CN109749950A

CN109749950A - A kind of agrobacterium rhizogenes microbial inoculum and preparation method thereof for promoting long bean to take root

Info

Publication number: CN109749950A
Application number: CN201711059049.4A
Authority: CN
Inventors: 徐洪伟; 王其传
Original assignee: HUAI'AN CHAIMIHE AGRICULTURAL TECHNOLOGY DEVELOPMENT Co Ltd; Jilin Normal University
Current assignee: HUAI'AN CHAIMIHE AGRICULTURAL TECHNOLOGY DEVELOPMENT Co Ltd; Jilin Normal University
Priority date: 2017-11-01
Filing date: 2017-11-01
Publication date: 2019-05-14

Abstract

The present invention relates to a kind of agrobacterium rhizogenes (15834) microbial inoculums and preparation method thereof that promotion long bean is taken root, belong to biological organic fertilizer research and development field, agrobacterium rhizogenes has the function of promoting plant establishment growth, there is important influence to plant growth and development, therefore, use OD value for the agrobacterium rhizogenes bacterium solution of 0.4-0.6, 10 parts of yeast powder, 6 parts of NaCl, 10 parts of mixings of tryptone are configured to microbial inoculum, impose on the long bean seedling root in tri-leaf period, fertilising in every 15 days is primary, it is applied fertilizer 3 times altogether until bearing pods, since agrobacterium rhizogenes and long bean form a kind of symbiosis, it have stimulated the branch of long bean root and root growth and increase root absorption area, promote its growth and development, it ensure that survival rate of plant, it is low to overcome long bean fruit quality in production, the problem of low output, in agricultural On can be used for improving the quality of crops and increase its yield, have huge economic benefit.

Description

A kind of agrobacterium rhizogenes microbial inoculum and preparation method thereof for promoting long bean to take root

Technical field

The present invention relates to a kind of preparation methods of agrobacterium rhizogenes microbial inoculum that promotion long bean is taken root, and belong to biological organic fertilizer and grind Send out technical field.

Background technique

Long bean is the annual vegetable of Rosales pulse family Vigna, long bean it is adaptable, both can be with outdoor cropping, can also With Greenhouse, while can be with whole year production, four seasons listing, it has also become most popular one of vegetable crop on dining table. But due to planting year after year, soil starts that large area is hardened, and the soil-borne diseases such as continuous cropping, root-knot nematode are got worse, and causes root system not Phenomena such as sound, main side root branch lacks, especially fibrous root is undeveloped, only transporting root, and color is black dull, or even death of rotting, white Color extends root and capillary root is few, therefore results in the reduction of long bean quality.Agrobacterium rhizogenes can promote hair produced by plant Root consolidates root system of plant, promotes plant growth and development, therefore by preparation agrobacterium rhizogenes microbial inoculum, imposes on crop, promote its root It is growth and development, has great significance and potential economic benefit to crop quality is improved.

Summary of the invention

For above-mentioned phenomenon, it is an object of the invention to utilize the distinctive work for promoting plant root growth of agrobacterium rhizogenes With solution long bean due to root system undeveloped caused the problems such as survival rate is low, fruit quality is low, provides one during the growth process The preparation method for the agrobacterium rhizogenes microbial inoculum that kind promotes long bean to take root.

The present invention is realized by following technical scheme:

1) agrobacterium rhizogenes microbial inoculum component and content are as follows:

It is 0.4-0.6 by the activation of agrobacterium rhizogenes strain 15834 to OD value, the bacterium solution 50ml after taking activation is separately added into 10 parts of yeast powder, 10 parts of tryptone, is diluted with water to 200 times at 6 parts of NaCl, mixes, i.e. acquisition agrobacterium rhizogenes microbial inoculum.

2) application that agrobacterium rhizogenes microbial inoculum promotes long bean to take root

In buying common long bean seed on the market, field is sowed, until long bean seedling tri-leaf period, the root of hair agriculture bar that will be prepared Bacteria agent is applied to long bean seedling root, every plant of about 50ml dilution, and fertilising in every 15 days is once until long bean bears pods, long bean plant There is apparent well developed root system in developmental process, grow vigorous, the high feature of setting percentage, substantially increase crop quality and produce Amount.

The present invention has the effect of following positive:

1, a kind of preparation method for the agrobacterium rhizogenes microbial inoculum for promoting long bean to take root of the present invention, using agrobacterium rhizogenes strain 15834, agrobacterium rhizogenes can generate symbiosis with crop root, keep its indefinite root growth rapid, by consolidating root system of plant, Achieve the effect that improve crop quality.

2, a kind of preparation method for the agrobacterium rhizogenes microbial inoculum for promoting long bean to take root of the present invention, using agrobacterium rhizogenes bacterium Liquid, the components such as yeast powder, NaCl, tryptone have that cost is relatively low, easily obtain, the simple advantage of preparation method is easy to throw Enter production.

3, it using a kind of preparation method of agrobacterium rhizogenes microbial inoculum that promotion long bean is taken root of the invention, solves long bean and exists Due to root system undeveloped caused the problem of survival rate is low, fruit quality is low, low output in growth course, there is huge economy Benefit.

Detailed description of the invention

Fig. 1 is the long bean condition of rooting figure for growing 30 days, and a left side is, using the long bean of agrobacterium rhizogenes microbial inoculum, the right side is control Group.

Fig. 2 is that 80d long bean grows up to situation map, and a left side is, using the long bean of agrobacterium rhizogenes microbial inoculum, the right side is control group.

Specific embodiment

Below with reference to case study on implementation, the invention will be further described:

Case study on implementation

It is 0.4-0.6 by the activation of agrobacterium rhizogenes strain 15834 to OD value, the bacterium solution 50ml after taking activation is separately added into Yeast powder 10g, NaCl 6g, tryptone 10g, 200 times are diluted with water to, mixed, is i.e. acquisition agrobacterium rhizogenes microbial inoculum.

In buying common long bean seed on the market, field is sowed, each 30 plants of control group, experimental group, control group does not apply hair Root Agrobacterium microbial inoculum, experimental group apply agrobacterium rhizogenes microbial inoculum, apply fertilizer since long bean seedling tri-leaf period, be applied to long bean seedling Root, every plant of about 50ml microbial inoculum, fertilising in every 15 days is once until long bean bears pods, from long bean tri-leaf period start recording plant strain growth shape Condition, upgrowth situation statistics such as table 1:

1 tri-leaf period of table starts different number of days long bean growing state

As shown in attached drawing 1,2: the result shows that, the long bean for being applied with agrobacterium rhizogenes microbial inoculum has obviously in developmental process High survival rate, well developed root system, grow it is vigorous, the high feature of Pod Bearing Percentage, the plant for less applying agrobacterium rhizogenes microbial inoculum is compared, beans Angle quality and yield increase, result explanation, and a kind of agrobacterium rhizogenes microbial inoculum for promoting long bean to take root of the present invention improves The quality of long bean, to the plantation of agricultural product has important positive effect.

Claims

1. a kind of agrobacterium rhizogenes microbial inoculum for promoting long bean to take root, which is characterized in that the bacteria agent is by following component system It is standby:

。

2. a kind of agrobacterium rhizogenes microbial inoculum for promoting long bean to take root according to claim 1, which is characterized in that the hair Root Agrobacterium bacterium solution is the agrobacterium rhizogenes 15834 that OD value is 0.4-0.6, every doses 50ml.

3. a kind of preparation method for the agrobacterium rhizogenes microbial inoculum for promoting long bean to take root, which is characterized in that this method includes following step It is rapid: it is the bacterium solution after 0.4-0.6 must be activated by the activation of agrobacterium rhizogenes strain 15834 to OD value, the bacterium solution 50ml after taking activation, It is separately added into yeast 10g, NaCl 6g, tryptone 10g, is diluted with water to 200 times, mix, i.e. acquisition agrobacterium rhizogenes bacterium Agent；The agrobacterium rhizogene strain is 15834 bacterial strains, derives from Chinese microorganism strain collection；Yeast powder, NaCl, Tryptone is purchased from inthe Shanmen area, Siping, Jilin province city, sky day hardware, electrical and chemical equipment agency, Tiedong District.

4. a kind of preparation method of agrobacterium rhizogenes microbial inoculum for promoting long bean to take root according to claim 3, feature exist In effective viable bacteria >=2 × 10 of agrobacterium rhizogenes microbial inoculum obtained⁸cuf/g。