CN109700033B - Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof - Google Patents
Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof Download PDFInfo
- Publication number
- CN109700033B CN109700033B CN201910161548.7A CN201910161548A CN109700033B CN 109700033 B CN109700033 B CN 109700033B CN 201910161548 A CN201910161548 A CN 201910161548A CN 109700033 B CN109700033 B CN 109700033B
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- powder
- parts
- composition
- constipation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a probiotic composition for regulating intestinal tracts and preventing constipation, which takes lactobacillus plantarum powder and lactobacillus plantarum metabolite powder as active ingredients. The embodiment of the invention provides a probiotic composition for regulating intestinal tracts and preventing constipation, which is capable of effectively improving constipation diarrhea and a plurality of diseases caused by constipation and diarrhea of middle-aged and elderly people and improving the flora environment of the intestinal tracts.
Description
Technical Field
The invention relates to the technical field of microbial preparations, in particular to a probiotic composition for regulating intestinal tracts and preventing constipation and a preparation method thereof.
Background
The laxative health-care food in the market has more types, the preparation forms mainly comprise capsules, tea drinks, powder and oral liquid, the used raw materials mainly comprise senna leaves, rheum officinale, fructus cannabis, immature bitter orange and other materials with laxative effects, the materials have side effects on human bodies, the constipation problem can be solved only for a while, the constipation cannot be cured radically, and the research and development of products are not realized through the conditioning principle. The traditional Chinese medicines are used as main components in a large proportion, the health care effect is different, some have higher manufacturing cost, and some have more complex production process.
Probiotics are active microorganisms which play a role by improving the balance of host intestinal microbial flora, are important physiological bacteria in human intestinal tracts, and have important physiological effects of improving the intestinal flora structure, promoting the proliferation of beneficial bacteria in the intestinal tracts, inhibiting the growth of harmful bacteria, eliminating carcinogenic factors, improving the immunity of organisms, reducing cholesterol, relieving lactose intolerance and the like. However, the existing drugs or related probiotic products have no obvious effects on treating and preventing constipation, treating diarrhea and regulating intestinal tracts, and further improvement is urgently needed.
Disclosure of Invention
The embodiment of the invention aims to provide a probiotic composition for regulating intestinal tracts and preventing constipation and a preparation method thereof, and aims to overcome the defects that the existing product is poor in effect of treating diarrhea and constipation and complex in preparation process of the product.
In order to achieve the above objects, the present invention provides a probiotic composition for regulating intestinal tract and preventing constipation, which comprises lactobacillus plantarum powder and lactobacillus plantarum metabolite powder as active ingredients.
Preferably, the lactobacillus plantarum is preserved in the China center for type culture Collection with the preservation number: CCTCC NO: m2018042.
Preferably, the composition also comprises a fungus powder carrier, wherein the fungus powder carrier comprises microcrystalline cellulose, medicinal corn starch, inulin and magnesium stearate;
the composition comprises the following raw materials in parts by mass: 9-11 parts of lactobacillus plantarum powder, 4-6 parts of lactobacillus plantarum metabolite powder, 18-22 parts of microcrystalline cellulose, 80-120 parts of medicinal corn starch, 8-12 parts of inulin and 8-12 parts of magnesium stearate.
Preferably, the composition comprises the following raw materials in parts by mass: 9.5-10.5 parts of lactobacillus plantarum powder, 4.5-5.5 parts of lactobacillus plantarum metabolite powder, 19-21 parts of microcrystalline cellulose, 90-110 parts of medicinal corn starch, 9-11 parts of inulin and 9-11 parts of magnesium stearate.
The embodiment of the invention provides a method for preparing the composition, which comprises the steps of carrying out expanded strain culture, primary seed culture, seed tank culture and fermentation culture on lactobacillus plantarum to obtain lactobacillus plantarum zymocyte liquid;
centrifuging the lactobacillus plantarum zymocyte liquid to respectively collect lactobacillus plantarum bacterial sludge and centrifuged fermentation liquor, and respectively freezing, drying, crushing, concentrating, drying and crushing the lactobacillus plantarum bacterial sludge and the centrifuged fermentation liquor to obtain lactobacillus plantarum bacterial powder and lactobacillus plantarum metabolite powder;
and mixing the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder with a bacterium powder carrier to obtain the composition.
Preferably, the preparation method of the lactobacillus plantarum fungus powder specifically comprises the following steps:
and mixing the lactobacillus plantarum bacterium paste, sterile water, skimmed milk powder, fructo-oligosaccharide, glycerol, maltodextrin and isomaltooligosaccharide to obtain lactobacillus plantarum suspended bacterium liquid, and freeze-drying and crushing the suspended bacterium liquid to obtain lactobacillus plantarum bacterium powder.
Preferably, the suspension liquid is pre-frozen for 2 to 5 hours at the temperature of between 35 ℃ below zero and 30 ℃ below zero, and is subjected to vacuum freeze drying at the temperature of between 50 ℃ below zero and 40 ℃ below zero, and is crushed into lactobacillus plantarum powder with 100 meshes.
Preferably, the preparation method of the lactobacillus plantarum metabolite powder specifically comprises the following steps:
preferably, the centrifugal fermentation liquid is first subjected to low-temperature concentration to obtain a fermentation liquid concentrated paste with the water content of at most 20%, and the fermentation liquid concentrated paste and the medicinal corn starch are mixed in a ratio of 5: 1-4: 1 to obtain a mixture, then drying the mixture for 40 to 50 hours at the temperature of 55 to 60 ℃, and crushing the mixture to obtain the lactobacillus plantarum metabolite powder with the water content of at most 3 percent.
The composition prepared by the method disclosed by the invention is applied to preparing a product with at least one of the following functions:
1) regulating intestinal flora;
2) preventing constipation;
3) can be used for treating diarrhea.
The embodiment of the invention has the following advantages:
the embodiment of the invention provides a probiotic composition for regulating intestinal tracts and preventing constipation, wherein the contained lactobacillus plantarum powder can effectively improve constipation diarrhea and a plurality of diseases brought by the constipation diarrhea of middle-aged and elderly people and improve the flora environment of the intestinal tracts.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The embodiment of the invention provides a probiotic composition for regulating intestinal tracts and preventing constipation, which is mainly used for improving constipation diarrhea and various diseases caused by constipation of middle-aged and elderly people and improving the flora environment of the intestinal tracts.
The embodiment of the invention firstly obtains a new strain, the strain is separated from fresh bitter gourd epidermis, is obtained by primary screening and secondary screening, is identified as lactobacillus plantarum by 16S DNA, and the sequence of the strain is shown as SEQ ID NO. 1. And the strain is subjected to biological preservation by a patent program, and is preserved in China Center for Type Culture Collection (CCTCC) in 2018, 01 month and 17 days, wherein the preservation number is CCTCC NO: m2018042.
Example 1
One, the enlarged strain culture of lactobacillus plantarum powder
The strain of the embodiment of the invention adopts lactobacillus plantarum, and the equipment used in the production comprises the following components: a strain incubator, an ultra-clean workbench, a constant-temperature incubator, various test tubes, a triangular flask, a seeding tank, a fermentation tank, a vacuum freeze dryer, a three-dimensional stirrer, a low-temperature crusher and a vacuum packaging machine.
Strain culture, expanded culture of strain preserved at-80 deg.c, preparation of expanded strain culture medium: 20 g of glucose, 12 g of peptone, 6 g of beef extract powder, 2g of sodium acetate, 801 ml of tween, 0.5 g of urea, 1000 ml of tap water and natural pH value.
Adding glucose and other raw materials into 1000 ml of water in the formula, stirring for 30min to dissolve, then subpackaging in 18 x 180 test tubes, each test tube containing 10 ml, sealing with rubber plug kraft paper, sterilizing in a medical sterilizer at 0.1-0.12 MPa for 25min, and sterilizing the expanded strain culture medium.
Opening a freezing tube which is preserved at the temperature of minus 80 ℃ and is filled with lactobacillus plantarum in a super-clean workbench under the aseptic condition, melting at room temperature, then taking 1 count of the sterilized test tube culture medium, adding 1ml of the dissolved preservation bacteria liquid at the temperature of minus 80 ℃ into a test tube, sealing the tube opening by using a rubber plug and kraft paper, shaking up by hand, and placing into a constant temperature incubator for standing and culturing for 13 hours at the temperature of 37 ℃ to obtain the expanded strain culture solution.
Secondly, first-order seed culture of lactobacillus plantarum powder
The formula of the first-level seed culture medium is as follows: 26 g of glucose, 5 g of peptone, 4 g of beef extract powder, 3g of corn steep liquor, 2g of sodium acetate, 801 ml of tween and 1000 ml of tap water, and the pH value is natural.
Adding the above materials into tap water, stirring for 20min, and dissolving to obtain primary seed culture solution. Adding the stirred and dissolved first-stage seed culture solution into a triangular flask according to the using amount, then binding a bottle mouth with 6 layers of gauze and a layer of kraft paper, putting the bottle mouth into a medical sterilizer for sterilization, firstly opening an exhaust valve on the sterilizer before sterilization and closing when a small amount of steam is exhausted, opening the exhaust valve on the sterilizer when the pressure on the sterilizer reaches 0.05 MPa, exhausting for 6-8min, then closing the exhaust valve, continuing heating, starting timing when the steam pressure in the sterilizer reaches 0.1-0.12 MPa, finishing sterilization for 25min, leaving a heat source after sterilization, naturally cooling, opening the sterilizer when the pressure gauge on the sterilizer returns to zero, taking out the sterilized culture medium, putting the sterilized culture medium into an ultra-clean workbench for naturally cooling, transferring the cultured expanded strain solution into the sterilized culture solution in the triangular flask when the temperature is reduced to 37 ℃, the inoculation amount is 1 percent, namely 100 milliliters of first-stage seed culture solution, 1 milliliter of expanded strain culture solution is inoculated, after the inoculation is finished, the bottle mouth is sealed by using original gauze and kraft paper, the bottle mouth is slightly shaken by hands to uniformly mix the inoculated strain and the culture solution, and then the mixture is cultured in a thermostat for 13 hours at the temperature of 37 ℃, so that the first-stage seed culture solution is obtained.
Thirdly, the lactobacillus plantarum powder seeding tank culture
Sterilizing fermentation equipment, pipelines and a sterile filtration system, firstly opening valves of each inlet and outlet pipeline and a sterile air pipeline, introducing 0.12-0.14 MPa steam, communicating the steam with the pipeline valves and discharging a small amount of steam, introducing the steam for 40min, keeping the steam pressure in the pipelines at 0.12-0.14 MPa for 40min, and then closing valves of each inlet and outlet pipeline for later use.
Sterilizing empty seed tank and fermentation tank, closing valves, opening drain valve at bottom of the tank and drain valve in interlayer, opening direct steam valve, introducing 0.12-0.14 MPa steam into the tank, timing when the temperature in the tank reaches 121 deg.C, sterilizing for 40min, closing the drain valve and interlayer drain valve at bottom of the tank, and naturally cooling to 37 deg.C.
The formula of the seeding tank culture medium comprises, by weight, 10% of glucose, 1% of peptone, 0.5% of urea, 0.2% of dipotassium hydrogen phosphate, 2% of corn steep liquor, 0.2% of sodium acetate, 0.78% of tween 800.4, 2% of polydextrose, 0.1% of a polyether defoamer, 83.6% of tap water and natural pH value.
Firstly, putting tap water into a seeding tank sterilized in an empty tank, starting a stirrer, respectively adding raw materials required in a formula, then introducing steam for heating in continuous stirring, firstly, heating to 95 ℃ by using steam in an interlayer of the tank, then directly heating by using the steam, keeping the temperature in the tank for 30min when the temperature reaches 121 ℃ to achieve the sterilization effect, then closing the steam, starting cooling tap water passing through the interlayer of the tank, and keeping for later use when the temperature in the tank reaches 37 ℃, wherein the culture medium is called a seeding tank culture medium. Then, inoculating the cultured first-stage strain culture solution into a seed tank under aseptic conditions, wherein the inoculation amount is 1% of the culture medium in the tank respectively, namely 100kg of the culture medium in the seed tank is inoculated into 1kg of the first-stage strain culture solution respectively. Then, the inoculation cap on the tank is covered, and the stirring culture is started, wherein the culture conditions are as follows: the temperature is 37 ℃, the PH value is natural, the stirring speed is 80 r/min, the tank pressure is 0.05 MPa, the culture time can reach the requirement after 18 hours, if the tank pressure is reduced, sterile air can be introduced to maintain the tank pressure, and if the tank pressure is too high, the tank pressure can be adjusted by a gas release valve on the tank top, and the seed tank culture strains are obtained.
Fourth, fermentation culture of lactobacillus plantarum powder
The fermentation medium comprises the following components in percentage by weight: 10% of glucose, 2% of peptone, 3% of polydextrose, 12% of isomaltose hypgather, 2% of sodium acetate, 800.1% of tween, 5% of potato starch, 0.5% of conjugated linoleic acid, 0.3% of anhydrous calcium chloride, 0.5% of magnesium sulfate, 4% of corn steep liquor, 0.1% of polyether defoamer and 60.5% of tap water, wherein the total amount is 100 kg.
Firstly, the tap water used in the formula is put into a fermentation tank which is sterilized in an empty tank, a stirrer is started, the raw materials used in the formula are put into the fermentation tank, and then steam is introduced for heating. Firstly, heating to 95 ℃ by using interlayer steam, then heating by using direct steam, timing when the temperature in a tank reaches 121 ℃, keeping for 30min to achieve a sterilization effect, then cooling and cooling by using interlayer tap water, starting inoculating strains when the temperature in the tank is reduced to 37 ℃, firstly increasing the tank pressure of a seeding tank to 0.1 MPa and the tank pressure of a fermentation tank to 0.05 MPa before inoculation, then opening an inoculation pipeline valve, conveying the seeds cultured in the seeding tank to the fermentation tank in a pressure difference mode, and then closing the inoculation pipeline valve.
The inoculation amount is 3kg of strains cultured in a seeding tank inoculated to every 100kg of culture medium in a fermentation tank, the culture condition is that the fermentation temperature is 37 ℃, the stirring speed is 120 r/min, the fermentation time is 16 hours, the tank pressure is kept at 0.05 MPa, the pH value is natural, if the tank pressure is reduced, sterile air can be introduced to keep the tank pressure, and if the tank pressure is too high, the tank pressure can be adjusted by an exhaust valve on the top of the tank, so that the fermentation liquid is obtained.
Fifthly, freeze drying of lactobacillus plantarum
After fermentation, the fermentation liquid is pumped into a storage tank, and the selection of the centrifugal rotating speed is very important in order to ensure the integrity of thalli in the fermentation liquid. Starting a tubular centrifuge, adjusting the rotating speed to 12000 r/min, centrifuging at the feeding speed of 15kg of feed per min, collecting wet bacterial sludge after centrifugation is finished, freeze-drying the centrifugally collected wet bacterial sludge, carrying out low-temperature vacuum concentration on the centrifuged fermentation liquor, adding a carrier into the fermentation liquor, and compounding a finished product after vacuum drying.
1. Preparing suspension liquid, taking 1000 g of wet bacterial mud, adding 3000 ml of sterile distilled water, 300 g of skim milk powder, 200 g of fructo-oligosaccharide, 100ml of 98% glycerol, 50 g of maltodextrin and 300 g of isomaltooligosaccharide. Firstly, 1500 ml of water in the formula is heated to 50-55 ℃, maltodextrin is added under stirring, the remaining water in the formula is added after the maltodextrin is dissolved, and then other raw materials in the formula are added. Stirring thoroughly for 30min (stirring at 25-35 ℃). Obtaining a suspension liquid, and then putting the suspension liquid into a thermostatic chamber or a box with the temperature of 30 ℃ for thermostatic 30min for pre-freezing.
Pre-freezing before freeze drying, namely sub-packaging the suspension liquid with constant temperature for 30min into a freeze drying tray, then pre-freezing the suspension liquid in a pre-freezing chamber of a freeze dryer for 3 hours at the temperature of minus 30-35 ℃, and vacuumizing and drying after freezing. The vacuum drying conditions were: putting the pre-frozen material tray into a vacuum drying chamber to begin vacuum drying, wherein the drying conditions are as follows: the vacuum degree is 2-5 Pa, the temperature is-45 ℃, the temperature of the drying chamber is 28 ℃, and the drying time is 45 hours. And after freeze-drying, collecting freeze-dried materials, crushing the freeze-dried materials into bacterial powder with the fineness of 100 meshes by using an aseptic crusher, and sealing and packaging the bacterial powder for later use to obtain the lactobacillus plantarum bacterial powder.
2. Low-temperature concentration of centrifugal fermentation liquor: concentrating under vacuum degree of 0.06 MPa at 55-65 deg.C to obtain paste with solid content of 80% and water content of 20%, adding medicinal corn starch as carrier, mixing, and vacuum drying. Taking 1000 g of concentrated paste, adding 200 g of medicinal corn starch, uniformly mixing, and drying in a vacuum drying oven, wherein the drying conditions are as follows: drying at 55-60 deg.C under 0.06 MPa for 45 hr until the water content is 3%, pulverizing to 100 mesh powder, and sealing and packaging to obtain Lactobacillus plantarum metabolite powder.
The probiotic composition for regulating the intestinal tract and preventing constipation is prepared by the following steps: taking 5 g of lactobacillus plantarum powder of 100 meshes, 3g of lactobacillus plantarum metabolite powder, 10 g of microcrystalline cellulose, 20 g of medicinal corn starch, 5 g of inulin and 5 g of magnesium stearate, wherein the microcrystalline cellulose, the medicinal corn starch, the inulin and the magnesium stearate are carriers of the bacterium powder.
Mixing the above materials in solid stirring mixer, packaging under aseptic condition into capsule, and bottling or tabletting to obtain the final product.
Example 2
The probiotic composition for regulating the intestinal tract and preventing constipation is prepared by the following steps: 9 g of lactobacillus plantarum powder of 100 meshes, 6 g of lactobacillus plantarum metabolite powder, 22 g of microcrystalline cellulose, 120 g of medicinal corn starch, 12 g of inulin and 12 g of magnesium stearate are taken.
Wherein, the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder are both the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder prepared in the method of example 1.
Example 3
The probiotic composition for regulating the intestinal tract and preventing constipation is prepared by the following steps: 9 g of lactobacillus plantarum powder of 100 meshes, 4 g of lactobacillus plantarum metabolite powder, 22 g of microcrystalline cellulose, 120 g of medicinal corn starch, 8 g of inulin and 8 g of magnesium stearate are taken.
Wherein, the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder are both the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder prepared in the method of example 1.
Example 4
The probiotic composition for regulating the intestinal tract and preventing constipation is prepared by the following steps: taking 11 g of lactobacillus plantarum powder of 100 meshes, 6 g of lactobacillus plantarum metabolite powder, 18 g of microcrystalline cellulose, 80 g of medicinal corn starch, 8 g of inulin and 8 g of magnesium stearate.
Wherein, the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder are both the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder prepared in the method of example 1.
Example 5
The probiotic composition for regulating the intestinal tract and preventing constipation is compounded as follows: taking 11 g of lactobacillus plantarum powder of 100 meshes, 6 g of lactobacillus plantarum metabolite powder, 18 g of microcrystalline cellulose, 80 g of medicinal corn starch, 12 g of inulin and 12 g of magnesium stearate.
Wherein, the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder are both the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder prepared in the method of example 1.
Example 6 prevention of Constipation in mice induced by activated carbon with Lactobacillus plantarum powder
60 female C57B6/N mice weighing about 23g were selected and divided into 3 groups on average, namely a normal group, a constipation control group and a Lactobacillus plantarum powder treatment group. After adaptive feeding for 1 week, the mice in the normal group and the constipation control group normally take free diet and water for 2 weeks; in addition to the normal free intake of diet and drinking water during these 2 weeks, the lactobacillus plantarum powder-treated group mice were fed 0.2g of lactobacillus plantarum powder per day per one mouse, respectively (50 billion/CFU per gram of live lactobacillus plantarum). After 2 weeks, 2mL of activated carbon water (4 ℃ C., 10% by mass of activated carbon was added to an aqueous solution containing 10% gum arabic to prepare a suspension) was daily gavaged in 2 groups of mice except for the normal group of mice, and the group treated with lactobacillus plantarum powder was fed with lactobacillus plantarum powder for 3 days. All mice were measured daily at morning intervals for the duration of the experiment for body weight, dietary intake, water intake, fecal weight and fecal moisture. The weight of mice on days 1-17 was as follows:
TABLE 1
And (3) test results: the body weight change is an important index of constipation of mice, and as shown in table 1, the body weights of the mice in each group in the first 2 weeks all show a normal growth state, and no obvious difference exists among the groups; after constipation is induced by activated carbon, the weight of the normal group of mice which are not treated by the activated carbon continues to increase, and the weights of the rest groups of mice all decrease, wherein the weight of the constipation control group of mice decreases most, and the weight of the lactobacillus plantarum powder treated group of mice is closest to that of the normal group, which shows that the lactobacillus plantarum powder can inhibit the weight decrease of the mice caused by constipation.
Example 7
Table 2 shows the clinical effects of the probiotic compositions for regulating intestinal tract and preventing constipation in examples 1 to 5 on improving constipation and diarrhea
Table 2 application effects of the probiotic composition for regulating intestinal tract and preventing constipation according to the embodiments of the present invention on improvement of constipation and diarrhea in the elderly
TABLE 2
As can be seen from Table 2, 300 elderly men in the male group started to take the composition for 3 days, and 82 people of different ages who took the composition for 3 days felt constipation and diarrhea to be relieved; after the medicine is taken for 15 days, 143 people feel constipation and diarrhea are obviously improved; after the medicine is taken for 30 days, 232 people feel that defecation is smooth, and the body is relaxed; after the medicine is taken for 60 days, 288 people feel constipation, diarrhea is completely improved, defecation is normal, and the colitis of 122 people is improved; during this test period, 12 people who took Lactobacillus plantarum had no improvement in constipation and diarrhea.
The statistical effect starts when 300 elderly women in the group take the medicine for 3 days, and 77 people of different ages feel constipation and diarrhea after taking the medicine for 3 days; after the medicine is taken for 15 days, 135 people feel constipation and diarrhea are obviously improved; after the medicine is taken for 30 days, 229 people feel that the defecation is smooth and the body is relaxed; after the medicine is taken for 60 days, 291 people feel constipation and diarrhea are completely improved, defecation is normal, and the colitis of 109 people is improved; during this test period, 9 people who took lactobacillus plantarum had no improvement in constipation and diarrhea.
TABLE 3 application effects of probiotic compositions for regulating intestinal tract and preventing constipation according to the examples of the present invention on reduction of helicobacter pylori
TABLE 3
As can be seen from Table 3, in the male group, 200 patients had a statistical effect starting from 3 days of administration, and 110 patients had a 20% reduction in H.pylori detected. After 9 days of administration, 172 persons had a 40% reduction in H.pylori detected. 183 people after taking the medicine for 15 days have extremely obvious helicobacter pylori elimination, 192 people after taking the medicine for 20 days have 78% reduction of the helicobacter pylori detection, and 8 people after taking the lactobacillus plantarum have no capability of eliminating the helicobacter pylori.
In the female group, 200 people started to take the drug for 3 days, and 102 people of different ages who took the drug for 3 days were reduced by 20% in helicobacter pylori detection. After 9 days, 169 persons had a 40% reduction in H.pylori detected. 182 people take the medicine for 15 days to eliminate the helicobacter pylori very obviously, 191 people take the medicine for 20 days to reduce the helicobacter pylori by more than 76 percent through detection, and 9 people take the lactobacillus plantarum to fail to eliminate the helicobacter pylori.
In the adolescent male group, 150 people started to count the effect after taking for 3 days, and 62 people with different ages after taking for 3 days were reduced by 20% by detecting helicobacter pylori. After 9 days, there were 116 subjects who had a 40% reduction in H.pylori detected. After taking the medicine for 15 days, 136 people can eliminate helicobacter pylori obviously, and after taking the medicine for 20 days, 144 people can reduce helicobacter pylori by more than 80%, and total 6 people can not eliminate helicobacter pylori after taking the lactobacillus plantarum.
In the juvenile female group, 150 people started to count the effect after taking the drug for 3 days, and 68 people with different ages after taking the drug for 3 days were reduced by 20% by detecting helicobacter pylori. After 9 days of administration, there were 108 subjects who had a 40% reduction in H.pylori detected. After taking the medicine for 15 days, 136 people can eliminate helicobacter pylori obviously, and after taking the medicine for 20 days, 146 people can reduce the helicobacter pylori by over 75 percent through detection, and 4 people can not eliminate the helicobacter pylori after taking the lactobacillus plantarum.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
<110> Shandong Huanyi Biotechnology Ltd
<120> a probiotic composition for regulating intestinal tract and preventing constipation and a method for preparing the same
<130> GG18158548A
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1438
<212> DNA
<213> Lactobacillus plantarum
<400> 1
taaggcagct ggttcctaaa aggttacccc accgactttg ggtgttacaa actctcatgg 60
tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcatgc tgatccgcga 120
ttactagcga ttccgacttc atgtaggcga gttgcagcct acaatccgaa ctgagaatgg 180
ctttaagaga ttagcttact ctcgcgagtt cgcaactcgt tgtaccatcc attgtagcac 240
gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc ttcctccggt 300
ttgtcaccgg cagtctcacc agagtgccca acttaatgct ggcaactgat aataagggtt 360
gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca accatgcacc 420
acctgtatcc atgtccccga agggaacgtc taatctctta gatttgcata gtatgtcaag 480
acctggtaag gttcttcgcg tagcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 540
cccccgtcaa ttcctttgag tttcagcctt gcggccgtac tccccaggcg gaatgcttaa 600
tgcgttagct gcagcactga agggcggaaa ccctccaaca cttagcattc atcgtttacg 660
gtatggacta ccagggtatc taatcctgtt tgctacccat actttcgagc ctcagcgtca 720
gttacagacc agacagccgc cttcgccact ggtgttcttc catatatcta cgcatttcac 780
cgctacacat ggagttccac tgtcctcttc tgcactcaag tctcccagtt tccgatgcac 840
ttcttcggtt gagccgaagg ctttcacatc agacttaaaa aaccgcctgc gctcgcttta 900
cgcccaataa atccggacaa cgcttgccac ctacgtatta ccgcggctgc tggcacgtag 960
ttagccgtgg ctttctggtt aaataccgtc aatacctgaa cagttactct cagatatgtt 1020
cttctttaac aacagagttt tacgagccga aacccttctt cactcacgcg gcgttgctcc 1080
atcagacttt cgtccattgt ggaagattcc ctactgctgc ctcccgtagg agtttgggcc 1140
gtgtctcagt cccaatgtgg ccgattaccc tctcaggtcg gctacgtatc attgccatgg 1200
tgagccgtta ccccaccatc tagctaatac gccgcgggac catccaaaag tgatagccga 1260
agccatcttt caagctcgga ccatgcggtc caagttgtta tgcggtatta gcatctgttt 1320
ccaggtgtta tcccccgctt ctgggcaggt ttcccacgtg ttactcacca gttcgccact 1380
cactcaaatg taaatcatga tgcaagcacc aatcaatacc agagttcgtt cgactgca 1438
Claims (6)
1. A probiotic composition for regulating intestinal tract and preventing constipation is characterized in that lactobacillus plantarum powder and lactobacillus plantarum metabolite powder are used as active ingredients; the lactobacillus plantarum is preserved in China center for type culture Collection with the preservation number: CCTCC NO: m2018042; the composition also comprises a fungus powder carrier, wherein the fungus powder carrier comprises microcrystalline cellulose, medicinal corn starch, inulin and magnesium stearate; the composition comprises the following raw materials in parts by mass: 9-11 parts of lactobacillus plantarum powder, 4-6 parts of lactobacillus plantarum metabolite powder, 18-22 parts of microcrystalline cellulose, 80-120 parts of medicinal corn starch, 8-12 parts of inulin and 8-12 parts of magnesium stearate.
2. The composition of claim 1, wherein the composition comprises the following raw materials in parts by mass: 9.5-10.5 parts of lactobacillus plantarum powder, 4.5-5.5 parts of lactobacillus plantarum metabolite powder, 19-21 parts of microcrystalline cellulose, 90-110 parts of medicinal corn starch, 9-11 parts of inulin and 9-11 parts of magnesium stearate.
3. A method for preparing the composition of claim 1 or 2, wherein lactobacillus plantarum is subjected to expanded strain culture, primary seed culture, seed tank culture and fermentation culture to obtain lactobacillus plantarum ferment solution; centrifuging the lactobacillus plantarum zymocyte liquid to respectively collect lactobacillus plantarum bacterial sludge and centrifuged fermentation liquor, and respectively freezing, drying, crushing, concentrating, drying and crushing the lactobacillus plantarum bacterial sludge and the centrifuged fermentation liquor to obtain lactobacillus plantarum bacterial powder and lactobacillus plantarum metabolite powder; and mixing the lactobacillus plantarum powder and the lactobacillus plantarum metabolite powder with a bacterium powder carrier to obtain the composition.
4. The method according to claim 3, wherein the preparation method of the Lactobacillus plantarum fungus powder specifically comprises: and mixing the lactobacillus plantarum bacterium paste, sterile water, skimmed milk powder, fructo-oligosaccharide, glycerol, maltodextrin and isomaltooligosaccharide to obtain lactobacillus plantarum suspended bacterium liquid, and freeze-drying and crushing the suspended bacterium liquid to obtain lactobacillus plantarum bacterium powder.
5. The method of claim 4, wherein the suspension is freeze-dried at-35 ℃ to-30 ℃ for 2-5 hours, vacuum freeze-dried at-50 ℃ to-40 ℃ and crushed into 100 mesh lactobacillus plantarum powder.
6. Use of a composition prepared by the method of claim 3 in the preparation of a product having at least one of the following functions: 1) regulating intestinal flora; 2) preventing constipation; 3) can be used for treating diarrhea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910161548.7A CN109700033B (en) | 2019-03-04 | 2019-03-04 | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910161548.7A CN109700033B (en) | 2019-03-04 | 2019-03-04 | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109700033A CN109700033A (en) | 2019-05-03 |
| CN109700033B true CN109700033B (en) | 2021-06-04 |
Family
ID=66265614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910161548.7A Active CN109700033B (en) | 2019-03-04 | 2019-03-04 | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109700033B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899314A (en) * | 2006-07-07 | 2007-01-24 | 山东省农业科学院高新技术研究中心 | Process for preparing lactic acid bacteria oral liquor |
| CN104381694A (en) * | 2014-11-29 | 2015-03-04 | 郑州后羿制药有限公司 | Composite microecological preparation |
| CN104611275A (en) * | 2015-02-11 | 2015-05-13 | 成都与康科技有限公司 | Lactobacillus plantarum UCN-11 strain, and composition and application thereof |
| CN105112334A (en) * | 2015-04-27 | 2015-12-02 | 南昌大学 | Microecological preparation comprising lactobacillus plantarum |
| CN105106246A (en) * | 2015-08-20 | 2015-12-02 | 江南大学 | Lactobacillus plantarum ZS2058 and application thereof |
| CN106906165A (en) * | 2017-04-25 | 2017-06-30 | 重庆第二师范学院 | A kind of Lactobacillus plantarum and its application in the food for preparing Constipation |
| CN107099491A (en) * | 2017-07-05 | 2017-08-29 | 重庆第二师范学院 | Lactobacillus plantarum YS3 and its application in the food of Constipation is prepared |
| CN107099492A (en) * | 2017-07-05 | 2017-08-29 | 重庆第二师范学院 | Lactobacillus plantarum YS4 and its application in the food of Constipation is prepared |
| CN107267416A (en) * | 2017-07-05 | 2017-10-20 | 重庆第二师范学院 | Lactobacillus plantarum YS2 and its application in the food of Constipation is prepared |
| CN109234182A (en) * | 2017-12-22 | 2019-01-18 | 浙江大学 | Lactobacillus plantarum ZJUF T34 and its application |
-
2019
- 2019-03-04 CN CN201910161548.7A patent/CN109700033B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899314A (en) * | 2006-07-07 | 2007-01-24 | 山东省农业科学院高新技术研究中心 | Process for preparing lactic acid bacteria oral liquor |
| CN104381694A (en) * | 2014-11-29 | 2015-03-04 | 郑州后羿制药有限公司 | Composite microecological preparation |
| CN104611275A (en) * | 2015-02-11 | 2015-05-13 | 成都与康科技有限公司 | Lactobacillus plantarum UCN-11 strain, and composition and application thereof |
| CN105112334A (en) * | 2015-04-27 | 2015-12-02 | 南昌大学 | Microecological preparation comprising lactobacillus plantarum |
| CN105106246A (en) * | 2015-08-20 | 2015-12-02 | 江南大学 | Lactobacillus plantarum ZS2058 and application thereof |
| CN106906165A (en) * | 2017-04-25 | 2017-06-30 | 重庆第二师范学院 | A kind of Lactobacillus plantarum and its application in the food for preparing Constipation |
| CN107099491A (en) * | 2017-07-05 | 2017-08-29 | 重庆第二师范学院 | Lactobacillus plantarum YS3 and its application in the food of Constipation is prepared |
| CN107099492A (en) * | 2017-07-05 | 2017-08-29 | 重庆第二师范学院 | Lactobacillus plantarum YS4 and its application in the food of Constipation is prepared |
| CN107267416A (en) * | 2017-07-05 | 2017-10-20 | 重庆第二师范学院 | Lactobacillus plantarum YS2 and its application in the food of Constipation is prepared |
| CN109234182A (en) * | 2017-12-22 | 2019-01-18 | 浙江大学 | Lactobacillus plantarum ZJUF T34 and its application |
Non-Patent Citations (4)
| Title |
|---|
| Improvement of constipation and liver function by plant-derived lactic acid bacteria:a double-blind, randomized trial;Higashikawa F，等;《Nutrition》;20101231;第26卷(第4期);第239-254页 * |
| 乳酸杆菌的应用技术研究进展;柳玲玲，等;《耕作与栽培》;20151231(第5期);第69-71页 * |
| 微生态制剂临床应用研究进展;张蕾，等;《国外医学卫生学分册》;20091231;第36卷(第3期);第147-151页 * |
| 植物乳杆菌F1208对便秘大鼠胃肠道功能的影响;彭芝榕，等;《食品科学》;20131231;第34卷(第13期);第243-246页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109700033A (en) | 2019-05-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106834187B (en) | Bifidobacterium bifidum and application thereof | |
| CN111607538B (en) | Lactobacillus rhamnosus and application thereof in inhibiting helicobacter pylori | |
| CN109588726B (en) | Lactobacillus casei composition for improving sleep and preparation method thereof | |
| CN108823125A (en) | A kind of production method and application for treating sleep disturbance probiotics preparation | |
| CN109609420B (en) | Helicobacter pylori-resistant probiotic composition and preparation method thereof | |
| CN108743851A (en) | A kind of preparation method and compound probiotic powder of compound probiotic powder | |
| CN111548970B (en) | Lactobacillus crispatus capable of preventing and/or treating helicobacter pylori infection | |
| CN116024130B (en) | Lactobacillus fermentum A21215 for reducing blood uric acid and application thereof | |
| CN115838676A (en) | Traditional Chinese medicine fermentation by using novel lactobacillus plantarum strain and application | |
| CN111705012A (en) | Lactobacillus paracasei LC-37 with digestion promoting effect and application thereof | |
| CN111803533A (en) | Composition for reducing blood sugar and blood fat, preparation method and application thereof | |
| CN114540243A (en) | Lactobacillus rhamnosus capable of preventing and/or treating helicobacter pylori infection and application thereof | |
| CN116042470A (en) | Traditional Chinese medicine fermentation by using novel rhamnose cheese bacillus strain and application thereof | |
| US9040037B2 (en) | Culture medium for Lactobacillus acidophilus and Streptococcus faecalis, composition comprising the same and preparation method thereof | |
| CN116731891A (en) | Bifidobacterium breve B2798 and application thereof in preparation of probiotic preparation | |
| CN111471626A (en) | Lactobacillus helveticus capable of inhibiting helicobacter pylori and application thereof | |
| CN115119940A (en) | Application of Lactobacillus acidophilus LA85 and Bifidobacterium lactis BLA80 in inhibiting helicobacter pylori | |
| CN111035661A (en) | Application of lactobacillus plantarum | |
| CN108938789A (en) | A kind of more bacterium combination formulations of the adjusting intestinal microecology containing xylo-oligosaccharide | |
| US8338163B2 (en) | Culture medium for Bifidobacterium longum, composition comprising the same and preparation method | |
| CN109924507B (en) | Probiotics composition for conditioning emotion and preparation method thereof | |
| CN111345473A (en) | Probiotics composition containing yolk antibody IgY and application preparation | |
| CN109700033B (en) | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof | |
| CN109846044B (en) | Probiotic composition with functions of improving memory and enhancing immunity and preparation method thereof | |
| CN109757730B (en) | Composition with functions of reducing blood fat, blood pressure and blood sugar and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |