CN109735475B - Acid-resistant acetoin-producing bacillus amyloliquefaciens and application thereof - Google Patents
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Abstract
The invention discloses an acid-resistant acetoin-producing Bacillus amyloliquefaciens, which is classified and named as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), has a strain number of NRCB005, is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, has a preservation number of CGMCC NO.17214, and has a preservation date of 2019, 1 month and 18 days. The invention also discloses application of the bacillus amyloliquefaciens. Pure culture tests, laboratory plate tests and greenhouse pot experiments prove that the bacillus amyloliquefaciens strain NRCB005 disclosed by the invention can generate a volatile organic gas acetoin, can remarkably promote the growth of tomatoes in soil with the pH value of 7.0-4.2, can effectively inhibit fusarium oxysporum pathogenic bacteria, corn northern leaf blight pathogenic bacteria and melon blight pathogenic bacteria, and further promotes the growth of the tomatoes, and has a good application prospect.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to bacillus amyloliquefaciens and application thereof in promoting crop growth and preventing plant diseases.
Background
At present, in agricultural production, because agricultural input products such as chemical fertilizers and the like are unreasonably used for a long time, the problems of farmland soil acidification, hardening, secondary salinization and the like are caused, the microbial community structure of the soil rhizosphere is also destroyed, and a plurality of beneficial microorganisms are changed from the dominant flora to the secondary flora. The soil acidification of China has the characteristics of large area, wide distribution, high acidification degree, great harm and the like, wherein the total area of the acidified soil is as high as 2.04 multiplied by 10 8 hm 2 Approximately 22.7% of the national soil area (201 on the first day)4). In recent decades, due to the influence of high-intensity human activities, the process of soil acidification is greatly accelerated, the damage to the ecological environment and agricultural production is aggravated, and the condition is particularly serious in tropical and subtropical regions. Therefore, effective measures are taken to slow down the soil acidification process and improve and repair seriously acidified soil, and the method has important significance for protecting the ecological environment and guaranteeing the sustainable development of agriculture (Xu Renkou, 2015).
The tomato is one of the crops with the largest protected vegetable cultivation area in China, has unique flavor and rich nutrition, and is popular with consumers. However, in order to increase the yield of tomatoes, excessive fertilizer is applied to the tomatoes in the fertilizer, which causes the problems of low quality of tomato fruits, soil hardening and acidification of farmland soil and the like, so how to increase the acid resistance of commercial crops such as tomatoes is not only an important method for improving and utilizing acidified land, but also a fundamental way for increasing the yield by expanding the planting range of crops such as tomatoes.
Plant growth-promoting rhizobacteria, which are bacteria that colonize plant roots and can promote plant growth or inhibit plant diseases, are called plant growth-promoting rhizobacteria (PGPR), and a large number of PRPG strains have been isolated from the rhizosphere of various crops such as rice, wheat, corn, peanut, and the like, and are widely used in the fields of microbial fertilizers and microbial agents. The emergence of plant growth-promoting rhizobacteria provides a new idea for green production increase of tomatoes. Among them, bacillus amyloliquefaciens is an excellent growth-promoting bacterium which not only promotes the growth of plants but also effectively prevents the occurrence of plant diseases, for example, it inhibits the growth of plant pathogenic bacteria, fungi, viruses, nematodes by producing active substances such as antibiotics, antimicrobial proteins or polypeptides; the plant growth is promoted by dissolving phosphorus, dissolving potassium, secreting indoleacetic acid (IAA), gibberellins (GAs) and the like. However, there is little mention of strains that produce volatile compounds that promote crop growth and inhibit the growth of phytopathogens, which can function without contacting the root system. If a strain which has the growth promoting characteristics of general bacillus and can produce a certain amount of volatile substances can be obtained, the range from the microbial fertilizer to the root system when the microbial fertilizer and the microbial agent are applied can be effectively enlarged, and the method has the advantages of high efficiency, safety and convenient application.
Patent CN200810088388.X discloses a composite biocontrol microbial inoculum AR156 containing bacillus and serratia, which can prevent and control various vegetable soil-borne diseases and has the growth promoting effect. Patent CN 201010518034.1 discloses a Bacillus amyloliquefaciens Lx-11 strain with the preservation number of CGMCC No.3789, which has good control effect on rice bacterial leaf streak. Patent CN200910058487.8 discloses a trichoderma preparation for controlling soil-borne diseases of crops, and can significantly increase the yield of tobacco, but none of the above patents mention the growth promoting effect of volatile substances secreted by strains on plants, nor the growth promoting effect of tomato and other crops in acid soil.
Disclosure of Invention
The invention aims to solve the technical problem of providing acid-resistant acetoin-producing bacillus amyloliquefaciens which has excellent capacity of promoting the growth of plants such as tomatoes and the like in acidified soil and can promote the growth of the tomatoes in strong acid soil.
The invention also aims to solve the technical problem of providing the application of the acid-resistant acetoin-producing bacillus amyloliquefaciens.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an acid-resistant acetoin-producing Bacillus amyloliquefaciens is classified and named as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the strain number is NRCB005, the Bacillus amyloliquefaciens is preserved in the China general microbiological culture Collection center, the preservation number is CGMCC NO.17214, and the preservation date is 2019, 1 month and 18 days. The preservation address is microorganism research institute of China academy of sciences, no.3, xilu No.1, beijing, chaoyang, and the area of Tokyo, and the postal code is 100101.
The Bacillus amyloliquefaciens NRCB005 is obtained by separating rhizosphere soil of large paved paddy rice in Yixing city of Jiangsu province in 8 months in 2018 by the inventor, has higher homology with the Bacillus amyloliquefaciens after BLAST comparison in NCBI through 16SrDNA sequence analysis, has 100 percent of similarity, and preliminarily identifies the strain NRCB005 as the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) according to the appearance and physiological and biochemical characteristics. The bacillus amyloliquefaciens NRCB005 is an acetoin secreting strain, the bacillus amyloliquefaciens NRCB005 is an acid-resistant bacillus, the acid resistance of the bacillus amyloliquefaciens NRCB is 7-pH 3.5, bacterial colonies of the bacillus amyloliquefaciens NRCB005 are round, opaque, semitransparent, light yellow, white, convex, irregular in edge and easy to pick up. The bacterium can produce indoleacetic acid, dissolve inorganic phosphorus, and dissolve insoluble potassium, which is the same as most bacilli. In addition, the strain can also generate volatile gas acetoin, plays a role when the strain is not in contact with the root system of crops, effectively enlarges the range from the root system when the microbial fertilizer and the microbial agent are applied, and has the advantages of high efficiency, safety and convenient application. Other characteristics of the strain of the present invention will be described in detail in the following detailed description.
Wherein, the bacillus amyloliquefaciens has better acid resistance and can normally grow in a liquid culture medium in an environment with pH value of 7-pH value of 3.5.
Wherein the Bacillus amyloliquefaciens normally grows in acidified soil at pH 7.0-pH 4.2, preferably at pH 6.0-pH 4.2.
The application of the acid-resistant acetoin-producing bacillus amyloliquefaciens in the production of acetoin by fermentation is also within the protection scope of the invention.
The application of the acid-resistant acetoin-producing bacillus amyloliquefaciens in the acidification of soil to the promotion of plant growth is also within the protection scope of the invention.
Wherein, the plant is preferably tomato.
Wherein the acidified soil is soil with pH value of 7.0-4.2 (preferably pH value of 6.0-4.2).
The application of the acid-resistant acetoin-producing bacillus amyloliquefaciens in inhibiting fusarium oxysporum, protoplasm zea mays or melon fusarium wilt pathogenic bacteria is also within the protection range of the invention.
Has the advantages that: the bacillus amyloliquefaciens NRCB005 provided by the invention can obviously improve the acid resistance of crops such as tomatoes and the like, and the fresh weight of tomatoes inoculated with the bacillus amyloliquefaciens NRCB005 is respectively increased by 52% and 45% in un-acidified soil and acidified soil. Pure culture tests, laboratory plate tests and greenhouse pot experiments prove that the bacillus amyloliquefaciens strain NRCB005 disclosed by the invention can generate volatile organic gas acetoin, can effectively inhibit fusarium oxysporum pathogenic bacteria, corn northern leaf blight pathogenic bacteria and melon blight pathogenic bacteria, and further promotes the growth of tomatoes, and has a good application prospect.
Drawings
FIG. 1 shows the colony morphology of Bacillus amyloliquefaciens NRCB005 on nitrogen fixation medium.
FIG. 2 shows the chromatographic detection result of NRCB005 acetoin, wherein A is 3g/L acetoin standard sample, B is 1/30 fermentation liquid with acidity of pH7 for 48 h.
FIG. 3 is the antagonistic action of Bacillus amyloliquefaciens NRCB005 on Fusarium oxysporum pathogen in example 4
FIG. 4 is the antagonistic effect of Bacillus amyloliquefaciens NRCB005 on Proteus zeae in example 4.
FIG. 5 is the antagonistic action of Bacillus amyloliquefaciens NRCB005 on melon Fusarium wilt pathogen in example 4.
FIG. 6 is a graph showing the growth promoting effect of Bacillus amyloliquefaciens NRCB005 in the pot cultivation in the acidified soil in example 11.
FIG. 7 is a graph showing the pot growth promoting effect of Bacillus amyloliquefaciens NRCB005 in non-acidified soil in example 11.
FIG. 8 shows acid tolerance of the Bacillus amyloliquefaciens strain NRCB005 in example 3.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to the following embodiments. The examples are only intended to illustrate the technical solution of the invention and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that the foregoing embodiments may be modified or partially replaced; such modifications and substitutions do not depart from the scope of the invention as claimed.
EXAMPLE 1 isolation and screening of Strain NRCB005
Placing 10g of rice rhizosphere soil into a conical flask filled with 90mL of salt solution, oscillating for 1h at 28 ℃ under the condition of 200r/min, and preparing into 10g of rice rhizosphere soil -1 10, taking -1 10mL of the soil suspension is put into a bottle filled with 90mL of saline solution to prepare 10 -2 The dilution of (3) and so on. Are respectively prepared into 10 -3 、10 -4 、10 -5 The diluent of (4). Then respectively moving 10 by using a pipettor -3 、10 -4 、10 -5 100 μ L of the dilution was plated on nitrogen-fixing solid medium, and each gradient was repeated three times. Culturing in a constant-temperature biochemical incubator at 28 ℃ for 2-4 days, observing the growth condition of the bacteria every day, after single bacteria appear, selecting larger bacterial colonies by using an inoculating loop in an ultraclean workbench, inoculating the bacterial colonies to a BMS culture medium for purification, and after 5-6 generations of purification, preserving the bacterial colonies in 30% of glycerol at-80 ℃ for later use.
Example 2 identification of Strain NRCB005
The appearance of the strain is shown in figure 1.
The strain NRCB005 has higher homology with the bacillus amyloliquefaciens after BLAST comparison in NCBI through 16SrDNA sequence analysis, the similarity is 100 percent, and the strain NRCB005 is preliminarily identified as the bacillus amyloliquefaciens according to the appearance and physiological and biochemical characteristics. As can be seen from figure 1, the bacterial colony is round, opaque, rarely translucent, yellowish white and convex, has irregular edges and is easy to pick up.
The physiological and biochemical properties are shown in Table 1: the bacillus amyloliquefaciens NRCB005 starch hydrolysis, the V-P reaction, the gelatin liquefaction, the glucose acidogenesis and the lysozyme are all positive, and the methyl red test is negative.
TABLE 1 physiological and biochemical Properties of Strain NRCB005
| Strain numbering | Starch hydrolysis | V-P reaction | Methyl Red test | Liquefaction of gelatin | Acid production by glucose | Lysozyme |
| NRCB005 | + | + | - | + | + | + |
Note: "+" is positive and "-" is negative
EXAMPLE 3 determination of acid resistance of Bacillus amyloliquefaciens NRCB005
Adjusting the pH value of the liquid LB culture medium to 7, 6, 5, 4.5, 4, 3.5, 3 and 2.5 by using dilute hydrochloric acid and NaOH, and subpackaging in 250mL conical flasks, wherein each conical flask is subpackaged with 50mL; inoculating 100 mu L of bacterial liquid in logarithmic phase; 3 replicates per bacterium per salt concentration; the culture temperature is 30 ℃; shaking table 200rpm; culturing for 2-3 days; the OD600 value is measured, and the acid resistance of the strain is shown in figure 8.
As can be seen from FIG. 8, when the acidity of LB medium was greater than pH3.5, the OD600 of Bacillus amyloliquefaciens NRCB005 was 1.5 or more, but when the acidity of LB medium was less than pH3.5, the OD600 of Bacillus amyloliquefaciens NRCB005 was less than 0.3, and the above data indicate that Bacillus amyloliquefaciens NRCB005 had a good acid resistance at pH7 to pH3.5.
Example 4 plate bacteriostatic action of Bacillus amyloliquefaciens NRCB005 on major phytopathogens
Plate confrontation method: inoculating fusarium oxysporum, protomyces maydis and melon wilt pathogenic bacteria which are cultured on a PDA plate for 3-5 days to the center of a PDA culture medium, then inoculating bacillus amyloliquefaciens NRCB005 which is activated on the PDA culture medium for 24 hours to a position 2.5cm away from a bacterial cake, inoculating 1 point on each PDA plate, repeating for 3 times, and taking sterile water as a blank control. Culturing in 28 deg.C incubator, measuring the diameter of the colony of the treated group and the diameter of the control colony after the control pathogenic bacteria grow over the culture dish, and calculating the bacteriostasis rate, which is shown in Table 2. The bacterial strain NRCB005 has the rate of inhibiting pathogenic bacteria of melon wilt of 63%, the rate of inhibiting pathogenic bacteria of corn northern leaf blight of 58% and the rate of inhibiting pathogenic bacteria of fusarium oxysporum of 80%, and the bacterial strain NRCB005 can intuitively inhibit the growth of the pathogenic bacteria of melon wilt of melon, the pathogenic bacteria of corn northern leaf blight of corn and the pathogenic bacteria of fusarium oxysporum of fig. 3, 4 and 5, and has good biological control effect.
Inhibition (%) = (control colony diameter-treated colony diameter)/(control colony diameter) × 100%.
TABLE 2 bacteriostatic action of Bacillus amyloliquefaciens NRCB005 plate
Example 5 determination of production of volatile gas acetoin by Bacillus amyloliquefaciens NRCB005
Fermenting a seed culture medium: LB culture medium (yeast powder 5g/L, peptone 10g/L, naCl10 g/L) is adopted, and the culture conditions are that a rotary shaking table is at 28 ℃ and 200r/min.
A fermentation medium; 5g/L of yeast extract, 4g/L of corn steep liquor, 4g/L of urea and 10g/L of glucose, adjusting the pH value of the fermentation medium to 7, 6, 5, 4.5, 4, 3.5, 3 and 2.5 by using dilute hydrochloric acid and NaOH, culturing in a shaking table at the culture condition of 28 ℃ and 200r/min for 48 hours, and measuring the content of acetoin in the fermentation liquor by using gas chromatography.
Acetoin was analyzed by Agilent 7890A GC under the following GC conditions: hydrogen flame detector 220 deg.C, inlet temperature 210 deg.C, carrier gas N 2 And (4) temperature programming.
The acetoin producing ability of strain NRCB005 is shown in Table 3. As can be seen from the table, the strains NRCB005 have different acetoin secretion capacities in culture media with different acidity, and when the acidity of the culture media is pH7, the strains NRCB005 has the strongest acetoin secretion capacity, and the yield is 15g/L. FIG. 2 is a chromatographic detection result of a strain NRCB005 acetoin, wherein A is a 3g/L acetoin standard sample, B is a 1/30 48h fermentation liquid with acidity of pH7, the acetoin peak-off time in the standard sample is 6.177min, and an absorption peak appears at 6.180min in a sample chromatogram, which shows that the strain NRCB005 can produce acetoin.
Example 6 identification of the ability of Bacillus amyloliquefaciens NRCB005 to produce Indolylacetic acid (IAA)
The capability of NRCB002 to secrete IAA is measured by adopting a Salkowski colorimetric method.
Fermentation medium: 5g/L yeast powder, 10g/L peptone, 10g/L NaCl and 1g/L tryptophan. Inoculating the activated strain into a fermentation medium according to the inoculation amount of 1%, and adjusting the pH value of the fermentation medium to 7, 6, 5, 4.5, 4, 3.5, 3 and 2.5 by using dilute hydrochloric acid and NaOH. Culturing at 28 deg.C and 200r/min for 2-3 days, with 3 times of each strain. Centrifuging the fermentation liquor at 8000r/min for 5min, taking 1mL of supernatant into a 4mL centrifuge tube, adding 2mL of colorimetric solution, shaking up, placing for 15-20 min under the dark condition at room temperature, and measuring the light absorption value at 530 nm. Preparing IAA standard with IAA solution of different concentration, treating and measuring light absorption value by the same method as the sample, and drawing standard curve. The IAA content was determined with reference to a standard curve.
The Indole Acetic Acid (IAA) production by Bacillus amyloliquefaciens NRCB005 is shown in Table 3.
Example 7 analysis of phosphorus solubilizing Activity of Bacillus amyloliquefaciens NRCB005
Fermentation medium: KH (natural Kill) 2 PO 4 ，0.2g/L；K 2 HPO 4 ，0.8g/L；MgSO 4 ·7H 2 O，0.2g/L；Ca 3 (PO4) 2 5g/；NaCl，0.2g/L；CaSO 4 ·2H 2 O,0.1g/L; feCl3, trace; na (Na) 2 Mo 4 ·2H 2 0, trace; yeast extract, 0.5g/L; mannitol, 20g/L, fermenting with dilute hydrochloric acid and NaOHThe pH of the culture medium was adjusted to 7, 6, 5, 4.5, 4, 3.5, 3, 2.5. Inoculating the activated strain into a fermentation culture medium according to the inoculation amount of 1%, and culturing at 28 ℃ and 200r/min for 2-3 d, wherein each strain is cultured for 3 times. Centrifuging the fermentation liquor at 8000r/min, taking 5mL of supernatant into a 50mL volumetric flask, adding about 30mL of deionized water, adding 2 drops of dinitrophenol indicator, adjusting the solution to be yellowish by using sodium hydroxide and dilute sulfuric acid, then adding 5mL of molybdenum-antimony color-resisting reagent, shaking up, dissolving in a fixed amount, and carrying out color comparison at 700 nm.
The phosphorus solubilizing activity of Bacillus amyloliquefaciens NRCB005 is shown in Table 3.
Example 8 Potassium-solubilizing ability assay of Bacillus amyloliquefaciens NRCB005
Fermenting a seed culture medium: LB culture medium (yeast powder 5g/L, peptone 10g/L, naCl10 g/L) is adopted, and the culture conditions are that a rotary shaking table is at 28 ℃ and 200r/min.
Fermentation medium: 50g of sucrose (MgSO) 4 ·7H 2 O0.5g，CaCl 2 0.1g，FeCl 3 0.005g, potassium feldspar powder 1.0g and deionized water 1.0L, and the pH value of the fermentation medium is adjusted to 7, 6, 5, 4.5, 4, 3.5, 3 and 2.5 by using dilute hydrochloric acid and NaOH. After shaking culture for 3d under the culture condition of 28 ℃ and 200r/min, measuring the content of the quick-acting potassium in the fermentation liquor by using an atomic absorption spectrometer.
The potassium-solubilizing ability of Bacillus amyloliquefaciens NRCB005 is shown in Table 3.
As can be seen from Table 3, when the acidity of the strain NRCB005 fermentation liquor is pH7, the IAA and acetoin secretion amounts are the highest and are respectively 56mg/L and 15g/L, and the phosphorus dissolving amount and the potassium dissolving capacity are the strongest and respectively reach 82mg/L and 7.9mg/L. With the reduction of the pH value of the fermentation liquor, the IAA and acetoin secretion amount of the strain NRCB005 is gradually reduced, and the phosphorus dissolving amount and potassium dissolving capacity of the strain NRCB are gradually reduced.
TABLE 3 growth promoting Properties of Bacillus amyloliquefaciens NRCB005
Example 9 growth promotion of tomato by acetoin, an organic volatile gas, produced by fermentation of Bacillus amyloliquefaciens NRCB005
Inoculating the NRCB005 strain of the bacillus amyloliquefaciens into an LB liquid nutrient medium, culturing the strain to the middle logarithmic phase (OD 600= 1.0) at the temperature of between 25 and 30 ℃ under a shaking table of between 150 and 200r/min, centrifugally collecting the strain at the temperature of 4 ℃, and resuspending the strain by using sterile water for later use.
The surface disinfection of tomato seeds comprises the following steps: soaking in 70% ethanol for 15s, soaking in 2.5% sodium hypochlorite for 15min, washing with sterile water for 7-8 times, transferring tomato seeds to 1/2MS culture medium, and placing in 24 deg.C illumination incubator for germination acceleration for 2 days. Germinated tomato seeds were transferred to one chamber of 13 × 13cm petri dishes with septa, five plants were transplanted per dish, and 200 μ L of sterile water-suspended NRCB005 inoculum (OD 600= 1) was added to the other chamber of the medium. The medium was 1/2MS on both sides, and the acidity of the MS medium was adjusted to pH7 and pH4.2 with dilute hydrochloric acid and NaOH. 200 μ L of sterile water was used as a control. The culture dish is sealed by a sealing film and is placed in an illumination incubator with 24 ℃ and 16h illumination and 8h darkness for culture. After 7d of culture, the plant height, fresh weight, dry weight of the tomato were measured. As can be seen from the table, the Bacillus amyloliquefaciens NRCB005 can obviously increase the biomass of the tomatoes at the pH value of 7 and the pH value of 4.2, the Bacillus amyloliquefaciens NRCB005 has the most obvious effect on promoting the growth of the tomatoes at the acidity of 4.2, and the fresh weight and the dry weight of the tomatoes inoculated with the NRCB005 are respectively increased by 21 percent and 32 percent compared with the blank.
TABLE 4 growth promotion of tomato by organic volatile gas produced by Bacillus amyloliquefaciens NRCB005
Example 10 physicochemical Properties of the soil tested
The initial physicochemical properties of the soil to be tested were determined according to the soil agrochemical analysis method compiled by Lu Rukun, and are shown in table 5.
TABLE 5 physicochemical Properties of the soil tested
Example 11 growth-promoting Effect of Bacillus amyloliquefaciens NRCB005 on potted tomatoes
Inoculating Bacillus amyloliquefaciens NRCB005 into LB culture medium, shake culturing at 28 deg.C and 200r/min, measuring OD600 every 2 hr, centrifuging the bacterial liquid at 8000r/min when OD600 is 1, and collecting activated strain. The collected thalli is washed by 0.86% physiological saline, then is resuspended by using sterile water with the same volume, and is diluted by 100 times to prepare bacterial suspension for standby, and the blank is not inoculated with the bacillus amyloliquefaciens NRCB 005. Washing the tomato seeds with the same size and no damage with clear water, putting the tomato seeds into a culture dish filled with bacterial suspension to be tested, soaking for 2-3 h, transferring the tomato seeds into a flowerpot filled with soil to be tested, transferring 20 tomato seeds into each pot, sowing 4 pots in each treatment, and measuring the plant height, the leaf area and the fresh weight after growing for 30d under the natural illumination condition. The results are shown in Table 6. As can be seen from the table, in both non-acidified soil and acidified soil, the biomass of the tomatoes can be significantly increased by the Bacillus amyloliquefaciens NRCB005, and the fresh weight of the tomatoes inoculated with the NRCB005 are respectively increased by 52 percent and 45 percent compared with the blank. As is evident from FIGS. 6 and 7, the tomato inoculated with Bacillus amyloliquefaciens NRCB005 has higher plant height and stronger growth than the blank in both acidified soil and non-acidified soil, which shows that the Bacillus amyloliquefaciens NRCB005 has better capability of promoting the growth of the tomato in the acidified soil and the non-acidified soil.
The soil to be tested was the soil described in example 10, and the acidified soil was the soil in which the acidity of the non-acidified soil was adjusted to ph4.2 with dilute sulfuric acid.
TABLE 6 Bacillus amyloliquefaciens NRCB005 Pot culture experimental data
Sequence listing
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Claims (4)
1. Acid-resistant acetoin-producing bacillus amyloliquefaciens, which is classified and named as bacillus amyloliquefaciens (A)Bacillus amyloliquefaciens) The strain number is NRCB005, the strain is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, the preservation number is CGMCC NO.17214, and the preservation date is 2019, 1 month and 18 days.
2. The use of acid-tolerant acetoin-producing bacillus amyloliquefaciens according to claim 1 for the fermentative production of acetoin, said bacillus amyloliquefaciens having an acid tolerance of between pH7 and pH3.5.
3. Use of the acid-tolerant acetoin-producing bacillus amyloliquefaciens of claim 1 to promote plant growth in acidified soil;
wherein, the plant is tomato;
wherein, the acidified soil is soil with pH7.0-pH4.2.
4. Use of the acetoin-producing acid-resistant bacillus amyloliquefaciens of claim 1 to inhibit fusarium oxysporum, propamomyces zeae or fusarium oxysporum.
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| CN114436698B (en) * | 2022-02-17 | 2023-04-11 | 青岛力力惠生物科技股份有限公司 | Liquid compound microbial fertilizer and application thereof in agricultural production |
| CN114574401A (en) * | 2022-04-07 | 2022-06-03 | 南京工业大学 | Application of acetoin in improving rhizosphere growth-promoting bacteria in plant rhizosphere colonization |
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