chamber, will
Spore concentration is diluted to 4 × 105A/ml respectively takes 50 microlitres to be coated on diameter 90cmPDA plate, and 25 DEG C are protected from light culture 10 days,
Occurs bacterium colony on plate, 150 colony edge mycelia of picking are transferred on new PDA plate, and picking bacterium again is protected from light after culture 7 days
It falls the small mycelia block in edge and is transferred to new PDA plate, repeat purifying 3 times, it is oblique that microscopy has the bacterium colony of clamp connection to be transferred to PDA
After cultivating 10 days on face, 4 DEG C are saved backup.Antagonistic experiment is carried out to the cultural hypha object of acquisition, is eliminated and parent's indifference
Cultural hypha object obtains 115 cultural hypha objects, into fruiting screening stage.Cap rounding, bacterium are collected in primary dcreening operation fruiting stage
Handle is short, and fructification best in quality carries out tissue separation, obtains stable double-core mycelia.Secondary screening, comparison experiment are carried out later,
The bacterial strain for filtering out number 17 carries out pilot scale and expands experiment, carries out stability observing, entitled " seven rivers 17 ".
The industrial planting method of Xianggu mushroom strain of the present invention suitable for factory culture, step include: to prepare
Strain, production bacteria stick or nest mouth bacterium bag, inoculation, preculture, culture, management of producing mushroom.
(1) prepare strain:
Using following any method:
Method one:
Strain includes parent species, original seed and production kind.
Parent species: cultural hypha object is placed in the PDA test tube after sterilizing, cultural hypha object is 4-5mm square, at 25 DEG C
Constant temperature incubation, mushroom mycelium use after covering with as parent species.
Original seed: after poplar batten is impregnated in water, dipping cornmeal mush and wheat bran, then pack sealing, and 121 DEG C of high pressure go out
Bacterium 1-3h is aseptically inoculated with parent species after cooling, and culture covers with full bag to mycelia under 25 DEG C of environment, obtains original seed wood
Item.
Production kind: hard leaf wood sawdust is mixed with wheat bran 4:1-5:1 in mass ratio, and adding water to water content is 58-
60wt%, pack sealing, sterilizing 1-3h hours of 121 DEG C of high pressure after cooling, aseptically access 1 original seed batten, 25
Culture covers with full bag to mycelia under DEG C environment, obtains production kind, it is spare to be put into 0~7 DEG C of Cold storage in the refrigerator.
Method two:
Strain includes conical flask liquid spawn and liquid fermentation tank strain.
Conical flask liquid spawn: being cut to 4-5mm square fritter for cultural hypha object, and 4-10 block is taken to be placed in the cone after sterilizing
It in shape bottle fluid nutrient medium, stirs evenly, the constant temperature incubation at 25 DEG C, makes after Lenlinus edodes ball is grown well after 7-8 days as second level kind
With.Portugal is added the preparation method comprises the following steps: every liter of water is added after potato 100-500g is cooked filters in conical flask fluid nutrient medium in filtrate
Grape sugar 10-30g, peptone 1-5g, potassium dihydrogen phosphate 1-4g, anhydrous magnesium sulfate 0.5-2g are uniformly mixed.
Fermentor liquid strain: it will be accessed in fermentor liquid culture medium under liquid spawn sterile working in conical flask, tank
Pressure is maintained at 0.01-0.05MPa, and constant temperature incubation at 25 DEG C uses for 7-9 days when post-fermentation liquid is thick.Fermentor liquid culture medium
Formula is the preparation method comprises the following steps: be added dregs of beans 1-5g, glucose 10-30g, yeast extract 1-5g, potassium dihydrogen phosphate 1-4g, nothing in every liter of water
Water magnesium sulfate 0.5-2g, defoaming agent 0.1-1ml are uniformly mixed.
(2) bacteria stick or nest mouth bacterium bag are produced:
The hard sawdust quality accounting 75-85% such as applewood or toothed oak wood, granularity is in 0.3-1cm, wheat bran quality accounting
15%-25%, gypsum 0-1%, adding water to water content after mixing evenly is 55-59wt%, lime tune pH value is added to 6.5-7,
It is packed using Full-automatic sack filling machine；It is packed into the low-pressure high-density polyethylene bag of 15cm × 55cm, every bag of (stick) diameter after filler
9.5-10cm, high 39-41cm, quality 2.0-2.5kg, i.e. bacteria stick；Or it is packed into 16 × 35mm nest mouth bacterium bag；By bacteria stick or nest
Mouth bacterium bag is packed into high-pressure sterilizing pot, sterilizes 6-10 hours at 120 DEG C, cooling in ten thousand grades of cleaning shops after sterilizing, chilling room
20 DEG C of temperature, placing 24 hours makes bacteria stick be cooled to 30 DEG C or less.
(3) it is inoculated with:
Using full-automatic mushroom solid vaccination machine, the production kind after cleaning is aseptically sloughed into plastic outer bag and is put into
Bacteria stick is put into inoculation device by inoculation device strain hopper one by one, and every stick is inoculated with 4 hole locations, average each production kind inoculation bacteria stick 15-
18 sticks；Or by fermentor liquid Spawn incubation it is good after, pipeline connects fermentor and nest mouth bacterium bag inoculation device, to pipeline and inoculation device
Steam sterilizing 20min is carried out, under aseptic condition, inoculation device is opened and is inoculated with, sponge plug after the bacterium bag that inoculation is completed sterilizes
Sealing is transferred to the culture bacterium germination stage.
(4) preculture:
After inoculation, bacteria stick is put into plastic crate, basket is placed on culturing rack, each culturing rack places 30 baskets, culturing rack
The preculture workshop that purification rank is 100,000 grades is transferred to after piling；23-27 DEG C of set temperature of preculture workshop, humid control
In 30-80%, half-light culture, CO2Concentration is set in 1000-4000ppm；Stationary culture 12-16 days, when bacteria stick mycelia grows
When to diameter 7cm or more, bacteria stick is transferred to normal culturing room.
(5) it cultivates:
23-26 DEG C of culturing room's set temperature, humid control is in 30-80%, half-light culture, CO2Concentration is set in 1500-
5000ppm；When mycelia grows to diameter 12-18cm, first time acanthopore is carried out with row's formula pricker and leads to oxygen, 5- after mycelia is covered with
10 days, bacteria stick started to generate knob protrusion, carries out secondary acanthopore using spot-punching machine and leads to oxygen；Culturing room's setting temperature after secondary acanthopore
23-26 DEG C of degree, humid control is in 40-70%, 100LX or more illumination cultivation, CO2Concentration is set in 1500-5000ppm；Total bacterium
Achievable culture in age 85-90 days, into management of producing mushroom.
(6) management of producing mushroom:
Fruiting greenhouse is to take off the lentinus edodes strain stick for completing culture with refrigeration, humidification and the automation control of ventilation greenhouse
Polybag is removed, is put in fruiting greenhouse layer frame, control temperature is at 12-20 DEG C, humidity 80-90%, CO2Concentration 500-
1500ppm；It opens water spray to humidify bacteria stick to 1.9-2.2kg, buddings after 5-6 days and carry out dredging flower bud, stay flower bud 12-15, after 4 days
It can be harvested.
Temperature is controlled after harvesting at 20-25 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm, illumination 100LX with
On, after recuperation 15-20 days, control temperature at 12-20 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm stimulation is buddingged, and is sprayed
Water humidifies bacteria stick to 1.5-1.8kg, and the second tide harvesting is carried out after fruiting of buddingging.It repeats above operation, can carry out after harvesting
The harvesting of 3-5 tide.
Compared with prior art, the invention has the following beneficial effects:
(1) Xianggu mushroom strain of the invention, bacteria stick pollution rate is lower, is no more than 0.5%, the speed of growth is very fast, and bacteria stick is buddingged
Fastly, under the conditions of 15-18 DEG C of temperature, restocking is buddingged for 3-5 days after sprinkling water, two damp mushroom production cycle average out to 132 days, wherein bacterium germination
Phase 50 days, veraison 37 days, fruiting phase 45 days；
(2) Xianggu mushroom strain of the invention, fructification be medium-and-large-sized, cap rounding, diameter 55-73mm, thickness 18-26mm,
Bacterial context is thick, and milky white 2-3mm of lamella or so, stem column is upper coarse and lower fine, and length 3.8-4.8cm, single mushroom individual difference is small, mushroom
Abnormal rate is below 5%；
(3) industrial planting method of Xianggu mushroom strain of the invention, bacteria stick yield rate reach 95% or more, much higher than similar
80% or so of production；The biological transformation ratio of two damp mushrooms is 75%-83%, and 98% or more bacteria stick can normally budding fruiting, out
Mushroom is neat, and mushroom shape is medium-and-large-sized, shape rounding.
Specific embodiment
The present invention will be further described with reference to embodiments, but protection scope of the present invention is not limited only to this, the neck
Domain professional changes to made by technical solution of the present invention, is within the scope of protection of the invention interior.
Embodiment 1
It uses bacterial strain of the present invention " seven rivers 17 " and parent strain " seven rivers 2 " for test material, uses PDA culture medium.Point
Not by opposite culture in above two strain inoculated to culture dish (7 groups), constant temperature incubation at 25 DEG C is observed in culture dish after 20 days
Two bacterial strain bacterium colonies, the antagonism situation in each culture medium are as shown in table 1, wherein " +++ " indicates antagonism clearly, and " ++ " indicates
Antagonism is more apparent, and "+" expression seemingly has antagonism.
Antagonism situation in each culture dish of table 1
| Serial number | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Antagonistic results | +++ | +++ | +++ | +++ | +++ | ++ | +++ |
As it can be seen from table 1 being complete with " seven rivers 2 " " seven rivers 17 " bacterial strain that be starting strain obtain through spore hybridization
Different mushroom new varieties.
Embodiment 2
(1) prepare strain:
Conical flask liquid spawn: being cut to 4-5mm square fritter for cultural hypha object, and 4-10 block is taken to be placed in the cone after sterilizing
It in shape bottle fluid nutrient medium, stirs evenly, the constant temperature incubation at 25 DEG C, makes after Lenlinus edodes ball is grown well after 7-8 days as second level kind
With.Portugal is added the preparation method comprises the following steps: every liter of water is added after potato 100-500g is cooked filters in conical flask fluid nutrient medium in filtrate
Grape sugar 10-30g, peptone 1-5g, potassium dihydrogen phosphate 1-4g, anhydrous magnesium sulfate 0.5-2g are uniformly mixed.
Fermentor liquid strain: it will be accessed in fermentor liquid culture medium under liquid spawn sterile working in conical flask, tank
Pressure is maintained at 0.01-0.05MPa, and constant temperature incubation at 25 DEG C uses for 7-9 days when post-fermentation liquid is thick.Fermentor liquid culture medium
Formula is the preparation method comprises the following steps: be added dregs of beans 1-5g, glucose 10-30g, yeast extract 1-5g, potassium dihydrogen phosphate 1-4g, nothing in every liter of water
Water magnesium sulfate 0.5-2g, defoaming agent 0.1-1ml are uniformly mixed.
(2) nest mouth bacterium bag is produced:
Select granularity in 0.3-1cm toothed oak wood sawdust 79wt%, wheat bran 20wt%, gypsum 1wt%, after mixing evenly plus water
To water content 59wt%, appropriate lime tune pH to 6.5-7 is added, is packed into 16 × 35mm nest mouth bacterium bag, 120 DEG C of autoclave sterilizations
5 hours, cooling was spare.
(3) it is inoculated with:
After fermentor liquid Spawn incubation is good, pipeline connects fermentor and nest mouth bacterium bag inoculation device, to pipeline and inoculation device
Carry out steam sterilizing 20min.Under aseptic condition, opens inoculation device and be inoculated with, sponge plug after the bacterium bag sterilizing that inoculation is completed
Sealing is transferred to the culture bacterium germination stage.
(4) preculture:
After inoculation, bacteria stick is put into 49cm × 38cm × 11cm plastic crate, every basket of 9 sticks of placement place basket after piling
On culturing rack, each culturing rack places 30 baskets, and culturing rack is transferred to the preculture vehicle that purification rank is 100,000 grades after piling
Between.25 DEG C of preculture workshop set temperature, humid control is 80%, half-light culture, CO2Concentration is set in 1000ppm.Work as bacteria stick
When mycelia grows to diameter 7cm, bacteria stick is transferred to normal culturing room.
(5) it cultivates
25 DEG C of culturing room's set temperature, humid control is 50%, half-light culture, CO2Concentration is set in 3500ppm.Work as bacterium
Silk is when growing to diameter 12cm, carries out first time acanthopore with row's formula pricker and leads to oxygen, mycelia cover with after 5 days, bacteria stick starts generation tumor
Shape object protrusion, carries out secondary acanthopore using spot-punching machine and leads to oxygen.26 DEG C of culturing room's set temperature after secondary acanthopore, humid control exists
70%, 100LX intensity of illumination culture, CO2Concentration is set in 4500ppm.Total cell age achievable culture in 85 days, into fruiting pipe
The reason stage.
(6) management of producing mushroom:
The mature lentinus edodes strain stick for completing culture is sloughed into polybag, is put in fruiting greenhouse layer frame, opens spray for bacterium
Stick is humidified to 2.2kg, control day and night 18-20 DEG C of temperature difference daytime, and 10-12 DEG C of night, humidity 80-90%, ventilation keeps CO in time2
Concentration 500-1000ppm.Budding after 5-6 days and carry out dredging flower bud, stay flower bud 12-15, after 4 days mushroom individual it is long to 5cm or more can be into
Row harvesting.
Temperature is controlled after harvesting at 20-25 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm, illumination 100LX with
On, after recuperation 15-20 days, control temperature at 12-20 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm stimulation is buddingged, and is sprayed
Water humidifies bacteria stick to 1.5-1.8kg, and the second tide harvesting is carried out after fruiting of buddingging.It repeats above operation, can carry out after harvesting
The harvesting of 3-5 tide.
In process of production, 99.5% bacteria stick is normally buddingged fruiting, and fruiting is neat, and mushroom shape is medium-and-large-sized, shape rounding, single
Mushroom individual difference is small, mushroom abnormal rate 2.8%.It randomly selects 100 mushrooms to measure, cap average diameter 70.86mm, put down
Equal thickness 24.32mm, stem average length 4.3cm.Two damp mushroom production cycles were 132 days, wherein bacteria developing period 50 days, veraison 37
It, fruiting phase 45 days, the biological transformation ratio of two damp mushrooms was 78.2%, and the biological transformation ratio of 5 damp mushrooms is 102.5%.
Embodiment 3
(1) strain prepares:
Parent species: cutting cultural hypha object is 4mm × 4mm size, and cultural hypha object is placed in the PDA test tube after sterilizing,
The constant temperature incubation at 25 DEG C, mushroom mycelium use after covering with as parent species.
Original seed: after the poplar batten of 0.7cm × 0.7cm thickness, length 15cm are impregnated in water, cornmeal mush and bran are dipped
Skin is fitted into polyethylene or polypropylene plastics pocket, per it is packed enter 40-70 branch, 121 DEG C of high pressure sterilize 2 hours, after cooling, in nothing
Parent species are inoculated under the conditions of bacterium, culture covers with full bag to mycelia under 25 DEG C of environment, obtains original seed batten.
Production kind: 3-6mm sieving hard leaf wood sawdust is mixed with wheat bran 4:1 in mass ratio, water is added to adjust water content
To 58-60%, it is fitted into 17 × 35cm polypropylene plastics pocket and seals 121 DEG C of sterilizings 2 hours, sterile access 1 after bacterium bag is cooling
Original seed batten, culture to mycelia covers with full bag under 25 DEG C of environment, and it is spare can be put into 0~7 DEG C of Cold storage in the refrigerator for production kind after purseful,
Dichloro isocyanuric sodium urate disinfection water sterilization production kind surface is used before inoculation.
(2) bacteria stick is produced:
By 80wt% applewood sawdust (particle 0.3-0.8cm), 19wt% wheat bran and the mixing of 1wt% gypsum are stirred evenly
After add water to water content 57wt%, add lime tune pH value be 6.8, packed using Full-automatic sack filling machine, polybag 15cm
× 55cm low-pressure high-density polyethylene bag, every bag of quality 2.2kg after filler.Bacteria stick is packed into high-pressure sterilizing pot, at 120 DEG C
Sterilizing 10 hours, cooling in ten thousand grades of cleaning shops after sterilizing, chilling room temperature is 20 DEG C, and placing 24 hours makes bacteria stick cool down
To 30 DEG C or less.
(3) it is inoculated with:
Using full-automatic mushroom solid vaccination machine, the production kind after cleaning is aseptically sloughed into plastic outer bag and is put into
Bacteria stick is put into inoculation device by inoculation device strain hopper one by one, and every stick is inoculated with 4 hole locations, average each production kind inoculation bacteria stick 15-
18 sticks.
(4) preculture:
After inoculation, bacteria stick is put into 49cm × 38cm × 11cm plastic crate, every basket of 9 sticks of placement place basket after piling
On culturing rack, each culturing rack places 30 baskets, and culturing rack is transferred to the preculture vehicle that purification rank is 100,000 grades after piling
Between.25 DEG C of preculture workshop set temperature, humid control is 80%, half-light culture, CO2Concentration is set in 1000ppm.Work as bacteria stick
When mycelia grows to diameter 7cm, bacteria stick is transferred to normal culturing room.
(5) it cultivates
25 DEG C of culturing room's set temperature, humid control is 50%, half-light culture, CO2Concentration is set in 3500ppm.Work as bacterium
Silk is when growing to diameter 12cm, carries out first time acanthopore with row's formula pricker and leads to oxygen, mycelia cover with after 5 days, bacteria stick starts generation tumor
Shape object protrusion, carries out secondary acanthopore using spot-punching machine and leads to oxygen.26 DEG C of culturing room's set temperature after secondary acanthopore, humid control exists
70%, 100LX intensity of illumination culture, CO2Concentration is set in 4500ppm.Total cell age achievable culture in 85 days, into fruiting pipe
The reason stage.
(6) management of producing mushroom:
The mature lentinus edodes strain stick for completing culture is sloughed into polybag, is put in fruiting greenhouse layer frame, opens spray for bacterium
Stick is humidified to 2.2kg, control day and night 18-20 DEG C of temperature difference daytime, and 10-12 DEG C of night, humidity 80-90%, ventilation keeps CO in time2
Concentration 500-1000ppm.Budding after 5-6 days and carry out dredging flower bud, stay flower bud 12-15, after 4 days mushroom individual it is long to 5cm or more can be into
Row harvesting.
Temperature is controlled after harvesting at 20-25 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm, illumination 100LX with
On, after recuperation 15-20 days, control temperature at 12-20 DEG C, humidity 80-90%, CO2Concentration 500-1500ppm stimulation is buddingged, and is sprayed
Water humidifies bacteria stick to 1.5-1.8kg, and the second tide harvesting is carried out after fruiting of buddingging.It repeats above operation, can carry out after harvesting
The harvesting of 3-5 tide.
In process of production, 99% bacteria stick is normally buddingged fruiting, and fruiting is neat, and mushroom shape is medium-and-large-sized, shape rounding, single mushroom
Individual difference is small, mushroom abnormal rate 3.5%.It randomly selects 100 mushrooms to measure, cap average diameter 70.43mm, it is average
Thickness 23.52mm, stem average length 4.2cm.Two damp mushroom production cycles were 132 days, wherein bacteria developing period 50 days, veraison 37
It, fruiting phase 45 days, the biological transformation ratio of two damp mushrooms was 75.7%, and the biological transformation ratio of 5 damp mushrooms is 101.4%.
Comparative example 1
Using mushroom " seven rivers 2 " bacterial strain, factory culture is equally used, method is same as Example 2.
In process of production, 98% bacteria stick is normally buddingged fruiting, mushroom abnormal rate 12.3%.Randomly select 100 perfume (or spice)
Mushroom measures, cap average diameter 62.43mm, average thickness 20.35mm, stem average length 3.5cm.Two damp mushrooms production week
Phase is 132 days, wherein bacteria developing period 50 days, and veraison 37 days, fruiting phase 45 days, the biological transformation ratio of two damp mushrooms was 78.2%, 5
The biological transformation ratio of damp mushroom is 92.7%.
Comparative example 2
Using mushroom " 0912 " bacterial strain, factory culture is equally used, method is same as Example 3.
In process of production, 96% bacteria stick is normally buddingged fruiting, and mushroom shape is smaller, mushroom abnormal rate 14.9%.It is random to take out
100 mushrooms are taken to measure, cap average diameter 57.98mm, average thickness 18.37mm, stem average length 2.8cm.Two
The damp mushroom production cycle is 141 days, wherein bacteria developing period 49 days, and veraison 40 days, fruiting phase 52 days, the biological transformation ratio of two damp mushrooms was
74.7%, the biological transformation ratio of 5 damp mushrooms is 89.4%.
Claims (10)
1. a kind of Xianggu mushroom strain suitable for factory culture, it is characterised in that: be preserved in Chinese microorganism strain preservation management
Committee's common micro-organisms center, deposit number are CGMCC 16919, and the deposit date is on December 25th, 2018.
2. the Xianggu mushroom strain according to claim 1 suitable for factory culture, it is characterised in that: the bacterium colony of Xianggu mushroom strain
Biological property are as follows: white mycelium, bacterium colony is velvet-like, and protuberance has belt, gradually becomes orange after light-exposed aging；The bacterium colony back side
It is light yellow, no water colo(u)r.
3. the Xianggu mushroom strain according to claim 1 suitable for factory culture, it is characterised in that: the mycelia of Xianggu mushroom strain
Microstructure are as follows: mycelia wall is smooth, thin-walled to slightly heavy wall, has clamp connection, common separation and branch, 1.4-6.5 μm wide.
4. the Xianggu mushroom strain according to claim 1 suitable for factory culture, it is characterised in that: the son of Xianggu mushroom strain is real
Body characteristics are as follows: medium-and-large-sized fructification, cap rounding, diameter 55-73mm, cap longitudal section is arc-shaped, and cap surface covers scale,
Scale is small, is mainly distributed on cap edge, and centre is few；Cap thickness 18-26mm, bacterial context is thick, milky white 2-3mm of lamella or so, bacterium
Handle column, upper coarse and lower fine, length 3.8-4.8cm has cilium.
5. a kind of industrial planting method of the described in any item Xianggu mushroom strains suitable for factory culture of claim 1-4,
It is characterized by: step includes: to prepare strain, production bacteria stick or nest mouth bacterium bag, inoculation, preculture, culture, management of producing mushroom；
Prepare strain and use any one following method:
Method one:
Strain includes parent species, original seed and production kind；
Parent species: cultural hypha object is placed in the PDA test tube after sterilizing, constant temperature incubation to mushroom mycelium covers with examination at 25 DEG C
Pipe, obtains parent species；
Original seed: after poplar batten is impregnated in water, dipping cornmeal mush and wheat bran, then pack sealing, 121 DEG C of sterilizing 1- of high pressure
3h is aseptically inoculated with parent species after cooling, and culture covers with full bag to mycelia under 25 DEG C of environment, obtains original seed batten；
Production kind: hard leaf wood sawdust is mixed with wheat bran 4:1-5:1 in mass ratio, and adding water to water content is 58-60wt%,
Pack sealing, sterilizing 1-3h hours of 121 DEG C of high pressure after cooling, aseptically access 1 original seed batten, in 25 DEG C of environment
Lower culture covers with full bag to mycelia, obtains production kind, it is spare to be put into 0~7 DEG C of Cold storage in the refrigerator；
Method two:
Strain includes conical flask liquid spawn and liquid fermentation tank strain；
Conical flask liquid spawn: cultural hypha object is placed in the conical flask fluid nutrient medium after sterilizing, constant temperature incubation at 25 DEG C
7-8 days；
Fermentor liquid strain: liquid spawn in conical flask is accessed into liquid fermentation tank culture medium, tank pressure is maintained at 0.01-
0.05MPa, constant temperature incubation 7-9 days at 25 DEG C.
6. the industrial planting method of the Xianggu mushroom strain according to claim 5 suitable for factory culture, feature exist
In: the step of producing bacteria stick or nest mouth bacterium bag are as follows: by 75-85wt% hard sawdust, 15-25wt% wheat bran, 0-1wt% gypsum is mixed
It closes, adding water to water content after mixing evenly is 55-59wt%, and adding lime tune pH value is 6.5-7, and pack sealing obtains bacterium
Stick or nest mouth bacterium bag sterilize 6-10 hours at 120 DEG C, are cooled to 30 DEG C or less.
7. the industrial planting method of the Xianggu mushroom strain according to claim 5 suitable for factory culture, feature exist
In: inoculation step are as follows: production kind is inoculated into bacteria stick using full-automatic mushroom solid vaccination machine, or is connect using nest mouth bacterium bag
Fermentor liquid strain is inoculated into nest mouth bacterium bag by kind machine.
8. the industrial planting method of the Xianggu mushroom strain according to claim 5 suitable for factory culture, feature exist
In pre-culture step are as follows: by after inoculation bacteria stick or nest mouth bacterium bag be transferred to preculture workshop, at 23-27 DEG C of temperature, humidity 30-
Under the conditions of 80%, half-light culture, CO2Concentration is set in 1000-4000ppm；Stationary culture 12-16 days, when bacteria stick mycelia grows
When to diameter 7cm or more, bacteria stick or nest mouth bacterium bag are transferred to training workshop.
9. the industrial planting method of the Xianggu mushroom strain according to claim 5 suitable for factory culture, feature exist
In incubation step are as follows: by after preculture bacteria stick or nest mouth bacterium bag under the conditions of 23-26 DEG C of temperature, humidity 30-80%, continue
Half-light culture, CO2Concentration is set in 1500-5000ppm；When mycelia grows to diameter 12-18cm, it is logical to carry out first time acanthopore
Oxygen carries out secondary acanthopore and leads to oxygen 5-10 days after mycelia is covered with；After secondary acanthopore leads to oxygen, at 23-26 DEG C of temperature, humidity 40-
Under the conditions of 70%, illumination cultivation, CO2Concentration is set in 1500-5000ppm；Total cell age completion in 85-90 days culture, into fruiting
Management.
10. the industrial planting method of the Xianggu mushroom strain according to claim 5 suitable for factory culture, feature exist
In management of producing mushroom step are as follows: the lentinus edodes strain stick or nest mouth bacterium bag that will complete culture take off bag, controlled at 12-20 DEG C, humidity
80-90%, CO2Lentinus edodes strain stick is humidified, is buddingged after 5-6 days by concentration 500-1500ppm, water spray, is carried out dredging flower bud, is stayed flower bud 12-15
It is a, it can be harvested after 4-6 days；20-25 DEG C of temperature is controlled after harvesting, humidity 80-90%, CO2Concentration 500-1500ppm, light
According to recuperation 15-20 days；Then 12-20 DEG C of temperature is controlled, humidity 80-90%, CO2Concentration 500-1500ppm, stimulation are buddingged, spray
Water humidifies lentinus edodes strain stick, and the second tide harvesting is carried out after fruiting of buddingging；It is repeated above operation after harvesting, carries out the harvesting of 3-5 tide.
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| CN113337408A (en) * | 2021-07-20 | 2021-09-03 | 河北省科学院生物研究所 | Lentinus edodes strain JXB5 and application thereof |
| CN113337408B (en) * | 2021-07-20 | 2022-03-29 | 河北省科学院生物研究所 | Lentinus edodes strain JXB5 and application thereof |
| CN114342736A (en) * | 2022-01-06 | 2022-04-15 | 湖北昌利菌业开发有限公司 | Standardized production process of mushroom sticks |
| CN114303794A (en) * | 2022-02-21 | 2022-04-12 | 山东七河生物科技股份有限公司 | Shiitake culture medium and shiitake cultivation method |
| CN116058230A (en) * | 2022-11-07 | 2023-05-05 | 丽水市农林科学研究院 | Lentinus edodes strain L808-Ndt and application thereof |
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