CN109730311A - A kind of hoveniae semoveniae semen composite enzyme, preparation method and application - Google Patents
A kind of hoveniae semoveniae semen composite enzyme, preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of hoveniae semoveniae semen composite enzyme, preparation method and application, dry hoveniae semoveniae semen powder is taken, mulberries powder, sponge gourd, T.lobayense Heim powder, kudzu-vine root powder, dendrobium officinale powder is added fermentor, adds white sugar, honey, water is made into fermenation raw liquid；Four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum Freeze-dry Powder of Probioctics are taken to cultivate in the incubator respectively；It takes MRS solid medium tablets to be placed in shaking table, cultivates, the strain liquid 1:1:2:1 mixing by volume of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum obtains fermentation seed liquid；37.5 DEG C of constant temperature fermentations in fermenation raw liquid are added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%, are fermented 1~2 month；Filtering fermentation liquor, through high-temperature sterilization, except after alcohol natural hoveniae semoveniae semen composite enzyme.Realize natural resources makes full use of and integrates conversion, improves added value of product, also protects environment.
Description
Technical field
The present invention relates to a kind of foods processing technique more particularly to a kind of hoveniae semoveniae semen composite enzyme, preparation method and
Using.
Background technique
With the increase of operating pressure, modern stayed up late for work, it is more and more to treat the phenomenon that drinking with courtesy, is easy to damage liver
Kidney reduces immunity, accelerates aging.And the nutritional ingredients such as enzyme rich in, vitamin and mineral in ferment, have resist it is tired
Labor, removes the functions such as free radical at anti-aging.Ferment product is numerous at present, but is that fruit is got by spontaneous fermentation mostly, contains
Sugar amount is excessively high, and fermentation process is not easy to control, is easy to produce acid production alcohol, influences human health.
Ferment product is that fruit is got by spontaneous fermentation mostly at present, and sugar content is excessively high, and fermentation process is not easily-controllable
System is easy to produce acid production alcohol, influences human health.Beneficial microbe is not added, functional component is less, and effect is not significant, and
Fermentation residue abandons mostly not to be had to, and utilization efficiency is low, not only wastes natural resources, but also be unfavorable for environmental protection.Ferment product
It is made into liquid mostly, it is inconvenient to carry.
Summary of the invention
Technical problem to be solved by the present invention lies in: value-added content of product how is improved, integration of drinking and medicinal herbs is realized, provides
A kind of hoveniae semoveniae semen composite enzyme, preparation method and application.
The present invention is to solve above-mentioned technical problem, a kind of hoveniae semoveniae semen composite enzyme of the invention by the following technical programs
Preparation method, comprising the following steps:
(1) sampling mixing:
It is crushed after fresh hoveniae semoveniae semen is dried, mulberries dry grinding is broken to break into powder, fresh sponge gourd chopping, fresh T.lobayense Heim
It is crushed after freeze-drying；
(2) preparation of fermenation raw liquid:
Take 20~80 parts of dry hoveniae semoveniae semen powder, 10~50 parts of mulberries powder, 10~50 parts of sponge gourd, T.lobayense Heim powder 10~50
Part, 10~40 parts of kudzu-vine root powder, 10~40 parts of addition fermentors of dendrobium officinale powder add 50~200 parts of white sugar, honey 5~20
Part, 150~300 parts of water, it is made into fermenation raw liquid；
(3) preparation of fermentation seed liquid:
Take four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum one rings of Freeze-dry Powder of Probioctics in plate respectively
Upper scribing line, is cultivated in the incubator；One ring of the pure bacterium colony access for taking MRS solid medium tablets to be grown is equipped with seed culture fluid
Triangular flask in, be placed in shaking table, cultivate, saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum strain liquid press volume
It is mixed than 1:1:2:1, obtains fermentation seed liquid；
(4) it ferments:
37.5 DEG C of constant temperature fermentations in fermenation raw liquid, fermentation 1~2 is added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%
A month；
(5) prepared by composite enzyme:
By after fermentation filtering fermentation liquor, through high-temperature sterilization, except after alcohol natural hoveniae semoveniae semen composite enzyme.
As one of preferred embodiment of the invention, in the step (2), 20 parts of hoveniae semoveniae semen powder, 10 parts of mulberries powder, sponge gourd 10
Part, 10 parts of T.lobayense Heim powder, 10 parts of kudzu-vine root powder, 10 parts of addition fermentors of dendrobium officinale powder add 50 parts of white sugar, and 5 parts of honey, water
150 parts, it is made into fermenation raw liquid.
As one of preferred embodiment of the invention, in the step (2), 80 parts of hoveniae semoveniae semen powder, 50 parts of mulberries powder, sponge gourd 50
Part, 50 parts of T.lobayense Heim powder, 40 parts of kudzu-vine root powder, 40 parts of addition fermentors of dendrobium officinale powder add 200 parts of white sugar, and 20 parts of honey,
300 parts of water, it is made into fermenation raw liquid.
As one of preferred embodiment of the invention, in the step (2), 50 parts of hoveniae semoveniae semen powder, 30 parts of mulberries powder, sponge gourd 30
Part, 30 parts of T.lobayense Heim powder, 25 parts of kudzu-vine root powder, 25 parts of addition fermentors of dendrobium officinale powder add 140 parts of white sugar, and 14 parts of honey,
210 parts of water, it is made into fermenation raw liquid.
Composite enzyme made from a kind of preparation method of the hoveniae semoveniae semen composite enzyme, polysaccharide contains in the composite enzyme
It is 23.17~42.65mg/mL that amount, which is 30.15~56.38mg/mL, procyanidin content, total saponin content is 6.68~
14.36mg/mL, Vitamin C content are 7.6~9.8mg/mL.
Application of the composite enzyme in food preparation a kind of described in.
The composite enzyme is used to prepare pudding, congeals agent according to enzyme stoste, honey, citric acid, edible gelatin: fresh
The weight ratio 50:1:0.5:1:10 of milk is mixed with.
The composite enzyme is used to prepare pressed candy, specific as follows:
Composite enzyme stoste is spray-dried first, hoveniae semoveniae semen composite ferment powder is made, then according to the compound ferment of hoveniae semoveniae semen
Plain powder, isomaltoketose, crystallite calcium lactate weight ratio be 4:1:1 mixing, hoveniae semoveniae semen composite enzyme pressed candy is made in tabletting.
A kind of preparation method of hoveniae semoveniae semen composite enzyme filter residue obtained that ferments, the fermentation filter residue is used to resist
The preparation of whitening mask mud is aoxidized to smash remove bulky grain therein again, by weight after fermentation filter residue sterilizes in autoclave
Meter, addition bentonite 10~15%, Tween 80 1~5%, hyaluronic acid 1~5%, glycerol 2~6%, rosemary essence 0.1~
0.2%, uniform stirring to pureed prepares anti-oxidant whitening mask mud.
A kind of preparation method of hoveniae semoveniae semen composite enzyme filter residue obtained that ferments, the fermentation filter residue is used to resist
Aoxidize whitening mask mud preparation, fermentation filter residue sterilize in autoclave after, dry arrive it is half-dried, smash remove big therein again
Grain, in addition plain chocolate is stirred evenly with Self- raising flour, according to mass ratio, ferment filter residue: milk: low muscle face=8:3:7 takes egg
It is dismissed with shell egg, according to weight ratio fermentation filter residue: milk: Self- raising flour: white sugar is added in white sugar=8:3:7:3, beats to moist hair
It steeps, the egg liquid after dismissing foaming is added in batter in three times to stir evenly, and it is then placed in 180 DEG C of ovens and is baked 10 minutes,
Up to hoveniae semoveniae semen ferment cake.
Hoveniae semoveniae semen is commonly called as honey raisin tree also known as papaw, is a kind of natural plant raw material of integration of drinking and medicinal herbs.Modern medicine study
Show product chess piece containing the ingredients such as glucose, fructose, peroxidase, flavonoids, triterpenes, can remove and generate in vivo after drinking
Excessive free radicals hinder the formation of lipid peroxide, mitigate damage of the ethyl alcohol to hepatic tissue, avoid alcoholism.So edible
Hoveniae semoveniae semen can alleviate drunk symptom with relieving alcoholism, and kidney is protected in liver protection, adjusts immune.
In mulberries containing a large amount of activated protein, vitamin, amino acid, carrotene, resveratrol, anthocyanidin etc. at
Part, can antioxidant and anti-aging, nourishing yin and nourishing blood, improving eyesight protect liver, reducing blood lipid is hypoglycemic, enhancing immune function；
Sponge gourd is a kind of common vegetables, containing a large amount of sponge gourd saponin(e, has and adjusts immune, spot-removing beautifying face, whitening shield
Skin and other effects.
T.lobayense Heim, also known as Tricholoma Giganteum Massee, big spoken parts in traditional operas mushroom, Tricholoma lobayense are a kind of natural health edible mushrooms of preciousness,
T.lobayense Heim polysaccharide antioxidant activity with higher, it is suitable with VC, and activity of fighting against senium is significant.
Pueraria lobata, micro-pungent sweet in flavor, inducing diaphoresis are brought down a fever, relieving alcoholism, are adjusted function of human body, are built up health.There is expelling pathogenic factors from muscles and skin to bring down a fever, promoting eruption,
It promotes the production of body fluid to quench thirst, the function of Shengyang Zhixie.It is usually used in exterior syndrome fever, stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst, and heat is rushed down
Hot dysentery, splenasthenic diarrhea.Pueraria lobata contains daidzin, it can decompose acetaldehyde toxicity, liver protecting.
Dendrobium candidum, it is sweet in flavor, slightly cold, enter stomach, kidney channel, reinforcing stomach reg fluid, nourishing Yin and clearing heat.Contain dendrobium polysaccharide, dendrobium polysaccharide
A variety of medicinal ingredients such as alkali and 20 kinds of amino acid of needed by human body etc. have the beneficial stomach that promotes the production of body fluid, anti-aging, anti-oxidant and drop blood
The multiple efficacies such as sugar.
The present invention for the first time using a kind of integration of drinking and medicinal herbs natural plant raw material-hoveniae semoveniae semen as fermentation major ingredient, joint mulberries,
The natural raw-food material such as sponge gourd, T.lobayense Heim, pueraria lobata, dendrobium candidum, by adding four kinds of compound probiotics (saccharomycete, youth
Bifidobacterium, lactic acid bacteria, bifidobacterium longum) it ferments, it controls fermentation temperature and fermentation time, optimization for fermentation technology passes through
Biofermentation and bioconversion obtain then to measurable functional component progress functional evaluation is wherein stablized by techniques such as tablettings
To natural hoveniae semoveniae semen composite enzyme series of products, not only nutritive value is high for this series of products, also contains a large amount of functional component, such as
The various mixing enzymes such as polysaccharide, anthocyanin, saponins, flavonoids, various vitamins, SOD enzyme, lipase, have various function
Effect, as protect liver protects kidney, blood-enriching face-nourishing, beauty and skin care, promotion digestion and absorption, removing free radical, anti-oxidant antitumor, hypoglycemic drop
Blood lipid adjusts immune etc., is the functional product of health cares a series of, and the product of pressed candy fruit is easy to carry, can be with
Dietary supplementation at any time.Series of products are suitble to most of crowds, are especially suitable for the sub-health population often stayed up late, treat with courtesy and be addicted to drink.
And this product makes full use of various natural materials, and the filter residue after fermentation is comprehensively utilized and converted, and eats dry squeezing only, saves
Material and the energy protect environment.
It includes that medicine food is same that the present invention has the advantage that the present invention uses various natural raw-food materials compared with prior art
The raw-food material in source is inoculated with four kinds of compound probiotics and ferments, and controls zymotechnique and fermentation time, enzyme stoste are made into pressure
Piece candy and pudding, it is easy to carry as instant food, it can eat at any time；Ferment slag is made into facial mask mud and cake, realizes
Natural resources make full use of and integrate conversion, improve added value of product, also protect environment.
Detailed description of the invention
Fig. 1 is glucose content standard curve；
Fig. 2 is procyanidin content standard curve；
Fig. 3 is total saponin content standard curve；
Fig. 4 is Vitamin C content standard curve.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of preparation method of hoveniae semoveniae semen composite enzyme, specifically includes the following steps:
(1) 90 mesh are crushed to after drying fresh hoveniae semoveniae semen, mulberries dry grinding is broken to break into powder, and fresh sponge gourd shreds, newly
It is crushed after fresh T.lobayense Heim freeze-drying.Take 20 parts of dry hoveniae semoveniae semen powder, 10 parts of mulberries powder, 10 parts of sponge gourd, 10 parts of T.lobayense Heim powder, Pueraria lobota
10 parts of root powder, 10 parts of addition fermentors of dendrobium officinale powder add 50 parts of white sugar, 5 parts of honey, 150 parts of water, are made into proferment
Liquid；
(2) four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum one rings of Freeze-dry Powder of Probioctics is taken to exist respectively
Flat lining out is cultivated 24 hours for 30 DEG C in the incubator；One ring of the pure bacterium colony access for taking MRS solid medium tablets to be grown
In triangular flask equipped with 300ml seed culture fluid, it is placed in shaking table, condition of culture: revolving speed 100rpm, 37 DEG C of temperature, constant temperature incubation
18~24 hours, the strain liquid 1:1:2:1 mixing by volume of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum,
Obtain fermentation seed liquid；
(3) 37.5 DEG C of constant temperature fermentations in fermenation raw liquid are added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%, fermentation is just
Phase pays attention to Time deflation, ferments 1~2 month, and detect pH value；
(4) filtering with microporous membrane by the fermentation liquid aperture after fermentation for 0.5 μm, sterilized pot high-temperature sterilization (120
DEG C, 1h), natural hoveniae semoveniae semen composite enzyme is obtained after removing alcohol；
(5) it is detected and contains polysaccharide 30.15mg, procyanidine 23.17mg in every 1ml composite enzyme, total saposins 6.68mg,
Vitamin C 7.6mg.
Specific assay method is as follows for the present embodiment:
(1) measurement of the polysaccharide content
Method: phenol-sulfuric acid process
It takes sample a little, phenol solution is added, the concentrated sulfuric acid is added after mixing, boiling water bath is carried out after mixing again, after cooling
Its absorbance is surveyed under 485nm wavelength, acquired results substitute into standard curve, acquire Thick many candies content in sample, as shown in Figure 1.
(2) measurement of procyanidin content
Method: hydrochloric acid-n-butanol-ammonium ferric sulfate method
Sample is diluted, the hydrochloric acid solution of hydrochloric acid butanol solution and ammonium ferric sulfate is sequentially added, through boiling water bath heating and
Ice-water bath is cooling, its absorbance is surveyed under 546nm wavelength, and acquired results substitute into standard curve, acquire sample procyanidins and contain
Amount, as shown in Figure 2.
(3) measurement of total saponin content
Method: vanillin method
Take sample a little, after concentration vacuum drying obtain solid dry powder, successively plus 70% ethanol solution dissolution, filter into
In 10mL measuring bottle and with 70% ethyl alcohol constant volume, precision is drawn 1mL and is placed in 50mL tool plug test tube, cold after 100 DEG C of water-baths volatilize
But, it is added the 5% vanillic aldehyde glacial acetic acid solution 0.2mL that newly prepares, perchloric acid solution 0.8mL, 60 DEG C of heating water bath 15min take
Out, cooling with ice water immediately, then accurate addition glacial acetic acid 5mL, absorbance value is measured at wavelength 550nm, acquired results substitute into
Standard curve acquires total saponin content in sample, as shown in Figure 3.
(4) measurement of Vitamin C content
Method: Vitamin C acid system
It takes sample a little, after NaOH solution alkali process is added, using distilled water as blank, its suction is measured at 243nm wavelength
Luminosity, acquired results substitute into standard curve, acquire ascorbic content in sample, as shown in Figure 4.
Embodiment 2
A kind of preparation method of hoveniae semoveniae semen composite enzyme, specifically includes the following steps:
(1) 90 mesh are crushed to after drying fresh hoveniae semoveniae semen, mulberries dry grinding is broken to break into powder, and fresh sponge gourd shreds, newly
It is crushed after fresh T.lobayense Heim freeze-drying.Take 80 parts of dry hoveniae semoveniae semen powder, 50 parts of mulberries powder, 50 parts of sponge gourd, 50 parts of T.lobayense Heim powder, Pueraria lobota
40 parts of root powder, 40 parts of addition fermentors of dendrobium officinale powder add 200 parts of white sugar, 20 parts of honey, 300 parts of water, are made into proferment
Liquid；
(2) four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum one rings of Freeze-dry Powder of Probioctics is taken to exist respectively
Flat lining out is cultivated 24 hours for 30 DEG C in the incubator；One ring of the pure bacterium colony access for taking MRS solid medium tablets to be grown
In triangular flask equipped with 300ml seed culture fluid, it is placed in shaking table, condition of culture: revolving speed 100rpm, 37 DEG C of temperature, constant temperature incubation
18~24 hours, the strain liquid 1:1:2:1 mixing by volume of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum,
Obtain fermentation seed liquid；
(3) 37.5 DEG C of constant temperature fermentations in fermenation raw liquid are added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%, fermentation is just
Phase pays attention to Time deflation, ferments 1~2 month, and detect pH value；
(4) filtering with microporous membrane by the fermentation liquid aperture after fermentation for 0.5 μm, sterilized pot high-temperature sterilization (120
DEG C, 1h), natural hoveniae semoveniae semen composite enzyme is obtained after removing alcohol；
(5) contain polysaccharide 56.38mg, procyanidine 42.65mg, total saposins in every 1ml composite enzyme through detecting
14.36mg vitamin C 9.8mg.
Other embodiments and embodiment 1 are identical.
Embodiment 3
A kind of preparation method of hoveniae semoveniae semen composite enzyme, specifically includes the following steps:
(1) 90 mesh are crushed to after drying fresh hoveniae semoveniae semen, mulberries dry grinding is broken to break into powder, and fresh sponge gourd shreds, newly
It is crushed after fresh T.lobayense Heim freeze-drying.Take 50 parts of dry hoveniae semoveniae semen powder, 30 parts of mulberries powder, 30 parts of sponge gourd, 30 parts of T.lobayense Heim powder, Pueraria lobota
25 parts of root powder, 25 parts of addition fermentors of dendrobium officinale powder add 140 parts of white sugar, 14 parts of honey, 210 parts of water, are made into proferment
Liquid.
(2) four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum one rings of Freeze-dry Powder of Probioctics is taken to exist respectively
Flat lining out is cultivated 24 hours for 30 DEG C in the incubator；One ring of the pure bacterium colony access for taking MRS solid medium tablets to be grown
In triangular flask equipped with 300ml seed culture fluid, it is placed in shaking table, condition of culture: revolving speed 100rpm, 37 DEG C of temperature, constant temperature incubation
18~24 hours, the strain liquid 1:1:2:1 mixing by volume of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum,
Obtain fermentation seed liquid；
(3) 37.5 DEG C of constant temperature fermentations in fermenation raw liquid are added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%, fermentation is just
Phase pays attention to Time deflation, ferments 1~2 month, and detect pH value；
(4) filtering with microporous membrane by the fermentation liquid aperture after fermentation for 0.5 μm, sterilized pot high-temperature sterilization (120
DEG C, 1h), natural hoveniae semoveniae semen composite enzyme is obtained after removing alcohol；
(5) contain polysaccharide 43.26mg, procyanidine 34.57mg, total saposins in every 1ml composite enzyme through detecting
32.91mg vitamin C 8.7mg.
Other embodiments and embodiment 1 are identical.
Embodiment 4
A kind of preparation method of hoveniae semoveniae semen composite enzyme pressed candy, specifically includes the following steps:
1 gained composite enzyme stoste of embodiment is spray-dried, 120 DEG C of inlet air temperature <, 50 DEG C of leaving air temp <, object
Hoveniae semoveniae semen composite ferment powder is made in 55~60 DEG C of vapo(u)rizing temperature of material, and isomaltoketose, crystallite calcium lactate etc. are added wherein and matches
The weight ratio of material, hoveniae semoveniae semen composite ferment powder and isomaltoketose, crystallite calcium lactate is 3:1:1, and it is compound that hoveniae semoveniae semen is made in tabletting
Ferment pressed candy.
2 gained composite enzyme stoste of embodiment is spray-dried, 120 DEG C of inlet air temperature <, 50 DEG C of leaving air temp <, object
Hoveniae semoveniae semen composite ferment powder is made in 55~60 DEG C of vapo(u)rizing temperature of material, and isomaltoketose, crystallite calcium lactate etc. are added wherein and matches
The weight ratio of material, hoveniae semoveniae semen composite ferment powder and isomaltoketose, crystallite calcium lactate is 6:2:1, and it is compound that hoveniae semoveniae semen is made in tabletting
Ferment pressed candy.
3 gained composite enzyme stoste of embodiment is spray-dried, 120 DEG C of inlet air temperature <, 50 DEG C of leaving air temp <, object
Hoveniae semoveniae semen composite ferment powder is made in 55~60 DEG C of vapo(u)rizing temperature of material, and isomaltoketose, crystallite calcium lactate etc. are added wherein and matches
The weight ratio of material, hoveniae semoveniae semen composite ferment powder and isomaltoketose, crystallite calcium lactate is 4:1:1, and it is compound that hoveniae semoveniae semen is made in tabletting
Ferment pressed candy.
Embodiment 5
A kind of preparation method of hoveniae semoveniae semen composite enzyme pudding, specifically includes the following steps:
It will be separately added into citric acid, honey, edible gelatin in 1,2,3 gained composite enzyme stoste of embodiment and congeal agent, new
Fresh milk etc. prepares hoveniae semoveniae semen composite enzyme pudding, by weight ratio, enzyme stoste: honey, citric acid, edible gelatin are congealed
Agent: fresh milk: 50:1:0.5:1:10.The pudding made is sparkling and crystal-clear clear, bright and lustrous, sour-sweet pleasant.
Embodiment 6
A kind of preparation method of hoveniae semoveniae semen composite enzyme facial mask mud, specifically includes the following steps:
The filter residue of the resulting composite enzyme of embodiment 1,2,3 is sterilized in autoclave after (120 DEG C, 1h), regulation machine is used
It smashes again and removes bulky grain therein, bentonite 10~15%, Tween 80 1~5%, hyaluronic acid 1~5%, glycerol is added
2~6%, rosemary essence 0.1~0.2%, uniform stirring to pureed prepares anti-oxidant whitening mask mud, this facial mask mud silty
Exquisiteness, it is clear bright, cool on the skin is applied, skin is more compact after cleaning within 5~10 minutes, and whitening effect is obvious.
Embodiment 7
A kind of preparation method of hoveniae semoveniae semen composite enzyme cake, specific method the following steps are included:
The filter residue of the resulting composite enzyme of embodiment 1,2,3 is sterilized in autoclave after (120 DEG C, 1h), is slightly dried to half
It is dry, smashed again with regulation machine and remove bulky grain therein, in addition plain chocolate and Self- raising flour stir evenly (according to quality meter,
Fermentation residue: milk: low muscle face=8:3:7), it takes egg to dismiss with shell egg, the appropriate (fermentation residue: milk: low muscle face of white sugar is added
Powder: white sugar=8:3:7:3), it beats to moist foaming.Egg liquid after foaming will be dismissed is added in batter stirs evenly in three times.So
After be put into 180 DEG C of ovens and be baked 10 minutes to get hoveniae semoveniae semen ferment cake.Cake is bright and lustrous, soft agreeable to the taste, fragrant and sweet suitable
People.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of preparation method of hoveniae semoveniae semen composite enzyme, which comprises the following steps:
(1) sampling mixing:
It is crushed after fresh hoveniae semoveniae semen is dried, mulberries dry grinding is broken to break into powder, fresh sponge gourd chopping, fresh T.lobayense Heim freeze-drying
After crush；
(2) preparation of fermenation raw liquid:
Take 20~80 parts of dry hoveniae semoveniae semen powder, 10~50 parts of mulberries powder, 10~50 parts of sponge gourd, 10~50 parts of T.lobayense Heim powder, Pueraria lobota
10~40 parts of root powder, 10~40 parts of addition fermentors of dendrobium officinale powder add 50~200 parts of white sugar, and 5~20 parts of honey, water
150~300 parts, it is made into fermenation raw liquid；
(3) preparation of fermentation seed liquid:
Four kinds of saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum one rings of Freeze-dry Powder of Probioctics are taken to draw on plate respectively
Line is cultivated in the incubator；One ring of the pure bacterium colony access for taking MRS solid medium tablets to be grown is equipped with the three of seed culture fluid
In the bottle of angle, be placed in shaking table, cultivate, saccharomycete, bifidobacterium adolescentis, lactic acid bacteria, bifidobacterium longum strain liquid by volume 1:
1:2:1 mixing, obtains fermentation seed liquid；
(4) it ferments:
37.5 DEG C of constant temperature fermentations in fermenation raw liquid are added in the fermenting microbe liquid for accounting for fermenation raw liquid volume 3%, are fermented 1~2 month；
(5) prepared by composite enzyme:
By after fermentation filtering fermentation liquor, through high-temperature sterilization, except after alcohol natural hoveniae semoveniae semen composite enzyme.
2. a kind of preparation method of hoveniae semoveniae semen composite enzyme according to claim 1, which is characterized in that in the step (2),
20 parts of hoveniae semoveniae semen powder, 10 parts of mulberries powder, 10 parts of sponge gourd, 10 parts of T.lobayense Heim powder, 10 parts of kudzu-vine root powder, 10 parts of addition hairs of dendrobium officinale powder
Fermentation tank adds 50 parts of white sugar, 5 parts of honey, 150 parts of water, is made into fermenation raw liquid.
3. a kind of preparation method of hoveniae semoveniae semen composite enzyme according to claim 1, which is characterized in that in the step (2),
80 parts of hoveniae semoveniae semen powder, 50 parts of mulberries powder, 50 parts of sponge gourd, 50 parts of T.lobayense Heim powder, 40 parts of kudzu-vine root powder, 40 parts of addition hairs of dendrobium officinale powder
Fermentation tank adds 200 parts of white sugar, 20 parts of honey, 300 parts of water, is made into fermenation raw liquid.
4. a kind of preparation method of hoveniae semoveniae semen composite enzyme according to claim 1, which is characterized in that in the step (2),
50 parts of hoveniae semoveniae semen powder, 30 parts of mulberries powder, 30 parts of sponge gourd, 30 parts of T.lobayense Heim powder, 25 parts of kudzu-vine root powder, 25 parts of addition hairs of dendrobium officinale powder
Fermentation tank adds 140 parts of white sugar, 14 parts of honey, 210 parts of water, is made into fermenation raw liquid.
5. composite enzyme made from a kind of preparation method such as the described in any item hoveniae semoveniae semen composite enzymes of Claims 1 to 4,
Be characterized in that, in the composite enzyme polyoses content be 30.15~56.38mg/mL, procyanidin content be 23.17~
42.65mg/mL, total saponin content are 6.68~14.36mg/mL, and Vitamin C content is 7.6~9.8mg/mL.
6. a kind of application of composite enzyme as claimed in claim 5 in food preparation.
7. application according to claim 6, which is characterized in that the composite enzyme is used to prepare pudding, according to zymogen
Liquid, honey, citric acid, edible gelatin congeal agent: the weight ratio 50:1:0.5:1:10 of fresh milk is mixed with.
8. application according to claim 6, which is characterized in that the composite enzyme is used to prepare pressed candy, specifically such as
Under:
Composite enzyme stoste is spray-dried first, be made hoveniae semoveniae semen composite ferment powder, then according to hoveniae semoveniae semen composite ferment powder,
Isomaltoketose, crystallite calcium lactate weight ratio be 4:1:1 mixing, hoveniae semoveniae semen composite enzyme pressed candy is made in tabletting.
9. ferment filter residue made from a kind of preparation method such as the described in any item hoveniae semoveniae semen composite enzymes of Claims 1 to 4,
It is characterized in that, the fermentation filter residue is used for the preparation of anti-oxidant whitening mask mud, after fermentation filter residue sterilizes in autoclave, then
Secondary smash removes bulky grain therein, by weight, addition bentonite 10~15%, Tween 80 1~5%, and hyaluronic acid 1~
5%, glycerol 2~6%, rosemary essence 0.1~0.2%, uniform stirring to pureed prepares anti-oxidant whitening mask mud.
10. ferment filter residue made from a kind of preparation method such as the described in any item hoveniae semoveniae semen composite enzymes of Claims 1 to 4,
It is characterized in that, the fermentation filter residue is used for the preparation of anti-oxidant whitening mask mud, after fermentation filter residue sterilizes in autoclave,
It dries half-dried, smashes remove bulky grain therein again, in addition plain chocolate is stirred evenly with Self- raising flour, according to mass ratio, hair
Ferment filter residue: milk: low muscle face=8:3:7 takes egg to dismiss with shell egg, according to weight ratio fermentation filter residue: milk: Self- raising flour:
White sugar=8:3:7:3 addition white sugar, beats to moist foaming, the egg liquid after dismissing foaming is added in batter in three times and stirs
It is even, it is then placed in 180 DEG C of ovens and is baked 10 minutes to get hoveniae semoveniae semen ferment cake.
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