CN109730154A - A kind of probiotics donkey milk piece and preparation method thereof - Google Patents
A kind of probiotics donkey milk piece and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of probiotics donkey milk piece and preparation method thereof, the probiotics donkey milk piece includes the raw material of following parts by weight: 40-50 parts of donkey milk powder；2-5 parts of oligofructose；6-8 parts of isomaltose；Probiotics 0.6 × 105‑1×105cfu/mL；6-8 parts of dextrin；2-4 parts of mannitol；2-3 parts of citric acid；0.05-0.2 parts of magnesium stearate；0.45-0.8 parts of talcum powder.The present invention also provides preparation methods.Probiotics donkey milk piece of the invention comprehensively utilizes donkey milk, and probiotics donkey milk piece had not only had the function of the nutritive value of milk but also had the special physiological of probiotics, integrates nutrition and health care, also has strengthen immunity, anti-oxidant antifatigue, antitumor and bacteriostasis.Probiotics donkey milk piece of the invention not only improves the content of the probiotics in probiotics donkey milk piece and the content of essential amino acid by fermenting three times, is conducive to absorption of human body；But also various flavor substances are generated, improve the mouthfeel of milk tablet.
Description
Technical field
The invention belongs to field of health care food.More particularly, to a kind of donkey milk piece of strengthen immunity and its preparation side
Method.
Background technique
Donkey milk has high lactalbumin, unsaturated fatty acid height and Vitamin C content high, low fat, low cholesterol, low junket
Albumen；Selenium-rich, be rich in lysozyme, calcium phosphorus ration rationally, amino acid pattern nutrition closest to breast milk natural milk.In various albumen
In matter, the nutritive value highest of lactalbumin, referred to as immune protein content compares cow's milk in " king of albumen " donkey milk lactalbumin
It is about high by 43%, it is about higher by 15% than breast milk.Donkey milk nutrition content and composition are closest with human milk, and because of its unique immune tune
Section, anti-oxidant antifatigue, antitumor and bacteriostasis are by nutrition circle and the world of medicine's most attention.
Prebiotics can be used as a kind of meal supplement regulator, optionally increase the quantity of probiotics, optimize flora
Carry out the health of balanced human, some oligosaccharide in prebiotics also have the function of dietary fiber, can increase the retentiveness of stool
And capacity plays the role of " enteral street cleaner " to be easy to be discharged, and plays bidirectional modulation to constipation, diarrhea, also adsorbable intestines
Anion in road, bile acid and blood lipid and cholesterol is effectively reduced.Main functional oligose is oligomeric maltose, oligomeric half
Lactose, oligofructose, isomaltose, milk ketose, xylo-oligosaccharide, stachyose, raffinose, soyabean oligosaccharides, synanthrin etc..Synbiotic
Also known as Synbiotics refers to the combination preparation of probiotics and prebiotics.It can not only play the physiological bacterial activity of probiotics, but also
The quantity for optionally quickling increase this bacterium keeps probiotic action more significant lasting, jointly to anti-disease, safeguards body
Microecological balance.
The present invention applies synbiotic in donkey milk powder, while providing nutrition to people, and has strengthen immunity, adjusts
The effect of intestinal flora is saved, there is huge development potentiality.
Summary of the invention
In order to solve the above technical problems, the first purpose of this invention is to provide a kind of probiotics donkey of strengthen immunity
Milk tablet；The probiotics donkey milk piece not only has the mouthfeel of fragrance, but also there are also strengthen immunities, the work of adjusting intestinal flora
With.
Second object of the present invention is to provide a kind of preparation method of probiotics donkey milk piece.
A kind of probiotics donkey milk piece, the raw material including following parts by weight:
40-50 parts of donkey milk powder；
2-5 parts of oligofructose；
6-8 parts of isomaltose；
Probiotics 0.6 × 105-1×10 5cfu/g；
6-8 parts of dextrin；
2-4 parts of mannitol；
2-3 parts of citric acid；
0.05-0.2 parts of magnesium stearate；
0.45-0.8 parts of talcum powder.
Further, the probiotics donkey milk piece, the raw material including following parts by weight:
50 parts of donkey milk powder；
4 parts of oligofructose；
5 parts of isomaltose；
Probiotics 0.8 × 105cfu/g；
6 parts of dextrin；
3 parts of mannitol；
2 parts of citric acid；
0.1 part of magnesium stearate；
0.5 part of talcum powder.
Further, the probiotics is bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus and lactobacillus acidophilus.
A kind of preparation method of probiotics donkey milk piece, includes the following steps:
1) probiotics is seeded to and is added in liquid-rich culture medium, and the Anaerobic culturel 9-15h at 35-45 DEG C, it then will training
The bacterium solution for supporting base is centrifugated at 1-10 DEG C, removes supernatant, collects probio thalline；
2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 1-3h at 35-45 DEG C, is obtained
Prebiotic bacteria suspension；
3) the donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crushed
80 meshes obtain donkey milk piece coarse powder；
4) the donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, is then dried, and obtains intermediate
Grain；
5) magnesium stearate, talcum powder and intermediate particle are mixed, then tabletting, crosses 80 meshes, obtains probiotic milk
Piece；
6) product inspection: detection live probiotics bacterium number is 5.9 × 106cfu/g。
Further, the pressure of the tabletting is 36MPa, and the working environment of tabletting is 20 DEG C, and relative humidity 5% is made
Diameter 2.5cm, the milk tablet of thick 1cm；This is because milk powder is easy the moisture absorption, temperature is high, and humidity conference progressive die is unsmooth to cause sticking existing
As not easily molded, pressure will cause greatly sliver or tablet breakage, and unilateral recess is rough, and compacting efficiency reduces.
Further, the drying is the lower natural drying of humidity 84% or drying at 16 DEG C of temperature, the time 33 days.
Further, described plus liquid-rich culture medium refers to based on the fluid whey culture medium of mass concentration 6%-12%
Culture medium, then adding yeast extract to its mass concentration is 1%-10%, addition corn pulp to its volumetric concentration is 1%-
5%.
Further, a kind of preparation method of probiotics donkey milk piece, includes the following steps:
1) probiotics is seeded to and is added in liquid-rich culture medium, and the Anaerobic culturel 9-15h at 35-45 DEG C, it then will training
The bacterium solution for supporting base is centrifugated at 1-10 DEG C, removes supernatant, collects probio thalline；
2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 1-3h at 35-45 DEG C, is obtained
Prebiotic bacteria suspension；
3) the donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crushed
80 meshes obtain donkey milk piece coarse powder；
4) after being sufficiently mixed the donkey milk piece coarse powder and prebiotic bacteria suspension uniformly, 0.9% renin is added, stirring is equal
Whey is stood and removed after even, and when the ziega pH after standing is 4.4, ziega is fitted into the row of suspension in the nylon net bag of 80 mesh
Except whey, grumeleuse is obtained；
5) when the grumeleuse has whey precipitation, 0.05% flavor yeast bacterium Ku Deaziwei Pichia pastoris N-X is added
Pichia kudriavzevii N-X is fermented again, and fermentation time 3 days, 17 DEG C of temperature；Be conducive to the required of intestinal absorption
Amino acid, obtained ziega is dry, and granulation obtains intermediate particle.
6) magnesium stearate, talcum powder and intermediate particle are mixed, then tabletting, crosses 80 meshes, obtains probiotic milk
Piece；
7) product inspection: detection live probiotics bacterium number is 5.9 × 106cfu/g。
Flavor yeast bacterium Ku Deaziwei Pichia pastoris N-X Pichia added by present invention third time fermentation
Kudriavzevii N-X is preserved in the bacterial strain of China typical culture collection center with deposit number CCTCC NO:M2017759.
The bacterial strain is to extract to obtain from milk pimple.The milk pimple is Xinjiang Minority traditional zymotic dairy produce.
Probiotics donkey milk piece of the invention not only comprehensively utilizes donkey milk by unique ferment three times, but also joined benefit
Raw bacterium；It can achieve and eat immediately, easy to carry, mouthfeel is aromatic, has antitumor, antibacterial, strengthen immunity and adjusts enteron aisle
The effect of flora.
The probiotics of the invention mass propagation after first time fermented and cultured, prolific probiotics inoculate to liquid
It carries out cultivating for second in body donkey milk culture medium, cultivates probiotics under the living environment for being more nearly finished product, through liquid donkey
The survival rate of probiotics in probiotics donkey milk piece is improved after the culture screening of newborn culture medium.
Third time fermentation of the invention can efficiently be decomposed lactalbumin in donkey milk, to improve probiotics donkey
The content of the essential amino acid for being conducive to intestinal absorption in milk tablet, is more advantageous to absorption of human body, and the stomach for improving human body is inhaled
Receive function.
In addition, the present invention uses preservation saccharomycete Ku Deaziwei Pichia pastoris N-X (Pichia in third time fermentation
Kudriavzevii N-X), used Ku Deaziwei Pichia pastoris N-X (Pichia kudriavzevii N-X) is simultaneously
With stronger beta galactosidase, protease, esterase and lipase vigor, lactose, fat and protein are in yeast secretion
Beta galactosidase, protease, lipase etc. under the action of resolve into glucose, amino acid and fatty acid, in various enzymes
Under effect, through glucose fermentation, amino acid metabolism and fatty acid metabolism, generate various flavor substances, as alcohols, aldehydes, esters,
The flavor substances such as acids, ketone improve the mouthfeel of milk tablet.
Beneficial effects of the present invention are as follows:
1, probiotics donkey milk piece of the invention has probiotics to the healthcare function of human body, can generate and disappear in human body intestinal canal
Change acid, and vitamin can be generated, including pantothenic acid, niacin, B1, B2,85, B6 and vitamin K etc.；Short chain friend can be generated simultaneously
Fat acid, antioxidant, amino acid etc. play an important role to bone growth and health of heart；Certain diseases can be prevented and treated,
Such as bowel syndrome, respiratory tract infection, allergy, halitosis, gastric ulcer, diarrhoea in humans and constipation and other diseases.
2, probiotics donkey milk piece of the invention to donkey milk comprehensively utilize, probiotics donkey milk piece not only there is the nutritive value of milk but also
Have the function of the special physiological of probiotics, integrate nutrition and health care, also there is strengthen immunity, it is anti-oxidant antifatigue, anti-
Tumour and bacteriostasis.
3, probiotics donkey milk piece of the invention overcomes the storage of liquid probiotics dairy products difficulty, and the shelf-life is short, and effect is difficult to send out
The disadvantages of waving.
Specific embodiment
In order to illustrate more clearly of the present invention, below with reference to preferred embodiment, the present invention is described further.Ability
Field technique personnel should be appreciated that following specifically described content is illustrative and be not restrictive, this should not be limited with this
The protection scope of invention.
Embodiment 1
A kind of probiotics donkey milk piece, the raw material including following parts by weight: 50 parts of donkey milk powder；2 parts of oligofructose；Different malt
5 parts of sugar；Probiotics 0.8 × 105cfu/mL；6 parts of dextrin；4 parts of mannitol；2 parts of citric acid；0.05 part of magnesium stearate；Talcum powder
0.8 part.
A kind of preparation method of probiotics donkey milk piece, includes the following steps:
Step 1) by probiotics be seeded to plus liquid-rich culture medium in, and the Anaerobic culturel 10h at 40 DEG C then will training
The bacterium solution for supporting base is centrifugated at 10 DEG C, removes supernatant, collects probio thalline；Liquid-rich culture medium is added to refer to quality
The fluid whey culture medium of concentration 12% is basic culture medium, then adding yeast extract to its mass concentration is 5%, and addition is beautiful
Rice & peanut milk to its volumetric concentration is 1%.Probiotics is bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus and lactobacillus acidophilus.
Step 2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 2h at 37 DEG C, it obtains prebiotic
Bacteria suspension；
Step 3) donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crush
80 meshes are crossed, donkey milk piece coarse powder is obtained；
Step 4) donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, then at 16 DEG C of temperature, humidity
84% lower natural drying is dried 33 days, and intermediate particle is obtained；
Step 5) magnesium stearate, talcum powder and intermediate particle are mixed, then tabletting, 80 meshes are crossed, probiotic milk is obtained
Piece；The pressure of tabletting is 36MPa, and the working environment of tabletting is 20 DEG C, and diameter 2.5cm is made in relative humidity 5%, thick 1cm's
Probiotic milk tablets；
Step 6) product inspection: detection live probiotics bacterium number is 5.0 × 106cfu/g。
It is stored 6 months under donkey milk piece room temperature of the present invention.
Probiotics donkey milk piece of the invention comprehensively utilizes donkey milk, and probiotics donkey milk piece had not only had the nutritive value of milk but also had
The special physiological function of profitable probliotics, integrates nutrition and health care, also has strengthen immunity, anti-oxidant antifatigue, anti-swollen
Tumor and bacteriostasis.
Functional oligose in probiotics donkey milk piece of the present invention can provide nutrition not only for probiotics, selectively increase
Add the quantity of probiotics, optimization probiotics carrys out the health of balanced human.In addition, oligosaccharide also has the function of dietary fiber, it can
Increase the retentiveness defecated and capacity and play the role of " enteral street cleaner " to be easy to be discharged, constipation, diarrhea is played double
To adjusting, anion in also adsorbable enteron aisle, bile acid and blood lipid and cholesterol is effectively reduced.
Probiotics donkey milk piece of the invention has probiotics to the healthcare function of human body, and digestion can be generated in human body intestinal canal
Acid, and vitamin can be generated, including pantothenic acid, niacin, B1, B2,85, B6 and vitamin K etc.；Short chain friend fat can be generated simultaneously
Acid, antioxidant, amino acid etc. play an important role to bone growth and health of heart；Certain diseases can be prevented and treated, such as
Bowel syndrome, respiratory tract infection, allergy, halitosis, gastric ulcer, diarrhoea in humans and constipation and other diseases.
The probiotics of the invention mass propagation after first time fermented and cultured, prolific probiotics inoculate to liquid
It carries out cultivating for second in body donkey milk culture medium, cultivates probiotics under the living environment for being more nearly finished product, through liquid donkey
The survival rate of probiotics in probiotics donkey milk piece is improved after the culture screening of newborn culture medium.
Embodiment 2
A kind of probiotics donkey milk piece, the raw material including following parts by weight: 50 parts of donkey milk powder；4 parts of oligofructose；Different malt
5 parts of sugar；Probiotics 0.8 × 105cfu/mL；6 parts of dextrin；3 parts of mannitol；
2 parts of citric acid；0.1 part of magnesium stearate；0.5 part of talcum powder.
A kind of preparation method of probiotics donkey milk piece, includes the following steps:
Step 1) by probiotics be seeded to plus liquid-rich culture medium in, and the Anaerobic culturel 12h at 37 DEG C then will training
The bacterium solution for supporting base is centrifugated at 10 DEG C, removes supernatant, collects probio thalline；Liquid-rich culture medium is added to refer to quality
The fluid whey culture medium of concentration 6% is basic culture medium, then adding yeast extract to its mass concentration is 1%, and addition is beautiful
Rice & peanut milk to its volumetric concentration is 5%.Probiotics is bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus and lactobacillus acidophilus.
Step 2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 3h at 37 DEG C, it obtains prebiotic
Bacteria suspension；
Step 3) donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crush
80 meshes are crossed, donkey milk piece coarse powder is obtained；
Step 4) donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, then at 16 DEG C of temperature, humidity
84% lower natural drying is dried 33 days, and intermediate particle is obtained；
Step 5) magnesium stearate, talcum powder and intermediate particle are mixed, then tabletting, 80 meshes are crossed, probiotic milk is obtained
Piece；The pressure of tabletting is 36MPa, and the working environment of tabletting is 20 DEG C, and diameter 2.5cm is made in relative humidity 5%, thick 1cm's
Probiotic milk tablets；
Step 6) product inspection: detection live probiotics bacterium number is 5.9 × 106cfu/g。
Probiotics donkey milk piece of the invention comprehensively utilizes donkey milk, and probiotics donkey milk piece had not only had the nutritive value of milk but also had
The special physiological function of profitable probliotics, integrates nutrition and health care, also has strengthen immunity, anti-oxidant antifatigue, anti-swollen
Tumor and bacteriostasis.Probiotics donkey milk piece of the invention overcomes the storage of liquid probiotics dairy products difficulty, and the shelf-life is short, and effect is difficult to
The disadvantages of performance.
Functional oligose in probiotics donkey milk piece of the present invention can provide nutrition not only for probiotics, selectively increase
Add the quantity of probiotics, optimization probiotics carrys out the health of balanced human.In addition, oligosaccharide also has the function of dietary fiber, it can
Increase the retentiveness defecated and capacity and play the role of " enteral street cleaner " to be easy to be discharged, constipation, diarrhea is played double
To adjusting, anion in also adsorbable enteron aisle, bile acid and blood lipid and cholesterol is effectively reduced.
Probiotics donkey milk piece of the invention has probiotics to the healthcare function of human body, and digestion can be generated in human body intestinal canal
Acid, and vitamin can be generated, including pantothenic acid, niacin, B1, B2,85, B6 and vitamin K etc.；Short chain friend fat can be generated simultaneously
Acid, antioxidant, amino acid etc. play an important role to bone growth and health of heart；Certain diseases can be prevented and treated, such as
Bowel syndrome, respiratory tract infection, allergy, halitosis, gastric ulcer, diarrhoea in humans and constipation and other diseases.
The probiotics of the invention mass propagation after first time fermented and cultured, prolific probiotics inoculate to liquid
It carries out cultivating for second in body donkey milk culture medium, cultivates probiotics under the living environment for being more nearly finished product, through liquid donkey
The survival rate of probiotics in probiotics donkey milk piece is improved after the culture screening of newborn culture medium.
Embodiment 3
A kind of probiotics donkey milk piece, the raw material including following parts by weight: 50 parts of donkey milk powder；4 parts of oligofructose；Different malt
5 parts of sugar；Probiotics 0.8 × 105cfu/g；6 parts of dextrin；3 parts of mannitol；
2 parts of citric acid；0.1 part of magnesium stearate；0.5 part of talcum powder.
A kind of preparation method of probiotics donkey milk piece, includes the following steps:
Step 1) by probiotics be seeded to plus liquid-rich culture medium in, and the Anaerobic culturel 12h at 37 DEG C then will training
The bacterium solution for supporting base is centrifugated at 10 DEG C, removes supernatant, collects probio thalline；Liquid-rich culture medium is added to refer to quality
The fluid whey culture medium of concentration 6% is basic culture medium, then adding yeast extract to its mass concentration is 1%, and addition is beautiful
Rice & peanut milk to its volumetric concentration is 5%.Probiotics is bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus and lactobacillus acidophilus.
Step 2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 3h at 37 DEG C, it obtains prebiotic
Bacteria suspension；
Step 3) donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crush
80 meshes are crossed, donkey milk piece coarse powder is obtained；
Step 4) donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, then at 16 DEG C of temperature, humidity
84% lower natural drying is dried 33 days, and intermediate particle is obtained；
Step 5) magnesium stearate, talcum powder and intermediate particle are mixed, 0.9% renin is added, it is quiet after mixing evenly
Whey is set and removed, when the ziega pH after standing is 4.4, ziega is fitted into suspension in the nylon net bag of 80 mesh and excludes cream
Clearly, grumeleuse is obtained；
Step 6) in step 5) grumeleuse when having whey precipitation, add 0.05% flavor yeast bacterium Ku Deaziweibi
Red yeast N-X Pichia kudriavzevii N-X is fermented again, and fermentation time 3 days, 17 DEG C of temperature；It is solidifying by what is obtained
Newborn block is dry, obtains intermediate particle；Then tabletting crosses 80 meshes, obtains probiotic milk tablets；The pressure of tabletting is 36MPa, tabletting
Working environment be 20 DEG C, diameter 2.5cm, the probiotic milk tablets of thick 1cm are made in relative humidity 5%；
Step 7) product inspection: detection live probiotics bacterium number is 5.9 × 106cfu/g。
Third time fermentation of the invention can efficiently be decomposed lactalbumin in donkey milk, to improve probiotics donkey
The content of essential amino acid in milk tablet, is more advantageous to absorption of human body, improves the stomach absorption function of human body.
In addition, the present invention uses preservation saccharomycete Ku Deaziwei Pichia pastoris N-X (Pichia in third time fermentation
Kudriavzevii N-X), used Ku Deaziwei Pichia pastoris N-X (Pichia kudriavzevii N-X) is simultaneously
With stronger beta galactosidase, protease, esterase and lipase vigor, lactose, fat and protein are in yeast secretion
Beta galactosidase, protease, lipase etc. under the action of resolve into glucose, amino acid and fatty acid, in various enzymes
Under effect, through glucose fermentation, amino acid metabolism and fatty acid metabolism, generate various flavor substances, as alcohols, aldehydes, esters,
The flavor substances such as acids, ketone improve the mouthfeel of milk tablet.
Embodiment 4
A kind of probiotics donkey milk piece, the raw material including following parts by weight: 50 parts of donkey milk powder；5 parts of oligofructose；Different malt
6 parts of sugar；Probiotics 1 × 105cfu/g；6 parts of dextrin；3 parts of mannitol；
2 parts of citric acid；0.1 part of magnesium stearate；0.5 part of talcum powder.
A kind of preparation method of probiotics donkey milk piece, includes the following steps:
Step 1) by probiotics be seeded to plus liquid-rich culture medium in, and the Anaerobic culturel 12h at 37 DEG C then will training
The bacterium solution for supporting base is centrifugated at 10 DEG C, removes supernatant, collects probio thalline；Liquid-rich culture medium is added to refer to quality
The fluid whey culture medium of concentration 6% is basic culture medium, then adding yeast extract to its mass concentration is 1%, and addition is beautiful
Rice & peanut milk to its volumetric concentration is 5%.Probiotics is bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus and lactobacillus acidophilus.
Step 2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 3h at 37 DEG C, it obtains prebiotic
Bacteria suspension；
Step 3) donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crush
80 meshes are crossed, donkey milk piece coarse powder is obtained；
Step 4) donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, then at 16 DEG C of temperature, humidity
84% lower natural drying is dried 33 days, and intermediate particle is obtained；
Step 5) magnesium stearate, talcum powder and intermediate particle are mixed, 0.9% renin is added, it is quiet after mixing evenly
Whey is set and removed, when the ziega pH after standing is 4.4, ziega is fitted into suspension in the nylon net bag of 80 mesh and excludes cream
Clearly, grumeleuse is obtained；
Step 6) in step 5) grumeleuse when having whey precipitation, add 0.05% flavor yeast bacterium Ku Deaziweibi
Red yeast N-X Pichia kudriavzevii N-X is fermented again, and fermentation time 3 days, 17 DEG C of temperature；It is solidifying by what is obtained
Newborn block is dry, obtains intermediate particle；Then tabletting crosses 80 meshes, obtains probiotic milk tablets；The pressure of tabletting is 36MPa, tabletting
Working environment be 20 DEG C, diameter 2.5cm, the probiotic milk tablets of thick 1cm are made in relative humidity 5%；
Step 7) product inspection: detection live probiotics bacterium number is 4.8 × 106cfu/g。
Third time fermentation of the invention can efficiently be decomposed lactalbumin in donkey milk, to improve probiotics donkey
The content of essential amino acid in milk tablet, is more advantageous to absorption of human body, improves the stomach absorption function of human body.
In addition, the present invention uses preservation saccharomycete Ku Deaziwei Pichia pastoris N-X (Pichia in third time fermentation
Kudriavzevii N-X), used Ku Deaziwei Pichia pastoris N-X (Pichia kudriavzevii N-X) is simultaneously
With stronger beta galactosidase, protease, esterase and lipase vigor, lactose, fat and protein are in yeast secretion
Beta galactosidase, protease, lipase etc. under the action of resolve into glucose, amino acid and fatty acid, in various enzymes
Under effect, through glucose fermentation, amino acid metabolism and fatty acid metabolism, generate various flavor substances, as alcohols, aldehydes, esters,
The flavor substances such as acids, ketone improve the mouthfeel of milk tablet.
Embodiment 5
200 patients are chosen in the inferior health outpatient service of hospital and carry out test-meal investigation, and patient criteria is no organic disease, only
Body is in sub-health state, and hypoimmunity, the symptoms such as easy fatigue and weak, constipation occurs in patient.In 200 volunteer patients
Men and women's number is fifty-fifty, and the age is 30-55 years old, and average age is 45.5 years old.
It is required that investigation person takes 30 grams of the probiotics donkey milk piece of embodiment 3 daily, continuously take 60 days.Finding is aobvious
Show: the effect of continuously eating this probiotics donkey milk piece, capable of significantly reaching strengthen immunity.Specific manifestation are as follows: have rosy cheeks, energy
Plentifully, immunity enhancing, constipation improve.
1 donkey milk piece finding of table
| Project | Edible number | Improvement number | It is efficient |
| Face is dull | 200 | 156 | 78% |
| Physical fatigue | 200 | 180 | 90% |
| Hypoimmunity | 200 | 190 | 95% |
| Constipation | 200 | 188 | 94% |
As shown in Table 1, after by the edible for patients of experimental group donkey milk piece of the invention, the function of strengthen immunity can significantly be reached
Effect, be embodied in have rosy cheeks, be energetic, immunity enhancing, constipation improve.This is because the donkey in donkey milk piece of the present invention
Milk powder and probiotics synergistic effect, have the effect of benefiting qi and nourishing blood, strengthen immunity.
It can also be made other variations or changes in different ways on the basis of above description, it here can not be to all realities
The mode of applying is exhaustive, all to belong to obvious changes or variations that technical solution of the present invention is extended out still in this
The column of the protection scope of invention.
Claims (8)
1. a kind of probiotics donkey milk piece, which is characterized in that the raw material including following parts by weight:
40-50 parts of donkey milk powder；
2-5 parts of oligofructose；
6-8 parts of isomaltose；
Probiotics 0.6 × 105-1×105cfu/g；
6-8 parts of dextrin；
2-4 parts of mannitol；
2-3 parts of citric acid；
0.05-0.2 parts of magnesium stearate；
0.45-0.8 parts of talcum powder.
2. probiotics donkey milk piece according to claim 1, which is characterized in that the raw material including following parts by weight:
50 parts of donkey milk powder；
4 parts of oligofructose；
5 parts of isomaltose；
Probiotics 0.8 × 105cfu/g；
6 parts of dextrin；
3 parts of mannitol；
2 parts of citric acid；
0.1 part of magnesium stearate；
0.5 part of talcum powder.
3. probiotics donkey milk piece according to claim 2, which is characterized in that the probiotics is bifidobacterium lactis, cheese
Lactobacillus, Lactobacillus rhamnosus and lactobacillus acidophilus.
4. a kind of preparation method of the probiotics donkey milk piece as described in claims 1 to 3 is any, which is characterized in that including as follows
Step:
1) probiotics is seeded to and is added in liquid-rich culture medium, and the Anaerobic culturel 9-15h at 35-45 DEG C, then by culture medium
Bacterium solution be centrifugated at 1-10 DEG C, remove supernatant, collect probio thalline；
2) probio thalline is seeded in liquid donkey milk culture medium, and the Anaerobic culturel 1-3h at 35-45 DEG C, is obtained prebiotic
Bacteria suspension；
3) the donkey milk powder, oligofructose, isomaltose, dextrin, mannitol and citric acid are sufficiently mixed, and crushed 80 mesh
Sieve, obtains donkey milk piece coarse powder；
4) the donkey milk piece coarse powder and prebiotic bacteria suspension are sufficiently mixed uniformly；Granulation, then dries, obtains intermediate particle；
5) magnesium stearate, talcum powder and intermediate particle are mixed, then tabletting, crosses 80 meshes, obtains probiotic milk tablets；
6) product inspection: detection live probiotics bacterium number is 5.9 × 106cfu/。
5. the preparation method according to claim 4, which is characterized in that the pressure of the tabletting is 36MPa, the work of tabletting
Environment is 20 DEG C, and diameter 2.5cm, the probiotic milk tablets of thick 1cm are made in relative humidity 5%.
6. the preparation method according to claim 4, which is characterized in that the drying is under 16 DEG C of temperature, humidity 84%
Natural drying or drying, the time 33 days.
7. the preparation method according to claim 4, which is characterized in that described plus liquid-rich culture medium refers to mass concentration
The fluid whey culture medium of 6%-12% is basic culture medium, then adding yeast extract to its mass concentration is 1%-10%,
Adding corn pulp to its volumetric concentration is 1%-5%.
8. the preparation method according to claim 4, which is characterized in that further include following steps in step 4):
A) after being sufficiently mixed the donkey milk piece coarse powder and prebiotic bacteria suspension uniformly, 0.9% renin is added, after mixing evenly
Whey is stood and removed, when the ziega pH after standing is 4.4, ziega is fitted into suspension in the nylon net bag of 80 mesh and excludes cream
Clearly, grumeleuse is obtained；
B) when the grumeleuse of step a) has whey precipitation, 0.05% flavor yeast bacterium Ku Deaziwei Pichia pastoris N-X is added
Pichia kudriavzevii N-X is fermented again, and fermentation time 3 days, 17 DEG C of temperature, so that the lactalbumin in donkey milk
Resolve into the essential amino acid for being conducive to intestinal absorption；Obtained ziega is dry, obtain intermediate particle.
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| CN114794245A (en) * | 2022-05-13 | 2022-07-29 | 扬州市扬大康源乳业有限公司 | Probiotic milk tablet and preparation method thereof |
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Application publication date: 20190510 |