CN109734821A - A kind of extracting method of natural bambo juice polysaccharide - Google Patents
A kind of extracting method of natural bambo juice polysaccharide Download PDFInfo
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- CN109734821A CN109734821A CN201811565221.8A CN201811565221A CN109734821A CN 109734821 A CN109734821 A CN 109734821A CN 201811565221 A CN201811565221 A CN 201811565221A CN 109734821 A CN109734821 A CN 109734821A
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- juice
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Abstract
The invention discloses a kind of extracting methods of natural bambo juice polysaccharide, this method includes taking juice, removal of impurities preservation, low temperature concentration, ethanol precipitation, deproteinization, secondary alcohol precipitation, drying and other steps, it extracts to obtain bamboo juice purity of polysaccharide height using method of the invention, purity is 80% ~ 85%, it is a kind of soluble polysaccharide of high-purity, and bamboo juice polysaccharide quality is stablized, maintain the effect of the original enhancing body's immunity of polysaccharide, the bamboo juice polysaccharide made has effects that potentially to build up health, life enhancement.
Description
Technical field
The present invention relates to a kind of Ethnic crude drugs deep process technology field, the extraction side of specifically a kind of natural bambo juice polysaccharide
Method.
Background technique
Bamboo juice is the water of life, living body water, very pure by the filtering, purification and activation of bamboo body itself, containing abundant
Nutrition and biological enzyme.It relieves the effect of alcohol for toxin expelling, is relieving cough and reducing sputum, aphthae, cough are against syndrome characterized by dyspnea, relieving restlessness and restlessness, the vomiting of pregnancy, stomach
The frightened pain of ulcer, sternal rib pain, feeling of oppression and fullness in the chest and epigastrium, appetizing of being amusing, children's wind-heat, especially has effects that anti-cancer and cancer-preventing.China and foreign countries scientist
Studies have shown that bamboo juice contains there are many precious microelement, such as silicon, zinc, selenium, germanium, 20 kinds of iron or more microelements and calcium,
A variety of macroelements such as phosphorus, magnesium, containing abundant vitamin, amino acid and polysaccharide, energy activating human body cells promote the new old generation of human body
It thanks, adjusts function of human body balance, keep human health, there is health-care physical therapy effect.Three pharmacological properties that China and foreign countries scientist separates
Composition, be respectively provided with treatment habitual constipation, hemostasis disappear hemorrhoid and blood pressure lowering, go dizziness and other effects.When big medical Lee scholar of the Ming Dynasty
Treasure is called " refreshing water ".Bamboo juice has an application in many fields now, such as cosmetics, food, functional beverage, Chinese medicine system
Agent etc..
21 century, medical model will change from therapeutic type to prevention type, and the mankind advocate back to nature.Therefore, it lifts in the world
The research climax to the active polysaccharide in natural food is played.Polysaccharide (polysaccharide) is the sugar combined by glycosidic bond
Chain at least will be more than the polymerization sugar polymeric carbohydrate of 10 monosaccharide compositions, can use general formula (C6H10O5) n expression.By
The polysaccharide of identical monosaccharide composition is known as homopolysaccharide, such as starch, cellulose and glycogen；It is known as with the polysaccharide that different monosaccharide forms
Heteroglycan, such as Arabic gum are made of pentose and galactolipin.Not a kind of pure chemical substance of polysaccharide, polymerize journey
Spend the mixture of different substances.Most of active polysaccharide it is swollen can to enhance reticuloendothelial system phagocytic with immune stimulating activity
The effect of oncocyte promotes lymphocyte transformation, activates T cell and B cell, and promote the formation of antibody.To in certain journey
There is antitumor activity on degree.But direct lethal effect is had no for tumour cell.Active polysaccharide can reduce methyl cholanthrene
The incidence of induced tumor should not take leukaemia, the lymph of operative treatment and radiotherapy to some easy generation transfers extensively
Tumor etc., it is especially valuable.When activity in man polysaccharide is sufficient, activity is high, active polysaccharide give full play to regulation, inhibition, reparation,
The effect of abnormality cell is activated, guarantees human health.When activity in man's polysaccharide is insufficient, activity reduces, active polysaccharide
Powerless regulation inhibits, repairs abnormality cell, and disease and aging just have occurred therewith.Active polysaccharide is supplemented to prevention and treatment heart and brain
Blood vessel, bone and joint diseases, endocrine system disease, disease of digestive tract, diabetes, hepatopathy, tuberculosis and tumour etc. have very heavy
The meaning wanted.
All very more to the research report of bamboo juice and polysaccharide, but the report that polysaccharide is extracted by bamboo juice is gone back at home now
It has no, the applicant is quasi- to study it, and plays its effect and be applied to some fields and benefit the society.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of natural bambo juice polysaccharide, extract to obtain bamboo juice using this method more
Sugared purity is high is a kind of soluble polysaccharide of high-purity, and bamboo juice polysaccharide quality is stablized, and the original enhancing body of polysaccharide is maintained
The effect of immune function, the bamboo juice polysaccharide made has effects that potentially to build up health, life enhancement.
A kind of extracting method of natural bambo juice polysaccharide, includes the following steps:
(1) take juice: choosing daily night 20:00 to morning next day 06:00 point, from the green bamboo of Guangxi province [Taiwan oldham bamboo,Dendrocalamopsis oldhami(Munro) Keng f.] outflow natural bambo juice.
(2) removal of impurities saves: obtained bamboo juice being filtered rapidly, insoluble impurities, then the bamboo juice that will be obtained are removed
Food grade plastic container after being packed into disinfection, is placed on 0~4 DEG C of refrigeration, spare.
(3) low temperature is concentrated: bamboo juice being placed in Rotary Evaporators, under 0.05~0.15MPa negative pressure, 40~60 DEG C of low temperature
Concentration, obtains 40 times of concentrates.
(4) ethanol precipitation: after mixing with volume ratio 1:8 by concentrate and ethyl alcohol, carrying out staticly settling 2~3h, to
Precipitation is centrifuged, and obtained sediment is dried under 40~60 DEG C of cryogenic conditions, obtains Thick many candies.
(5) deproteinization:
1) it weighs 3~5g of Thick many candies sample to be dissolved in water to 50mL, chloroform-isoamyl alcohol solution 20mL is added, is placed in separatory funnel
In fullyd shake, then stood, supernatant liquor collected after layering；
2) supernatant liquor being collected into is moved into separatory funnel again, 20mL chloroform-isoamyl alcohol solution is added, is sufficiently shaken
It swings, is then stood, supernatant liquor is collected after layering；
3) it is carried out again by step 2 extraction 1~2 time；Supernatant liquor after obtaining extraction 3~4 times.
(6) it secondary ethanol precipitation: after mixing with volume ratio 1:8 by supernatant liquor obtained by step (5) and ethyl alcohol, carries out
2~3h is staticly settled, precipitation to be precipitated is centrifuged, and sediment is dried under 40~60 DEG C of cryogenic conditions, obtains bamboo juice
Polysaccharide.
The volumetric concentration of the ethyl alcohol is 95%, and the volume ratio of the chloroform-isoamyl alcohol solution chloroform and isoamyl alcohol is 30-
45:8-10.
The purity is high of the bamboo juice polysaccharide, purity are 80% ~ 85%, are a kind of soluble polysaccharides of high-purity.
The beneficial effects of the present invention are:
In the present invention, the bamboo juice flowed out from the green bamboo of Guangxi province is selected, green bamboo is more in Guangxi province plantation amount, from green bamboo
Middle extraction natural bambo juice is at low cost and amount is more.Green bamboo is also referred to as oldham bamboo, more sweet by the bamboo juice after its filtering, purification and activation
Sweet tea, for extracting polysaccharide extract rate height.The present invention first cleans to bamboo juice, then carries out the de- egg of an alcohol precipitation, chloroform-isoamyl alcohol
White matter, secondary alcohol precipitation.Removal of impurities is filtered, insoluble impurities is first removed；Alcohol precipitation further removes the lipid in bamboo juice concentrate
And other impurities；Using chloroform-isoamyl alcohol solution deproteinization, chloroform not only can make protein denaturation that can also accelerate organic phase
And stratified liquid, isoamyl alcohol can reduce the bubble of protein degradation process generation and facilitate split-phase；Chloroform-isoamyl alcohol solution
It rests in chloroform-isoamyl alcohol, is stood after organic phase and stratified liquid after being denaturalized the protein component in Thick many candies,
Organic phase chloroform-isoamyl alcohol density is larger in lower layer, and polysaccharide collects supernatant liquor and carry out multiplicating extraction in upper phase
It takes, chloroform-isoamyl alcohol solution, which is repeatedly added, makes the protein in Thick many candies solution be removed more thorough, then carries out secondary alcohol
It is heavy, it can remove the new impurity that first time alcohol precipitation fails the impurity completely removed and generates in de- protein process, effectively mention
The high purity of polysaccharide.All it is to be dried using low temperature to polysaccharide after alcohol precipitation twice, is conducive to avoid high temperature that polysaccharide is caused to split
It solves and reduces yield.
The bamboo juice purity of polysaccharide extracted using method of the invention is high, and it is a kind of high-purity that purity, which is 80% ~ 85%,
Soluble polysaccharide, and bamboo juice polysaccharide quality is stablized, and collects bamboo juice under given conditions, is concentrated using Rotary Evaporators low temperature, it can
To effectively prevent polysaccharide to crack, the effect of the original enhancing body's immunity of polysaccharide is maintained, the bamboo juice polysaccharide tool made
There is the effect of potentially building up health, life enhancement.Extracting method through the invention can be by the effective component of bamboo juice with more
Add stable mode to store, and maintain the original health-care efficacy of bamboo juice, also further improving its effect makes its tool
There are some features of polysaccharide.Extracting method of the invention is simple, easy to operate, can also be according to the sample of restriction, solvent and molten
The usage ratio of liquid is multiplied dosage, is mass produced, obtained bamboo juice polysaccharide can be applied to bamboo juice beverage, Chinese medicine
The fields such as preparation, cosmetics.Method of the invention not only develops the new application of bamboo juice, also enriches the source of polysaccharide.
Specific embodiment
For the more detailed introduction present invention, below with reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of extracting method of natural bambo juice polysaccharide, includes the following steps:
(1) take juice: choosing daily night 20:00 to morning next day 06:00 point, from the green bamboo of Guangxi province [Taiwan oldham bamboo,Dendrocalamopsis oldhami(Munro) Keng f.] outflow natural bambo juice.
(2) removal of impurities saves: obtained bamboo juice being filtered rapidly, insoluble impurities, then the bamboo juice that will be obtained are removed
Food grade plastic container after being packed into disinfection, is placed on 0~4 DEG C of refrigeration, spare.
(3) low temperature is concentrated: bamboo juice 2000mL being placed in Rotary Evaporators, is concentrated under 0.1MPa negative pressure, 50 DEG C of low temperature
To 50mL, concentrate is obtained.
(4) ethanol precipitation: after mixing with volume ratio 1:8 by concentrate and ethyl alcohol, carrying out staticly settling 2h, to be precipitated
It is precipitated, is centrifuged, obtained sediment is dried under 50 DEG C of cryogenic conditions, obtains Thick many candies.
(5) deproteinization:
1) weigh Thick many candies sample 4g to be dissolved in water to 50mL, chloroform-isoamyl alcohol solution 20mL be added, be placed in separatory funnel into
Row fullys shake, and is then stood, supernatant liquor is collected after layering；
2) supernatant liquor for being again collected into step 1) moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering；
3) supernatant liquor for being again collected into step 2 moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering.
(6) it secondary ethanol precipitation: after mixing with volume ratio 1:8 by supernatant liquor obtained by step (5) and ethyl alcohol, carries out
3h is staticly settled, precipitation to be precipitated is centrifuged, and sediment is dried under 50 DEG C of cryogenic conditions, obtains bamboo juice polysaccharide.
The volumetric concentration of the ethyl alcohol is 95%, and the volume ratio of the chloroform-isoamyl alcohol solution chloroform and isoamyl alcohol is 38:
9。
The purity is high of the bamboo juice polysaccharide, purity are 80% ~ 85%, are a kind of soluble polysaccharides of high-purity.
Embodiment 2
A kind of extracting method of natural bambo juice polysaccharide, includes the following steps:
(1) take juice: choosing daily night 20:00 to morning next day 06:00 point, from the green bamboo of Guangxi province [Taiwan oldham bamboo,Dendrocalamopsis oldhami(Munro) Keng f.] outflow natural bambo juice.
(2) removal of impurities saves: obtained bamboo juice being filtered rapidly, insoluble impurities, then the bamboo juice that will be obtained are removed
Food grade plastic container after being packed into disinfection, is placed on 0~4 DEG C of refrigeration, spare.
(3) low temperature is concentrated: bamboo juice being placed in Rotary Evaporators, is concentrated under 0.15MPa negative pressure, 40 DEG C of low temperature, obtains 40
Times concentrate.
(4) ethanol precipitation: after mixing with volume ratio 1:8 by concentrate and ethyl alcohol, carrying out staticly settling 2h, to be precipitated
It is precipitated, is centrifuged, obtained sediment is dried under 60 DEG C of cryogenic conditions, obtains Thick many candies.
(5) deproteinization:
1) weigh Thick many candies sample 3g to be dissolved in water to 50mL, chloroform-isoamyl alcohol solution 20mL be added, be placed in separatory funnel into
Row fullys shake, and is then stood, supernatant liquor is collected after layering；
2) supernatant liquor for being again collected into step 1) moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering；
3) supernatant liquor for being again collected into step 2 moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering.
(6) it secondary ethanol precipitation: after mixing with volume ratio 1:8 by supernatant liquor obtained by step (5) and ethyl alcohol, carries out
3h is staticly settled, precipitation to be precipitated is centrifuged, and sediment is dried under 40 DEG C of cryogenic conditions, obtains bamboo juice polysaccharide.
The volumetric concentration of the ethyl alcohol is 95%, and the volume ratio of the chloroform-isoamyl alcohol solution chloroform and isoamyl alcohol is 30:
8。
The purity is high of the bamboo juice polysaccharide, purity are 80% ~ 85%, are a kind of soluble polysaccharides of high-purity.
Embodiment 3
A kind of extracting method of natural bambo juice polysaccharide, includes the following steps:
(1) take juice: choosing daily night 20:00 to morning next day 06:00 point, from the green bamboo of Guangxi province [Taiwan oldham bamboo,Dendrocalamopsis oldhami(Munro) Keng f.] outflow natural bambo juice.
(2) removal of impurities saves: obtained bamboo juice being filtered rapidly, insoluble impurities, then the bamboo juice that will be obtained are removed
Food grade plastic container after being packed into disinfection, is placed on 0~4 DEG C of refrigeration, spare.
(3) low temperature is concentrated: bamboo juice being placed in Rotary Evaporators, is concentrated under 0.05MPa negative pressure, 60 DEG C of low temperature, obtains 40
Times concentrate.
(4) ethanol precipitation: after mixing with volume ratio 1:8 by concentrate and ethyl alcohol, carrying out staticly settling 3h, to be precipitated
It is precipitated, is centrifuged, obtained sediment is dried under 40 DEG C of cryogenic conditions, obtains Thick many candies.
(5) deproteinization:
1) weigh Thick many candies sample 5g to be dissolved in water to 50mL, chloroform-isoamyl alcohol solution 20mL be added, be placed in separatory funnel into
Row fullys shake, and is then stood, supernatant liquor is collected after layering；
2) supernatant liquor for being again collected into step 1) moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering；
3) supernatant liquor for being again collected into step 2 moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering；
4) supernatant liquor for being again collected into step 3) moves into separatory funnel, and 20mL chloroform-isoamyl alcohol solution is added, is filled
Divide concussion, is then stood, supernatant liquor is collected after layering.
(6) it secondary ethanol precipitation: after mixing with volume ratio 1:8 by supernatant liquor obtained by step (5) and ethyl alcohol, carries out
2h is staticly settled, precipitation to be precipitated is centrifuged, and sediment is dried under 60 DEG C of cryogenic conditions, obtains bamboo juice polysaccharide.
The volumetric concentration of the ethyl alcohol is 95%, and the volume ratio of the chloroform-isoamyl alcohol solution chloroform and isoamyl alcohol is 45:
10。
The purity is high of the bamboo juice polysaccharide, purity are 80% ~ 85%, are a kind of soluble polysaccharides of high-purity.
The basic principles, main features and advantages of the present invention have been shown and described above.The present invention is not by upper
The limitation of embodiment is stated, the above embodiments and description only illustrate the principle of the present invention, is not departing from the present invention
Various changes and improvements may be made to the invention under the premise of spirit and scope, these changes and improvements both fall within that the present invention claims guarantors
In the range of shield.The scope of protection of present invention is defined by appended claims and its equivalent.
Claims (4)
1. a kind of extracting method of natural bambo juice polysaccharide, which comprises the steps of:
(1) it takes juice: choosing the natural bambo juice flowed out from bamboo living；
(2) removal of impurities saves: obtained bamboo juice being filtered rapidly, removes insoluble impurities, then obtained bamboo juice is packed into
Food grade plastic container after disinfection is placed on 0~4 DEG C of refrigeration, spare；
(3) low temperature is concentrated: bamboo juice is placed in Rotary Evaporators, it is dense under 0.05~0.15MPa negative pressure, 40~60 DEG C of low temperature
Contracting, obtains 40 times of concentrates；
(4) ethanol precipitation: after mixing with volume ratio 1:8 by concentrate and ethyl alcohol, carrying out staticly settling 2~3h, to be precipitated
It is precipitated, is centrifuged, obtained sediment is dried under 40~60 DEG C of cryogenic conditions, obtains Thick many candies；
(5) deproteinization:
1) it weighs 3~5g of Thick many candies sample to be dissolved in water to 50mL, 20 mL of chloroform-isoamyl alcohol solution is added, is placed in separatory funnel
In fullyd shake, then stood, supernatant liquor collected after layering；
2) supernatant liquor being collected into is moved into separatory funnel again, 20 mL chloroform-isoamyl alcohol solution is added, are sufficiently shaken
It swings, is then stood, supernatant liquor is collected after layering；
3) it is carried out again by step 2 extraction 1~2 time；Supernatant liquor after obtaining extraction 3~4 times；
(6) it secondary ethanol precipitation: after mixing with volume ratio 1:8 by supernatant liquor obtained by step (5) and ethyl alcohol, is stood
2~3h is precipitated, precipitation to be precipitated is centrifuged, sediment is dried under 40~60 DEG C of cryogenic conditions, it is more to obtain bamboo juice
Sugar.
2. the extracting method of natural bambo juice polysaccharide according to claim 1, which is characterized in that natural in the step (1)
Bamboo juice takes the juice time are as follows: daily night 20:00 to morning next day 06:00 point；Selected bamboo living is the green bamboo of Guangxi province.
3. the extracting method of natural bambo juice polysaccharide according to claim 1, which is characterized in that the volumetric concentration of the ethyl alcohol is
95%, the volume ratio of the chloroform-isoamyl alcohol solution chloroform and isoamyl alcohol is 30-45:8-10.
4. the extracting method of natural bambo juice polysaccharide according to claim 1, which is characterized in that the purity of the bamboo juice polysaccharide
Height, purity are 80% ~ 85%, are a kind of soluble polysaccharides of high-purity.
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Citations (7)
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| CN1927066A (en) * | 2006-09-28 | 2007-03-14 | 邓天华 | Processing method for natural bamboo juice beverage |
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| CN102911287A (en) * | 2012-11-21 | 2013-02-06 | 福建科宏生物工程有限公司 | Method for preparing bamboo leaf polysaccharides from bamboo pulp |
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| CN105085698A (en) * | 2015-08-12 | 2015-11-25 | 华南理工大学 | Extraction method for oxidation-resistive bamboo leaf polysaccharide |
-
2018
- 2018-12-20 CN CN201811565221.8A patent/CN109734821A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1287848A (en) * | 1999-09-09 | 2001-03-21 | 李雪涛 | Method for extracting nutrition essence from bamboo, and series products thereof |
| JP2005526148A (en) * | 2001-08-03 | 2005-09-02 | レイヨニアー プロダクツ アンド フィナンシァル サービシズ カンパニー | Highly carboxylated cellulose fiber and method for producing the same |
| CN1927066A (en) * | 2006-09-28 | 2007-03-14 | 邓天华 | Processing method for natural bamboo juice beverage |
| CN101392032A (en) * | 2008-11-05 | 2009-03-25 | 中国林业科学研究院林产化学工业研究所 | Method for preparing bamboo leaf polysaccharide extract by member separation |
| CN102911287A (en) * | 2012-11-21 | 2013-02-06 | 福建科宏生物工程有限公司 | Method for preparing bamboo leaf polysaccharides from bamboo pulp |
| CN104017095A (en) * | 2013-03-01 | 2014-09-03 | 浙江大学 | Method for extracting bamboo polysaccharides |
| CN105085698A (en) * | 2015-08-12 | 2015-11-25 | 华南理工大学 | Extraction method for oxidation-resistive bamboo leaf polysaccharide |
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Application publication date: 20190510 |