CN109734614B - 3-hydroxy chalcone Mannich base compound, preparation method and application thereof - Google Patents

Abstract

The invention discloses a novel 3-hydroxychalcone Mannich base compound (I) and pharmaceutically acceptable salts thereof, a preparation method thereof, a pharmaceutical composition and application thereof in preparing medicaments for treating and/or preventing related diseases of a nervous system, wherein the related diseases include but are not limited to vascular dementia, Alzheimer disease, Parkinson disease, Huntington disease, HIV-related dementia disease, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma, ischemic stroke, hemorrhagic stroke, nerve injury caused by brain trauma and other diseases;

Description

3-hydroxy chalcone Mannich base compound, preparation method and application thereof

Technical Field

The invention belongs to the field of medicinal chemistry, and relates to a novel 3-hydroxychalcone Mannich base compound (I) and pharmaceutically acceptable salts thereof, a preparation method thereof, a medicinal composition and application thereof in preparing medicaments for treating and/or preventing related diseases of a nervous system, wherein the diseases comprise vascular dementia, Alzheimer disease, Parkinson disease, Huntington disease, HIV-related dementia, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma, ischemic stroke, hemorrhagic stroke, nerve injury caused by brain trauma and the like.

Background

Neurodegenerative diseases refer to a general term for diseases caused by chronic progressive degeneration of central nervous tissue, including Alzheimer's Disease (AD), Parkinson's Disease (PD), Huntington's Disease (HD), Amyotrophic Lateral Sclerosis (ALS), and Multiple Sclerosis (MS), and the pathogenesis of which is closely related to oxidative stress, neuroinflammation, and corresponding injury. Oxidative stress is mediated by Reactive Oxygen Species (ROS) radicals, including superoxide anions, hydrogen peroxide, and hydroxyl radicals, among others. Under normal physiological conditions, the ROS production level and the body antioxidant capacity are in a dynamic balance state, when the ROS production exceeds the cell antioxidant capacity, Oxidative stress (Oxidative stress) occurs, and the brain is particularly sensitive to the Oxidative stress, so that various nervous system diseases are induced. In addition, researches show that vascular dementia, HIV-related dementia, neuropathic pain, glaucoma, ischemic stroke, hemorrhagic stroke, nerve injury caused by brain trauma and the like are also related to oxidative stress and neuroinflammation of the body.

Vascular Dementia (VD) is a clinical syndrome of intellectual and cognitive dysfunction caused by various types of cerebrovascular diseases, including ischemic cerebrovascular diseases, hemorrhagic cerebrovascular diseases, acute and chronic hypoxic cerebrovascular diseases, etc. Due to the complex pathogenesis of vascular dementia, no medicine capable of blocking the disease development exists at present, and the clinical treatment mainly aims at improving the brain blood circulation and brain metabolism and strengthening the brain nutrition. Recent studies have shown that VD patients often have abnormalities in the cholinergic system as well as impaired cognitive function. The density of ChAT positive neurons and fibers in the hippocampal region of a VD patient is reduced, the ChAT activity of different parts in the brain is reduced, the concentration of acetylcholine in cerebrospinal fluid of the VD patient is obviously lower than the normal level, and the degree of reduction of the concentration is positively correlated with the severity of dementia; cerebral ischemia can cause the activity of acetylcholinesterase in brain to rise; meanwhile, the acetylcholinesterase inhibitors are found to protect neuron damage caused by ischemia and promote nerve damage and brain function recovery after cerebral ischemia.

Alzheimer's disease (senile dementia) is a degenerative disease of the central nervous system mainly involving progressive cognitive impairment and memory impairment, and its incidence rate is increasing year by year, and it is a high-grade disease next to cardiovascular diseases and cancer, and it is the fourth cause of death in developed countries such as europe and the united states. With the accelerated aging process of the global population, the incidence rate of the Disease is in a clear rising trend, and according to the global influence of Alzheimer's Disease published in 2013 in 12 months by Alzheimer's Disease International: 2013-2050 reports indicate that AD will become the biggest health challenge worldwide for decades in the future, and by 2030, the number of patients will rise from 4400 ten thousand in 2013 to 7600 ten thousand, and by 2050, the number will reach 1.35 hundred million which is surprising. Because AD is clinically manifested as hypomnesis, orientation ability, thinking and judgment ability, reduction of daily life ability, even abnormal mental behavior symptoms, and the like, the nursing difficulty of patients is large, and the heavy burden is brought to the society and families. Currently approved drugs for the treatment of light/moderate AD are acetylcholinesterase (AChE) inhibitors, and for the treatment of severe ADN-methyl-D-an aspartate (NMDA) receptor antagonist. Clinical application shows that the medicines can relieve AD symptoms by improving the acetylcholine level in a patient body or inhibiting excitotoxicity of excitatory amino acid, but cannot effectively prevent or reverse the course of disease, and can cause severe toxic and side effects such as hallucinations, consciousness chaos, dizziness, headache, nausea, hepatotoxicity, inappetence, frequent defecation and the like, so that the long-term curative effect is not ideal. Therefore, there is an urgent clinical need to develop a novel therapeutic agent for AD that has both improved symptoms and altered course of disease.

AD is a disease caused by various factors, the pathogenesis of the AD is complex, and the pathogenesis of the AD is not completely clarified so far. However, studies have shown that the patient has a decreased acetylcholine level in the brain,βOverproduction and deposition of amyloid, platelet aggregation in cerebral vessels, metabolic disorders of metal ions, Ca²⁺Loss of balanceRegulating,tauNeurofibrillary tangles caused by protein hyperphosphorylation, glutamate receptor hyperactivity, large amounts of Reactive Oxygen Species (ROS) and free radicals produced by oxidative stress, and various factors such as neuroinflammatory responses play important roles in the pathogenesis of AD. In view of the above pathogenic factors, researchers have found a large number of drugs with high activity and high selectivity to a target by using the traditional "one drug one target" drug design strategy, such as: cholinesterase inhibitors andN-methyl-DAspartate receptor antagonists and the like. However, the drugs have the problems of single action target, more toxic and side effects in clinical use, poor long-term curative effect on AD patients and the like.

In recent years, with the continuous elucidation of the pathogenic mechanism of AD, the occurrence and development of AD have the characteristics of multi-mechanism and multi-factor action, and different mechanisms are mutually associated and influenced to form a complex network regulation and control system in the occurrence and development process of AD. Obviously, the development of therapeutic drugs that can act simultaneously on multiple links in the pathological process of AD is the current necessity. Based on the above results, researchers have proposed a "multi-target-directed drugs" (MTDLs) strategy to develop anti-neurodegenerative drugs. By "multi-target drug" is meant that a single chemical entity acts on multiple targets in a disease network simultaneously, and the effect on each target can produce a synergistic effect, such that the total effect is greater than the sum of the individual effects, such compounds also being referred to as "Multifunctional" or "Multipotential" drugs. The main differences of the multi-target point medicine and the multi-medicine combined application and the compound medicine are as follows: can reduce the dosage, improve the treatment effect, avoid the interaction between the medicaments and the toxic and side effect caused by the interaction, have uniform pharmacokinetic characteristic, and are convenient to use, and the like. Therefore, the research and development of the neurodegenerative disease resisting treatment drug which has a novel chemical structure, a novel action mechanism, a multi-target effect and low toxic and side effects not only meets the urgent need of the social aging process, but also has good market prospect. A large number of clinical studies have proved that AChE inhibitors can effectively relieve the symptoms of AD patients, and the short-term treatment effect is positive; therefore, it is often necessary to retain the AChE inhibition of a compound in the design of multi-target anti-AD drugsInhibiting the activity of the enzyme (inhibiting the enzyme is important for improving the symptoms of AD patients) and increasing one or more other targets or functions with pharmacological synergistic effect on the basis of the activity so as to achieve the multi-target AD treatment effect. Obviously, the design and the discovery have the effects of inhibiting acetylcholinesterase and inhibitingβThe overproduction and deposition of amyloid, antioxidant stress and multi-target AD therapeutics against monoamine oxidase-B remain important research directions at present.

Disclosure of Invention

The invention aims to disclose a novel 3-hydroxychalcone Mannich base compound (I) and a pharmaceutically acceptable salt thereof.

The invention also aims to disclose a preparation method of the 3-hydroxychalcone Mannich base compound (I) and pharmaceutically acceptable salts thereof.

The invention also aims to disclose a pharmaceutical composition containing the 3-hydroxychalcone Mannich base compound (I) and a pharmaceutically acceptable salt thereof.

The invention also aims to disclose that the 3-hydroxychalcone mannich base compound (I) and the pharmaceutically acceptable salt thereof have multi-target effect, and can be used for preparing the medicines for treating and/or preventing related diseases of the nervous system, such as vascular dementia, alzheimer disease, parkinson disease, huntington disease, HIV-related dementia disease, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma, ischemic stroke, hemorrhagic stroke, nerve injury caused by brain trauma and the like.

The general chemical structure formula of the 3-hydroxychalcone Mannich base compound (I) provided by the invention is as follows:

in the formula: r₁、R₂And R₃Each independently represents H, OH, CF₃O、C₁~C₁₂Alkoxy, NR₆R₇However, R₁、R₂And R₃Not simultaneously represent H, R₄And R₅Each independently represents C₁~C₁₂Alkyl, benzyl, substituted benzyl, R₆And R₇Each independently represents C₁~C₁₂Alkyl radical, NR₄R₅Also represents tetrahydropyrrolyl, morpholinyl, piperidinyl, piperazinyl, 4-position by C₁~C₁₂Piperazinyl substituted by alkyl, piperazinyl substituted in the 4-position by benzyl or substituted benzyl, NR₆R₇Also represents tetrahydropyrrolyl, piperidinyl, R₁、R₂、R₃At any possible position on the corresponding phenyl ring; however, the 3-hydroxychalcone mannich base compound (I) does not represent a compound represented by the following general formula:

NR₈R₉represents N (CH)₃)₂Tetrahydropyrrolyl, piperidinyl, morpholinyl,N-methylpiperazinyl radical,N-an ethyl piperazinyl group;

the "substituted benzyl" refers to a benzyl group substituted on the phenyl ring with 1 to 4 groups selected from the group consisting of: F. cl, Br, I, C_1-4Alkyl radical, C_1-4Alkoxy, trifluoromethyl, trifluoromethoxy, dimethylamino, these substituents being in any possible position on the phenyl ring of the benzyl group.

The 3-hydroxychalcone Mannich base compound (I) provided by the invention can be prepared by the following method:

in the formula: r₁～R₅The definition of (A) is the same as that of the chemical structural general formula of the 3-hydroxy chalcone Mannich base compound (I).

Corresponding substituted acetophenone compound (1) and 3-hydroxybenzaldehyde Mannich base compound (2) are taken as initial raw materialsDirectly condensing under the conditions of solvent and alkali to obtain the corresponding 3-hydroxychalcone Mannich base compound (I). Wherein the alkali used in the reaction is: alkali metal hydroxide, alkaline earth metal hydroxide, alkali metal carbonate, alkaline earth metal carbonate, alkali metal bicarbonate, alkaline earth metal bicarbonate, C_1-8Alkali metal salts of alcohols, organic tertiary or quaternary amines (e.g. triethylamine, tributylamine, trioctylamine, pyridine, tert-butyl amine, pyridine, tert-butyl amine, pyridine, tert-butyl amine, pyridine, tert-butyl amine, tert-butyl,N-methylmorpholine,NMethylpiperidine, triethylenediamine, tetrabutylammonium hydroxide), the preferred bases being: potassium hydroxide, sodium hydroxide, potassium carbonate, triethylamine or pyridine; the solvent used in the reaction is: c_1-8Fatty alcohol, diethyl ether, tetrahydrofuran, 2-methyltetrahydrofuran,N,N-dimethylformamide, dimethylsulfoxide, dichloromethane, chloroform, 1, 4-dioxane, benzene, toluene or acetonitrile, preferably in the presence of a solvent: methanol, ethanol, isopropanol,N,N-dimethylformamide, acetonitrile, tetrahydrofuran, dichloromethane or toluene; substituted acetophenone compound (1): 3-hydroxybenzaldehyde mannich base compounds (2): the molar charge ratio of alkali is 1.0: 1.0-3.0: 1.0-20.0, and preferably, the molar feed ratio is 1.0: 1.0-2.0: 1.0 to 10.0; the reaction temperature is 0-150 ℃, and the preferable reaction temperature is room temperature-120 ℃; the reaction time is 1-120 hours, and the preferable reaction time is 2-72 hours.

The starting materials of the invention, namely the substituted acetophenone compound (1) and the 3-hydroxybenzaldehyde Mannich base compound (2), can be prepared by the technique which is common in the field, namely: the compound is prepared by taking corresponding 3-hydroxybenzaldehyde as a substrate and performing conventional Mannich reaction with formaldehyde (or paraformaldehyde) and a corresponding secondary amine compound under the catalysis of acid.

The 3-hydroxychalcone mannich base compound (I) obtained by the above method contains amino groups in the molecule, the amino groups are basic, and can be prepared into pharmaceutically acceptable salts thereof by a pharmaceutically conventional salt forming method with any suitable acid, wherein the acid is: hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid, sulfamic acid, C_1-6Aliphatic carboxylic acids (e.g. formic acid, acetic acid, propionic acid, etc.), trifluoroacetic acid, stearic acid, pamoic acid, oxalic acid, benzoic acid,Phenylacetic acid, salicylic acid, maleic acid, fumaric acid, succinic acid, tartaric acid, citric acid, malic acid, lactic acid, hydroxymaleic acid, pyruvic acid, glutamic acid, ascorbic acid, lipoic acid, C_1-6Alkyl sulfonic acids (e.g., methanesulfonic acid, ethanesulfonic acid, etc.), camphorsulfonic acid, naphthalenesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, or 1, 4-butanedisulfonic acid.

The pharmaceutical composition disclosed by the invention comprises one or more 3-hydroxychalcone Mannich base compounds (I) or pharmaceutically acceptable salts thereof with a therapeutically effective amount, and the pharmaceutical composition can further contain one or more pharmaceutically acceptable carriers or excipients. The "therapeutically effective amount" refers to the amount of a drug or agent that elicits a biological or medicinal response in a tissue, system, or animal targeted by a researcher or physician; the term "composition" refers to a product formed by mixing more than one substance or component; the "pharmaceutically acceptable carrier" refers to a pharmaceutically acceptable substance, composition or vehicle, such as: liquid or solid fillers, diluents, excipients, solvents or encapsulating substances, which carry or transport certain chemical substances. The ideal proportion of the pharmaceutical composition provided by the invention is that the 3-hydroxychalcone Mannich base compound (I) or the pharmaceutically acceptable salt thereof is taken as an active ingredient and accounts for 5-99.5% of the total weight, and the rest accounts for less than 95% of the total weight.

The 3-hydroxychalcone Mannich base compound (I) and pharmaceutically acceptable salts thereof disclosed by the invention are subjected to the following biological activity screening.

(1) 3-hydroxy chalcone Mannich base compound (I) has inhibitory activity on acetylcholinesterase and butyrylcholinesterase

To a 96-well plate, 30. mu.L of thioacetylcholine iodide or thiobutyrylcholine iodide (each obtained from Sigma), 40. mu.L of PBS buffer solution at pH7.4, 20. mu.L of a test compound solution (DMSO content: less than 1%), and 10. mu.L of acetylcholinesterase (5% homogenate supernatant of rat brain cortex, phosphate buffer solution at pH7.4 as homogenization medium) or butyrylcholinesterase (25% supernatant of rat serum, pH7.4 phosphate buffer solution as homogenization medium) were added in this order, and then addedAfter mixing, incubating at 37 ℃ for 15min, adding 0.2% 5, 5' -dithio-bis (2-nitrobenzoic acid) (DTNB, purchased from Sigma) solution 30. mu.L to each well for color development, measuring the optical density (OD value) of each well at 405nm by using an enzyme labeling instrument, and calculating the inhibition rate of the compound on the enzyme (enzyme inhibition rate (%) = (1-sample group OD value/blank group OD value) × 100%) compared with a blank well without the sample to be measured); selecting five to six concentrations of the compound, measuring the enzyme inhibition rate, performing linear regression by using the negative logarithm of the molar concentration of the compound and the enzyme inhibition rate, and obtaining the molar concentration when the 50% inhibition rate is obtained as the IC of the compound₅₀. The determination result shows that the 3-hydroxychalcone Mannich base compound (I) disclosed in the embodiment of the invention has obvious inhibition effect on acetylcholinesterase and IC (integrated Circuit) thereof₅₀The particle size is 10.2 nM-12.0 [ mu ] M; and the inhibitory activity of the 3-hydroxychalcone Mannich base compound (I) on acetylcholinesterase is obviously higher than that of butyrylcholinesterase (the selectivity is more than 10 times), which shows that the compound disclosed by the invention has a selective inhibitory effect on acetylcholinesterase. Further structure-activity relationship research shows that in the chemical structural general formula of the 3-hydroxychalcone Mannich base compound (I), when R is₁、R₂And R₃Meanwhile, the compound also represents H, and the definition of other substituent groups is the same as the general formula of the chemical structure, the acetylcholinesterase inhibitory activity of the compound is greatly reduced (IC)₅₀Are all larger than 60 mu M); and the mother nucleus of the 3-hydroxy chalcone Mannich base compound (I) -3-hydroxy chalcone compound [ R₁、R₂、R₃Is as defined for the chemical structural formula of compound (I), CH₂NR₄R₅Indicating that H and OH are located at position 3, and 3-hydroxybenzaldehyde Mannich base compound (2) IC for acetylcholinesterase inhibition₅₀Are all larger than 100 mu M. In addition, the experiment also finds that the 3-OH and 4-CH in the structural general formula of the 3-hydroxychalcone Mannich base compound (I)₂NR₄R₅After the substitution positions are interchanged, the obtained corresponding compounds have obviously reduced inhibitory activity to acetylcholinesterase and corresponding IC₅₀Ratio of values (Compound IC after interchange)₅₀Value/corresponding Compound (I) IC₅₀Value) is at least greater than 3.0.

(2) Antioxidant activity of 3-hydroxychalcone Mannich base compounds (I) (ORAC-FL method)

Reference (Qiang, X.M.et al.Eur. J Med. Chem.2014, 76, 314-: 6-hydroxy-2, 5,7, 8-tetramethylchromane-2-carboxylic acid (C)Trolox) The solution was adjusted to 10-80. mu. mol/L with PBS buffer solution of pH7.4, the solution was adjusted to 250 nmol/L with PBS buffer solution of pH7.4 for fluorescein (fluorescein), and the solution was adjusted to 40 mmol/L with PBS buffer solution of pH7.4 for 2, 2' -azobisisobutylamidine dihydrochloride (AAPH) before use. The compound solution and the fluorescein solution were added to a 96-well plate at 50-10. mu. mol/L, mixed, incubated at 37 ℃ for 15min, and AAPH solution was added to make the total volume per well 200. mu.L, mixed, immediately placed in a Varioskan Flash Multimode Reader (Thermo Scientific) instrument, and continuously measured at 485 nm excitation wavelength and 535 nm emission wavelength for 90 min. Calculating the area AUC under the fluorescence decay curve, wherein the area AUC is 1-8 mu mol/LTroloxAs a standard, taking a sample not to be tested as a blank, and expressing the antioxidant activity result of the compound asTroloxThe formula of the equivalent of (a) is: [ (AUC Sample-AUC blank)/(AUCTrolox-AUC blank)] ×[(concentration of Trolox/concentration of sample)]Each compound was assayed in 3 replicates each, each set of experiments was independently repeated three times. The determination result shows that the antioxidant activity of the 3-hydroxychalcone Mannich base compound (I) disclosed in the embodiment of the invention isTrolox0.96-3.0 times of the total amount of the compound, which shows that the compound has strong antioxidant activity.

(3) 3-hydroxy chalcone Mannich base compound (I) for Ab _1-42Inhibitory Activity of self-aggregation

Reference (Qiang, X.M.et al.Eur. J Med. Chem.2014, 76, 314-: pretreated Aβ _1-42Stock solutions were prepared in DMSO, and diluted to 50. mu.M in PBS buffer, pH7.4, before use; the test compound was prepared in 2.5 mM stock solution in DMSO,before use, the mixture was diluted to a corresponding concentration with PBS buffer (pH7.4), and 20. mu.L of A was takenβ _1-42Solution + 20. mu.L of test Compound solution, 20. mu.L of Aβ _1-42Solution +20 μ L PBS buffer (containing 2% DMSO) in 96-well plates, incubated at 37 ℃ for 24h, then 160 μ L of 50mM glycine-NaOH buffer (pH = 8.5) containing 5 μ M thioflavin T was added, and fluorescence was measured immediately after shaking for 5s with a multifunctional plate reader at 446 nm excitation wavelength and 490 nm emission wavelength; a. theβ _1-42+ the fluorescence value of the test compound is recorded as IF_i，Aβ _1-42The fluorescence value of + PBS buffer was designated as IF_cThe fluorescence value of the buffer solution containing only PBS was designated as IF₀Compounds inhibiting Aβ _1-42The inhibition rate of self-aggregation is: 100- (IF)_i-IF₀)/(IF_c-IF₀) 100, x; five to six concentrations of compound were selected and their inhibition was determined, three replicates per compound concentration, with curcumin as a positive control. The determination result shows that the 3-hydroxy chalcone Mannich base compound (I) disclosed in the embodiment of the invention is opposite to Aβ _1-42The self-aggregation has obvious inhibitory activity on A at the concentration of 25.0 mu Mβ _1-42The inhibition rate of self-aggregation is more than 40.0 percent; the inhibition rate of curcumin under the same concentration is 41.3%, and the anti-AD drugs which are widely used clinically: donepezil, rivastigmine, memantine hydrochloride, 3-hydroxy chalcone (R) as parent nucleus of compound (I)₁、R₂、R₃And CH₂NR₄R₅All indicate that H and OH are positioned at 3-position), and the 3-hydroxybenzaldehyde Mannich base compound (2) is applied to A under the concentration of 25.0 mu Mβ _1-42The inhibition rate of self-aggregation is less than 15 percent.

(4) Inhibitory activity of 3-hydroxychalcone Mannich base compound (I) on monoamine oxidase A and B

Recombinant human MAO-A was prepared as A sample solution at 12.5. mu.g/mL using 100 mM potassium phosphate buffer pH7.4, and MAO-B was prepared as A sample solution at 75. mu.g/mL. Adding 20 μ L of the compound solution to be detected and 80 μ L of monoamine oxidase into a black 96-well plate, mixing, incubating at 37 deg.C in dark place for 15min, and adding 200 μ M Amplex RA ed reagent, 2U/mL horseradish peroxidase, 2 mM p-hydroxyphenylethylamine (inhibiting MAO-A) or 2 mM phenylmethylamine (inhibiting MAO-B) to initiate A reaction, incubating for 20 min at 37 ℃, measuring the fluorescence emission intensity at 590 nm on A multifunctional enzyme-linked immunosorbent assay (ELISA) instrument by using A fixed excitation wavelength of 545 nm, and using potassium phosphate buffer solution to replace MAO-A or MAO-B as A blank; the inhibition rate of the compound for inhibiting monoamine oxidase is calculated by the following formula: 100- (IF)_i)/(IF_c) 100 of the formula, IF_iAnd IF_cThe difference between the fluorescence intensity in the presence and absence of inhibitor and the blank fluorescence intensity, respectively. Each compound was assayed in 3 replicates each, each experiment being independently repeated three times. Selecting five to six concentrations of the compound, measuring the enzyme inhibition rate, performing linear regression by using the negative logarithm of the molar concentration of the compound and the enzyme inhibition rate, and obtaining the molar concentration when the 50% inhibition rate is obtained as the IC of the compound₅₀. The determination result shows that the 3-hydroxy chalcone Mannich base compound (I) disclosed in the embodiment of the invention has obvious inhibition effect on MAO-B, and IC of the compound₅₀The particle size is 0.5 to 15.0 mu M; IC for MAO-A inhibition₅₀Are all higher than 50.0 mu M, which indicates that the compound disclosed by the invention has selective inhibition effect on MAO-B. Experiments also find that in the chemical structural general formula of the 3-hydroxychalcone Mannich base compound (I), when R is₁、R₂And R₃While representing H, compounds having the same definition of other substituents as the general formula of the chemical structure, IC for MAO-B inhibition₅₀Are all higher than 80.0 mu M; 3-hydroxybenzaldehyde Mannich bases (2) IC for MAO-B inhibition₅₀Are all higher than 80.0 mu M; in addition, 3-OH-position OH and 4-CH position in the structural general formula of the 3-hydroxychalcone Mannich base compound (I)₂NR₄R₅After the substitution positions are interchanged, the obtained corresponding compounds have obviously reduced MAO-B inhibitory activity, and the corresponding IC₅₀Ratio of values (IC of Compounds after interchange)₅₀Value/corresponding Compound (I) IC₅₀Value) is at least greater than 3.0.

(5) Influence of 3-Hydroxychalcone Mannich base Compounds (I) on mouse memory acquisition disorder caused by scopolamine (examples 1-3)

SPF grade ICR male mice, 25-30g, randomly divided into: normal group, model group, positive control group, test drug high-low dose group (15.0 mg/kg, 2.5 mg/kg), each group of 10 animals. The tested medicine is given by one-time intragastric administration, the solvent of 0.5 percent CMC-Na is given to the blank group and the model group, and the administration volumes are both 0.1ml/10 g; injecting normal saline into abdominal cavity of normal group mice 45 min after administration, and injecting scopolamine (5 mg/kg) into other groups of animals, wherein the administration volume is 0.1ml/10 g; after 30 min of molding, the mice were placed in the non-electrostimulated Y maze for behavioral testing. During testing, a mouse is placed at the tail end of one arm, the mouse freely passes through the maze for 8 min, the times of entering each arm and the alternation times are recorded, and the alternation rate is calculated according to the following formula: alternation rate% = [ number of alternations/(total number of entries-2) ] × 100, results are expressed as mean ± standard deviation, and differences between groups were analyzed by one-way variance. The test result shows that under the experimental condition, the tested 3-hydroxychalcone Mannich base compound has a dose-dependent improvement effect on mouse acquired memory disorder caused by scopolamine, and has statistical difference (p < 0.01) compared with a model group, and the activity of the compound is remarkably higher than that of a clinical drug rivastigmine (p < 0.01) under the same molar concentration.

Detailed Description

The present invention will be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be understood by those skilled in the art that various changes and modifications may be made to the invention without departing from the spirit and scope of the invention.

EXAMPLE 13 general procedure for the preparation of Hydroxychalcone Mannich bases (I)

Adding 2.0 mmol of corresponding acetophenone compounds (1), 3.0 mmol of corresponding 3-hydroxybenzaldehyde Mannich base compounds (2) and 30 ml of ethanol into a reaction bottle, stirring uniformly, dropwise adding 12.0 mmol of 30% KOH aqueous solution, and stirring at room temperature for reaction for 3.0-40.0 hours (tracking the reaction process by TLC); after the reaction is finished, cooling to room temperature, adjusting the pH of the reaction solution to strong acidity by using a 10% hydrochloric acid aqueous solution, and dissolving the reaction solution by using saturated sodium bicarbonate waterAdjusting the pH of the reaction solution to be alkalescent, distilling off ethanol under reduced pressure, adding 80 mL of deionized water into the residual solution, extracting with 240 mL of dichloromethane for three times, combining organic layers, washing with saturated sodium chloride aqueous solution, drying with anhydrous sodium sulfate, filtering, distilling off the solvent under reduced pressure, and purifying the residue by column chromatography (eluent: dichloromethane: acetone =10:1 v/v) to obtain the corresponding 3-hydroxychalcone Mannich base compound (I), wherein the yield is 48.0-75.6%, and the chemical structures are all obtained by purifying the residue by column chromatography¹H-NMR、¹³C-NMR and ESI-MS confirmation; the purities of the obtained target substances are more than 97.0 percent through HPLC. The target prepared by the method has the following structure:

；

of partial compounds¹The H-NMR data are as follows:

¹H NMR (CDCl₃): 7.77 (d, J = 15.6 Hz, 1H), 7.68 (d, J = 8.0 Hz, 1H), 7.62 (s, 1H), 7.53 (d, J = 15.6 Hz, 1H), 7.17 (s, 1H), 7.06-7.00 (m, 2H), 6.93 (d, J = 8.0 Hz, 1H), 3.98 (s, 6H), 3.70 (s, 2H), 2.37 (s, 6H); ¹³C NMR (CDCl₃):188.4, 158.3, 153.1, 149.1, 143.8, 135.6, 131.2, 128.8, 124.4, 122.9, 121.3, 120.0, 114.7, 110.6, 109.9, 62.3, 56.0, 55.9, 44.3；

¹H NMR (CDCl₃): 7.73 (d, J = 15.6 Hz, 1H), 7.68 (d, J = 8.4 Hz, 1H), 7.62 (s, 1H), 7.52 (d, J = 15.6 Hz, 1H), 7.17 (s, 1H), 7.04 (s, 2H), 6.93 (d, 1H), 3.97 (s, 6H), 3.84 (s, 2H), 2.69 (q, J = 7.6 Hz, 4H), 1.15 (t, J = 7.6 Hz, 6H); ¹³C NMR (CDCl₃): 188.4, 158.5, 153.0, 149.0, 143.9, 135.4, 131.2, 128.7, 124.7, 122.8, 121.1, 119.9, 114.7, 110.6, 109.9, 56.5, 55.9, 55.8, 46.3, 10.9；

¹H NMR (CDCl₃): 7.73 (d, J = 15.6 Hz, 1H), 7.68 (d, J = 8.4 Hz, 1H) 7.61 (s, 1H), 7.52 (d, J = 15.6 Hz, 1H), 7.20 (s, 1H), 7.02 (s, 2H), 6.93 (d, J = 8.0 Hz, 1H), 3.97 (s, 6H), 3.79 (s, 2H), 3.16 (brs, 4H), 1.71 (brs, 4H), 1.54 (brs, 2H); ¹³C NMR (CDCl₃): 188.5, 158.2, 153.1, 149.0, 143.7, 135.7, 131.2, 129.3, 123.6, 122.9, 121.3, 120.0, 114.8, 110.5, 109.8, 61.1, 56.0, 55.9, 53.6, 25.3, 23.5；

¹H NMR (CDCl₃): 7.74 (d, J = 15.6 Hz, 1H), 7.68 (d, J = 7.6 Hz, 1H), 7.62 (s, 1H), 7.51 (d, J = 15.6 Hz, 1H), 7.36-7.30 (m, 5H), 7.15 (s, 1H), 7.04 (s, 2H), 6.93 (d, J = 8.0 Hz, 1H), 3.97 (s, 6H), 3.81 (s, 2H), 3.67 (s, 2H), 2.62 (q, J = 7.2 Hz, 2H), 1.16 (t, J = 7.2 Hz, 3H); ¹³C NMR (CDCl₃): 188.5, 158.2, 153.1, 149.1, 143.8, 136.5, 135.6, 131.3, 129.4, 129.0, 128.6, 127.6, 124.7, 122.9, 121.3, 120.2, 114.7, 110.6, 109.9, 57.5, 56.6, 56.0, 55.9, 46.6, 11.0；

¹H NMR (CDCl₃): 7.72 (d, J = 15.6 Hz, 1H), 7.64-7.61 (m, 2H), 7.50 (d, J = 15.6 Hz, 1H), 7.14 (s, 1H), 7.03-6.98 (m, 3H), 3.97 (s, 3H), 3.83 (s, 2H),2.67 (q, J = 7.2 Hz, 4H), 1.14 (t, J = 7.2 Hz, 6H)；

¹H NMR (CDCl₃): 7.73 (d, J = 15.6 Hz, 1H), 7.65-7.62 (m, 2H),7.50 (d, J = 15.6 Hz, 1H), 7.13 (s, 1H), 7.04-6.98 (m, 3H), 3.98 (s, 3H), 3.70 (s, 2H), 2.53 (brs, 4H), 1.67-1.64 (m, 4H), 1.51 (brs, 2H)；

¹H NMR (CDCl₃): 7.74 (d, J = 16.0 Hz, 1H), 7.59 (d, J = 8.0 Hz,1H), 7.54 (s, 1H), 7.49 (d, J = 16.0 Hz, 1H), 7.41 (t, J =8.0 Hz, 1H), 7.14-7.12 (m, 2H), 7.05-7.00 (m, 2H), 3.89(s, 3H), 3.81 (s, 2H), 2.65 (q, J = 7.2Hz, 4H), 1.13 (t, J = 7.2 Hz, 6H)；

¹H NMR (CDCl₃): 7.74 (d, J = 15.6 Hz, 1H), 7.59 (d, J = 7.6 Hz, 1H), 7.54-7.7.53 (m, 1H), 7.47 (d, J = 15.6 Hz, 1H), 7.41 (t, J = 8.0 Hz, 1H), 7.14-7.12 (m, 2H), 7.05-6.99 (m, 2H), 3.89 (s, 3H), 3.72 (s, 2H), 2.54 (brs, 4H),1.68-1.65 (m, 4H), 1.51 (brs, 2H)；

¹H NMR (CDCl₃): 8.03 (d, J = 8.4 Hz, 2H), 7.72 (d, J = 15.6 Hz, 1H), 7.50 (d, J = 15.6 Hz, 1H), 7.16 (s , 1H), 7.04-6.92 (m, 4H), 3.89 (s, 3H), 3.84 (s, 2H), 2.69 (q, J = 7.2 Hz, 4H), 1.16 (t, J = 7.2 Hz, 6H)。

EXAMPLE 23 preparation of hydroxy chalcone Mannich bases Compounds (I) by salt formation with an acid

Adding 2.0 mmol of 3-hydroxychalcone Mannich base compound (I) obtained in the embodiment 1 and 50 ml of acetone into a reaction bottle, uniformly stirring, adding 8.0 mmol of corresponding acid, heating, refluxing, stirring, reacting for 20 minutes, cooling to room temperature after the reaction is finished, and evaporating the solvent under reduced pressure to obtain the salt of the 3-hydroxychalcone Mannich base compound (I), wherein the chemical structure of the salt is obtained by¹H NMR and ESI-MS.

Claims (7)

1. A3-hydroxy chalcone Mannich base compound or a pharmaceutically acceptable salt thereof is characterized in that the chemical structure general formula of the compound is shown as (I):

in the formula: r₁、R₂And R₃Each independently represents H, dimethylamino, tetrahydropyrrolyl and piperidinyl, except that R₁、R₂And R₃Does not simultaneously represent H; r₁、R₂、R₃At any possible position on the corresponding phenyl ring; r₄And R₅Each independently represents methyl, ethyl, benzyl, substituted benzyl; NR (nitrogen to noise ratio)₄R₅Also tetrahydropyrrolyl, morpholinyl, piperidinyl, piperazinyl, 4-methylpiperazinyl, 4-benzylpiperazinyl, 4- ((methoxy) benzyl) piperazinyl; the "substituted benzyl" refers to a benzyl group substituted on the phenyl ring with 1 to 4 groups selected from the group consisting of: F. cl, Br, I, C_1-4Alkyl radical, C_1-4Alkoxy, trifluoromethyl, trifluoromethoxy, dimethylamino, these substituents being in any possible position on the phenyl ring of the benzyl group.

2. The 3-hydroxychalcone mannich base compound or the pharmaceutically acceptable salt thereof as claimed in claim 1, wherein the pharmaceutically acceptable salt is the 3-hydroxychalcone mannich base compound mixed with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid, sulfamic acid, C_1-6Aliphatic carboxylic acid, trifluoroacetic acid, stearic acid, pamoic acid, oxalic acid, benzoic acid, phenylacetic acid, salicylic acid, maleic acid, fumaric acid, succinic acid, tartaric acid, citric acid, malic acid, lactic acid, hydroxymaleic acid, pyruvic acid, glutamic acid, ascorbic acid, lipoic acid, C_1-6Salts of alkylsulfonic acids, camphorsulfonic acid, naphthalenesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid or 1, 4-butanedisulfonic acid.

3. A process for the preparation of 3-hydroxychalcone mannich base compounds or pharmaceutically acceptable salts thereof as claimed in any one of claims 1 to 2, characterized in that said compounds are prepared by:

in the formula: r₁～R₅The definition of (A) is the same as the chemical structural general formula of the 3-hydroxy chalcone Mannich base compound (I);

taking corresponding substituted acetophenone compound (1) and 3-hydroxybenzaldehyde Mannich base compound (2) as initial raw materials, and directly condensing under the conditions of solvent and alkali to obtain corresponding 3-hydroxychalcone Mannich base compound (I).

4. A process for the preparation of 3-hydroxychalcone mannich bases or their pharmaceutically acceptable salts as claimed in claim 3, characterized in that the base used in the reaction is: alkali metal hydroxide, alkaline earth metal hydroxide, alkali metal carbonate, alkaline earth metal carbonate, alkali metal bicarbonate, alkaline earth metal bicarbonate, C_1-8Alkali metal salts of alcohols, triethylamine, tributylamine, trioctylamine, pyridine,N-methylmorpholine,N-methylpiperidine, triethylenediamine, or tetrabutylammonium hydroxide; the solvent used in the reaction is: c_1-8Fatty alcohol, diethyl ether, tetrahydrofuran, 2-methyltetrahydrofuran,N,N-dimethylformamide, dimethyl sulfoxide, dichloromethane, chloroform, 1, 4-dioxane, benzene, toluene or acetonitrile.

5. The process for producing a 3-hydroxychalcone Mannich base compound or a pharmaceutically acceptable salt thereof according to claim 3, wherein the substituted acetophenone compound (1): 3-hydroxybenzaldehyde mannich base compounds (2): the molar charge ratio of alkali is 1.0: 1.0-3.0: 1.0 to 20.0; the reaction temperature is 0-150 ℃; the reaction time is 1-120 hours.

6. A pharmaceutical composition comprising a 3-hydroxychalcone mannich base compound as claimed in any one of claims 1 to 2 or a pharmaceutically acceptable salt thereof together with one or more pharmaceutically acceptable carriers or excipients.

7. Use of a 3-hydroxychalcone mannich base compound as claimed in any one of claims 1 to 2 or a pharmaceutically acceptable salt thereof for the preparation of a medicament for the treatment and/or prevention of neurological related disorders: vascular dementia, Alzheimer's disease, Parkinson's disease, Huntington's disease, HIV-related dementia, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma, ischemic stroke, hemorrhagic stroke, and nerve damage due to brain trauma.